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Marked susceptibility of South African Helicobacter pylori strains to ciprofloxacin and amoxicillin: clinical implications.

Helicobacter pylori is the principal species of the genus Helicobacter that inhabits the gastric mucosa of the human stomach. It chronically infects billions of people worldwide (1,2) and causes one of the most frequent chronic bacterial infections, involving more than 50% of the world's population. (3) H. pylori is one of the most genetically diverse of bacterial species, and is a major cause of at least 90% of duodenal ulcers, 70% of gastric ulcers, non-ulcer dyspepsia, gastro-oesophageal reflux disease, adenocarcinoma of the distal stomach and mucosa-associated lymphoid tissue (MALT) lymphoma in many societies. (4)

H. pylori infection presents a unique therapeutic challenge. Determining the optimal treatment is difficult because the organism lives in an environment not easily accessible to many medications. Several antibiotic agents are used worldwide to treat these infections; despite this range of antibiotics, the problem of drug resistance to most of them is well established. (2,5)

Reliable treatment of H. pylori infection is difficult, and successful regimens generally require two or more antibiotics coupled with a proton pump inhibitor. (6) Eradication of the organism is the first therapeutic approach and constitutes a reliable long-term prophylaxis of peptic ulcer relapse, accelerating ulcer healing and reducing the rate of ulcer complications. (4) However, the prevalence of multidrug-resistant strains, especially in developing countries, (7) makes it obligatory to perform culture and antibiotic sensitivity for patients with persistent infection after failure of initial or repeated treatment. (1)

Antibiotic resistance is a major cause of treatment failure. Primary resistance against clarithromycin and metronidazole is common in many countries, (5,8) while resistance to different antibiotics used as first-line treatment varies between countries and communities, and may change with time and geographical location. (1) In South Africa, the regimen for treating H. pylori infection comprises amoxicillin, metronidazole, clarithromycin and doxycycline (personal communication) and theoretically should demonstrate 85 - 95% efficacy. (9)

We recently identified H. pylori isolates from patients presenting with gastric diseases in the Eastern Cape. The organism is associated with substantial morbidity and mortality. (2) An increase in treatment failure is emerging because of growing resistance to currently used antibiotics and the fact that the antibiotic susceptibility patterns of micro-organisms change with time and geographical location. (1) Consequently, it is imperative to evaluate current susceptibility/resistance patterns of H. pylori strains in the Eastern Cape province in particular and South Africa in general, especially as existing studies are few and outdated, (10,11) to guide empirical treatment and prevent the emergence of further resistance. We investigated the susceptibility and resistance patterns of these isolates to seven different antibiotics.


Bacterial strains

Two hundred H. pylori strains were isolated from patients suffering from gastric diseases and referred to Livingstone Hospital, Port Elizabeth, for endoscopy. Biopsies were taken after informed consent. The study was approved by the institutional review board of the University of Fort Hare and the Eastern Cape Department of Health (Protocol number EcDoH-Res 0002).

The organism was isolated and identified as previously reported. (1) Biopsies were homogenised under aseptic conditions in 0.2 g/l of cysteine and 20% glycerol in brain-heart infusion broth and a loop full-plated primarily on freshly prepared Columbia agar base (Oxoid, Basingstoke, England) supplemented with 7% sheep's blood (Oxoid, England) and Skirrow's supplement (Oxoid, England); trimethoprim (2.5 mg), vancomycin (5 mg), cefsulodin (2.5 mg), and amphotericin (2.5 mg) were also added to the medium. All plates were incubated at 37[degrees]C for 3 - 5 days under micro-aerophilic conditions (5 - 6% [O.sub.2], 10% C[O.sub.2], 80 - 85% [N.sub.2]) (Anaerocult, Basingstoke, England). Isolates were identified based on colony morphology and positive oxidase, urease and catalase tests. A reference strain of H. pylori (NCTC 11638) was included as a positive control. Confirmed isolates were suspended in 20% glycerol and stored at -80[degrees]C in a freezer (Sanyo, Japan) for future experiments.

Antibiotic susceptibility testing

Susceptibility testing to determine zones of inhibition and minimum inhibitory concentration (MIC) was by the disk diffusion (Kirby-Bauer) technique and agar dilution methods respectively, which conform to the standard of the Clinical and Laboratory Standard Institute (CLSI)12 with little modification. (1) Antibiotics usually indicated for triple therapy, namely metronidazole (5 [micro]g), clarithromycin (15 [micro]g), tetracycline (10 [micro]g), amoxicillin (10 [micro]g), gentamicin (10 [micro]g), ciprofloxacin (5 [micro]g) and erythromycin (10 [micro]g) (Mast Diagnostics, Bootle, England), were used. This selection was based on the current treatment regimen in South Africa (personal communication) and elsewhere. (6) Brain-heart infusion (BHI) agar (Oxoid, England) containing 7% horse blood and H. pylori-selective supplement (Oxoid, England) was used. H. pylori control strain NCTC 11638 was included in all the experiments to determine susceptibility or resistance. (1) The zones of inhibition were interpreted as previously reported. (1,13)

For the MIC values, susceptibility for clarithromycin was interpreted according to the CLSI guideline. (12) Since the CLSI has not designated breakpoints for H. pylori for all the antibiotics commonly used for treatment, the resistant breakpoint used for metronidazole, gentamicin and erythromycin was [greater than or equal to]8 [micro]g/ml; [greater than or equal to]2 [micro]g/ml was used for tetracycline, >1.0 [micro]g/ml for clarithromycin, and [greater than or equal to]1.0 [micro]g/ml for ciprofloxacin and amoxicillin. (12,13)

Statistical analysis

The Epi Info 2000 software package (Centers for Disease Control and Prevention, Atlanta, USA) was used for statistical analysis. Comparisons of antimicrobial resistance rates with the sex of the patients were determined using the chi-square test and a p-value of <0.05 was considered significant.


Antimicrobial patterns

Of the 200 strains subjected to the antibiotics, 100% susceptibility was recorded for ciprofloxacin and 97.5% for amoxicillin. Marked resistance was noted for metronidazole 95.5% (Table I).

Of the 200 strains tested, 67 (33.5%) were from males and 133 (66.5%) from females. The prevalence of metronidazole resistance in females and males was 125/191 (65.44%) and 66/191 (34.55%) respectively, while for erythromycin it was 61/89 (68.53%) and 28/89 (31.46%) respectively (Fig. 1).

There was a higher prevalence of resistant isolates in female compared with male patients, that did not reach statistical significance (p>0.05).

MIC determination

Of the 7 antibiotics, metronidazole showed no MIC within the susceptible breakpoint range. MIC values for the other antibiotics were 0.0625 - 1.0 [micro]g/ml for ciprofloxacin, 0.125 - 1.0 [micro]g/ml for clarithromycin, 1.25 - 2.0 [micro]g/ml for tetracycline, and 2.5 - 5 [micro]g/ml for amoxicillin (Table I).


The resistance of H. pylori to antibiotic treatment regimens is a growing problem, (14) and in several African countries is largely linked to misuse of antibiotics. (1) Our results revealed marked antimicrobial susceptibility rates of 100% for ciprofloxacin, and 97.5% for amoxicillin, in line with other investigators. (6,15) Hung et al. (6) documented 94.3% susceptibility of their strains to ciprofloxacin, and Kohanteb et al. (15) reported 95.3% for theirs. The latter authors also reported very high rates of amoxicillin susceptibility ranging from 79 - 99%. Although the rate of amoxicillin resistance is low in this study, and was absent for ciprofloxacin, resistance for both drugs has been demonstrated in different parts of the world. (16)


A similar study in western Nigeria documented 100% resistance by H. pylori strains to amoxicillin. (7) We theorise that this could be related to the differences in local antibiotic prescription practices and use. (2) H. pylori has been reported to lose the resistant phenotype to amoxicillin because of freezing and storage, (14) which most probably occurred in the different studies; this may also help to explain the observed differences. (1)

Amoxicillin resistance is thought to develop because of structural alterations in the penicillin-binding protein, or changes in proteins involved in cell wall synthesis. (17) The possibility of bacterial strains acquiring resistance to amoxicillin is therefore strong. Colonisation of the stomach with [beta]-lactam-resistant bacteria may lead to the transfer of amoxicillin-resistance to H. pylori.

Our isolates were 80% susceptible to clarithromycin, 72.5% to gentamicin, 67.5% to tetracycline and 55.5% to erythromycin. Similar results were found in Ethiopia, Cameroon and Kenya, reporting very good activity of these antibiotics against H. pylori isolates. (1) Others, however, have reported resistance to clarithromycin. (14) Although clarithromycin is an expensive macrolide, cross-resistance linked with the use of other less expensive macrolides may be responsible for this resistance, since it is less abused. (14) However, clarithromycin-susceptible and resistant strains have been isolated from patients with no history of exposure to macrolides, (18) which may suggest that administration of clarithromycin may select for the resistant strains and therefore must be guided by empirical treatment.

We observed very high resistance (95.5%) to metronidazole, similarly to a study in Nigeria (100% resistance). (7) Metronidazole resistance has been reported in 10-50% of all adult patients infected with H. pylori in developed countries, (19) and virtually all strains are resistant in the developing countries. (14) The high prevalence of metronidazole resistance in developing countries has been linked to the frequent use of nitroimidazole derivatives for the treatment of protozoal infections and gynaecological problems. (1,2,14) This resistance is thought to be mostly because of genetic mutations in the RdxA and FrxA genes. (5)

We found no significant difference (p<0.05) in the prevalence of antibiotic-resistant strains among females and males, although there was a remarkable difference in metronidazole resistance between the sexes, as seen in other studies. (14) However, the higher resistance observed with metronidazole in females may be related to its use in treating trichomoniasis and bacterial vaginosis, which is common in most developing countries. (1,14)

In our study, MIC values for the antibiotics that demonstrated susceptibility to the isolates were 0.125 - 1.0 [micro]g/ml for clarithromycin, 1.25 - 2.0 [micro]g/ml for tetracycline, 2.5 - 5.0 [micro]g/ml for amoxicillin,; 5 - 8.0 [micro]g/ml for gentamicin, 2.5 - 5.0 [micro]g/ml for erythromycin, and 0.625 - 1.0 [micro]g/ml for ciprofloxacin. These values are similar to those found in other studies, (14) and are in agreement with CLSI-approved quality control ranges for H. pylori. (12,13)


We commonly encountered multidrug resistance of clinical significance that calls for continuous surveillance of antibiograms to guide empirical treatment. We advocate the inclusion of ciprofloxacin in the treatment regimen of H. pylori infection in our study environment. Our results also indicate the need to establish baseline susceptibility data for empirical treatment of cases and the conducting of studies involving newer and broad-spectrum antibiotics to address resistance.

We are grateful to the National Research Foundation of South Africa (grant reference 69815) and Govan Mbeki Research and Development Centre, University of Fort Hare, Alice, for financial support, and the staff of the GIT Unit, Livingstone Hospital, Port Elizabeth, and Mr B Clarke, for technical assistance.

Accepted 19 June 2009.


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(2.) Tanih NF, Dube C, Green E, et al. An African perspective on Helicobacter pylori prevalence, drug resistance and alternative approaches to treatment. Ann Trop Med Parasitol 2009; 103(3): 189-204.

(3.) Pounder RE, Ng D. The prevalence of Helicobacter pylori infection in different countries. Aliment Pharmacol Ther 1995; 9 (suppl 2): 33-39.

(4.) NIH consensus Development Panel on Helicobacter pylori in peptic ulcer disease. Helicobacter in peptic ulcer disease. JAMA 1994; 272: 65-69.

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(16.) Quintana-Guzman, EM, Arias-Echandi, ML, Salas-Chaves P, Davidovich-Rose H, Schosinsky-Neverman K. Helicobacter pylori: susceptibility to amoxycillin, erythromycin, tetracycline, ciprofloxacine, nitrofurantoin and metronidazole in Costa Rica. Rev Biomed 1998; 9: 92-96.

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Department of Biochemistry and Microbiology, University of Fort Hare, Alice, E Cape

Nicoline F Tanih, MSc

Benjamin I Okeleye, BSc (Hons)

Anna M Clarke, PhD

Noxolo Mkwetshana, PhD

Ezekiel Green, MSc

Roland N Ndip, PhD

Department of Gastroenterology, Livingstone Hospital, Port Elizabeth, E Cape Nathan Naidoo, MB ChB, FCP (SA)

Department of Biochemistry and Microbiology, Faculty of Science, University of

Buea, Buea, Cameroon

Lucy M Ndip, PhD

Roland N Ndip, PhD

Corresponding author: R Ndip (
Table I. Antibiotic sensitivity results of H. pylori strains
isolated from gastric biopsy specimens

 Antrum Corpus Antrum
Antibiotics No sus (%) No sus (%) No res (%)

Clarithromycin 87 (82.07) 73 (77.65) 19 (17.92)
Tetracycline 69 (66.34) 66 (68.75) 35 (33.65)
Amoxicillin 103 (98.09) 92 (96.84) 2 (1.90)
Metronidazole 6 (5.94) 3 (3.03) 95 (94.05)
Gentamicin 84 (75.67) 61 (68.53) 27 (24.32)
Erythromycin 69 (64.48) 42 (45.16) 38 (35.51)
Ciprofloxacin 107 (100) 93 (100) 00 (0)

 Corpus Overall Overall
Antibiotics No res (%) Sus (%) Res (%)

Clarithromycin 21 (22.34) 160 (80) 40 (20)
Tetracycline 30 (31.25) 135 (67.5) 65 (32.5)
Amoxicillin 3 (3.15) 195 (97.5) 5 (2.5)
Metronidazole 96 (96.96) 9 (4.5) 191 (95.5)
Gentamicin 28 (31.46) 145 (72.5) 55 (27.5)
Erythromycin 51 (54.83) 111 (55.5) 89 (44.5)
Ciprofloxacin 00 (0) 200 (100) 00 (0)

Antibiotics [micro]g/ml

Clarithromycin 0.125 - 1.0
Tetracycline 1.25 - 2.0
Amoxicillin 2.5 - 5.0
Metronidazole >10
Gentamicin 5 - 8.0
Erythromycin 2.5 - 5.0
Ciprofloxacin 0.0625 - 1.0

Res = resistance; Sus = susceptibility. Zone diameter breakpoints for
clarithromycin testing were <14 mm resistance (R) and [greater than or
equal to] 14 susceptible (S); for tetracycline and amoxicillin <16 mm
(R) and [greater than or equal to] 16 (S); for metronidazole testing
<10 mm (R) and [greater than or equal to] 10 (S); for ciprofloxacin
<17 mm resistance (R) and [greater than or equal to] 17 mm susceptible
(S); for gentamicin <15 mm resistance (R) and [greater than or equal
to] 15 susceptible (S); and for erythromycin testing <19 mm resistance
(R) and [greater than or equal to] 20 susceptible (S). (1,13)
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Title Annotation:Original Articles
Author:Tanih, Nicoline F.; Okeleye, Benjamin I.; Naidoo, Nathan; Clarke, Anna M.; Mkwetshana, Noxolo; Green
Publication:South African Medical Journal
Article Type:Report
Geographic Code:6SOUT
Date:Jan 1, 2010
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