NANO-CT DEVICE SUCCESSFULLY TESTED.
During a CT analysis, the object under investigation is x- rayed and a detector measures the respective amount of radiation absorbed from various angles. Three-dimensional images of the inside of the object can be constructed based on several such measurements. Up until now, however, the technology reached its limits when it came to objects as small as the tiny, 0.4 millimeter long legs of the velvet worm (Onychophora).
High-resolution images of this magnitude required radiation from particle accelerators, yet there are only a few dozen such facilities in Europe. Approaches suitable for the typical laboratory still had to struggle with low resolutions, or the samples investigated had to be made of certain materials and could not exceed a certain size. The reason was often the use of x-ray optics. Put simply, x-ray optics focus x-ray radiation similar to the way optical lenses focus light but they also have several limitations.
The TUM Nano-CT system is based on a newly developed x-ray source, which generates a particularly focused beam, without relying on x-ray optics. In combination with an extremely low-noise detector, the device produces images that approach the resolution possible with a scanning electron microscope, while also capturing structures under the surface of the object under investigation. Our system has decisive advantages compared to CTs using x-ray optics, says TUM scientist Mark MAaAaAeA lle lead author of the PNAS article. We can make tomographies of significantly larger samples and we are more flexible in terms of the materials that can be investigated. Exactly these properties were ideal for the team led by Prof. Georg Mayer, head of the Department of Zoology at the University of Kassel. The scientists investigate the evolutionary origin of arthropods, including for example insects, spiders and crustaceans. Their current research, however, focuses on velvet worms (onychophorans), which can be thought of as worms with legs and are closely related to arthropods. Some velvet worm species can grow to a length of up to 20 centimeters, while others do not exceed 1 centimeter. The exact zoological classification of these ancient animals is still a matter of controversy; presumably, they share a common ancestor with arthropods and tardigrades (water bears).
In contrast to arthropods, onychophorans do not have segmented limbs, as is also the case with their presumed common fossil ancestors, says Georg Mayer. The investigation of the functional anatomy of the velvet worm's legs plays a key role in determining how the segmented limbs of the arthropods evolved. The Nano-CT images make it possible to investigate the individual muscle strands of the velvet worm leg. The team from Kassel plans to publish detailed results in the next few months. But they are already convinced about one thing: The Nano-CT device has passed its first practical test.
Like so many other imaging instruments, the Nano-CT system was developed and installed at the Munich School of BioEngineering (MSB). This TUM interdisciplinary research center is Europe's thematically most comprehensive university facility for the intersection of medicine, engineering sciences and natural sciences. Our goal in the development of the Nano-CT system is not only to be able to investigate biological samples, such as the leg of the velvet worm, says Franz Pfeiffer, TUM Professor for Biomedical Physics, Director of the MSB, and a Fellow at the TUM Institute for Advanced Study (TUM-IAS).
In the future, this technology will also make biomedical investigations possible. Thus, for example, we will be able to examine tissue samples to clarify whether or not a tumor is malignant. A non-destructive and three-dimensional image of the tissue with a resolution like that of the Nano-CT.
Technical University of Munich (TUM) is one of Europe's leading research universities, with more than 550 professors, around 10,000 academic and non-academic staff, and 41,000 students. Its focus areas are the engineering sciences, natural sciences, life sciences and medicine, combined with economic and social sciences. TUM acts as an entrepreneurial university that promotes talents and creates value for society. In that it profits from having strong partners in science and industry. It is represented worldwide with a campus in Singapore as well as offices in Beijing, Brussels, Cairo, Mumbai, San Francisco, and SAaAaAeALo Paul Nobel Prize winners and inventors such as Rudolf Diesel, Carl von Linde, and Rudolf MAaAaAeA [sz]bauer have done research at TUM. In 2006 and 2012 won recognition as a German "Excellence University." In international rankings, TUM regularly places among the best universities in Germany.
For more information, visit http://www.bioengineering.tum.de/ or call +49 (89) 289 12551.