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Nervous system development in feeding and nonfeeding asteroid larvae and the early juvenile.

Introduction

The anatomy of the echinoderm larval nervous system (LNS) has been the subject of numerous investigations based on histochemistry, electron microscopy, and immunocytochemistry (reviews: Cobb, 1987; Nakajima et al., 2004a; Byrne et al., 2007). The most widely used and successful approach to investigate the anatomy of the LNS has involved immunocytochemistry using antibodies to a range of neurochemicals. In particular, antibodies to serotonin (5-HT). dopamine. GABA. and the echinoderm-specific neuropeptide GFNSALMFamide 1 (S1) have been used to visualize the LNS of planktotrophic larvae (Burke et al.. 2006; Chia et al., 1986; Bisgrove and Burke, 1987; Nakajima. 1987, 1988; Bisgrove and Raff. 1989; Nakajima et al.. 1993; Moss et al., 1994; Byrne et al., 1998, 2001, 2007; Chee and Byrne, 1999a, b; Cisternas et al., 2001; Falugi et al., 2002; Byrne and Cisternas, 2002; Cisternas and Byrne. 2003; Yaguchi and Katow, 2003; Katow et al., 2009). Recently, the pan-neural synaptotagmin B monoclonal antibody generated from the sea star radial nerve cord has been used to great effect in studies of the LNS of echinoids, holothuroids, and asteroids (Nakajima et al., 2004a, b; Burke et al., 2006; Nakano et al., 2006; Bishop and Burke. 2007; Katow et al., 2009) and the nervous system (NS) of adult asteroids (Saha et al., 2006; Murabe et al., 2008). Synaptotagmin is a calcium-binding protein localized in synaptic vesicles (Sudhof. 2002).

Asteroids with planktotrophic development have two larval stages: the feeding bipinnaria and the settlement-stage brachiolaria. Possession of the feeding bipinnaria larva is considered to represent the ancestral developmental mode for modern Asteroidea (review, Strathmann, 1975; Byrne, 2006; Raff and Byrne, 2006). The bipinnaria was lost in the evolution of lecithotrophic development (Byrne et al., 2001). Application of the neural markers, synaptotagmin and S1, reveals an extensive network of neurites and cell bodies in the ciliary band (CB) nerves, oral region, and digestive tract in the bipinnaria and in the attachment complex of the brachiolaria (Moss et al., 1994; Byrne and Cisternas, 2002; Nakajima et al, 2004a; Murabe et al., 2008). Here we used the synaptotagmin marker in a comparative study of the LNS of asterinid sea stars with planktotrophic and lecithotrophic modes of development. Development of Patiriella regularis through feeding bipinnaria and brachiolaria larvae represents the ancestral mode of development, while Meridastra calcar and Parvulastm exigua have lecithotrophic planktonic and benthic brachiolaria, respectively (Byrne, 2006). With an estimated divergence time of less than 10 Mya (Hart et al., 1997), the evolution of enhanced maternal provisioning in M. calcar and P. exigua has resulted in loss of the bipinnaria and associated feeding structures (Byrne et al., 2001; Byrne and Cisternas, 2002; Byrne, 2006; Prowse et at, 2008). In association with this loss, there is no trace of the extensive network of CB nerves characteristic of the bipinnaria (Byrne et al., 2001). However species with feeding and nonfeeding larvae all have the settlement- and metamorphic-stage larva, the brachiolaria. The brachiolaria has morphological specializations for benthic settlement--three brachia and the adhesive disc (Byrne et al., 2001; Byrne, 2006). The anatomy of the attachment complex in the planktonic larvae of P. regularis and M. calcar is similar in the presence of a long median brachium flanked by two small ones and a central adhesive disc. In contrast, the benthic brachiolaria of P. exigua has a hypertrophic attachment complex that creates the tripod larval form with three large muscular brachia equal in length and a large adhesive disc (Byrne, 2005). These are adaptations for permanent benthic attachment.

Here we document the distribution of synaptotagmin immunoreactive cells and the organization and morphology of the NS in the brachiolaria and juveniles of asterinids with different modes of development. This is the first use of this marker with lecithotrophic asteroid larvae. The primary objectives were to identify modifications of the LNS associated with evolution of lecithotrophic development in M. calcar and P. exigua within a robust phylogenetic context and to document the fate of the LNS through metamorphosis and development of the juvenile NS. A recent study of neurogenesis in echinoids revealed that the larval NS is anatomically connected to that of the juvenile in both feeding and nonfeeding larvae prior to metamorphosis, after which there is no trace of the LNS (Katow et al, 2009). Here we focused on the larval stage shared by asterinid species with feeding and nonfeeding larvae, the brachiolaria. Although the LNS of asteroid bipinnariae is well characterized, this stage in P. regularis is included here for comparative purposes as the second bipinnaria examined with the synaptotagmin marker.

In all animals, development of the body plan is closely tied to development of the nervous system (Holland, 2003). For echinoderms, which arose from a bilateral ancestor, the evolution of pentamery and the fivefold NS is not well understood. It is also not known how the bilateral LNS of echinoderms relates to the pentamerous central nervous system (CNS) of the adult. The change in distribution of IR through metamorphosis from the bilateral LNS to the radial CNS is documented to assess the relationship between these two systems in the Asteroidea.

Materials and Methods

Specimen collection and spawning

Adult individuals of Patiriella regularis (Verrill) were collected near Hobart, Tasmania (November, December 2004-2006), and adults of Parvulastra exigua (Lamark) and Meridastra calcar (Lamark) were collected near Sydney, New South Wales (May-November 2004-2007). Spawning was induced by placing the sea stars in filtered seawater (FSW: 1 [micro]m, Millipore) with [10.sup.-5] mol [1.sup.-1] 1-methyladenine. The gametes were released within 3-4 h and used for fertilization to establish larval cultures. For P. regularis, cultures were fed with Pavlova lutheri, Isochrysis galhana, or Chaetoceros muelleri at a cell concentration of 30000 [l.sup.-1] every second day. The FSW was changed by reverse filtration prior to feeding, Patiriella regularis larvae were reared to the juvenile stage (3 mon) at 20 [degrees] C in 25-1 containers with aeration. The nonfeeding lecithotrophic larvae of P. exigua and M. calcar were reared to the juvenile stage in petri dishes.

Immunocytochemistry

Larvae from P. regularis, P. exigua, and M. calcar were used for synaptotagmin immunocytochemistry using the 1E11 antibody (Nakajima et al, 2004a). The larvae were placed in baskets (1-ml) made from BEEM capsules capped with a 60-[micro]m-mesh lid. Larvae or juveniles (n = 30-50) were placed in each basket in 24-well culture dishes and rinsed with 0.22-[micro]m FSW (Millipore). After 15 min (M. calcar, P. exigua) or 45 min (P. regularis) in 7% Mg[Cl.sub.2] to reduce muscle contraction, the specimens were fixed in 4% paraformaldehyde in FSW for 10 min at room temperature (RT). After fixation, specimens were immediately processed for immunocytochemistry, and all steps (except where specified) were carried out at RT on a rocker to create a gentle agitation of the solutions. After fixation, the specimens were placed in cold 100% methanol at RT for 2 min following Nakajima et al. (2004a). The samples were then rinsed three times (10 min) in 0.1 mol [1.sup.-1] phosphate buffered saline (PBS) pH 8.2-8.3 to eliminate excess fixative. After preincubation for 1 h in 10% normal goat serum (Vector laboratories) diluted in PBS with 0.1% Tween 20 (PBST), the samples were rinsed three times (10 min) in PBST. Specimens were incubated in primary antibody (1E11) overnight at 4 [degrees]C. After three washes in PBST, the specimens were incubated in secondary antibody anti-mouse IgG goat tagged with Alexa fluor 594 (1:500 in PBST) for 2 h at RT, followed by a final rinse in PBST (3 X 10 min). After dehydration through an ethanol series to 100%, the specimens were cleared and stored in benzyl benzoate/benzyl alcohol (Sigma-Aldrich) 2:1 (v/v). Controls consisted of omitting the primary antibody and omitting the secondary antibody. The presence of background autofluorescence was also checked. Samples were stored in glass dishes in the dark at 4 [degrees]C to preserve the fluorescent signal. Specimens stored for several months showed no sign of fading when examined.

Specimen preparation for microscopy

For examination with an upright fluorescence microscope, immunostained specimens were placed on a slide in a drop of benzyl benzoate/benzyl alcohol, and the coverslips were mounted on small plasticine feet. For examination using an inverted microscope, the larvae were placed in a chamber made by sealing a coverslip to the underside of a hole cut in a glass slide.

Specimens were viewed using confocal or deconvolution microscopy. For confocal microscopy, an FV 1000 confocal system on a IX 81 inverted confocal microscope (Olympus, Japan) was used. The 488-nm line of the argon laser was used with a filter block to visualize paraformaldehyde-induced autofluorence. The 543-nm line of the HeNe laser was used to excite the Alexa fluor 594. Some specimens were examined with a Zeiss LSM 510 Meta inverted microscope. Excitation was achieved using the 488-nm diode laser (for autofiuorescence) and 561-nm diode laser (for Alexa fluor 594). For deconvolution microscopy, a Zeiss Axioplan 2 upright automated microscope (Carl Zeiss Germany) fitted with FITC (EX 480/40, 505LP, EM 535/50) and Texas Red (EX 560/55, 595LP, EM 645/75) filter sets were used.

Images captured on the Olympus confocal microscope were analyzed with Fluoview ver. 1.5. Images taken using the Zeiss were acquired with AxioVision ver. 4.0 image acquisition software. These software packages were used to construct extended focus projections of all serial sections.

For scanning electron microscopy, larvae were fixed for 1 h at RT in 2.5% glutaraldehyde in 0.22-[micro]m FSW, then rinsed in 2.5% NaHC03 (pH 7.2). The conventional secondary fixation step in osmium was deleted as in previous studies of larval structure (e.g., Nunes and Jangoux, 2007). The specimens were rinsed in distilled water, dehydrated in graded ethanols, critical-point-dried, mounted on stubs, sputter-coated, and viewed with a JOEL 35C scanning electron microscope.

For wax histology. P. exigua juveniles were fixed and decalcified in Bouin's fluid for 24 h, rinsed in distilled water, dehydrated in graded ethanols, and embedded in paraffin. Serial sections (7 [micro]m thick) stained with haematoxylin and eosin were photographed.

Results

Synaptotagmin immunoreactivity in the bipinnaria of Patiriella regularis

In the bipinnaria of P. regularis, synaptotagmin IR revealed an extensive larval nervous system (LNS) comprising an array of cell bodies and neurites along the length of the ciliary band (CB) nerve (Fig. 1A, C). The most prominent IR was localized along the pre- and postoral CB sections (Fig. 1 A, C). In the oral region, two clusters of cells corresponded to the adoral CB nerve and ganglion (Fig. 1A, B). The esophagus had also immunoreactive neurites (Fig. 1B). Immunoreactive cell bodies and neurites were positioned in the CB epithelium (Fig. 1C-H). In the preoral CB nerve, the cell bodies gave rise to processes that connected with the adjacent network of neurites in the pre-oral hood (Fig. 1C). The CB neurons were closely aligned along the basal region of the epithelium and were joined by basal processes (Fig. 1D). The cell bodies of neurons of the preoral CB nerve varied in shape. These cells included triangular cells with the basal region at the base of the CB epithelium, and these gave rise to an apical process that connected with the epithelial surface and basal processes that merged with the CB nerve (Fig. 1E). Round cells at the basal region of the epithelium did not have an immunoreactive apical region (Fig. 1F). The cell body of bipolar spindle-shaped cells was positioned in the mid-epithelial region. These spindle-shaped cells had a narrow apical region and gave rise to a basal neurite (Fig. 1G). In the post-oral CB nerve, some cell bodies were in a mid-epithelial position, had a round profile, and connected with the network of fibers adjacent to the CB (Fig. 1C). Some of these cells gave rise to several neurites and appeared multipolar (Fig. 1G).

Synaptotagmin immunoreactivity in the brachiolaria of Patiriella regularis

The brachiolaria of P. regularis has an attachment complex comprising the three brachia and the adhesive disc (Fig. 2A). The median brachium was covered by prominent adhesive papillae (Fig. 2B). Elongate spindle-shaped neurons extended through the epithelium of the adhesive papillae (Fig. 2F). At their base these cells gave rise to a process that merged with the basi-epithelial nerve plexus (Fig. 2F). Synaptotagmin IR was evident in the CB nerves that followed the contours of the bands in a manner similar to that we observed in the bipinnaria. Two lines of IR in the anterior projection coincided with the position of the CB on the dorsal and ventral sides (Fig. 2C, E). Neurites projecting between these two regions anatomically connected the ventral and dorsal sides in an anastomosing network of cell bodies and processes (Fig. 2E). The dorsal side of the brachium had neurites and a few cell bodies (Fig. 2E). Immmunoreacitivity was also evident in the esophagus (Fig. 2C).

As the larval body was resorbed during metamorphosis, the LNS degenerated. In advanced brachiolaria, IR became focused on the adhesive disc, forming an extensive nerve plexus (Fig. 2G-H). Transverse optical section of the adhesive disc revealed cell bodies located along the base of the disc (Fig. 21--J).

Synaptotagmin immunoreactivity in the brachiolaria of Meridastra calcar and Parvulastra exigua

The brachia of the lecithotrophic brachiolaria of M. calcar and P. exigua do not have adhesive papillae as seen in P. regularis. In these species, neurons were scattered fairly uniformly over the brachial surface, and IR was most prominent in the attachment complex (Fig. 3A, D-F). The neurons varied in shape and included spindle-shaped cells (Fig. 3F). Most of the cells had an elongate profile with an apical projection and a basal neurite that merged with the basi-epithelial nerve plexus along the basal lamina of the epidermis (Fig. 3F, H-I). This network of fibers spanned between the three brachia so the nervous tissue of these structures were connected (Fig. 3A, D-E). Immunoreactive cells were also present scattered across the general epithelium of the larval body (Fig. 3G). A few immunoreactive cells were also present in the adhesive disc (Fig. 3E).

As the brachiolariae developed. IR in the adhesive disc increased (Fig. 4A--B). In advanced larvae competent to metamorphose, the adhesive disc contained prominent elongate spindle-shaped neurons, similar to those seen in the brachial epithelium (Fig. 4B-C). Neurons in the adhesive disc and brachia merged with the basi-epithelial plexus underlying the entire attachment complex (Fig. 4C-D).

[FIGURE 4 OMITTED]

Synaptotagmin immunoreactivity in juveniles

Development of the juvenile NS was similar in the three species examined. In early juveniles, IR was first observed in the NS of the tube feet and more faintly in the circumoral nerve ring and radial nerve cords of the CNS (Fig. 5A). A network of neurites lined the podial epithelium (Fig. 5A-B). At the base of the tube foot where the podial NS joined with the radial nerve cords. IR decreased and merged with that in the developing nerve cord (Fig. 5D). Neurons were scattered across the surface of the tip of the tube feet and connected with a plexus at the base of the epithelium (Fig. 5E-F). Confocal sections revealed the presence of additional immunoreactive cells in a mid-epithelial position that gave rise to neurites that projected apically and basally (Fig. 5G). The processes from these cells connected with the basal plexus (Fig. 5C, G).

As the juveniles developed, strong IR was evident in the circumoral nerve ring and radial nerve cords, and the pattern of synaptotagmin localization reflected the histological organization of the asteroid CNS into the ectoneural and hyponeural tissue layers (Fig. 6A-E). Histological sections show the ectoneural system formed by an outer somatic layer of cell bodies positioned along the body wall epithelium and the inner axonal layer (Fig. 6A-B). Synaptotag-min-positive cell bodies were scattered along the outer somatic layer, indicating that not all cells in this tissue layer express this marker (Fig. 6D-E). Immunoreactivity in the axonal layer appeared tibrous and is likely to be localized to axons (Fig. 6D-E). A network of cells and neurites present in the inter-ambulacral region corresponded to the general body nerve plexus (Fig. 6F). The cells were irregularly shaped and gave rise to several neurites (Fig. 6G).

Discussion

Neurogenesis in asterinids with feeding larvae is divided into two distinct modules: the bipinnaria stage with its well-developed ciliary band (CB) nerves and the brachiolaria where a new set of neurons form in association with development of the attachment complex (Byrne et al., 2001). Evolution of nonfeeding development is associated with loss of the bipinnaria stage and all traces of the CB nerves (Byrne et al., 2001; Byrne and Cisternas, 2002). Similarly, evolution of nonfeeding development in sea urchins has resulted in loss of the CBs and associated nervous system (NS) (Byrne et al., 2007; Katow et al., 2009). The asterinid species investigated here all have the brachiolaria stages, which are morphologically similar among species with or without the preceding bipinnaria. This shows the conservation of the brachiolaria stage, which serves in benthic attachment, an obligatory phase in the transition from the planktonic to the benthic environments.

Synaptotagmin IR in the bipinnaria was seen in the CB nerves, adoral ganglia, and digestive tract, similar to that seen with peptidergic IR (S1) in Patiriella regularis and other asteroids and to synaptotagmin IR in Asterina pectinifera bipinnaria (Cisternas et al., 2001; Cisternas and Byrne. 2003; Nakajima et al., 2004a; Murabe et al., 2008). As seen here for P. regularis, the extensive plexus of neurites associated with the CB system and digestive tract is characteristic of echinoderm feeding larvae (Nakajima et al., 2004a, b; Nakano et al., 2006; Hirokawa et al., 2008). The distribution of the LNS in planktotrophic larvae is suggested to reflect its role in modulation of feeding and swimming (Lacalli, 1996; Katow et al., 2004, 2007). The cilia of the adoral CB carry food particles into the esophagus, which is followed by esophageal muscle contraction (Strathmann. 1975). The connection of neuronal cells to the CB nerve and adjacent neuronal network seen here with P. regularis is suggested to be important in coordination of ciliary activity for swimming and feeding (Chee and Byrne, 1999a). In P. regularis, cells of the CB nerve gave rise to neurites that connected to the adjacent neuronal network in the pre- and post-oral region; this arrangement was similar to that in A. pectinifera (Nakajima et al., 2004a). It is not known if these processes are axonal projections to effector cells or whether they are dendritic projections to sensory cells, as is generally the case for echinoderm LNS (Nakajima et al., 2004a; Bishop and Burke, 2007). The cell bodies of the immunoreactive neurons of the CB nerve varied in shape, a common feature of asteroid LNSs (Chee and Byrne, 1999a; Nakajima et al., 2004a). This variation is also reported for the cells of the serotonergic system of the larva of P. regularis (Chee and Byrne. 1999a). It is not known if these differences in cell shape represent different populations of neurons. In the anterior projection of the brachio-laria of P. regularis, both the distribution of neurites in the CB sectors and the neuronal network between the dorsal and ventral sides are similar to those described for A. pectinifera (Murabe et al., 2008). The 1EI1 immunoreactive paired lateral ganglia that compose part of the asteroid larval apical organ in A. pectinifera (Murabe et al., 2008) were not immunoreactive in the present study in P. regularis.

As in previous studies of nervous system development in nonfeeding asteroid larvae (Byrne et al., 2001; Byrne and Cisternas, 2002), Meridastra calcar and Parvulastra exigua did not have any trace of the LNS seen in the bipinnaria. Echinoids with nonfeeding larvae also lack the CB and the associated NS of the echinopluteus (Byrne et al., 2007; Katow et al., 2009). Our results suggest that development of the brachiolaria stage of P. regularis initiated a new neurogenic program as the attachment complex formed.

Regardless of the presence or absence of a feeding-stage larva, the synaptotagmin IR of the brachiolaria of the three species was strikingly similar. This provides insights into the aspects of the NS essential for the attachment stage in sea star development.

The distribution of synaptotagmin documented here and the peptidergic IR from other studies (Byrne et al., 2001; Byrne and Cisternas, 2002) shows that the organization of neuronal cells and the neural anatomy of the attachment complex are very .similar in these closely related asteroids. This similarity supports the notion of evolutionary conservation of the complex and its function regardless of developmental mode; it also suggests conservation of neurogenesis in attachment-stage larvae. An investigation of neural development earlier in embryogenesis in both M. calcar and P. exigua and. in a more detailed fashion, in P. regularis is needed to strengthen the hypothesis of neurogenic conservation.

In P. regularis, the apical region of the synaptotagmin immunoreactive neurons in the adhesive papillae connected to the surface of the epithelium, as noted for serotonergic cells in this larva (Chee and Byrne, 1999b). As in A. pectinifera, the 1E11 immunoreactive cells in the adhesive papillae of P. regularis are spindle-shaped and span the width of the papillae, with apical projections that extend to the surface (Murabe et al., 2008). These projections have been taken to suggest that these neurons have a sensory role in reception and transduction of chemical stimuli originating from the substrata, potentially communicating with the basal bundle of fibers (Murabe et al., 2008). Behavioral and ablation studies of settling larvae indicate that the adhesive papillae play a sensory role in substrate selection during metamorphosis (Barker, 1978; Murabe et al., 2007). After microsurgical removal of the papillae, brachiolaria fail to recognize settlement substrata (Murabe et al., 2007). The brachia of the three species investigated here have an abundance of serotonergic (Chee and Byrne, 1999b) and IE11 immunoreactive cells. In the larvae of P. regularis, these cells give rise to sensory-like apical projections (Byrne et al., 2001, 2007), as is also the case for the larvae of A. pectinifera (Murabe et al., 2008). Scanning electron microscopy revealed the presence of short sensory-like cilia on the surface of the brachia of P. regularis (Byrne et al., 2001). The brachia play an active role in substrate selection and benthic attachment, and so the sensory-like cilia may serve as surface receptors in association with this function (Byrne et al., 2001). Similar cells that likely serve a similar function have been observed in the primary podia of juvenile echinoids (Burke, 1980). Prior to settlement, the larvae of P, regularis and M. calcar move around on the substrate until the appropriate settlement signal is recognized. In response to the appropriate cue, the adhesive disc creates a stable attachment by secretion of adhesive material from secretory cells (Barker, 1978; Byrne et al., 2001).

The pattern of NS development in brachiolaria larvae of the three species reflected differences in the onset and length of benthic attachment. In P. exigua, the adhesive disc of early larvae had a well-developed NS on hatching. In P. regularis and M. calcar, by contrast, the NS of the adhesive disc lakes some days to develop. This difference reflects the fact that the brachiolaria of M. calcar and P. regularis are initially plank-tonic, whereas the brachiolaria of P. exigua is holobenthic. The adhesive disc of P. exigua exhibits heterochronic and hypertrophic development to be functional on hatching for permanent benthic attachment (Byrne, 2005). Here we present the first images of immunoreactive neurons in attachment-stage larvae undergoing metamorphosis. Interestingly, the NS of the adhesive disc of attached larvae of P. regularis at the final metamorphic stage was virtually identical to that seen in P. exigua. In both species, the adhesive disc of metamorphosing brachiolaria had immunoreactive cells at the base of the epithelium, and some extended from the base of the epithelium distally toward the apical surface. The nerve cells in the adhesive disc formed a plexus of fibers within the disc. During metamorphosis, fluorescence disappeared from the rest of the larval body as the LNS degenerated.

At metamorphosis, larval structures and tissues were absorbed in parallel with morphogenesis of the adult rudiment, and we noticed a temporal lapse in expression of synaptotagmin between the degeneration of the LNS and histogenesis of the juvenile NS. Investigation of peptidergic NS through metamorphosis in P. regularis also indicated a temporal lapse in the appearance of nerve-specific IR in CNS development (Byrne and Cisternas, 2002). Neither 1E11 nor S1 recognize undifferentiated nerve cells (Byrne and Cisternas, 2002; Murabe el al. 2008). The same "discontinuity" in neurogenesis and neuronal differentiation in development of the juvenile CNS was seen in in situ hybridization with the neurogenic genes engrailed where gene expression follows CNS histogenesis (Byrne et al., 2005). It appears that generation of the histological and anatomical architecture of the juvenile CNS precedes expression of neurochemicals (e.g., synaptotagmin and peptidergic neurochemicals) and neurogenic genes (engrailed) in differentiated neurons. The discontinuity in the presence of synaptotagmin immunoreactive cells at the metamorphosis observed herein suggests that the adult asteroid NS develops independently from the LNS, at least in the sense of material contribution. This is consistent with the hypothesis of independent evolution of the larval and adult NS in the last common ancestor of echinoderms (Beer et al, 2001; Nakano et al, 2006; Hirokawa et al., 2008). In juvenile development of A. pectinifera, the IF9 antibody, which recognizes a START (steroidogenic acute regulatory protein-related) lipid-transfer domain, localizes to an extensive network of fibers that may be neurons (Murabe et al., 2008). These fibers are suggested to contribute to the adult NS, but it is not clear if they are neurons.

From our findings in the developing juvenile, synaptotagmin IR was first strongly expressed in the NS of the tube feet, followed by IR in the CNS, similar to that seen with engrailed expression (Byrne et al., 2005). This may reflect the early role for the tube feet in benthic attachment after the adhesive disc is resorbed as the CNS differentiates post-settlement. The immunoreactive cell bodies seen here on the tip of the tube feet are likely to play a sensory role, as suggested for similar cells in echinoid podia (Burke, 1980; Burke et al.. 2006). The cells give rise to a neurite that connects with the dense bundle of processes in the basi-epithelial plexus. We found that the tube feet and CNS of asteroid juveniles, similar to those of newly metamophosed echinoids (Burke et al, 2006), are strongly 1E11 immunoreactive. In echinoids, however, there is no difference in the timing of onset of 1El1 - IR in the tube feet and CNS (Burke et al., 2006), which differs from what we observed with juvenile sea stars.

As the juvenile developed, synaptotagmin IR in the three species investigated here was similar to that seen in the adult CNS (Saha et at., 2006). In both juveniles and adults, synaptotagmin localizes to the circumoral nerve ring and radial nerve cords, and its distribution reflects the histological organization of the sea star CNS. The distribution of synaptotagmin IR is virtually identical to the pattern seen with peptidergic IR (Byrne and Cisternas, 2002). The axonal tissue layer of the CNS was strongly immunoreactive to 1E11. Along the periphery of the nerves in the cell body layers, only a few cells were immunoreactive.

Development of the three asterinid species investigated here converged in the formation of the juvenile. The structures and tissues supporting the LNS degenerated during metamorphosis and were resorbed, so it is likely that the neurons also degenerate. It appears that none of the LNS was incorporated into the adult NS, which conforms to observations with peptidergic and serotonergic markers (Byrne et al., 2001, 2007). Similar results were reported for echinoid and holothuroid metamorphosis (Chia and Burke, 1978; Burke et al., 2006; Nakano et al., 2006; Katow et al., 2009). Although it could be considered that the larval neurons that lose their 1E11 epitope re-differentiate later as juvenile neurons, this would require individual cells to migrate out of the degenerating larval domain into that of the developing juvenile.

Among the Bilateria, the change from a bilateral nervous system to a radial one in the Echinodermata represents an extreme case, and none of the larval nervous system appears to persist through metamorphosis. This contrasts with other Bilateria. In molluscs and insects, a subset or most of the larval nervous system persists through metamorphosis to serve as a scaffold for the juvenile nervous system or is remodeled as the brain is reorganized (Technau and Heinsenberg, 1982; Marois and Carew, 1990). Further studies of nervous system development during the critical metamor-phic transition in other echinoderm larvae will be important in understanding evolution of the unusual pentameral body plan of these bilaterians.

Acknowledgments

Yoko Nakajima (Keio University, Yokohama, Japan) kindly provided the lEll antibody. We thank Thomas Prowse and Drs, Louise Page and Devarajen Vaitilingon for assistance in collection of material and for provision of algal cultures. The microscopy facilities were provided by the Bosch Institute. The work was funded by a grant from the Australian Research Council (MB). We thank Dr. Svetlana Maslakova, Dr. Cory Bishop, and an anonymous reviewer for helpful comments.

Literature Cited

Barker, VI. F. 1978. Structure of the organs of attachment of brachiolaria larvae of Stichaster australis (Verrill) and Coscinasterias caktmaria (Gray). J. Exp. Mar. Biol. Ecol, 33: 1-36.

Beer, A.-J., C. Moss, and M. Thorndyke. 2001. Development of serotonin-like and SAI.MHamide-like imniunoreaclivity in the nervous system of the sea urchin Psammechinus mitiaris. Biol. Bull. 200: 268-280.

Bisgrove, B. W., and R. D. Burke. 1987. Development of the nervous system of the pluteus larvae of Strongytocentrohis droehachiensis. Cell Tissue Res. 248: 335-343.

Bisgrove, B. W., and R. A. Raff. 1989. Evolutionary conservation of the larval serotonergic nervous system in a direct developing sea urchin. Dev. Growth Differ. 31: 363-370.

Bishop, C. D., and R. D. Burke. 2007. Ontogeny of the holothurian larval nervous system: evolution of larval forms. Dev. Genes Evol. 217: 585-592.

Burke, R. D. 1980. Podial sensory receptors and the induction of metamorphosis in echinoids. J. Exp, Mar. Bio!. Ecol. 47: 223-234.

Burke, R. D., and A. W. Gibson. 1986. Cytological techniques for the study of larval echinoids with notes on methods for inducing metamorphosis. Methods Cell Biol. 27: 295-308.

Burke, R. D., L. VI. Angerer, M. R. Elphick, G. W. Humphrey, S. Yaguchi, T. Kiyama, S. Liang, X. Mu, C. Agca, W. H. Klein, et al. 2006. A genomic view of the sea urchin nervous system. Dev. Biol. 300: 434-460.

Byrne, M. 2005. Viviparity in the sea star Cryptasterina hystera (Asterinidae)--conserved and modified features in reproduction and development. Biol, Bull. 208: 81-91.

Byrne, M. 2006. Life history diversity and evolution in the Asterinidae. Integr. Camp. Biol. 46: 243-254.

Byrne, M., and P. Cisternas. 2002. Development and distribution of the peptidergic system in larval and adult PatirieUcr. comparison of sea star bilateral and radial nervous systems. J. Comp. Neurol. 451: 101-114.

Byrne, M., F. C. Chee, and P. Cisternas. 1998. Localisation of the neuropeptide S1 in the larval and adult nervous system of [he sea star Patirielia regularis. Pp. 187-191 in Echinoderm Research, M. D. C. Carnevali and F. Bonasoro. eds. A. A. Balkema, Rotterdam.

Byrne, M., P. Cisternas, and D. Koop. 2001. Evolution of larval form in the sea star genus Patirielia: conservation and change in the larval nervous system, Dev. Growth Differ. 43: 459-468.

Byrne, M., P. Cisternas, L. Elia, and B. Relf. 2005. Engrailed is expressed in larval development and in the radial nervous system of Patirielia sea stars. Dev. Genes Evol. 215: 608-617.

Byrne, M., Y. Nakajima, F. C. Chee, and R. D. Burke. 2007. Apical organs in echinodenn larvae: insights into larval evolution in the Ambulacraria. Evol. Dev. 9: 432-445.

Chee, F. C., and M. Byrne. 1999a. Development of the larval serotonergic nervous system in the sea star Patiriella regutaris as revealed by confocal imaging. Biol. Bull. 197: 123-131.

Chee, F. C., and M. Byrne. 1999b. Serotonin-like Immunoreactivity in the brachiolaria larvae of Patiriella regutaris. Invertebr. Reprod. Dev. 36: 111-115.

Chia, F.-S., and R. I). Burke. 1978. Echinoderm metamorphosis: fate of larval structures. Pp. 219-234 in Settlement and Metamorphosis of Marine Invertebrate Larvae, F.-S. Chia and M. E. Rice, eds. Elsevier, New York.

Chia, F.-S., R. D. Burke, R. Koss, P. V. Mladinov, and S. S. Rumrill. 1986. Fine structure of the doliolaria larva of the feather star Florometm serratissima. with emphasis on the nervous system. J. Mor-phol. 189: 99-120.

Cisternas, P., and M. Byrne. 2003. Peptidergic and serotonergic immunoreactivity in the metamorphosing ophiopluteus of Ophiactis resiliens (Echinodermaia, Ophiuroidea). Invertebr. Biol. 122: 177-185.

Cisternas, P., P. Selvakumaraswamy, and M. Byrne. 2001. Localisation of the neuropeptide S1 in an ophiuroid larva. Pp. 239-242 in Echinoderms 2000, M. Barker, ed. Swets and Seitlinger, Rotterdam.

Cobb, J. L. S. 1987. Neurobiology of the Echinodermata. Pp. 483-525 in Invertebrate Nervous Systems, M. A. Ali. ed. Plenum Press, New York.

Falugi, C, A. Diaspro, C. Angelini, M. L, Pedrotti, M. Raimondo, and M. Robello. 2002. Three-dimensional mapping of cholinergic molecules by confocal laser scanning microscopy in sea urchin larvae. Micron 33: 233-239.

Hart, M. W., M. Byrne, and M. J. Smith. 1997. Molecular phyiogenetic analysis of life-history evolution in asterinid starfish. Evolution 51: 1848-1861.

Hirokawa, T., M. Komatsu, and Y. Nakajima. 2008. Development of the nervous system in the brittle star Amphipholis kochii. Dev. Genes Evol. 218: 15-21.

Holland, N. D. 2003. Early central nervous system evolution: an era of skin brains? Nat. Rev. Neurosci. 4: 617-627.

Katow, H., S. Yaguchi, M. Kiyomoto, and M. Washio. 2004. The 5-HT receptor cell is a new member of secondary mesenchyme cell descendants and forms a major blastocoelar network in sea urchin larvae. Mech. Dev. 121: 325-337.

Katow, H., S. Yaguchi, and K. Kyozuka. 2007. Serotonin stimulates [[Ca.sup.2+].sub.i] elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel. J. Exp. Biol. 210: 403-412.

Katow, H., L, Elia, and M. Byrne. 2009. Development of nervous system to metamorphosis in feeding and non-feeding echinoid larvae, the transition from bilateral to radial symmetry. Dev. Genes Evol. 219: 67-77.

Lacalli. T. C. 1996. Mesodermal pattern and pattern repeats in the starfish bipinnaria larvae, and related patterns in other deuterostome larvae and chordates. Philos. Trans. R. Soc. Lond. B Biol. Sci. 351: 1737-1758.

Marois, R., and T. J. Carew. 1990. The gastropod nervous system in metamorphosis. J. Neurobiol. 21: 1053-1071.

Moss, C., R. D. Burke, and M. C. Thorndyke. 1994. Immunocytochemical localization of the neuropeptide SI and serotonin in larvae of the starfish Pisaxter ochraceus and Asterias rubens. J. Mar. Biol. Assoc. UK 74: 61-71.

Murabe, N., H. Hatoyama, M. Komatsu, H. Kaneko, and Y. Nakajima. 2007. Adhesive papillae on the brachiolar arms of brachiolaria larvae in two starfishes, Asterina pectinifera and Asterias amurensis, are sensors for metamorphic inducing factorfsl. Dev. Growth Differ. 49: 647-656.

Murabe, N., H. Hatoyama, S. Hase, M. Komatsu, R. D. Burke, H. Kaneko, and Y. Nakajima. 2008. Neural architecture of the brachiolaria larva of the starfish, Asterina pectinifera. J. Comp. Neurol. 509: 271-282.

Nakajima, Y, 1987. Developmental study on nervous system of Hemicentrotus puleherrimus larva. Dev. Growth Differ. 29: 408.

Nakajima, Y. 1988. Serotonergic nerve ceils of starfish larvae. Pp. 235-239 in Echinoderm Biology, R. D. Burke, P. V. Mladenov, P. Lambert, and R. L. Parsley, eds. A.A. Balkema. Rotterdam.

Nakajima, Y., R. D. Burke, and Y. Noda. 1993. The structure and development of the apical ganglion in the sea urchin pluteus larvae of Strongylocentrotus droebachensis and Mesipilia globulus. Dev. Growth Differ. 35: 531-538.

Nakajima, Y., H. Kaneko, G. Murray, and R. D, Burke. 2004a. Divergent patterns of neural development in larval echinoids and asteroids. Evol. Dev. 6: 95-104.

Nakajima, Y., T. Humphreys, H. Kaneko, and K. Tagawa. 2004b. Development and neural organization of the tornaria larva of the Hawaiian hemichordate. Ptychodera fiava. Zool. Sci. 21: 69-78.

Nakano, H., N. Murabe, S. Amemiya, and Y. Nakajima. 2006. Nervous system development of the sea cucumber Stichopus japonicus. Dev. Biol. 292: 205-212.

Nunes, C. De A. P., and M. Jangoux. 2007. Larval growth and perimetamorphosis in the echinoid Echinocardiwv cordalum (Echinoder-mata): the spatangoid way to become a sea urchin. Zoomorphology 126: 103-119.

Prowse, T., M. Sewell, and M. Byrne. 2008. Fuels for development: Evolution of maternal provisioning in asterinid sea stars. Mar. Biol. 153: 337-349.

Raff, R. A., and M. Byrne. 2006. The active evolutionary lives of echinoderm larvae. Heredity 97: 244-252.

Saha, A. K., M. Tamori, M. Inoue, Y. Nakajima, and T. Motokawa. 2006. NGIWYamide-induced contraction of tube feet and distribution of NGIWYamide-like immunoreactivity in nerves of the starfish Asterina pectinifera. Zool. Sci. 23: 627-632.

Strathmann, R. R. 1975. Larval feeding in echinoderms. Am. Zool. 15: 717-730.

Sudhof, T. C. 2002. Synaptotagmins: why so many? J. Biol. Chetm. 277: 7629-7632.

Technau, G., and M. Heisenberg. 1982. Neural reorganization during metamorphosis of the corpora pedunculata in Drosophila melanogaster. Nature 295: 405-407.

Yaguchi, S., and H. Katow. 2003. Expression of tryptophan 5-hydraxylase gene during sea urchin neurogenesis and role of serotonergic nervous system in larval behavior. J. Comp Neurol. 466: 219-229.

LAURA ELIA*, PAULINA SELVAKUMARASWAMY, AND MARIA BYRNE

Discipline of Anatomy and Histology, Bosch Institute, F13, University of Sydney. NSW 2006, Australia

Received 31 October; accepted If) April 2009.

* To whom correspondence should he addressed. E-mail: elia@anatomy.usyd.edu.au

Abbreviations; CB. ciliary band; IR. immunoreactivity: LNS. larval nervous system; NS, nervous system.
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Author:Elia, Laura; Selvakumaraswamy, Paulina; Byrne, Maria
Publication:The Biological Bulletin
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Geographic Code:8AUST
Date:Jun 1, 2009
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