PATTERNS OF PULMONARY MORPHOLOGICAL LESIONS SEEN AT AUTOPSY.
Introduction: Chronic respiratory diseases are a group of chronic diseases affecting the airways and the other structures of the lungs. Hundreds of millions of people around the world suffer from preventable chronic respiratory diseases. Prompt investigation and diagnosis are essential to improving patient survival.
Objective: The objective of this study was to observe the frequencies of different lung diseases on the basis of histopathological examination of lung tissue specimens removed at autopsy. This study was a non-interventional, cross sectional and was conducted on 810 specimens of lungs at Pathology department of Allama Iqbal Medical College, Lahore in collaboration with the Forensic Medicine department of King Edward Medical University, Lahore.
Methods: Relevant autopsy data was recorded in a proforma. The tissue specimens were fixed and processed. Paraffin sectioning was done followed by Haematoxylin and Eosin staining. The sections were then examined by a panel of consultant Histopathologists. Autopsies of 810 subjects with respiratory diseases were reviewed, and the following data were obtained: age, sex and cause of death.
Results: During a period of one year, a total of 810 lungs and hilar lymph nodes specimens from autopsy subjects were studied. Maximum numbers of cases (68.14%) were in 20 49 years age group. The commonest cause of death (42.96%) was tuberculosis. On microscopic examination of the sections from the lungs, there were 348 cases of tuberculosis, 324 out of 810 (40%) cases of emphysema and silicosis was present in 89 (11%) cases. Squamous metaplasia was present in 49 (6%) and Pneumonia in 4% cases.
Conclusion: Advances in diagnostic technology have not reduced the value of autopsy and a goal directed autopsy remains a vital component for the study and evaluation of the disease process. Emphysema and tuberculosis are quite common in our population.
Key words: Emphysema, silicosis, tuberculosis.
Chronic respiratory diseases are a group of chronic diseases affecting the airways and the other structures of the lungs.1-5 Hundreds of millions of people around the world suffer from preventable chronic respiratory diseases.6-8 Prompt investigation and diagnosis are essential to improving patient survival. The clinical and radiological findings in pulmonary diseases are nonspecific, and prompt pathology investigation and diagnosis are essential to improve patient survival.9-12 In this context, the complexity of clinical presentations makes diagnosis a constant challenge for the clinician. Despite recent advances, most types of diagnostic support are still expensive. Clinicians often initiate treatment to avoid the rapid progression of the disease and to spare the patient from more invasive procedures.13-18
Therefore, it is important to determine the leading causes of death to establish correct prophylactic actions, which is the least expensive strategy for preventing further pul-monary dysfunction and avoiding the need for lung biopsies.19-26
An autopsy is a medical procedure that consists of a thorough examination performed on a body after death, to evaluate disease or injury that may be present and to determine the cause and manner of a person's death. An autopsy may be required in deaths that may have medical and legal issues.27-31
Pathologic examination of autopsy lungs gives valuable information such as various stages of fibrosis, including early patchy fibrosis and honey combing lesions, and their distribution and progression in the lungs.32-38
The aim of this study was to present the pulmonary histopathological alterations identified in autopsies of patients who died from respiratory diseases, as well as to determine whether underlying diseases and associated comorbidities increase the risk of developing specific histopathological patterns. MATERIALS AND METHODS
The study was conducted at Pathology department of Allama Iqbal Medical College, Lahore in collaboration with the Forensic Medicine department of King Edward Medical University, Lahore.
Duration of Study One year.
The study was conducted on 810 specimens of lungs and hilar lymph nodes to find out the frequency of various pulmonary lesions at autopsy.
Non-probability convenience sampling was done.
Sample Selection Inclusion Criteria
Autopsy subjects above 10 years of age and of both sexes were included in the study. The subjects were selected irrespective of cause of death.
This study was a non-interventional, cross sectional study.
Data Collection Procedure
Autopsy information regarding name, age, address and history of smoking were obtained from the relatives of the deceased.
Specimen Collection and Preservation
The lungs were fixed in 10% formalin prepared in 0.9% saline for at least 4 weeks. Irrespective of the presence or absence of morphologically demonstrable lesions, a minimum of five sections per lung were studied (total 10 sections per person). Paraffin embedded tissue sections (5 mm thick) were assessed using haematoxylin and eosin stain.
Data Analysis Plan
Data was entered and analyzed on SPSS version 10. descriptive statistics were calculated. Age was presented as mean +- SD. Sex and cause of death were shown as percentages. Pattern of pulmonary lesions were also presented as percentages.
During a period of one year from January 2007 to December 2007, a total of 810 specimens of lungs from autopsy subjects were studied, in collaboration with Forensic Medicine department of King Edward Medical University Lahore, at the Histopathology section of the Pathology department of Allama Iqbal Medical College.
The patient characteristics were as follows: The study included 810 autopsy subjects. Maximum number of cases (68.14%) was in 20 49 years age group (Table 1) in the present study. Significantly greater (p less than 0.001) number of males (552 out of 810) were autopsied (Table 2).
Microscopic examination of the sections from the lungs showed that there were 324 (40%) cases of emphysema (Fig. 1). Majority of these subjects (251 out of 324), were in age group 20 49 years. Emphysema was seen in significantly greater (p less than 0.005) number of males (251 out of 324, 77.50), and majority (259 out of 324, 79.94%) were smokers.
Granulomatous lesions were found in 154 out of 810 cases (19%). Majority (84.41%) were below the age 50 and significantly greater (p less than 0.01) number (122 out of 154, 79.22%) number of males were affected.
Fig. 1: Photomicrograph from the lung shows emphysema.
Fig. 2: Photomicrograph show granulomatous reaction in silicosis of lung (H and E).
There were 89 (11%) cases of silicosis (Fig. 2). Majority of silicosis i.e. 57 (64%) were in age group 30 49 years. Greater number of males i.e. 65 out of 89 (73%) were affected.
Squamous metaplasia of bronchiolar epithelium was seen in 49 (6%) of 810 cases. Majority of these lesions (41 out of 49, 83.67%) were seen in subjects below the age of 50 years and males with history of smoking were significantly greater (p less than 0.001) greater than the females and nonsmokers.
Pneumonia was present in 32 out of 810 cases (4%) Majority (75%) cases were below the age of 50 years and there was no sex difference.
Table 1: Age Distribution in different cases (n = 810).
Table 2: Distribution of Pulmonary Diseases on autopsy (n = 810).
Table 3: Sex distribution in different cases (n = 810).
Fig. 3: Distribution pattern of pulmonary diseases.
The results of this study show that among the pulmonary diseases, Emphysema is the commonest disease affecting males of 20 49 year age group. We found 324 cases of Emphysema which make 40% of the total cases studied. This result is very close to the findings of a study conducted by Latif and his coworkers, in which they found Emphysema in 43.0% cases.39 Similarly, Niazi in her "Morphological study of pulmonary embolism in autopsy cases" found emphysema in 30.89% cases. Significantly greater (p less than 0.005) numbers (77.5%) of emphysema were found in smokers.40 This shows the causal relation of smoking in the pathogenesis of emphysema.41
Table 1: Age Distribution in different cases (n = 810).
Age Group###No. of Diseased Cases###Percent###No. of Nondiseased Cases###Percent###Total###Percent %
60 and Above###47###7.3###20###12.0###67###8.3
Table 2: Distribution of Pulmonary Diseases on autopsy (n = 810).
Diagnosis###No. of Cases###Percent %
Pulmonary lesions Present###648###80
Pulmonary lesions Absent###162###20
Table 3: Sex distribution in different cases (n = 810).
Sex###No. of Cases###Percent %
In our study, there were one hundred and fifty four cases (19.0%) of tuberculosis. One hundred and thirty cases (84.41 %) were below 50 years age group and twenty four (15.59 %) were above 50 years. The findings were comparable to those found by Rasul et al in their study.43 Males were significantly (p less than 0.03) more commonly affected as compared with females. This male preponderance is reported by other workers as well.44
We found 89 (11.0 %) cases of silicosis. This finding is comparable to the prevalence of silicosis in some of the developing countries in persons occupationally exposed to silica dust. The prevalence of silicosis in Bolivia, Brazil, China, Egypt, Korea and Thailand in miners ranged from 1.7 to 12.5 %45. The prevalence of silicosis reported by Churchyard et al in South Asian gold miners is 18.3 to 19.9% as these are exposed to higher intensity of respirable dust and such miners spend longer periods in continuous employment in dusty jobs.46 This indicates that in our country, air pollution has reached to such an extent which is comparable to the dust concentrations present in mines of the developing countries. Maximum number of silicotic cases (57 out of 89, 64%) were in the age group 0f 30 49 years. Norboo et al also reported nearly the same age group in India.47 In our study there were 65 males and 24 females of silicoic cases.
This finding is in disagreement with that of Norboo et al whereby women appear to be more commonly affected than men. This difference is due to the reason that they conducted their study in two villages of India where women were more heavily exposed to dust in the course of their work. But in our autopsy study of medico legal cases, men were more commonly involved.
Squamous metaplasia of bronchioles was seen in 6.0% cases (49 out of 810). This finding is in disagreement to that of Peters et al who examined six bronchial biopsy specimens from 99 subjects and found Squamous metaplasia in 66 cases (66.66%).48 This difference is due to the fact that they examined six standard biopsy sites and their patients were cigarette smokers with long history of pack years. Subjects with Squamous metaplasia had history of smoking in 41 (83.67%) cases. This clearly indicates the higher prevalence of Squamous metaplasia in cigarette smokers. Tsuchiya E et al in their study of "Incidence of Squamous metaplasia in large bronchi of Japanese lungs: relation to pulmonary carcinomas of various subtypes" concluded that Squamous metaplasia may be associated with the development of Squamous cell carcinoma but would appear not to be an obligatory step in the development of this neoplasm.49
Hirsh FR et al in their study also emphasized that Squamous metaplasia is a common finding especially as a response to cigarette smoking.
There were 32 cases (4 %) of pneumonia in the present study. Niazi found 22 cases (17.88 %) in her study of pulmonary embolism in a total of 123 medico-legal autopsy cases. The higher frequency of pneumonia in her study could be due to the reason that her study included 30% hospitalised patients who remained admitted to the hospital for a longer or shorter period of time.
1. Despite recent advances in diagnostic technology, the autopsy has remained an important complementary tool for identifying and understanding chronic respiratory diseases.
2. Emphysema and tuberculosis are quite common in our population. Emphysema and silicosis are clearly due to smoking and environmental air pollution. This highlights the role of environmental dust in causation of silicosis.
The authors are grateful to the administration of Sharif Medical Complex and SIMS for supporting this project.
1. Alexandre de MS, Aline DR, Mauro C, Edwin RP, Ceclia F, Vera LC. Demographic, etiological, and histological pulmonary analysis of patients with acute respiratory failure: a study of 19 years of autopsies. Clinics 2011; 66 (7): 1193-119.
2. Canzian M, Soeiro AM, Taga MF, Barbas CS, Capelozzi VL. Correlation between surgical lung biopsy and autopsy findings and clinical data in patients with diffuse pulmonary infiltrates and acute respiratory failure. Clinics. 2006; 61: 425-32.
3. Parambil JG, Myers JL, Aubry MC, Ryu JH. Causes and prognosis of diffuse alveolar damage diagnosed on surgical lung biopsy. Chest. 2007; 132: 50-57.
4. McManus JFA. The Autopsy as Research. JAMA 1965; 193: 152.
5. Brooks JP, Dempsey J. How can hospital autopsy rates be increased. Arch-Pathol-Lab-Med 1991; 115: 1107-11.
6. Gross JS, Neufeld RR, Libow LS, Gerber I, Rodstein M. Autopsy study of the elderly institutionalized patient. Review of 234 autopsies. Arch Intern Med. 1998; 148: 173-6.
7. Tai DYH, El-Bilbeisi H, Tewari S, Mascha EJ, Wiedemann HP, Arroliga AC. A study of consecutive autopsies in a medical ICU: a comparison of clinical cause of death and autopsy diagnosis. CHEST 2001; 119: 530-6.
8. Snell RS. Clinical Anatomy by Regions.8th ed. India. Wolkers Kluwer; 2008: 77-144.
9. Sinnatamby CS. Last's Anatomy Regional and applied. 11th ed China,Elsevier, 2009: 185-228.
10. Sadler TW. Langman's Medical Embryology10th ed. New Dehli. Wolters Kluwer, 2007: 195-201.
11. Guyton AC, Hall JE. Textbook of Medical Physiology 11th ed.India.Elsevier,2006:110-24.
12. Hussain AN, Kumar V. The Lung. In:Robbins, Cotran eds. Pathologic basis of disease. 7th Ed. New Dehli:Elsevier; 2006: P 711-72.
13. Becklake MR. The mineral dust diseases. Tuber Lund Dis. 1992; 73: 13-20.
14. Ghosal R, Kloer P, Lewis KE. A review of novel biological tools used in screening for the early detection of lung cancer. Postgraduate Medical Journal 2009; 85: 358-63.
15. Broughton WA, Bass Jb. Tuberculosis and disease caused by atypical mycobacteria. In: Albert RK, Spiro SG, Jett JR eds. Comprehensive respiratory medicine. 1st ed. London: Mosby; 1999: P1-16.
16. Kumar V, Abbas A K, Fausto N, Mitchell RN. Robbins Basic Pathology. 7th ed. India. Elsevier; 2007: 717-9.
17. Fact sheets tuberculosis: general information. CDC. 2008. www. cdc.gov/tb/pubs/tbfactsheets/tb.htm.
18. Tiwari RR, Sharma YK, Saiyed HN. Tuberculosis among workers exposed to free silica dust. Indian J Occup Environ med 2007; 11: 61-4.
19. World Health Organization (WHO). Tuberculosis Fact sheet N 104 Global and regional incidence. Available online at www.who.int/mediacentre/factsheets/fs104/en/index .html. March 2006
20. World Health Organization. TB DOTS guidelines Pakistan. 2005. www. who.int/globalatlas/predefinedReports/TB/index.
21. USAID. Infectious Diseases. Pakistan. Available online at www.usaid.gov/our_work/global_health/id/tubercul osis/countries/asia/pakistan_profile.html.
22. McLoud Tc. Occupational lung diseases. Radiol Clin north Am 1991; 29: 931-41.
23. Stark P, Jacobson F, Shaffer K. Standard imaging in silicosis and Coal worker's pneumoconiosis. Radiologic Clinics of North America. 1992; 30: 1147-54.
24. Corrin B, eds. The Lungs.3rd Ed. London; Churchill Livingstone, 1990: 235-61.
25. Cohen R, Velho V. Update on respiratory disease from coal mine and silica dust. Clin Chest Med 2002; 23: 811-26.
26. Stark P, Jacobson F, Shaffer K,. Standard imaging in silicosis and coal worker's pneumoconiosis. Radiological Clinics of North America 1992; 30: 1147-54.
27. Heppleston AG. Minerals, Fibrosis and the Lung. Environmental Health Perspectives 1991; 94: 149-68.
28. Gurney JW. Cross sectional physiology of the lung. Radiology 1991; 1: 1-10.
29. Roggli VL, Shelburne JD. Pneumoconiosis and minerals and vegetables. In: Dail DH, Hamman SP eds. Pulmonary Pathology. 2nd Ed. New York. SpringerVerlag, 1994: 868-9.
30. YI Q, Zhang Z. The survival analysis analyses of 2738 patients with simple pneumoconiosis Occup Environ Med 1996; 53: 129-35.
31. Cowie RL. The epidemiology of tuberculosis in gold miners with silicosis. Am J Respire Crt Care Med 1994; 150: 1460-2.
32. Beckett WS. Occupational respiratory diseases. NEJM 2000; 342: 406-13.
33. Saleem N, Saleem M, Dil AS. Monitoring gaseous pollutants at Murree Highway. Pakistan J Health 1991; 28: 3-5.
34. Parmeggiani L, ed. Encyclopedia of Occupational Health and Safety. 3rd ed. Geneva: International Labour Office. 1983: 95-102.
35. Davies FG, Bassett WH, eds. Clay's Handbook of Environmental Health. 14th ed. London: HK Levis and co Ltd, 1977: 524-84.
36. Mishra V. Health effects of air pollution. Available online at www.populationenvironmentresearch.org/html.
37. Gamble M. The Haematoxylins. In: Bancroft JD and Gamble M, eds. Theory and Practice of histological techniques. 6th Ed., New York: Churchill Livingstone 2008: 121-33.
38. Rapheal SS, Hyde TA, Mellor LD eds. Lynch's Medical Laboratory Technology. 4th Ed Philadelphia. W.B. Saunders, 1983: 14-39.
39. Latif Z, Nagi AH, Emphysema in 100 cases: A postmortem study. Pakistan Journal of Pathology 1992; 3:11-15.
40. Niazi S. Morphological study of Pulmonary Embolism in autopsy cases. [Thesis]. Lahore: University of the Punjab, 1989.
41. Motley HL, Yanda R. Environmental air pollution, emphysema and ionized air. Chest 1966; 50: 343-52.
42. Latif Z, Nagi AH. Emphysema in 100 cases; a postmortem study. Pakistan Journal of Pathology 1992; 3: 11-5.
43. Rasul S, Akhtar MJ, Khan SA, Chaudrhy MK, Tahir TM. Radiological features of Pulmonary Tuberculosis: An assessment of 113 cases. Biomedica 1995; 11: 23-7.
44. Khan SA. Thesis writing. Lahore. Postgraduate Medical Institute 1993: 30-58.
45. Van Sprundel MP. Pneumoconioses. The situation in developing countries. Exp Lung Res 1990; 16: 5-13.
46. Churchyard GJ, Ehrlich R, teWaterNaude JM, Pemba L, Dekker K, Vermeijs M et al. occupational and Environmental medicine 2004; 61: 811-6.
47. Norboo T, Angchuk PT, Yahya M, Kamat SR, Pooley FD, Corrin B et al. Silicosis in a Himalayan village population: role of environmental dust. Thorax 1991; 46: 341-3.
48. Peters J, Morice R, Benner E, Lippman S, Lukeman J, Lee J et al. Squamos metaplasia of the bronchial mucosa and its relationship to smoking. Chest 1993; 103: 1429-32.
49. Tsuchiya E, Kitagawa T, Oh S, Nakagawa K, Matsubara T, Kinoshita I et al. Incidence of squamous metaplasia in large bronchi of Japanese lungs: relation to pulmonary carcinomas of various subtypes. Jpn J Cancer Res. 1987; 78: 559-64.
Department of Pathology, 1Sharif Medical and Dental College Services Institute of Medical Sciences
Rehbar Medical College and 4Fatima Jinnah Medical College, Lahore
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