Nasopharyngeal Gangliocytic Paraganglioma: A Case Report With Emphasis on Histogenesis.
REPORT OF A CASE
A 44-year-old woman presented with daily headaches and photophobia; her symptoms were worse in the evening and incompletely controlled with various medications. She also complained of pressure and fullness in her throat and difficulty swallowing. Magnetic resonance imaging revealed an enhancing mass projecting posteriorly off the nasal septum and extending through the floor of the left sphenoid compartment near the midline. A follow-up computed tomographic scan indicated expansion of the posterior septum, with erosion of the clivus leading superiorly into the sphenoid sinus. There was no intracranial extension. Her past medical history was unremarkable except for hypertension. An endoscopic biopsy of the mass was interpreted as a GE and a complete surgical excision of the mass followed.
The completely excised mass consisted of multiple fragments of gray-brown soft tissue measuring 2.0 x 1.5 x 0.5 cm in aggregate.
Microscopically, the tumor was composed of a haphazard admixture of 3 elements: mononuclear round to polygonal cells arranged in a nesting pattern with admixed multinucleated ganglion-like cells and fascicles of spindle cells, which were more prominent at the periphery of the tumor. There was focal cytologic atypia in both the mononuclear and multinucleated giant cells; however, no necrosis or mitotic figures were noted (Figure 1).
Sections of formalin-fixed, paraffin-embedded tissue were stained for various antibodies by the immunoperoxidase method. AH immunohistochemical stains were performed on a TechMate 1000 automated immunostainer (Biotek Solutions, Inc, Santa Barbara, Calif). The antibodies and their sources and dilutions are described in the Table. The results of the immunohistochemical stains are summarized in the Table. There was diffuse positive reactivity for chromogranin and synaptophysin (Figure 2), while vasoactive intestinal peptide reactivity was focal. The mononuclear neoplastic cells were positive for chromogranin and synaptophysin, whereas the ganglion-like cells were negative for these markers. Focal staining for cytokeratin (AE1/AE3) was demonstrated in the mononuclear cells. The fascicles of spindle cells were positive for S100 protein (Figure 3). Scattered S100positive sustentacular cells were seen at the periphery of the cell nests. Immunoreactivity for pancreatic polypeptide and somatostatin stains was negative.
Immunohistochemical Profile of the Various Components(*) Antibody Source (Dilution)([dagger]) Chromogranin Dako (1:700) Synaptophysin Dako (1:100) NSE Dako (1:4000) S100 Dako (1:4000) VIP BioGenex (1:7000) Pancreatic polypeptide Dako (1:12 000) Cytokeratin (AE1/AE3) Signet (1:800) Somatostatin Dako (1:5000) Mononuclear Round to Antibody Polygonal Cells Chromogranin + + Synaptophysin + NSE - S100 - VIP + Pancreatic polypeptide - Cytokeratin (AE1/AE3) + Somatostatin - Antibody Ganglion-like Cells Spindle Cells Chromogranin - - Synaptophysin - - NSE + - S100 - + VIP + + Pancreatic polypeptide - - Cytokeratin (AE1/AE3) - - Somatostatin - - (*) NSE indicates neuron-specific enolase; VIP, vasoactive intestinal peptide. Reactions graded as ++, strongly positive; +, positive; and - negative. ([dagger]) Dako Corporation, Carpinteria, Calif; BioGenex Laboratories, San Ramon, Calif; and Signet Laboratories Inc, Dedham, Mass.
Gangliocytic paragangliomas are not exclusive to the periampullary region, as was once thought. In addition to the classic periampullary location, GPs have been reported in the pylorus, jejunum, and appendix. One case was associated with a duodenal diverticulum. Some GPs have been associated with neurofibromatosis. Most cases range from 1 to 3 cm, although a 10-cm example has been reported. Although the majority of GPs behave in a benign fashion, rare examples of malignant GPs have been documented. The metastases usually contain the endocrine component of the tumor.
We recently encountered a case of GP of the periampullary region that metastasized to 3 regional lymph nodes. The histologic appearance varies from case to case, even in different regions of the same tumor. Three cell types--epithelioid, ganglion-like, and spindle--are present in varying proportions in a haphazard distribution. Some cases exhibit a predominance of the spindle cell component, resembling a ganglioneuroma, while others have largely epithelioid elements, resembling a paraganglioma or carcinoid tumor. Occasionally, the enlarged nuclei of the ganglion-like cells, as in our case, may suggest a carcinoma.
The cell of origin of GP has been a matter of controversy. The tumor consists of both neuroectodermally derived elements (Schwann-like spindle cells, neurons, and ganglion cells) and an endodermally derived component (neuroendocrine cells). The focal keratin reactivity in the epithelioid cells favors a relationship to carcinoid tumors; however, the absence of cytokeratin in many epithelioid areas and the presence of S100-positive spindle cells, analogous to sustentacular cells, support a relationship to a paraganglioma. Perrone et al postulated that the tumor is derived from Van Campenhout endodermal-neuroectodermal complexes in the ventral primordium of the pancreas. These complexes are embryologic structures found in the pancreas of humans as well as some animals. They are composed of islet cells and branches of sympathetic nerves, including ganglion cells. In the cases reported to date, the consistent localization of the GP along the route traveled by the ventral pancreatic primordium as it rotates around the duodenum, coupled with its reactivity for pancreatic polypeptide, was the basis for the postulated pancreatic origin of this lesion. Additionally, immunohistochemical reactivity for a wide array of endocrine products has been variously described in GPs, for example, pancreatic polypeptide, calcitonin, cholecystokinin, somatostatin, gastrin, leu-enkephalin, met-enkephalin, serotonin, substance P, and vasoactive intestinal peptide. However, there are several pitfalls to this histogenetic theory. Kermarec et al noted that if the pancreatic origin were to be true, then at least a few cases should have arisen in the pancreas proper, a site where a GP has yet to be described. Also, the pancreatic theory falls to explain the origin of the 2 neuroectodermal elements (ie, Schwann-like spindle cell and the ganglion cell) as an intrinsic part of GP.
Immunoreactivity for pancreatic polypeptide in the majority of GPs is a feature considered supportive of their pancreatic origin; however, immunoreactivity of pancreatic polypeptide is not specific for pancreatic endocrine phenotype. Immunoreactivity for pancreatic polypeptide has been demonstrated in a host of both neoplastic and nonneoplastic conditions of the pancreas, as well as in extrapancreatic tissues. In addition to carcinoids of the lung, rectum, common bile duct, and appendix, immunohistochemical staining for pancreatic polypeptide has been described in some cortical neurons of the brain, in epidermal Merkel cells of psoriatic skin lesions, and in specialized epithelium such as Barrett esophagus. Eeden et al recently described a GP of the appendix in which the presence of ectopic pancreatic tissue was ruled out. Our case of nasopharyngeal GP provides additional evidence arguing against the pancreatic origin of GP.
Recent studies have shown that stem cells maintained in adult organs, under certain conditions, are capable of differentiation along a variety of cell lines. Adult neural stem cells possess a wider differentiation repertoire than previously recognized. In irradiated adult mice, neural stem cells from the adult forebrain were shown to produce blood cells and repopulate the hematopoietic system. Marrow stem cells, when transplanted to the brain, have been shown to generate astrocytes. It is clear that, depending on their microenvironment, stem cell populations in adult organs are able to differentiate along many different cell lines. This suggests that adult stem cells can potentially differentiate along nonnative cell lineages. We believe that the origin of the GP is best explained on the basis of the totipotential nature of adult stem cells that are capable of exhibiting differentiation along diverse cellular lineages.
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Accepted for publication February 1, 2001.
From the Division of Anatomic Pathology, University of Texas Southwestern Medical Center, Dallas.
Reprints: Jorge Albores-Saavedra, MD, Department of Pathology, University of Texas, Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9073 (e-mail: Albores.jorge@pathology. swmed.edu).
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|Author:||Sinkre, Prasanna; Lindberg, Guy; Albores-Saavedra, Jorge|
|Publication:||Archives of Pathology & Laboratory Medicine|
|Date:||Aug 1, 2001|
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