Printer Friendly

MoBiTec Launches SpeedBlot (His) - A Strikingly Fast and Easy-To-Use Western Blot Stain for His-Tagged Proteins.

Gottingen, Germany, June 12, 2014 --(PR.com)-- Western Blot is widely used in research to separate and identify proteins. In this technique a mixture of proteins is separated based on molecular weight through gel electrophoresis. The content of the gel is then transferred to a membrane producing a band for each protein. The protein of interest is then detected by means of a specific antibody.

Poly-histidine tags are often used for affinity purification of poly-histidine-tagged recombinant proteins expressed in Escherichia coli, Bacillus megaterium, Bacillus subtilis, and other prokaryotic expression systems. A poly-histidine tag is an amino acid motif in proteins that consists of multiple histidine (His) residues, mostly located at the N- or C-terminus of the protein. In order to facilitate the detection of successfully expressed his-tagged proteins the usage of anti-poly-histidine antibodies is a prevalent technique.

However, the common protocol for the antibody-based detection of the protein of interest is a laborious and time-consuming undertaking.

As time is money and to make life easier MoBiTec introduces SpeedBlot (His), a ready-to-use reagent for the detection of poly-histidine tagged proteins in blot assays:

SpeedBlot (His) is equally suitable for Western Blot assays and Dot or Slot Blot assays. The detection reagent is made of an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and N-terminal tags. The protein of interest is specifically stained in pink directly on the blot.

As Easy as Ponceau Red but as Specific as ECL Antibody!

Features

• Very fast protocol; results in 30-90 min

• Specific staining of His-tagged proteins

• Sensitive to sub-picomol amounts of protein

• Simple incubation, no buffer exchange, etc.

• Economic, no readout equipment required

• Visually detectable result

• Quantitative and permanent signal

• Works with C-/ N-terminal tags

The product is readily available. For further details please see: http://www.mobitec.com

Time is money! (Benjamin Franklin)

Contact Information:

MoBiTec GmbH

Arne Schulz

+49-551-70722-0

Contact via Email

www.mobitec.com

Read the full story here: http://www.pr.com/press-release/563574
COPYRIGHT 2014 PR.com
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2014 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Publication:PR.com (Press Releases)
Date:Jun 12, 2014
Words:335
Previous Article:MANteresting is Under New Ownership.
Next Article:Ambassador Kimon Valaskakis Appointed as Member of the Foundation for a World in Transition's International Advisory Board.
Topics:

Terms of use | Privacy policy | Copyright © 2021 Farlex, Inc. | Feedback | For webmasters |