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Outbreak of cyclosporiasis associated with imported raspberries, Philadelphia, Pennsylvania, 2000. (Research).

An outbreak of cyclosporiasis occurred in attendees of a wedding reception held in Philadelphia, Pennsylvania, on June 10, 2000. In a retrospective cohort study, 54 (68.4%) of the 79 interviewed guests and members of the wedding party met the case definition. The wedding cake, which had a cream filling that included raspberries, was the food item most strongly associated with illness (multivariate relative risk, 5.9; 95% confidence interval, 3.6 to 10.5). Leftover cake was positive for Cyclospora DNA by polymerase chain reaction analyses. Sequencing of the amplified fragments confirmed that the organism was Cyclospora cayetanensis. The year 2000 was the fifth year since 1995 that outbreaks of cyclosporiasis definitely or probably associated with Guatemalan raspberries have occurred in the spring in North America. Additionally, this is the second documented U.S. outbreak, and the first associated with raspberries, for which Cyclospora has been detected in the epidemiologically implicated food item.


On June 10, 2000, a total of 83 persons attended a catered wedding reception in Pennsylvania. Approximately 8 days later, the bride notified the local health department that she, her husband, and many guests at the reception had a gastrointestinal illness. Stool specimens from attendees were positive for oocysts of the coccidian parasite Cyclospora cayetanensis, and an epidemiologic investigation was begun by the Philadelphia Department of Public Health and the Centers for Disease Control and Prevention (CDC).

Previous foodborne outbreaks of cyclosporiasis in the United States have been associated with consumption of fresh raspberries, mesclun lettuce, and basil (1-8). An important feature of the biology of Cyclospora is that oocysts excreted in feces require days to weeks outside the host to sporulate and thus to become infectious. However, the minimum time required for sporulation is unknown. Cyclospora oocysts must survive in the environment long enough to sporulate and infect a susceptible host, which suggests that they are very hardy.


Epidemiologic Investigation

A retrospective cohort study was conducted by administering a structured questionnaire by telephone about symptoms and event-related exposures. A case of cyclosporiasis was defined as onset of illness 1-14 days after the reception and either (a) one positive stool specimen and at least one gastrointestinal symptom (i.e., loose stools, nausea, vomiting, stomach cramps, gas/bloating, loss of appetite, and unintentional weight loss) or constitutional symptom (i.e., fever, chills, muscle aches, generalized body aches, and headaches); (b) any loose stools in a 24-hour period and at least one other gastrointestinal or constitutional symptom; or (c) a total of at least three gastrointestinal symptoms.

We attempted to identify more cases of cyclosporiasis by having the Pennsylvania State Bureau of Epidemiology alert health departments throughout the state of the investigation. We also requested the catering company to contact the six groups to which they had served raspberries during the period June 2-4 that might have been from the suspected raspberry shipments.

Statistical Analyses

Univariate relative risks and 95% confidence intervals (CIs) were calculated by using Epi-Info, version 6.04 (CDC). Multivariate Poisson regression analyses were conducted by using SAS, version 8.0 for Windows (SAS Institute Inc., Cary, NC).

Laboratory Investigation

The local diagnostic reference laboratory examined stool specimens from five patients for Cyclospora oocysts by Kinyoun's modified acid-fast technique. CDC reevaluated specimens from three of these patients either by examining slides made by the local laboratory or by UV fluorescence microscopy of a stool specimen concentrated by the formalin-ethyl acetate technique. An unknown number of specimens were tested at the local diagnostic reference laboratory for Cryptosporidium parvum; Salmonella, Shigella, and Campylobacter spp.; and Escherichia coli O157:H7.

CDC and the Food and Drug Administration (FDA) used molecular methods to examine leftover wedding cake, which the bride and groom had frozen, for Cyclospora. FDA's methods were as follows. One-gram portions of the icing from the top of the wedding cake, the raspberry filling, and the cake itself were processed for molecular analyses by using 20 mL of Tris buffer (containing 100 mM Tris-HCL, pH 8.0, 10 mM EDTA, 150 mM NaCl) and 5 mL of ethyl acetate. The samples were shaken vigorously, heated at 56[degrees]C, and gently agitated for 30 min. The samples were centrifuged for 15 min at 4500 x g at 15[degrees]C. The organic phase, the interface, and the aqueous (upper) phase were aspirated. Next, the pellet was washed twice with 25 mL of Tris buffer and centrifuged as above. The pellet was suspended in 4 mL of Tris buffer, layered over a 4-mL Optiprep solution (Invitrogen Corporation, Carlsbad, CA), and diluted 1:1 with the Tris buffer. The samples were centrifuged for 15 min at 250 x g at 15[degrees]C. The aqueous phase, the interface, and 1.5 mL of the upper portion of the Optiprep layer were removed and saved in a 15-mL centrifuge tube. The remaining pellet and Optiprep solution were discarded. All saved samples were diluted further with 2-3 mL of Tris buffer and centrifuged for 10 min at 20,000 x g.

The supernatant from each sample was aspirated to within 1 mL of the bottom, and the pellets were suspended in this volume and transferred to a 1.5-mL microcentrifuge tube. The samples were then centrifuged for 2 min in a microcentrifuge at 14,000 x g, and the supernatant was aspirated. The final pellets were suspended in 50 [micro]L of distilled water, and a 10-[micro]L volume of each sample was spotted onto Flinders Technologies of Australia (FTA) filters (Fitzco, Inc., Maple Plain, MN) for DNA extraction and subsequent polymerase chain reaction (PCR) analyses as described previously (9).

CDC's methods were as follows. DNA was extracted directly from nine randomly selected 1-g portions of raspberry filling from the cake by using selected components from the FastDNA kit (BIO 101, Inc., Vista, CA). The portions were placed into tubes containing disruption beads that were included in the kit, and DNA extraction was performed as described (10).

Nested PCR amplification was performed by using primers modified from those described elsewhere (11). The restriction sites EcoRI and BamHI were removed from the 5' end, and bases matching the C. cayetanensis small subunit (SSU) rRNA coding region were added. Thus, primers CYCF1 (5'-ATTACCCAATGAAAACAGTTT-3') and CYCR2 (5'-TGCAGGAGAAGCCAAGGTAGG-3') were used for the first step of nested amplification; the preamplified fragment was nested with primers CYCF3 (5'-GCCTTCCGCGCTTCGCTGCGT-3') and CYCR4 (5'- TCGTCTTCAAACCCCCTACTG-3'). Amplification with these primers generates a 294-bp fragment from the C. cayetanensis SSUrRNA coding region.

Besides the nested PCR, a 636-bp fragment from the Cyclospora sp. SSUrRNA, flanked by primers CYCF1/ CYCR2, was amplified and sequenced to identify the parasite at the species level. This fragment contains substitutions that allow differentiation among the Cyclospora spp. for which the sequence of the SSUrRNA coding region is available in GenBank (i.e., C. cayetanensis, C. cercopitheci, C. colobi, and C. papionis). Sequencing reactions were performed on PCR products that had been purified with the StrataPrep PCR Purification Kit (Stratagene, La Jolla, CA). Sequencing reactions, analyses, and assemblage of sequences were performed as described (12).

Environmental and Source Investigations

The reception facility was inspected, and food-handling and storage practices were observed. Information about illness in the catering staff was collected by examining the caterer's sick logs. The caterer was also asked to contact the health department if staff called in sick. Ill staff members would then be asked whether they had had a gastrointestinal illness during the 3 weeks before and after the reception.

The caterer and the distributors that supplied his company were interviewed, and shipping documents were obtained to determine the source of the raspberries used in the cake (13). In cooperation with the Guatemalan government, FDA inspected one of the farms that could have supplied the raspberries served at the wedding; this farm also could have supplied the raspberries for an event in another state in 2000 that was associated with an outbreak of cyclosporiasis.


Epidemiologic Investigation

Seventy-nine (95.2%) of the 83 attendees of the wedding reception were interviewed. Fifty-four (68.4%) of the 79 had illness that met the case definition. Five (9.3%) of the 54 case-patients had laboratory-confirmed cyclosporiasis, and 49 (90.7%) of the 54 case-patients had clinically defined cyclosporiasis. All case-patients who met part (c) of the case definition also met part (b). Therefore, parts (b) and (c) were combined to represent the clinically defined category of the case definition. The median incubation period was 7 days (range 1-9 days) (Figure 1). The symptoms associated with illness are listed in Table 1.


Several food items were significantly associated with illness in univariate analyses (Table 2). However, only the cake, which had a cream filling that included pieces of raspberries, was significantly associated with illness in multivariate analyses (relative risk 5.9; 95% CI 3.6 to 10.5). Mesclun lettuce was used as a garnish for several food items, and fresh basil was served in one food item; neither was significantly associated with illness.

We did not identify additional cases through the Pennsylvania State Bureau of Epidemiology's alert to local health departments. Moreover, the catering company contacted the six groups to which they served raspberries during June 2-4, and no additional cases were identified.

Laboratory Investigation

For three of the five case-patients who had laboratory-confirmed cyclosporiasis (Table 1), stool specimens were sent to CDC and reconfirmed as positive for Cyclospora oocysts. Results of stool testing for other microbes were negative.

PCR analyses conducted by both FDA and CDC confirmed the presence of Cyclospora DNA in the raspberry filling of the cake. At FDA, an FTA-based PCR identified Cyclospora DNA in the raspberry filling but not in the icing from the top of the cake or in the cake itself. At CDC, PCR amplification was possible from DNA directly extracted from four of the nine portions of the raspberry filling that were tested. All positive samples generated sequences that were identical to C. cayetanensis in the 636-bp PCR product.

Environmental and Source Investigations

None of the catering staff reported having been ill during the 3 weeks before or after the reception. However, on July 18, the local health department learned, through routine surveillance, about a caterer, who had worked during the reception, in whom gastrointestinal illness developed 8 days later; she had laboratory-confirmed cyclosporiasis. Although she could not recall her duties for the event, her usual tasks included cutting and serving wedding cake. She also identified two other staff members in whom gastrointestinal illness developed a median of 6 days after the reception; their symptoms resolved spontaneously, and no stool specimens were tested. They had not worked during the reception but had worked later that day and reported that they probably ate leftover cake.

Raspberries were the only produce used in the wedding cake. The catering company had received four shipments of fresh raspberries June 1-3. Some of the raspberries from these shipments were served fresh at events on June 3-4. The leftover raspberries were frozen for 1-4 days. Two to three pints of leftover, unwashed raspberries were thawed, crushed into pieces, and folded into the butter cream filling used in the cake. The cake was then frozen for another 4-5 days until it was thawed and eaten on June 10, the date of the wedding (Figure 2).


Local distributors A and B supplied the four shipments of raspberries received by the catering company during the period June 1-3 (Figure 3). Distributors A and B had received shipments from three other local distributors (C, D, and E) during May 30-June 3. During this period, one Guatemalan farm and one Mexican farm were the sources of the raspberries that were delivered to importers F and G that supplied distributor C, and an unknown number of U.S. farms supplied the raspberries delivered to distributors D and E (Figure 3). Which raspberries from which shipment were actually used in the cake is unknown.


The one Guatemalan farm that could have supplied the raspberries used in the cake was also one of the possible sources (and the only Guatemalan source) of raspberries served at a bridal brunch in Georgia in May 2000 that was associated with an outbreak of cyclosporiasis (14). The Guatemalan farm was the only known source in common to the events in Pennsylvania and Georgia. FDA inspected this farm and found no definitive source for contamination (J. Guzewich, pers. comm).


We investigated an outbreak of cyclosporiasis associated with a wedding reception in Philadelphia in June 2000. The epidemiologic evidence and the identification by PCR analyses of Cyclospora DNA in the food item most strongly associated with illness indicated that the outbreak was foodborne. This is the first outbreak associated with raspberries for which Cyclospora DNA has been identified in the epidemiologically implicated food item. Because of the outbreak, the Philadelphia health department subsequently made cases of Cyclospora infection reportable.

We do not know whether the outbreak involved more cases in Pennsylvania than the cluster of cases we investigated. The catering company for the wedding reception received four shipments of fresh raspberries the weekend before the cake was prepared; which raspberries from which shipment were used for the reception rather than the other six events during the period of interest at which raspberries were served is unknown. We did not identify any more cases of cyclosporiasis by contacting the other six groups. However, perhaps only one of the shipments of raspberries and only some of the raspberries in that shipment were contaminated. The same Guatemalan farm was one of the possible sources of the raspberries (and the only Guatemalan source) served at the reception in Pennsylvania in June 2000 and the bridal brunch in Georgia in May 2000. The identification of only one farm in common to the two events suggests that the events were related.

The year 2000 was the fifth year since 1995 (i.e., 1995, 1996, 1997, 1998, and 2000) that outbreaks of cyclosporiasis occurred in the spring in the United States or Canada that definitely or probably were associated with Guatemalan raspberries (7). However, the recent outbreaks have been much smaller than the multistate outbreaks in 1996 and 1997 (7). After the outbreaks in 1996 and 1997 (2,3), FDA began working with the Guatemalan government and berry industry to improve farming and exporting practices for raspberries. Only farms that meet certain standards--including water, sanitation, and worker hygiene issues--have been allowed to export fresh raspberries to the United States during the "spring season" (March through August). The standards are reviewed and updated yearly. During the spring of 2000, five Guatemalan farms were allowed to export to the United States. After the outbreaks in Pennsylvania and Georgia, FDA did not allow the farm that was in common to the events to export raspberries to the United States during the spring of 2001. No U.S. outbreaks of cyclosporiasis associated with Guatemalan raspberries were identified that spring. During the spring of 2002, only three farms, which have never been implicated in outbreaks of cyclosporiasis, were allowed to export raspberries to the United States.

The cyclosporiasis outbreak in Philadelphia is the first for which raspberries that reportedly had been frozen, rather than served fresh, were implicated. The raspberries used in the wedding cake had been bought fresh but then reportedly frozen and thawed twice before they were served (Figure 2). The temperature of the freezer where the raspberries were stored is unknown. When the freezer was inspected 2 weeks after the outbreak, the temperature of the freezer was -3.3[degrees]C, which might not have been cold enough to kill Cyclospora oocysts. Although limited information is available about the effects of freezing on their survival, for Cryptosporidium parvum, another coccidian parasite, oocysts remain infectious for mice after storage at -10[degrees]C for 1 week (15). Freezing at sufficiently cold temperatures for sufficiently long periods, as during commercial freezing, should kill Cyclospora oocysts. For Cryptosporidium parvum, oocysts were not infectious for mice after storage at -15[degrees]C for 1 week (15).

This Pennsylvania outbreak is the second documented U.S. outbreak, and the first associated with raspberries, for which Cyclospora has been detected in the epidemiologically implicated food item. The first U.S. outbreak for which Cyclospora was detected in a food item occurred in 1999 in Missouri and was associated with fresh basil in a chicken pasta salad (8). Neither the raspberries in 2000 nor the basil in 1999 had been washed. Although all produce should be thoroughly washed before being eaten, other outbreaks (2,3,7) and a laboratory study (16) have shown that washing does not eliminate the risk for Cyclospora infection.

Fortunately, leftovers from both the Pennsylvania and Missouri outbreaks had been frozen and were available for testing. Contamination of the implicated food items for the 1999 outbreak was demonstrated by PCR analyses and light microscopy and for the 2000 outbreak by PCR analyses. The modes of contamination of the produce associated with outbreaks of cyclosporiasis have not yet been definitively identified for any of the outbreaks. However, molecular methods are sensitive tools that can be used to identify the organism in the vehicle and in environmental samples, which then may provide information useful for determining the mode of contamination. Molecular methods for detecting Cyclospora continue to improve (9). Continued research about the biology and epidemiology of Cyclospora is important for identifying effective measures for preventing future outbreaks.
Table 1. Information on attendees of a wedding reception associated
with an outbreak of cyclosporiasis, Pennsylvania, June 2000

 Case-patients Non-case attendees
Variable (n=54) (a) (n=25) (b)

Age, y, median (range) (c) 38.8 (18-87)
Female sex, no. (%) (c) 32 (59.3)
Symptoms, no. (%)
 Diarrhea (d) 54 (100) 1 (4.0)
 Nausea 43 (79.6) 1 (4.0)
 Stomach cramps 41 (75.9) 1 (4.0)
 Chills/sweats 41 (75.9) 0
 Headache 36 (66.7) 0
 Muscle/body aches 36 (66.7) 1 (4.0)
 Fever (e) 32 (59.3) 0
 Vomiting 26 (48.1) 0
 Dizziness 25 (46.3) 0
 Rash 3 (5.6) 0
Hospitalization, no. (%) 2 (3.7) 0
 Length of stay, days, 12 (10-14) 0
 median (range)

(a) Five of the 54 case-patients had laboratory-confirmed

(b) Two of the 25 non-case attendees reported having at least one

(c) Information about age and sex was obtained from only two non-case
attendees and was not included in the table.

(d) Diarrhea was defined as having three or more loose stools in a
24-hour period.

(e) In case-patients, the median temperature was 37.8[degrees]C
(range 37.2-38.5[degrees]C). No non-case attendees were febrile.
Table 2. Food items at a wedding reception that were significantly
associated with cyclosporiasis in univariate analyses, Pennsylvania,
June 2000

 Attack rate in attendees,
 no. ill/no. exposed or
 unexposed (%)

Food items (a) Exposed Unexposed RR (95% CI)

Wedding cake (b) 50/53 (94.3) 4/26 (15.4) 6.1 (2.5 to 15.1)
Fresh fruit (c) 36/43 (83.7) 18/36 (50.0) 1.7 (1.2 to 2.4)
Arugula salad 34/41 (82.9) 20/38 (52.6) 1.6 (1.1 to 2.2)
Focaccia bread 15/16 (93.8) 39/63 (61.9) 1.5 (1.2 to 1.9)
Hearthbaked 23/27 (85.2) 31/52 (59.6) 1.4 (1.1 to 1.9)

(a) Mesclun lettuce was served as a garnish on several hors d'oeuvres
trays, and basil was served fresh in one food item. Neither was
significantly associated with illness.

(b) Food item was statistically significant in multivariate analyses
(relative risk, 5.9; 95% confidence interval [CI] 3.6 to 10.5).

(c) Fresh fruit included strawberries, raspberries, blueberries, and
blackberries. Persons served themselves from a bowl of fresh
strawberries and a bowl of fresh raspberries, blueberries, and
blackberries next to the cake. The raspberries in the bowl came from a
different source than the raspberries in the cake filling and were not
statistically significant in multivariate analyses.


We thank Michael Arrowood, Jeff Bier, Paul Blake, Loretta Brown, Ronald Colucci, Paula Davis-Debella, Phil DeLisle, Michelle Dunaway, Mark Eberhard, Kris Foster, John Guzewich, Travis Hunt, George Jackson, Stephanie Johnston, John Kvenberg, Eva Nace, Jim O'Neal, James Peiffer, Sarah Pichette, Kathleen Sinninger, Luis Solorzano, Bill Tackett, Susanne Wahlquist, Davina Watson, and Kimberly Won for their help with the investigation.


(1.) Koumans EHA, Katz DJ, Malecki JM, Kumar S, Wahlquist SP, Arrowood MJ, et al. An outbreak of cyclosporiasis in Florida in 1995: a harbinger of multistate outbreaks in 1996 and 1997. Am J Trop Med Hyg 1998;59:235-42.

(2.) Herwaldt BL, Ackers ML, Cyclospora Working Group. An outbreak in 1996 of cyclosporiasis associated with imported raspberries. N Engl J Med 1997;336:1548-56.

(3.) Herwaldt BL, Beach MJ, Cyclospora Working Group. The return of Cyclospora in 1997: another outbreak of cyclosporiasis in North America associated with imported raspberries. Ann Intern Med 1999;130:210-20.

(4.) Centers for Disease Control and Prevention. Outbreak of cyclosporiasis--northern Virginia-Washington, D.C.-Baltimore, Maryland, metropolitan area, 1997. MMWR Morb Mortal Wkly Rep 1997;46:689-91.

(5.) Levy DA, Rullan J, Pritchett R, Gossman C, Radke V, Moore J, et al. Multicluster outbreak of foodborne cyclosporiasis associated with fresh basil [abstract no. P-3.19]. In: Program and abstracts of the International Conference on Emerging Infectious Diseases; 1998 Mar 8-11; Atlanta (GA). 1998. p. 86.

(6.) Centers for Disease Control and Prevention. Outbreak of cyclosporiasis--Ontario, Canada, May 1998. MMWR Morb Mortal Wkly Rep 1998;47:806-9.

(7.) Herwaldt BH. Cyclospora cayetanensis: a review, focusing on the outbreaks of cyclosporiasis in the 1990s. Clin Infect Dis 2000;31:1040-57.

(8.) Lopez AS, Dodson DR, Arrowood MJ, Orlandi PA, da Silva AJ, Bier JW, et al. Outbreak of cyclosporiasis associated with basil in Missouri in 1999. Clin Infect Dis 2001;32:1010-7.

(9.) Orlandi PA, Lampel KA. Extraction-free, filter-based template preparation for the rapid and sensitive PCR detection of pathogenic parasitic protozoa. J Clin Microbiol 2000;38:2271-7.

(10.) da Silva AJ, Bornay-Llinares FJ, Moura I, Slemenda SB, Turtle JL, Pieniazek NJ. Fast and reliable extraction of protozoan parasite DNA from fecal specimens. Mol Diagn 1999;4:57-64.

(11.) Relman DA, Schmidt TM, Gajadhar A, Sogin M, Cross J, Yoder K, et al. Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species. J Infect Dis 1996;173:440-5.

(12.) Pieniazek NJ, Bornay-Llinares FJ, Slemenda SB, da Silva AJ, Moura INS, Arrowood MJ, et al. New Cryptosporidium genotypes in HIV-infected persons. Emerg Infect Dis 1999;5:444-9.

(13.) U.S. Food and Drug Administration. Guide to traceback of fresh fruits and vegetables implicated in epidemiological investigations. Rockville (MD): The Division of Emergency and Investigational Operations, Office of Regional Operations, Office of Regulatory Affairs, FDA; 2001. Available from: URL: epigde.html

(14.) Murrow LB, Blake P, Kreckman L. Outbreak of cyclosporiasis in Fulton County, Georgia. Georgia Epidemiology Report January 2002;18:1-2.

(15.) Fayer R, Speer CA, Dubey JP. The general biology of Cryptosporidium. In: Fayer R, editor. Cryptosporidium and cryptosporidiosis. Revised edition of Cryptosporidiosis of man and animals. Boca Raton (FL): CRC Press; 1997. p. 1-33.

(16.) Ortega YR, Roxas CR, Gilman RH, Miller NJ, Cabrera L, Taquiri C, et al. Isolation of Cryptosporidium parvum and Cyclospora cayetanensis from vegetables collected in markets of an endemic region in Peru. Am J Trop Med Hyg 1997:57;683-6.

Alice Y. Ho is the Nurse Epidemiologist for the Philadelphia Department of Public Health, Division of Disease Control. She oversees communicable disease surveillance and outbreak investigations in Philadelphia.

Address for correspondence: Alice Y. Ho, Philadelphia Department of Public Health, Division of Disease Control, 500 South Broad Street, 2nd floor, Philadelphia, PA 19146, USA; fax: 215-545-8362; e-mail:

Alice Y. Ho, * Adriana S. Lopez, ([dagger]) ([double dagger]) Michael G. Eberhart, * Robert Levenson, * Bernard S. Finkel, * Alexandre J. da Silva, ([double dagger]) Jacquelin M. Roberts, ([double dagger]) Palmer A. Orlandi, ([section]) Caroline C. Johnson, * and Barbara L. Herwaldt ([double dagger])

* Philadelphia Department of Public Health, Philadelphia, Pennsylvania, USA; ([dagger]) Atlanta Research and Education Foundation, Atlanta, Georgia, USA; ([double dagger]) Centers for Disease Control and Prevention, Atlanta, Georgia, USA; and ([section]) Food and Drug Administration, Washington, DC, USA
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Author:Herwaldt, Barbara L.
Publication:Emerging Infectious Diseases
Article Type:Statistical Data Included
Geographic Code:1USA
Date:Aug 1, 2002
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