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Osmotic inducement to cryopreserved clones. (Senior Division).

The purpose of my project is to test the influence of dextrose in relationship to osmotic pressure when buffalo grass cultures are frozen in liquid nitrogen. I first placed sterilized cultures in dextrose for 20 minutes and then placed the cultures in PEG. While in PEG I placed the cultures in LN2 until the solution was frozen. I then placed the cultures in a warm water bath and then placed them in test tubes of MS agar and let grow for one month. I also had various controls. I placed cultures in dextrose and PEG by themselves as well as both together with no LN2. I then observed the data. In conclusion, with the aid of dextrose and PEG cloned buffalo grass can be placed in liquid nitrogen to help preserve plant genetic conservation.

Shannon R. George, Woodlin High School.
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Author:George, Shannon R.
Publication:Journal of the Colorado-Wyoming Academy of Science
Article Type:Brief Article
Geographic Code:1USA
Date:Apr 1, 2002
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