Printer Friendly

Oxidative Status in Patients with Benign Paroxysmal Positional Vertigo.

INTRODUCTION

Benign paroxysmal positional vertigo (BPPV) is the most frequent peripheral vestibular disorder; it is particularly seen among older patients suffering from vertigo [1]. Brief vertigo attacks in and imbalance symptomatic of BPPV are caused by otoconia freely floating within the semicircular canals [2]. Vertigo occurs after specific head movements and has the characteristics of nystagmus, with respect to latency time, fatigability, and transiency. Canalithiasis and cupulolithiasis are the most likely mechanisms underlying BPPV. Although any of the three semicircular canals may be involved, canalithiasis of the posterior semicircular canal is the underlying cause in at least 85% of patients [3-5].

Oxidative stress, which is defined as excessive production of reactive oxygen species (ROS) that is not counterbalanced by adequate endogenous and exogenous antioxidant defenses, causes cellular dysfunction and is a risk factor for microvascular injury [6]. Several studies have shown an elevation in oxidative stress levels in different pathologies, with higher-than-control levels of biomarkers such as modified lipids, proteins, and nucleic acids, reduction in antioxidant capacity, and increased ROS production by leukocytes. In otolaryngology, the relationship between oxidative stress and laryngeal cancer, hearing loss, rhinosinusitis, otitis media, chronic tonsillitis, and other conditions has been investigated [7-10]. In a recent study, it has been revealed that calcium metabolism and its relationship with oxidative stress may play a role in the development of BPPV [7].

The aim of this prospective study was to evaluate the role of oxidative stress, using native thiol thiol/disulfide (SH/SS) homeostasis as a novel indicator, in the etiology of BPPV.

MATERIALS and METHODS

Patients and Controls

This prospective two-center study was conducted from June 2017 to July 2017 at the Department of Otolaryngology Head and Neck Surgery of the Umraniye Training and Research Hospital and at the Department of Otolaryngology Head and Neck Surgery of Icerenkoy Hospital, Bayindir Health Care group. The study was approved by the Ethics Committee of Ethics Committee of Umraniye Training and Research Hospital (number 2017/76). Written informed consent was obtained from all participants recruited for the study.

Totally, there were 62 participants: 31 patients with BPPV (study group) and 31 healthy individuals without any cochleovestibular disorders (control group).

Patients with hearing loss, a history of otologic surgery, neurologic disorders, smoking, malignancy, autoimmune disorders, hypertension, endocrine disorders including diabetes mellitus and hypothyroidism, cardiovascular disease, a history of antiaggregant therapy, infectious diseases, or other inflammatory conditions were excluded.

Vestibular and Acoustic Evaluation

All participants underwent a complete otorhinolaryngologic examination, including a neuro-otologic examination. The otologic examination included otomicroscopy to visualize the tympanic membrane for vesicles attributable to herpes zoster infection and to determine the presence of chronic ear disease or retraction pockets with cholesteatoma. The Valsalva maneuver and pushing on the tragus cartilage (fistula test) were performed; it was evaluated whether either would induce a vertigo attack, which would have suggested a perilymphatic fistula. All participants underwent pure-tone audiometry. The neurologic examination focused on gait, balance, and coordination. Gait and balance were assessed using Romberg's sign and the Fukuda stepping test. The presence of cerebellar signs was evaluated to exclude central pathologies.

For the diagnosis of BPPV in patients whose symptoms worsened with sudden head movements, videonystagmography-assisted Dix--Hallpike and supine roll tests (Pagnini--McClure maneuver) were performed. The characteristic pattern of BPPV nystagmus and the history of the disease were recorded for all patients in the study group. Only those with posterior canal BPPV were included. Patients in the study group had geotropic, torsional nystagmus beating toward the undermost ear. The nystagmus duration was less than 60 s, with a latency of a few seconds and a decline in response upon performing repeat maneuvers. Epley's repositioning maneuver was performed in the treatment of patients with BPPV. The Dix--Hallpike test was repeated for all patients with BPPV 2 days after the diagnosis of posterior canal BPPV; Epley's repositioning maneuver was repeated if patients complained of vertigo, nausea, or vomiting or if nystagmus was determined during the Dix--Hallpike test. The latter test was performed in control individuals 21 days after the first hospital admission.

Blood Sample Collection

Peripheral venous blood samples were obtained from the patients with BPPV upon first hospital admission (during the vertigo attack) and 21 days after treatment. Blood samples were obtained from the healthy controls during a routine medical examination. Plain tubes were used to collect blood from the patients and controls. The samples were centrifuged for 10 min at 1,500 g, after which the serum was separated and stored at -80[degrees]C until further analysis. Serum SH, total thiol (TT), and SS levels were analyzed in these samples, and SS/SH, SS/TT, and SH/TT ratios were calculated according to the method of Erel and Neselioglu [11].

Statistical Analysis

The NCSS 2007 program (NCSS, Kaysville, Utah, USA) was used for statistically evaluating the data. The mean, standard deviation, median, minimum, maximum, frequency, and percentage values served as descriptive statistics. Normally distributed data were compared between the groups with the independent samples t-test. The paired samples t-test was used for within-group analysis of normally distributed data, and Pearson's chi-squared test was applied to evaluate qualitative data. A p-value of <0.05 was considered to indicate statistical significance. The 95% confidence intervals were also determined.

RESULTS

There was no statistically significant difference between the study and control groups with respect to the mean age (59.65[+ or -]10.97 and 58.35[+ or -]4.75 years, respectively) or male:female ratio (14:17 and 14:17, respectively) (Table 1). The demographics of the patients with BPPV and healthy controls are shown in Table 1.

However, the differences between the oxidative parameters in the patient and control groups were statistically significant. At baseline, the patients with BPPV had significantly lower SH levels and SH/TT ratios and significantly higher SS/SH and SS/TT ratios than the healthy controls. After treatment for vertigo, the patients with BPPV still had lower SH levels and SH/TT ratios and significantly higher SS/SH and SS/TT ratios than the healthy controls (Figure 1, 2) (Table 2).

DISCUSSION

To the best of our knowledge, this is the first study to investigate SH/SS homeostasis as a novel marker of oxidative stress in patients with BPPV. Specifically, we examined SH/SS homeostasis in a study group of patients with BPPV and a control group of healthy individuals. Our study showed a significant difference between the groups.

Free oxygen radicals are naturally generated during every reaction in the body. Normally, these unstable electron-laden chemicals are largely destroyed or removed by the body's natural antioxidant defense systems. Oxidative stress occurs due to an inadequate response to the formation of free radicals. Among the major non-enzymatic antioxidants able to eliminate oxidative stress in the cell are sulfhydryl group (-SH) containing SHs [11]. Circulating blood albumin binds SH groups via albumin cysteine residues. Reversible SS bonds form with cysteine residues located at the active sites of the protein, thereby reducing the toxicity of ROS [12, 13]. TT levels in cells remain constant to ensure continuous SH/SS homeostasis, reflecting the turnover between SSs and SHs [11].

A global plasma oxidative stress index was recently developed and validated in several diseases. Oxidative stress is related to cardiovascular diseases and their risk factors, such as diabetes, hypertension, and obesity, all of which are highly prevalent in numerous countries all over the world. A relationship between a decline in thiol levels and several systemic diseases has been demonstrated in many studies [14, 15]. While SH/SS homeostasis could previously only be measured by measuring the levels of individual components, Erel and Neselioglu [11] described a new method that allows for the measurement of the levels of these compounds both individually and cumulatively.

Episodes of dizziness are common in the elderly and significantly increase the risk of falls. The incidence of BPPV increases with age. Peripheral vestibular dysfunction, including BPPV, is one of the most common causes of dizziness among the elderly and is one of the most frequent diseases seen in dizziness clinics. The results of our study revealed that oxidative stress may be one of the etiologic factors in the development of BPPV. However, in our study, while SH and TH levels were significantly lower in the study group than in the control group, SS levels did not significantly differ between the groups. Patients with BPPV were treated with Epley's repositioning maneuver and then followed up (outpatient visits) until nystagmus was resolved. SH and TH levels increased after treatment, but this was not statistically significant. The SS/SH, SS/TT, and SH/TT ratios, which represent corrected values and predominantly indicate oxidative homeostasis, significantly differed between the control and study groups: the patients with BPPV had significantly higher SS/SH and SS/TT ratios and lower SH/TT ratios. These findings are consistent with an increase in oxidative stress in patients with BPPV. However, there was no significant difference in oxidative stress parameters in the patients with BPPV before treatment versus after treatment. Although oxidative stress may play a role in the development of BPPV, the increase in oxidative stress did not respond to the treatments that we administered to our patients. Gucluturk et al. [16] studied the levels of the antioxidant paraoxonase in patients with BPPV before and after treatment and reported results similar to ours.

In the semicircular canals, the presence of otoconial debris originating from the utriculus and sacculus causes BPPV when the head position is changed quickly and suddenly [2]. Otoconia consist of calcite, a mixture of calcium and carbonates. The structure of otoconia differs from that of teeth and bones because of its carbonate, rather than phosphate, composition. In the inner ear, calcium and carbonate levels are under the control of the calcium channel transport system. Normal levels of calcium and carbonate are important for the maintenance of otoconial function. Vibert et al. [17] designed an osteoporosis model in ovariectomized rats and studied the ultrastructure of their otoconia. The otoconia of these rats were less dense and contained less calcium; similar mechanisms may underlie the pathogenesis of BPPV. Talaat et al. [18] found that patients with recurrent or non-recurrent BPPV had significantly lower levels of vitamin D than control patients, while patients with recurrent BPPV had significantly lower vitamin D levels than those with non-recurrent BPPV. In another study, Talaat et al. [19] found a relationship between the recovery of serum 25-hydroxyvitamin D3 levels and a significant reduction in the rate of BPPV relapse.

Oxidative stress is related to calcium metabolism, with the endoplasmic reticulum being the most important cellular site for calcium storage and protein folding. In the presence of cell stress, the endoplasmic reticulum may initiate an increase in cellular calcium levels, causing rupture of the mitochondrial membrane and apoptosis.

Recent studies have suggested that oxidative stress and inner ear diseases are related. Brosel et al. [20] reported a strong link between oxidative stress, the related apoptosis of cochlear cells, and age-related hearing loss. Dinc et al. [11] found significant differences in SH/SS homeostasis between patients with sudden sensorineural hearing loss and control patients. Tsai et al. [21] reported increased levels of oxidative stress markers in blood samples from patients with BPPV.

Iwasaki and Yamasoba. [22] showed a correlation between age-related decrease in vestibular function and age-related decline in vestibular hair cells and neurons. The underlying mechanism of age-related cell loss in the vestibular end organ is not known, but the cumulative effect of a genetic predisposition and oxidative stress may play an important role. They recommended conducted further studies on the protective effect of antioxidant therapies with respect to vestibular function during aging.

These studies, together with the present results, indicate a role of oxidative stress in the development of BPPV, through both calcium metabolism and the direct toxic effects of free oxygen radicals, which trigger apoptosis. These mechanisms may also have a synergistic effect.

CONCLUSION

Our study was conducted on a small patient group. Further studies with larger samples are needed to evaluate and compare other oxidative stress markers, such as paraoxonase and arylesterase, as well as the total antioxidant status in patients with BPPV.

Ethics Committee Approval: Ethic committee approval was received for this study from Ethics Committee of Umraniye Training and Research Hospital (Decision No: 2017/76).

Informed Consent: Written informed consent was obtained from the patients who participated in this study.

Peer-review: Externally peer-reviewed.

Author Contributions: Concept - E.S.; Design - E.S.; M.E.D.; Supervision - E.S.; Resource - E.S.; Materials - E.S., C.B., O.E.; Data Collection and/or Processing - B.Y.O.; Analysis and/or Interpretation - C.B., O.E.; Literature Search - I.D.; Writing - I.D.; Critical Reviews - E.S., M.E.D., I.D.

Conflict of Interest: No conflict of interest was declared by the authors.

Financial Disclosure: The authors declared that this study has received no financial support.

REFERENCES

[1.] Liu Y, Wang W, Zhang AB, Bai X, Zhang S. Epley and Semont maneuvers for posterior canal benign paroxysmal positional vertigo: A network meta-analysis. Laryngoscope 2016; 126: 951-5. [CrossRef]

[2.] Fay JL. Benign Paroxysmal Positional Vertigo in 2 Children: A Case Series. Pediatr Phys Ther 2016; 28: 355-60. [CrossRef]

[3.] Hall SF, Ruby RR, McClure JA. The mechanics of benign paroxysmal vertigo. J Otolaryngol 1979; 8: 151-8.

[4.] Schuknecht HF. Cupulolthiasis. Arch Otolaryngology 1969; 70: 765-78. [CrossRef]

[5.] Das S, Rea PA. Bilateral posterior semi-circular canal obliteration surgery for refractory benign paroxysmal positional vertigo (BPPV) in three patients. Clin Otolaryngol 2017; 42: 480-3. [CrossRef]

[6.] Crimi E, Ignarro LJ, Napoli C. Microcirculation and oxidative stress. Free Radic Res 2007; 41: 1364-75. [CrossRef]

[7.] Gucluturk MT, Unal ZN, Ismi O, Cimen MB, Unal M. The Role of Oxidative Stress and Inflammatory Mediators in Benign Paroxysmal Positional Vertigo. J Int Adv Otol 2016; 12: 101-5. [CrossRef]

[8.] Unal M, Tamer L, Pata YS, Kilic S, Degirmenci U, Akbas Y, et al. Serum levels of antioxidant vitamins, copper, zinc and magnesium in children with chronic rhinosinusitis. J Trace Elem Med Biol 2004; 18: 189-92. [CrossRef]

[9.] Dogruer ZN, Unal M, Eskandari G, Pata YS, Akbas Y, Cevik T, et al. Malondialdehyde and antioxidant enzymes in children with obstructive adenotonsillar hypertrophy. Clin Biochem 2004; 37: 718-21. [CrossRef]

[10.] Ulusoy S, Ayan NN, Dinc ME, Is A, Bicer C, Erel O. A new oxidative stress marker for thiol-disulphide homeostasis in seasonal allergic rhinitis. Am J Rhinol Allergy 2016; 30: 53-7. [CrossRef]

[11.] Dinc ME, Ulusoy S, Is A, Ayan NN, Avincsal MO, Bicer C, et al. Thiol/disulphide homeostasis as a novel indicator of oxidative stress in sudden sensorineural hearing loss. J Laryngol Otol 2016; 130: 447-52. [CrossRef]

[12.] Erel O, Neselioglu S. A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem 2014; 47: 326-32. [CrossRef]

[13.] Winterbourn CC. Reconciling the chemistry and biology of reactive oxygen species. Nat Chem Biol 2008; 4: 278-86. [CrossRef]

[14.] Thomas JA, Poland B, Honzatko R. Protein sulfhydryls and their role in the antioxidant function of protein S-thiolation. Arch Biochem Biophys 1995; 10: 319: 1-9. [CrossRef]

[15.] Kundi H, Ates I, Kiziltunc E, Cetin M, Cicekcioglu H, Neselioglu S, et al. A novel oxidative stress marker in acute myocardial infarction; thiol/disulphide homeostasis. Am J Emerg Med 2015; 33: 1567-71. [CrossRef]

[16.] Gucluturk MT, Unal ZN, Ismi O, Cimen MB, Unal M. The Role of Oxidative Stress and Inflammatory Mediators in Benign Paroxysmal Positional Vertigo. J Int Adv Otol 2016; 12: 101-5. [CrossRef]

[17.] Vibert D, Sans A, Kompis M, Travo C, Muhlbauer RC, Tschudi I, et al. Ultrastructural changes in otoconia of osteoporotic rats. Audiol Neurootol 2008; 13: 293-301. [CrossRef]

[18.] Talaat HS, Abuhadied G, Talaat AS, Abdelaal MS. Low bone mineral density and vitamin D deficiency in patients with benign positional paroxysmal vertigo. Eur Arch Otorhinolaryngol 2015; 272: 2249-53. [CrossRef]

[19.] Talaat HS, Kabel AM, Khaliel LH, Abuhadied G, El-Naga HA, Talaat AS. Reduction of recurrence rate of benign paroxysmal positional vertigo by treatment of severe vitamin D deficiency. Auris Nasus Larynx 2016; 43:237-41. [CrossRef]

[20.] Brosel S, Laub C, Averdam A, Bender A, Elstner M. Molecular aging of the mammalian vestibular system. Ageing Res Rev 2016; 26: 72-80. [CrossRef]

[21.] Tsai KL, Cheng YY, Leu HB, Lee YY, Chen TJ, Liu DH, et al. Investigating the role of Sirt1-modulated oxidative stress in relation to benign paroxysmal positional vertigo and Parkinson's disease. Neurobiol Aging 2015; 36: 2607-16. [CrossRef]

[22.] Iwasaki S, Yamasoba T. Dizziness and Imbalance in the Elderly: Age-related Decline in the Vestibular System. Aging Dis 2014; 6: 38-47. [CrossRef]

Ethem Sahin [iD], Ildem Deveci [iD], Mehmet Emre Dinc [iD], Berna Yayla Ozker [iD], Cemile Bicer [iD], Ozcan Erel [iD]

Department of Otolaryngology, Head and Neck Surgery, Bayindir Hospital Icerenkoy, Istanbul, Turkey (ES, BYO)

Department of Otolaryngology, Head and Neck Surgery, Umraniye Training and Research Hospital, Istanbul, Turkey (ID)

Department of Otolaryngology, Istanbul Okmeydani Training and Research Hospital, Istanbul, Turkey (MED)

Department of Medical Biochemistry, Diskapi Yildirim Beyazit Training and Resarch Hospital, Ankara, Turkey (CB, OE)

ORCID IDs of the authors: E.S. 0000-0003-4593-0968; I.D. 0000-0002-1573-8345; M.E.D. 0000-0002-9931-8961; B.Y.O. 0000-0001-7267-6550; C.B. 0000-0001-7937-4475; O.E. 0000-0002-2996-3236

Cite this article as: Sahin E, Deveci I, Dinc ME, Yayla Ozker B, Bicer C, Erel O. Oxidative status in patients with benign paroxysmal positional vertigo. J Int Adv Otol 2018; 14(2): 299-303.

Corresponding Address: Ethem Sahin E-mail: esahin@bayindirhastanesi.com.tr

Submitted: 23.11.2017 * Accepted: 12.12.2017 * Available Online Date: 16.03.2018

DOI: 10.5152/iao.2018.4756
Table 1. Demographic parameters

                               Total              Control group (n=31)

Age (years)    Min-Max         34-78              51-67
               Mean[+ or -]SD  59.00[+ or -]8.41  58.35[+ or -]4.75
Gender; n (%)  Female          34 (54.8)          17 (54.8)
               Male            28 (45.2)          14 (45.2)

               Patient group (n=31)  p

Age (years)    34-78                 (a) 0.551
               59.65[+ or -]10.97
Gender; n (%)  17 (54.8)             (b) 0.999
               14 (45.2)

(a) Independent samples t-test
(b) Pearson's chi-squared test
SD: Standard deviation

Table 2. Serum native thiol/disulfide and total thiol levels

                          (0) Control group    (1) Patient group
                                                   (at admission)

SH       Min-Max          280.30-454.80        128.60-395.30
         Mean[+ or -]SD   375.95[+ or -]38.95  305.22[+ or -]59.70
TT       Min-Max          329.80-514.50        162.27-456.51
         Mean[+ or -]SD   417.98[+ or -]40.92  347.03[+ or -]66.03
SS       Min-Max           15.70-34.45           1.10-38.71
         Mean[+ or -]SD    21.01[+ or -]4.87    20.91[+ or -]9.77
SS/SH    Min-Max            4.02-10.78           0.45-18.97
         Mean[+ or -]SD     5.76[+ or -]1.72    10.23[+ or -]4.81
SS/TT    Min-Max            3.72-8.87            0.44-13.75
         Mean[+ or -]SD     5.12[+ or -]1.32     8.23[+ or -]3.42
SH/TT %  Min-Max           82.26-92.56          72.49-99.11
         Mean[+ or -]SD    89.75[+ or -]2.65    83.54[+ or -]6.84

         (2) Patient group      (a) p (0-1)   (a) p (0-2)  (c) p (1-2)
             (after treatment)

SH       218.90-394.20          <0.001 (**)   <0.001 (**)  0.751
         308.60[+ or -]45.08
TT       248.68-451.32          <0.001 (**)   <0.001 (**)  0.620
         352.88[+ or -]47.90
SS         7.97-36.71            0.956         0.504       0.545
          22.14[+ or -]7.96
SS/SH      3.52-22.32           <0.001 (**)   <0.001 (**)  0.671
          10.67[+ or -]4.45
SS/TT      3.29-15.43           <0.001 (**)   <0.001 (**)  0.630
           8.59[+ or -]2.91
SH/TT %   69.14-93.42           <0.001 (**)   <0.001 (**)  0.630
          82.83[+ or -]5.83

(a) Independent samples t-test
(c) Paired samples t-test
(**) p<0.01
SD: standard deviation; SH: native thiol; TT: total thiol; SS: disulfide
COPYRIGHT 2018 AVES
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2018 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Original Article
Author:Sahin, Ethem; Deveci, Ildem; Dinc, Mehmet Emre; Ozker, Berna Yayla; Bicer, Cemile; Erel, Ozcan
Publication:The Journal of the International Advanced Otology
Article Type:Clinical report
Date:Aug 1, 2018
Words:3269
Previous Article:Evaluation of Benign Paroxysmal Positional Vertigo in American Football Players.
Next Article:The Reliability and Validity of "Dokuz Eylul University Meniere's Disease Disability Scale".
Topics:

Terms of use | Privacy policy | Copyright © 2020 Farlex, Inc. | Feedback | For webmasters