Printer Friendly


Byline: J. Broucek


The goal of this review was to analyze published data on practices that mitigate enteric methane (CH 4) emissions from ruminants. The current approaches in relation to associated advantages and disadvantages and future options to reduce enteric CH4 emission are presented. Strategies for reducing ruminant CH4 output are considered in relationship to rumen ecology, biochemistry, and animal performance. The study is divided into nine sections (Defaunation and inhibition of archaea, Bacteriocins, Methane inhibitors and analogues, Probiotics, Saponins, Tanins, Ionophores, Organic acids, and Lipids). Defaunation or elimination of protozoa to reduce CH4 emission depending upon diet. Mitigation of CH4 emission by rumen microbes can be to apply the various chemicals. Some approaches such as immunization and chemical inhibitors directly target against their own rumen methanogens. Organic acids feeding promote propionate production.

A reduction in CH4 production has also been observed with live yeast cells, lactate-utilizing bacteria, and by the selection of plant species that produce secondary metabolites, such as condensed tannins and saponins. Supplements rich in polyunsaturated fat acids such as linoleic acid, and linolenic acid also have a negative effect on CH4. Based on available results, it appears that dietary supplementation with fat or essential oils are the most promising dietary strategy.

Keywords: Environment, Methane, Ruminant, Additive


Methane emissions in animal husbandry originate from fermentative digestion in animals, natural anaerobic ecosystems, storage of manures, and field application. Within livestock, ruminants (cattle, sheep, and goats) are the primary source of emissions. The actual rate of methane (CH4) emission is highly dependent on the management strategies implemented on a farm (Kirchgessner et al., 1991; Dammgen et al., 2012; Broucek, 2015; Van Middelaar et al., 2013).

Integrated research investigating animal, plant, microbe and nutrient level strategies might offer a long term solution of CH4 production abatement. At the animal level, genetic selection is the area of research with the best chance of finding a solution. Methane emissions can be reduced by the selection of plant species that produce secondary metabolites to reduce methanogenesis, such as saponins, flavonoids and tannins.

Methods of inhibiting methanogens include use of antibiotics, feed additives (fats and oils), nitrate salts, or dicarboxylic acids. The defaunation, vaccination against methanogens, halogenated and chlorinated analogues, bromo-chloromethane, and cyclodextrin are also importat treatments. Methods of enhancing alternative hydrogen (H2) using microbial species include inoculating with acetogenic species, feeding highly digestible feed components favouring propionate fermentations, and modifying rumen conditions (Cottle et al., 2011).

The sustainability of methane suppressing strategies is an important issue. There is an urgent need for support model that is capable of evaluating the effectiveness of both existing and new technologies for reducing methane emission (Sejian and Naqvi, 2012; Mihina et al., 2012). Despite intensive research in this area, our understanding is far from complete. There have been many reports showing different responses to feed additives such as ionophores, yeast products, fumaric acid, etc., and there is considerable difficulty in applying in vitro data to in vivo experiments due to the complexity of the ruminal system (Moss, 1994; McGinn et al., 2004; Shibata and Terada, 2010). However, such research will be expensive and long term.

Defaunation and inhibition of archaea: Defaunation and vaccines are mitigation techniques which target the methanogen population of the rumen directly or indirectly, resulting in varying degrees of efficacy (Hook et al., 2010). Strategies are following: a decreasing of the number of the methanogenic archaea (archaebacteria); a reduction of H2 production; a stimulation of H2 utilisation beneficial for the animal.

In ruminants, the major portion of the methanogenesis occurs in the the rumen, where feeds including fibrous plant structures are fermented primarily to short-chain volatile fatty acids, carbon dioxide (CO2), hydrogen, and methane by bacteria, protozoa, fungi and methanogens. The methanogens belong to a separate domain archaea in the kingdom of Euryarchaeota and are found in a wide range of other anaerobic environments (Liu and Whitman, 2008; Hook et al., 2010).

Methanogens are very diverse in terms of phylogeny and ecology. They play an important role in the anaerobic food chain, driving anaerobic fermentation through removal of excess H2 and formate. Most rumen methanogens derive energy for their growth through a series of biochemical reduction of CO2 with H2, and some methanogens use acetate and methyl group-containing compounds to produce methane (methanogenesis): 4 H2 + CO2 = CH4 + 2 H2O

Among 28 genera and 113 species of methanogens known to be present in nature, only seven species have commonly been cultured from the rumen (Janssen and Kirs, 2008; Hook et al., 2010). These are Methanobacterium formicicum, Methanobacterium bryantii, Methanobrevibacter ruminantium, Methanobrevibacter millerae, Methanobrevibacter olleyae, Methanomicrobium mobile, and Methanoculleus olentangyi. Analysis of molecular-based studies reveals that the members of family Methanobacteriaceae (which includes Methanobrevibacter spp., Methanobacterium spp., and Methanosphaera spp.) are the dominant members (30 % to 99 % of archaea) of the rumen archaea. Members of the order Methanomicrobiales (which includes Methanomicrobium spp.) are less abundant (0 % to 54 %), and members of the order Methanosarcinales (which includes Methanimicrococcus) are rare (2 % to 3 %).

Usually, CH4 is produced by two types of methanogens, the slow-growing methanogens that produces CH4 from acetate (e.g., Methanosarcina) and fast growing methanogens that reduce CO2 with H2.

Most of the ciliate protozoa living in the rumen, they are not present at birth but normally start establishing in the gastrointestinal tract 3 weeks after birth (Iqbal et al., 2008). Ciliate protozoa are known to affect methanogens in a generic and age manner. There were found to have intracellular bacteria with methanogens (Finlay et al., 1994). The elimination of ciliate protozoa from the rumen has been proposed as a means of increasing the productivity of ruminants (Finlay et al., 1994). The importance of methanogenic bacteria associated with ciliate protozoa was estimated either by removing protozoa from whole rumen fluid or by isolating the protozoa. Methanogenic bacteria associated with rumen ciliates were responsible for between 9 and 25 % of methanogenesis in rumen fluid of sheep (Newbold et al., 1995a).

Defaunation techniques used in research have been reviewed by Hegarty (1999) and Iqbal et al. (2008). Defaunation approaches include dietary manipulation (virginiamycin, milk fat); synthetic chemicals including copper sulfate, calcium peroxide, dioctylsodium sulfosuccinate and detergents; and natural compounds like vitamin A, non-protein amino acids and ecdysones, the steroidal hormones which cause skin shedding in insects. Many of the most feed supplements and additives have the ability to reduce protozoal numbers. Some lipids, saponins, tannins and ionophores are toxic to protozoa.

Defaunation effects on mitigated methane production due to (1) reduced fiber digestion, (2) reduced methanogen population associated with protozoa (Machmuller et al., 2003), (3) reduced hydrogen transfer (Finlay et al., 1994), and (4) increased partial pressure of oxygen on the rumen (Eckard et al., 2010).

The removal of protozoa from the rumen has been shown to reduce CH4 production by up to 50 % (Hegarty, 1999; Martin et al., 2010). Shibata and Terada (2010) described 20 - 40 % reduction in CH4 production by defaunation. Defaunation reduces methane production by 20 to 50 % depending upon diet (Mathison et al., 1998). Kreuzer et al. (1986; cited by Iqbal et al., 2008) reported that defaunation of cattle fed a high concentrate diet decreased methane production by 50 %. CH4 production was the lowest in defaunated calves and the calves with a mixed protozoan population showed the highest CH4 production (Shibata and Terada, 2010). Kurihara et al. (1997, cited by Shibata and Terada, 2010) wrote that ruminal numbers of protozoa and of cellulolytic bacteria tend to decrease in dry cows fed zinc sulfate and that CH4 production showed a 60 % reduction. Hegarty (1999) concluded from reviewed data that the defaunation method results in 40 % of methanogenesis in rumen fluid.

Machmuller et al. (2003) found that effects of myristic acid supplementation inhibited significantly rumen archaea without significantly altering proportions of individual methanogen orders in sheep. This decreased daily CH4 emission of the animals by 40 % on average. Ciliate protozoa concentration was decreased.

The decrease in CH4 production of 26 % per kg of dry matter intake (DMI) in protozoa-free lambs was related to a decrease in the proportion of methanogens in the total bacterial population of the whole ruminal content (McAllister and Newbold, 2008; Martin et al., 2010). The study of Morgavi et al. (2008) show that the methane emissions decrease of induced by defaunation was stable for to 2 years in sheep. In the absence of protozoa, CH4 decreased by 20 % in both short-and long-term defaunated animals. Dohme et al. (1999) showed that CH4 production decreased by 61 % in defaunated rumen fluid.

Another method involves defaunation of the rumen to remove syntrophic hydrogen-producing protozoa (Hook et al., 2010; Finn et al., 2015). Methanogenesis is an important liquidating activity for H2, which is the key element to consider for reducing CH4 production (Joblin, 1999). The H2 production and methanogenesis could be altered by increasing the quantity of microbial cells leaving the rumen per unit of carbohydrate consumed. The microbial ecology of the rumen ecosystem is exceedingly complex and the ability of this system to efficiently convert complex carbohydrates to fermentable sugars is in part due to the effective disposal of H2 through reduction of CO2 to methane by methanogens (McAllister and Newbold, 2008). Methanogenic microorganisms remove H2 produced during fermentation of organic matter in the rumen and hind gut (Cottle et al., 2011).

In this respect, acetogenic bacteria normally present in the rumen are of interest because they have the potential to provide an alternative sink for H2, an essential intermediate in the formation of CH4. Studies on ruminants fed diets containing concentrates or conserved forages indicate that the rumen contains a diversity of acetogens. In the rumen ecosystem, the ubiquitous protozoa are large producers of this metabolic end product. In addition, a physical association between protozoal cells and methanogens exist in the rumen ecosystem that favours H2 transfer. The methanogens found both attached and inside ciliate protozoal cells have been estimated to contribute between 9 % and 37 % of the rumen methanogenesis (Finlay et al., 1994; Newbold et al., 1995a).

Some problems are associated with defaunation. Digestion will be negatively affected if animals are defaunated completely (Machmuller et al., 2003). In addition, the restoration of CH4 emissions to pre-treatment levels seen for some products has been associated to an adaptation and recovery of protozoal numbers (Martin et al., 2010). Other reports indicate that the reduction of methane production by defaunation is only temporary (Iqbal et al., 2008). However, in the study of Morgavi et al. (2008), the lower CH4 emission in defaunated animals was maintained for more than 2 years indicating that the changes induced are stable.

Elimination of the rumen protozoa to mitigate methanogenesis is promising, but this option should be carefully evaluated in terms of livestock welfare and performances. In addition, on-farm defaunation techniques are not available up to now. Several feed additives such as ionophores, organic acids and plant extracts have also been assayed (Martin et al., 2010; Guerci et al., 2013). The absence of protozoa from the rumen can have diverse effects on animals that can be either negative or positive depending on the diet and the type of production targeted (Martin et al., 2010).

However, this technique is difficult to apply in the field. Further research is required to identify the feeding conditions that play a role in the effect of defaunation on CH4 production and to establish practical methods of applying the technique of defaunation. Up to now, however, practical defaunation techniques are not available.

Several biotechnological strategies of immunisation and biological control are currently being explored. The targeted manipulation of the rumen ecosystem provides the best hope for mitigating enteric CH4 emissions and the biggest challenge. However, vaccines prepared with a different set of methanogen species or tested in other geographical regions did not elicit a positive response. The highly diverse methanogenic community in animals reared under different conditions maybe guilty (Wright et al., 2007).

According to Cook et al. (2008) experiment, passive immunisation was assayed using antibodies, which were produced in laying hens, against three common methanogens present in the digestive tract of animals. They reported that opinion an ideal treatment for inhibiting methanogenesis would reduce CH4 production without unfavourably altering ruminal fermentation and VFA profiles, or inhibiting synthesis of microbial protein. One possible future pathway to reduce CH4 output is to immunize animals against their own methanogens and protozoa. Baker et al. (1997; cited by Iqbal et al., 2008) have claimed to invoke an immune response to rumen protozoa by administering an immunogenic preparation. This can affect the activity of rumen methanogens as they have a relationship with rumen protozoa.

The development of an antimethanogenic vaccine is also in progress. It is anticipated that vaccination could reduce CH4 output up very significant. However, the results are still generally inconclusive.

Wright et al. (2004) found those 8 to 10 weeks after primary immunization, no significant differences in CH4 production could be measured between the three treatments, although there were trends of an 8 % and 6 % reduction when compared to the control sheep. When CH4 data were expressed per kg of DMI, sheep immunized with the 3-methanogen mix and the 7-methanogen mix were 4 % and 5 % less, respectively, than the control sheep. Furthermore, authors decided to administer a secondary immunization. As a result, sheep in the 3-methanogen mix group produced 12.8 % less methane than sheep in the control group. Even when the data were corrected for DMI, there was a significant 7.7 % reduction in methane production per kg of DMI between the control and the treatment sheep. Williams et al. (2009) described a vaccination regimen which induced a substantial serum antibody response against methanogens in sheep but failed to significantly affect the CH4 emission by these sheep and the density of methanogens.

Vaccines against rumen archaea may offer mitigation opportunities in the future. However, the CH4 reduction is to be small and adaptation by ruminal microbes and persistence of the effect is unknown. Much more work is needed to make this technique effective, as there are multiple strains of Archaea in the rumen. If this proves successful, the vaccination would be a valuable tool for CH4 reductions as it could be applied to a whole ruminant population (Hristov et al., 2013a).

This approach has the benefit of using the animal's immune system to produce antibodies against specific methanogens, instead of chemicals, drugs, or antibiotics that may be potentially harmful to the animal or the environment. Moreover, this reduction in methane was achieved despite targeting probably less than 20 % of the methanogens in the rumen. Whether or not greater efficacy can be achieved using more targeted vaccine formulations, or different feedstuffs, requires elucidation. Up to now, immunisation has not delivered a clear, positive answer in reducing CH4 emissions by ruminants, highlighting the difficulties of this approach (Martin et al., 2010).

The most promising avenues for future research for reducing methanogenesis are the development of new products for reducing protozoal numbers in the rumen and the use of bacterocins or other compounds which specifically target methanogenic bacteria (Mathison et al., 1998).

Bacteriocins: Bacteriocins are proteinaceous toxins produced by bacteria to inhibit the growth of bacterial strain (Renuka et al., 2013; Guerci et al., 2013) which are typically considered to be of narrow spectrum antibiotics. Some bacteriocins (nisin and bovicin) are known to reduce CH4 production. McAllister and Newbold (2008) reported that bacteriocins could prove effective in directly inhibiting methanogens and redirecting H2 to other reductive bacteria, such as propionate producers or acetogens.

Nisin, obtained from Lactobaccilus lactis ssp. lactis, has been shown to decrease methane production in vitro (Martin et al., 2010). Although the mechanism is still unclear, nisin reduce rumen methanogenesis by 36 % (Callaway et al., 1997). Santoso et al. (2004) found decreased methane emission from sheep fed on basal diet supplemented with nisin. However, nisin, in addition to Yucca schidigera, had only minor effects on rumen fermentation (Santoso et al., 2006).

Bovicin HC5, another bacteriocin produced by Streptococcus bovis from the rumen, has been reported to suppress methane production in vitro by 50 % (Lee et al., 2002). The use of bacteriocins may be prospective for inhibiting methanogen populations in the rumen. According to Lima et al. (2009) found in vitro studies that HC5 may be useful in limiting CH4 production in the rumen. Bacteriocins may provide an alternative strategy for decreasing ruminal CH4 production.

Methane inhibitors and analogues: Methane inhibitors are chemical compounds with inhibitory effects on rumen archaea. The formation of methane by the rumen microbiota is also reduced by the methane analogues in the presence or absence of added substrate. These inhibitors affect one or more of the reactions by which methane is formed from hydrogen and carbon dioxide (Bauchop, 1967; Hristov et al., 2015). Control of CH4 emission by microbes has mainly been focused on application of chemicals that inhibit the growth and activity of methanogens in the rumen (Song et al., 2011). Some inhibitors have strong negative effect on methanogenesis. Lanigan (1972) showed five halogenated methane analogues (bromoform, chloroform, iodoform, carbon tetrabromide, and carbon tetrachloride) to inhibit CH4 creating in the sheep's rumen as well as in rumen fluid in vitro.

Other authors (Van Nevel and Demeyer, 1996; McCrabb et al., 1997; Song et al., 2011; Patra, 2014) reported also hydroxylated and chlorinated analogues, cyclodextrin, bromochloromethane, bromoethanesulphonate, chloral hydrate, and bromine analogue of coenzyme M. Chloroform, as chlorinated methane analogue, depress methanogenesis through inhibition of methyl-CoM reductase but is obviously not suitable for field application. There were found significant reduction in CH4 production by chloral hydrate, but prolonged feeding led to liver damage (Van Nevel and Demeyer, 1996). Low concentrations of chloroform, carbon tetrachloride, and methylene chloride inhibited the formation of CH4 by the rumen microbiota in the presence or absence of added substrate (Thiel, 1969).

McCrabb et al. (1997) conducted trials to determine the effects of dietary supplementation with a complex of bromochloromethane and [alpha]-cyclodextrin. Methane production of treated steers was significantly lower than that of control steers over 28 days.

Dumitru et al. (2003) found that 4-(ss-D-ribofuranosyl) aminobenzene-5-phosphate (RFA-P) synthase catalyzes the biosynthesis of methanopterin which stop the growth of methane-producing microbes. RFA-P is a key cofactor required for methane formation and for one-carbon transformations in methanogens, but its effects lasted only 3 days in sheep. In vitro experimental results indicated that none of these inhibitors affect bacterial metabolism adversely. Supplementation with hydroxylated analogue of methionine tended to decrease CH4 production further (Patra, 2014).

Bromoethanesulphonate (BES), structural analogue, as the cofactor mercaptoethanesulfonic acid (a bromine analogue of coenzyme M) is also a potent inhibitor of CH4 emission (Mathison et al., 1998). BES involved in methyl group transfer during methanogenesis, is a potentially strong inhibitor of CH4 production. Lee et al. (2009) observed a reduced in vitro CH4 production by supplementing of BES to the culture solution containing timothy or mixed substrate in a dose dependent manner. BES at 5 mM concentration inhibited CH4 production by more than 95 % compared to the control. Bromochloromethane appeared to be a potent CH4 inhibitors (Mathison et al., 1998), but its effect on ruminal methanogenesis was transient (Van Nevel and Demeyer, 1996).

Methane inhibitors can reduce CH4 emissions to zero in the short term but due to microbial adaptation the effects of these compounds are quickly neutralized and feed intake is often depressed (Mathison et al., 1998). Basic pathway of CH4 production in the bovine rumen is the reduction of CO2 with molecular H2. In rumen liquid, H2 is normally converted to CH4 (Bauchop, 1967). Methods of enhancing H2 using microbial species include: inoculating with acetogenic species; feeding highly digestible feed components favouring propionate fermentations; and modifying rumen conditions (Cottle et al., 2011). Methanogenic microorganisms remove H2 produced during fermentation of organic matter in the rumen and hind gut. As a consequence, the amount of H2 produced during fermentation is reduced, resulting in decreased CH4 production (Hristov et al., 2013b; Alstrup et al., 2015).

Propionate enhancers are also one of the effective alternatives in methane control. Fumarate and malate are carbon intermediates in the propionate pathway. In this reaction, hydrogen ion (H+) is needed and therefore, reduction of the dicarboxylic acids may provide an alternative electron sink for H2 (Morgavi et al., 2010; Song et al., 2011). Any mitigation strategy that reduces methanogen populations must also include an alternative pathway for H2 removal from the rumen.

Supplementing nitrate (NO3) to ruminants has been shown to decrease methane production (Zijderveld van et al., 2011). Nitrate has a greater affinity for H2 than does CO2 and so, when nitrate is present in the rumen; nitrite and ammonia formation is favoured over methane production. Stoichiometrically, 1 kg NO3 reduces methane formation by 258.7 g CH4 (Cottle and Eckard, 2014). The enteric CH4 was 19.6 % reduced in sheep and 18.2 % in goats fed diet containing 5 % sodium nitrate with sulphur and 0.4% sodium nitrate without sulphur compared to those fed control diet (Arif et al., 2016).

Probiotics: Probiotics are microbial feed additives (the live microorganisms) that influence rumen fermentation and improve health by modulating gut microbiota (Patel et al., 2015). The most widely used probiotics are yeast, Saccharomyces cerevisiae, Lactobacillus sporogenes and some others. The principle is not yet clear, however, it is assumed that yeast cultures reduce methane production in more manners.

Probiotics increase butyrate or propionate production. The results of Lila et al. (2004) showed that a twin strain of Saccharomyces cerevisiae live cells stimulated in vitro mixed ruminal microorganism fermentation with decreased lactate, and a small decrease of CH4 and H2 with hay plus concentrate. The decrease in CH4 production may be due to the utilization of metabolic H2 by acetogenic bacteria to produce acetate. Another effect of probioticsis decrease numbers of protozoa. Therefore, the presence of yeast would be beneficial to the rumen microflora. According to Newbold et al. (1996), the protozoal population was significantly lower in yeast supplemented. Newbold et al. (1995b) and Newbold et al. (1998) suggest another possibility, that yeast decreases numbers of rumen ciliate protozoa.

Probiotics increasing microbial synthesis and amplify acetogenesis (Chaucheyras et al., 1995; Chaucheyras-Durand et al., 2008). The positive effects of a live strain of Saccharomyces cerevisiae on H2 utilization, acetate and CH4 production by two hydrogenotrophic ruminal microorganisms, an acetogenic bacterial strain and an archaea methanogen, were investigated (Doreau and Jouany, 1998). The addition of yeast cells enhanced by more than 5 times the hydrogenotrophic metabolism of the acetogenic strain and its acetate production. The use of yeasts as ruminant feed additives could help these bacteria to compete (Chaucheyras-Durand et al., 2008).

Wallace and Newbold (1993, cited by Iqbal et al., 2008) found that probiotics improved productivity by 7-8 % resulting in reduced CH4 production per unit of product in dairy cows and growing cattle. Mwenya et al. (2004) evaluated effects of adding yeast culture, lactic acid bacteria and galacto-oligosaccharides on rumen methanogenesis in sheep. They reported that sheep produced by 10 % less CH4 when 4 g yeast culture was included per day in a diet (40 % timothy hay, 30 % alfalfa hay, and 30 % concentrate).

McGinn et al. (2004) reported that reduction in CH4 by the yeast was small and not statistically significant in cattle. Study has also shown that CH4 suppression effects of probiotics are not consistent.

The use of probiotics or the stimulation of rumen microbial populations capable to decrease CH4 emissions remains a potentially interesting approach. The use of probiotics, i.e. live yeasts, remains a potentially interesting approach, but results have been either unsatisfactory, not conclusive, or have yet to be confirmed in vivo (Martin et al., 2010).

Saponins: Saponins are glucosides with foaming characteristics that have a direct effect on rumen microbes. They are occurring in many plant species. Saponins are classified based on their carbon skeletons, 11 main classes of saponins were distinguished: dammaranes, tirucallanes, lupanes, hopanes, oleananes, taraxasteranes, ursanes, cycloartanes, lanostanes, cucurbitanes, and steroids (Vincken et al., 2007). Teferedegne et al. (1999) evaluated 9 different accessions of Sesbania sesban for their toxicity to ciliate protozoa. They showed that leaves of Sesbania sesban defaunated the rumen of sheep. Saponins decrease protein degradation and favour at the same time microbial protein and biomass synthesis, two processes that result in reduced availability of H2 for CH4 production (Goel et al., 2008a; Martin et al., 2010).

Research indicates that some saponins might actually be beneficial to rumen digestion. However, saponins are bitter and reduce the palatability of livestock feeds. Some saponins reduce therefore the feed intake and growth rate of nonruminant animals (Das et al., 2012). Results of Goel et al. (2008b) indicate that amongst the three plants tested, Carduus pycnocephalus has the highest potential reduced CH4 level by 20-21.5 % on hay or concentrate-hay (68: 32) based diets. The saponin containing feeds, fenugreek (Trigonella foenum-graecum L.) seeds and Sesbania seban did not produce significant reduction in CH4 production despite decrease in the protozoal numbers by nearly 50 %. Bodas et al. (2008) tested 450 plant samples. 35 decreased methane production by more than 15 % versusthose with corresponding control cultures and six species (C. pycnocephalus, P. tremula, Prunus avium, Q. robur, R. nobile and S. caprea).

Two plants, Rheum nobile and Carduus pycnocephalus, consistently decreased methane production without adversely affecting other parameters of the rumen fermentation.

Certain saponin-rich fruits of tropical multipurpose trees could have the potential to reduce methane emission. A comparison of the effects on rumen fermentation of three saponin rich tropical fruits (Sapindus saponaria, Enterolobium cyclocarpum, and Pithecellobium saman) supplemented to forage-based diets was done by Hess et al. (2003a). Only Sapindus saponaria decreased protozoal count by 54 % and daily CH4 release by 20 % relative to control diet, but without affecting the methanogen count. Another articleby the same authors (Hess et al., 2003b) pointed that fruits of Sapindus saponaria had a methane-suppressing potential when were added to a high-fibre diet containing the tropical high-quality legume Arachis pintoi. Sapindus saponaria reduced methanogenesis by 11 % on average in grass-alone and legume-supplemented diets. According to Hess et al. (2004), supplementation with fruits of S. saponaria increased total bacteria count, and decreased total ciliate protozoa count.

The results confirmed that it could be a useful supplement in reducing CH4 emission per animal without affecting N and energy retention of sheep given tropical grass-concentrate diets alone or in combination with a legume.

Pen et al. (2006) evaluated effects of increasing concentrations of Yucca schidigera and Quillaja saponaria extracts on rumen fermentation and CH4 production of non-lactating Holstein cows fed oat and alfalfa hay. Methane production was strongly inhibited by Yucca schidigera but not by Quillaja saponaria extract. This suggests that type and origin of saponins alter rumen fermentation differently. An experiment of Holtshausen et al. (2009) was conducted to determine whether the addition of saponin-containing Yucca schidigera or Quillaja saponaria reduces CH4 production without impairing ruminal fermentation or fiber digestion. The increasing levels of both saponin sources decreased CH4 concentration in the space of dairy cows. A lower feeding rate of both saponin sources (10 of dry matter) was used in vivo. Feeding saponin did not affect milk production, rumen fermentation, or CH4 production. However, efficiency of milk production was lower for cows fed saponin compared with controls.

The Hu et al. (2005) study was conducted to investigate the effect of tea (triterpenoid) saponins on ruminal fermentation in vitro and found that tea saponins significantly inhibited the protozoa growth in ruminal fluid. It is suggested that they could modify the rumen fermentation and inhibit the release of CH4. Using higher doses of tea saponin (8 mg) on 200 mg of substrates in ruminal fluids, authors recorded a 79 % decrease in protozoan counts and 26 % decline in in vitro CH4 release. It has been suggested that antimethanogenic additives could be used as alternatives to antibiotics in ruminant feeds (Bodas et al., 2008).

Tanins: Many plants contain tannins. It is assumed that the biological significance of many types of tannin is related to protection against infection, insects, or animal herbivory. Tannins are polyphenolic secondary metabolites of higher plants (Khanbabaee and Ree van, 2001). The two major groups of plant polyphenolic compounds other than lignin are hydrolysable (polyesters of gallic acid and various individual sugars) and condensed tannins (polymers of flavanoids) (McSweeney et al., 2001).

Anotherdefinition is that the plant tannins as water soluble phenolic compounds with a molar mass between 300 and 3000, showing the usual phenol reactions and precipitating alkaloids, gelatins and other proteins (Khanbabaee and Ree van, 2001). Condensed tannins contained in the forages have effects on feed digestibility, depending on the quality and biological activity of the tannins (Schofield et al., 2001). Forages containing condensed tannins, which can broadly be classed as rumen modifying agents, do reduce emissions (Clark, 2009a; Clark et al., 2011). Sejian and Naqvi (2012) demonstrated that Bergenia crassifolia, Emblica officinalis, Peltiphyllum peltatum, Populus deltoids, Quercus Incana, Rheum Undulatum, Terminalia belerica, Terminalia chebula and Vaccinium vitis-idaea are some other plants containing high tannin contents and have a potential to inhibit in vitro as well as in vivo CH4 emission by the rumen microbes.

For tannin-containing plants, the antimethanogenic activity has been attributed mainly to the group of condensed tannins. Hydrolysable tannins are considered more toxic to the animal (Field et al., 1989).

Introduction of condensed tannins into diets is a likely target to reduce methane production. Tannins predominant in Calliandra calothyrus reduced nutrient degradation and might have affected methanogenesis. Therefore, tannin-rich legumes such as Calliandra calothyrsus could be used in combination with good-quality forage species in order to reduce CH4 emission from rumen fermentation (Hess et al., 2003b; Hess et al., 2004; Martin et al., 2010). Other results of Hess et al. (2006) suggest that extracted tannins as well as tannin-rich legumes can be useful in limiting CH4 emission without major losses in feeding value of the diet while very tannin-rich shrub legumes such as Calliandra calothyrsus, despite being also effective in limiting methanogenesis, are restricted in their application due to the simultaneous depression of the feeding value of the diet.

Tiemann et al. (2008) reported that the inclusion of the tannin-rich plants reduced CH4 emission per day and per unit of feed and energy intake in growing lambs by up to 24 %.

Woodward et al. (2002) investigated the effect of feeding of Sulla (Hedysarum coronarium) on CH4 emission and milk yield in dairy cows. Cows fed sulla produced less CH4 per kg DMI (19.5 vs. 24.6 g) and per kg milk solid yield (243.3 vs. 327.8 g). Tavendale et al. (2005) evaluated inhibitory effects of extractable condensed tannin fractions from L. pedunculatus on the common rumen methanogens Methanobrevibacter ruminantium. Results indicate that condensed tannin action on methanogenesis can be attributed to indirect effects via reduced hydrogen production (and presumably reduced forage digestibility) and via direct inhibitory effects on methanogens.

Waghorn et al. (2002) recorded significant difference in sheep fed diet with Lotus pedunculatus (a condensed tannin containing legume) or pasture (11.5 g DMI vs. 25.7 g DMI). Waghorn et al. (2002) observed the impact of condensed tannins on rumen methanogenesis to be small but significant; a 16 % reduction. Woodward et al. (2001) showed lower daily CH4 outputs when sheep were fed Lotus pedunculatus than ryegrass-based pasture or lucerne (14.5 vs. 20.4 vs. 19.0 g DMI). Friesian dairy cows fed either Lotus corniculatus silage had lower total CH4 outputs than cows fed perennial ryegrass silage (26.90 vs. 35.13 g DMI). The mitigation of CH4 emissions from animals fed Lotus species was due in part to a higher nutritive value relative to pasture but effects of condensed tannins on methanogenesis warrants further investigation.

Experiments with feeding of plants or extracts of condensed tannin-containing Lotus corniculatus, Lotus pedunculatus and Acacia mearnsii reduced CH4 production in small ruminants (sheep, alpaca, goats) by up to 30 % (Pinares-Patino et al., 2003ab; Puchala et al., 2005). Angora goats received the condensed tannin-containing forage Sericea lespedeza (17.7 %) or a mixture of crabgrass (Digitaria ischaemum) and Kentucky 31 tall fescue (Festuca arundinacea). Methane emission was significantly lower for Sericea lespedeza than for crabgrass with tall fescue (7.4 vs. 10.6 g.d-1; 6.9 vs. 16.2 DMI) (Puchala et al., 2005).

There are some studies about the effects of plant extracts on CH4 production both in vitro and in vivo. However, research indicated that at least some of the measured decrease in CH4 production is the result of direct effect of condensed tannins in legumes forages on rumen fermentation (Woodward, 2003; Iqbal et al., 2008). The extracts of pods of Acacia concinna, seed pulp of Terminalia chebula, Terminalia belerica, Emblica officinalis and seed kernel of Azadirachta indica were evaluated for their effect on CH4 production. The results indicate that the plant extracts appear to have a potential to manipulate rumen fermentation. The methanol extract of the seed pulp of Terminalia chebula has antimethanogenic activity and extracts of the pods of Acacia concinna and Azadirachta indica have defaunating properties (Patra et al., 2006).

The De Oliveira et al. (2007) study evaluated the effect of diets containing sorghum silages with higher and lower tannin concentrations. There was no effect of tannin levels on digestibility and CH4 emission. The lower ruminal digestibility of neutral detergent fiber in high tannin silage suggests an inhibitory effect of tannins on ruminal fiber digestibility.

Ionophores: Ionophores (monensin and lasalocid) are antimicrobials which have been extensively investigated for their ability to reduce CH4 production in ruminants. These compounds are also classed as antibiotics and their use is unacceptable in some locations. Their effect on microbes and protozoa was showed. Ionophores are highly lipophilic polyethers that accumulate in cell membranes and catalyze rapid ion movement (Russell and Houlihan, 2003). Ionophores modulate the movement of cations such as sodium, potassium and calcium across cell membranes. These chemicals also reduce acetate production, they cause a direct inhibition on H2 from rumen fermentation (Russell and Houlihan, 2003; Sejian and Naqvi, 2012). The effects of ionophores on enteric CH4 production are related to depress of ciliate protozoal populations (Iqbal et al., 2008).

Ionophores cause a shift in the rumen bacterial population from gram positive to gram negative organisms, with a concurrent shift in fermentation from acetate to propionate (Callaway et al., 1997), reaction is associated with a reduction in methanogenesis (Iqbal et al., 2008; Sejian and Naqvi, 2012).

According to Guan et al. (2006), both monensin and lasalocid decreased in beef total ciliate protozoal populations by 82.5 % in the first 2 wk and by 76.8 % in the first 4 wk after treatment, respectively. Supplementation of monensin in cattle diets can decrease enteric CH4 emissions by 27 to 30 % for 2 to 4 weeks, depending on energy content of the diet (Guan et al., 2006). Monensin addition to the diet at 24 ppm significantly decreased CH4 production compared with cows fed diets without monensin (Sauer et al., 1998). Monensin supplementation to both faunated and unfaunated goats also reduced CH4 production, though the degree of reduction in unfaunated animals was smaller than that in faunated animals (Shibata and Terada, 2010). Callaway et al. (1997) wrote that maximum CH4 inhibition by monensin was 18 % in vitro. McGinn et al. (2004) reported that monensin tended to lower CH4 emissions per kilogram of DMI by 8.6 % compared with the control in beef cattle.

Russell and Strobel (1989) indicated in vitro and in vivo experiments that monensin decreases CH4 production by 30 %, but methanogenic bacteria are not particularly sensitive to ionophores. McCaughey et al. (1997) observed no benefit from administration of monensin in term of CH4 production. The energy lost through breathing of CH4 averaged 4.5 % of gross energy intake.

Beauchemin et al. (2008) reported that strategies such as supplementation with ionophores (>24 ppm) are using by North America farmers because there is a high probability that they reduce CH4 emissions in addition to improving production efficiency. Unfortunately, the inhibitory effects of ionophores on methanogenesis may not persist over time. Omar (2004) found that CH4 suppression does not persist beyond two weeks (16 days) of ionophore treatment in beef steers. Also Iqbal et al. (2008) noted that cattle studies have shown that ionophore-induced suppression of enteric CH4 production is short lived. Methane emissions of sheep and cattle can be reduced by about 18 % through the use of ionophores in the short term but there are indications that methanogens as well as other members of the microbial population can adapt to their presence (Mathison et al., 1998)

Use of ionophores in animal feed has a significant impact on the transfer of antibiotic resistance from animals to man (Russell and Houlihan, 2003). Therefore, ionophores supplement in livestock production is not permitted in EU after the ban on growth promoters in January 2006 (Benchaar and Greathead, 2011).

Organic acids: Organic acids (malate, fumarate, acrylate, and others) have been shown to be the most effective against CH4 in vitro trials (Martin et al., 2010). Dicarboxylic organic acids (malate, fumarate) are potential precursors of propionate which stimulate H2 utilization (Song et al., 2011). They can be an alternative sink for metabolic hydrogen and reduce the amount of hydrogen used in CH4 formation (Lila et al., 2004a; McGinn et al., 2004; Newbold et al., 2005).

Feeding of dicarboxylic organic acids to improve propionate production is other mechanisms for CH4 inhibition. It was concluded that especially sodium fumarate may be a useful dietary additive for ruminants, because it diverts some H2 from CH4 production and because it is able to stimulate proliferation of cellulolytic bacteria and digestion of fibre (Lopez et al., 1999). Fumarate and acrylate to be the most effective in in vitro trials (Mohammed et al., 2004; Newbold et al., 2005). The addition of fumarate and malate increased propionate production and reduced CH4 output. The increasing dietary malic acid led to a decrease of protozoa numbers and total daily CH4 emissions by 16 % (Foley et al., 2009).

Bayaru et al. (2001) also demonstrated by in vivo studies the potential effects of dicarboxylic acids on CH4 output. These authors concluded that the dietary fumarate supplementing would have a beneficial effect via decreased methanogenesis, increased propionate production and stimulation of fibre breakdown by rumen microorganisms. The inclusion of dietary additives contained calcium fumarate decreased CH4 emissions in dairy cows by 10 % (Zijderveld van et al., 2011). According to Carro and Ranilla (2003), addition of fumarate did not affect the total gas production in in vitro trial. If experiments are done in vivo, fumarate could be used as a feed additive for ruminant animals fed high proportions of cereal grains, because it increased pH, acetate and propionate production and it decreased CH4 production (Carro and Ranilla, 2003).

The opposite of response to organic acids in vitro, responses to dietary supplementation in vivo were inconclusive and highly variable. McGinn et al. (2004) reported that fumaric acid was not effective in reducing CH4 losses of beef cattle in vivo. Newbold et al. (2002) reported a dose-dependent response to fumarate in sheep. The effect of fumaric acid - bentonite coupled addition on rumen fermentation efficiency would improve the impact of fumaric acid on rumen fermentation pattern and can be appropriate alternative for antibiotic feed additives in improving ruminants feed efficiency (Abdl-Rahman, 2010). According to Martin (1998), malate was also effective in reducing the drop in ruminal pH normally seen 1 to 2 h after feeding a high-grain diet and improved cattle performance. Therefore, supplementing finishing diets or high-producing dairy cattle diets with malate might be effective in reducing subclinical acidosis.

An exceptional decrease in CH4 production, up to 75 %, has been shown with 10 % encapsulated fumarate in the diet of lambs without negative effect on animal growth (Wallace et al., 2006; cited by Martin et al., 2010). Also in the Wood et al. (2009) study were encapsulated fumaric acids effective. In vivo, growing lambs on a concentrate diet with straw ad libitum produced 24.6 L.d-1 of CH4, whereas a 100 addition of fumaric acid encapsulated in partially hydrogenated vegetable oil or encapsulated fumaric acid decreased significantly CH4 production to 9.6 and 5.8 L.d-1, respectively. The 76 % decrease in CH4 is among the largest reported in the literature to date. In contrast, encapsulated fumarate had no significant effect in another trial in dairy cows (McCourt et al., 2008; cited by Martin et al., 2010).

Fumaric acid is a precursor of propionic acid in the fermentation of feed in the rumen and can act as an alternate sink for consumption of hydrogen generated in the rumen. The levels of fumaric acid required to inhibit methanogenesis to a significant extent may cause a drop in pH which might affect feed fermentation adversely.

Free fumaric acid (10 % in the ration) and an equivalent amount of encapsulated fumaric acid decreased CH4 emission to the extent of 49 % and 75 % compared to control sheep without supplementation of fumaric acid (Agrawal and Kamra, 2010, cited by Sejian and Naqvi, 2012).

Rumen additives have shown some success in vitro but results from in vivo trials with fumaric acid have been disappointing. Fumaric acid caused potentially beneficial changes in ruminal fermentation but no measurable reductions in CH4 emissions (Foley et al., 2009). Fumarate supplementation for an extended period may result in the amplification of small populations of fumarate-reducers (Ungerfeld et al., 2007). The inclusion of malate as a feed additive into the diets of ruminants is currently not economically feasible (Martin, 1998). The following constraints are associated with the use of dicarboxylic acids. They are expensive chemicals. They are not suitable for grazing animals as they have to be fed daily. And finally, their efficiency is reduced when concentrates are fed, as evident from in vitro trial in which the efficiency was only 4.8 % (Iqbal et al., 2008).

Lipids: Generally, lipids cause depressive effect on CH4 emission by protozoal inhibition, reduction of activity of ruminal methanogens, protozoal numbers, and double bonds in unsaturated fatty acids, and enhanced propionate production (Hristov et al., 2013b). Lipid supplementation can reduce methanogenesis without negatively affecting total digestibility and ruminal pH as opposed to concentrates (Hook et al., 2010; Sejian et al., 2011b). Pinares-Patino et al. (2016) concluded that enhanced dietary lipids contents is an effective means of reducing CH4 emissions from grazed pasture.

Fat reduces the fermentable substrate and can lower organic matter and fibre degradability. Short-term additives as dietary oils or lipids can be added to reduce CH4 emissions by decreasing fermentation in the gut and reducing the activity of the micro-organisms in the gut.

Fat supplementation can serve as an important energy source in diets of high producing ruminants; however, excessive fat addition will depress fiber degradation in the rumen (Patra, 2014).

The study of Alstrup et al. (2015) followed the effect of fat on CH4 production throughout the lactation, and showed that the mitigation effect of fat on CH4 production is persistent, and that CH4 production increases with days in milk. Moate et al. (2011) found that for each 10 DM increase in dietary fat concentration were enteric emissions reduced by 0.79 g DMI, or 3.5 %. Martin et al. (2010) reported a decrease in CH4 of 3.8 % with each 1% addition of supplemental fat. A CH4 decrease of 30 % has been observed when 12 % tallow was added to the diet (Van der Honing et al., 1983). Dietary supplementation with fat is the most promising dietary strategy, but the milk production and composition response to supplementary fat is complex and differs among diets. It is also affected by stage of lactation, degree of saturation of the added fat, amount of fat added, and the fat content and composition of the basal diet.

For cattle, a 10 g/kg increase in dietary fat decreased CH4 yield by 1 DMI, but for sheep the decrease was 2.6 DMI (Grainger and Beauchemin, 2011).

Essential oils are naturally occurring plant secondary metabolites, which exhibit antimicrobial activities against gram-negative and gram-positive bacteria, a property that has been attributed to the presence of terpenoid and phenolic compounds (Meale et al., 2014). Some essential oils, especially their organosulphur compounds, are capable to affect rumen fermentations and decrease CH4 production in vitro (Busquet et al., 2005; Macheboeuf et al., 2006; McAllister and Newbold, 2008). Allium sativum, Coriandrum sativum, Eucalyptus globules, Foeniculum vulgare, Mentha piperita, Ocimum sanctum, Populus deltoids, Juniper berry, Syzygium aromaticum, Thymus vulgaris, Origanum vulgare, Cinnamonum cassia, Rheum rhabarbarum, Armoracia rusticana, and Rhamnus frangula are some of the plants which contain high concentration of essential oils and are effective against CH4 emission and protozoa growth in the rumen (Benchaar and Greathead, 2011).

In the Busquetet al. (2005) in vitro study, treatments of garlic oil, diallyl disulfide, and allyl mercaptan resulted in a decrease in CH4 production of 73.6, 68.5, and 19.5 %, respectively, compared with the control. However, inhibition of CH4 production occurred at high doses was associated with a decrease in total volatile fatty acid concentrations and feed digestion (Benchaar and Greathead, 2011). Meale et al. (2014) demonstrated in vivo experiments that feed ration supplementation with garlic oil (5 g.d-1) and juniper berry oil (2 g.d-1) had no effects on enteric CH4 emissions in lactating dairy cows whether expressed as g.d-1, DMI, of milk or as DMI.body weight.

The positive effects of fatty acids on CH4 reduction through their toxicity to methanogens have also been demonstrated (Sejian and Naqvi, 2012). Unsaturated fatty acids serve as electron acceptors during biohydrogenation, causing this depression in CH4 production. Supplementation of fatty acids to the feed decreases CH4 production (Martin et al., 2008; Iqbal et al., 2008; Brask et al., 2013), and it would be one of the dietary strategies to abatement of CH4 production (Hristov et al., 2013a). However, the effect may not last long, it is dependent on fatty acids composition. Medium-chain fatty acids are the more depressive. The study of Beauchemin et al. (2009) shows that adding sources of long-chain unsaturated fatty acids to the diet in the form of processed oilseeds can be an effective means of reducing CH4 emissions.

Saturated acids, lauric and myrstic acid, are particulary toxic to methanogens from bovine ruminal fluid (Sejian and Naqvi, 2012). Myristic acid inhibited significantly rumen archaea without significantly altering proportions of individual methanogen orders (Machmuller et al., 2003). According to Soliva et al. (2004), these acids taken alone have similar effects, but a combination between these two acids has a clear synergistic effect leading to a sharp decrease in CH4. The most effective mixture of lauric and myristic acid, when supplemented to a complete ruminant diet, was the 4: 1 rate. It decreased CH4 release by about 70 % in vitro.

Addition of oils to ruminant diets may decrease CH4 emission by up to 80 % in vitro and about 25 % in vivo (Machmuller et al., 2000; Fievez et al., 2003; Singh, 2010).The effects of different oils on ruminal methane fermentation are related to diet composition. Infusion trials of these unsaturated acids (oleic, linoleic and linolenic) showed the greater the depression of CH4 production (Shibata and Terada, 2010). Linolenic acid has been shown to have a higher effect on CH4 than linoleic acid in vitro (Newbold et al., 1996, cited by Martin et al., 2010; Jouany et al., 2008, cited by Martin et al., 2010). In Martin et al. (2008) four weeks study they demonstrated that a 5.7 % additivum of lipids from Linum usitatissimum seed decreases the quantity of CH4 emitted daily by dairy cows, with a marked effect of the physical form of Linum usitatissimum seed.

All the forms of linseed fat (polyunsaturated) acids significantly decreased daily CH4 emissions but to different extents (-12 % with crude linseed, -38 % with extruded linseed, -64 % with linseed oil) compared with control group. The addition of dietary oil (6 % of DMI) from refined soy oil decreased (40 %) CH4 output in young bulls (Jordan et al., 2006a).

Sunflower seed which is rich in linoleic acid had a similar depressive effect as coconut oil on CH4 production, and this effect was higher than rapeseed (rich in oleic acid), and especially than linseed (with the amount oflinolenic acid) in vitro treatment (Machmuller et al., 1998). Coconut (Cocos nucifera) oil, containing mostsaturatedacids, completely eliminated protozoa from rumen fluid after four to nine days of application, and this period was shorter by more than 30 % on average at the higher level of supply. As compared with the low-fat treatment, coconut oil suppressed CH4 by 43 % with medium and 57 % with high concentrate level. The maximum CH4 reduction with sunflower seed and lin seed accounted for about 40 % (Machmuller et al., 1998).

McGinn et al. (2004) observed a lower acetate concentrations, higher propionate concentrations and lower acetate to propionate ratio when sunflower oil was added to the diets of cattle. They reported a 22 % decrease in CH4 emissions by addition of sunflower oil to the diet of cattle.

Beauchemin et al. (2009) added crushed sunflower seeds, crushed flaxseed, and crushed canola seed to the diets of lactating dairy cows (supplying 3.7 % added fat). All 3 oilseed treatments reduced daily CH4 production (g.d-1) by an average of 13 % compared with a control diet containing calcium salts of palm fat acid (long-chain fatty acid). However, the abatement effect of fat acids supplementation on CH4 production was not observed in other studies on dairy cows. According to Johnson et al. (2002), supplementation of diets with oilseeds did not affect CH4 emissions but tended to increase the efficiency of milk produced per unit of methane emitted. Cosgrove et al. (2008) infused linseed and sunflower oils (3: 1) directly into the rumen of 8 month old wether sheep. Sheep tolerated additional oil up to 5 % of DMI, but when 6.2 % was given, feed intakes declined and this treatment was discontinued. Up to 5 % oil infusion did not affect CH4 production.

Machmuller et al. (2000) evaluated on growing lambs diets consisted of maize silage, grass hay and concentrate which was supplemented with the respective lipid source. On average, the five lipid-supplemented diets contained 56 g ether extract per kg dry mater. Coconut oil (medium-chain fatty acids) supplementation reduced significantly CH4 release per kg live weight by 26 % compared to control, and with the use of rapeseed, sunflower, and linseed seed the relative reduction was 19 %, 27 % and 10 %, respectively. At the study of Kumar et al. (2007) eight feeds (mixture of wheat straw and oil seed cakes in 3: 1 ratio) were evaluated for CH4 emission and fermentation pattern with buffalo rumen liquor in an in vitro gas production test. The cakes tested were groundnut, soybean, mustard, cotton, karanj, and caster bean. Among the oil cakes, mustard seed cake-based feed produced the minimum CH4 without affecting other fermentation characteristics adversely.

The efficiency of different oils is also different. Machmuller et al. (1998) identified coconut oil as more effective inhibitor than rape seed (canola, rich in oleic acid), sunflower seed (rich in linoleic acid), and linseed oil (rich in both linoleic and linolenic acid). They suggested that coconut oil eliminated protozoa from rumen fluid after four to nine days of application and directly inhibited rumen methanogens, probably by changing their metabolic activity and composition. As compared with the low-fat treatment, coconut oil suppressed methane by 43 % with medium and 57 % with high concentrate level. The methane suppressive effect of 6 % and 9 % total dietary fat was about 40 % and 60 % with coconut oil and about 20 % and 40 % with whole crushed oilseeds rich in polyenoic acids, respectively.

Conclusions: Decreasing CH4 emissions has become a priority and an integral part of climate control. This review has identified a number of techniques that will result in reduced CH4 emissions when implemented at a commercial scale. However, the development of more methods is at an early stage of work. Also, the cost of some strategies is likely to be too expensive for commercial husbandry.

More chemicals are used to reduce the formation of CH4. Direct inhibition of CH4 generation, vaccination, and probiotics are the promising approaches for future research. But, the use currently available strategies of defaunation are not a permanent solution of ruminant CH4 due to microbial adaptation. Developing vaccines which stimulate ruminants to produce antibodies against their rumen methanogens may be feasible in principle but the successful development of a vaccine is still a long way off.

Addition of halogenated methane analogues to ruminant diet offers the opportunity to reduce CH4 production in cattle but rumen microbes can adapt to them. The use of probiotics remains a potentially interesting, but results have been either unsatisfactory, not conclusive, or have yet to be confirmed in vivo.

In case of ionophores, many chemical compounds have been tested as additives for ruminants. The monensin has been found to reduce enteric CH4 emissions. However, the effects are variable and the inhibitory effects on methanogenesis may not persist over time. Also, use of ionophores in livestock production is not permitted in the EU after the ban on growth promoters. Prophylactic effects of miscellaneous compounds should be developed instead of ionosphores.

Most positive reports concern the fat, essential oils, tannins, saponins, and plant extracts. Some essential oils may exert a direct effect on methanogens. Polyunsaturated fatty acids contribute to CH4 decrease through a toxic effect on cellulolytic bacteria and protozoa. Dietary fat seems a promising nutritional alternative to depress ruminal methanogenesis without affecting other ruminal parameters. Dietary lipids can be effective in reducing CH4 emissions, but their applicability will depend on effects on feed intake, fiber digestibility, production, and milk composition. This review has shown that addition of fat to the diet can result in a persistent decrease in CH4 emissions, and not lower animal production. However, the addition of oils to feed may depress dry matter intake and fiber digestibility.

The response to tannin and saponin is highly variable and more research is needed to assess the effectiveness and eventual presence of undesirable residues in animal products.

Possible effects include also plant extracts and improved yeast products. The use of plant extracts is receiving a renewed interest as they are seen as a natural alternative to chemical additives and are well perceived by consumers. However, a limited number of studies have investigated, and it is difficult to provide a comprehensive assessment at this stage about the size of decrease that might be realistically expected in vivo.

In summary, this study has shown that CH4 abatement can be possible. The several interventions for reducing CH4 emission by ruminants were described. However, there is a need for in vivo investigation of proposed manners. Practical methods of reducing protozoa in the rumen are required.

Acknowledgements: This article was possible through projects APVV of the Slovak Research and Development Agency Bratislava (0632-10 and 15-0060), also by the projects AGB 313011 and CEGEZ 26220120073 supported by the Operational Programme Research and Development funded from the European Regional Development Fund.


Abdl-Rahman, M. A. (2010). In vitro Manipulation of Rumen Fermentation Efficiency by Fumaric acid - Bentonite Coupled Addition as an Alternative to Antibiotics. J. Agric. Sci. 2: 174-180.

Agrawal, D.K. and D.N. Kamra (2010). Global warming: Role of livestock and mitigation strategies, in: Int. Conf. on "Physiological capacity building in livestock under changing climate scenario". Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India, 27-39. Cited by Sejian and Naqvi, 2012.

Alstrup, L., A.L. Frydendahl Hellwing, P. Lund, and M.R. Weisbjerg (2015). Effect of fat supplementation and stage of lactation on methane production in dairy cows. Anim. Feed Sci. Technol. 207: 10-19.

Arif, M., M. Sarwar, Mehr-un-Nisa, Z. Hayat, and M. Younas (2016). Effect of supplementary sodium nitrate and sulphur on methane production and growth rates in sheep and goats fed forage based diet low in true protein. The J. Anim. Plant Sci. 26: 69-78.

Baker, S.K., G. Gnanasampanthan, D.B. Purser, and R.M. Hoskinson (1997). Immunogenic preparation and method for improving the productivity of ruminant animals. Patent application. WO 97/00086. Cited by Iqbal et al., 2008.

Bauchop, T. (1967). Inhibition of Rumen Methanogenesis by Methane Analogues. J. Bacteriol. 94: 171-175.

Bayaru, E., S. Kanda, T. Kamada, H. Itabashi, S. Andoh, T. Nishida, M. Ishida, T. Itoh, K. Nagara, and Y. Isobe (2001). Effect of fumaric acid on methane production, rumen fermentation, and digestibility of cattle fed roughage alone. Anim. Sci. J. 72: 139-146.

Beauchemin, K.A., M. Kreuzer, F. O'Mara, and T.A. McAllister (2008). Nutritional management for enteric methane abatement: a review. Aust. J. Exp. Agric. 48: 21-27.

Beauchemin, K.A., T.A. McAllister, and S.M. McGinn (2009a). Dietary mitigation of enteric methane from cattle. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 4 (035): 1-18.

Beauchemin, K.A., S.M. McGinn, C. Benchaar, and L. Holtshausen (2009b). Crushed sunflower, flax, or canola seeds in lactating dairy cow diets: effects on methane production, rumen fermentation, and milk production. J. Dairy Sci. 92: 2118-2127.

Benchaar, C. and H. Greathead (2011). Essential oils and opportunities to mitigate enteric methane emissions from ruminants. Anim. Feed Sci. Technol. 166-167: 338-355.

Bodas, R., S. Lopez, M. Fernandez, R. Garcia-Gonzalez, A.B. Rodriguez, R.J. Wallace, and J.S. Gonzalez (2008). In vitro screening of the potential of numerous plant species as antimethanogenic feed additives for ruminants. Anim. Feed Sci. Technol. 145: 245-258.

Brask, M., P. Lund, M.R. Weisbjerg, A.L.F. Hellwing, M. Poulsen, M.K. Larsen, and T. Hvelplund (2013). Methane production and digestion of different physical forms of rapeseed as fat supplements in dairy cow. J. Dairy Sci. 96: 2356-2365.

Broucek, J. (2015). Methane yield from cattle, sheep, and goats housing with emphasis on emission factors: a review. Slovak J. Anim. Sci. 48: 122-139.

Busquet, M., S. Calsamiglia, A. Ferret, M.D. Carro, and C. Kamel (2005). Effect of garlic oil and four of its compounds on rumen microbial fermentation. J. Dairy Sci. 88: 4393-4404.

Callaway, T.R., A.M. Carneiro De Melo, and J.B. Russell (1997). The effect of nisin and monensin on ruminal fermentations in vitro. Curr. Microbiol. 35: 90-96.

Carro, M.D. and M.J. Ranilla (2003). Influence of different concentrations of disodium fumarate on methane production and fermentation of concentrate feeds by rumen micro-organisms in vitro. Br. J. Nutr. 90: 617-624.

Clark, H. (2009). Methane emissions from ruminant livestock; are they important and can we reduce them? Proc. New Zeal. Grassland Assoc. 71: 73-76.

Clark, H., F. Kelliher, and C. Pinares-Patino (2011). Reducing CH4 Emissions from Grazing Ruminants in New Zealand: Challenges and Opportunities. Asian-Aust. J. Anim. Sci. 24: 295-302.

Cook, S.R., P.K. Maiti, A.V. Chaves, C. Benchaar, K.A. Beauchemin, and T.A. McAllister (2008). Avian (IgY) anti-methanogen antibodies for reducing ruminal methane production: in vitro assessment of their effects. Austral. J. Experim. Agric. 48: 260-264.

Cosgrove, G.P., G.C. Waghorn, C.B. Anderson, J.S. Peters, A. Smith, G. Molano, and M. Deighton (2008). The effect of oils fed to sheep on methane production and digestion of ryegrass pasture. Austral. J. Experim. Agric. 48: 189-192.

Cottle, D.J., J.V. Nolan, and S.G. Wiedemann (2011). Ruminant enteric methane mitigation: a review. Anim. Prod. Sci.51: 491-514.

Cottle, D. and R. Eckard (2014). Modelling the reduction in enteric methane from voluntary intake versus controlled individual animal intake of lipid or nitrate supplements.Anim. Prod. Sci. 54: 2121-2131.

Chaucheyras, F., G. Fonty, G. Bertin, and P. Gouet (1995). In-vitro H2 utilisation by a ruminal acetogenic bacterium cultivated alone or in association with an Archea methanogen is stimulated by a probiotic strain of Saccharomyces cerevisiae. Appl. Environ. Microbiol. 61: 3466-3467.

Chaucheyras-Durand, F., N.D. Walker, and A. Bach (2008). Effects of active dry yeasts on the rumen microbial ecosystem: past, present and future. Anim. Feed Sci. Technol. 145: 5-26.

Dammgen, U., C. Rosemann, H.D. Haenel, and N.J. Hutchings (2012). Enteric methane emissions from German dairy cows. Landbauforsch. vTI Ag. For. Res. 62: 21-32.

Das, T.K., D. Banerjee, D. Chakraborty, M.C. Pakhira, B. Shrivastava, R.C. Kuhad (2012). Saponin: Role in Animal system. Vet. World. 5: 248-254.

De Oliveira, S.G., T.T. Berchielli, M.D. Pedreira, O. Primavesi, R. Frighetto, and M.A. Lima (2007). Effect of tannin levels in sorghum silage and concentrate supplementation on apparent digestibility and methane emission in beef cattle. Anim. Feed Sci. Technol. 135: 236-248.

Dohme, F., A. Machmueller, B.L. Estermann, P. Pfister, A. Wasserfallen, and M. Kreuzer (1999). The role of rumen ciliate protozoa for methane suppression caused by coconut oil. Lett. Appl. Microbiol. 29: 187-192.

Doreau, M. and J.P. Jouany (1998). Effect of a Saccharomyces cerevisiae culture on nutrient digestion in lactating dairy cows. J. Dairy Sci. 81: 3214-3221.

Dumitru, R., H. Palencia, S.D. Schroeder, B.A. Takacs, M.E. Rasche, J.L. Miner, and S.W. Ragsdale (2003). Targeting methanopterin biosynthesis to inhibit methanogenesis. Appl. Environ. Microbiol. 69: 7236-7241.

Eckard, R.J., C. Grainger, and C.A.M. de Klein (2010). Options for the abatement of methane and nitrous oxide from ruminant production: a review. Livest. Sci.130: 47-56.

Field, J.A., S. Kortekaas, and G. Lettinga (1989). The tannin theory of methanogenic toxicity. Biol. Wastes. 29: 241-262.

Fievez, V., F. Dohme, M. Danneels, K. Raes, and D. Demeyer (2003). Fish oils as potent rumen methane inhibitors and associated effects on rumen fermentation in vitro andin vivo. Anim. Feed Sci. Technol. 104: 41-58.

Finlay, B.J., G. Esteban, K.J. Clarke, A.G. Williams, T.M. Embley, and R.P. Hirt (1994). Some rumen ciliates have endosymbiotic methanogens. FEMS Microbiol. Lett. 117: 157-162.

Finn, D., R. Dalal, and A. Klieve (2015). Methane in Australian agriculture: current emissions, sources and sinks, and potential mitigation strategies. Crop Pasture Sci. 66: 1-22.

Flachowsky, G. and W. Brade (2007). Potenziale zur Reduzierung der Methan-Emissionen bei Wiederkauern. Zkunde. 79: 417-465.

Foley, P.A., D.A. Kenny, J.J. Callan, T.M. Boland, and F.P. O'Mara (2009). Effect of DLmalic acid supplementation on feed intake, methane emission, and rumen fermentation in beef cattle. J. Anim. Sci. 87: 1048-1057.

Goel, G., H.P.S. Makkar, and K. Becker (2008a). Changes in microbial community structure, methanogenesis and rumen fermentation in response to saponin-rich fractions from different plant materials. J. Appl. Microbiol. 105: 770-777.

Goel, G., H.P.S. Makkar, and K. Becker (2008b). Effects of Sesbania sesban and Carduus pycnocephalus leaves and Fenugreek (Trigonella foenum-graecum L.) seeds and their extracts on partitioning of nutrients from roughage-and concentrate-based feeds to methane. Anim. Feed Sci. Technol. 147: 72-89.

Grainger, C. and K.A. Beauchemin (2011). Can enteric methane emissions from ruminants be lowered without lowering their production? Anim. Feed Sci. Technol. 166-167: 308-320.

Guan, H., K.M. Wittenberg, K.H. Ominski, and D.O. Krause (2006). Efficacy of ionophores in cattle diets for mitigation of enteric methane. J. Anim. Sci. 84: 1896-1906.

Guerci, M., L. Bava, M. Zucali, A. Sandrucci, Ch. Penatiand, and A. Tamburini (2013). Effect of farming strategies on environmental impact of intensive dairy farms in Italy. J. Dairy Res. 80: 300-308.

Hegarty, R.S. (1999). Reducing rumen methane emissions through elimination of rumen protozoa. Aust. J. Agric. Res. 50: 1321-1327.

Hess, H.D., M. Kreuzer, T.E. Diaz, C.E. Lascano, J.E. Carulla, C.R. Soliva, and A. Machmuller (2003a). Saponin rich tropical fruits affect fermentation and methanogenesis in faunated and defaunated rumen fluid. Anim. Feed Sci. Technol. 109: 79-94.

Hess, H.D., L.M. Monsalve, C.E. Lascano, J.E. Carulla, T.E. Diaz, and M. Kreuzer (2003b). Supplementation of a tropical grass diet with forage legumes and Sapindus saponaria fruits: effect on in vitro ruminal nitrogen turnover and methanogenesis.Aust. J. Agric. Res. 54: 703-713.

Hess, H.D., R.A. Beuret, M. Lotscher, I.K. Hindrichsen, A. Machmuller, J.E. Carulla, C.E. Lascano, and M. Kreuzer (2004). Ruminal fermentation, methanogenesis and nitrogen utilization of sheep receiving tropical grass hay-concentrate diets offered with Sapindus saponaria fruits and Cratylia argentea foliage. Anim. Sci. 79: 177-189.

Hess, H.D., T.T. Tiemann, F. Noto, J.E. Carulla, and M. Kreuzer (2006). Strategic use of tannins as means to limit methane emission from ruminant livestock. Int. Congr. Ser. 1293: 164-167.

Holtshausen, L., A.V. Chaves, K.A. Beauchemin, S.M. McGinn, T.A. McAllister, N.E. Odongo, P.R. Cheeke, and C. Benchaar (2009). Feeding saponin-containing Yucca schidigera and Quillaja saponaria to decrease enteric methane production in dairy cows. J. Dairy Sci. 92: 2809-2821.

Hook, S.E., A.D.G. Wright, and B.V. McBride (2010). Methanogens: Methane Producers of the Rumen and Mitigation Strategies. Archaea, Article ID 945785, 11 pages. http: //

Hristov, A.N., J. Oh, J. Firkins, J. Dijkstra, E. Kebreab, G. Waghorn, H.P.S. Makkar, A.T. Adesogan, W. Yang, C. Lee, P.J. Gerber, B. Henderson, and J.M. Tricarico (2013a). Mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options. J. Anim Sci. 91: 5045-5069.

Hristov, A.N., T. Ott, J. Tricarico, A. Rotz, G. Waghorn, A.T. Adesogan, J. Dijkstra, F. Montes, J. Oh, E. Kebreab, S.J. Oosting, P.J. Gerber, B. Henderson, H.P.S. Makkar, and J. Firkins (2013b). Mitigation of methane and nitrous oxide emissions from animal operations: III. A review of animal management mitigation options. J. Anim Sci. 91: 5095-5113.

Hristov, A.N., J. Oh, F. Giallongo, T.W. Frederick, M.T. Harper, H.L. Weeks, A.F. Branco, P.J. Moate, M.H. Deighton, S.R.O Williams, M. Kindermann, S. Duval (2015). An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production. PNAS. 112: 10663-10668.

Hu, W.L., J.X. Liu, J.A. Ye, Y.M. Wu, Y.Q. Guo (2005). Effect of tea saponin on rumen fermentation in vitro. Anim. Feed Sci. Technol. 120: 333-339.

Iqbal, M.F., Y.F. Cheng, W.Y. Zhu, and B. Zeshan (2008). Mitigation of ruminant methane production: current strategies, constraints and future options. World J. Microbiol. Biotechnol. 24: 2747-2755.

Janssen, P.H. and M. Kirs (2008). Structure of the archaeal community of the rumen. Appl. Environ. Microbiol. 74: 3619-3625.

Joblin, K.N. (1999). Ruminal acetogens and their potential to lower ruminant methane emissions. Aust. J. Agric. Res. 50: 1307-1313.

Johnson, K.A., R.L. Kincaid, H.H. Westberg, C.T. Gaskins, B.K. Lamb, and J.D. Cronrath (2002). The effect of oilseeds in diets of lactating cows on milk production and methane emissions. J. Dairy Sci. 85: 1509-1515.

Jordan, E., D. Kenny, M. Hawkins, R. Malone, D.K. Lovett, and F.P. O'Mara (2006). Effect of refined soy oil or whole soybeans on intake, methane output, and performance of young bulls. J. Anim. Sci. 84: 2418-2425.

Jouany, J.P., Y. Papon, D.P. Morgavi, and M. Doreau (2008). Linseed oil and a combination of sunflower oil and malic acid decrease rumen methane emissions in vitro. In: Livestock and global climate change (eds. P. Rowlinson, M. Steele and A. Nefzaoui), Cambridge University Press, Cambridge, UK, 140-143. Cited by Martin et al., 2010.

Khanbabaee, K. and T. van Ree (2001). Tannins: Classification and Definition. Nat. Prod. Rep.18: 641-649.

Kirchgessner, M., W. Windisch, H.L. Muller, and M. Kreuzer (1991). Release of methane and of carbon dioxide by dairy cattle. Agribiol. Res. 44: 91-102.

Kumar, R., D.N. Kamra, N. Agarwal, and L.C. Chaudhary (2007). In vitro methanogenesis and fermentation of feeds containing oil seed cakes with rumen liquor of buffalo. Asian-Aust. J. Anim. Sci. 20: 1196-1200.

Kurihara, M., S. Kume, T. Aii, S. Takahashi, M. Shibata, and T. Nishida (1995). Feeding method for dairy cattle to cope with global warming - Technical assessment based on energy metabolism. The Bull. Kyushu Natl. Agr. Exp. Stat. 29: 21-107.

Kurihara, M., T. Magner, R.A. Hunter, and G.J. McCrabb (1999). Methane production and energy partition of cattle in the tropics. Br. J. Nutr. 81: 227-234.

Lanigan, G.W. (1972). Metabolism of pyrrolizidine alkaloids in the ovine rumen. IV. Effects of chloral hydrate and halogenated methanes on rumen methanogenesis and alkaloid metabolism in fistulated sheep. Aust. J. Agric. Res.23: 1085-1091.

Lee, S.S., J.T. Hsu, H.C. Mantovani, and J.B. Russell (2002). The effect of bovicin HC5, a bacteriocin from Streptococcus bovis HC5, on ruminal methane production in vitro.FEMS Microbiol. Lett.217: 51-55.

Lee, S.Y., S.H. Yang, W.S. Lee, H.S. Kim, D.E. Shin, and J.K. Ha (2009). Effect of 2-bromoethanesulfonic acid on in vitro fermentation characteristics and methanogen population. Asian-Aust. J. Anim. Sci. 22: 42-48.

Lila, Z.A., N. Mohammed, T. Yasui, Y. Kurokawa, S. Kanda, and H. Itabashi (2004). Effects of a twin strain of Saccharomyces cerevisiae live cells on mixed ruminal microorganism fermentation in vitro. J. Anim. Sci. 82: 1847-1854.

Lima, J.R., A.O. Ribon, J.B. Russell, and H. Mantovani (2009). Bovicin HC5 inhibits wasteful amino acid degradation by mixed ruminal bacteria in-vitro. FEMS Microbiol. Lett. 292: 78 - 84.

Liu, Y. and W.B. Whitman (2008). Metabolic, phylogenetic, and ecological diversity of the methanogenic Archaea. Ann. N. Y. Acad. Sci. 1125: 171-189.

Lopez, S., C. Valdez, C.J. Newbold, and R.J. Wallace (1999). Influence of sodium fumarate on rumen fermentation in vitro. Br. J. Nutr. 81: 59-64.

Macheboeuf, D., M.J. Ranilla, M.D. Carro, and D. Morgavi (2006). Combination of feed additives to meet production and environmental targets in ruminants - in vitro optimization. In: Proc. the Fifth Joint RRI-INRA Gastrointest. Tract Microbiol. Symposium, Ser. Reprod. Nutr. Develop., 46, Aberdeen, UK, 21-23 June, EDP Sci., 103-104.

Machmuller, A., D.A. Ossowski, M. Wanner, and M. Kreuzer (1998). Potential of various fatty feeds to reduce methane release from rumen fermentation in vitro (Rusitec). Anim. Feed Sci. Technol. 71: 117-130.

Machmuller, A., D.A. Ossowski, and M. Kreuzer (2000). Comparative evaluation of the effects of coconut oil, oilseeds and crystalline fat on methane release, digestion and energy balance in lambs. Anim. Feed Sci. Technol. 85: 41-60.

Machmuller, A., C.R. Soliva, M. Kreuzer (2003). Methane-suppressing effect of myristic acid in sheep as affected by dietary calcium and forage proportion. Br. J. Nutr. 90: 529-540.

Martin, S.A. (1998). Manipulation of ruminal fermentation with organic acids: a review. J. Anim. Sci. 76: 3123-3132.

Martin, C., J. Rouel, J.P. Jouany, M. Doreau, and Y. Chilliard (2008). Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed, or linseed oil. J. Anim. Sci. 86: 2642-2650.

Martin, C., D.P. Morgavi, and M. Doreau (2010). Methane mitigation in ruminants: from microbe to the farm scale. Animal. 4: 351-365.

Mathison, G.W., E.K. Okine, T.A. McAllister, Y. Dong, J. Galbraith, and O.I. Dmytruk (1998). Reducing methane emissions from ruminant animals. J. Appl. Anim. Res. 14: 1-28.

Mazzetto, A.M., A.S. Barneze, B.J. Feigl, J.W. VanGroenigen, O. Oenema, and C.C. Cerri (2014). Temperature and moisture affect methane and nitrous oxide emission from bovine manure patches in tropical conditions. Soil Biol. Biochem. 76: 242-248.

McAllister, T.A. and C.J. Newbold (2008). Redirecting rumen fermentation to reduce methanogenesis. Anim. Prod. Sci. 48: 7-13.

McCaughey, W.P., K. Wittenberg, and D. Corrigan (1997). Methane production by steers on pasture. Can. J. Anim. Sci. 77: 519-524.

McCourt, A.R., T. Yan, S. Mayne, and J. Wallace (2008). Effect of dietary inclusion of encapsulated fumaric acid on methane production from grazing dairy cows. In: Proc. of the Br. Soc. Anim. Sci., 31 March-2 April, Scarborough, UK, p. 64. Cited by Martin et al., 2010.

McGinn, S.M., K.A. Beauchemin, T. Coates, and D. Colombatto (2004). Methane emissions from beef cattle: effects of monensin, sunflower oil, enzymes, yeast, and fumaric acid. J. Anim. Sci. 82: 3346-3356.

McCrabb, G.J., K.T. Berger, T. Magner, C. May, and R.A. Hunter (1997). Inhibiting methane production in Brahman cattle by dietary supplementation with a new compound and the effects of growth. Aust. J. Agric. Res. 48: 323-329.

McSweeney, C.S., B. Palmer, D.M. McNeill, and D.O. Krause (2001). Microbial interactions with tannins: nutritional consequences for ruminants. Anim. Feed Sci. Technol. 91: 83-93.

Meale, S.J., A.V. Chaves, T.A. McAllister, A.D. Iwaasa, W.Z. Yang, and C. Benchaar (2014). Including essential oils in lactating dairy cow diets: effects on methane emissions. Anim. Prod. Sci.54: 1215-1218.

Mihina, S., V. Kazimirova, V., and T.A. Copland (2012). Technology for farm animal husbandry. 1st Issue. Slovak Agricultural University, Nitra (Slovakia). 99 p

Moate, P.J., S.R.O. Williams, C. Grainger, M.C. Hannah, E.N. Ponnampalam, and R.J. Eckard (2011). Influence of cold-pressed canola, brewers grains and hominy meal as dietary supplements suitable for reducing enteric methane emissions from lactating dairy cows. Anim. Feed Sci. Technol. 166-167: 254-264.

Mohammed, R., S.M. McGinn, and K.A. Beauchemin (2011). Prediction of enteric methane output from milk fatty acid concentrations and rumen fermentation parameters in dairy cows fed sunflower, flax, or canola seeds. J. Dairy Sci. 94: 6057-6068.

Morgavi, D.P., J.P. Jouany, and C. Martin (2008). Changes in methane emission and rumen fermentation parameters induced by refaunation in sheep. Austral. J. Experim. Agric. 48: 69-72.

Moss, A.R. (1994). Methane production by ruminants - Literature review of I. Dietary manipulation to reduce methane production, and II. Laboratory procedures for estimating methane potential of diets. Nutr. Abstr. Rev. Ser. B64: 786-806.

Mwenya, B., S.C. Santosa, Y. Gamo, T. Kobayashi, J. Takahashi (2004). Effects of including beta 1-4 galacto-oligosaccharides, lactic acid bacteria or yeast culture on methanogenesis as well as energy and nitrogen metabolism in sheep. Anim. Feed Sci. Technol. 115: 313-326.

Newbold, C.J., B. Lassalas, and J.P. Jouany (1995a). The importance of methanogens associated with ciliate protozoa in ruminal methane production in vitro. Lett. Appl. Microbiol. 21: 230-234.

Newbold, C.J., R.J. Wallace, X.B. Chen, and F.M. McIntosh (1995b). Different strains of Saccharomyces cerevisiae differ in their effects on ruminal bacterial numbers in-vitro and in sheep. J. Anim. Sci. 73: 1811-1818.

Newbold, C.J., R.J. Wallace, and F.M. McIntosh (1996). Mode of action of the yeast Sacchavomyces cerevisiae as a feed additive for ruminants. Br. J.Nutr. 76: 249-261.

Newbold, C.J., F.M. McIntosh, and R.J. Wallace (1998). Changes in the microbial population of a rumen simulating fermenter in response to yeast culture. Can. J. Anim. Sci. 78: 241-244.

Newbold, C.J., S. Lopez, N. Nelson, J.O. Ouda, R.J. Wallace, and A.R. Moss (2005). Propionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal fermentation in vitro. Br. J. Nutr. 94: 27-35.

Omar, J.A. (2004). Effect of different ionophore treatments on some rumen metabolic measures of steers. Dirasat Agric. Sci. 31: 178-184.

Patel, S., R. Shukla, and A. Goyal (2015). Probiotics in valorization of innate imunity across various animal models. J. Funct. Foods. 14: 549-561.

Patra, A.K., D. Kamra, and N. Agarwal (2006). Effect of plant extract on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Anim. Feed Sci. Technol. 128: 276-291.

Patra, A.K. (2014). A meta-analysis of the effect of dietary fat on enteric methane production, digestibility and rumen fermentation in sheep, and a comparison of these responses between cattle and sheep. Livest. Sci. 162: 97-103.

Pen, B., C. Sar, B. Mwenya, K. Kuwaki, R. Morikawa, and J. Takahashi (2006). Effects of Yucca schidigera and Quillaja saponaria extracts on in vitro ruminal fermentation and methane emission. Anim. Feed Sci. Technol. 129: 175-186.

Pinares-Patino, C.S., M.J. Ulyatt, G.C. Waghorn, K.R. Lassey, T.N. Barry, C.W. Holmes, and D.E. Johnson (2003a). Methane emission by alpaca and sheep fed on lucerne hay or grazed on pastures of perennial ryegrass/white clover or birdsfoot trefoil. J. Agric. Sci. 140: 215-226.

Pinares-Patino, C.S, M.J. Ulyatt, K.R. Lassey, T.N. Barry, and C.W. Holmes (2003b). Persistence of differences between sheep in methane emission under generous grazing conditions. J. Agric. Sci. 140: 227-233.

Pinares-Patino, C.S., F.E. Franco, G. Molano, H. Kjestrup, E. Sandoval, S. MacLean, M. Battistotti, J. Koolaard, and J. Laubach (2016). Feed intake and methane emissions from cattle grazing pasture sprayed with canola oil. Livest. Sci. 184: 7-12.

Puchala, R., B.R. Min, A.L. Goetsch, and T. Sahlu (2005). The effect of a condensed tannincontaining forage on methane emission by goats. J. Anim. Sci. 83: 182-186.

Renuka, A.K., S.A. Punia, P. Srinivasulu, T.S. Goud, A.K. Singh, L. Sharma, S. Saini, R. Devi, A. Kumar, S.V. Singh, and R.C. Upadhyay (2013). Controlling Methane Emissions from Ruminants Employing Bacteriocin. Climate Resilient Livestock and Production System, December 2013, Chapter 13, 140-153.

Russell, J.B. and H.I. Strobel (1989). Mini-Review: The effect of ionophores on ruminal fermentation. Appl. Environ. Microbiol. 55: 1-6.

Russell, J. and A. Houlihan (2003). Ionophore resistance of ruminal bacteria and its potenial impact on human health. FEMS Microbiol. Rev. 27: 65-74.

Santoso, B., B. Mwenya, C. Sar, Y. Gamo, T. Kobayashi, R. Morikawa, K. Kimura, H. Mizukoshi, and J. Takahashi (2004). Effects of supplementing galactooligosaccharides. Yucca schidigera or nisin on rumen methanogenesis, nitrogen and energy metabolism in sheep. Livest. Prod. Sci. 91: 209-217.

Santoso, B., B. Mwenya, C. Sar, and J. Takahashi (2006). Ruminal fermentation and nitrogen metabolism in sheep fed a silage-based diet supplemented with Yucca schidigera or Y. Schidigera and nisin. Anim. Feed Sci. Technol. 129: 187-195.

Sauer, F.D., V. Fellner, R. Kinsman, J.K.G. Kramer, H.A. Jackson, A.J. Lee, and S. Chen (1998). Methane output and lactation response in Holstein cattle with monensin or unsaturated fat added to the diet. J. Anim. Sci. 76: 906-914.

Schofield, P., D.M. Mbugua, and A.N. Pell (2001). Analysis of condenzed tannins: a review. Anim. Feed Sci. Technol. 91: 21-40.

Sejian, V., R. Lal, J. Lakritz, and T. Ezeji (2011). Measurement and prediction of enteric methane emission. Int. J. Biometeorol. 55: 1-16.

Sejian, V. and S.M. Naqvi (2012). Livestock and Climate Change: Mitigation Strategies to Reduce Methane Production. In: Greenhouse Gases - Capturing, Utilization and Reduction, Guoxiang Liu (Ed.), Chapter 11, 255-276. InTech, DOI: 10.5772/32014. Available from: http: //

Shibata, M. and F. Terada (2010). Factors affecting methane production and mitigation in ruminants. Anim. Sci. J.81: 2-10.

Singh, B. (2010). Some nutritional strategies for mitigation of methane emissions.In: Int. Conf. "Physiological capacity building in livestock under changing climate scenario". Physiology and Climatology division, Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India, 11-13 November, 142-158. Cited by Sejian and Naqvi, 2012.

Soliva, C.R., L. Meile, A. Cieslak, M. Kreuzer, and A. Machmuller (2004). Rumen simulation technique study on the interactions of dietary lauric and myristic acid supplementation in suppressing ruminal methanogenesis. Br. J. Nutr. 92: 689-700.

Song, M. K., Li, X. Z., Oh, Y.K., Lee, C. K., and Hyun, Y. (2011). Control of Methane Emission in Ruminants and Industrial Application of Biogas from Livestock Manure in Korea. Asian-Aust. J. Anim. Sci. 24: 130-136.

Tavendale, M. H., L.P. Meagher, D. Pacheco, N. Walker, G.T. Attwood, and S. Sivakumaran (2005). Methane production from in vitro rumen incubations with Lotus pedunculatus and Medicago sativa, and effects of extractable condensed tannin fractions on methanogenesis. Anim. Feed Sci. Technol. 123-124: 403-419.

Teferedegne, B., F. McIntosh, P.O. Osuji, A. Odenyo, R.J. Wallace, and C.J. Newbold (1999). Influence of foliage from different accessions of the sub-tropical leguminous tree. Sesbania sesban, on ruminal protozoa in Ethiopia and Scottish sheep. Anim. Feed Sci. Technol. 78: 11-20.

Thiel, P.G. (1969). The effect of methane analogues on methanogenesis in anaerobic digestion. Water Res. 3: 215-223.

Tiemann, T. T., C.E. Lascano, H.R. Wettstein, A.C. Mayer, M. Kreuzer, and H.D. Hess (2008). Effect of the tropical tannin-rich shrub legumes Calliandra calothyrsus and Flemingia macrophylla on methane emission and nitrogen and energy balance in growing lambs. Animal. 2: 790-799.

Ungerfeld, E.M., R.A. Kohn, R.J. Wallace, and C.J. Newbold (2007). A meta-analysis of fumarate effects on methane production in ruminal batch cultures. J. Anim. Sci. 85: 2556-2563.

Van der Honing, Y., S. Tamminga, B.J. Wieman, A. Steg, B. van Donselaar, and L.G.M. van Gils (1983). Further studies on the effect of fat supplementation of concentrates fed to lactating cows. 2. Total digestion and energy utilization. Neth. J. Agric. Sci. 31: 27-36.

Van Middelaar, C.E., P.B.M. Berentsen, J. Dijkstra, and I.J.M. De Boer (2013). Evaluation of a feeding strategy to reduce greenhouse gas emissions from dairy farming: The level of analysis matters. Agric. Syst.121: 9-22.

Van Nevel, C.J. and D.I. Demeyer (1996). Control of rumen methanogenesis. Environ. Monit. Assess. 42: 73-97.

Vincken, J.P., L. Heng, A. de Groot, and H. Gruppen (2007). Saponins, classification and occurrence in the plant kingdom. Phytochem. 68: 275-297.

Waghorn, G.C., M. Tavendale, and D.R. Woodfield (2002). Methanogenesis from forages fed to sheep. Proc. New Zeal. Grassland Assoc. 64: 167-171.

Wallace, R.J. and C.J. Newbold (1993). Rumen fermentation and its manipulation: the development of yeast cultures as feed additives. In: Lyons T. P. (ed) Biotechnology in the feed industry. Alltech Technical Publications, Nicholasville, Kentucky, 173-192, 1993. Cited by Iqbal et al., 2008.

Wallace, R.J., T.A. Wood, A. Rowe, J. Price, D.R. Yanez, S.P. Williams, and C.J. Newbold (2006). Encapsulated fumaric acid as a means of decreasing ruminal methane emissions. In: Greenhouse gases and animal agriculture: an update (ed. C.R. Soliva, J. Takahashi and M. Kreuzer), Elsevier International Congress Series 1293, Elsevier, Amsterdam, The Netherlands, 148-151, 2006. Cited by Martin et al., 2010.

Williams, Y.J., S. Popovski, S.M. Rea, L.C. Skillman, A.F. Toovey, K.S. Northwood, and A.D. Wright (2009). A vaccine against rumen methanogens can alter the composition of archaeal populations. Appl. Environ. Microbiol. 75: 1860-1866.

Wood, T.A., R.J. Wallace, A. Rowe, J. Price, D.R. Yanez-Ruiz, P. Murray, and C.J. Newbold (2009). Encapsulated fumaric acid as a feed ingredient to decrease ruminal methane emissions, Anim. Feed Sci. Technol. 152: 62-71.

Woodward, S.L., G.C. Waghorn, M.J. Ulyatt, and K.R. Lassey (2001). Early indications that feeding Lotus will reduce methane emission from ruminants. Proc. N.Z. Anim. Prod. 61: 23-26.

Woodward, S.L., G.C. Waghorn, K.R. Lassey, and P.G. Laboyrie (2002). Does feeding sulla (Hedysarum coronarium) reduce methane emission from dairy cows? Proc. N.Z. Soc. Anim. Sci. 62: 227-230.

Wright, A.D., P. Kennedy, C.J. O'Neill, A.F. Toovey, S. Popovski, S.M. Rea, C.L. Pimm, and L. Klein (2004). Reducing methane emissions in sheep by immunization against rumen methanogens. Vaccine. 22: 3976-3985.

Wright, A.D., C.H. Auckland, and D.H. Lynn (2007). Molecular diversity of methanogens in feedlot cattle from Ontario and Prince Edward Island, Canada. Appl. Environ. Microbiol. 73: 4206-4210.

Zijderveld, van S.M., B. Fonken, J. Dijkstra, J., W.J.J. Gerrits, H.B. Perdok, W. Fokkink, and J.R. Newbold (2011). Effects of a combination of feed additives on methane production, diet digestibility, and animal performance in lactating dairy cows. J. Dairy Sci. 94: 1445-1454.
COPYRIGHT 2018 Asianet-Pakistan
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2018 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Publication:Journal of Animal and Plant Sciences
Date:Apr 30, 2018

Terms of use | Privacy policy | Copyright © 2021 Farlex, Inc. | Feedback | For webmasters |