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Mycobacterium BCG. (Senior Division).

This research was designed to study the effect of a recombinant form of Mycobacterium BCG, in which a fluorescent gene has been added to cultured macrophage and dendritic bone marrow derived cells. Using sterile technique, a culture titration was performed from stock bacteria to determine bacteria c.f.u. per ml. Bones removed from pathogenic-free mice were used to extract bone marrow to obtain base cells that will then be differentiated into macrophage and dendritic cells. The pure cultures of immune cells were then infected with the BCG and the infection rate was determined. Seven days after recombinant BCG infection, the dendritic and macrophage cells appeared to have engulfed and lysed the bacterial culture completely, the fluorescent gene having only been visible within the first three days of infection under a fluorescent microscope. This proved that after a short amount of time, the immune system cells were able to engulf and breakdown the subcellular components of the bacteria.

Erin Pickard, Hi-Plains High School.
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Author:Pickard, Erin
Publication:Journal of the Colorado-Wyoming Academy of Science
Article Type:Brief Article
Geographic Code:1USA
Date:Apr 1, 2002
Words:163
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