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Methylotroph infections and chronic granulomatous disease.

Chronic granulomatous disease (CGD) is a primary immunodeficiency characterized by recurrent infections of the lung, skin, lymph nodes, and liver, as well as granulomatous inflammation affecting those organs and hollow viscera. The immunodeficiency results from deficiencies in any 1 of the 5 subunits forming the NADPH (nicotinamide adenine dinucleotide phosphate) oxidase 2 (Nox2)-based complex, which leads to impaired production of reactive oxygen species in phagocytes. Defects in the Nox2 (gp-[91.sup.phox]) enzymatic subunit (CYBB [cytochrome b-245, [beta] polypeptide]) are inherited in an X-linked manner, whereas defects in subunits [p47.sup.phox] (NCF1 [neutrophil cytosolic factor 1]), [p22.sup.phox] (CYBA [cytochrome b-245, a polypeptide]), [p67.sup.phox] (NCF2 [neutrophil cytosolic factor 2]), and [p40.sup.phox] (NCF4 [neutrophil cytosolic factor 4]) are inherited in an autosomal recessive manner (1,2).

CGD infections are often caused by a characteristic group of pathogens, including Staphylococcus aureus, Serratia marcescens, Burkholdheria cepacia complex, Nocardia spp., and Aspergillus spp. (1). However, new pathogens are emerging, and some reportedly are found almost exclusively in patients with CGD. Methylotrophs are bacteria that can use single-carbon organic compounds as their sole source of energy, the widespread availability of which makes these organisms versatile environmental inhabitants. However, they rarely cause disease in immunocompetent persons (3).

We previously reported 7 methylotroph infections in patients with CGD and here describe 5 more (Table). From these 12 infections, we have isolated the methylotrophs Granulibacter bethesdensis, Acidomonas methanolica, and Methylobacterium lusitanum. These infections were difficult to diagnose and required prolonged courses of antimicrobial drugs and sometimes surgery for complete resolution.

Patient 1

In 2008, a 1-year-old girl from Mexico who had [p67.sup.phox]-deficient CGD was examined for fever, weight loss, and enlarged cervical lymph nodes; she had been receiving ceftriaxone, clindamycin, itraconazole, and interferon-[gamma] for treatment of CGD. CGD was diagnosed when she was 6 months of age, at which time she had Penicillium sp. pneumonia and an abnormal dihydrorhodamine oxidation assay result. At 3 weeks of age, she had had a methicillin-sensitive S. aureus labial abscess, followed at 8 months of age by 3 episodes of pneumonia and an S. marcescens buttock abscess.

A computed tomographic (CT) scan performed at the time of admission showed cervical, mediastinal, and mesenteric lymphadenopathy and a right middle lung lobe infiltrate and hepatosplenomegaly. Excisional cervical lymph node and lung biopsy samples were processed for bacterial, nocardial, fungal, and mycobacterial cultures and staining. Direct Gram staining revealed moderate mononuclear cells but no organisms. After 11 days of incubation on chocolate agar at 37[degrees]C, the lung biopsy culture grew 1 pink colony. Species level identification conducted by full 16S rRNA gene sequencing ([approximately equal to] 1,500 bp) showed a 99.8% match to the M. lusitanum type strain (7). Etest (bioMerieux Diagnostics, Marcy l'Etoile, France) showed the following MICs (in gg/mL): amikacin (MIC = 4), cefepime (MIC = 8), ceftriaxone (MIC = 2), ciprofloxacin (MIC = 16), piperacillin-tazobactam (MIC = 2), imipenem (MIC = 2), meropenem (MIC [greater than or equal to] 32), trimethoprim/sulfamethoxazole (MIC >32), and aztreonam (MIC >256). Culture of the cervical lymph node biopsy sample grew S. marcescens.

After 7 months of treatment with ceftriaxone, clindamycin, and itraconazole, the patient completely recovered from the pneumonia and cervical lymphadenitis. She subsequently underwent successful hematopoietic stem cell transplant.

Patient 2

In 2011, a 19-year-old white man from Ohio, USA, who had X-linked CGD was examined for right neck swelling and tenderness (2 weeks' duration), a yellow ulcerated lesion on the right side of the hard palate, and an enlarged right tonsil with copious exudate. He had been receiving prophylactic trimethoprim-sulfamethoxazole and posaconazole. Erythrocyte sedimentation rate (ESR) was 28 mm/h, and C-reactive protein (CRP) concentration was 82 mg/L.

CGD had been diagnosed at birth on the basis of a positive family history. The patient had had hydrocephalus, catheter-associated fungal meningitis, and Aspergillus fumigatus pneumonia. When he was 15 years of age, CGD proctitis developed. Fourteen months before hospital admission, he had undergone right neck dissection for Rothia aeria infection, which was successfully treated with [beta]-lactams (8).

CT images showed new bulky lymphadenopathy in the right neck, involving all nodal planes, and increased thickening and asymmetry of the right oropharynx with hypoattenuation of the right palatine tonsils. Culture of the right tonsillar exudate and empirical treatment with meropenem were not helpful. Antimicrobial therapy was switched to ceftriaxone and high-dose penicillin for empirical coverage of G. bethesdensis and Actinomyces spp. Right neck dissection with tonsillectomy yielded Staphylococcus epidermidis, and full 16S rRNA gene sequencing ([approximately equal to] 1,500 bp) of 1 colony of a gram-negative bacillus showed a 99.8% match to the G. bethesdensis type strain. Nine weeks of vancomycin and ceftriaxone followed by 8 weeks of cefdinir, doxycycline, and rifampin led to complete resolution of the lymphadenitis.

Patient 3

In 2012, a 16-year-old boy from Portugal who had X-linked CGD was examined for fever, cervical lymphadenopathy, and elevated inflammatory markers. CT images showed a deep cervical abscess, from which nothing grew on culture but which completely resolved after 5 weeks of intravenous ceftriaxone, doxycycline, and ciprofloxacin and 6 weeks of oral amoxicillin/clavulanate, ciprofloxacin, and doxycycline, along with prophylactic itraconazole and interferon-[gamma].

Immediately after completion of that course of antimicrobial drugs, pneumonia with pleural effusions developed. Results of all cultures (blood, lymph node, broncheoalveolar lavage, and pleural fluid) were negative, but full 16S rRNA gene sequencing ([approximately equal to] 1,500 bp) of pleural fluid showed a >99% match to Cupriavidus spp. The patient received meropenem, ciprofloxacin, amikacin, doxycycline, teicoplanin, and voriconazole. Two weeks later, fever with splenomegaly, pancytopenia, low fibrinogen levels, and elevated ferritin and soluble CD25 levels were noted. Interferon-[gamma] prophylaxis was discontinued and the patient was administered dexamethasone and intravenous immunoglobulin, after which the presumed exuberant inflammatory response quickly resolved. CT images of the neck and lung were unremarkable, as were positron emission tomography images.

A month later, the boy was examined for fever, cough, and altered mental status; he required intubation and transfer to the intensive care unit. Magnetic resonance imaging revealed bilateral pneumonia and multiple intraparenchymal brain abscesses. Cerebrospinal fluid (CSF) was unremarkable. A lung biopsy sample, collected while the patient was receiving meropenem, ciprofloxacin, amikacin, doxycycline, teicoplanin, and voriconazole, was sterile. Voriconazole was switched to caspofungin and liposomal amphotericin B, and teicoplanin was switched to linezolid. The patient eventually recovered and was transferred out of the intensive care unit.

One month later, fever with focal neurologic deficits developed. CSF examination confirmed persistent pleocytosis with low glucose and elevated protein levels, and CT images indicated leptomeningitis. A 4-day culture of CSF on chocolate agar showed brownish colonies 1-2 mm in diameter. Full 16S rRNA gene sequencing ([approximately equal to] 1,500 bp) performed on the isolate from CSF showed a 99.7% match to the G. bethesdensis type strain. Etest showed the following MICs (in [micro]g/mL): tobramycin (MIC = 12), ceftriaxone (MIC >32), doxycycline (MIC = 24), and trimethoprim/ sulfamethoxazole (MIC = 0.25). Isoniazid, clarithromycin, and rifampin had already been added to the patient's treatment regimen. Despite the above interventions, the patient died of obstructive hydrocephalus and multiorgan failure.

Patient 4

In 2013, a 9-year-old multiracial boy from Iowa, USA, who had X-linked CGD was examined for a 1-day history of fever, fatigue, decreased appetite, headache, and neck pain. He had been receiving oral trimethoprim/sulfamethoxazole, voriconazole and interferon-[gamma] for CGD prophylaxis. When he was 1 month of age, he had had disseminated Candida lusitaniae infection with retropharyngeal, parapharyngeal, hepatic, and splenic abscesses. At 6 months of age, he was hospitalized for a progressively enlarging left posterior neck mass. A lymph node biopsy sample showed necrotizing granulomata and rare yeast forms suggestive of Histoplasma, but no specific organism was identified. He also had recurrent otitis media, tonsillitis, and aphthous stomatitis. Two months before the visit reported here, he had had Aspergillus versicolor pneumonia complicated by granulomatous appendicitis.

At the time of this hospital admission, he had 2 enlarged right anterior cervical nodes, which were soft, mobile, and not tender. ESR was 41 mm/h, and CRP concentration was 76 mg/L. CT images showed a 23 x 9 x 18-mm abscess adjacent to the right sternocleidomastoid muscle, extensive left supraclavicular lymphadenopathy, and left-sided pneumonia and pleural effusion.

Excisional biopsy of the right cervical lymph nodes yielded pus but no organisms. After 3 days of culture on chocolate agar, [approximately equal to] 20 tan colonies of an aerobic gram-negative bacillus were seen. The organism was oxidase-positive, catalase-positive, and indole-negative. After 6 days, abundant growth of a morphologically identical organism was seen on potato dextrose agar without antimicrobial agent and on Mycosel agar with chloramphenicol and cycloheximide but not on brain heart infusion agar with chloramphenicol and gentamicin (all media from Remel, Lenexa, KS, USA). Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (Biotyper system version 3.1; Bruker Daltonics Inc., Billerica, MA, USA) from directly smeared colonies with and without formic acid overlay (9) yielded no identification, and growth was insufficient for biochemical identification or susceptibility testing. Species-level identification conducted by 16S rRNA gene sequencing (ABI MicroSeq 500 kit; Thermo Fisher Scientific, Grand Island, NY, USA, and the IDNS SmartGene system, version 3.6.10; SmartGene Inc., Raleigh, NC, USA) was interpreted as A. methanolica (100% identity >388 bp with type strain CGDAM1) (7). No other pathogens were grown or amplified from any specimen.

The patient initially received piperacillin/tazobactam and vancomycin; liposomal amphotericin B was administered in view of his recent A. versicolor pneumonia. After 4 days, the piperacillin/tazobactam was switched to meropenem and ciprofloxacin was added. Levels of inflammatory markers eventually returned to reference values, and the lymphadenopathy improved after 5 weeks of intravenous meropenem and intravenous and oral ciprofloxacin. The patient was discharged with ciprofloxacin, voriconazole for A. versicolor pneumonia, prophylactic trimethoprim/sulfamethoxazole, and interferon-[gamma]. He subsequently underwent successful transplant of matched unrelated hematopoietic stem cells.

Patient 5

In 2014, a 36-year-old white man from Georgia, USA, who had X-linked CGD was hospitalized with cervical and abdominal lymphadenopathy; he had 1-year history of fever, malaise, and weight loss. X-linked CGD had been diagnosed when the patient was 6 months of age, when he had had hepatosplenomegaly, cervical lymphadenitis, and recurrent S. aureus infections. Aspergillus spp. pneumonia developed when he was 2 years of age, a liver abscess required incision and drainage when he was 4 years of age, and multiple abdominal abscesses required surgery when he was 6 years of age. Prophylactic trimethoprim/sulfamethoxazole for CGD had been effective until the hospitalization reported here.

A year before admission, the patient had experienced sudden onset of fever, cough, and shortness of breath. Right-sided pleural effusion was treated with antimicrobial drugs and thoracentesis. Cough and dyspnea improved, but fever, fatigue, malaise, myalgia, and weight loss of >15 kg were refractory to hydroxychloroquine and interferon-[gamma]. At 2 months before admission, he had had cervical and abdominal lymphadenopathy with ascites. Peritoneal fluid examination, esophagogastroduodenoscopy, and colonoscopy were not informative. Excisional biopsy of a right posterior cervical lymph node, tuberculin skin testing, and QuantiFERON-TB Gold (QIAGEN, Valencia, CA, USA) testing produced negative results. Ciprofloxacin and metronidazole did not abate symptoms and fever.

At admission, the patient had right supraclavicular and left axillary lymphadenopathy and hepatosplenomegaly. ESR was 88 mm/h, and CRP concentration was 131 mg/L. CT images showed lung scarring, splenomegaly, pericardial effusion, and multifocal adenopathy of the left axilla, mediastinum, celiac, periaortic, retroperitoneal, and mesenteric regions. Culture of excised axillary lymph node grew S. epidermidis, and G. bethesdensis (100% match to the G. bethesdensis type strain by full 16S rRNA gene sequencing, [approximately equal to] 1,500 bp). Etest of G. bethesdensis isolate showed the following MICs in pg/mL: tobramycin (MIC = 4), ceftriaxone (MIC = 32), doxycycline (MIC = 8), trimethoprim/ sulfamethoxazole (MIC = 2), and tigecycline (MIC = 16).

The patient was empirically administered vancomycin and ceftriaxone and was discharged with ceftriaxone, doxycycline, prophylactic trimethoprim/sulfamethoxazole, and itraconazole. His medication was eventually switched to cefdinir along with CGD prophylaxis. After 15 months, inflammatory markers and left neck and supraclavicular lymphadenopathy had improved but had not yet normalized.

Discussion

We have identified a total of 12 infections caused by 3 methylotroph bacteria in patients with CGD: 2 A. methanolica, 1 M. lusitanum, and 9 G. bethesdensis infections. Infections caused by A. methanolica and G. bethesdensis have been reported only for patients with CGD, whereas M. lusitanum in a patient without CGD undergoing chemotherapy for leukemia has been reported (10). These observations suggest that Nox2-based complex activity (superoxide production) is critical for protection against methylotroph infections. Consistent with this hypothesis, previous studies have demonstrated that G. bethesdensis persists in Nox2-based complex-deficient myeloid cells and is largely resistant to oxygen-independent microbicidal activity (11,12).

Methylotroph infections in CGD patients typically result in elevated inflammatory markers and lymphadenopathy, which may progress to necrotizing lymphadenitis with or without abscess formation. The clinical course may be protracted because of infection persistence, antimicrobial drug resistance, and relapse. Culturing these bacteria is difficult, requiring atypical media and prolonged incubation. These infections have not been fatal for patients in North and Central America, but G. bethesdensis infections in patients from Portugal and Spain (5) have caused fatal meningitis and bacteremia, respectively. The different clinical courses and outcomes for these patients compared with those in North and Central America suggest that the G. bethesdensis strain from Europe may be more virulent; animal studies are needed to explore this possibility. Furthermore, the strains isolated in Spain and Portugal showed only a 99.7% match with the 16S rRNA sequence of the type strain from North America, whereas most strains from North and Central America showed a 100% match with the type strain. Moreover, the strain from Europe displayed more in vitro resistance to antimicrobial agents and was resistant to colistin, most P-lactams, and quinolones (5). Although the value and accuracy of in vitro susceptibility testing for G. bethesdensis are unknown and may lack predictive value, the clinical and laboratory differences between G. bethesdensis strains from the United States and Europe may have substantial implications for therapy.

The facultative methylotrophs we report were difficult to culture; their correct identification required non-culture-based techniques, such as 16S rRNA gene sequencing, and a high index of suspicion. Application of 16S rRNA sequencing and molecular probes to target tissues may identify previously unrecognized bacteria, which may accompany and possibly facilitate methylotroph infections.

G. bethesdensis is a gram-negative, aerobic, oxidasenegative, and catalase positive bacillus. Culture is facilitated by specimen centrifugation and plating on buffered charcoal yeast extract agar or solid mycobacterial media; incubation takes up to 2 weeks (6). A. methanolica is a gram-negative, aerobic, acid-tolerant, catalase-positive, urease-positive, oxidase-positive, non-spore-forming, and nonmotile rod-shaped bacterium (13). It forms tan colonies in <5 days on chocolate agar and grows well on potato dextrose agar. M. lusitanum is a vacuolated gram-negative aerobic rod that is positive for indophenol oxidase, catalase, and urease and produces a pink pigment. It also reduces nitrate and assimilates malate (10). Methanol dehydrogenase serology is currently under investigation as a potential supportive diagnostic or prognostic tool for tracking methylotroph infections, particularly those caused by G. bethesdensis (14).

Management of methylotroph infections is often prolonged and may require combination antimicrobial drug therapy and surgery. Drug susceptibilities are difficult to determine and interpret. In vitro, G. bethesdensis is typically resistant to most penicillins, cephalosporins, carbapenems, and quinolones, but it is sometimes susceptible in vitro to ceftriaxone, aminoglycosides, doxycycline, and trimethoprim/sulfamethoxazole; combinations of these drugs have helped achieve initial resolution (6). Although antimicrobial drug susceptibilities for A. methanolica are not well defined, infections seemed to respond to combinations including meropenem, ciprofloxacin, gentamicin, doxycycline, and trimethoprim/sulfamethoxazole. M. lusitanum seems to be susceptible to aminoglycosides, cephalosporins, ciprofloxacin, piperacillin/tazobactam, and imipenem but not to meropenem, trimethoprim/sulfamethoxazole, or aztreonam. The role of surgery in treating methylotroph infections has not been defined, but its successful use in several cases is noteworthy.

In conclusion, methylotrophs are environmental organisms that can cause necrotizing infections in patients with CGD. Infectious prodromes and clinical courses may be prolonged. Diagnosis requires a high index of suspicion so that appropriate culture conditions and culture-independent techniques can be established for diagnosis. The difficulty of growing methylotrophs from infected lesions gives pause for the use of the label "sterile inflammation" with regard to CGD patients. Methylotrophs should be aggressively sought as the cause of chronic necrotizing infections in patients with CGD.

DOI: http://dx.doi.org/10.3201/eid2203.151265

Author affiliations: National Institutes of Health, Bethesda, Maryland, USA (E.L. Falcone, C.S. Zerbe, A.M. Zelazny, S.M. Holland); University of Iowa Hospital and Clinics, Iowa City, Iowa, USA (J.R. Petts, M.B. Fasano, B. Ford, W.M. Nauseef); Veterans Administration Medical Center, Iowa City (W.M. Nauseef); Centro Hospitalar de Lisboa Central, Lisbon, Portugal (J.F. Neves); Instituto Nacional de Saude Dr. Ricardo Jorge, Lisbon (M.J. Simoes); Detroit Medical Center, Detroit, Michigan, USA (M.L. Tierce IV); University of Texas Southwestern Medical Center, Dallas, Texas, USA (M.T. de la Morena, D.E. Greenberg)

This work was supported by the Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA (grant no. ZO1-AI-00646-06).

Dr. Falcone is a clinical fellow and an Oxford-Cambridge-National Institutes of Health scholar in the Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, at the National Institutes of Health. Her primary research interests include host-pathogen interactions in patients with CGD and the role of the microbiome in CGD colitis.

References

(1.) Segal BH, Leto TL, Gallin JI, Malech HL, Holland SM. Genetic, biochemical, and clinical features of chronic granulomatous disease. Medicine (Baltimore). 2000;79, 170-200. http,//dx.doi.org/10.1097/00005792-200005000-00004

(2.) Matute JD, Arias AA, Wright NA, Wrobel I, Waterhouse CC, Li XJ, et al. A new genetic subgroup of chronic granulomatous disease with autosomal recessive mutations in [p40.sup.phox] and selective defects in neutrophil NADPH oxidase activity. Blood. 2009; 114, 3309-15. http,//dx.doi.org/10.1182/ blood-2009-07-231498

(3.) Anthony C. The biochemistry of methylotrophs. New York, Academic Press; 1982. p. 2-3.

(4.) Chase JM, Holland SM, Greenberg DE, Marshall-Batty K, Zelazny AM, Church JA. Acidomonas methanolica-associated necrotizing lymphadenitis in a patient with chronic granulomatous disease. J Clin Immunol. 2012; 32,1193-6. http,//dx.doi.org/ 10.1007/s10875-012-9728-6

(5.) Lopez FC, de Luna FF, Delgado MC, de la Rosa II, Valdezate S, Nieto JA, et al. Granulibacter bethesdensis isolated in a child patient with chronic granulomatous disease. J Infect. 2008; 57,275-7. http,//dx.doi.org/10.1016/j.jinf.2008.04.011

(6.) Greenberg DE, Shoffner AR, Zelazny AM, Fenster ME, Zarember KA, Stock F, et al. Recurrent Granulibacter bethesdensis infections and chronic granulomatous disease. Emerg Infect Dis. 2010;16,1341-8. http,//dx.doi.org/10.3201/eid1609.091800

(7.) Clinical and Laboratory Standards Institute. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved guideline. Document no. MM18-A. Wayne (PA): The Institute; 2008.

(8.) Falcone EL, Zelazny AM, Holland SM. Rothia aeria neck abscess in a patient with chronic granulomatous disease: case report and brief review of the literature. J Clin Immunol. 2012; 32, 1400-3. http,//dx.doi.org/10.1007/s10875-012-9726-8

(9.) Ford BA, Burnham CA. Optimization of routine identification of clinically relevant gram-negative bacteria by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry and the Bruker Biotyper. J Clin Microbiol. 2013; 51, 1412-20. http,//dx.doi.org/10.1128/JCM.01803-12

(10.) Lai CC, Cheng A, Liu WL, Tan CK, Huang YT, Chung KP, et al. Infections caused by unusual Methylobacterium species. J Clin Microbiol. 2011;49,3329-31. http,//dx.doi.org/10.1128/ JCM.01241-11

(11.) Chu J, Song HH, Zarember KA, Mills TA, Gallin JI. Persistence of the bacterial pathogen Granulibacter bethesdensis in chronic granulomatous disease monocytes and macrophages lacking a functional NADPH oxidase. J Immunol. 2013; 191, 3297-307. http,//dx.doi.org/10.4049/jimmunol.1300200

(12.) Zarember KA, Marshall-Batty KR, Cruz AR, Chu J, Fenster ME, Shoffner AR, et al. Innate immunity against Granulibacter bethesdensis: an emerging gram-negative bacterial pathogen. Infect Immun. 2012;80,975-81. http,//dx.doi.org/10.1128/IAI.05557-11

(13.) Yamashita S, Uchimura T, Komagata K. Emendation of the genus Acidomonas Urakami, Tamaoka, Suzuki and Komagata 1989. Int J Syst Evol Microbiol. 2004;54,865-70. http,//dx.doi.org/ 10.1099/ijs.0.02946-0

(14.) Greenberg DE, Shoffner AR, Marshall-Batty KR, Arora K, Zhao M, Martin R, et al. Serologic reactivity to the emerging pathogen Granulibacter bethesdensis. J Infect Dis. 2012; 206, 943-51. http,//dx.doi.org/10.1093/infdis/jis431

Address for correspondence: E. Liana Falcone, NIH-LCID/NIAID, Rm B3-4141, 9000 Rockville Pike Blvd 10/CRC, Bethesda, MD 20892, USA; email: emilia.falcone@nih.gov
Table. Summary of methylotroph infections in patients with
chronic granulomatous disease *

Patient no.,    Patient     CGD genetics    Clinical findings
reference      age, y/sex    ([dagger])

1                 1/F         Autosomal        Pneumonia,
(this study)                  recessive         cervical
                                (NCF2         lymphadenitis
                              c.304C>T;
                              p.R102X)

2                 19/M        X-linked         Necrotizing
(this study)                    (CYBB           cervical
                             intragenic       lymphadenitis
                              deletion)

3                 16/M        X-linked         Meningitis
(this study)                    (CYBB
                               13-exon
                              deletion)

4                 9/M         X-linked          Cervical
(this study)                 (CYBB point        abscess,
                              mutation;       lymphadenitis
                              exon 10)

5                 36/M        X-linked         Multifocal
(this study)                (CYBB c.1139      lymphadenitis
                            G>A; p.W380X)

6 (4)             10/M        X-linked         Necrotizing
                                (CYBB           cervical
                             p.Arg226X)       lymphadenitis

7 (5)             10/M        X-linked         Bacteremia

8 (6)             39/M        X-linked         Necrotizing
                                                cervical,
                                              mediastinal,
                                                axillary
                                              lymphadenitis

9 (6)             36/M        X-linked         Multifocal
                                               necrotizing
                                             lymphadenitis,
                                                 splenic
                                                lesions,
                                                 ascites

10 (6)            13/M        X-linked         Necrotizing
                                                thoracic
                                              lymphadenitis

11 (6)            17/M        X-linked         Necrotizing
                                              cervical and
                                               mediastinal
                                              lymphadenitis

12 (6)            37/M        X-linked         Necrotizing
                                             supraclavicular
                                             lymphadenitis,
                                               splenic and
                                              liver lesions

Patient no.,    Microbiological        Treatment        Outcome
reference          findings        ([double dagger])

1              Methylobacterium    CRO/CLI/ITZ, HSCT   Recovered
(this study)   lusitanum (lung),
                   Serratia
                  marcescens
                 (lymph node)

2                Granulibacter         VAN/CRO,        Recovered
(this study)     bethesdensis;        CFD/DOX/RIF
                Staphylococcus
                  epidermidis

3               G. bethesdensis      MEM/CIP/AMK/         Died
(this study)                         DOX/TEC/VCZ,
                                   MEM/CIP/AMK/DOX/
                                     CSP/LAMB/LZD/
                                      RIF/INH/CLR

4                 Acidomonas       TZP/VAN/LAMB/CIP,   Recovered
(this study)      methanolica      MEM/VAN/LAMB/CIP,
                                    CIP/VCZ/TMPSMX/
                                   IFN-[gamma], HSCT

5              G. bethesdensis;        VAN/CRO,          Still
(this study)    S. epidermidis      CRO/DOX/TMPSMX/    receiving
                                          ITZ          treatment
                                                       as of 2015

6 (4)           A. methanolica      TMPSMX/CRO/DOX,    Recovered
                                    TMPSMX/RFB/GEN

7 (5)           G. bethesdensis    TMPS MX/CAZ/MTZ/       Died
                                        LZD/VCZ

8 (6)           G. bethesdensis    MEM/DOX, CRO/DOX

9 (6)           G. bethesdensis     MEM/TMPSMX/ITZ,    Recovered
                                    MEM/TMPSMX/VCZ/
                                   TOB, CRO/TMPSMX/
                                     IFN-[gamma],
                                    CPD/DOX/TMPSMX/
                                     IFN-[gamma],
                                    splenectomy/TGC

10 (6)         G. bethesdensis,      MEM/VCZ, CRO/     Recovered
                S. epidermidis,      TOB/DOX/VCZ,
               Candida glabrata,    CRO, DOX, CRO,
                 Streptococcus            CFD
                  mitis group

11 (6)          G. bethesdensis     LVX, DOX, lymph    Recovered
                                     node excision

12 (6)          G. bethesdensis      CRO/GEN/VAN,      Recovered
                                    CRO/DOX/TMPSMX,
                                    CFD/DOX/TMPSMX,
                                      DOX/TMPSMX

* AMK, amikacin; CAZ, ceftazidime; CFD, cefdinir; CGD, chronic
granulomatous disease; CLI, clindamycin; CLR, clarithromycin;
CIP, ciprofloxacin; CPD, cefpodoxime; CRO, ceftriaxone; CSP,
caspofungin; CYBB, cytochrome b-245, [beta] polypeptide; DOX,
doxycycline; GEN, gentamicin; HSCT, hematopoietic stem cell
transplant; IFN-[gamma], interferon-[gamma]; INH, isoniazid; ITZ,
itraconazole; LAMB, liposomal amphotericin B; LVX, levofloxacin;
LZD, linezolid; MEM, meropenem; MTZ, metronidazole; NCF2,
neutrophil cytosolic factor 2; RFB, rifabutin; RIF, rifampin;
TEC, teicoplanin; TGC, tigecycline; TMPSMX,
trimethoprim/sulfamethoxazole; TOB, tobramycin; VAN, vancomycin;
VRZ, voriconazole.

([dagger]) lnformation in parentheses indicates, when known, the
mutation that led to CGD.

([double dagger]) Slashes separate drugs in the same regimen;
commas separate regimens.
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Title Annotation:SYNOPSIS
Author:Falcone, E. Liana; Petts, Jennifer R.; Fasano, Mary Beth; Ford, Bradley; Nauseef, William M.; Neves,
Publication:Emerging Infectious Diseases
Geographic Code:1U5MD
Date:Mar 1, 2016
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