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Mycoplasma ovipneumoniae in Wildlife Species beyond Subfamily Caprinae.

Mycoplasma ovipneumoniae was identified in 1 VI Queensland, Australia, in 1972 as an infectious agent associated with pneumonia in domestic sheep (Ovis aries) (1). Since then, it has most frequently been identified in healthy and diseased domestic sheep, domestic goats (Capra aegagrus hircus), and bighorn sheep (Ovis canadensis). Although M. ovipneumoniae was identified in respiratory disease outbreaks in bighorn sheep as early as 1980 (2), the past decade has brought it under scrutiny because of evidence supporting its association with bighorn sheep pneumonia in western North America (3). Because most reports have described this bacterium in sheep and goats, and fewer in muskoxen (Ovibosmoschatus) (4), some have concluded that M. ovipneumoniae is specific to the subfamily Caprinae (5) or has a host range limited to Caprinae (6), despite publications describing M. ovipneumoniae in non-Caprinae species, including Beira antelope (Dorcatragus megalotis) with respiratory disease in Qatar (7) and in 9 cattle (Bos taurus) in Colorado, USA (8). Unfortunately, description of the method(s) used to identify M. ovipneumoniae in those reports was limited to stating the use of PCR with no supporting sequence data.

In general, definitive claims of host range restrictions are absent from mycoplasma literature, because "assumptions about restricted host range of mycoplasmas, based on the host from which they were first or frequently isolated, are usually made in the context of nearly complete absence of representative sampling of the vast majority of potential vertebrate hosts" (9). In addition to insufficient sampling of potential hosts, the fastidious and variably culturable nature of M. ovipneumoniae often requires molecular techniques for identification. We used molecular techniques to analyze multiple species from the subfamily Capreolinae for the presence of M. ovipneumoniae.

During July 2017-January 2018, the US Department of Agriculture Agricultural Research Service in Pullman, WA, USA, received nasal swab samples from 230 moose (Alces alces) and 243 caribou (Rangifer tarandus) from Alaska and 5 mule deer (Odocoileus hemionus) from Arizona (online Technical Appendix, https://wwwnc.cdc.gov/ EID/article/24/12/18-0632-Techapp1.pdf). Also received in February 2018 was an isolate of M. ovipneumoniae that had been cultured by Newport Laboratories (Worthington, MN, USA) from lung tissue from a white-tailed deer (Odocoileus virginianus) that died during a pneumonia outbreak at a captive facility in the upper Midwest region of the United States in 2016. We extracted DNA from swab samples and from the white-tailed deer isolate, performed PCR using a modified published PCR method (10) to amplify part of the 16S rRNA gene, and sequenced amplicons of the correct size (online Technical Appendix). Forward and reverse sequences were merged, manually inspected for errors, and trimmed to 290 bp using Sequencher 5.2.2 (Gene Codes, Ann Arbor, MI, USA) corresponding to a 103-392-bp region of the 16S rRNA gene of type strain Y98 obtained from American Type Culture Collection (ATCC; Manassas, VA, USA). Sequences were blastn queried (https://www. ncbi.nlm.nih.gov/BLAST/) and identified as M. ovipneumoniae at 100% coverage and [greater than or equal to] 97% identity to Y98 (GenBank accession no. NR_025989.1). The analyzed region represents the most divergent region of the 16S rRNA gene among strains of M. ovipneumoniae and between Mycoplasma spp. of the highest percentage identity to M. ovipneumoniae (online Technical Appendix).

We detected M. ovipneumoniae in 6 moose (2.6%), including 3 from 2 captive facilities and 3 free-ranging; 5 free-ranging caribou (2.1%); and 2 of 5 mule deer, 1 of which was coughing and had nasal discharge at the time of sample collection. The identity of the lung isolate, cultured from the white-tailed deer that had died from pneumonia, was confirmed as M. ovipneumoniae. For sequence comparison, we generated a percent identity matrix with the M. ovipneumoniae sequences from the Capreolinae species, nasal swabs collected from 2 healthy M. ovipneumoniae-positive wild sheep (online Technical Appendix), Y98, and bacteria of the closest identity to Y98 and sequences generated in this study based on blastn queries (M. dispar, M. hyopneumoniae, and M. flocculare) (online Technical Appendix). The percent identity matrix revealed 2 groupings of M. ovipneumoniae and illustrates the divergence from the other Mycoplasma spp. of closest identity to M. ovipneumoniae. Sample sequences have been submitted to GenBank (online Technical Appendix).

This report describes M. ovipneumoniae carriage in multiple members of the subfamily Capreolinae (moose, caribou, and mule deer), and emergence of M. ovipneumoniae-associated respiratory disease in deer. These findings are of importance to epidemiologic investigations because current dogma regarding host specificity may dissuade laboratories from pursuing identification of this fastidious bacterium in hosts beyond the subfamily Caprinae. Improved diagnostic methods to increase detection sensitivity are warranted based on information provided in this report (online Technical Appendix). Full-length genome sequencing and phylogenetic analysis of M. ovipneumoniae isolates are necessary next steps in inferring evolutionary relationships and origin of this bacterium in identified host species.

Acknowledgments

We thank Nicholas P. Durfee and Paige Grossman for technical support, Eric Roalson for assistance with sequence analysis, and Donald P. Knowles for final critical manuscript review. We acknowledge the following individuals and agencies for providing wildlife samples: Sara Longson, Anne Crane, John Crouse, Dominic Demma, Torsten Bentzen, Tony Hollis, Lincoln Parrett, Jason Caikoski, Warren Hansen, and the wildlife biologists and technicians at the Alaska Department of Fish and Game; Navajo Nation Zoological and Botanical Park-Navajo Nation Department of Fish and Wildlife; Ed Klein and Dan Love, Colorado Department of Agriculture; and Anthony Madrid and Lindsey Hansen, US Department of Agriculture Forest Service, San Juan National Forest.

Financial support for this project was provided by the US Department of Agriculture Agricultural Research Service, CRIS Project funds 2090-32000-036-00D.

Dr. Highland is a veterinary medical officer at the US Department of Agriculture Agricultural Research Service Animal Disease Research Unit in Pullman, WA, USA, and is an adjunct faculty member in the department of veterinary microbiology and pathology and the School for Global Animal Health at Washington State University. Her research interests include infectious diseases of small ruminants, with special focus on respiratory disease agents of domestic and wildlife species.

References

(1.) Carmichael LE, St George TD, Sullivan ND, Horsfall N. Isolation, propagation, and characterization studies of an ovine Mycoplasma responsible for proliferative interstitial pneumonia. Cornell Vet. 1972;62:654-79.

(2.) Bunch TD. Chronic sinusitis in desert bighorn sheep in northwestern Arizona. In: Douglas CL, Krausman PR, Leslie DM, editors. Desert Bighorn Council meeting 1985. Las Vegas (NV, USA): the Council; 1985. p. 1-3.

(3.) Besser TE, Highland MA, Baker K, Cassirer EF, Anderson NJ, Ramsey JM, et al. Causes of pneumonia epizootics among bighorn sheep, Western United States, 2008-2010. Emerg Infect Dis. 2012;18:406-14. http://dx.doi.org/10.3201/eid1803.111554

(4.) Handeland K, Tengs T, Kokotovic B, Vikoren T, Ayling RD, Bergsj0 B, et al. Mycoplasma ovipneumoniae--a primary cause of severe pneumonia epizootics in the Norwegian muskox (Ovibos moschatus) population. PLoS One. 2014;9:e106116. http://dx.doi.org/10.1371/journal.pone.0106116

(5.) Cassirer EF, Manlove KR, Plowright RK, Besser TE. Evidence for strain-specific immunity to pneumonia in bighorn sheep. Journal Wildl Manag. 2017;81:133-43. http://dx.doi.org/10.1002/jwmg.21172

(6.) Besser TE, Cassirer EF, Potter KA, Foreyt WJ. Exposure of bighorn sheep to domestic goats colonized with Mycoplasma ovipneumoniae induces sub-lethal pneumonia. PLoS One. 2017;12:e0178707. http://dx.doi.org/10.1371/journal.pone.0178707

(7.) Gull JM, Hebel C, Deb A, Arif A, Clauss M, Hatt JM, et al. Blood values of captive beira antelope (Dorcatragus megalotis) prior to and during an outbreak of fibrinous pleuropneumonia syndrome (FPPS). J Zoo Wildl Med. 2014;45:735-43. http://dx.doi.org/10.1638/2013-0073.1

(8.) Wolfe LL, Diamond B, Spraker TR, Sirochman MA, Walsh DP, Machin CM, et al. A bighorn sheep die-off in southern Colorado involving a Pasteurellaceae strain that may have originated from syntopic cattle. J Wildl Dis. 2010;46:1262-8. http://dx.doi.org/10.7589/0090-3558-46A1262

(9.) Brown DR, Zacher LA, Wendland LD, Brown MB. Emerging mycoplasmoses in wildlife. In: Blanchard A, Browning GF, editors. Mycoplasmas: molecular biology pathogenicity and strategies for control. Norfolk (UK): Horizon Bioscience; 2005. p. 382-414.

(10.) McAuliffe L, Hatchell FM, Ayling RD, King AI, Nicholas RA. Detection of Mycoplasma ovipneumoniae in Pasteurellavaccinated sheep flocks with respiratory disease in England. Vet Rec. 2003;153:687-8. http://dx.doi.org/10.1136/vr.153.22.687

Address for correspondence: Margaret A. Highland, US Department of Agriculture, Animal Disease Research Unit, Agricultural Research Service, ADBF 3003, Washington State University, Pullman, WA 99164, USA; email: maggie.highland@ars.usda.gov

Author affiliations: Washington Animal Disease Diagnostic Laboratory, Pullman, Washington, USA (M.A. Highland); US Department of Agriculture, Pullman (M.A. Highland, D.R. Herndon); Navajo Technical University, Crownpoint, New Mexico, USA (S.C. Bender); Barron Veterinary Clinic, Barron, Wisconsin, USA (L. Hansen); Alaska Department of Environmental Conservation, Anchorage, Alaska, USA (R.F. Gerlach); Alaska Department of Fish and Game, Fairbanks, Alaska (K.B. Beckmen)

DOI: https://doi.org/10.3201/eid2412.180632
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Article Details
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Title Annotation:RESEARCH LETTERS
Author:Highland, Margaret A.; Herndon, David R.; Bender, Scott C.; Hansen, Lisa; Gerlach, Robert F.; Beckme
Publication:Emerging Infectious Diseases
Article Type:Report
Date:Dec 1, 2018
Words:1490
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