Printer Friendly

Metabolitos secundarios de Chaptalia meridensis.

Secondary metabolites from Chaptalia meridensis.

INTRODUCTION

Chaptalia, one of the seven recognized genera of the Gerbera-complex included in tribe Mutisieae, family Asteraceae [1], comprises ca. 65 species whose distribution range extends from south of United States to central Argentina, through Central America, Caribbean Islands, Andes Mountains and Brazil [2, 3]. Various species of this genus have been recognized in their countries of origin as medicinal plants. Particularly Ch. nutans is used in Argentinean traditional medicine as decongestant, antidepressant, anti-inflammatory, laxant and vulnerary [4,5]; in Brazilian folk medicine, this species is widely appreciated for its antifungal, anti-ulcer, anti-thrombotic and anti-inflammatory properties [6,7]. Many of these biological properties have been validated, since several crude extracts of this plant have shown to possess antifungal, antimicrobial and anti-inflammatory activities [6, 8, 9] , and also ability to inhibit edema induced by Bothrops asper snake venom [10].

According to literature consulted, few phytochemical works have been carried out with species of Chaptalia genus. To date, chemical composition of only two species, Ch. integerrima and Ch. nutans have been reported; the most common compounds isolated from these species are glucosyl-5methyl-furanocoumarins [9, 11, 12]; only once it has been detected in Ch. nutans the cyanogenic glucoside prunasin [13] as well as some valerolactones such as parasorbic acid and 5-methyl-3a-hydroxyvalerolactone [14].

As a continuation of our research on the phytochemistry of Venezuelan Andean Flora, we tackle the study of Chaptalia meridensis (Fig. 1), a small rosulate herb found commonly in Venezuelan Andean moor [15].

[FIGURE 1 OMITTED]

MATERIAL AND METHODS

General Experimental Procedures. Melting points were determined with a Fisher-Johns apparatus and they have not been corrected. Optical activities were measured on 60 Hz-Steeg & Reuter G.m.b.H. polarimeter using methanol or [CHCl.sub.3] as solvent. IR spectra were recorded on a Perkin-Elmer FT-1725X spectrophotometer as KBr pellets. [sup.1]H-, [sup.13]C- and two-dimensional NMR spectra were run with a Bruker-Avance DRX-400 instrument, using [CDCl.sub.3] as solvent. HRMS were acquired on a VG Micromass ZAB-2F. TLC was carried out on 0.25 mm layers of silica gel PF 254 (Merck); spots were visualized using UV light (254 and 365 nm) and subsequently by spraying with a mixture v/v [CH.sub.3]COOH-[H.sub.2]O-[H.sub.2][SO.sub.4] (20:4:1) and then heating with air flow at 100 [grados]C for few minutes.VCC was performed with silica gel 60 (70-230 mesh.).

Plant material. Plant material (leaves) was collected in "Paramo Las Nieves between the villages of Estaques and El Molino, Municipio Autonomo Arzobispo Chacon, Estado Merida, Venezuela" in December 2010. The botanical sample was identified as Chaptalia meridensis S. F. Blake by Eng. Juan Carmona Arzola, Department of Pharmacognosy and Organic Medicaments, Faculty of Pharmacy and Bioanalysis, University of Los Andes (ULA). A voucher specimen (J. M. Amaro-Luis, No 1648) was deposited at Herbarium MERF of this faculty.

Extraction and separation. Dry not crushed leaves ([congruente con] 2.4 Kg) were extracted at room temperature with dichloromethane and then with methanol in a soxhlet to give, respectively, 38 and 52 g of crude extracts. The dichloromethane extract was preadsorbed on silica gel 60 and chromatographed on the same adsorbent, using the Coll & Bowden vacuum liquid chromatography technique [16]. Column was eluted with hexane, dichloromethane, ethyl acetate and methanol in mixtures of increasing polarity. Sixty-four (64) fractions of 0.5 L were collected, concentrated in vacuo, and combined according to TLC similarity to afford nineteen (19) major fractions (A-R).

S-(+)-Marmesin (1): Combined major fraction "N" (fractions 49-53; [congruente con] 2.37 g) was rechromatographed on a silica gel column using hexane-ethyl acetate (3:2) to furnish an impure crystalline solid ([congruente con] 0.125 g), that was purified by preparative TLC (silica gel, two development in hexane-ethyl acetate 4:1). Crystallization from methanol provided pure white needles ([congruente con] 78 mg) detected in TLC plates as a blue-green fluorescence spot; m.p. = 187-188 [grados]C; [[alfa]]D: + 25.3[grados] (c, 0.38 methanol). IR (KBr), vmax ([cm.sup.-1]): 3448 (O-H), 3044 (=C-H), 2980 (C-H), 1702 (C=O), 1626 (C=C), 1158 and 1128 (C-O), 928 (=C-H). [sup.1]H NMR (Fig. 3). [sup.13]C NMR (Fig. 3); [[delta].sub.H] and [[delta].sub.C] consistent with those previously reported [17, 18]. HR-EI-MS: m/z (%) 246.0906 (55.28) [[M.sup.+]], 228.0777 (3.53) [[M.sup.+]-[H,sub.2]O], 213.0555 (21.43) [[M.sup.+]-[H.sub.2]O-[CH.sub.3]], 188.0464 (79.11), 187.0379 (100) [[M.sup.+]-HO-C-([[CH.sub.3]).sub.2]], 175.0403 (23.41), 160.0517 (40.45), 159.0445 (14.05), 131.0500 (9.92), 102.0469 (8.01), 77.0391 (13.40), 59-0519 (79.62).

Lagerenyl acetate (2): Combined major fraction "H" (fractions 25-26; [congruente con] 1.91 g) was filtered through a Sephadex LH-20 column eluted with hexane-C[H.sub.2][Cl.sub.2]-MeOH (1:2:2); sub-fraccions "[H.sub.6]" gave a solid which crystallized from methanol as white flakes ([congruente con] 52.0 mg); m.p. = 122-124 [grados]C; [[alfa]] D: +59.2[grados] (c, 0.44 ethanol). IR (KBr) vmax ([cm.sup.-1]): 2944-2364 (C-H), 1736 (C=O), 1712 (C=O), 1464 (C-H), 1244 (C-O) and 1036 (cyclopropil C-H). [sup.1]H NMR (Fig. 5), [sup.13]C NMR (Fig. 5); 8H and [delta]C comparable with those previously reported [19, 20]. HR-EI-MS: m/z (%) 484.0906 (24.06) [[M.sup.+]], 469.3488 (36.44) [[M.sup.+]-C[H.sub.3]], 424.3499 (45.26) [[M.sup.+]-C[H.sub.3]COOH], 409.3275 (100) [M+-C[H.sub.3]-COOH/-[CH.sub.3]], 395.3136 (14.17), 302.2504 (21.02), 297-2484 (17.10), 287.2276 (12.65), 203.1744 (25.82), 187.1437 (20.34), 175.1435 (36.95), 161.1278 (22.00), 147.1120 (23.43), 135.1115 (39.0), 127.1068 (32.80), 107.0807 (32.92), 95.0808 (40.55), 71.0476 (40.46).

[FIGURE 2 OMITTED]

Friedelin (3): Isolated from combined major fraction "F" (fractions 18-22; [congruente con] 2.65 g) by repeated silica gel column chromatography, eluted with different hexane-dichlorometane mixtures.; final purification, was carried out by crystallization from ethyl acetate/hexane, providing white needles (= 160.0 mg); m.p. = 267-267 [grados]C; [[alfa]] D: -22.5[grados] (c, 0.41 CH[Cl.sub.3]). Physical constants and data of IR, [sup.1]H NMR, [sup.13]C NMR and MS were comparable with those reported in the literature [21-23].

Friedelan-3[beta]-ol (4): Combined major fraction "E" (fractions 16-17; [congruente con] 3.39 g) was rechromatographed on a dry silica gel column eluted with hexanedichoromethane 3:2; from subfraction "[E.sub.4]" a white solid was obtained which was purified by crystallization in methanol, m.p. = 285-287 [grados]C; [[alfa]] d: +22.5[grados] (c, 0.38 CH[Cl.sub.3]). Its spectral data are in accordance with those described previously [23, 24].

Betulinic acid (5): Ethyl acetate solutions of combined major fractions "L", "M" and "N" (fractions 39-48) were pooled and evaporated to dryness to give a green residue ([congruente con] 4.72 g), which was filtered through a Sephadex LH-20 column packed in methanol; the eluted solution obtained was reduced "in vacuo" until a solid precipitated and this was then filtered off and recrystallized from methanol yielding pure white flakes; m.p. = 289-290 [grados]C; [[[alfa].sub.D]: + 8.0 (c, 0.29 methanol). These physical constants and the obtained spectral data are consistent with those previously published for betulinic acid [23, 25].

RESULTS AND DISCUSSION

Dichloromethane extract from leaves of Ch. meridensis was purified by standard procedures as detailed in previous section "materials and methods". This methodology led to the isolation and purification of five compounds, whose identity was established by 1D- and 2D-NMR studies and comparison of their spectral data with those reported in the literature.

Compound (1) was obtained as white needles (m.p. = 187-188 [grados]C; [[[alfa]].sub.D]: + 25.3[grados]). It showed in TLC on silicagel [GF.sub.254] plates a blue-green fluorescence spot, typical of a coumarin derivative [26], which is consistent with the presence in its UV spectrum of bands at [[lambda].sub.max]= 254 and 365 nm [27]. A molecular ion peak at m/z 246.0906 [[M.sup.+]] in its HR-EI-MS, in combination with [sup.13]C-NMR spectroscopic data, suggested the molecular formula [C.sub.14][H.sub.14][O.sub.4] with eight indices of hydrogen deficiency.

The IR spectrum showed absorptions for double bonds (3044, 1626 and 928[cm.sup.-1]), hydroxyl groups (3448 [cm.sup.-1]) and an [alfa], [beta]-unsaturated carbonyl group (1702 [cm.sup.-1]). This latter group was also characterized in the [sup.13]C-NMR spectrum, which displayed a carbonyl carbon signal at [[delta].sub.C] 161.6 [-O-C=O (C-2)], that together with the peaks of two substituted aromatic carbon at [[delta].sub.C] 155.8 [-O-C= (C-8a)] and [[delta].sub.C] 112.9 [>C= (C-4a)] and the signals of an olefin system [doublets at [[delta].sub.H] 6.17 and [[delta].sub.H] 7.57; J = 9.2 Hz (=CH / H-3 and H-4); HMQC: H-3 [fleche diestra y siniestra] C-3 ([[delta].sub.C] 112.3, =CH); H-4 [fleche diestra y siniestra] C-4 ([[delta].sub.H] 143.8; =CH)], conform the typical [alfa], [beta]-unsaturated [delta]-lactone of a coumarin [alfa]-pyron ring. The following HMBC correlations sequence C-3 [fleche diestra y siniestra] H-4 [fleche diestra y siniestra] C-4a [fleche diestra y siniestra] H-3 [fleche diestra y siniestra] C-2 [fleche diestra y siniestra] H-4 [fleche diestra y siniestra] C-8a (Fig. 3), confirm this structural subunit.

[FIGURE 3 OMITTED]

The [alfa]-pyron ring is condensed to a 1,2,4,5 tetrasubstituted benzene nucleus [singlets at [[delta].sub.H] 7.20 and [[delta].sub.H] 6.69 (=CH / H-5 and H-8); HMQC: H-5 [fleche diestra y siniestra] C-5 ([[delta].sub.C] 123.5, =CH); H-8 [fleche diestra y siniestra] C-8 ([[delta].sub.C] 98.0; =CH)] through the carbons C-8a and C-4a, according to HMBC cross-peaks C-8a [fleche diestra y siniestra] H-5 [fleche diestra y siniestra] C-4a [fleche diestra y siniestra] H-8 (Fig. 3). The presence in the molecule of a third structural subunit, integrated by a 2-substituted dihydrofuran ring [[[delta].sub.H] 3.21, m, (>[CH.sub.2]; H-3') and [[delta].sub.H] 4.72, t, J = 8.6 Hz (>CH-O-; H-2'); HMQC: H-3' [fleche diestra y siniestra] C-3' ([[delta.sub.C] 29.6, >CH2); H-2' [fleche diestra y siniestra] C-2' ([[delta].sub.C] 91.3; (>CH-O-)], which is linked to benzene nucleus through the carbons C-6 ([[delta].sub.C] 125.2; >C=) and C-7 ([[delta].sub.C] 163.3; -O-C=), assemble a molecular structure of a linear dihydropyranocoumarin. The substituent at C-2' in dihydrofuran ring was identified as 1-hydroxy-1 methylethyl moiety, which is consistent with the existence of a base peak at m/z: 187.0379 [[C.sub.11][H.sub.7][O.sub.3]/[M.sup.+]HO-C-([[CH.sub.3]).sub.2]] in the HR-EI-MS, as well as with NMR data [two methyl singlets at [[delta].sub.H] 1.22 and [[delta].sub.H] 1.36 ([H.sub.3]C-C(OH)-[CH.sub.3]), a hydroxyl proton at [[delta].sub.H] 1.91 (s, -OH), two methyl carbon peaks at [[delta].sub.C] 24.5 (C-5') and [[delta].sub.C] 26.2 (C-6'), a quaternary oxy-carbon peak at [[delta].sub.C] 71.8 (-O-C<; C-4') and the following HMBC correlations:C-2' [fleche diestra y siniestra] H-5' [fleche diestra y siniestra] C-4' [fleche diestra y siniestra] H-6' [fleche diestra y siniestra] C-2' [fleche diestra y siniestra] H-3' [fleche diestra y siniestra] C-4' ; C-5' [fleche diestra y siniestra] H-6' and H-2' [fleche diestra y siniestra] C-6' [fleche diestra y siniestra] H- 5'] (Fig 3). Consequently, the above analysis led to the gross structure (1) (C-2' [xi]) named 2,3-dihydro2-(1-hydro xy-1-methylethyl)-7H-furo[3,2 g][1] benzopyran-7-one; this structure is assigned in the scientific literature to (+)-marmesin or to (-)-nodakenetin, depending on whether the configuration at C-2' is S or R, respectively [28]. The isolated compound is dextrorotatory and it is therefore S-(+)-marmesin (1), in which the C-2' substituent has an equatorial orientation [29, 30]. This coumarin was isolated for the first time from the bark of Aegle marmelo [31] and has subsequently been found in many species, particularly those included in Rutaceae and Apiaceae families [32, 33]. The biological and pharmacological interest of marmesin, as well as that of many other furocoumarins is well illustrated in the literature [18, 34-38].

Compound (2): White flakes, m.p. = 122-124 [grados]C; [[alfa]] D: +59.2[grados]. The presence in its HR-EIMS of an ion molecular peak at m/z: 484.3734 in conjunction with NMR data, allowed to establish the molecular formula [C.sub.32][H.sub.52][O.sub.3], which includes seven unsaturation degrees. Its IR spectrum showed typical bands of an aliphatic ester [[v.sub.max]: 1736 (C=O) and [v.sub.max]: 1244 and 1464 [cm.sup.-1] (C-O-C)], a ketone (1712 [cm.sup.-1]), and a cyclopropane ring (1036 [cm.sup.-1]). The [sup.1]H NMR spectrum shows four angular methyl singlets at [[delta].sub.H] 0.88 (H-28), 0.84 (H-29), 0.89 (H-30) and 0.95 (H-18), two overlapping methyl doublets at [[delta].sub.H] 1.09, J = 6.8 Hz (H-26 and H-27), a third methyl doublet [[delta].sub.H] 0.85, J = 6.4 Hz (H-21) and two mutually coupled proton doublets of a cyclopropane ring at [[delta].sub.H] 0.33 and 0.57, J = 4.2 Hz (>C[H.sub.2]; H-19), suggesting a cycloartane triterpene skeleton. The presence in the molecule of an acetoxy group located at C-3, was made evident in the HR-EI-MS [prominent fragments at m/z: 424.3499 [[M.sup.+]-[CH.sub.3]COOH] and m/z: 400.3275 [M+-C[H.sub.3]COOH/-C[H.sub.3], base peak] and also in 1D and 2D-NMR spectra [methyl singlet at [[delta].sub.H] 2.05 (H-32) HMQC: [fleche diestra y siniestra] [[delta].sub.C] 21.5 (C-32); peak at [[delta].sub.C] 171.1 (-O-C=O; C-31); double doublet at [[delta].sub.H] 4.56, J = 11.2 and 5.6 Hz (>CH-O-; H-3) HMQC: [fleche diestra y siniestra] [[delta].sub.C] 80.8 (>CH-O-; C-3) and HMBC correlations: H-3 [fleche diestra y siniestra] C-31 [fleche diestra y siniestra] H-32 (Fig. 5)]; the b-equatorial orientation of this acetoxy group was supported on the multiplicity of H-3 carbinyl hydrogen, which clearly implies axial-axial and axial-equatorial types coupling with C-2 methylene protons. Ketone carbonyl group [[[delta].sub.C] 215.5 (>C=O; C-24) was located in the side chain of cycloartane skeleton, on the carbon adjacent to the terminal isopropy group, as it was inferred from NMR data [[sup.1]H, [sup.1]H-COSY correlations between a septet at [[delta].sub.H] 2.61, J = 6.8 Hz; (>CH (H-25) and two overlapping methyl doublets at [[delta].sub.H] 1.09, J = 6.8 Hz (H-26 and H-27); 13C NMR: [[delta].sub.C] 40.9 (>CH ; C-25) and [[delta].sub.C] 16.5 (2-C[H.sub.3]; C-26 and C-27) ; HMBC cross coupling: C-24 [fleche diestra y siniestra] H-25, H-26 and H-27; C-25 [fleche diestra y siniestra] H-26 and H-27; C-26 [fleche diestra y siniestra] H-25 and H-27; C-27 [fleche diestra y siniestra] H-25 and H-26] (Fig 5). The [beta]-axial orientation of the side chain was determined through NOESY spectrum, where NOE effects involving H-17([alfa])/[H.sub.3]0([alfa]); H-17([alfa])/H-21 and H-18([beta])/H-20 were observed; similarly NOESY correlation between H-3([alfa])/H-5([alfa]), Ha-19/H-29([beta]), Hb-19/H-8([beta]) and H-8/H-18([beta]) confirm a trans A/B, cis B/C and trans C/D ring junctions, typical in natural cycloartane triterpenes (Fig. 4).

[FIGURE 4 OMITTED]

In conclusion, the preceding data confirm that structure (2) corresponds to 3[beta]-acetoxy-17[alfa]H-cycloartan-24-one, which it is commonly known as lagerenyl acetate. Up to now, this compound has been isolated only of two species, both included in genus Lagerstroemia: L. lancasteri [19] and L. speciosa [20] (Lytharaceae). Its 2D NMR spectral study is reported here for first time. Recently a biological study focused on human CYP3A4 promoter activity of this compound and its deacetyl derivative has been published [39].

[FIGURE 5 OMITTED]

Detailed analysis of spectral data of compounds (3), (4) and (5) (see materials and methods section) indicates that they also are triterpenoids. Comparison of the NMR data with those described in the literature, confirmed the identity of these compounds as friedelin (3) [21-23], friedelan-3p-ol (4) [23,24] and betulinic acid (5) [23,25]. These triterpenoids are widely distributed in plant kingdom [40-43], but up to now they have not been reported in the genus Chaptalia. Their potential biological activity is well documented in the literature [23, 24, 44-53].

CONCLUSIONS

In this first phytochemical study of the Venezuelan endemic species Chaptalia meridensis, have been isolated and identified four triterpenoids and a linear dihydrofuran-coumarin It is the first time that this type of compounds is reported in the genus Chaptalia. A remarkable aspect of all isolates compounds is their wide range of biological activities.

From a chemotaxonomic point of view, it has highlighted the presence of 5-methylcoumarins in the subtribe Mutisiinae [54, 55], in which it is included Chaptalia [9, 10]. However, this study and other previously reported by Zottis et al. [11] show that Chaptalia species produce a greater variety of coumarins.

It is highly significant presence in this specie of uncommon cycloartane triterpene lagerenyl acetate (2), whose spectral study using two-dimensional NMR techniques is performed here by first time.

ACKNOWLEDGMENTS

The authors are grateful to Venezuelan Ministry of Popular Power for Science, Technology and Innovation (MCTI), "Science Mission" Program for financial support Grant No 2008000937). We would like to thank Prof Angel G. Ravelo, Instituto Universitario de Bio-Organica, Universidad de La Laguna. Tenerife, Spain, for the HR-EI-MS. Thank are also due to Eng. Juan Carmona Arzola, Department of Pharmacognosy and Organic Medicaments, Faculty of Pharmacy and Bioanalysis, University of Los Andes (ULA) for identification of plant material.

BIBLIOGRAPHIC RE FERENCE S

[1] Katinas L. The Gerbera complex (Asteraceae: Mutisieae): To split or not to split. Sida. 2004; 21(2): 935-940.

[2] Nesom GL. Revision of Chaptalia (Asteraceae: Mutisieae) from North America and continental Central America. Phytologia. 1995; 78(3): 153-188.

[3] Katinas L, Zavaro C. Endemism and taxonomy of Chaptalia (Asteraceae) in the Caribbean. I. Introduction and morphology. Ann Bot Fenn. 2014; 51(4): 240-252.

[4] Barboza GE, Cantero JJ, Nunez C, Pacciaroni A, Ariza Espinar L. Medicinal plants: A general review and a phytochemical and ethnopharmacological screening of the native Argentine Flora. Kurtziana. 2009; 34(1-2): 7-387.

[5] Keller H. "No soy feliz": Origen, usos y agencia social de Chaptalia nutans (Asteraceae), segun los guaranies de Misiones, Argentina. Bonplandia. 2013; 22(2): 171-180.

[6] Coelho de Souza G, Haas APS, von Poser GL, Schapoval EES, Elisabetsky A. Ethnopharmacological studies of antimicrobial remedies in the south of Brazil. J Ethnopharmacol. 2004; 90(1): 135-143.

[7] Fenner R, Betti AH, Mentz LA, Kuze Rates SM. Plantas utilizadas na medicina popular brasileira com potencial atividade antifungica. Braz J Pharm Sci. 2006; 42(3): 369-394.

[8] Heinrich M, Kuhnt M, Wright CW, Rimpler H, Phillipson JD, Schandelmaier A, Warhurst DC. Parasitological and microbiological evaluation of Mixe Indian medicinal plants (Mexico). J Ethnopharmacol. 1992; 36(1): 81-85.

[9] Torrado Truiti MC, Sarragiotto MH, Alves de Abreu Filho B, Nakamura CV, Dias Filho BP. In vitro antibacterial activity of a 7-O-P-dglucopiranosyl-nutacoumarin from Chaptalia nutans (Asteraceae). Mem Inst Oswaldo Cruz. 2003; 98(2): 283-286.

[10] Torrado Truiti MC, Sarragiotto MH. Three 5-methylcoumarins from Chaptalia nutans. Phytochemistry. 1998; 47(1): 97-99.

[11] Zottis A, Vidotti GJ, Sarragiotto MH. Coumarins from Chaptalia integerrima. Biochem Syst Ecol. 2001; 29(7): 755-757.

[12] Fikenscher LH, Hegnauer R. Cyanogenesis in Cormophytes. 12. Chaptalia nutans, a strongly cyanogenic plant of Brazil. Plant Med. 1977; 31(2): 266-269.

[14] Dominguez XA, Jakupovic J, Sanchez VH., del Rio SE. Valerolactones from Chaptalia nutans. Rev Latinoam Quim. 1988; 19(2): 92-93.

[15] Briceno B, Morillo G. Catalogo abreviado de las plantas con flores de los paramos de Venezuela. Parte I. Dicotiledoneas (Magnoliopsida). Acta Bot Venez. 2002; 25(1): 1-46.

[16] Coll JC, Bowden BF. The application of vacuum liquid chromatography to the separation of terpene mixtures. J Nat Prod. 1986; 49(5): 934-936.

[17] Nemoto T, Ohshima T, Shibasaki M. Enantioselective total syntheses of (+)-decursin and related natural compounds using catalytic asymmetric epoxidation of an enone. Tetrahedron. 2003; 59(35): 6889-6897.

[18] Ma Y, Jung JY, Jung YJ, Choi JH, Jeong WS, Song YS, Kang JS, Bi K, and Kim MJ. Antiinflammatory activities of coumarins isolated from Angelica gigas Nakai on LPS-stimulated RAW 264.7 cells. J Food Sci Nutr. 2009; 14(3): 179-187.

[19] Talapatra B, Chaudhuri PK, Mallik AK, Talapatra SK. Lagerenyl acetate and lagerenol, two tetracyclic triterpenoids with the cycloartane skeleton from Lagerstroemia lancasteri. Phytochemistry; 1983; 22(11): 2559-2562.

[20] Ragasa CY, Ngo HT, Rideout JA.Terpenoids and sterols from Lagerstroemia speciosa. J Asian Nat Prod Res. 2005; 7(1): 7-12.

[21] Triterpenoid ketones from Lingnania chungi McClure: Arborinine, friedelin and glutinone Akihisa T, Yamamoto K, Tamura T, Kimura Y, Iida T, Nambara T, Chang FC. Chem Pharm Bull. 1992; 40(3):789-791.

[22] De Oliveira DM, da Nova Mussel W, Duarte LP, Silva GDF, Duarte HA, de Lima Gomes HC, Guimaraes L, Vieira Filho SA. Combined experimental powder X-ray diffraction and DFT data to obtain the lowest energy molecular conformation of friedelin. Quim Nova. 2012; 35(10): 1916-1921.

[23] Monkodkaew S, Loetchutinat C, Nuntasaen N, Pompimon W. Identification and antiproliferative activity evaluation of a series of triterpenoids isolated from Flueggea virosa (Roxb. ex Willd.). Am J Appl Sci. 2009; 6(10): 1800-1806.

[24] Kundu JK, Rouf ASS, Hossain MN, Hasan CM, Rashidu MA. Antitumor activity of epifriedelanol from Vitis trifolia. Fitoterapia. 2000; 71(5): 577-579.

[25] Peng C, Bodenhausen G, Qiu S, Fong HHS, Farnsworth NR, Yuan S, Zheng C. Computerassisted structure elucidation: Application of CISOC-SES to the resonance assignment and structure generation of betulinic acid. Magn Res Chem. 1998; 36(4): 267-278.

[26] Wagner H, Bladt S. Plant drug analysis-A thin layer chromatography atlas. Chapt 5-Coumarin drugs. 2nd ed. New York: Springer-Verlag; 1996. p 125.

[27] Gonzalez AG, Barroso JT, Z. D. Jorge ZD, Rodriguez Luis F. Espectroscopia ultravioleta de cumarinas. Interpretacion y correlaciones experimentales. Rev Real Acad Cienc Exact Fis Nat Madrid. 1981; 75(4): 811-855.

[28] Nielsen BE, Lemmich J. Constituents of Umbelliferous plants V. On the configuration of archangelicin and related coumarins. Acta Chem Scand. 1964; 18(9): 2111-2114.

[29] Harada I, Hirose Y, Nakazaki M. The absolute configurations of (+)-marmesin and (-)- hydroxytrementone. Tetrah Lett. 1968; 9(52): 5463-5466.

[30] Goswami S, Gupta VK, SharmaA, Gupta BD. Supramolecular structure of S-(+)-marmesin-a linear dihydrofuranocoumarin. Bull Mater Sci. 2005; 28(7): 725-729.

[31] Chatterjee A, Mitra SS. On the constitution of the active principles isolated from the matured bark of Aegle marmelos, Correa. J Am Chem Soc. 1949; 71(2): 606-609.

[32] Murray RDH, Mendez J, Brown SA. Natural coumarins: Occurrence, chemistry and biochemistry. New York: Wiley; 1982.

[33] Murray RDH. Naturally occurring plant coumarins, In, Herz V, Falk H, Kirby GW, Moore RE. (eds). Progress in the chemistry of organic natural products, Vol 83. Wien: Springer-Verlag; 2002. p 1-663.

[34] Uwaifo AO, Heidelberg C. Photobiological activity of marmesin (5-B-hydroxyisopropyl-4-5 dihydrofurocoumarin) in chinese hamster V79 cells. Photochem Photobiol. 1983; 38(4): 395-398.

[35] Trumble JT, Millar JG. Biological activity of marmesin and demethylsuberosin against a generalist herbivore, Spodoptera exigua (Lepidoptera: Noctuidae). J Agric Food Chem. 1996; 44(9): 2859-2864.

[36] Afek U, Orenstein J, Carmeli S, Aharoni N. Marmesin, a new phytoalexin associated with resistance of parsley to pathogens after harvesting. Postharv Biol Technol. 2002; 24(1): 89-92.

[37] Vimal V, Devaki T Linear furanocoumarin protects rat myocardium against lipidperoxidation and membrane damage during experimental myocardial injury. Biomed Pharmacother. 2004; 58(6): 393-400.

[38] Jain M, Kapadia R, Jadeja RN, Thounaojam MC, Devkar RV, Mishra SH. Hepatoprotective activity of Feronia limonia root. J Pharm Pharmacol. 2012; 64(6): 888-896.

[39] Kuang X, Li W, Kanno Y, Mochizuki M, Inouye Y, Koike K.Cycloartane-type triterpenes from Euphorbia fischeriana stimulate human CYP3A4 promoter activity. Bioorg Med Chem Lett. 2014; 24(23): 5423-5427.

[40] Chandler RF, Hooper SN. Friedelin and associated triterpenoids. Phytochemistry. 1979; 18(5): 771-724.

[41] Pai SR, Joshi RK. Distribution of betulinic acid in plant kingdom. PST. 2014; 1(3): 103-107.

[42] Glasby JS. Dictionary of plants containing secondary metabolites. London: Taylor & Francis; 2005.

[43] Connolly JD, Hill RA. Dictionary of terpenoids, Vol 2: Di- and higher terpenoids; p 1341 Friedelanes. London: Chapman & Hall; 1991.

[44] Yogeeswari P, Sriram D. Betulinic acid and its derivatives: A review on their biological properties. Curr Med Chem. 2005; 12(6): 657-666.

[45] Torres-Romero D, King-Diaz B, Strasser RJ, Jimenez IA, Lotina-Hennsen B, Bazzocchi IL. Friedelane Triterpenes from Celastrus vulcanicola as Photosynthetic Inhibitors. J Agric Food Chem. 2010; 58(20): 19847-10854.

[46] Ogunnusi TA, Oso BA, Dosumu OO.Isolation and antibacterial activity of triterpenes from Euphorbia kamerunica Pax. Int J Biol Chem Sci. 2010; 4(1): 158-167.

[47] Yang HH, Son2 JK, Jung B, Zheng MS, Kim JR. Epifriedelanol from the root bark of Ulmus davidiana inhibits cellular senescence in human primary cells. Planta Med. 2011; 77(5): 441-449.

[48] Csupor-Loffler B, Hajdu Z, I Zupko I, Molnar J, Forgo P, Vasas S, Kele Z, Hohmann J. Antiproliferative constituents of the roots of Conyza canadensis. Planta Med, 2011; 77(11): 1183-1188.

[49] Nugroho AE, Susidarti RA, Astuti P. Effects of friedelin from Eugenia chlorantha Duthie on physiological receptors-operated guinea-pig trachea contraction. J Pharm Res Clin Pract. 2011; 1(2):71-78.

[50] Mann A, Ibrahim K, Oyewale AO, Amupitan JO, Fatope MO, Okogun JI. Antimycobacterial friedelane-terpenoid from the root bark of Terminalia avicennioides. Am J Chem. 2011; 1(2): 52-55.

[51] Moghaddam MG, Ahmad FBH, SamzadehKermani A. Biological activity of betulinic acid: A review. Pharmacol Pharm. 2012; 3(2): 119-123.

[52] Prabhu A, Krrishnamoorthy M, Prasad J, Nail P. Anticancer activity of friedelin Isolated from ethanolic leaf extract of Cassia tora on HeLa and HSC-1 CELL Lines. Indian J Appl Res. 2013; 3(10):1-4.

[53] Lee SY, Kim HH, Park SU. Recent studies on betulinic acid and its biological and pharmacological activity. EXCLI J. 2015; 14(2):199-203.

[54] Zdero C, F. Bohlmann F, King RM, Robinson H. Further 5-methyl coumarins and other constituents from the subtribe Mutisiinae. Phytochemistry. 1986; 25(2): 509-516.

[55] Zdero C, F. Bohlmann F, Solomon J. Further 5-methylcoumarin derivatives from Mutisia orbignyana. Phytochemistry. 1988; 27(3): 891-897.

Pinto Ana Andreina, Amaro-Luis Juan Manuel *.

Laboratorio de Productos Naturales, Departamento de Quimica, Facultad de Ciencias, Universidad de Los Andes (ULA), Merida C.P. 5101, Republica Bolivariana de Venezuela .

Recibido mayo 2015--Aceptado junio 2015
COPYRIGHT 2015 Universidad de Los Andes, Facultad de Farmacia
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2015 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:texto en ingles
Author:Pinto, Ana Andreina; Amaro-Luis, Juan Manuel
Publication:Revista de la Facultad de Farmacia
Date:Jan 1, 2015
Words:4524
Previous Article:Estudio de la composicion quimica del aceite esencial de la Asteraceae hibrida Carramboa tachirensis (Aristeg.) Cuatrec.
Next Article:Hacia una seguridad y soberania alimentaria, prioridad fundamental de los pueblos?
Topics:

Terms of use | Privacy policy | Copyright © 2019 Farlex, Inc. | Feedback | For webmasters