PCB-95 modulates the calcium-dependent signaling pathway responsible for activity-dependent dendritic growth.
OBJECTIVE: We tested the hypothesis that the CaMKI--CREB--Wnt2 signaling pathway couples NDL PCB-enhanced RyR activity to dendritic arborization.
METHODS AND RESULTS: [Ca.sup.2+] imaging of dissociated cultures of primary rat hippocampal neurons indicated that PCB-95 (2,2',3,5'6-pentachlorobiphenyl; a potent RyR potentiator), enhanced synchronized [Ca.sup.2+] oscillations in somata and dendrites that were blocked by ryanodine. As determined by Western blotting and quantitative polymerase chain reaction, PCB-95 also activated CREB and up-regulated Wnt2. Blocking CaMKK, CaMKI[alpha]/[gamma], MEK/ERK, CREB, or Wnt2 prevented PCB-95--induced dendritic growth. Antagonism of [gamma]-aminobutyric acid (GABA) receptors with bicuculline (BIC) phenocopied the dendrite-promoting effects of PCB-95, and pharmacological antagonism or siRNA knockdown of RyR blocked BIC-induced dendritic growth in dissociated and slice cultures of hippocampal neurons.
CONCLUSIONS: RyR activity contributes to dynamic remodeling of dendritic architecture in response to NDL PCBs via CaMKI--CREB--Wnt2 signaling in rats. Our findings identify PCBs as candidate environmental risk factors for neurodevelopmental disorders, especially in children with heritable deficits in calcium signaling associated with autism.
KEY WORDS: autism, [Ca.sup.2+], CaMKI, CREB, dendrites, developmental neurotoxicity, hippocampal MEK, neuronal connectivity, neurons, non-dioxin-like PCBs, ryanodine receptor, Wnt2. Environ Health Perspect 120:1003-1009 (2012). http://dx.doi.org/10.1289/ehp.1104833 [Online 25 April 2012]
Dendritic growth is strongly influenced by neuronal activity as evidenced by the remarkable effect of experience on the development and refinement of synaptic connections, which not only patterns neural circuitry during development but also underlies associative learning (Pittenger and Kandel 2003). The effects of neuronal activity on dendritic growth are mediated primarily, if not exclusively, by changes in intracellular calcium ion ([Ca.sup.2+]) levels (Lohmann and Wong 2005; Segal et al. 2000). We previously identified an NMDA (N-methyl-D-aspartate) receptor-mediated [Ca.sup.2+]-dependent signaling pathway that couples neuronal activity to dendritic arborization via sequential activation of [Ca.sup.2+]/calmodulin-dependent protein kinase kinase (CaMKK), [Ca.sup.2+]/calmodulin kinase-I (CaMKI), and extracellular signal-regulated kinase kinase (MEK/ERK) to enhance the CAMP response element binding protein (CREB)-mediated transcription of Wnt2 [wingless-type mouse mammary tumor virus (MMTV) integration site family member 2] (Wayman et al. 2006).
Polychlorinated biphenyls (PCBs) alter neuronal connectivity by interfering with normal patterns of dendritic growth and plasticity (Lein et al. 2007; Yang et al. 2009). Non-dioxin-like (NDL) PCBs promote dendritic growth via ryanodine receptor (RyR)-dependent mechanisms (Wayman et al. 2012); however, the downstream signaling events that mediate RyR-dependent effects of PCBs on dendritic arborization have yet to be described. In this study, we tested the hypothesis that the CaMKI--CREB--Wnt2 signaling pathway responsible for activity-dependent dendritic growth also couples NDL PCB-enhanced RyR activity to dendritic arborization. This hypothesis derives from observations that a) potentiation of RyR activity by NDL PCBs amplifies ionotropic glutamate receptor signaling (Gafni et al. 2004) and increases intracellular [Ca.sup.2+] levels in neurons (Wong et al. 1997, 2001); b) [Ca.sup.2+] signals generated by stimuli that promote dendritic growth, such as repetitive or prolonged depolarization (Emptage et al. 1999; Riquelme et al. 2011) or brain-derived neurotrophic factor (BDNF) (Adasme et al. 2011), are derived primarily from RyR-mediated [Ca.sup.2+] release; and c) RyR-dependent [Ca.sup.2+] signals activate CaM kinases, CREB, and transcription of genes encoding [Ca.sup.2+]-regulated proteins (Adasme et al. 2011; Dolmetsch et al. 1998; W Li et al. 1998). Our findings demonstrate that in cultured rat hippocampal neurons, RyR activity, triggered by either NDL PCBs or neuronal activity, contributes to dynamic remodeling of dendritic architecture via CaMKI-CREB-Wnt2 signaling.
A complete listing of reagents is provided in Supplemental Material, p. 3 (http://dx.doi.org/10.1289/ehp.1104833). Animals were treated humanely and with regard for alleviation of suffering according to protocols approved by the Institutional Animal Care and Use Committees of Oregon Health & Science University, University of California, Davis, and Washington State University, Pullman.
Cell culture. Hippocampal neurons were dissociated from postnatal day-1 Sprague-Dawley rats (Charles River Laboratories, Wilmington, MA) and cultured at high density ([10.sup.5] cells/[cm.sup.2]) in Neurobasal-A medium (Invitrogen, Carlsbad, CA) supplemented with B27 (Invitrogen) as described previously (Wayman et al. 2006). To visualize dendritic arbors, cultures were transfected at 6 days in vitro (DIV) with the plasmid-encoding microtubule-associated-protein-2B MAP2B (which labels the somatodendritic domain) fused to enhanced green fluorescent protein (EGFP) using Lipofectamine-2000 (Invitrogen) according to the manufacturer's protocol. A subset of cultures was simultaneously transfected with plasmids encoding dominant negative (dn) CaMKI (dnCaMKI), dnCREB (also referred to as ACREB), or Wnt inhibitory factor (Wif). PCBs or vehicle (DMSO at 1:1000 dilution) was added to the culture medium for 48 hr beginning at 7 DIV; in a subset of cultures, a CaMK kinase inhibitor (STO-609, 5 [micro]M) or a MEK inhibitor (U0126, 10 [micro]M) was also added to the medium during the same period.
Organotypic hippocampal slices from postnatal day-5 rats were cultured for 3 days as described previously (Lein et al. 2011). At 3 DIV, slice cultures were biolistically transfected with plasmid-encoding tomato fluorescent protein (TFP) using the Helios gene gun (Bio-Rad, Hercules, CA) per the manufacturer's directions. A subset of slice cultures was simultaneously transfected with siRNA (small interfering RNA) specific for RyR1 or RyR2. Slice cultures were exposed to vehicle, and PCBs were added to the culture medium during 4-6 DIV. A subset of cultures was also exposed to FLA365 [4-(2-aminopropy1)-3,5-dichloro-N,N-dimethylaniline] (10 [micro]M), which was added to the culture medium during the same period.
Dendritic morphology was quantified from digital images of green fluorescent protein-positive ([GFP.sup.+]) or [TFP.sup.+] neurons using Image J version 1.44p with the Neuron J plug-in version 1.42 to trace neurons (Meijering et al. 2004).
Calcium imaging. Spontaneous and electrically evoked [Ca.sup.2+] transients were measured in dissociated hippocampal neurons cultured on Greiner CELLSTAR[R] micro-clear wells (Sigma-Aldrich, St. Louis, MO). Cells were loaded with the [Ca.sup.2+]-sensitive dye Fluo-4 AM (5 [micro]M; Invitrogen) at 37[degrees]C for 30 min in imaging buffer consisting of 140 mM sodium choride (NaCl), 5 mM potassium chloride (KC1), 2 mM magnesium chloride ([MgCl.sup.2]), 2 mM calcium chloride ([CaCl.sup.2]), 10 mM HEPES, and 10 mM glucose, at pH 7.4, and supplemented with 0.05% BSA (bovine serum albumin). Cultures were washed three times with imaging buffer and transferred to the stage of an inverted Olympus IX70 microscope (Olympus America, Center Valley, PA) equipped with a 60 x 1.25 numeric aperture objective. Fluo-4 was excited at 494 nm using a DeltaRam illuminator (Photon Technologies Int'l., Birmingham, NJ); fluorescence emission was captured at 510 nm. Full-frame images were captured with an Evolve[R] cooled charge coupled device camera (Photometrics, Tucson, AZ) at 30 frames/sec (fps) using EasyRatioPro software (Photon Technologies Int'l.). In a subset of experiments, cultures were exposed to PCB-95 (2,2',3,5'6-penta-chlorobiphenyl; 2, 20, or 200 nM) from 7-9 DIV before loading with Fluo-4. After baseline recording, cultures were sequentially stimulated with electrical bipolar field pulses (0.5 millisec) at 1, 2.5, 5, and 10 Hz for 10 sec with 50-sec interstimulus rest periods using platinum electrodes connected to a Master 8 stimulator (A.M.Pd, Jerusalem, Israel). After acquisition, regions of interest were drawn freehand to encompass soma and distal dendrites (separated from the soma by a length of > 2 times the soma diameter). Movies were replayed to quantitatively measure changes in Fluo-4 fluorescence within the regions of interest.
At 7 DIV in separate experiments, spontaneous synchronized [Ca.sup.2+] transients were measured from hippocampal neurons that had not been previously exposed to PCB-95 before loading with Fluo-A. PCB-95 (200 nM) or vehicle (DMSO at 1:1,000 dilution) in imaging buffer was acutely introduced into cultures by continuous flow perfusion (~1 mL/min). In a subset of cultures, ryanodine (500 [micro]M; EMD Biosciences, Philadelphia, PA) was added to the culture medium 1 hr before loading the cells with Fluo-A to irreversibly block RyR channel activity (Buck et al. 1992; Zimanyi et al. 1992), which was verified by a challenge with 4-chloro-m-cresol (4CmC, 100 [micro]M; Sigma-Aldrich). Changes in cytoplasmic [Ca.sup.2+] were continuously recorded at 30 fps. [Ca.sup.2+] transients > 2 times the baseline amplitude were scored as oscillations. The number of oscillations was compared between vehicle and PCB-95-treated neurons. Transient amplitude was measured by normalizing peak change in Fluo-A fluorescence ([DELTA]F) to the fluorescence baseline ([F.sub.0]) and presented as mean [DELTA]F/[F.sub.0] for each neuron included in the analysis. Statistical comparisons were made using neurons (n = 30) obtained from three separate dissections. Statistical analysis was performed using unpaired Student's t test.
Quantitative polymerase chain reaction (qPCR). Total RNA was isolated from dissociated hippocampal neuron cultures (9 DIV) using Trizol (Invitrogen) according to manufacturer's instructions. Levels of Wnt2 mRNA were quantified by qPCR and normalized to GAPDH (glyceraldehyde 3-phosphate dehydrogenase gene) mRNA levels in the same sample. Primer sequences and a more detailed description are provided in Supplemental Material, p. 4 (http://dx.doi.org/10.1289/ehp.1104833).
PCB-95 alters [Ca.sup.2+] signals in cultured hippocampal neurons. We used real-time [Ca.sup.2+] imaging techniques to examine how subchronic (48 hr) or acute exposure to PCB-95 influences [Ca.sup.2+] signaling behaviors in cultured hippocampal neurons. Exposure to PCB-95 during 7-9 DIV caused a concentration-dependent attenuation of the amplitude of electrically evoked Calf transients (Figure 1A,B). This observation is consistent with previous findings that prolonged RyR activation by PCB-95 can deplete RyR-sensitive [Ca.sup.2+] stores (Gafni et al. 2004; Morton-Jones et al. 2008). Evidence that the [GABA.sup.A] (type A [gamma]-aminobutyric acid) receptor antagonist bicuculline (BIC) triggers dendritic growth via transient rather than prolonged activation of [Ca.sup.2+]-dependent signaling pathways (Wayman et al. 2006) suggests that PCB-95 may similarly trigger dendritic growth via amplification of [Ca.sup.2+] signals on a shorter time scale. We therefore examined [Ca.sup.2+] oscillatory behavior in PCB-naive hippocampal neurons loaded with the [Ca.sup.2+] indicator Fluo-A at 7 DIV immediately before focal applications of vehicle or PCB-95. Although vehicle alone had no influence (Figure 2A), acute application of PCB-95 at 200 nM, a concentration that promotes robust dendritic growth, significantly enhanced the amplitude and oscillations of Cali' transients in both soma and distal dendrites of cultured rat hippocampal neurons (Figure 2B, top traces). Amplification of [Ca.sup.2+] oscillations by PCB-95 was dependent on RyR activity as oscillations were completely prevented by pre-incubation of neurons with ryanodine (Figure 2B, bottom traces) under conditions previously shown to completely block gating of RyRs (500 [micro]M, 1 hr) (Buck et al. 1992; Zimanyi et al. 1992). To verify that ryanodine pretreatment in fact blocked all RyR channel activity in hippocampal neurons, a challenge of 4CmC [previously shown to activate RyR1 and RyR2, but not RyR3, channel activity (Fessenden et al. 2003)], was performed at the end of each recording. Ryanodine pretreatment resulted in a near complete block of 4CmC responses (Figure 2C).
CaMKI--CREB--Wnt2 signaling mediates PCB-95--induced dendritic growth.
Previous reports demonstrated that treatment of cultured hippocampal neurons with the CaMK kinase inhibitor STO-609 (2-5 [micro]M) inhibits CaMKI but not CaMKII (Wayman et al. 2004). STO-609 (5 [micro]M) selectively blocked PCB-95 effects on dendritic length (Figure 3A) and branching (Figure 3B) without altering basal dendritic growth. Expression of a previously characterized dnCaMKI (Wayman et al. 2004), also suppressed the stimulatory effects of PCB-95 on dendritic length and branching with no effect on basal parameters (Figure 3A,B). To determine which CaMKI isoforms may be involved in PCB-95-induced dendritic growth, previously characterized small hairpin RNA (shRNA) constructs that suppress individual CaMKI isoforms (Wayman et al. 2006) were expressed in hippocampal neurons. Expression of CaMKI[alpha] shRNA significantly reduced and CaMKI[gamma] shRNA blocked the stimulatory effects of PCB-95 on dendrites; in contrast, shRNAs for [BETA] and [delta] CaMKI had no effect (Figure 3C).
To probe downstream effectors of CaMKI, we first determined whether PCB-95-induced dendritic growth requires MEK/ERK signaling. Western blot analyses of phosphorylated ERK (pERK) levels did not consistently detect significantly increased pERK at 5-30 min after acute exposure of 7-DIV hippocampal cultures to PCB-95 (data not shown). However, the MEK inhibitor U0126 (10 [micro]M) completely blocked the stimulatory effects of PCB-95 on dendritic length and branching in the absence of any effect on basal dendritic growth (Figure 3D,E).
Western blot analyses revealed that, as previously reported (Wayman et al. 2006), BIC increased phosphorylation of the active site (Ser133) of CREB (Figure 4A,B). PCB-95 similarly elicited rapid (< 5 min) and sustained (> 30 min) CREB phosphorylation. Expression of either dnCREB or CREB-specific shRNA blocked the effects of PCB-95 on dendritic length and branching but had no effect on dendritic growth in vehicle control cultures (Figure 4C,D). We previously showed that expression of either dnCREB or shCREB (short hairpin CREB) does not induce nuclear pyknosis, increase levels of cleaved caspase-3, or significantly alter MAP2B-EGFP expression or dendritic morphology in hippocampal neurons grown in the presence of the serum-free supplement, B27 (Wayman et al. 2006).
Activity-dependent GRIM activation stimulates dendritic growth in part via increased Wnt2 expression (Wayman et al. 2006). Stimulation of hippocampal neurons with PCB-95 triggered a doubling of Wnt2 mRNA and this effect was blocked by treatment with the selective RyR antagonist FLA365 (Figure 4E). Expression of the Wnt inhibitor Wif, a secreted protein that binds Wnt and prevents it from activating its receptor Frizzled (Han and Lin 2005), completely suppressed PCB-induced dendritic growth without altering basal dendritic growth (Figure 4F,G).
BIC-induced dendritic growth requires RyR activity. These data raised the question of whether RyR activity is required for activity-dependent dendritic growth in the absence of PCBs. To address this question we used our previously described model of activity-dependent dendritic growth in which the [GABA.sub.A] receptor antagonist BIC promotes dendritic growth in cultured hippocampal neurons via activation of NMDA receptors (Wayman et al. 2006). Consistent with our previous studies, BIC (20 [micro]M) enhanced dendritic growth in cultures of dissociated hippocampal neurons (Figure 5A). BIC-induced dendritic growth was completely blocked by the RyR channel blocker FLA365 (Figure 5A). Expression of siRNA specific for either RyR1 or RyR2 [for characteriza-tion of siRNA specificity, see Supplemental Material, p. 4 and Figure S1 (http://dx.eloi.org/10.1289/ehp.1104833)] completely sup-pressed BIC stimulation of dendritic length and branching but had no effect on basal dendritic growth (Figure 5B--D). Similarly, BIC enhanced dendritic arborization of pyramidal neurons in hippocampal slice cultures, and this effect was blocked by FLA365 and by siRNA knockdown of RyR1 or RyR2 (Figure 5E).
The major finding of this study is that in cultured rat hippocampal neurons, RyR activity, triggered by either NDL PCBs or neuronal activity, contributes to dynamic remodeling of dendritic architecture via [Ca.sup.2+]dependent activation of the CaMKI--CREB--Wnt2 signaling pathway. We previously demonstrated that activity-dependent dendritic growth in hippocampal neurons is mediated by sequential activation of NMDA receptors, CaMKK, CaMKI, Ras, MEK/ERK, and CREB-dependent transcription of Wnt2 (Wayman et al. 2006). In this study, we confirmed that PCB-95 increases spontaneous [Ca.sup.2+] oscillations in hippocampal neurons in a manner blocked by ryanodine, and we identified these same signaling molecules as downstream effectors that couple RyR activation to dendritic growth. Previous studies in cultured neurons demonstrated that the commercial PCB mixture Aroclor 1254 (Al254) (Inglefield et al. 2001) and RyR activity (Kemmerling et al. 2007) increased neuronal levels of pCREB. We confirmed that PCB-95 activated CREB in hippocampal neurons as determined by Western blot analyses of pCREB expression, and we extended this finding to demonstrate that PCB-95 also up-regulated expression of the CREB-responsive gene Wnt2, as determined by qPCR. More importantly, we established a crucial role for not only CREB and Wnt2, but also CaMKK-CaMKI and MEK/ERK signaling in RyR-dependent dendritic growth using multiple experimental approaches induding pharmacological inhibition and expression of dominant-interfering or si/shRNA constructs specific for each of these signaling molecules. Consistent with our earlier studies of activity-dependent dendritic growth in cultured hippocampal neurons (Wayntan et al. 2006), shRNAs against both a and [gamma] CaMKI blocked the stimula-tory effects of PCB-95 on dendrites, whereas shRNAs for [beta] and [delta] CaMKI had no effect.
These data suggest a model in which PCB-95 potentiation of RyR activity promotes dendritic growth by triggering the same [Ca.sup.2+]-dependent signaling pathway linked to activity-dependent dendritic growth in hippocampal neurons (Wayman et al. 2006). We propose that PCB-95 interacts with RyR channels to increase the amplitude and frequency of intracellular [Ca.sup.2+] oscillations, which then trigger sequential activation of CaMKK, CaMKI, and MEK/ERK, resulting in the CREB-activated transcription of Wnt2 (Figure 6). Wnt2, a secreted glycoprotein, binds to the Frizzled family of receptors to activate the scaffold protein Dishevelled, which in turn stimulates dendritic growth via effects on the actin and microtubule cytoskele-ton mediated by [beta]-catenin and/or activation of the RhoGTPases Rac and/or JNK [members of the rho family of guanosine triphosphate (GTP) hydrolases] (Rosso et al. 2005; Yu and Malenka 2003). In our model, PCB-95 triggers this signaling pathway downstream of the NMDA receptor. However, RyRs are functionally coupled to NMDA receptors (Berridge 2006; Pessah et al. 2010), which considered in the context of our previous studies demonstrating that inhibition of NMDA receptors blocks activity-dependent dendritic growth (Wayman et al. 2006), suggested that RyR activity is required for not only PCB-induced dendritic growth, but also activity-dependent dendritic growth. In support of this hypothesis, we observed that the selective RyR antagonist, FLA365, as well as siRNA knockdown of either RyR1 or RyR2, completely blocked BIC-induced dendritic growth in both dissociated cultures of hippocampal neurons and hippocampal slice cultures.
Our observations add to the emerging experimental evidence supporting a central role for RyRs in neuronal excitability and use-dependent synaptic plasticity (Adasme a al. 2011; Bardo et al. 2006; Berridge 2006). Functional aspects of neuroplasticity, including long-term potentiation (Lu and Hawkins 2002; Wang et al. 1996), long-term depression (ST Li a al. 1998; Wang et al. 1997) and associative memory (Alkon et al. 1998; Baker et al. 2010; Edwards and Rickard 2006; Galeotti a al. 2008) are altered by experimental manipulation of RyR activity by ligands such as the RyR accessory FK506-binding protein (FKBP12) that directly modulate RyR or by agents that deregulate RyR by disrupting the RyR/FKBP12 complex. Spatial learning is tightly correlated with selective up-regulation of RyR expression (Adasme et al. 2011; Cavallaro et al. 1997; Yang et al. 2009; Zhao et al. 2000). Targeted deletion of RyR3 in mice causes impairments in social behavior (Matsuo a al. 2009) and deficits in contextual fear conditioning but improves spatial learning in the Morris water maze task (Futatsugi et al. 1999; Kouzu a al. 2000), whereas selective knockdown of RyR2 and RyR3 impairs avoidance memory processes (Galeotti et al. 2008). Previous studies have implicated a local release of [Ca.sup.2+] from intracellular stores in the maintenance of dendrites (Lohmann et al. 2002), the regulation of the motility of dendritic filopodia (Lohmann et al. 2005), and the increased size of dendritic spines (Korkotian and Segal 1999). More recently, inhibitory concentrations of ryanodine were reported to block BDNF-enhanced spine formation in primary hippocampal neurons (Adasme et al. 2011). However, our findings are the first to demonstrate that RyR activity is sufficient to promote dendritic growth and is necessary for activity-dependent dendritic growth.
Defective neuronal connectivity is a common pathological feature in most neuro-developmental disorders, including autism spectrum disorders (ASD) (Bourgeron 2009; Geschwind and Levitt 2007; Rubenstein and Merzenich 2003), which suggests that NDL PCBs are environmental risk factors for ASD. There is experimental evidence that developmental exposure to PCB-95 elicits some aspects of ASD, including an imbalance between excitation and inhibition in the auditory cortex of weanling rats (Kenet et al. 2007) and altered social behaviors in rats (Jolous-Jamshidi a al. 2010). However, it seems more likely that NDL PCBs interact with ASD-linked genes that converge on com-mon signaling pathways to increase ASD risk and/or severity. Emerging evidence suggests that defective neuronal connectivity associated with ASD is mediated in part by defects in neuronal [Ca.sup.2+] signaling (Lohmann 2009). A number of genes that encode proteins that have a primary role of generating intracellular [Ca.sup.2+] signals or are themselves tightly regulated by local fluctuations in [Ca.sup.2+] concentrations, including CREB and Wnt2, have been linked to ASD (Krey and Dolmetsch 2007; Levitt and Campbell 2009; Pessah and Lein 2008). More compelling are recent studies of a gain-of-function missense mutation in the L-type [Ca.sup.2+] channel Cav1.2 that causes Timothy syndrome, which has a 60% rate of co-morbidity with autism (Splawski et al. 2004), making it one of the most penetrant monogenic forms of ASD. Neurons differentiated from induced pluripotent stem cells derived from patients with Timothy syndrome revealed increased [Ca.sup.2+] oscillations and upregulated expression of genes linked to [Ca.sup.2+]-dependent regulation of CREB, including CaMK (Pasta et al. 2011). Such studies clearly establish these [Ca.sup.2+]-signaling molecules as possible convergence points for genetic variants linked to ASD risk and environmental factors such as NDL PCBs.
Adasme T, Haeger P, Paula-Lima AC, Espinoza I, Casas-Alarcon MM, Carrasco MA, et al. 2011. Involvement of ryanodine receptors in neurotrophin-induced hippocampal synaptic plasticity and spatial memory formation. Proc Natl Acad Sci USA 108(7):3029-3034.
Alkon DL, Nelson TJ, Zhao W, Cavallaro S. 1998. Time domains of neuronal Cave signaling and associative memory: steps through a calexcitin, ryanodine receptor, K+ channel cascade. Trends Neurosci 21(12):529-537.
Baker KD, Edwards TM, Rickard NS. 2010. A ryanodine receptor agonist promotes the consolidation of long-term memory in young chicks. Behav Brain Res 206(1)143-146.
Bardo S, Cavazzini MG, Emptage N. 2006. The role of the endoplasmic reticulum [Ca.sup.2+] store in the plasticity of central neurons. Trends Pharmacol Sci 27(2):78-84.
Berridge MJ. 2006. Calcium microdomains: organization and function. Cell Calcium 40(5-6):405-412.
Bourgeron T. 2009. A synaptic trek to autism. Curr Opin Neurobiol 19(2):231-234.
Buck E, Zimanyi I, Abramson JJ, Pessah IN. 1992. Ryanodine stabilizes multiple conformational states of the skeletal muscle calcium release channel. J Biol Chem 267(33):23560-23567.
Cavallaro S, Meiri N, Yi CL, Musco S, Ma W, Goldberg J, et al. 1997. Late memory-related genes in the hippocampus revealed by RNA fingerprinting. Proc Natl Aced Sci USA 94(18):9669-9673.
Dolmetsch RE, Xu K, Lewis RS. 1998. Calcium oscillations increase the efficiency and specificity of gene expression. Nature 392(6679):933-936.
Edwards TM, Rickard NS. 2006. Pharmaco-behavioural evidence indicating a complex role for ryanodine receptor calcium release channels in memory processing for a passive avoidance task. Neurobiol Learning Mem 86(1):1-8.
Emptage N, Bliss TV, Fine A. 1999. Single synaptic events evoke NMDA receptor-mediated release of calcium from internal stores in hippocampal dendritic spines. Neuron 22(1):115-124.
Fessenden JD, Perez CF, Goth S, Pessah IN, Allen PD. 2003. Identification of a key determinant of ryanodine receptor type 1 required for activation by 4-chloro-m-cresol. J Biol Chem 278(31):28727-28735.
Futatsugi A, Kato K, Ogura H, Li ST, Nagata E, Kuwajima G, et al. 1999. Facilitation of NMDAR-independent LTP and spatial learning in mutant mice lacking ryanodine receptor type 3. Neuron 24(3):701-713.
Gafni J, Wong PW, Pessah IN. 2004. Non-coplanar 2,2',3,5',6-pentachlorobiphenyl (PCB 95) amplifies ionotropic glutamate receptor signaling in embryonic cerebellar granule neurons by a mechanism involving ryanodine receptors. Toxicol Sci 77(1):72-82.
Galeotti N, Ouattrone A, Vivoli E, Norcini M, Bartolini A, Ghelardini C. 2008. Different involvement of type 1, 2, and 3 ryanodine receptors in memory processes. Learn Memory 15(5):315-323.
Geschwind OH, Levitt P. 2007. Autism spectrum disorders: developmental disconnection syndromes. Curr Opin Neurobiol 17(1):103-111.
Han C, Lin X. 2005. Shifted from Wnt to Hedgehog signaling pathways [Preview]. Mol Cell 17(3)121-322.
Inglefield JR, Mundy WR, Shafer II 2001. Inositol 1,4,5-triphosphate receptor-sensitive [Ca.sup.2+] release, store-operated [Ca.sup.2+] entry, and cAMP responsive element binding protein phosphorylation in developing cortical cells following exposure to polychlorinated biphenyls. J Pharmacol Exp Ther 297(2):762-773.
Jolous-Jamshidi B, Cromwell HC, McFarland AM, Meserve LA. 2010. Perinatal exposure to polychlorinated biphenyls alters social behaviors in rats. Toxicol Lett 199(2):136-143.
Kemmerling U, Munoz P, Muller M, Sanchez G, Aylwin ML, Klann E, et al. 2007. Calcium release by ryanodine receptors mediates hydrogen peroxide-induced activation of ERK and CREB phosphorylation in N2a cells and hippocampal neurons. Cell Calcium 41(5):491-502.
Kenet T, Froemke RC, Schreiner CE, Pessah IN, Merzenich MM. 2007. Perinatal exposure to a noncoplanar polychlorinated biphenyl alters tonotopy, receptive fields, and plasticity in rat primary auditory cortex. Proc Natl Acad Sci USA 104(18):7646-7651.
Korkotian E, Segal M. 1999. Release of calcium from stores alters the morphology of dendritic spines in cultured hippocampal neurons. Proc Natl Acad Sci USA 96(21):12068-12072.
Kouzu Y, Moriya T, Takeshima H, Yoshioka T, Shibata S. 2000. Mutant mice lacking ryanodine receptor type 3 exhibit deficits of contextual fear conditioning and activation of calcium/calmodulin-dependent protein kinase II in the hippocampus. Brain Res Mol Brain Res 76(1):142-150.
Krey JF, Dolmetsch RE. 2007. Molecular mechanisms of autism: a possible role for Cal' signaling. Curr Opin Neurobiol 17(1):112-119.
Lein PJ, Barnhart CD, Pessah IN. 2011. Acute hippocampal slice preparation and hippocampal slice cultures. Methods Mol Biol 758:115-134.
Lein PJ, Yang D, Bachstettar AD, Tilson HA, Harry BJ, Mervis RF, et al. 2007. Ontogenetic alterations in molecular and structural correlates of dendritic growth after developmental exposure to polychlorinated biphenyls. Environ Health Perspect 115:556-563.
Levitt P, Campbell DB. 2009. The genetic and neurobiologic compass points toward common signaling dysfunctions in autism spectrum disorders. J Clin Invest 119(4):747-754.
Li ST, Kato K, Mikoshiba K. 1998. Effect of calcineurin inhibitors on long-term depression in CA1 rat hippocampal neurons. 28th Annu Meet Soc Neurosci Abs 24:1815.
Li W, Llopis J, Whitney M, Ztokarnik G, Tsien RY. 1998. Cell-permeant caged InsP3 ester shows that [Ca.sup.2+] spike frequency can optimize gene expression. Nature 392(6679):936-941.
Lohmann C. 2009. Calcium signaling and the development of specific neuronal connections. Prog Brain Res 175:443-452.
Lohmann C, Finski A, Bonhoeffer T. 2005. Local calcium transients regulate the spontaneous motility of dendritic filopodia. Nat Neurosci 8(3):305-312.
Lohmann C, Myhr KL, Wong R0.2002. Transmitter-evoked local calcium release stabilizes developing dendrites. Nature 418(6894):177-181.
Lohmann C, Wong RO. 2005. Regulation of dendrite growth and plasticity by local and global calcium dynamics. Cell Calcium 37(51:403-409.
Lu YF, Hawkins RD. 2002. Ryanodine receptors contribute to cGMP-induced late-phase LTP and CREB phosphorylation in the hippocampus. J Neurophysiol 88(3):1270-1278.
Matsuo N, Tanda K, Nakanishi K, Yamasaki N, Toyama K, Takao K, et al. 2009. Comprehensive behavioral phenotyping of ryanodine receptor type 3 (RyR3) knockout mice: decreased social contact duration in two social interaction tests. Front Behav Neurosci 3:3; doi 10.3389/neuro.08.003.2009 [Online 7 May 2009].
Meijering E, Jacob M, Sarria JC, Steiner P, Hirling H, Unser M. 2004. Design and validation of a tool for neurite tracing and analysis in fluorescence microscopy images. Cytometry A 58(2):167-176.
Morton-Jones RT, Cannell MB, Housley GD. 2008. [Ca.sup.2+] entry via AMPA-type glutamate receptors triggers [Ca.sup.2+]-induced Cab release from ryanodine receptors in rat spiral ganglion neurons. Cell Calcium 43(4):356-366.
Pasca SP, Portmann T, Voineagu I, Yazawa M, Shcheglovitov A, Pasca AM, et al. 2011. Using iPSC-derived neurons to uncover cellular phenotypes associated with Timothy syndrome [Letter]. Nat Med 17(12):1657-1662.
Pessah IN, Cherednichenko 6, Lein PJ. 2010. Minding the calcium store: ryanodine receptor activation as a convergent mechanism of PCB toxicity. Pharmacol Ther 125(2):260-285.
Pessah IN, Lein PJ. 2008. Evidence for environmental susceptibility in autism: What we need to know about gene x environment interactions. In: Autism: Current Theories and Evidence (Zimmerman A, ed). Totawa, NJ: Humana Press, 409-428.
Pittenger C, Kandel ER. 2003. In search of general mechanisms for long-lasting plasticity: aplysia and the hippocampus. Philos Trans R Soc Lond B Biol Sci 358(1432):757-763.
Riquelme D, Alvarez A, Leal N, Adasme T, Espinoza I, Valdes JA, et al. 2011. High-frequency field stimulation of primary neurons enhances ryanodine receptor-mediated [Ca.sup.2+] release and generates hydrogen peroxide, which jointly stimulate NF-KB activity. Antioxid Redox Signaling 14(7):1245-1259.
Rosso SB, Sussman D, Wynshaw-Boris A, Salinas PC. 2005. Wnt signaling through Dishevelled, Rac and JNK regulates dendritic development Nat Neurosci 8(1):34-42.
Rubenstein JL, Merzenich MM. 2003. Model of autism: increased ratio of excitation/inhibition in key neural systems. Genes Brain Behav 2(5)255-267.
Segal M, Korkotian E, Murphy DD. 2000. Dendritic spine formation and pruning: common cellular mechanisms? Trends Neurosci 23(2):53-57.
Splawski I, Timothy KW, Sharpe LM, Decher N, Kumar P, Bloise R, et al. 2004. [Ca.sub.v]1.2 calcium channel dysfunction causes a multisystem disorder including arrhythmia and autism. Cell 119(1):19-31.
Wang Y, Rowan MJ, Anwyl R. 1997. Induction of LTD in the dentate gyrus in vitro is NMDA receptor independent, but dependent on [Ca.sup.2+] influx via low-voltage--activated [Ca.sup.2+] channels and release of [Ca.sup.2+] from intracellular stores. J Neurophysiol 77(2):812-825.
Wang Y, Wu J, Rowan MJ, Anwyl R. 1996. Ryanodine produces a low frequency stimulation-induced NMDA receptor-independent long-term potentiation in the rat dentate gyrus in vitro. J Physiol 495 (Pt 3):755-767.
Wayman GA, Impey S, Marks D, Saneyoshi T, Grant WF, Derkach V, et al. 2006. Activity-dependent dendritic arborization mediated by CaM-kinase I activation and enhanced CREB-dependent transcription of Wnt-2. Neuron 50(6):897-909.
Wayman GA, Kaech S, Grant WF, Dava re M, lmpey S, Tokumitsu H, et al. 2004. Regulation of axonal extension and growth cone motility by calmodulin-dependent protein kinase I. J Neurosci 24(15):3786-3794.
Wayman GA, Yang D, Bose DD, Lesiak A, Ledoux V, Bruun D, et al. 2012. PCB-95 promotes dendritic growth via ryanodine receptor--dependent mechanisms. Environ Health Perspect 120:997-1002.
Wong PW, Brackney WR, Pessah IN. 1997. ortho-Substituted polychlorinated biphenyls alter microsomal calcium transport by direct interaction with ryanodine receptors of mammalian brain. J Biel Chem 272(24)15145-15153.
Wong PW, Garcia EF, Pessah IN. 2001. ortho-Substituted PCB95 alters intracellular calcium signaling and causes cellular acidification in PC12 cells by an immunophilin-dependent mechanism. J Neurochem 76(2):450-463.
Yang D, Kim KH, Phimister A, Bachstetter AD, Ward TR, Stackman RW, et al. 2009. Developmental exposure to polychlorinated biphenyls interferes with experience-dependent dendritic plasticity and ryanodine receptor expression in weanling rats. Environ Health Perspect 117:426-435.
Yu X, Malenka RC. 2003.[beta]-catenin is critical tor dendritic morphogenesis. Nat Neurosci 6(11):1169-1177.
Zhao W, Meiri N, Xu H, Cavallaro S, Quattrone A, Zhang L, et al. 2000. Spatial learning induced changes in expression of the ryanodine type II receptor in the rat hippocampus. FASEB J 14(2):290-300.
Zimanyi I, Buck E, Abramson JJ, Mack MM, Pessah IN. 1992. Ryanodine induces persistent inactivation of the [Ca.sup.2+] release channel from skeletal muscle sarcoplasmic reticulum. Mol Pharmacol 42(6):1049-1057.
Address correspondence to P.J. Lein, UC Davis School of Veterinary Medicine, Department of Molecular Biosciences, One Shields Ave., 1120 Haring Hall, Davis, CA 95616, USA. Telephone: (530) 752-1970. Fax: (530) 752-4698. E-mail: firstname.lastname@example.org
* These authors contributed equally to this work.
Supplemental Material is available online (http://dx.doi.org/10.1289/ehp.1104833).
This work was supported by grants from the National Institutes of Health (R01 ES014901, R01 ES017425, P42 ESO4699, ROI MH086032, P01 ES011269, and T32 ES007060); the Hope for Depression Research Foundation; and an unrestricted gift grant from the J.B. Johnson Foundation.
The authors declare they have no actual or potential competing financial interests.
Received 8 December 2011; accepted 2 April 2012.
Gary A. Wayman, (1) * Diptiman D. Bose, (2) * Dongren Yang, (2) Adam Lesiak, (1) Donald Bruun, (2) Soren Impey, (3) Veronica Ledoux, (4) Isaac N. Pessah, (2) and Pamela J. Lein (2,4)
(1) Program in Neuroscience, Department of Veterinary and Comparative Anatomy, Pharmacology and Physiology, Washington State University, Pullman, Washington, USA; (2) Department of Molecular Biosciences, University of California, Davis, Davis, California, USA; (3) Oregon Stem Cell Center, and (4) Center for Research on Occupational and Environmental Toxicology, Oregon Health & Science University, Portland, Oregon, USA
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|Author:||Wayman, Gary A.; Bose, Diptiman D.; Yang, Dongren; Lesiak, Adam; Bruun, Donald; Impey, Soren; Ledoux|
|Publication:||Environmental Health Perspectives|
|Date:||Jul 1, 2012|
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