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Morphology of the spermatheca of Triatoma lecticularia (Hemiptera: Reduviidae) (Stal, 1859)/Morfologia da espermateca de Triatoma lecticularia (Hemiptera: Reduviidae) (Stal, 1859).

1. Introduction

The Triatominae (Hemiptera: Reduviidae) are obligate hematophagous insects feeding on different hosts. Many representatives of Triatominae are potential vectors of Trypanosoma cruzi (Chagas, 1909), the etiological agent of Chagas's disease (Lent and Wygodzinsky, 1979).

Triatoma lecticularia (Hemiptera: Reduviidae) (Stal, 1859) is a forest dwelling species found in the United States and Mexico (Lent and Wygodzinsky, 1979; Jurberg and Costa 1989; Silva et al., 1993; Galvao et al., 2003) and is generally found infected with T. cruzi, being important for the maintenance of the wild cycle of this pathogen at North America (Silva et al., 1993; Bern et al., 2011).

The reproductive female tract of Hemiptera is formed by ovaries, lateral oviducts, a common oviduct, accessory glands and spermatheca (Jahnke et al., 2006; Chiang et al., 2012; Chapman, 2013). During mating the spermatozoa are transferred together with the secretion of the male accessory glands to the spermatheca (Chapman, 2013). The spermatheca is an organ of the female reproductive tract, responsible for storing and maintaining the viability of spermatozoa from mating until oocyte fecundation, reducing or eliminating the need for multiple matings and, consequently, the probability of the female being preyed upon (Thornhill and Alcock 1983; Souza et al., 2008, 2016).

In the spermatheca, the spermatozoa are protected from physical damage as well as from contact with hemolymph content and free radicals (Collins et al., 2004; Al-Lawati et al., 2009; King et al., 2011; Pascini and Martins, 2017). Generally, the spermatozoa are stored in a spermathecal bulb that is associated with a muscular duct, which controls spermatozoa release. The reservoir is lined by a layer epithelial cells in bees (Dallai, 1975; Martins and Serrao, 2002; Souza et al., 2008) or multiple layers with different cell types in Hemiptera and Diptera (Souza et al., 2016; Pascini and Martins, 2017). The reservoir epithelium seems to contribute to the transportation of the hemolymph molecules to the spermathecal lumen (Gobin et al., 2006; Martins et al., 2008). In some insects, there is a spermathecal gland containing a secretion rich in glycogen, glycoproteins, phospholipids and mucopolysaccharides (Bhatnagar and Musgrave, 1971; Wolfner, 2011; Pascini and Martins, 2017), which is released in the reservoir lumen to contribute to the maintenance of the spermatozoa viability.

The spermatheca is present in all orders of insect and generally, there is one spermatheca per individual, although in Rhodnius prolixus (Hemiptera, Reduviidae) and representatives of Culicidae and Caliphoridae (Diptera) have been reported multiple spermathecae with different morphologies (Pascini and Martins, 2017).

The control of the spermatozoa release from the spermatheca during oocytes fecundation is carried out by the muscular duct (King et al., 2011; Chapman, 2013), which can also show morphological variation according to species (Pendergrast, 1957; Kocorek and Danielczok-Demska, 2002).

Considering the importance of the spermatheca in the reproductive biology of insects, studies that provide morphological and physiological data regarding this organ are important for future research into population management of insects that are disease vectors. This study described the histology and histochemistry of the spermatheca of T. lecticularia, an insect directly related to the maintenance of the forest cycle of T. cruzi.

2. Material and Methods

Mated Triatoma lecticularia females in oviposition were obtained from colonies in the Insectary of the Parasitology Department at the Universidade Federal do Triangulo Mineiro (UFTM), Uberaba, Minas Gerais, Brazil (19[degrees]44'21" S, 47[degrees]57'26" W). The insects were maintained in acrylic cylindrical vials containing a cardboard strip with accordion folds to increase surface area, providing space and permitting access for food. The vials were sealed with fine cottonwool balls to allow the blood meal of the insects which was realized every seven days, using chickens (Mendes, 2014).

2.1. Histology and histochemistry

Six mated T. lecticularia females were cryo-anesthetized at 0 [degrees]C and dissected in presence of 125 mM NaCl. The spermatheca were transferred to Zamboni fixative solution (Stefanini et al., 1967) for four hours, dehydrated in a graded ethanol series (70, 80, 90 and 95%), and embedded in historesin (Leica). Slices 3 [micro]m thick were stained with toluidine blue and analyzed under a light microscope.

Some sections were submitted for histochemical testing with mercury bromophenol to detect total proteins and Periodic Acid-Schiff (P.A.S) to detect neutral polysaccharides and glycoconjugates (Bancroft and Gamble, 2008).

3. Results

The female reproductive tract of T. lecticularia is formed by a pair of ovaries connected to lateral oviducts, a common oviduct and a pair of spermatheca (Figure 1).

Spermathecae are irregular shaped tubes with c.a. 3 mm width connected at the common oviduct by a narrow duct (Figure 2A). Glands associated with the spermatheca were no found in the T. lecticularia reproductive tract.

The lumen of the spermathecal reservoir was lined by a single layered columnar epithelium (Figure 2A, B) with the nucleus placed in the center of the cell with a predominance of decondensed chromatin (Figure 2B). The perinuclear and apical regions of the columnar cells were strongly basophil in comparison with the basal cell region.

The columnar epithelial cells were weakly positive for the P.A.S test, with homogenous distribution of glycoconjugates in whole cytoplasm (Figure 2C). The secretion in the spermathecal lumen was positive for the P.A.S (Figure 2C). The mercury bromophenol histochemical test showed strong reaction in the apical cell region (Figure 2D) as well as the secretion of the spermathecal lumen.

The connection of the spermathecal reservoir to the common oviduct is made via a short muscular duct with circular muscles (Figure 2E, F).

4. Discussion

The presence of the two tubular spermathecae without associated glands in T. lecticularia is similar to what has been reported in other Triatominae (Davey, 1958; Chiang et al., 2012; Nascimento, 2015), except in Triatoma infestans which has oval espermatheca (Nascimento, 2015).

In T. lecticularia, the spermatheca are sinuous with the reservoir connected to the common oviduct with a narrower duct in comparison with the duct portion near to the reservoir, as found in the triatomines Panstrongylus megistus, P. lignarius and T. tibiamaculata (Nascimento, 2015).

The spermatheca of T. lecticularia has a single layer of columnar cells lining a lumen that stores the spermatozoa. These cells are basophils in the apical region, indicating that they can assume a secretory function, releasing molecules into the spermathecal lumen contributing to spermatozoa maintenance.

To reinforce the secretory role of the spermathecal epithelium of T. lecticularia, the histochemical tests show that both the cell cytoplasm and the lumen content are positive for the P.A.S test, suggesting that the epithelial cells release polysaccharides and/or glycoconjugates to the spermathecal lumen or transport them from the hemolymph. The transportation of molecules from the hemolymph to the spermathecal lumen has been reported in Hymenoptera, due to the basal region of the epithelial cells of the reservoir rich in invaginations associated with mitochondria, which characterizes the active transportation of molecules (Dallai, 1975; Wheeler and Krutzsch, 1994; Martins et al., 2008; Pascini and Martins, 2017).

The accumulation of total proteins in the apical cell cytoplasm of the T. lecticularia spermatheca suggests that the proteins synthesized by these cells may be released into the lumen of the organ. According to Souza et al. (2008), the epithelium of the spermatheca of stingless bee Melipona quadrifasciata anthidioides (Hymenoptera, Apidae) has a single layered epithelium and apical basophil indicating a secretory function. However, M. quadrifasciata anthidioides and other Hymenoptera have a pair of glands for the spermatheca that take on the main secretory role (Dallai, 1975; Martins and Serrao, 2002; Gobin et al., 2006; Souza et al., 2008; Gotoh et al., 2009), structures absent in T. lecticularia.

In Heteroptera, the spermatheca has morphology distinct from that found in Triatoma spp. (Kocorek and Danielczok-Demska, 2002; Stacconi and Romani, 2011; Candan et al., 2014; Souza et al., 2016), with only one spermatheca. In these species, the reservoir shows three types of cells: i) class III secretory cells, characterized by the presence of intracellular canaliculi, ii) cells that form ducts for the transportation of secretions to the lumen of the spermatheca and iii) epithelial cells associated with the cuticle (Noirot and Quennedey, 1991; Stacconi and Romani, 2011; Souza et al., 2016; Pascini and Martins 2017).

A significant feature of the spermatheca of insects is the presence of the cuticle, due to its ectodermal origin (Chapman, 2013; Pascini and Martins, 2017). However, the cuticle was not observed in the spermatheca of T. lecticularia. The spermathecal epithelium in T. lecticularia assumes the secretory function and the absence of the cuticle indicates that the secretion is quickly transported into the lumen.

In conclusion, the spermatheca of T. lecticularia is similar to the spermathecae of other Reduviidae. In addition, the histological data reveal the importance of reservoir epithelium for the spermatozoa maintenance stored in this organ.

http://dx.doi.org/10.1590/1519-6984.180932

Acknowledgements

This research was supported by Minas Gerais State Research Agency (FAPEMIG).

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M. F. Monteiro (a), L. C. O. Lisboa (a), T. M. Carvalho-Costa (b), J. C. Nevoa (b), C. J. F. Oliveira (b), J. E. Serrao (c) andE. A. Souz (a)*

(a) Instituto de Ciencias Biologicas e da Saude, Universidade Federal de Vicosa--UFV, Campus Rio Paranaiba, Rodovia MG 230, Km 7, CEP 38810-000, Rio Paranaiba, MG, Brasil

(b) Laboratorio de Imunologia, Universidade Federal do Triangulo Mineiro--UFTM, Campus I, Praca Manoel Terra, 330, 1 Piso, N.Sa. da Abadia, CEP 38025-015, Uberaba, MG, Brasil

(c)Departamento de Biologia Geral, Universidade Federal de Vicosa--UFV, Avenida Peter Henry Rolfs, s/no, Campus Universitario, CEP 36570-000, Vicosa, MG, Brasil

*e-mail: edmilson.souza@ufv.br

Received: June 2, 2017--Accepted: August 22, 2017--Distributed: February 28, 2019 (With 2 figures)

Caption: Figure 1. Female reproductive tract of Triatoma lecticularia showing ovaries (OV), lateral oviducts (LO), common oviduct (CO) and two spermathecae (Sp). Bar: 3mm.

Caption: Figure 2. Histology section of the spermathecae of Triatoma lecticularia. A: General view of the spermatheca showing its elongated shape and the single layered epithelium (Ep) lining the lumen (L). Bar: 200[micro]m; B: Columnar epithelial cells (Ep) with basophil cytoplasm (*) and nucleus with predominate decondensed chromatin. Note lumen (L) filled with spermathozoa. Bar: 20[micro]m; C: Spermatheca submitted to the P.A.S test with positive reaction into the lumen (L) and epithelial cells (Ep) with homogenously positive cytoplasm. Bar: 20[micro]m; D: Spermatheca submitted to the mercury bromophenol test for proteins showing positive reaction into the lumen (L) and epithelium (Ep). Bar: 20[micro]m; E: Muscular duct (md) opening in the common oviduct (Co). Bar: 200[micro]m; F: Detail of the muscle layers (m) of the spermathecal duct. Bar: 50 [micro]m. n: nucleus.
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Title Annotation:Original Article
Author:Monteiro, M.F.; Lisboa, L.C.O.; Carvalho-Costa, T.M.; Nevoa, J.C.; Oliveira, C.J.F.; Serrao, J.E.; S
Publication:Brazilian Journal of Biology
Date:Feb 1, 2019
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