Notch signaling and T-helper cells in EAE/MS.
The evolutionary conserved Notch signaling pathway is a crucial player in cell fate decision from embryogenesis to adult life and plays a key role in a broad range of cellular processes including activation, proliferation, differentiation, and apoptosis. Notch signaling orchestrates normal cell and tissue development and has been implicated in the pathogenesis of some of the most challenging medical problems facing our society. In this review, we are going to focus on the influence of this pathway on autoimmune diseases.
The canonical Notch signaling cascade is initiated when a Notch receptor engages a Notch ligand expressed on a neighboring cell. This triggers a series of enzymatic reactions leading to the release of the Notch receptor intracellular domain, which translocates to the nucleus and forms an active transcription complex regulating target genes expression [1-3].
Multiple sclerosis (MS) is a chronic, often disabling autoimmune inflammatory demyelinating disease of the central nervous system (CNS) affecting mostly the young adult population. Unknown environmental factors still under investigation are thought to trigger MS in genetically predisposed individuals. T-helper (Th) cells, so called for their ability to coordinate and fine-tune the immune response, initiate an attack against "self" antigens expressed mainly on oligodendrocytes (OLs) leading to chronic inflammation . Notch signaling has been shown to regulate the development and function of both Th cells and OLs, with several groups reporting on the potential therapeutic implications of Notch pathway targeting in MS.
2. Notch Signaling
In 1914, John S. Dexter described a heritable "beaded" wing phenotype in the fruit fly Drosophila melanogaster. Twelve years later, Thomas H. Morgan published his work The Theory of the Gene in which he identified multiple mutant alleles resulting in this heritable "notched" wings phenotype. The gene was therefore appropriately called Notch. The Notch signaling pathway is now recognized as a cornerstone of cell-to-cell communication.
In humans, the classic Notch signaling pathway consists of four heterodimeric transmembrane receptors (Notch 1, 2, 3, and 4) and their ligands (Delta-like 1, 3, and 4 and Jagged 1 and 2) . The Notch receptor engagement by its ligand expressed on an adjacent cell is followed by two consecutive proteolytic reactions mediated by ADAM metalloproteases and the Presenilin family of [gamma]-secretases. These enzymatic reactions lead to the cleavage of the receptor in its transcellular domain region, releasing the Notch intracellular domain (NICD) which then translocates to the nucleus. Once in the nucleus, NICD forms a transcriptional complex with the recombination signal binding protein for immunoglobulin kappa J region (RBP-J[kappa]) and the coactivator mastermind-like (MAML) proteins, thus converting RBP-J[kappa] from a transcriptional repressor to a transcriptional activator. The NICD/RBP-J[kappa]/MAML complex then modulates the expression of their target genes [2, 3] Figure 1).
3. T-Helper Cell Differentiation
Three signals are required for efficient T cell differentiation. The first is in the form of antigen presented by an antigenpresenting cell (APC), such as a dendritic cell (DC). The second signal comes in the form of costimulatory receptors on T cells engaging their cognate ligands on APCs. Small signaling protein molecules, that is, cytokines, provide the third signal . Albeit an oversimplification, Notch signaling falls under the third signal category and fine-tunes the T cell response .
To date, numerous T-helper cell subsets have been defined mainly based on the expression of master transcriptional regulators and cytokine production profiles (Figure 2) . Antigen presentation in the presence of IL-12 induces the expression of T-bet and production of IFN-[gamma], therefore promoting naive T cell polarization into the Th1 phenotype. IL-4 induces GATA3 expression and IL-4 production and is necessary for Th2 cell polarization. IL-6 and TGF-[beta] induce RORyt expression and IL-17 production in Th17 cells. TGF-[beta] is necessary for foxp3 expression and regulatory T cell (Treg) differentiation. The IL-9 producing Th (Th9) cells require both IL-4 and TGF-[beta], which induce IRF4 and PU.1 expression, respectively .
While Th cell subsets are necessary for providing immunity against infectious pathogens, their aberrant response is to blame in several medical problems such as autoimmune diseases, allergies, and malignancies. Therefore, a Th cell type could be either "good" or "bad" depending on the immunological context. Studies in humans as well as in animal models of MS suggest that Th1 and Th17 cells are mostly pathogenic, while Th2 and Treg cells are anti-inflammatory. The role of Th9 cells in autoimmune diseases is still controversial as they might be a plastic, nonterminally differentiated phenotype .
4. Delta-Like Ligands and Th Subsets
Several in vitro studies support a role for Delta-like ligands (Dll) in promoting Th1 cell differentiation [9-11]. Briefly, APCs expressing Dll promote Th1 while suppressing Th2 cell differentiation. Concurrently, exogenous stimuli that would enhance APCs polarizing potential of Th1 cells also increase the APCs expression of Dll . RBP-J[kappa] and NICD were reported to bind to the Tbx21 and Ifng promoters, respectively, two hallmarks of Th1 cells [10,11].
With regard to Th17 cells, Mukerjee et al. show that under Th17 polarizing conditions rDll4 treatment significantly enhances IL-17 production while [gamma]-secretase inhibitor (GSI) mediated inhibition of Notch signaling abrogates it. Furthermore, RBP-Jk was found to bind to the Il17 promoter and this was reduced in the presence of GSI .
Bassil et al. show that Dll4 mediated signaling inhibits TGF-[beta]-induced Treg development as well as Janus kinase 3-induced STAT5 phosphorylation, a transcription factor known to play a key role in Foxp3 expression and maintenance . The role of Dll4 in Treg development was further confirmed by Billiard et al. by showing that anti-Dll4 Ab treatment converts early T cell progenitors to immature tolerogenic DCs that promote Treg-cell expansion . Adding another dimension to the picture, Hue et al. demonstrate that pretreatment with Notch ligands Dll4 and Jagged1 sensitizes [CD4.sup.+] [CD25.sup.-] effector T cells to Treg-cell mediated suppression through increased TGF-[beta]RII expression and Smad3 phosphorylation .
5. Jagged Ligands and Th Subsets
What applies to the Dll and Th1/Th2 cells is almost opposite to the findings seen with the Jagged ligands. APCs expressing Jagged ligands promote Th2 cells while suppressing Th1 cell differentiation. Concurrently, pathogens that enhance APCs polarizing potential for Th2 cells also increase the APCs expression of Jagged ligands . Furthermore, Notch and RBP-J[kappa] were found to bind the Gata3 promoter and the HS5 site of the IL4 enhancer, both critical genes in Th2 cell differentiation [9,16,17].
Jagged ligands are thought to enhance the development and function of regulatory T cells. In a human in vitro study, Vigouroux et al. report on the induction of an antigen specific IL-10 producing regulatory T cell population (Tr1) following stimulation by Jagged1 transduced B cells . Kared et al. show that a population of hematopoietic progenitor cells (HPCs) highly expressing Jagged2 ligand activated Notch3 signaling in Treg cells enhancing their expansion and suppressive function. This signaling mechanism required cell-tocell interaction and was inhibited by GSI .
Asano et al. have demonstrated that Treg suppressor cells express Jagged1 while the responder cells ([CD4.sup.+][CD25.sup.-]) express Notch1. Anti-Notch1 and to a lesser extent antiJagged1 Abs inhibited the suppressive function of Treg cells. Furthermore, they show that Jagged1-mediated Notch1 activation enhances TGF-[beta]-induced Smad3 transcription and translocation to the nucleus, a key component of TGF-[beta] mediated signaling .
With regard to Th9 cells, Elyaman et al. have found Notch1 and Notch2 conditional ablation to significantly reduce IL-9 production. In fact, Jagged2 mediated Notch signaling promotes RBP-J[kappa]/NICD1/Smad3 transcriptional complex formation and binding and transactivation of the Il9 promoter .
6. Notch Intracellular Domain and Noncanonical Signaling in Th Subsets
In addition to the data that has been generated involving the Delta-like and Jagged ligands, a plurality of data has been generated without regard to ligand to show Notch involvement in T-helper subset differentiation, and more work will need to be done to fully elucidate the specific ligand pathway.
RBP-J[kappa] and NICD have been shown to bind the Gata3 promoter, without specific ligand activation [16,17]. Similar results have been shown for the Tbx21 and Ifng promoters as well [8,9]. Thusthe specificligandpathway of many aspectsof Notch signaling remains to be determined despite consistent results showing involvement in Th development.
Another topic of active research is the role of noncanonical Notch signaling in Th differentiation. Perumalsamy et al. found that NICD in the plasma membrane, rather than the nucleus, was associated with improved survival of Tregs . Additionally Auderset et al. showed Notch signaling independent of RBP-J[kappa] to be important for Th1 development during parasitic infections . The increasing body of evidence points to a significant role for noncanonical Notch signaling in the differentiation and proliferation of Th subsets (see Table 1), and this will likely be an active area of research in the future.
7. Notch and Oligodendrocytes
Oligodendrocyte (OL) projections provide neurons with a protective and insulating myelin sheath, which optimizes nerve conduction speeds. The autoimmune response targeting this myelin sheath results in slowing nerve conduction velocities and is responsible for the neurological deficits in MS. Therefore, immunoregulatory approaches targeting oligodendrocyte progenitor cell (OPC) proliferation and differentiation would be invaluable. It is worth noting that several groups have demonstrated that the timing of Notch signaling differentially regulates OPC development, with Dll1- and Jagged1-mediated signaling inhibiting OPC maturation while enhancing their expansion [24-26].
8. Notch and Animal Models of MS
Experimental autoimmune encephalomyelitis (EAE), the most widely used model for MS [27,28], is induced by active immunization of mice with myelin antigens emulsified in adjuvant . Alternatively, EAE can be induced by passive transfer of activated myelin-specific cellular clones or cell lines . Theiler's murine encephalomyelitis virus-(TMEV-) induced demyelinating disease (TMEV-IDD), another popular model for MS, is induced by intracerebral injection with TMEV resulting in CNS inflammation .
Minter et al. nonspecifically inhibited Notch signaling by oral or intraperitoneal administration of GSI in the PLP/SJL EAE model. This resulted in a significant decrease in clinical disease and Th1 associated cytokines reduction . Keerthivasan et al. followed up on this work by showing that Notch plays a role in Th17 differentiation and GSI in the PLP/SJL EAE model reduces IL-17 production .
Jurynczyk et al. provided compelling evidence that Notch3 may play a significant role in EAE when they showed that, by using GSI against specific Notch3 and not Notch1, there is a significant decrease in clinical disease score as well as Th1 and Th17 cytokines using the PLP/SJL EAE model .
Among all Notch ligands, the role of Dll4 in animal models of MS has been the most studied role. In 2010, Takeichi et al. showed that Dll4 expression is significantly upregulated on DCs in the TMEV-IDD model. Dll4 blockade significantly ameliorated the clinical course of the disease, which was attributed to a decrease in mononuclear cell infiltration of the target tissues and reduction in IFN-[gamma] and IL-17 production .
In 2011, in concordance with the TMEV-IDD study, Reynolds et al. described an increase in Dll4 expression on APCs in the PLP/SJL EAE model, with Dll4 blockade alleviating clinical disease and decreasing IFN-[gamma] and IL-17 producing [CD4.sup.+] T cells frequency and leukocyte infiltration of the CNS, while having no effect on the Foxp3 mRNA expression levels. Reynolds et al. attribute the effects observed with Dll4 blockade to a downregulation of the chemokine receptors CCR2 and CCR6 expression on [CD4.sup.+] T cells, leading to their differential migration and accumulation in the CNS . Also in 2011 and in agreement with the previous studies, Bassil et al. showed that Dll4 blockade in the MOG/B6 EAE model alleviates the clinical EAE severity and shifts the immune balance from a Th1/Th17 mediated response toward a Th2/Treg mediated response. In this study, the effects were mainly attributed to the role Dll4 plays in regulating Treg development, with Treg depletion prior to EAE induction abrogating the anti-Dll4 mAb protective effect .
Dll1 contribution to the EAE model has been described by Elyaman et al. in 2007, showing DC upregulation of Dll1 expression during the induction phase of the disease. Dll1 blockade reduced the disease severity and [CD4.sup.+][IFN-[gamma].sup.+] cell frequency, while Dll1 ligation had the opposite effect. Modulation of the Dll1 mediated signaling had no effect on [CD4.sup.+][Foxp3.sup.+] cell frequencies . Tsugane et al. reported on Dll1 blockade in the TMEV-IDD model in 2012. A decrease in IFN-[gamma], IL-4, and IL-10 producing [CD4.sup.+] T cells and an increase in IL-17 producing [[CD4.sup.+].sup.T] cells were observed in the spinal cords of treated mice. This resulted in a significant suppression of the disease both clinically and histologically .
The role of the Jagged ligands in animal models of MS has not been studied as much as their Dll counterparts. Our group has shown that the administration of anti-Jagged1 mAb exacerbated EAE clinical disease and was associated with a decrease in IL-10-producing [CD4.sup.+] T cells in the CNS. In contrast, the administration of Jagged1-Fc protected the mice from disease and increased the frequency of IL-10producing [CD4.sup.+] T cells 35]. Using a human Jagged1 agonist peptide, Palacios et al. have also concluded that Jagged1 signaling ameliorates EAE course, which was associated with an increase in [CD25.sup.+][Foxp3.sup.+] T cell frequency . In a recent study, Elyaman et al. reported that the timing of Jagged2 mediated signaling differentially regulates EAE. In that report, we show that Notch signaling is required for optimal IL-9 production. Jagged2 signaling molecule administration before antigen immunization promotes IL9-mediated Treg-cell expansion and suppresses EAE, while Jagged2 signaling molecule administration concurrent with immunization worsens EAE, with IL-9 favoring Th17 cell expansion in this inflammatory milieu . The role of Notch signaling in animal models of MS is summarized in Table 2.
Notch signaling has been investigated in other models of immune mediated diseases and the data complements the findings in the EAE system. Not surprisingly, the effect on the clinical disease was largely dependent on the immunological context. The data is summarized in Table 3.
9. Notch and MS
Despite the overwhelming evidence supporting the role of Notch signaling in Th cell development and in regulating the outcome in animal models of MS, studies in the human system remain scarce and mostly point to Jagged1 or were ligand independent.
Zhang et al. studied chronic active MS lesions and concluded that the expression of Jagged1 in remyelinated MS lesions is nonsignificant. On the other hand, in active MS lesions lacking remyelination, Jagged1 is highly expressed by hypertrophic astrocytes, with Notch1 being preferentially expressed in nondifferentiated OLs . In a study of chronic silent MS lesions, Nakahara et al. observed a high level of activation of Notch1 through the noncanonical Notch signaling pathway, while the classic Notch signaling pathway is inhibited .
An analysis of gene networks regulating T cell activation in MS patients by Palacios et al. has concluded that Jaggedl is consistently modified in the disease state making it a potential therapeutic target in MS . However, the strongest inculpating evidence emerged in 2006 when a meta-analysis of the Genetic Analysis of Multiple Sclerosis in EuropeanS (GAMES) project involving 13,896 individuals identified Jaggedl as a susceptibility gene for MS .
These observations taken together with the data from in vitro studies further highlight the key role of the Notch signaling pathway in regulating the immune balance in MS.
10. Concluding Remarks
The scientific community has provided overwhelming evidence implicating the Notch signaling pathway in the pathogenesis of autoimmune diseases including MS. Notch-mediated signaling emerges as a key regulator of the development of Th cell subsets promoting autoimmunity, as well as other Th subsets playing an anti-inflammatory role [4,10,13, 21, 35]. This dichotomyhas also been demonstrated in OPCs where the nature and timing of Notch signaling could either enhance or inhibit OPC maturation and expansion [25, 26]. Therefore, Notch signaling regulates the development and function of pathogenic cells as well as cells with regenerative and anti-inflammatory properties. This makes Notch signaling targeted immunotherapy extremely promising yet problematic for the same reason. To complicate the picture, while it seems likely that Th subsets are a valid target for Notch immunotherapy, APCs and other myeloid cells clearly play a role in EAE and should not be excluded as potential cell-specific targets.
The obvious challenges arise from the difficulties in delivering the right immunomodulatory signal to the right target cell at the right time. To further complicate the picture, Notch receptors and ligands are ubiquitously expressed making the nonselective approach less than ideal. We believe that the current literature supports and encourages a Notch signaling targeted immunotherapy even in a noncell-specific targeting system through the use of signaling pathway inhibitors such as GSI or the use of mAbs and signaling molecules. However, harnessing the immense therapeutic potential of the Notch signaling pathway modulation lies in taking advantage of future advances and breakthroughs in cell-specific targeted drug delivery systems.
 R. J. Fleming, "Structural conservation of Notch receptors and ligands," Seminars in Cell and Developmental Biology, vol. 9, no. 6, pp. 599-607, 1998.
 C. J. Fryer, E. Lamar, I. Turbachova, C. Kintner, and K. A. Jones, "Mastermind mediates chromatin-specific transcription and turnover of the notch enhancer complex," Genes and Development, vol. 16, no. 11, pp. 1397-1411, 2002.
 S. J. Bray, "Notch signalling: a simple pathway becomes complex," Nature Reviews Molecular Cell Biology, vol. 7, no. 9, pp. 678-689, 2006.
 M. Comabella and S. J. Khoury, "Immunopathogenesis of multiple sclerosis," Clinical Immunology, vol. 142, no. 1, pp. 2-8, 2012.
 M. L. Kapsenberg, "Dendritic-cell control of pathogen-driven T-cell polarization," Nature Reviews Immunology, vol. 3, no. 12, pp. 984-993, 2003.
 H. Yamane and W. E. Paul, "Early signaling events that underlie fate decisions of naive CD4(+) T cells toward distinct T-helper cell subsets," Immunological Reviews, vol. 252, no. 1, pp. 12-23, 2013.
 J. Zhu, H. Yamane, and W. E. Paul, "Differentiation of effector [CD4.sup.+] T cell populations," Annual Review of Immunology, vol. 28, pp. 445-489, 2010.
 F. Petermann and T. Korn, "Cytokines and effector T cell subsets causing autoimmune CNS disease," FEBS Letters, vol. 585, no. 23, pp. 3747-3757, 2011.
 D. Amsen, J. M. Blander, G. R. Lee, K. Tanigaki, T. Honjo, and R. A. Flavell, "Instruction of distinct CD4 T helper cell fates by different notch ligands on antigen-presenting cells," Cell, vol. 117, no. 4, pp. 515-526, 2004.
 L. M. Minter, D. M. Turley, P. Das et al., "Inhibitors of [gamma]-secretase block in vivo and in vitro T helper type 1 polarization by preventing Notch upregulation of Tbx21," Nature Immunology, vol. 6, no. 7, pp. 680-688, 2005.
 H. M. Shin, L. M. Minter, H. C. Ok et al., "Notch1 augments NFkB activity by facilitating its nuclear retention," EMBO Journal, vol. 25, no. 1, pp. 129-138, 2006.
 S. Mukherjee, M. A. Schaller, R. Neupane, S. L. Kunkel, and N. W. Lukacs, "Regulation of T cell activation by notch ligand, DLL4, promotes IL-17 production and Rorc activation," Journal of Immunology, vol. 182, no. 12, pp. 7381-7388, 2009.
 R. Bassil, B. Zhu, Y. Lahoud et al., "Notch ligand delta-like 4 blockade alleviates experimental autoimmune encephalomyelitis by promoting regulatory T cell development," Journal of Immunology, vol. 187, no. 5, pp. 2322-2328, 2011.
 F. Billiard, C. Lobry, G. Darrasse-Jeze et al., "Dll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice," Journal of Experimental Medicine, vol. 209, no. 5, pp. 1011-1028, 2012.
 S. Hue, H. Kared, Y. Mehwish, S. Mouhamad, M. Balbo, and Y. Levy, "Notch activation on effector T cells increases their sensitivity to Treg cell-mediated suppression through upregulation of TGF-betaRII expression," European Journal of Immunology, vol. 42, no. 7, pp. 1796-1803, 2012.
 D. Amsen, A. Antov, D. Jankovic et al., "Direct regulation of Gata3 expression determines the T helper differentiation potential of Notch," Immunity, vol. 27, no. 1, pp. 89-99, 2007
 T. C. Fang, Y. Yashiro-Ohtani, C. Del Bianco, D. M. Knoblock, S. C. Blacklow, and W. S. Pear, "Notch directly regulates Gata3 expression during T helper 2 cell differentiation," Immunity, vol. 27, no. 1, pp. 100-110, 2007
 S. Vigouroux, E. Yvon, H. J. Wagner et al., "Induction of antigenspecific regulatory T cells following overexpression of a Notch ligand by human B lymphocytes," Journal of Virology, vol. 77, no. 20, pp. 10872-10880, 2003.
 H. Kared, H. Adle-Biassette, E. Fols et al., "Jagged2-expressing hematopoietic progenitors promote regulatory T cell expansion in the periphery through notch signaling," Immunity, vol. 25, no. 5, pp. 823-834, 2006.
 N. Asano, T. Watanabe, A. Kitani, I. J. Fuss, and W. Strober, "Notch1 signaling and regulatory T cell function," Journal of Immunology, vol. 180, no. 5, pp. 2796-2804, 2008.
 W. Elyaman, R. Bassil, E. M. Bradshaw et al., "Notch receptors and Smad3 signaling cooperate in the induction of interleukin9-producingT cells," Immunity, vol. 36,no. 4,pp. 623-634,2012.
 L. R. Perumalsamy, N. Marcel, S. Kulkarni, F. Radtke, and Sarin, "Distinct spatial and molecular features of notch pathway assembly in regulatory T cells," Science Signaling, vol. 5, no. 234, article ra53, 2012.
 F. Auderset, S. Schuster, M. Coutaz et al., "Redundant Notch1 and Notch2 signaling is necessary for IFNgamma secretion by T helper 1 cells during infection with Leishmania major," PLoS Pathogens, vol. 8, no. 3, Article ID e1002560, 2012.
 S. Wang, A. D. Sdrulla, G. DiSibio et al., "Notch receptor activation inhibits oligodendrocyte differentiation," Neuron, vol. 21, no. 1, pp. 63-75, 1998.
 S. Genoud, C. Lappe-Siefke, S. Goebbels et al., "Notch1 control of oligodendrocyte differentiation in the spinal cord," Journal of Cell Biology, vol. 158, no. 4, pp. 709-718, 2002.
 Y. Zhang, A. T. Argaw, B. T. Gurfein et al., "Notch1 signaling plays a role in regulating precursor differentiation during CNS remyelination," Proceedings of the National Academy of Sciences of the United States of America, vol. 106, no. 45, pp. 19162-19167, 2009.
 A. G. Baxter, "The origin and application of experimental autoimmune encephalomyelitis," Nature Reviews Immunology, vol. 7, no. 11, pp. 904-912, 2007
 R. M. Ransohoff, "Animal models of multiple sclerosis: the good, the bad and the bottom line," Nature Neuroscience, vol. 15, no. 8, pp. 1074-1077, 2012.
 I. M. Stromnes and J. M. Goverman, "Active induction of experimental allergic encephalomyelitis," Nature Protocols, vol. 1, no. 4, pp. 1810-1819, 2006.
 I. M. Stromnes and J. M. Goverman, "Passive induction of experimental allergic encephalomyelitis," Nature Protocols, vol. 1, no. 4, pp. 1952-1960, 2006.
 S. Keerthivasan, R. Suleiman, R. Lawlor et al., "Notch signaling regulates mouse and human Th17 differentiation," Journal of Immunology, vol. 187, no. 2, pp. 692-701, 2011.
 M. Jurynczyk, A. Jurewicz, C. S. Raine, and K. Selmaj, "Notch3 inhibition in myelin-reactive T cells down-regulates protein kinase CO and attenuates experimental autoimmune encephalomyelitis," Journal of Immunology, vol. 180, no. 4, pp. 2634-2640, 2008.
 D. Skokos and M. C. Nussenzweig, "CD8- DCs induce IL12-independent Th1 differentiation through Delta 4 Notch-like ligand in response to bacterial LPS," Journal of Experimental Medicine, vol. 204, no. 7, pp. 1525-1531, 2007
 J. Sun, C. J. Krawczyk, and E. J. Pearce, "Suppression of Th2 cell development by Notch ligands Delta1 and Delta4," Journal of Immunology, vol. 180, no. 3, pp. 1655-1661, 2008.
 W. Elyaman, E. M. Bradshaw, Y. Wanget al., "Jagged1 and delta1 differentially regulate the outcome of experimental autoimmune encephalomyelitis," Journal of Immunology, vol. 179, no. 9, pp. 5990-5998, 2007.
 S. Tsugane, S. Takizawa, T. Kaneyama et al., "Therapeutic effects of anti-Delta1 mAb on Theiler's murine encephalomyelitis virus-induced demyelinating disease," Journal of Neuroimmunology, vol. 252, no. 1-2, pp. 66-74, 2012.
 N. Takeichi, S. Yanagisawa, T. Kaneyama et al., "Ameliorating effects of anti-Dll4 mAb on Theiler's murine encephalomyelitis virus-induced demyelinating disease," International Immunology, vol. 22, no. 9, pp. 729-738, 2010.
 N. D. Reynolds, N. W. Lukacs, N. Long, andW. J. Karpus, "Deltalike ligand 4 regulates central nervous system T cell accumulation during experimental autoimmune encephalomyelitis," Journal of Immunology, vol. 187, no. 5, pp. 2803-2813, 2011.
 R. Palacios, J. Goni, I. Martinez-Forero et al., "A network analysis of the human T-cell activation gene network identifies Jagged1 as a therapeutic target for autoimmune diseases," PLoS ONE, vol. 2, no. 11, Article ID e1222, 2007
 A. Fukushima, T. Sumi, W. Ishida et al., "Notch ligand Deltalike4 inhibits the development of murine experimental allergic conjunctivitis," Immunology Letters, vol. 121, no. 2, pp. 140-147, 2008.
 M. T. Huang, Y. S. Dai, Y. B. Chou, Y. H. Juan, C. C. Wang, and B. L. Chiang, "Regulatory T cells negatively regulate neovasculature of airway remodeling via DLL4-notch signaling," Journal of Immunology, vol. 183, no. 7, pp. 4745-4754, 2009.
 S. Jang, M. Schaller, A. A. Berlin, and N. W. Lukacs, "Notch ligand delta-like 4 regulates development and pathogenesis of allergic airway responses by modulating IL-2 production and Th2 immunity," Journal of Immunology, vol. 185, no. 10, pp. 5835-5844, 2010.
 W. Ishida, K. Fukuda, S. Sakamoto et al., "Regulation of experimental autoimmune uveoretinitis by anti-delta-like ligand 4 monoclonal antibody," Investigative Ophthalmology & Visual Science, vol. 52, no. 11, pp. 8224-8230, 2011.
 K. Mochizuki, F. Xie, S. He et al., "Delta-like ligand 4 identifies a previously uncharacterized population of inflammatory dendritic cells that plays important roles in eliciting allogeneic T cell responses in mice," Journal of Immunology, vol. 190, no. 7, pp. 3772-3782, 2013.
 L. V. Riella, T. Ueno, I. Batal et al., "Blockade of notch ligand delta1 promotes allograft survival by inhibiting alloreactive Th1 cells and cytotoxic T cell generation," Journal of Immunology, vol. 187, no. 9, pp. 4629-4638, 2011.
 M. Okamoto, H. Matsuda, A. Joetham et al., "Jagged1 on dendritic cells and notch on [CD4.sup.+] T cells initiate lung allergic responsiveness by inducing IL-4 production," Journal of Immunology, vol. 183, no. 5, pp. 2995-3003, 2009.
 L. V. Riella, J. Yang, S. Chock et al., "Jagged2-signalingpromotes IL-6-dependent transplant rejection," European Journal of Immunology, vol. 43, no. 6, pp. 1449-1458, 2013.
 J. Nakahara, K. Kanekura, M. Nawa, S. Aiso, and N. Suzuki, "Abnormal expression of TIP30 and arrested nucleocytoplasmic transport within oligodendrocyte precursor cells in multiple sclerosis," Journal of Clinical Investigation, vol. 119, no. 1, pp. 169-181, 2009.
 M. Ban, D. Booth, R. Heard et al., "Linkage disequilibrium screening for multiple sclerosis implicates JAG1 and POU2AF1 as susceptibility genes in Europeans," Journal of Neuroimmunology, vol. 179, no. 1-2, pp. 108-116, 2006.
Ribal Bassil, William Orent, and Wassim Elyaman
Center for Neurologic Diseases, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA
Correspondence should be addressed to Wassim Elyaman; firstname.lastname@example.org
Received 19 May 2013; Accepted 25 September 2013
Academic Editor: Carlos Barcia
TABLE 1: Notch and Th subsets. Ligand/pathway J Dll BMDC LPS stimulation Dll Dll1 expressing APC/[CD4.sup.+] T cells coculture Dll [CD8.sup.-] DCs LPS stimulation Dll Dll4-mFc [CD4.sup.+] T cell treatment Dll DCs TLR2/TLR9 ligation Dll [CD4.sup.+] T cell recDll4 treatment Dll [CD4.sup.+] T cell recDll4 treatment Dll [CD4.sup.+] [CD25.sup.-] cells Dll4 and Jagged1 pretreatment Jagged Jagged1 transduction of human APCs Jagged HPCs expressing Jagged2 Jagged Notch1 or Jagged1 blockade Jagged BMDC LPS stimulation Jagged Jagged1 expressing APCs/[CD4.sup.+] T cells coculture NICD NICD forced expression in [CD4.sup.+] T cells NICD RBP-Jx/NICD1/Smad3 forced expression in [CD4.sup.+] T cells NICD Cell line transduction NICD Splenocytes aCD3/aCD28 stimulation NICD NICD forced expression in [CD4.sup.+] T cells NICD Notch1 blockade in Th17 cells NICD Cell line transfection Noncanonical In vivo notch ablation in [CD4.sup.+] cells Noncanonical Mutant NICD in Notch1 KO Tregs Ligand/pathway Results Dll [up arrow] Dll4 mRNA Dll [up arrow] IFN-g Dll [up arrow] Dll4 expression Dll [up arrow] IFN-g Dll [up arrow] DCs Dll expression, [up arrow] T-bet, [up arrow] IFN-g, and [up arrow] IL-4 by [CD4.sup.+] T cel Dll [up arrow] RORc activation, [up arrow] IL-17 Dll [up arrow] phospho-Jak3, [up arrow] phospho-Stat5, and [up arrow] Foxp3 Dll [up arrow] TGF-[beta]RII and phospho-Smad3 Jagged Induction of IL-10 producing Tr1 cells Jagged [up arrow] Treg expansion and function Jagged [up arrow] Treg function Jagged [up arrow] Jagged1 mRNA Jagged [up arrow] IL-4, [up arrow] IL-5 NICD NICD regulates IL4 transcription NICD RBP-J[kappa]/NICD1/Smad3 complex binds and transactivates Il9 promoter NICD RBP-J[kappa] binds Tbx21 promoter NICD NICD binds Ifng promoter NICD NICD binds the Gata3 promoter NICD [up arrow] Th17 associated cytokines NICD RBP-Jk binds the Gata3 promoter Noncanonical Notch1 and Notch2 redundantly essential for Th1 development Noncanonical NICD targeting plasma membrane improves Treg survival Ligand/pathway References Dll Amsen et al., 2004  Dll Dll Skokos and Nussenzweig, 2007  Dll Dll Sun et al., 2008  Dll Mukherjee et al., 2009  Dll Bassil et al., 2011  Dll Hue et al., 2012  Jagged Vigouroux et al., 2003  Jagged Kared et al., 2006  Jagged Asano et al., 2008  Jagged Amsen et al., 2004  Jagged NICD NICD Elyaman et al., 2012  NICD Minter et al., 2005  NICD Shin et al., 2006  NICD Fang etal., 2007  NICD Keerthivasan et al., 2011  NICD Amsen et al., 2007  Noncanonical Auderset et al., 2012  Noncanonical Perumalsamy et al., 2012  TABLE 2: Notch and animal models of MS. MS animal Method model EAE (PLP/SJL) GSI EAE (PLP/SJL) Anti-Notch3 EAE (PLP/SJL) GSI EAE (MOG/B6) Anti-Dll1 TMEV-IDD Anti-Dll1 TMEV-IDD Anti-Dll4 EAE (PLP/SJL) Anti-Dll4 EAE (MOG/B6) Anti-Dll4 EAE (MOG/B6) Anti-Jagged1 EAE (MOG/B6) Jagged1 peptide EAE (MOG/B6) Anti-Jagged2 signaling molecules prior to immunization Anti-Jagged2 signaling molecules at time of immunization MS animal Results model EAE (PLP/SJL) [down arrow] Disease, [down arrow] Th1 EAE (PLP/SJL) [down arrow] Disease, [down arrow] Th1, and [down arrow] Th17 EAE (PLP/SJL) [down arrow] Disease, [down arrow] Th17 EAE (MOG/B6) [down arrow] Disease, [down arrow] Th1 TMEV-IDD [down arrow] Disease, [down arrow] IFN-[gamma], and [down arrow] IL-4 TMEV-IDD [down arrow] Disease, [down arrow] IFN-[gamma], and [down arrow] IL-17 EAE (PLP/SJL) [down arrow] Disease, [down arrow] Th1, and [down arrow] Th17 EAE (MOG/B6) [down arrow] Disease, [down arrow] Th1, [down arrow] Th17, [down arrow] Th2, and [up arrow] Treg EAE (MOG/B6) [down arrow] Disease, [down arrow] IL-10 EAE (MOG/B6) [down arrow] Disease, [down arrow] IFN-[gamma], and [up arrow] IL-4 EAE (MOG/B6) [down arrow] Disease, [up arrow] Treg [up arrow] Disease, [up arrow] IL-17 MS animal References model EAE (PLP/SJL) Minter et al., 2005  EAE (PLP/SJL) Jurynczyk et al., 2008  EAE (PLP/SJL) Keerthivasan et al., 2011  EAE (MOG/B6) Elyaman et al., 2007  TMEV-IDD Tsugane et al., 2012  TMEV-IDD Takeichi et al., 2010  EAE (PLP/SJL) Reynolds et al., 2011  EAE (MOG/B6) Bassil et al., 2011  EAE (MOG/B6) Elyaman et al., 2007  EAE (MOG/B6) Palacios et al., 2007  EAE (MOG/B6) Elyaman et al., 2012  TABLE 3: Notch and animal models of immune mediated diseases. Animal model Method Allergic conjunctivitis Anti-Dll4 Allergic asthma Anti-Dll4 Allergic airway response Anti-Dll4 Autoimmune uveoretinitis Anti-Dll4 T1D Anti-Dll4 Graft versus host disease Anti-Dll4 Allogeneic cardiac Anti-Dll1 transplant Airway hyperresponsiveness Jagged1-Fc Murine cardiac transplant Anti-Jagged2 signaling Ab Animal model Results Allergic conjunctivitis [up arrow] Disease, [up arrow] Th2 Allergic asthma [up arrow] Disease, [down arrow] Treg function Allergic airway response [up arrow] Disease, [up arrow] Th2 Autoimmune uveoretinitis [up arrow] Disease, [down arrow] Th17 T1D [down arrow] Disease, [up arrow] Treg Graft versus host disease [up arrow] Survival, [down arrow] Th1, and [down arrow] Th17 Allogeneic cardiac [up arrow] Survival, [down arrow] Th1, transplant and [down arrow] cytotoxic T cell Airway hyperresponsiveness [up arrow] Disease, [up arrow] Th2 Murine cardiac transplant [down arrow] Survival, [up arrow] IL-2, and [up arrow] IL-6 Animal model References Allergic conjunctivitis Fukushima et al., 2008  Allergic asthma Huang et al., 2009  Allergic airway response Jang et al., 2010  Autoimmune uveoretinitis Ishida et al., 2011  T1D Billiard et al., 2012  Graft versus host disease Mochizuki et al., 2013  Allogeneic cardiac Riella et al., 2011  transplant Airway hyperresponsiveness Okamoto et al., 2009  Murine cardiac transplant Riella et al., 2013 
|Printer friendly Cite/link Email Feedback|
|Title Annotation:||Encephalomyelitis; Multiple sclerosis|
|Author:||Bassil, Ribal; Orent, William; Elyaman, Wassim|
|Publication:||Journal of Immunology Research|
|Date:||Jan 1, 2013|
|Previous Article:||In utero hepatocellular transplantation in rats.|
|Next Article:||Development of a recombinant cell-based indirect immunofluorescence assay for the determination of autoantibodies against soluble liver antigen in...|