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Methylarsonous acid transport by aquaglyceroporins.

Many mammals methylate trivalent inorganic arsenic in liver to species that are released into the bloodstream and excreted in urine and feces. This study addresses how methylated arsenicals pass through cell membranes. We have previously shown that aquaglyceroporin channels, including Escherichia coli GlpF, Saccharorayces cerevisiae Fps1p, AQP7, and AQP9 from rat and human, conduct trivalent inorganic arsenic [As(III)] as arsenic trioxide, the protonated form of arsenite. One of the initial products of As(III) methylation is methylarsonous acid [MAs(III)], which is considerably more toxic than inorganic As(III). In this study, we investigated the ability of GlpF, Fps1p, and AQP9 to facilitate movement of MAs(III) and found that rat aquaglyceroporin conducted MAs(III) at a higher rate than the yeast homologue. In addition, rat AQP9 facilitates MAs(III) at a higher rate than As(III). These results demonstrate that aquaglyceroporins differ both in selectivity for and in transport rates of trivalent arsenicals. In this study, the requirement of AQP9 residues Phe-64 and Arg-219 for MAs(III) movement was examined. A hydrophobic residue at position 64 is not required for MAs(III) transport, whereas an arginine at residue 219 may be required. This is similar to that found for As(III), suggesting that As(III) and MAs(III) use the same translocation pathway in AQP9. Identification of MAs(III) as an AQP9 substrate is an important step in understanding physiologic responses to arsenic in mammals, including humans. Key words: arsenic trioxide, AQP9, aquaglyceroporin, methylarsonous acid. doi:10.1289/ehp.8600 available via http://dx.doi.org/ [Online 2 December 2005]

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Trivalent inorganic arsenic [As(III)] is a known human carcinogen, with liver, skin and lung as target sites (Abernathy et al. 1999). In solution at physiologic pH, As(III) is primarily in the form of the undissociated acid arsenic trioxide [As[(OH).sub.3]] and not the oxyanion arsenite (Ramirez-Solis et al. 2004). The anhydrous form of arsenite trioxide ([As.sub.2][O.sub.3]) is also used clinically as a chemotherapeutic agent for the treatment of acute promyelocytic leukemia (Soignet et al. 1998). In tissues of some mammalian species, As(III) is methylated, forming trivalent and pentavalent products such as methylarsonous acid [MAs(III)], methylarsonic acid [MAs(V)], dimethylarsinous acid [DMAs(III)], dimethylarsonic acid [DMAs(V)], trimethylarsine oxide [TMAs(V)O] and trimethylarsine [TMAs(III)] (Cullen et al. 1984; Waters et al. 2004a). The liver is considered a major site of arsenic methylation, although other tissues also have the capacity to methylate arsenic (Vahter 2002). In humans, DMAs(III) and DMAs(V) appear to be the end products of this pathway (Thomas et al. 2001). MAs(III) and DMAs(III) are significantly more cytotoxic than As(III), perhaps because they are more potent inhibitors of critical enzymes (Thomas et al. 2001) or more efficient modulators of signal transduction pathways that regulate cellular metabolism and survival (Drobna et al. 2003; Walton et al. 2004). Thus, although the overall role of biomethylation in modulation of As(III) toxicity remains unclear, it is possible that MAs(III) and DMAs(III) significantly contribute to adverse effects associated with acute or chronic exposure to As(III).

A mammalian As(III) methyltransferase, Cyt19 or AS3MT, has been identified in rat liver (Lin et al. 2002; Walton et al. 2003). This enzyme catalyzes the sequential transfer of methyl groups from S-adenosylmethionine to trivalent arsenicals and the reduction of pentavalent methylated intermediates in the metabolic pathway for As(III) (Waters et al. 2004a, 2004b). The methylated arsenicals are released from liver into the bloodstream and end up in urine, skin, hair, and other tissues. For investigators to understand the mechanisms of arsenic toxicity and carcinogenesis as well as the ability of arsenic to serve as a chemotherapeutic agent, it is important to determine how methylated arsenicals are transported out of the liver and how they enter target tissues.

In this study, we show that aquaporin 9 (AQP9) conducts MAs(III). AQP9 is a member of the aquaglyceroporin family of channels that facilitates bidirectional movement of small neutral solutes such as glycerol and urea (Agre and Kozono 2003). Notably, the tissues with the highest expression of AQP9 include the liver (Abedin et al. 2002), an organ that plays a key role in the metabolism of arsenic. AQP9 is essential for the glycerol transport in the bile ducts and ductules of the liver. We previously identified the Escherichia coli aquaglyceroporin GlpF as a channel for As(III) and trivalent inorganic antimony [Sb(III)] (Meng et al. 2004; Sanders et al. 1997). In E. coli, uptake of metalloids produces toxicity. In contrast, in Sinorhizobium meliloti, downhill efflux of internally generated As(III) by another aquaglyceroporin, AqpS, confers resistance (Yang et al. 2005). Thus, aqualglyceroporins catalyze bidirectional movement of trivalent arsenicals. In Saccharomyces cerevisiae, the aquaglyceroporin Fps1p mediates uptake of As[(OH).sub.3], producing sensitivity. We have shown that rat AQP9, which has broadest solute permeability (Borgnia et al. 1999) among mammalian aquaglyceroporins, facilitates As[(OH).sub.3] transport when expressed in frog oocytes or S. cerevisiae (Liu et al. 2002). Thus, movement of arsenite appears to be a ubiquitous property of aquaglyceroporin channels from prokaryotes to eukaryotes.

In solution at neutral pH, the most likely form of MAs(III) is methanearsonous acid [C[H.sub.3]As[(OH).sub.2]], so it was reasonable to consider the possibility that MAs(III) uses the same translocation pathway as As[(OH).sub.3]. Here, we show that rat AQP9 conducts MAs(III). In contrast, yeast Fps1p did not exhibit significant MAs(III) conductivity, demonstrating that aquaglyceroporins have a wide variation in selectivity for arsenicals. We examined the roles of two specific residues in AQP9 in MAs(III) transport by site-directed mutagenesis. From the crystal structures of GlpF (Fu et al. 2000) and AQP1 (Sui et al. 2001), it has been proposed that the positive charge of GlpF residue Arg-195 and AQP1 residue Arg-206 serve as the filter that prevents movement of protons or other positive ions (Jensen et al. 2003; Tajkhorshid et al. 2002). In this study, we showed that substitution of AQP9 residue Arg-219 eliminates MAs(III) uptake, consistent with a requirement for an arginine residue for conduction of C[H.sub.3]As[(OH).sub.2]. The hydrophobic residue Phe-56 in AQP1 has been proposed to orientate the water molecule to form a hydrogen bond with Arg-195 (de Groot and Grubmuller 2001). The effect of substitution of the corresponding residue in AQP9, Phe-64, was investigated, and the results indicate that a hydrophobic residue is not required at that position. We propose that AQP9 is central to arsenic metabolism in liver, and aquaglyceroporins may play similar roles in other tissues. As(III) enters the hepatocyte down its concentration gradient through AQP9. Inside the cell, As(III) is methylated and reduced to MAs(III). Internally generated MAs(III) then flows out of the hepatocyte and into the bloodstream down its concentration gradient via AQP9.

Materials and Methods

Strains and plasmids. E. coli and S. cerevisiae strains and plasmids used in this study are described in Table 1. Two E. coli strains were used. The chromosomal arsRBCoperon, which confers low-level resistance to arsenite, had been deleted to create the arsenic-hypersensitive strain AW3110 (Carlin et al. 1995). Strain OSBR1 was derived from AW3110 by inactivation of the glpF gene. Disruption of glpF makes the cells arsenite resistant again because arsenite cannot get into the cells (Sanders et al. 1997). Similarly, two S. cerevisiae strains were used. In yeast strain MG102, the YCF1 and ACR3 genes encoding arsenite resistance transporters were disrupted, rending the cells sensitive to arsenite (Ghosh et al. 1999). Strain HD9 was derived from MG102 by disruption of the FPS1 gene (Liu et al. 2002), which makes the yeast cells resistant to arsenite because it is not taken up.

Chemicals and media. Methylarsine oxide provided by W.R. Cullen (University of British Columbia, Vancouver, Canada) was used as a precursor of MAs(III). Methylarsine oxide is hydrolyzed to MAs(III) in aqueous solution (Petrick 2001). Sodium arsenite was purchased from Sigma Chemical Co. (St. Louis, MO). S. cerevisiae strains were grown at 30[degrees]C in minimal synthetic dextrose (SD) medium (Adams et al. 1998) with 2% galactose and supplemented with the required auxotrophic requirements. E. coli cells were grown in Luria-Bertani (LB) medium (Sambrook et al. 1989) at 37[degrees]C.

DNA manipulations and site-directed mutagenesis. Plasmid pAQP9 carrying the rat AQP9gene (Liu et al. 2002) was purified using Qiagen miniprep spin column and used for site-directed mutagenesis (Stratagene, La Jolla, CA). Each mutation was verified by DNA sequencing of the entire gene. The mutants were transformed into yeast using a Geno easy-transform kit (Geno Technologies, St. Louis, MO). Oligonucleotides used were as follows: R219E forward, GGG ACT GAG GTC TTC AGC TGG GTT C; R219E reverse, GAA CCC AGC TGA AGA CCT CAG TCC C; R219H forward, GGG ACT GAG GTC ATG AGC TGG GTT C; R219H reverse, GAA CCC AGC TCA TGA CCT CAG TCC C. Construction of the R219A, R219K, F64A, F64W, and F64T mutants has been previously described (Liu et al. 2004). Expression of each mutant AQP9 protein was verified by sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting, as described previously (Liu et al. 2004).

Metal ion resistance assays. For E. coli, strains were grown overnight at 37[degrees]C in liquid LB medium with shaking, diluted into LB with the indicated concentrations of metalloid, and allowed to grow with shaking for an additional 8 hr. For S. cerevisiae, strains were grown overnight at 30[degrees]C in liquid SD medium with 2% galactose and the appropriate supplements. The cultures were diluted into fresh SD media to an optical density at 600 nm ([OD.sub.600nm]) of 0.2 in the presence of varying concentrations of the metalloid salts and incubated for an additional 20 hr, after which growth was estimated from [OD.sub.600nm]. For growth on solid media, yeast strains were grown overnight at 30[degrees]C in liquid SD medium with 2% galactose. Equivalent numbers of cells from each culture were spotted in 10-fold dilutions with the indicated concentrations of As(III) or MAs(III). Growth was observed after 3 days of incubation at 30[degrees]C.

Transport assays. In vivo metalloid uptake assays were performed as previously described (Liu et al. 2002). Briefly, either E. coli or yeast cells were grown to exponential phase, harvested, washed with transport buffer consisting of 75 mM HEPES, 0.15 M KCl, 1 mM Mg[Cl.sub.2], pH 7.3, and suspended to a density of 2 x [10.sup.8] cells/mL in the same buffer containing 0.1 M glucose, all at room temperature. The assay was initiated by addition of either 10 [micro]M MAs(III) or 10 [micro]M As(III), final concentration. Portions (0.1 mL) were withdrawn at intervals and filtered through nitrocellulose filters (0.5-[micro]m pore size; Whatman Inc., Florham Park, NJ). The filters were washed with 5 mL of transport buffer 3 times and dried. The membrane was treated in 70% nitrate acid at 70[degrees]C until dissolved, and HPLC-quality water added to bring each sample to 7 mL. The amount of arsenic was determined by inductively coupled plasma mass spectrometry (ICP-MS) (PerkinElmer, Norwalk, CT).

Results

MAs(III) uptake in E. coli. We have previously shown that the aquaglyceroporin GlpF facilitates uptake of As[(OH).sub.3] in E. coli and that deletion of GlpF results in loss of uptake (Meng et al. 2004). Here we examined the ability of GlpF to transport MAs(III). As(III) and MAs(III) sensitivity and uptake were compared in two E. coli strains. One was AW3110 ([DELTA]arsRBC), in which the chromosomal arsRBC operon, which confers resistance to both arsenate and arsenite, was deleted. Thus, this strain is hypersensitive to AS(III). The second strain was OSBR1 ([DELTA]arsRBC[DELTA]glpF), which was derived from AW3110 by disruption of the gene for GlpF. Because this lacks the uptake system for As(III), it is resistant to the metalloid. Both strains were sensitive to MAs(III) (Figure 1A) and transported MAs(III) similarly (Figure 1B). These data suggest that most MAs(III) is taken up by a system or systems other than GlpF, which accounts for the sensitivity of E. coli to this methylated species. GlpF participation in MAs(III) transport, if any, is negligible. In contrast, AW3110 takes up AS(III), whereas OSBR1 does not (Figure 1C). Because of the high rate of uptake of MAs(III), it is not possible to conclude whether GlpF conducts MAs(III).

[FIGURE 1 OMITTED]

MAs(III) uptake in S. cerevisiae by Fps1p and mammalian AQP9. The ability of the yeast aquaglyceroporin Fps1p to conduct MAs(III) was examined. In these assays, S. cerevisiae strains MG102 (acr3[DELTA] ycf1[DELTA]) and HD9 (acr3[DELTA] ycf1[DELTA] fps1[DELTA]) were used. MG102 is hypersensitive to AS(III) because both ACR3, which encodes a plasma membrane As(III) extrusion carrier, and YCF1, which encodes a homologue of the ATP binding cassette ATPase multidrug resistance-associated protein (ABC ATPase MRP) (Cole et al. 1994) and sequesters arsenite triglutathione [As(GS)3] in the vacuole, were deleted (Ghosh et al. 1999). Like its parent, HD9 is acr3[DELTA] ycf1[DELTA] but has become resistant to As(III) because it is also fps1[DELTA] and so does not take up As(III) (Liu et al. 2002). MG102 and HD9 exhibited similar resistance to MAs(III), as did HD9 expressing FPS1 from a plasmid (Figure 2A). However, when rat AQP9 was expressed from a plasmid in HD9, yeast cells became sensitive to MAs(III).

[FIGURE 2 OMITTED]

The resistance phenotype could be related to the ability of the cells to take up MAs(III). Cells of MG102, HD9, or HD9 [pFPS1.sub.took] up little MAs(III), but expression of rat AQP9 from a plasmid resulted in a high rate of uptake of MAs(III) in HD9 (Figure 2B). Because MG102 takes up considerably more As(III) than does HD9 (Liu et al. 2002), these results indicate that Fps1p facilitates uptake of As(III) but not MAs(III). In contrast, mammalian AQP9 takes up both inorganic and monomethylated trivalent arsenic. Strikingly, AQP9 conducts MAs(III) nearly 3 times faster than it does As(III) (Figure 3). These results indicate that AQP9 is more selective for the monomethylated form than for the inorganic form of trivalent arsenic, whereas Fps1p is highly specific for the inorganic form. The ability of AQP9 to conduct MAs(III) at a higher rate than As(III) is in good agreement with the results of recent studies that show considerably greater uptake and retention of MAs(III) than As(III) in cultured mammalian cells (Dopp et al. 2004; Drobna et al. 2005). Human AQP9 is 75% identical to and exhibits 86% overall similarity with the rat channel, and both transport As(III) with similar rates (Liu et al. 2004), so it is reasonable to expect that the human channel also conducts MAs(III). However, the human enzyme does not express well in yeast (Liu et al. 2004), so its ability to conduct MAs(III) was not tested here.

[FIGURE 3 OMITTED]

Structure--function relationships in AQP9 transport of MAs(III). We addressed the question of selectivity in aquaglyceroporins by mutagenesis of residues predicted to be involved in this process, including Arg-219, Trp-48, and Phe-200 of AQP9. To examine the requirement for a positive charge for MAs(III) uptake, Arg-219 was changed to alanine, lysine, glutamate, and histidine. Similar amounts of wild-type and mutant AQP9s were detected in the yeast membranes by immunoblotting (data not shown). HD9, which lacks the FPS1 gene, is resistant to MAs(III) (Figure 4A) because it cannot take up the methylated metalloid (Figure 4B). Expression of wild-type mouse AQP9 renders cells of HD9 sensitive to MAs(III) (Figure 4A) because the cells take up MAs(III) (Figure 4B). Cells expressing any of the mutants remained resistant to MAs(III) (Figure 4A) and were unable to take up the organic arsenical (Figure 4B). Although loss of function in mutants could result from protein misfolding, these results are consistent with an arginine residue being required at position 219 for uptake of MAs(III) and suggest that MAs(III) shares the same channel pathway as As(III) and glycerol.

[FIGURE 4 OMITTED]

We have previously shown that F64A, F64T, and F64W AQP9s are expressed in yeast and conduct As(III) and glycerol as well as wild-type AQP9 (Liu et al. 2004). Each mutant was transformed into yeast strain HD9, and both MAs(III) sensitivity and uptake was compared with HD9 transformed with wild-type AQP9. Again, with only vector plasmid pYES3, HD9 was resistant to MAs(III) and did not accumulate the metalloid, whereas wild-type AQP9 facilitated MAs(III) uptake and hence rendered the cells sensitive (Figure 5). Yeast cells expressing each of the Phe-64 mutants was as sensitive to MAs(III) as cells expressing wild-type AQP9 (Figure 5A). Cells expressing each mutant accumulated MAs(III), although at a somewhat lower rate than wild type (Figure 5B). Although a hydrophobic channel lining had been proposed to be required for AQP function, these results demonstrate that a hydrophobic residue at position 64 is not required for MAs(III) transport.

[FIGURE 5 OMITTED]

Discussion

Aquaglyceroporins have been shown to facilitate uptake of As(III), including E. coli GlpF (Sanders et al. 1997), S. cerevisiae Fps1p (Liu et al. 2002; Wysocki et al. 2001), mouse AQP7 (Liu et al. 2002), and AQP9 from rat (Liu et al. 2002) and humans (Liu et al. 2004). Once inside human cells, As(III) is methylated to a variety of species, of which the monomethylated form represents a significant fraction of total arsenic found in most tissues (Hughes et al. 2003; Kenyon et al. 2005). The primary site of methylation is liver, but other organs such as kidney or testes may also methylate As(III) (Healy et al. 1998). The final fate of the methylated species is excretion, both in urine and in feces. How these compounds find their way from liver to other tissues such as blood, kidney, or cecum is not certain, and the routes of efflux of methylated arsenical from hepatocytes and uptake into other cell types are unknown. In solution at physiologic pH, inorganic trivalent arsenic is As[(OH).sub.3] (Ramirez-Solis et al. 2004). We would predict that, in solution, the monomethylated species would be C[H.sub.3]As[(OH).sub.2], molecularly similar to but less polar than As[(OH).sub.3]. Because As[(OH).sub.3] is a substrate of aquaglyceroporins, we considered the possibility that those channels would also conduct C[H.sub.3]As[(OH).sub.2]. The answer was unexpected in that the aquaglyceroporins exhibit selectivity in their ability to facilitate transport of trivalent arsenicals. AQP9 transports both As[(OH).sub.3] and C[H.sub.3]As[(OH).sub.2] but is more selective for the latter. In contrast, neither GlpF nor Fps1p exhibited any ability to facilitate C[H.sub.3]As[(OH).sub.2] transport and yet are able to transport As(III) as well as they do glycerol. It is interesting to note that E. coli possesses an endogenous uptake system for MAs(III), whereas yeast does not.

What governs the selectivity of the aquaglyceroporins for different trivalent arsenicals? In particular, what allows AQP9 to conduct MAs(III) when at least two other aquaglyceroporins cannot? The structures of GlpF and the strict water channel AQP1 have been determined at atomic resolution. The spacing at the narrowest region of the pore (the aromatic/arginine ring) is significantly wider in GlpF [3.4 A (Nollert et al. 2001)] compared with AQP1 [2.8 [Angstrom] in AQP1 (Sui et al. 2001)], which accounts for the broader selectivity of the aquaglyceroporin. As[(OH).sub.3] is a symmetrical molecular with an O-O distance of approximately 3 [Angstrom], just small enough to fit through the GlpF channel. C[H.sub.3]As[(OH).sub.2] is asymmetric compared with As[(OH).sub.3], and the As--C bond is longer than the As--O bond, which may account for the selectivity of GlpF. Although its channel diameter is not known, AQP9 transports a much wider range of substrates than does GlpF, suggesting that larger molecules may be able to traverse its channel.

Little is known about the relationship of individual residues to the function of AQP9. In members of the AQP superfamily, there is often a conserved arginine after the second asparagine-proline-alanine motif (Borgnia et al. 1999). From analysis of the crystal structures of AQP1 and GlpF, it has been proposed that the positive charge of this arginine residue forms an electrostatic triangle with two negatively charged residues to polarize the substrate molecules and to provide a filter that prevents charged molecules from entering the channel. We have previously shown that a positive charge is required on the corresponding residue in AQP9 (Arg-219) for both As(III) and glycerol uptake (Liu et al. 2004). Our results suggest that an arginine residue is preferred at position 219. In the structure of GlpF, the aromatic rings of Trp-48 and Phe-200 are perpendicular to each other and form a hydrophobic corner that has been proposed to serve as a selectivity filter (Lee et al. 2004). Corresponding to Trp-48 in AQP1 is the hydrophobic residue Phe-56, which has been proposed to orientate the water molecule to form a hydrogen bond with Arg-195. Because mutagenesis of the corresponding hydrophobic residue in AQP9, Phe-64, was not deleterious, a hydrophobic residue at this position is not required.

AQP9 is highly expressed in liver (Abedin et al. 2002), where it plays an essential role in glycerol and urea transport (Carbrey et al. 2003). Because liver is also a key site for the metabolism of arsenic, we propose a model in which AQP9 catalyzes a key step in uptake of As(III) and efflux of MAs(III) (Figure 6). As(III) is taken up from the bloodstream by hepatocytes via AQP9. Inside the hepatocyte, it is methylated and reduced to MAs(III), which has a number of possible fates. It can be further methylated or glutathionylated. In mammals, both As[(GS).sub.3] and methylarsenite diglutathione [MAs[(GS).sub.2]] are pumped into bile by multidrug resistance-associated protein 2 (MRP2) or homologues (Kala et al. 2000). Internally generated MAs(III) can also flow out of the cell down its concentration into the bloodstream. AQP9 expression in rat liver was induced up to 20-fold by fasting (Carbrey et al. 2003), suggesting that uptake of As(III) and redistribution of MAs(III) may be nutritionally responsive. Once in the bloodstream, MAs(III) can be redistributed into other tissues, including blood cells and kidney, where it is excreted. We predict that there are pathways for uptake and excretion of di- and trimethylated species (both tri- and pentavalent) as well, and it is important to identify their transport pathways. Identification and characterization of each transport system forms the basis for our future studies.

[FIGURE 6 OMITTED]

Received 20 August 2005; accepted 1 December 2005.

REFERENCES

Abedin MJ, Cresser MS, Meharg AA, Feldmann J, Cotter-Howells J. 2002. Arsenic accumulation and metabolism in rice (Oryza sativa L). Environ Sci Technol 36(5):962-968.

Abernathy CO, Liu YP, Longfellow D, Aposhian HV, Beck B, Fowler B, et al. 1999. Arsenic: health effects, mechanisms of actions, and research issues. Environ Health Perspect 107:593-507.

Adams A, Gottschiing DE, Kaiser C, Stearns T. 1998. Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual. Cold Spring Harbor, NY:Cold Spring Harbor Laboratory Press.

Agre P, Kozono D. 2003. Aquaporin water channels: molecular mechanisms for human diseases. FEBS Lett 555(1):72-78.

Borgnia M, Nielsen S, Engel A, Agre P. 1999. Cellular and molecular biology of the aquaporin water channels. Annu Rev Biochem 68:425-458.

Carbrey JM, Gorelick-Feldman DA, Kozono D, Praeterius J, Nielsen S, Agre P. 2003. Aquaglyceroporin AQP9: solute permeation and metabolic control of expression in liver. Proc Natl Acad Sci USA 100(5):2945-2950.

Carlin A, Shi W, Dey S, Rosen BP. 1995. The ars operon of Escheriohia coli confers arsenical and antimonial resistance. J Bacteriol 177(4):981-986.

Cole SP, Sparks KE, Fraser K, Lee DW, Grant CE, Wilson GM, et al. 1994. Pharmacological characterization of multidrug resistant MRP-transfected human tumor cells. Cancer Res 54(22): 5902-5910.

Cullen WH, McBride BC, Pickett AW, Reglinski J. 1984. The wood preservative chromated copper arsenate is a substrate for trimethylarsine biosynthesis. Appl Environ Microbiol 47(2): 443-444.

de Greet BL Grubmuller H. 2001. Water permeation across biological membranes: mechanism and dynamics of aquaperin-1 and GlpF. Science 294(5550):2353-2357.

Dupp E, Hartmann LM, Florea AM, von Hecklinghausen U, Pieper R, Shokouhi B, et al. 2004. Uptake of inorganic and organic derivatives of arsenic associated with induced cytotoxic and genotoxic effects in Chinese hamster ovary (CHO) cells. Toxicol Appl Pharmacol 201(2):156-165.

Drobna Z, Jaspers I, Thomas D J, Styblo M. 2003. Differential activation of AP-1 in human bladder epithelial cells by inorganic and methylated arsenicals. FASEB J 17(1):67-69.

Drobna Z, Waters SB, Devesa V, Harmon AW, Thomas DJ, Styblo M. 2005. Metabolism and toxicity of arsenic in human urothelial cells expressing rat arsenic (+3 oxidation state)-methyitransferase. Toxicol Appl Pharmacol 207(2):147-159.

Fu D, Libson A, Miercke LJ, Weitzman C, Notlert P, Krucinski J, et al. 2000. Structure of a glycerol-conducting channel and the basis for its selectivity. Science 290(5491):481-486.

Ghosh M, Shen J, Rosen BP. 1999. Pathways of As(Ill) detoxification in Saccharomyces cerevisiae. Proc Natl Acad Sci USA 96:5001-5006.

Healy SM, Casarez EA, Ayala-Fierro F, Aposhian H. 1998. Enzymatic methylation of arsenic compounds. V. Arsenite methyltransferase activity in tissues of mice. Toxicol Appl Pharmacol 148(1):65-70.

Hughes MF, Kenyon EM, Edwards BC, Mitchell CT, Raze LM, Thomas DJ. 2003. Accumulation and metabolism of arsenic in mice after repeated oral administration of arsenate. Toxicol Appl Pharmacel 191(3):202-210.

Jensen MO, Tajkhorshid E, Schulten K. 2003. Electrostatic tuning of permeation and selectivity in aquaporin water channels. Biophys J 85(5):2884-2899.

Kala SV, Neely MW, Kala G, Prater CI, Atwood DW, Rice JS, et al. 2000. The MRP2/cMOAT transporter and arsenic-glutathione complex formation are required for biliary excretion of arsenic. J Biol Chem 275(43):33404-33408.

Kenyon EM, Del Raze LM, Hughes MF. 2005. Tissue distribution and urinary excretion of inorganic arsenic and its methylated metabolites in mice following acute oral administration of arsenate. Toxicol Sci 85(1):468-475.

Lee JK, Khademi S, Harries W, Savage D, Miercke L Stroud RM 2004. Water and glycerol permeation through the glycerol channel GlpF and the aquaporin family. J Synchrotron Radiat 11(pt 1):86-88.

Lin S, Shi Q, Nix FB, Styblo M, Beck MA, Herbin-Davis KM, et al. 2002. A novel S-adenosyI-L-methionine:arsenic(III) methyltransferase from rat liver cytosol, d Biol Chem 277(13): 10795-10803.

Liu J, Chen H, Miller US, Saavedra JE, Keefer LK, Johnson DR, et al. 2001. Overexpression of glutathione S-transferase II and multidrug resistance transport proteins is associated with acquired tolerance to inorganic arsenic. Mol Pharmacol 60(2):302-309.

Liu Z, Carbrey JM, Agre P, Rosen BP. 2004. Arsenic trioxide uptake by human and rat aquaglyceroporins. Biochem Biophys Res Cemmun 316(4):1176-1185.

Liu Z, Shen J, Carbrey JM, Mukhopadhyay R, Agre P, Rosen BP. 2002. Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9. Proc Natl Acad Sci USA 99(9):6053-6058.

Meng YL, Liu Z, Rosen BP. 2004. As(III) and Sb(III) uptake by GlpF and efflux by ArsB in Esoherichia coli J Biol Chem 279: 18334-18341.

Nollert P, Harries WE, Fu D, Miercke LJ, Stroud RM 2001. Atomic structure of a glycerol channel and implications for substrate permeation in aqua(glycero)porins. FEBS Lett 504(3): 112-117.

Petrick JS, Jagadish B, Mash EA, Aposhian HV. 2001. Monomethylarsonous acid (MMA(III)) and arsenite: LD(50)in hamsters and in vitro inhibition of pyruvate dehydrogenase. Chem Res Toxicol 14(6):651-656.

Ramirez-Solis A, Mukopadhyay R, Rosen BP, Stemmler TL. 2004. Experimental and theoretical characterization of arsenite in water: insights into the coordination environment of As-O. Inorg Chem 43(9):2954-2959.

Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY:Cold Spring Harbor Laboratory Press.

Sanders 0I, Rensing C, Kuroda M, Mitra B, Rosen BP. 1997. Antimonite is accumulated by the glycerol facilitator GlpF in Escherichia coll. J Bacteriol 179(10):3365-3387.

Soignet SL, Maslak P, Wang ZG, Jhenwar S, Calleja E, Dardashti LJ, et al. 1996. Complete remission after treatment of acute promyelocytic leukemia with arsenic trioxide. N Engl J Med 339(19):1341-1348.

Sui H, Han BG, Lee JK, Walian P, Jap BK. 2001. Structural basis of water-specific transport through the AQP1 water channel. Nature 414(6866):872-678.

Tajkhorshid E, Nollert P, Jensen M0, Miercke LJ, O'Connell J, Stroud RM, et al. 2002. Control of the selectivity of the aquaporin water channel family by global orientational tuning. Science 296(5567):526-530.

Thomas DJ, Styblo M, Lin S. 2001. The cellular metabolism and systemic toxicity of arsenic. Toxicol Appl Pharmacol 176(2):127-144.

Vahter M. 2002. Mechanisms of arsenic biotransformation. Toxicology 181-182:211-217.

Walton FS, Harmon AW, Paul DS, Drobna Z, Patel YM, Styblo M. 2004. Inhibition of insulin-dependent glucose uptake by trivalent arsenicals: possible mechanism of arsenic-induced diabetes. Toxicol Appl Pharmacol 198(3):424-433.

Walton FS, Waters SB, Jolley SL, LeCluyse EL, Thomas DJ, Styblo M. 2003. Selenium compounds modulate the activity of recombinant rat AsIII-methyltransferase and the methylation of arsenlte by rat and human hepatocytes. Chem Res Toxicol 16(3):261-265.

Waters SB, Devesa V, Del Raze LM, Styble M, Thomas DJ. 2004a. Endogenous reductants support the catalytic function of recombinant rat cyt19, an arsenic methyltransferase. Chem Res Toxicol 17(3):404-409.

Waters SB, Devesa V, Fricke MW, Creed JT, Styblo M, Thomas DJ. 2004b. Glutathione modulates recombinant rat arsenic (+3 oxidation state) methyltransferase-catatyzed formation of trimethylarsine oxide and trimethylarsine. Chem Res Toxicol 17(12):1621-1629.

Wysocki R, Chery CC, Wawrzycka D, Van Hulle M, Cornelis R, Thevelein JM, et al. 2001. The glycerol channel Fps1p mediates the uptake of arsenite and antimonite in Saccharomyces cerevisiae. Mol Microbiol 40(6):1391-1401.

Yang HC, Cheng J, Finan TM, Rosen BP, Bhattacharjee H. 2005. Novel pathway for arsenic detoxification in the legume symbiont Sinorhizobium meliloti. J Bacteriol 187(20):6991-6997.

Zijuan Liu, (1) Miroslav Styblo, (2) and Barry P. Rosen (1)

(1) Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, Detroit, Michigan, USA; (2) Department of Nutrition, School of Public Health, and the Center for Environmental Medicine, Asthma, and Lung Biology, School of Medicine, University of North Carolina-Chapel Hill, Chapel Hill, North Carolina, USA

Address correspondence to B.P. Rosen, Department of Biochemistry and Molecular Biology, Wayne State University, School of Medicine, 540 E. Canfield Ave., Detroit, MI 48201 USA. Telephone: (313) 577-1512. Fax: (313) 577-2765. E-mail: brosen@med.wayne.edu

We thank W.R. Cullen for the generous gift of MAs(III).

This work was supported by National Institute of Health (NIH) grants ES10344 and GM55425 and a pilot project grant from the Wayne State University Environmental Health Sciences Center in Molecular and Cellular Toxicology with Human Applications (B.P.R.); American Heart Association postdoctoral fellowship 0520014Z (Z.L.); and NIH grant ES09941, U.S. Environmental Protection Agency cooperative agreement CR829522, and Clinical Nutrition Research Center grant DK 56350 (M.S.).

The authors declare they have no competing financial interests.
Table 1. Strains and plasmids.

Strains/plasmids Genotype/description

E. coli strains
 AW3110 [DELTA]arsRV(c):cam [F.sup.-]IN(rrnD-rrnE)
 OSBR1 AW3110 glpF:TnphoA, [Km.sup.r]
S. cerevisiae strains
 MG102 MAT[alpha] ura3-52 his6 1eu2-3,112
 his3-[DELTA]200 trp1-901 lys2-801
 suc2[DELTA]ycf1::hisG acr3::URA3
 HD9 MAT[alpha] ura3-52 his6 1eu2-3,112
 his3-[DELTA],200 trp1-901 lys2-801
 suc2[DELTA]ycf1::hisG acr3::URA3 fps1::leu2
Plasmids
 pGEM-T E. coli cloning vector, [Ap.sup.r]
 pYES3 S. cerevisiae--E. coli shuttle vector,
 [Ap.sup.r], TRP3
 pFPS1 1941-bp PCR fragment containing FPS1 cloned in
 pYES3
 pAQP9 1.1-kbp Hindlll-Kpnl fragment containing AQP9
 cloned into Hindlll-Kpnl-digested pYES3

Strains/plasmids Source

E. coli strains
 AW3110 Carlin et al. 1995
 OSBR1 Sanders et al. 1997
S. cerevisiae strains
 MG102 Ghosh et al. 1999
 HD9 Liu et al. 2002
Plasmids
 pGEM-T Promega (a)
 pYES3 Invitrogen (b)
 pFPS1 Present results
 pAQP9 Liu et al. 2002

(a) Promega Corp., Madison, WI. (b) Invitrogen Corp., Carlsbad, CA.
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Title Annotation:Research
Author:Rosen, Barry P.
Publication:Environmental Health Perspectives
Date:Apr 1, 2006
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