Nucleocapsid Protein as Early Diagnostic Marker for SARS.
Early laboratory diagnosis of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is one step in preventing recurrence of a global outbreak. The availability of the complete genomic sequence of SARS-CoV has facilitated the development of a variety of diagnostic tests for SARS (1). Reverse transcriptionpolymerase chain reaction (RT-PCR) has been used as a rapid diagnostic test in most of the research centers during the last epidemic (2-6). However, early diagnosis of SARS remains a problem for nonresearch laboratories with little experience in molecular testing. We have developed an antigen-capture enzyme-linked immunosorbent assay (ELISA) based on monoclonal antibodies against the nucleocapsid (N) protein of SARS-CoV (7), a predominant antigen produced in the infected cell-culture filtrate. High levels of circulating N protein can be detected in the serum samples of patients with SARS. We attempt to demonstrate the temporal profile of the N protein and antibodies in serum samples from a large cohort of patients with SARS during the acute and convalescent phases of the disease. Our findings suggest that detecting N protein in serum can be used as an early diagnostic marker for SARS.
During the 2003 SARS epidemic in Guangzhou, 420 serum specimens were collected from 317 patients 1-90 days after the onset of symptoms. The condition of all patients was diagnosed according to the World Health Organization criteria and confirmed by seroconversion or a fourfold increase in antibody titer against SARS-CoV by means of immunofluorescent testing. The N protein-capture ELISA was performed (7). Briefly, 100 [micro]L of serum was added to the wells of a microtiter plate coated with a mixture of three anti-N protein monoclonal antibodies, and the plates were incubated at 37[degrees]C for 60 min. After the plates were washed, 100 [micro]L of anti-N rabbit antiserum was added to the wells, and the plates were incubated at 37[degrees]C for 60 min. The wells were washed again and incubated for 1 h at 37[degrees]C with 100 [micro]L of peroxidase-conjugated goat anti-rabbit immunoglobulin (Ig)G. After the plates were washed, 100 [micro]L of tetramethylbenzidine solution was added to each well. The experiments involving the use of serum samples from patients with SARS were performed within the safety cabinet of a biosafety level 2 laboratory.
The results for the 420 serum specimens tested by the N protein--capture ELISA are shown in Figure 1. The N protein could be detected as early as day 1 and until day 18. In the 146 serum samples positive for N protein, the optical density (OD) value was highly variable from one sample to another on the same day. The sensitivity of detection was 94% (80 of 85 patients) with blood samples taken during the first 5 days and 78% (47 of 60 patients) for samples taken 6-10 days after onset of symptoms. The detection rate of N protein decreased to 27% on days 11-20 after onset of symptoms. Serum N protein was never detected beyond day 21. Using the same panel of the patient serum samples, we measured the N protein--specific IgG (SARS-CoV N-IgG) and SARS-CoV-specific IgG (SARS-CoV IgG) in serum samples by indirect ELISA, which progressively increased from day 7 onward (Figure 2). With the appearance of antibodies, the N protein detection rate decreased from day 10 after the onset of symptoms. However, from day 7 to day 18, a high level of N protein was still detectable in the serum samples from 11 patients with a mean of OD values of 1.65 when the SARS-CoV N-IgG had already increased to a level with a mean OD value of 1.18.
[FIGURES 1-2 OMITTED]
Serum samples from patients collected 4 years previously were used as negative controls. Patients thought to have cases of SARS on admission and later found to be uninfected by serologic testing, and healthcare workers who had close contact with SARS patients were tested for circulating N protein. Of the serum samples from non-SARS patients collected 4 years previously (n = 400), only one was weakly positive for N protein (OD = 0.288). We tested a total of 110 acute-phase serum samples from 105 patients, initially considered to have suspected SARS and later proven to be negative for SARS-CoV by serologic testing of convalescent-phase serum samples taken >28 days after onset of symptoms, and 315 serum samples from healthcare workers. All were negative for the N protein by capture ELISA. This finding resulted in a test specificity of 99.9% (1 of 825).
Our results suggest that N protein in the serum samples of SARS patients can be detected as early as day 1 after disease onset. Although the level of circulating N protein was highly variable from one person to another from day 1 to day 18, development of SARS-CoV N-IgG appears not to be affected by N protein during the acute phase of the infection, 7 days after the onset of symptoms. The positive detection rate of N protein in serum samples within the first 10 days of infection is higher than that detected by RT-PCR (8,9). Furthermore, the variation in the reported sensitivity and specificity of RT-PCR may be related to the lack of standardization of the assay and the specimen collection (6). Therefore, this viral antigen-capture ELISA may have greater sensitivity, specificity, and ease and reliability of in-use performance than the nucleic acid amplification assay. Further comparative studies with nucleic acid amplification tests should be undertaken at clinical laboratories serving acute-care hospitals where rapid SARS diagnosis is vital.
We thank Rongchang Chen and Yuanda Xu for providing the clinical data for analysis.
This work was supported by the special programs of SARS from the Ministry of Science and Technology of the People's Republic of China and the Research Project of Guangdong Province for SARS Prevention and Treatment.
(1.) Marra MA, Jones SJ, Astell CR, Holt RA, Brooks-Wilson A, Butterfield YS, et al. The genome sequence of the SARS-associated coronavirus. Science. 2003;300:1399-404.
(2.) Yu AC, Lau LT, Fung YW. Boosting the sensitivity of real-time polymerase-chain-reaction testing for SARS. N Engl J Med. 2004;350:1577-9.
(3.) Yam WC, Chan KH, Pooh LL, Guan Y, Yuen KY, Seto WH, et al. Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus. J Clin Microbiol. 2003;41:4521-4.
(4.) Jiang SS, Chen TC, Yang JY, Hsiung CA, Su IJ, Liu YL, et al. Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction. Clin Infect Dis. 2004;38:293-6.
(5.) Poon LL, Chan KH, Wong OK, Cheung TK, Ng I, Zheng B, et al. Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays. Clin Chem. 2004;50:67-72.
(6.) Emery SL, Erdman DD, Bowen MD, Newton BR, Winchell JM, Meyer RF, et al. Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus. Emerg Infect Dis. 2004;10:311-6.
(7.) Che XY, Qiu LW, Pan YX, Wen K, Hao W, Zhang LY, et al. Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome. J Clin Microbiol. 2004;42:2629-35.
(8.) Grant PR, Garson JA, Tedder RS, Chan PK, Tam JS, Sung JJ. Detection of SARS coronavirus in plasma by real-time RT-PCR. N Engl J Med. 2003;349:2468-9.
(9). Zhai J, Briese T, Dai E, Wang X, Pang X, Du Z, et al. Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003. Emerg Infect Dis. 2004;10:300-3.
The opinions expressed by authors contributing to this journal do not necessarily reflect the opinions of the Centers for Disease Control and Prevention or the institutions with which the authors are affiliated.
Xiao-Yan Che, * Wei Hao, * Yadi Wang, * Biao Di, ([dagger]) Kai Yin, * Yin-Chao Xu, * Chang-Sen Feng, * Zhuo-Yue Wan, ([double dagger]) Vincent C.C. Cheng, ([section]) and Kwok-Yung Yuen ([section])
* First Military Medical University, Guangzhou, People's Republic of China; ([dagger]) Center for Disease Control and Prevention of Guangzhou, Guangzhou, People's Republic of China; ([double dagger]) Center for Disease Control and Prevention of Guangdong Province, Guangzhou, People's Republic of China; and ([section]) The University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China
Address for correspondence: Xiao-Yan Che, Center of Laboratory, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, People's Republic of China; fax: 86-2061643592; email: firstname.lastname@example.org
Dr. Che is an immunologist and professor at First Military Medical University, Guangzhou, China. Her scientific interests are antibody technology and biotechnology.
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|Publication:||Emerging Infectious Diseases|
|Date:||Nov 1, 2004|
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