Molecular characterization of an invasive novel cydippid ctenophore.
MOLECULAR CHARACTERIZATION OF AN INVASIVE NOVEL CYDIPPID CTENOPHORE. R MARIITA, C LESTER, A.R. HOSKINS, J BRITT, M. KLINKA, C.P. WOOD AND A. M66. CELLULAR AND MOLECULAR BIOSCIENCES & DEPARTMENT OF BIOLOGICAL SCIENCES, AUBURN UNIVERSITY, AUBURN, AL 36849.
An invasive ctenophore was observed in Mobile Bay and Mississippi Sound, AL in September 2012. Morphological analyses most closely matched Pleurobrachia globosa Moser 1903 (LesterASLO 2013). Here we present the first molecular phylogenetic analysis of this ctenophore using 18S and ITS DNA. Ctenophores collected by gentle plankton net tows were transported to the laboratory within 12 hours. Genomic DNA extraction was carried out as described (Tech Mol Sys Evol 243)). Nine primer pairs (Podar1400F/Podar5'28srR; DiezEukA/DiezEuKB; 23fPL/518R; 18SAF/18SBR; 18hF/18pR; 18hF/18R1779F; 18eF/18iR; 18eR/18RR and 18eF/18pRF) were used for touchdown PCR; products ranged from 750 to 2000 bp. Phylogenetic analyses were done using Sequencher. Multiple alignments were performed with ClustalW and phylogenetic trees were created with MEGA. Maximum Parsimony Analysis was used to infer evolutionary history. Bootstrap analysis was done using 1000 replicates with a site coverage cut-off of 95%. Saccharomyces cerevisiae (NCBI accession number J01353.1|YSCRGEA) was used as the outgroup and for rooting. Assembled contigs compared against Gen Bank using BLASTn showed that all hits were below the 97% sequence similarity threshold for Hormiphora, Pleurobrachia, Mnemiopsis, Bolinopsis and Lampocteis. Phylogenetic analyses suggest that this is a novel invasive cydippid that may have arisen out of P. globosa and may be ancestral to Hormiphora. Acknowledgements: Drs. K. Halanych, K. Kocot for primers; NSF MCB0348327 and Hatch 0217327, AU IGM Program/Office of the Provost (AGM).
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|Title Annotation:||Biological Sciences Paper Abstracts|
|Publication:||Journal of the Alabama Academy of Science|
|Date:||Apr 1, 2014|
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