Printer Friendly

New Delhi Metallo-[beta]-Lactamase-1 in Carbapenem resistant Salmonella Strain, China.

To the Editor: Carbapenem resistance in Enterobacteriaceae can occur through the production of carbapenem-hydrolyzing enzymes such as New Delhi metallo-[beta-lactamase-1 (NDM-1) (1). In recent years, plasmid-mediated NDM-1 has spread rapidly worldwide and into multiple Enterobacteriaceae species, such as Klebsiella pneumoniae and Escherichia coli (2).

NDM-1 has been reported in 2 strains of Salmonella spp., which were isolated from feces and urine specimens during screening for multidrug-resistant bacteria in patients from India (3,4). We report the isolation of 1 community-acquired NDM-1-bearing Salmonella strain isolated from a child with acute diarrhea.

The Salmonella strain was isolated from the feces of an 11-monthold girl at Lishui Central Hospital, Zhejiang Province, China, on July 25, 2012. Six days before admission, a fever <40[degrees]C, accompanied by a cough, developed in the patient. Four days before admission, physical examination showed fine rales in both lungs. The leukocyte count was 8,900 cells/[micro]L, with 80% neutrophils. No obvious abnormalities were found on a chest radiograph.

The patient was given a diagnosis of acute bronchitis, and the condition was treated with parenteral cefoxitin for 3 days and parenteral piperacillin/tazobactam for 1 day, but fever persisted. Two days before admission, diarrhea (4-5 times/day with loose feces containing mucus and blood) developed. On admission day, fecal analysis showed 3-4 leukocytes and 1-3 erythrocytes per high-power field.

A Salmonella sp. was isolated from feces obtained at admission and identified as S. enterica subsp. enterica serovar Stanley by serotyping by the local Centers for Disease Control and Prevention.

The patient was then given a diagnosis of bacterial enteritis and received intravenous azithromycin and latamoxef. Fever and diarrhea resolved over the next 3 days. On the fifth day of hospitalization, a fecal culture was negative for Salmonella spp. and the patient was discharged. At a follow-up visit 3 months later, Salmonella spp. or other carbapenem-resistant bacteria were not isolated from feces samples from the patient or her grandmother and brother, who lived with her.

The patient and her family had not traveled to any country during the year, including countries with a high prevalence of NDM-1 producers. The patient was living in a small rural village in southern China and did not have a special diet. She was healthy before hospitalization for fever. She was born by cesarean section and did not have contacts with hospitalized patients.

MICs of antimicrobial drugs were determined by agar dilution and interpreted by using revised Clinical and Laboratory Standards Institute breakpoints (5). The Salmonella Stanley strain was resistant to all [beta]-lactam antimicrobial drugs tested, including cephalosporins and carbapenems, but susceptible to chloramphenicol, ciprofloxacin, tetracycline, and fosfomycin, and had azithromycin MICs of 4 [micro]g/mL (Table).

A modified Hodge test result for Salmonella strain Stanley was weakly positive. Production of metallo-[beta]-plactamase was detected by using an imipenem-EDTA double-disk synergy test. Carbapenamase-encoding genes, including [bla.sub.KPC-2'] [bla.sub.VIM], [bla.sub.IMP'] [bla.sub.SPM-1], [bla.sub.GIM-1], and [bla.sim-1'] were detected by using PCR as described (2). Only the [bla.sub.NDM-1] gene was detected (with primers 5'-GGCGGAATGGCT CATCACGA-3' and 5'-CGCAACA CAGCCTGACTTTC-3'). The PCR product sequence was consistent with that of NDM-1 (GenBank accession no. FN396876).

Conjugation experiments were conducted as described (6). Carbapenem resistance could be transferred from Salmonella strain Stanley to E. coli C600 Rif and K. pneumoniae 13883 Rifr at frequencies of 1 trans-conjugant per [approximately equal to] 1.0 x [10.sup.4] and 4.0 x [10.sup.7] bacterial cells, respectively, after exposure for 15 min.

Plasmid DNA was extracted by using a Plasmid Midi Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. Electrophoresis showed that donor and transconjugant strains had the same plasmid profile; both contained an [approximately equal to] 140-kb plasmid. A PCR-based method for plasmid replicon typing (7) indicated that the plasmid belonged to incompatibility group IncA/C.

Experiments of [bla.sub.NDM-1] stability in Salmonella spp., E. coli transconjugants, and K. pneumoniae transconjugants were conducted by using the method of Wang et al. (6). Twenty colonies were randomly selected every day during days 2-15. Only 2 colonies of the Salmonella Stanley strain lost carbapenem resistance; these colonies were collected on the second and ninth days of passage, respectively. Plasmids containing [bla.sub.NDM-1] were not present in these 2 colonies. No transconjugants lost carbapenem resistance during 14 days of passage.

Although Salmonella spp. have shown increased resistance to cephalosporins and quinolones, resistance to carbapenems is rare (3,4,8,9). Because of emerging resistance to traditionally recommended antimicrobial agents, azithromycin is increasingly used for treatment of invasive Salmonella spp. infections in children (10). The patient with carbapenem-resistant Salmonella infection and acute diarrhea was cured by treatment with azithromycin.

This report indicates ongoing spread of NDM-1-bearing Salmonella strains. If one considers the high conjugation frequency and stability of the IncA/C plasmid containing NDM-1 in Salmonella spp., one would conclude that it might increase spread of bacterial drug resistance. Prompt recognition of carbapenem-resistant Salmonella spp. and initiation of appropriate infection control measures are essential to avoid spread of these organisms.

References

(1.) Yong D, Toleman MA, Giske CG, Cho HS, Sundman K, Lee K, et al. Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. Antimicrob Agents Chemother. 2009;53:5046-54. http://dx.doi.org/10.1128/AAC.00774-09

(2.) Nordmann P, Poirel L, Walsh TR, Livermore DM. The emerging NDM carbapenemases. Trends Microbiol. 2011;19:588-95. http:// dx.doi.org/10.1016/j.tim.2011.09.005

(3.) Cabanes F, Lemant J, Picot S, Simac C, Cousty J, Jalin L, et al. Emergence of Klebsiella pneumoniae and Salmonella metallo-beta-lactamase (NDM-1) producers on Reunion Island. J Clin Microbiol. 2012;50:3812. http://dx.doi.org/10.1128/JCM.01029-12

(4.) Savard P, Gopinath R, Zhu W, Kitchel B, Rasheed JK, Tekle T, et al. First NDM positive Salmonella sp. strain identified in the United States. Antimicrob Agents Chemother. 2011;55:5957-8. http:// dx.doi.org/10.1128/AAC.05719-11

(5.) Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 22nd informational supplement. CLSI document M100-S22. Wayne (PA): The Institute; 2012.

(6.) Wang M, Tran JH, Jacoby GA, Zhang Y, Wang F, Hooper DC. Plasmid-mediated quinolone resistance in clinical isolates of Escherichia coli from Shanghai, China. Antimicrob Agents Chemother. 2003;47:2242-8. http://dx.doi. org/10.1128/AAC.47.7.2242-2248.2003

(7.) Johnson TJ, Lang KS. IncA/C plasmids: an emerging threat to human and animal health? Mob Genet Elements. 2012;2:558. http://dx.doi.org/10.4161/mge.19626

(8.) Su LH, Wu TL, Chiu CH. Development of carbapenem resistance during therapy for non-typhoid Salmonella infection. Clin Microbiol Infect. 2012;18:E91-4. http://dx.doi.org/10.1111j.1469 0691.2012.03767.x

(9.) Cui S, Li J, Sun Z, Hu C, Jin S, Guo Y, et al. Ciprofloxacin-resistant Salmonella enterica serotype Typhimurium, China. Emerg Infect Dis. 2008;14:493-5. http:// dx.doi.org/10.3201/eid1403.070857

(10.) Sjolund-Karlsson M, Joyce K, Blick enstaff K, Ball T, Haro J, Medalla FM, et al. Antimicrobial susceptibility to azithromycin among Salmonella enterica isolates from the United States. Antimi crob Agents Chemother. 2011;55:3985-9. http://dx.doi.org/10.1128/AAC.00590-11

(1) These authors contributed equally to this article.

Jinwei Huang, (1) Minghua Wang, (1) Hui Ding, Meiping Ye, Fupin Hu, Qinglan Guo, Xiaogang Xu, and Minggui Wang

Author affiliations: Huashan Hospital, Fudan University, Shanghai, China (J. Huang, M. Wang, F. Hu, Q. Guo, X. Xu, M. Wang); Lishui Central Hospital, Lishui, China (J. Huang, H. Ding); and Wenzhou Medical University, Wenzhou, China (M. Ye)

DOI: http://dx.doi.org/10.3201/eid1912.130051

Address for correspondence: Minggui Wang, Institute of Antibiotics, Huashan Hospital, Fudan University, 12 M. Wulumuqi Rd, Shanghai 200040, People's Republic of China, email: mgwang@fudan.edu.cn
Table. Antimicrobial drug susceptibility of Salmonella strain
Stanley and transconjugants containing New Delhi metallo-[beta]-
lactamase-1, China

                                     MIC, [micro]g/mL

                       Salmonella      Escherichia      E. coli C600
                         strain         coli C600      transconjugant
Drug                    Stanley

Piperacillin//
  tazobactam             >128/4            2/4             128/4
Ceftazidime               >128             0.25             >128
Cefotaxime                >128            <0.06             128
Latamoxef                  64              0.25              32
Cefepime                   16             <0.06              16
Imipenem                   8              <0.06              8
Meropenem                  4              <0.06              4
Fosfomycin               <0.06            <0.06            <0.06
Minocycline                2               0.5              0.5
Ciprofloxacin            <0.06            <0.06            <0.06
Chloramphenicol            2                4                4
Azithromycin               4                1                1
Trimethoprim/
  sulfamethoxazole       >152/8         0.3/0.015          >152/8

                            MIC, [micro]g/mL

                       Klebsiella     K. pneumoniae
                       pneumoniae         13883
Drug                     13883        transconjugant

Piperacillin//
  tazobactam              1/4             128/4
Ceftazidime               0.25             >128
Cefotaxime               <0.06             >128
Latamoxef                <0.06              32
Cefepime                 <0.06              16
Imipenem                 <0.06              16
Meropenem                <0.06              8
Fosfomycin               <0.06            <0.06
Minocycline                1               0.5
Ciprofloxacin            <0.06            <0.06
Chloramphenicol            4                4
Azithromycin               2                1
Trimethoprim/
  sulfamethoxazole      1.2/0.06          >152/8
COPYRIGHT 2013 U.S. National Center for Infectious Diseases
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2013 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:LETTERS
Author:Huang, Jinwei; Wang, Minghua; Ding, Hui; Ye, Meiping; Hu, Fupin; Guo, Qinglan; Xu, Xiaogang; Wang, M
Publication:Emerging Infectious Diseases
Article Type:Letter to the editor
Geographic Code:9CHIN
Date:Dec 1, 2013
Words:1499
Previous Article:Porcine epidemic diarrhea virus variants with high pathogenicity, China.
Next Article:Contagious caprine pleuropneumonia in endangered tibetan antelope, China, 2012.
Topics:

Terms of use | Copyright © 2017 Farlex, Inc. | Feedback | For webmasters