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Novel orthoreovirus from mink, China, 2011.

The family Reoviridae is a diverse group of viruses with double-stranded RNA genomes contained within icosahedral, nonenveloped, double-layered, protein capsids (1). Members of the genus Orthoreovirus contain 10 genome segments and have been isolated from a wide variety of reptiles, birds, and mammals (including humans) (1,2). The mammalian orthoreoviruses (MRVs) have 4 major serotypes: type 1 Lang, type 2 Jones,, type 3 Dearing, and type 4 Ndelle (3,4), which can be differentiated by neutralization and hemagglutination inhibition assays (5,6). It is well known that reovirus genomes are prone to various types of genome alterations, including intragenic rearrangement and reassortment under laboratory and natural conditions (7,8). Reassortment events, involving exchange of genome segments between 2 viruses, which could lead to increased virulence, are major driving forces for reovirus genome molecular diversity and evolution (9,10). In 1975, natural reovirus infection in mink was first described in Germany (11). In 1992, Liu et al. also reported the isolation of a reovirus from the feces of mink with diarrhea in China (12). However, to our knowledge, no genetic evidence of MRV strains isolated from mink has been reported.

We isolated a novel MRV strain (named MRV1HBA) from a mink with diarrhea in Hebei Province in northern China. To track virus evolution and look for evidence of genetic reassortment, we used PCR sequencing and phylogenetic analysis to compare genetic relatedness of MRV1HB-A and other orthoreoviruses.

The Study

In 2011, minks on a breeding farm in Raoyang County, in southeastern Hebei Province, became ill with an unidentified disease. The illness rate was almost 100% among farmed minks (Mustela vison), although the death rate was <5%, mainly in minks <3 months of age. Clinical signs included anorexia, emaciation, unkempt fur, and diarrhea. PCR excluded all classical endemic and emerging viruses, mink enteritis virus, canine distemper virus, Aleutian mink disease virus, and mink coronavirus as the causative agent. To identify the cause of the disease, we homogenized fecal samples from affected minks in phosphate-buffered saline and subsequently inoculated the homogenate into FK81, Vero, and BHK-21 cells. On day 7, a strong cytopathic effect was observed in FK81 cells, including rounded and detached cells; on day 8, a similar cytopathic effect was observed in Vero cells; and on day 10, the cytopathic effect was observed in BHK-21 cells. Electron microscopy of infected cells demonstrated icosahedral, nonenveloped, viral particles characteristic of MRVs (Figure 1). The mink reovirus was able to hemagglutinate type O human erythrocytes (1% vol/vol) but not chicken, mouse, goose, or rabbit erythrocytes (1% vol/vol), a finding characteristic of MRVs. Using MRV-specific reverse transcription PCR assays, we obtained products of the predicted size of 416 bp for the polymerase large (L)1 gene regions. After direct sequencing of the PCR products, a BLAST (www.ncbi.nlm.nih.gov/blast/ Blast.cgi) search showed the sequences to be authentic reovirus sequences, with closest similarity to those of the recently identified human MRV2tou05 strain, which had been isolated from 2 children with acute necrotizing encephalopathy in 2005 (10). These initial findings provide the genetic evidence that an enteric reovirus is shed in the diarrheal feces of mink, confirming a previous report suggesting MRV as an etiologic agent of acute viral enteritis in mink (12). We tested the pathogenicity of MRV1-HBA by orally infecting 3-month-old minks at a dose of 3 x [10.sup.5] 50% tissue culture infective dose. Mucoid diarrhea was seen on day 5 after infection. The clinical signs were similar to those of naturally infected mink.

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To further identify the virus and its phylogeny, we amplified and sequenced the MRV1-HB-A L1-L3, medium (M)1-M3, and small (S)1-S4 genes (GenBank accession nos. KC462149- KC462158). Primers used for all 10 segments are shown in the online Technical Appendix (wwwnc.cdc.gov/EID/article/19/12/13-0043-Techapp1.pdf). The nucleotide sequences obtained for each segment were compared with those of other orthoreoviruses by using the ClustalX1.83 (www.clustal.org) programs. Phylogenetic relationship was assessed by using 3 approaches: Bayesian phylogenetic trees, neighbor-joining, and split network. Topology was essentially the same for Bayesian trees, neighbor-joining, and split network (Figure 2; an expanded version of this figure is available online at wwwnc.cdc.gov/EID/article/19/12/13-0043-F2.htm). Sequence analysis showed that the 6 segments (L1-L3, M2, M3, and S3) of MRV1-HBA were closely related to those of the human MRV2tou05 strain and that the S1 and S2 segments were similar to those of other serotype 1 reoviruses that infect humans. The other 2 segments (M1 and S4) were closest to those of the SC-A strain, which was isolated from swine in 2006 in Sichuan, China (Table 1). The S1 segment of MRV1-HB-A is genetically similar to that of the human reovirus strains T1Neth/84 and T1Neth/85 (97% identity) isolated in the Netherlands in 1984 and 1985 (Figure 2). Nucleotide sequences of the prototype type 1 Lang and the MRV1-HB-A S1 genes shared 92% of positional identity, which provided sequence confirmation that this new isolate was a type 1 strain (Table 1). Otherwise, the S3 gene showed high identity with type 2 human reovirus Tou05 (98% identity) and porcine reovirus SHR-A (94% identity) strains (Figure 2). On the basis of these data, we conclude that the novel type 1 mink reovirus, designated MRV1-HB-A, might have originated from reassortment between human and swine strains.

Furthermore, we assessed the seroprevalence of mink reovirus antibodies in the human population and in 2 animal populations (mink and swine). Human serum samples were collected in Raoyao County People's Hospital (n = 181) of Hebei Province and Chaoyang District People's Hospital (n = 128) of Jilin Province. The human serum was donated by students, faculty members, and university workers, when they underwent annual health checkups at the 2 hospitals. From swine, 122 serum samples were collected in Hebei (n = 66) and Guangdong (n = 56) Provinces. From mink, 78 serum samples were collected in Hebei (n = 48) and Jilin (n = 30) Provinces. All samples were tested by hemagglutination inhibition and microneutralization assay as described (5,6) by using the MRV1-HB-A isolated strains as antigen. Samples with titers >16 were considered seropositive after 2 independent assays; lower titers were considered nonspecific reactions. For all groups, hemagglutination inhibition and microneutralization assays demonstrated similar trends, although the percentage of samples that were positive for MRV1-HB-A antibodies by microneutralization was slightly lower than by hemagglutination inhibition assay (Table 2). Because these antibodies were found in some, but not all, serum samples tested from each species and because the seropositive rate differed among regions, these antibodies probably reflect actual infection with reovirus MRV1-HB-A strain or with other strains that include a similar S1 gene.

Conclusions

Our study provides genomic evidence and molecular confirmation of a novel reovirus in mink. Although there is no direct evidence to prove the origin of MRV1-HBA, the close genetic relationship of MRV1-HB-A with strains from humans and swine indicated a high probability that MRV1-HB-A resulted from a reassortment of human and swine strains. In view of the lack of sequence data for the reovirus from mink in public databases, addition of the complete genome sequencing information for the mink reovirus will aid in the characterization of mammalian reovirus diversity and evolution. Although MRVs were assumed to cause rather mild respiratory or gastrointestinal diseases, recent findings indicate the occurrence of higher virulent MRV strains in man and other mammals. To be prepared for the potential emergence of more virulent variants, we should carefully monitor virus evolution in real time.

This research was funded by the China National "973" Program (approval no. 2011CB504700), the National Science Foundation of China (approval nos. 30972199, 31001070, 31372456), the Science and Technology Development Foundation of Jilin Province (approval nos. 20100146, 20130101092JC), and the Key Project of National Science Foundation of China (approval no. 30630049).

References

(1.) Attoui H, Mertens PPC, Becnel J, Belaganahalli S, Bergoin M, Brussaard CP, et al. Orthoreovirus, Reoviridae. In: Virus taxonomy. Classification and nomenclature of viruses: ninth report of the International Committee on the Taxonomy of Viruses. London: Elsevier Academic Press; 2011. p. 546-54.

(2.) Day JM. The diversity of the orthoreoviruses: molecular taxonomy and phylogenetic divides. Infect Genet Evol. 2009;9:390-400. http://dx.doi.Org/10.1016/j.meegid.2009.01.011

(3.) Kohl C, Lesnik R, Brinkmann A, Ebinger A, Radonic A, Nitsche A, et al. Isolation and characterization of three mammalian orthoreoviruses from European bats. PLoS ONE. 2012;7:e43106. http://dx.doi. org/10.1371/journal.pone.0043106

(4.) Attoui H, Biagini P, Stirling J, Mertens PP, Cantaloube JF, Meyer A, et al. Sequence characterization of Ndelle virus genome segments 1, 5, 7, 8, and 10: evidence for reassignment to the genus Orthoreovirus, family Reoviridae. Biochem Biophys Res Commun. 2001;287:583-8. http://dx.doi.org/10.1006/bbrc.2001.5612

(5.) Gravendyck M, Ammermann P, Marschang RE, Kaleta EF. Paramyxoviral and reoviral infections of iguanas on Honduran islands. J Wildl Dis. 1998;34:33-8.

(6.) Rosen L. Reoviruses in animals other than man. Ann N Y Acad Sci. 1962;101:461-5.

(7.) Duncan R. Extensive sequence divergence and phylogenetic relationships between the fusogenic and nonfusogenic orthoreoviruses: a species proposal. Virology. 1999;260:316-28. http://dx.doi. org/10.1006/viro. 1999.9832

(8.) Schriff LA, Nibert ML, Tyler KL. Orthoreoviruses and their replication. In: Knipe DM, Griffin DE, Lamb RA, Straus SE, Howley PM, et al., editors. Fields virology, 5th edition. Philadelphia: Lippincott Williams & Wilkins; 2007. p. 1853-915.

(9.) Chapell JD, Goral MI, Rodgers SE, dePamphilis CW, Dermody TS. Sequence diversity within the reovirus S2 gene: reovirus genes reassort in nature, and their termini are predicted to form a panhandle motif. J Virol. 1994;68:750-6.

(10.) OuattaraL A Barin F, Barthez MA, Bonnaud B, Roingeard P, Goudeau A, et al. Novel human reovirus isolated from children with acute necrotizing encephalopathy. Emerg Infect Dis. 2011;17:1436-44.

(11.) Kokles R. Isolation of reoviruses from mink [in German]. Arch Exp Veterinarmed. 1975;29:781-8.

(12.) Liu WQ, Han HM, Yang SH, Yao Q, Zhou XH, Zhang ZM, et al. Isolation, identification and pathogenicity of the enteric reovirus from mink [in Chinese]. Fur-Animal Breeding. 1992;14:1-3.

Address for correspondence: Rongliang Hu, Laboratory of Epidemiology, The Veterinary Research Institute, Academy of Military Medical Sciences, 666 Liuying West Rd, Changchun 130122, China; email: ronglianghu@hotmail.com

Author affiliation: Academy of Military Medical Sciences, Changchun, China

Dr Lian is an associate professor in the Laboratory of Epidemiology, Veterinary Research Institute, Academy of Military Medical Sciences, Changchun, China. His research interest is reovirus molecular epidemiology and vaccine development.
Table 1. Nucleotide identities for segments of novel mink
orthoreovirus MRV1HB-A, China *

                      Reovirus prototype strain, %
MRV1HB-A
RNA segment      T1L        T2J        T3D        T4N

L1               89.5       75.8       89.5       90.2
L2               86.5        73        77.2        NA
L3               84.3       77.5       84.5        NA
M1               94.9       70.2       94.4        NA
M2               84.7       76.6       89.7       89.1
M3               85.7       71.2       85.1        NA
S1              91.9#        58        40.6        42
S2              96.2#       77.2       85.4       85.6
S3               90.7       74.4       85.5        NA
S4               87.9       79.9       87.4       91.2

                Human reovirus
                    strain          Swine reovirus
                 MRV2tou05, %         strain, %
MRV1HB-A
RNA segment         Tou05           SC-A      SHR-A

L1                  97.4#           96.9       91.3
L2                  97.7#           95.9       84.5
L3                  97.2#            96        85.6
M1                    90           96.2#       94.9
M2                  96.4#           95.5       90.1
M3                  97.3#           96.6       85.5
S1                   58.6           40.9       73.3
S2                   84.7           85.9       92.7
S3                  97.8#            90        93.9
S4                   94.9          98.3#       78.1

* T1L, type1 Lang; T2J, type 2 Jones; T3D, type3 Dearing; T4N, type
4 Ndelle; L, large segment; NA, not available; M, medium segment; S,
small segment. Boldface indicates high sequence identity.

Note: High sequence identity are indicated with #.

Table 2. Responses of human and animal serum samples to novel mink
orthoreovirus MRV1-HB-A, China

                    Hemagglutination
                    inhibition, no.      Microneutralization,
Serum source,         positive/no.         no. positive/no.
province               tested (%)             tested (%)

Human
  Hebei              178/181 (98.3)         161/172 (93.6)
  Jilin              116/128 (90.6)         97/117 (82.9)
Swine
  Hebei                33/66 (50)            27/59 (45.8)
  Guangdong           50/56 (89.3)           44/52 (84.6)
Mink
  Hebei               48/48 (100)            46/46 (100)
  Jilin               17/30 (56.7)           15/28 (53.6)
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Article Details
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Title Annotation:DISPATCHES
Author:Lian, Hai; Liu, Ye; Zhang, Shoufeng; Zhang, Fei; Hu, Rongliang
Publication:Emerging Infectious Diseases
Article Type:Report
Geographic Code:9CHIN
Date:Dec 1, 2013
Words:2069
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