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OTOLITH MICROCHEMISTRY OF COASTAL CUTTHROAT TROUT FROM THE MARYS AND WILLAMETTE RIVERS.

Coastal Cutthroat Trout have diverse and complex reproductive and migratory life histories. These include anadromous, adfluvial, fluvial, and resident patterns (Trotter 1989; Goodson 2008; Pearcy and others in press). Cutthroat Trout are found in all tributaries of the Willamette River (Dimick and Merryfield 1945), where they have 2 major life-history patterns: resident fish that live in headwater tributaries of the Willamette River for their entire lives, and fluvial fish that migrate and spawn in lower-gradient tributaries of the Willamette River in the winter and spring, but live most of their lives in the mainstem of the river, where they grow to larger sizes than the resident fish (Moring and others undated; Nicholas 1978; Ecosystems Northwest 1989; Hooton 1997).

Otolith chemistry, including both elemental and isotopic composition, and otolith structure may help to differentiate these life-history patterns as well as identify migrations and habitat use during early life histories. Otolith chemistry has been used to distinguish migratory patterns of other salmonid fishes, including Cutthroat Trout within a watershed or basin (Klaue and others 2002; Wells and others 2003; Zydlewski and others 2009; Muhfield and others 2012), but no studies, to our knowledge, have attempted to distinguish resident from fluvial fish.

The basic question in this preliminary study is, can we use water chemistry and otolith chemistry to distinguish resident trout from those whose mothers reside most of the year in the Willamette River and then migrate into the Marys River? If so, this would help differentiate the relative abundance and habitats of these life histories, so that restoration, monitoring, and management of these different life-history types could be prioritized.

METHODS

We took water samples for analysis from the Marys River and 5 of its tributaries in March and May 2012 and collected 2 samples from the Willamette River in March and 1 in August 2012 (black dots in Fig. 1, Table 1). Water samples were filtered and analyzed for [.sup.18][delta]O at Oregon State University's (OSU) Stable Isotope Laboratory (http://stable-isotope.coas. oregonstate.edu). Water calcium (Ca), strontium (Sr), and barium (Ba) were quantified with Inductively Coupled Plasma Optical Emission Spectroscopy (317.933 nm for Ca, 421.552 nm for Sr, and 455.403 for Ba). Accuracy was assessed using National Institute of Standards and Technology Standard Reference Material (NIST SRM) 1643: n = 3, Ca = 100% [+ or -] 0.003, Ba = 100% [+ or -] 0.006, and Sr= 100% [+ or -] 0.01. Water [.sup.87]Sr/[.sup.86]Sr was measured using a NuPlasma multi-collector inductively coupled plasma mass spectrometer (MC-ICPMS). Accuracy was assessed by comparison with NIST SRM 987 ([.sup.87]Sr/[.sup.86]Sr = 0.71034) and was 99.98% with an internal precision of [+ or -] 0.000019 ppm (n =10).

We collected juvenile fish by seining in 5 tributaries of the Marys River in May 2012, at the same location where we sampled water, and collected adults at Willamette River RM 151 near Harrisburg, Oregon, in August 2012. Sagittae were removed, acid washed, cleaned, dried, embedded in resin, ground, and polished using the methods described in Miller (2011). Three analytes ([.sup.43]Ca, [.sup.86]Sr, and [.sup.138]Ba) were measured to quantify calcium, strontium, and barium using a Thermo X-series II spectrometer and Photon Machines Analyte G2 laser. The laser was set at a pulse rate of 7 Hz with a 50-|.tm ablation spot size and traveled at 7 [micro]m/s. Life-history transects were collected across the otoliths perpendicular to growth rings to generate time series. Normalized analytes (ion ratios) were converted to molar ratios using standard procedures (Kent and Ungerer 2006; Miller 2009). Instrument precision (mean percent relative standard deviation) was 4.5% for Ca, 4.7% for Sr, and 5.2% for Ba across all samples and days (n = 50), and accuracy for Sr/Ca was 103 [+ or -] 8 % and 111[+ or -] 9 % for Ba/Ca (n = 5) based on USGS MACS-1 and MACS-3.

To quantify otolith [.sup.87]Sr/[.sup.86]Sr, we used a NuPlasma MC-ICPMS and Photon Machines Analyte G2 laser. We used the methods of Woodhead and others (2005) as described in Miller and Kent (2009) to correct for Kr and Rb interferences and monitor for Ca argide/dimer formation. The laser was set at a pulse rate of 10 Hz with a 40-[micro]m ablation spot size and travelled at 5 [micro]m/sec. To assess instrument accuracy, we determined the [.sup.87]Sr/[.sup.86]Sr of a marine gastropod (= 0.70918) and consistently obtained a mean value of 0.70902 [+ or -] 0.000099 2 SE (n = 7); otolith [.sup.87]Sr/[.sup.86]Sr values were corrected for this difference. All elemental and isotopic analyses were completed at OSU's WM Keck Collaboratory for Plasma Mass Spectrometry (http://wmkeck-icpms.coas.oregonstate.edu/). We determined average elemental and isotopic values for the core (100 to 200 microns centered within the otolith core) and edge (50 to 100 microns at the outermost otolith edge) regions for each fish.

RESULTS

Is the water chemistry different between the tributaries of the Marys River and the main stem of the Willamette River?

The answer is "yes", based on lower values of [delta][.sup.18]O and [.sup.87]Sr/[.sup.86]Sr and higher Sr/Ca ratios in Willamette River water than most tributaries of the Marys River (Table 1). [delta][.sup.18]O is a measure of the ratio of [.sup.18]O to [.sup.16]O. As water vapor in the atmosphere moves from the coast to the Cascade Range, it becomes depleted in the heavier isotope or more negative (Rayleigh Fractionation, see Arag uas-Araguas and others 2000). Hence, the lower values of [delta][.sup.18]O are expected in precipitation falling over tributaries east of the mainstem Willamette River than tributaries west of the river. Plots of both water Sr/Ca versus Ba/Ca and water [.sup.87]Sr/[.sup.86]Sr versus Sr/Ca show strong positive relationships during both March and May (Fig. 2). The Sr/Ca ratios in the Willamette River were very similar during March (3.15, 3.16 mM/M) and August 2012 (3.1 mM/M, Table 1), suggesting little seasonal change in this ratio.

Overall, there was overlap in Sr/Ca and Ba/Ca in the Willamette and Marys rivers (Table 1). The Willamette River had moderate to low levels of Ba/Ca and moderate levels of Sr/Ca compared to some sites in the Marys River and its tributaries. Water samples in headwaters of the Tumtum and Marys rivers at Blodgett also had high Ba/Ca and Sr/Ca ratios, water chemistries different from other tributaries and the mainstem Willamette River. Water [.sup.87]Sr/[.sup.86]Sr was consistently lower in the Willamette River (mean = 0.70400 [+ or -] 0.00017 SD; 95% CI = 0.70377 to 0.70423) than in the Marys River (mean = 0.70639 [+ or -] 0.00141 SD; 95% CI = 0.70526 to 0.707517) (Table 1). Additional data on the Willamette River show that water [.sup.87]Sr/[.sup.86]Sr is consistently [less than or equal to]0.70450 (Bourret 2013), and no Marys River water sample was <0.70450.

How does the microchemistry of cutthroat otoliths compare with that of the water where they were caught?

Elemental data were collected from the otoliths of 10 juvenile Cutthroat Trout collected from tributaries of the Marys River and from 6 adult fish from the Willamette River at Harris-burg. Strontium isotopic data were collected from 6 of the 10 juveniles and 5 of the 6 adults (Table 2). The elemental and isotopic composition of otolith edges, which is the product of recent growth, was expected to be the most similar to those ratios in the water when these fish were collected.

Sr/Ca (mM/M) and Ba/Ca ([micro]M/M) were always much higher for proximate water than for the otolith edges (Table 3), which is expected because of elemental partitioning; hence otolith elemental ratios are typically a fraction of ambient water (Campana 1999; Miller 2011). The otolith edge chemistry (Sr/Ca and Ba/Ca) of the juveniles was positively correlated with water chemistry (r = 0.937, n = 8, P = 0.001 for Sr/Ca, and r = 0.872, n = 8, P= 0.005 for Ba/Ca), although the relationships were primarily driven by the high Sr/Ca and Ba/Ca at 1 site (Tumtum at Blodgett) (Tables 1 and 3). Otolith edge [.sup.87]Sr/[.sup.86]Sr was also positively correlated with water [.sup.87]Sr/[.sup.86]Sr (r = 0.740, n = 11, P < 0.01). However, unlike elemental incorporation, [.sup.87]Sr/[.sup.86]Sr is incorporated into the otolith with no partitioning (Kennedy and others 2000; Weber and others 2005) (Tables 2 and 3).

Are values for the edges versus the core of the otoliths similar, or do they exhibit changes over time, suggesting maternal effects, changes in distribution, or differential incorporation?

For juveniles, there was extensive variability in otolith chemistry within both the core and edge signatures (Tables 2 and 3). Mean core Sr/Ca ranged from 0.38 [+ or -] 0.03 SD to 2.02 [+ or -] 0.24 SD mM/M, and mean edge Sr/Ca ranged from 0.36 [+ or -] 0.02 SD to 2.07 [+ or -] 0.18 SD mM/M. Juvenile core Ba/Ca ranged from a mean 2.7 [+ or -] 0.36 SD to 43.8 [+ or -] 5.80 SD uM/M, and edge Ba/Ca ranged from a mean of 1.0 [+ or -] 0.34 SD to 28.2 [+ or -] 2.71 SD [micro]M/M (Table 2). Juvenile core [.sup.87]Sr/[.sup.86]Sr values were also variable, ranging from a mean of 0.70366 [+ or -] 0.00036 SD to 0.70612 [+ or -] 0.00046 SD. Juvenile edge [.sup.87]Sr/[.sup.86]Sr values ranged from 0.70428 [+ or -] 0.00133 SD to 0.70590 [+ or -] 0.00028 SD (Table 3).

For adults, there was also extensive variability in otolith chemistry within both the core and edge signatures (Tables 2 and 3). Core Sr/Ca ranged from 0.20 [+ or -] 0.04 SD to 0.76 [+ or -] 0.07 SD mM/M, and the edge Sr/Ca ranged from 0.65 [+ or -] 0.05 SD to 0.72 [+ or -] 0.06 SD mM/M. Core Ba/Ca of adults ranged from 0.9 [+ or -] 0.20 SD to 9.0 [+ or -] 1.35 SD [micro]M/M in adult otoliths, and the edge Ba/Ca ranged from 1.9 [+ or -] 0.17 SD to 6.8 [+ or -] 2.38 SD [micro]M/M (Table 2). Adult core [.sup.87]Sr/[.sup.86]Sr values were also variable, ranging from a mean of 0.70387 [+ or -] 0.00036 SD to 0.70733 [+ or -] 0.00029 SD. However, adult edge [.sup.87]Sr/[.sup.86]Sr values were similar (0.70365 [+ or -] 0.00019 SD to 0.70396 [+ or -] 0.00015 SD) (Table 3).

Several juveniles (fish 3, 4, and 7) had relatively low otolith core values of [.sup.87]Sr/[.sup.86]Sr (mean = 0.70366 to 0.70408), which fell within the 95% CI for Willamette River water (0.70377 to 0.70423) (see Fig. 3 for example). Core chemistry reflects the maternal environment during egg incubation (Volk and others 2000; Zimmerman and Reeves 2002; Miller and Kent 2009). Therefore, given that Marys River water (mean = 0.70639 [+ or -] 0.00141 SD) and juvenile otolith edge [.sup.87]Sr/[.sup.86]Sr (mean = 0.70512 [+ or -] 0.00060 SD) are consistently greater than the core [.sup.87]Sr/[.sup.86]Sr values, those juveniles with lower core [.sup.87]Sr/[.sup.86]Sr likely originated in the Willamette River or an area with similar water chemistry, or are the offspring of Willamette River mothers or mothers that completed oogenesis in a location with water [.sup.87]Sr/[.sup.86]Sr similar to the Willamette River.

For adults, based on the relatively large differences between core and edge Sr/Ca (0.20 versus 0.72 mM/M) or Ba/Ca (8.0 versus 2.0 [micro]M/M), it appears that fish 1, 3, and 5 moved to a chemically distinct habitat after early rearing. Similarly, these fish, based on the relatively high core [.sup.87]Sr/[.sup.86]Sr values ([greater than or equal to]0.70460), likely reared in a tributary before moving into the Willamette River later in life.

Does the chemistry of otoliths of "resident" fish caught above the Rock Creek dam differ from fish that may be "fluvial" below the dam?

Otolith edge values of fish from Rock Creek 1 above the dam, where fish cannot make downstream migrations, were higher for fish 1, 2, and 5 (Ba/Ca ratios of 5.7 to 8.3 [micro]M/M) than fish 3 and 4 below the dam (Ba/Ca ratios of 0.0 to 1.7 [micro]M/M) (Table 2, Fig. 4, Fig. 5). This reflects the higher Ba/Ca ratios in the water above the dam compared to water below the dam.

The highest core [.sup.87]Sr/[.sup.86]Sr was for a fish above the dam (fish 1, mean = 0.70612), clearly separating it from the possible fluvial fish below the dam (fish 3 mean = 0.70366, fish 4 mean = 0.70399). These low core values for fish 3 and 4, collected in Rock Creek below the dam, provide evidence that these 2 fish originated in the Willamette River or were spawned by mothers who completed oogenesis in waters with [.sup.87]Sr/[.sup.86]Sr values similar to the Willamette River. Based on water [.sup.87]Sr/[.sup.86]Sr data, residents that spawned in the Marys River are expected to have different core [.sup.87]Sr/[.sup.86]Sr than fluvial fish.

Dot's otolith chemistry vary across life-history transects?

In addition to the difference between edge and core values of elements, other variations are notable across otoliths. Scans of otoliths showed several peaks in Ba/Ca ratios, and sometimes Sr/Ca ratios between the core and edges in juveniles and adults (Figs. 4 to 8). These Ba/Ca and Sr/Ca peaks in the scans of adult otoliths often appeared to correspond with the clear annular growth rings (Figs. 6 to 8). In addition to differences owing to movement into waters with distinct elemental composition, otolith elemental incorporation can be affected by seasons and by changes in temperature and habitats (Kalish 1989; Radtke and Shafter 1992; Elsdon and Gillanders 2002; Miller 2011). These factors may have contributed to the patterns we observed.

Are growth rates of juveniles and adults during their 1st year similar?

Juveniles (90-135 mm in length) were assumed to be age 1 or 1+ (Moring and Yonker 1979). All adults appeared to be age 2+ or 3+. The mean and range of the otolith width at the 1st annulus was smaller in Marys River juveniles (mean = 839 [micro]m; range = 633 to 945 [micro]m) than in adults (mean = 923 [micro]m; range = 740 to 1150 [micro]m), although the difference was not significant (t = -1.21; P = 0.257). However, otolith width at the 2nd annulus was significantly smaller in the Marys River juveniles (mean = 1177 [micro]m [+ or -] 69.8 SE) than in the fluvial Willamette River adults (mean = 1490 [micro]m [+ or -] 78.9 SE) (t = -2.98; P = 0.016). This suggests potential interannual or habitat differences in growth or life-history variation, with adults having a more nutritious prey base or a different thermal regime. Sloat and Reeves (2014) found that thermal conditions influence somatic growth rates and the prevalence of resident or migratory salmonids.

DISCUSSION

Coastal Cutthroat Trout are widely distributed in tributaries of the Willamette River (Dimick and Merryfield 1945). Moring and Yonker (1979) stated that age 0 and 1 fish were rarely caught in large rivers like the Willamette, whereas these age groups account for the majority of fish in small tributaries. Whether these small fish represent recent downstream migrants from tributaries or spawning in the Willamette River is not known, but they concluded that large rivers rely on fish from tributaries for recruitment. Given the observed variation and overlap in elemental composition (Sr/Ca and Ba/Ca) of the Willamette and Marys rivers, accurately reconstructing movement patterns or life history based solely on otolith Sr/Ca and Ba/Ca is unlikely. However, there was no overlap in water [.sup.87]Sr/[.sup.86]Sr and there were clear differences in otolith edge [.sup.87]Sr/[.sup.86]Sr of fish in the Willamette and Marys rivers. We determined that otolith [.sup.87]Sr/[.sup.86]Sr ratios in the core region of adults collected in the Willamette River show that 3 of the 5 individuals with [.sup.87]Sr/[.sup.86]Sr data likely reared in the Marys River or another tributary with relatively high [.sup.87]Sr/[.sup.86]Sr. Thus, otolith [.sup.87]Sr/[.sup.86]Sr ratios hold promise for reconstructing movement history and identifying fluvial versus resident individuals within these river systems.

Although resident Cutthroat Trout populations were considered secure in most coastal streams in Oregon (Hooten 1997), they were thought to be declining in western Oregon streams where habitat has been lost and pool complexity has decreased (Reeves and others 1997). A lack of information about the status of Cutthroat Trout west of the crest of the Cascade Range, especially the fluvial types, made them a stock of concern by Wevers and others (1992). Fluvial fish were once abundant in the Marys River (Nicholas 1979) and declines are probably a result of loss of habitat complexity and pools (Reeves and others 1997). More recently, Good-son (2008) concluded that the Species Management Unit of Willamette River Coastal Cutthroat was not at risk.

Based on the otolith core values of [.sup.87]Sr/[.sup.86]Sr of the 6 juveniles sampled in the Marys River, 3 appeared to be offspring of mothers who may have resided in the Willamette River, or another tributary with similarly low [.sup.87]Sr/[.sup.86]Sr values. Thus, a preliminary estimate is that 50% of the juvenile fish from the Marys River appear to have originated from outside or from mothers that came from outside the Marys River.

Although Coastal Cutthroat Trout have phenotypically different life histories within a watershed, these life-history types are thought to be flexible, without a genetic basis for anadromous versus resident fish (Johnson and others 2010). This means that resident forms may become fluvial and vice versa. A better understanding of the relative recruitment of both resident and fluvial Cutthroat Trout to the population of the Marys River and other tributaries is needed to prioritize restoration, monitoring, and management of the different spawning and rearing habitats of these life-history types. Given the observed water and otolith edge [.sup.87]Sr/[.sup.86]Sr values, the generation of a more comprehensive watershed map of [.sup.87]Sr/[.sup.86]Sr values throughout the mainstem Willamette River (Bourret 2013) and its tributaries would be a valuable tool for reconstructing Cutthroat Trout life histories. A strontium isoscape that covers the range of Cutthroat Trout could be combined with additional fish sampling to further elucidate the relative proportions of resident and fluvial fish across the basin.

ACKNOWLEDGEMENTS

We are indebted to Karen Hans (Oregon Department of Fish and Wildlife) and Steve Trask (Bio-Surveys LLC) for their help in collecting juvenile fish from the Marys River, Randy Wildman for collecting adults in the Willamette River, Chris Romsos (Oregon State University) and Kathleen Westly (Marys River Watershed Council) for editing and formatting the manuscript, and reviewers for helpful comments.

LITERATURE CITED

ARAGUAS-ARAGUAS K, FROEHLICH K, ROZANSKI K. 2000. Deuterium and oxygen-18 isotope composition of precipitation and atmospheric moisture. Hydrological Processes 14:1341-1355.

BOURRET SL. 2013. Salmon life history in an altered landscape: Reconstructing juvenile migration using chemical and structural analysis [thesis]. Moscow, ID: University of Idaho. 97 p.

CAMPANA SE. 1999. Chemistry and composition of fish otoliths: Pathways, mechanisms, and applications. Marine Ecology Progress Series 188:263-297.

DIMICK RF, MERRYEIELD F. 1945. The fishes of the Willamette River system in relation to pollution. Bulletin Series No. 20, June 1945. Corvallis, OR: Engineering Experimental Station. Oregon State University.

ECOSYSTEMS NORTHWEST. 1989. Marys River preliminary watershed assessment. 145 p.

ELSDON TS, GILLANDERS BM. 2002. Interactive effects of temperature and salinity on otolith chemistry: Challenges for determining environmental histories of fish. Canadian Journal of Fisheries and Aquatic Sciences 59:1796-1808.

GOODSON K. 2008. The status of four species management units of Coastal Cutthroat Trout in Oregon. In: Connelly PJ, Williams TH, Greswell RE, editors. The Coastal Cutthroat Trout Symposium. Status, management, biology, and conservation. Portland, OR: Oregon Chapter, American Fisheries Society. p 5-10.

HOOTEN B. 1997. The status of Coastal Cutthroat Trout in Oregon. In: Hall JD, Bisson PA, Greswell RE, editors. Sea-run Cutthroat Trout: Biology, management, and future conservation. Corvallis, OR: Oregon Chapter, American Fisheries Society. p 57-67.

JOHNSON JR, BAUMSTEIGER J, ZYDLEWSKI J, HUDSON JM, ARDEN W. 2010. Evidence of panmixia between sympatric life history forms of coastal Cutthroat Trout in two lower Columbia River tributaries. North American Journal of Fisheries Managment 30:691-701.

KALISH JM. 1989. Otolith microchemistry: Validation of the effects of physiology, age and environment on otolith composition. Journal of Experimental Marine Biology and Ecology 132:151-176.

KENNEDY BP, BLUM JD, Four L, NISLOW KH. 2000. Using natural strontium isotopic signatures as fish markers: Methodology and application. Canadian Journal of Fisheries and Aquatic Sciences 57:2280-2292.

KENT A, UNGERER A. 2006. Analysis of light lithophile elements (Li, Be, B) by laser ablation ICP-MS: Comparison between magnetic sector and quadrupole ICP-MS. American Mineralogist 91:1401-1411.

KLAUE A, BLUM JD, FOLT CL, NISLOW KH, KENNEDY BP. 2002. Reconstructing the lives of fish using Sr isotopes in otoliths. Canadian Journal of Fisheries and Aquatic Sciences 59:925-926.

MILLER JA. 2009. The effects of temperature and water concentration on the otolith incorporation of barium and manganese in Black Rockfish Sebastes melanops. Journal of Fish Biology 75:39-60.

MILLER JA. 2011. Effects of water temperature and barium concentrations on otolith composition along a salinity gradient: Implications for migratory reconstructions. Journal of Experimental Marine Biology and Ecology 405:42-52.

MILLER JA, KENT AJR. 2009. The determination of maternal run time in Chinook Salmon (Oncorhynchus tshawytscha) based on Sr/Ca and [.sup.87]Sr/[.sup.86]Sr within otolith cores. Fisheries Research 95:373-378.

MORING JR, YONKER R. 1979. Oregon Rainbow and Cutthroat Trout evaluation. Fish Division Final Report, F-94-R. Salem, OR: Oregon Department of Fish and Wildlife,

MORING J, YONKER R, HOOTON R. Undated. Movement patterns of potadromous Cutthroat Trout of the Willamette Valley, Oregon. Corvallis, OR: Oregon Department of Fish and Wildlife, Research Division.

MUHLFELD CC, THORROLD SR, MCMAHON TE, MARTOZ B. 2012. Estimating Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi) movements in a river network using strontium isoscapes. Canadian Journal of Fisheries and Aquatic Sciences 69:906-915.

NICHOLAS JW. 1978. A review of literature and unpublished information on Cutthroat Trout (Salmo clarki clarki) of the Willamette watershed. Salem, OR: Oregon Department of Fisheries and Wildlife, Information Report Series, Fisheries N 78-1.

PEARCY WG, BRODEUR RD, MCKINNELL SM, LOSEE J. In press. Anadromous coastal Cutthroat Trout Oncorhychus clarkii clarkii). American Fisheries Society.

RADTKE RJ, SHAFTER DJ. 1992. Environmental sensitivity of fish otolith microchemistry. Australian Journal of Marine and Freshwater Research 43:935-951.

REEVES G, HALL J, GREGORY S. 1997. The impact of land-management activities on Coastal Cutthroat Trout and their freshwater habitats. In: Hall JD, Bisson PA, Greswell RE, editors. Sea-run Cutthroat Trout: Biology, management, and future conservation. Corvallis, OR: Oregon Chapter, American Fisheries Society.

SLOAT MR, REEVES GR. 2014. Individual condition, standard metabolic rate, and rearing temperature influence steelhead and Rainbow Trout (Oncorhynchus mykiss) life histories. Canadian Journal of Fisheries and Aquatic Sciences 71:491-501.

TROTTER PC. 1989. Coastal Cutthroat Trout: A life history compendium. Transactions of the American Fisheries Society 118:563-473.

VOLK EC, BLAKLEY A, SCHRODER SL, KUEHNER SM. 2000. Otolith chemistry reflects migratory characteristics of Pacific salmonids: Using otolith core chemistry to distinguish maternal associations with sea and freshwaters. Fisheries Research 46:251-266.

WEBER PK, BACON CR, HUTCHEON ID, INGRAM BL, WOODEN JL. 2005. Ion microprobe measurement of strontium isotopes in calcium carbonate with application to salmon otoliths. Geochimica et Cosmochimica Acta 69:1225-1239.

WELLS BK, RIEMAN BE, CLAYTON JL, HORAN DL. 2003. Relationships between water, otolith, and scale chemistries of Westslope Cutthroat Trout form the Coeur d'Alene River, Idaho: The potential application of hard-part chemistry to describe movements in freshwater. Transactions of the Amererican Fisheries Society 132:409-424.

WEVERS M, NEMETH D, HAXTON J, MAMOYAC S. 1992. Coast Range subbasin fish management plan. Corvallis, OR: Oregon Department of Fish and Wildlife, Northwest Region.

WOODHEAD J, SWEARER S, HERGTA J, MAASA R. 2005. In situ Sr-isotope analysis of carbonates by LA-MC-ICP-MS: Interference corrections, high spatial resolution and an example from otolith studies. Journal of Analytical Atomic Spectrometry 20:22-27.

ZIMMERMAN CE, REEVES GH. 2002. Identification of steelhead and resident Rainbow Trout progeny in the Deschutes River, Oregon, revealed with otolith microchemistry. Transactions of the American Fisheries Society 131:986-993.

ZYDLEWSKI GB, ZYDKEWSKI J, JOHNSON J. 2009. Patterns of migration and residency in Coastal Cutthroat Trout Oncorhynchus clarkii clarkii from two tributaries of the lower Columbia River. Journal of Fish Biology 75:203-222.

WILLIAM G PEARCY AND JESSICA A MILLER

College of Earth, Ocean, and Atmospheric Sciences and Department of Fisheries and Wildlife, Oregon State University, Corvallis, OR 97337 USA; pearcyw@oregonstate.edu

Submitted 9 August 2017, accepted 22 March 2018. Corresponding Editor: Tara Chestnut.
TABLE 1. Water chemistry data collected from the Marys River and
tributaries in March and May 2012, and the Willamette River in March
and August 2012. "NA" = data not available.

Site                              Collection month  Ba/Ca ([micro]M/M)

Tumtum River at Ellmaker          March             2500
Tumtum River at Blodgett          March             1190
Rock Creek                        March              300
Rock Creek                        August             190
Rock Creek 2                      August             120
Rock Creek 2                      August              50
Blair Creek at Hwy 20             August             180
Wood Creek                        March              210
Marys River at Blodgett           March             1750
Willamette River Park             March              220
Willamette River at Irish Bend    March              160
Willamette River near Harrisburg  August            NA

Site                              Sr/Ca (mM/M)  [.sup.87]Sr/[.sup.86]Sr

Tumtum River at Ellmaker          7.69          0.70793
Tumtum River at Blodgett          4.35          0.70746
Rock Creek                        1.18          0.70478
Rock Creek                        1.82          NA
Rock Creek 2                      1.23          NA
Rock Creek 2                      1.29          NA
Blair Creek at Hwy 20             1.96          0.70509
Wood Creek                        1.34          0.70551
Marys River at Blodgett           6.11          0.70756
Willamette River Park             3.16          0.70412
Willamette River at Irish Bend    3.15          0.70388
Willamette River near Harrisburg  3.1           NA

Site                              [delta][.sup.18]O

Tumtum River at Ellmaker           -7.8
Tumtum River at Blodgett          NA
Rock Creek                         -8.9
Rock Creek                        NA
Rock Creek 2                      NA
Rock Creek 2                      NA
Blair Creek at Hwy 20             NA
Wood Creek                         -9.1
Marys River at Blodgett            -8.2
Willamette River Park             -11.1
Willamette River at Irish Bend    -11.4
Willamette River near Harrisburg  NA

TABLE 2. Fish identification number (ID), size, collection site, and
mean ([+ or -] standard deviation) for [.sup.87]Sr/[.sup.86]Sr in the
otolith core and edge for juveniles collected in March and May, and for
the adults caught in August. For juveniles, bold text denotes
individuals whose core chemistry indicates a probable Willamette River
maternal origin. For adults, bold text denotes individuals that
appeared to rear outside of the Willamette River in waters similar to
portions of the Marys River and its tributaries. The mean Willamette
River otolith edge [.sup.87]Sr/[.sup.86]Sr = 0.70377 [+ or -] 0.00019
SD (957r CI = 0.70361-0.70393). The mean otolith edge
[.sup.87]Sr/[.sup.86]Sr for juveniles collected in the Marys River
basin = 0.70512 [+ or -] 0.00066 SD (95% CI = 0.70458-0.70565).


Fish ID    Fork length (mm)  Collection site

juveniles
 1         124               Rock Ck. 1
 2          90               Rock Ck. 1
 3         115               Rock Ck. 2
 4         101               Rock Ck. 2
 5         190               Rock Ck. 1
 6         135               Wood Ck. 1
 7         102               Wood Ck. 2
10         110               Tumtum at Blodgett
11          85               Blair Ck.
14          98               Wood Ck. 2
Adults
 1         315               Willamette R.
 2         220               Willamette R.
 3         223               Willamette R.
 4         268               Willamette R.
 5         303               Willamette R.
 6         294               Willamette R.

           [.sup.87]Sr/[.sup.86]Sr
Fish ID    Otolith core               Otolith edge

juveniles
 1         0.70612 ([+ or -]0.00046)  0.70590 ([+ or -]0.00028)
 2         NA                         NA
 3         0.70366 ([+ or -]0.00036)  0.70470 ([+ or -]0.00028)
 4         0.70399 ([+ or -]0.00052)  0.70428 ([+ or -]0.00133)
 5         0.70474 ([+ or -]0.00025)  0.70556 ([+ or -]0.00051)
 6         0.70567 ([+ or -]0.00032)  0.70563 ([+ or -]0.00063)
 7         0.70408 ([+ or -]0.00046)  0.70462 ([+ or -]0.00041)
10         NA                         NA
11         NA                         NA
14         NA                         NA
Adults
 1         0.70574 ([+ or -]0.00038)  0.70393 ([+ or -]0.00012)
 2         0.70387 ([+ or -]0.00036)  0.70396 ([+ or -]0.00015)
 3         0.70461 ([+ or -]0.00097)  0.70352 ([+ or -]0.00013)
 4         NA                         NA
 5         0.70732 ([+ or -]0.00029)  0.70379 ([+ or -]0.00034)
 6         0.70392 ([+ or -]0.00025)  0.70365 ([+ or -]0.00019)

TABLE 3. Fish identification number (ID), size, collection site, and
mean and standard error for otolith Ba/Ca ([micro]M/M) and Sr/Ca (mM/M)
at the core and edge (juveniles in March and May) and for the core and
edge during rearing for the adults caught in August.


                                     Otolith core
Fish ID    (mm)  Collection site     Ba/Ca ([micro]M/M)  Sr/Ca (mM/M)

Juveniles
 1         124   Rock Ck 1            4.8 (0.39)         0.60 (0.04)
 2          90   Rock Ck 1            7.0 (0.52)         0.39 (0.02)
 3         115   Rock Ck 2            3.0 (0.44)         0.45 (0.05)
 4         101   Rock Ck 2            3.8 (0.37)         0.74 (0.06)
 5         140   Rock Ck 1            2.7 (0.36)         0.44 (0.04)
 6         135   Wood Ck 1            4.3 (0.40)         0.38 (0.03)
 7         102   Wood Ck 2            8.8 (0.95)         0.73 (0.06)
10         110   Tumtum at Blodgett  43.8 (5.8)          2.02 (0.24)
11          85   Blair Ck             6.0 (0.52)         0.45 (0.03)
14          98   Wood Ck 2            3.5 (0.52)         0.48 (0.04)
Adults
 1         315   Willamette R.        9.0 (0.62)         0.33 (0.02)
 2         220   Willamette R.        4.4 (0.33)         0.54 (0.04)
 3         223   Willamette R.        0.9 (0.11)         0.20 (0.04)
 4         268   Willamette R.        1.2 (0.11)         0.56 (0.04)
 5         303   Willamette R.        9.0 (1.35)         0.76 (0.07)
 6         294   Willamette R.        8.0 (0.90)         0.70 (0.06)

           Otolith edge
Fish ID    Ba/Ca ([micro]M/M)  Sr/Ca (mM/M)

Juveniles
 1          6.0 (0.56)         0.59 (0.08)
 2          5.7 (1.09)         0.36 (0.02)
 3          1.7(0.21)          0.54 (0.05)
 4          1.0(0.34)          0.42 (0.06)
 5          8.3 (1.59)         0.56 (0.47)
 6         13.0 (1.36)         0.55 (0.05)
 7          5.6 (0.66)         0.39 (0.02)
10         28.2 (2.71)         2.07 (0.18)
11          2.6 (0.23)         0.42 (0.03)
14          2.0 (0.52)         0.41 (0.02)
Adults
 1          1.9 (0.17)         0.69 (0.05)
 2          5.5 (0.70)         0.67 (0.07)
 3          6.8 (2.38)         0.72 (0.06)
 4          2.1 (0.32)         0.70 (0.05)
 5          2.3 (0.25)         0.66 (0.05)
 6          2.0 (0.19)         0.65 (0.05)
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Author:Pearcy, William G.; Miller, Jessica A.
Publication:Northwestern Naturalist: A Journal of Vertebrate Biology
Article Type:Report
Geographic Code:1U9OR
Date:Sep 22, 2018
Words:5332
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