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Magnetic [Fe.sub.3][O.sub.4]/Ag hybrid nanoparticles as surface-enhanced Raman scattering substrate for trace analysis of furazolidone in fish feeds.

1. Introduction

Surface-enhanced Raman scattering or surface-enhanced Raman spectroscopy (SERS) has emerged as an important tool for trace analysis in many fields, such as chemistry, biology, environment, and food due to its ultrahigh sensitivity and potential rapidity or nondestructivity [1-3]. Raman enhancement effect is mainly attributed to the surface plasmon resonance (SPR) very close to the surface of nanostructured noble metal substrate [4, 5]. Enormous SPR is often present at the gaps between microstructures, resulting in the so-called hot spots. In general, nanoparticles suspended in a well-dispersed system containing target molecules lead to rather weak SERS signals, while aggregated NPs result in better enhancement effect since more interparticle narrow gaps could be provided on the surface of aggregated colloids [6]. The use of magnetic oxides/noble metal hybrid NPs makes it possible to assemble NPs in a relative orderly aggregated pattern with the assistance of an external magnetic field and has shown great advantages as SERS substrates [7-9]. For instance, Hu and Sun reported a robust strategy to construct Au coated multilayer magnetic nanoparticles with tunable hot spots by controlling the seed-mediated plating process, resulting in ultrasensitive SERS detection for rhodamine 6G as low as femtomolar [7]. Bao et al. fabricated Ag-[Fe.sub.3][O.sub.4] NPs and maximized SERS sensitivity through changing the size and density of Ag particles. The optimized NPs could be used to detect rhodamine 6G at a concentration as low as 10-12 [8]. Wheeler et al. demonstrated that magnetically induced aggregation of the [Fe.sub.3][O.sub.4]-Au core-shell NPs enhanced SERS activity for ~7 times compared to nonmagnetically aggregated [Fe.sub.3][O.sub.4]-Au NPs [9].

SERS is considered as first layer effect, and the analyte molecules must be very close to or adsorbed onto substrates to achieve significant enhancement effect. The surface of a SERS substrate must be clean so that targeted molecules could be adsorbed on to the surface. However, precursors and reducing agents used during NPs synthesis are often present on the surface of NPs, interfering with effective adsorption of analytes molecules [10]. The precursors and reducing agents used for the synthesis of noble metal-coated [Fe.sub.3][O.sub.4] NPs could be washed off under an externally exerted magnetic force, resulting in clean substrate surfaces. Therefore, magnetic noble metal NPs often lead to higher enhancement effects than nonmagnetic NPs [11].

The objective of this study was to synthesize [Fe.sub.3][O.sub.4]/Ag hybrid NPs suitable for analysis of trace amounts of furazolidone in animal feeds. Furazolidone is a nitrofuran antibiotic often applied illegally as animal or fish feeds, although it is prohibited by many countries due to its carcinogenicity and other adverse health effects [12]. There are very few studies published on the application of SERS for analysis of nitrofuran antibiotics, and these studies mainly used standard solutions instead of actual samples [13-15]. To the best of our knowledge, there is no report on applying SERS to analyze nitrofuran antibiotics in animal or fish feeds. Complex sample systems (such as fish feeds) contain numerous nontargeted compounds that may interfere with and sometimes even make it impossible for the SERS analysis of targeted molecules. In this study, easy-to-prepare superparamagnetic [Fe.sub.3][O.sub.4]/Ag hybrid NPs were synthesized and applied for trace detection of furazolidone in fish feeds with pronounced SERS activity, which helps understand the matrix effects on the SERS analysis and serves as a basis for further studies on rapid SERS method development for nitrofuran antibiotics in complex animal/fish feed systems.

2. Materials and Methods

2.1. Reagents. Analytical grade ferric chloride (Fe[Cl.sub.3] x 6[H.sub.2]O), ferrous chloride (Fe[Cl.sub.2] x 4[H.sub.2]O), ethanol, sodium hydroxide, ammonia solution (30%), dichloromethane, anhydrous sodium sulfate, alumina, acetonitrile (HPLC grade), and hexane (HPLC grade) were purchased from Sinopharm Chemical Reagent Co., Ltd. (number 52 Ningbo Road, Shanghai, China); silver nitrate ([greater than or equal to] 99%), hydroxylamine hydrochloride ([greater than or equal to] 99%), 3-aminopropyltrimethoxysilane (APTMS, 97%), and furazolidone (ACS grade) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All aqueous solutions were prepared using ultrapure water (18.2 M[OMEGA] cm).

2.2. Synthesis of [Fe.sub.3][O.sub.4]/Ag Hybrid NPs. The coprecipitation method was used to synthesize [Fe.sub.3][O.sub.4] NPs, in which ferric and ferrous irons (molar ratio 2 :1) were coprecipitated to [Fe.sub.3][O.sub.4] in alkaline solutions at room temperature and the resultants were treated under hydrothermal conditions [16, 17]. The as-synthesized iron oxide particles were hydrophilic, which could be easily dispersed in AgN[O.sub.3] solutions for later Ag particles growing and aggregating with [Fe.sub.3][O.sub.4] NPs. The reaction was performed under oxygen-free environment by passing N2 gas. In brief, FeCl3-6[H.sub.2]O (5.40 g) and Fe[Cl.sub.2] x 4[H.sub.2]O (1.99 g) were thoroughly mixed in 100 mL water. Next, N[H.sub.3] x [H.sub.2]O (30%) was added with vigorous stirring until the pH was 10, and the mixture was continuously stirred for 10 minutes at room temperature. The mixture was then heated at 80[degrees]C for 30 min. Following this, the mixture was washed under magnetic field with ethanol and water three times for each and then dried in vacuum oven at 70[degrees]C for 2 hours.

To be able to attach Ag particles and [Fe.sub.3][O.sub.4] NPs, APTMS was used as a linker between [Fe.sub.3][O.sub.4] and Ag NPs [18, 19]. To obtain APTMS-modified [Fe.sub.3][O.sub.4] NPs, [Fe.sub.3][O.sub.4] NPs (0.25 g) were added into ethanol (100 mL) and sonicated for 40 minutes. Next, APTMS (1mL) was added to the mixture followed by stirring for 6 hours. Then the mixture was rinsed four times with ethanol under an external magnetic field and dried in a vacuum oven at 70[degrees]C for 2 hours.

Four different types of hybrid NPs were constructed by aggregation of APTMS-modified [Fe.sub.3][O.sub.4] NPs and Ag particles through adjusting the molar ratio of AgN[O.sub.3] and [Fe.sub.3][O.sub.4] NPs (2:1, 3:1, 4:1, 5:1). The amount of [Fe.sub.3][O.sub.4] NPs was fixed (0.05 g, 0.22 mmol), and four different levels of AgN[O.sub.3] solutions (0.07 g, 0.44 mmol; 0.11 g, 0.66 mmol; 0.15 g, 0.88 mmol; 0.19 g, 1.10 mmol) were mixed with [Fe.sub.3][O.sub.4] NPs and sonicated for 40 minutes, respectively. Then, freshly prepared reductant mixtures consisting of 50 mL 0.1 M NaOH and 45 mL 0.06 M hydroxylamine hydrochloride were added dropwise and mixed for 45 minutes. Finally, the NPs were washed with water under an external magnetic field until the supernatant was clear. The four [Fe.sub.3][O.sub.4]/Ag hybrid NPs samples were labeled as FA-1, FA-2, FA-3, and FA-4, respectively. Figure 1 is a summary of the procedure for synthesis of [Fe.sub.3][O.sub.4]/Ag hybrid NPs and later substrate preparation.

2.3. Characterization of Composite Nanoparticles. General shapes and sizes of [Fe.sub.3][O.sub.4] NPs and [Fe.sub.3][O.sub.4]/Ag hybrid NPs were characterized by a JEM-2100F transmission electron microscopy (TEM; JEOL Ltd., Tokyo, Japan). The samples for TEM characteristics were prepared by making a carbon-coated copper grid soaked in as-prepared NPs for 30 minutes before being dried at room temperature. Scanning TEM equipped with a high angle annular dark field detector (STEM-HAADF) and energy dispersive spectroscopy (EDS) elemental mapping was used to further understand the structure and component of particles. The magnetic properties were investigated by using a Quantum Design superconducting quantum interference device (SQUID) magnetometer (MPMS SQUID VSM, Quantum Design Ltd., USA) at room temperature.

2.4. Preparation of Fish Feed Samples. Fish feeds from the College of Fisheries and Life Science in Shanghai Ocean University were spiked with furazolidone at a series of concentrations (200,500,1000, and 5000 ng m[L.sup.-1]). Furazolidone was then extracted and purified from fish feeds based upon the method of Hu et al. [20]. In brief, furazolidone was extracted with dichloromethane three times and purified through anhydrous sodium sulfate and alumina column successively. The column was a syringe (10 mL) containing a filtration membrane (0.45 [micro]m), 4.0 g of anhydrous sodium sulfate, and 2.0 g of alumina. The eluate was evaporated to almost dryness at 50[degrees]C in water base with a rotary evaporator (R206B, Shanghai SENCO Technology Ltd., Shanghai, China), dissolved into 1.0 mL of acetonitrile/water (8 : 2) and 1.0 mL of hexane, and then centrifuged. The supernatant hexane was discarded, followed by adding 1.0 mL of hexane to defat the matrix. Finally, the subnatant was collected and used for SERS analysis.

2.5. SERS Analysis. A series of furazolidone standard solutions (1000, 500,100, 50, 40, and 20 ngm[L.sup.-1]) were prepared in acetonitrile. For SERS measurement, 10 [micro]L of [Fe.sub.3][O.sub.4]/Ag NPs was first added onto a glass slide on top of the magnet, and furazolidone standard solution was added on top of NPs droplets. After the substrates were dried under ambient conditions, 20 spectra were collected at different positions of the substrates and the average of these spectra was used as the final spectrum for the sample. To compare the enhancement effect of four different [Fe.sub.3][O.sub.4]/Ag hybrid NPs, SERS spectra of furazolidone standards (100 and 500 ng m[L.sup.-1]) were collected with each type of NPs, respectively, and the experiments were repeated five times. In addition, SERS enhancement effect without the use of magnetic field was examined by removing the magnetic field during [Fe.sub.3][O.sub.4]/Ag NPs substrate preparation for the SERS measurement.

The SERS measurement of [Fe.sub.3][O.sub.4]/Ag NPs with different amounts of silver was carried out on a Nicolet DXR microscopy Raman spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). A 780 nm HP He-Ne laser source with 20 mW laser power and a 20x objective was used for spectral collection. All spectra were recorded with a 2-second exposure time for each scan, and the final spectra were the sum of two scans.

3. Results and Discussion

3.1. Characterization of [Fe.sub.3][O.sub.4]/Ag Hybrid NPs. The TEM images of [Fe.sub.3][O.sub.4] NPs are exhibited in Figure 2(a), and their average diameter was 12 [+ or -] 1 nm calculated from 100 particles. The [Fe.sub.3][O.sub.4] NPs had relative uniform and narrow size distribution (standard deviation 9% < 10%[21]). Figure 2(b) shows TEM images of representative [Fe.sub.3][O.sub.4]/Ag hybrid NPs (FA-3) synthesized in this study, in which the lighter small particles are [Fe.sub.3][O.sub.4], while the darker large particles are [Fe.sub.3][O.sub.4]/Ag hybrid NPs (FA-3). The figure indicates that APTMS-modified [Fe.sub.3][O.sub.4] NPs are successfully aggregated with Ag particles. Through the condensation reaction between -OH groups on the surface of [Fe.sub.3][O.sub.4] NPs and APTMS, N[H.sub.2] groups are then decorated on the [Fe.sub.3][O.sub.4] NPs, which allows for coordination and static binding of Ag particles with negatively charged surface [18]. Figure 2(c) shows statistical histogram of diameters and relative standard deviation (RSD) of four [Fe.sub.3][O.sub.4]/Ag hybrid NPs (n = 100). The particle sizes were 58 [+ or -] 7 nm, 63 [+ or -] 8 nm, 73 [+ or -] 9 nm, and 106 [+ or -] 14 nm, respectively, increasing gradually with an increase of molar concentration of Ag+ ions used for hybrid NPs aggregation. The size distributions of four samples were all less than 15%, indicating relatively uniform particle size. Figure 2(d) is the EDS spectrum of [Fe.sub.3][O.sub.4]/Ag NPs, which shows the peaks corresponding to Ag and Fe elements. The presence of Cu elements was due to the carbon-coated copper grid used as the holder for nanoparticles. The STEM-HAADF image and element mapping images for [Fe.sub.3][O.sub.4]/Ag NPs indicate that [Fe.sub.3][O.sub.4]/Ag hybrid NPs are aggregates of [Fe.sub.3][O.sub.4] and Ag particles and contain denser Ag particles than [Fe.sub.3][O.sub.4] particles (Figures 3(a)-3(d)).

Figure 4 exhibits the magnetization curves of dried [Fe.sub.3][O.sub.4] NPs and two [Fe.sub.3][O.sub.4]/Ag hybrid NPs(FA-3 and FA-4) measured at room temperature. The saturated magnetization (SM) value of [Fe.sub.3][O.sub.4] NPs was 83.0 emu x [g.sup.-1], which was higher than 78.0 emu x [g.sup.-1] reported by Kim et al. [22]and 53.7 emu x [g.sup.-1] by Bao et al. [8]. The negligible coercivity and reversible hysteresis behavior indicated the superparamagnetic nature of [Fe.sub.3][O.sub.4] NPs, which is common for magnetite smaller than 30 nm in diameter [23]. The SM values of FA3 and FA-4 were 46.7, 36.5 emu x [g.sup.-1]1, respectively. The SM values decreased with an increase of Ag ratio in the hybrid NPs due to the diamagnetic contribution of Ag particles [22]. The magnetic response for all four hybrid NPs is strong enough to make it easy to rinse the NPs and to conduct magnetically induced aggregation in a magnetic field. The excellent magnetic property of hybrid NPs also allows separating the particles over a short time (less than 30 seconds) in an external magnetic field (Figures 4(D) and 4(E)).

3.2. Effect of Magnetic Self-Assembly Method on SERS Sensitivity. In order to understand the effect of the ratio of Ag particles on the SERS sensitivity, four different [Fe.sub.3][O.sub.4]/Ag hybrid NPs were used as substrates to collect the SERS spectra of furazolidone standards 100 and 500 ngm[L.sup.-1], respectively Figure 5(a) displayed the representative SERS spectra of 500ngm[L.sup.-1] furazolidone acquired with the use of four hybrid NPs substrates. All four spectra consist of characteristic peaks at 1561, 1490, 1348, and 1014 [cm.sup.-1] associated with furan ring stretching vibrations. The peak at 1606 [cm.sup.-1] is assigned to C=N stretching vibration in the plane, while medium bands at 1243, 960, and 807 [cm.sup.-1] are attributed to C-H bending vibrations and C-C stretching vibrations [13]. Notably, the SERS signals from FA-3 were much stronger than the others. Similar trends were observed for SERS spectra of 100 ng m[L.sup.-1] furazolidone. Using the characteristic peak at 1348 [cm.sup.-1] as an example (Figure 5(b)), its Raman intensity increased with an increase in hybrid particle size until reaching 73 [+ or -] 9 nm but then decreased with a further increase of particle size. Controlling the amount of Ag particles aggregated with modified-[Fe.sub.3][O.sub.4] NPs through adjusting the molar ratio of Ag+ ions and [Fe.sub.3][O.sub.4] NPs makes the SPR tunable and eventually optimizes Raman enhancement effect for furazolidone. In addition, although it is often a challenge to obtain reproducible SERS spectra, by using the average of spectra collected at 20 different positions of a substrate, we can overcome this problem. As shown in Figure 5(b) for the Raman intensity of 500ngm[L.sup.-1] and 100ngm[L.sup.-1], with furazolidone at the characteristic peak of 1348 [cm.sup.-1], the error bars calculated from quintuplicate analyses indicated very small standard deviation for spectra collected independently, implying that repeatable spectra could be obtained with [Fe.sub.3][O.sub.4]/Ag hybrid NPs.

We also attempted to analyze furazolidone with Ag NPs varying in particle size (34-67 nm) synthesized using the method of Leopold and Lendl [24], butveryweakSERS enhancement was obtained. This was probably due to the interference of remaining reductive agent on the surface of Ag NPs that affected the effective adsorption of furazolidone. On the contrary, the surface of [Fe.sub.3][O.sub.4]/Ag hybrid NPs was clean because reductive agent and other nontargeted compounds could be removed by washing with copious amounts of water under external magnetic field before the NPs were used for SERS analysis.

Using a magnetically directed self-assembly method is a simple and effective way to condense NPs on a glass slide used as holder for substrate and analyte molecules to increase SERS signals [6]. Figure 6 shows the SERS spectra of furazolidone obtained using 73 [+ or -] 9 nm [Fe.sub.3][O.sub.4]/Ag hybrid NPs assembled onto a glass slide with and without an external magnetic field. The spectra obtained with substrates assembled under magnetic field displayed stronger SERS signals, with about twice to fourfold increase in enhancement effect compared to those without magnetic field. NPs assembled in a more orderly arrangement by the magnetic force led to the formation of more relatively homogeneous SERS hot spots and therefore better enhancement effect [6].

The FA-3 [Fe.sub.3][O.sub.4]/Ag hybrid NPs were used as SERS substrate to further acquire spectra of furazolidone with different concentrations, as shown in Figure 7(a), characteristic peaks of furazolidone were clearly visible at concentration as low as 40 ng m[L.sup.-1], and the intensity of SERS signals increased as the concentration changed from 40 to 1000 ng m[L.sup.-1]. This indicated that [Fe.sub.3][O.sub.4]/Ag hybrid NPs exhibited significant Raman enhancement effect as SERS substrate for furazolidone detection.

3.3. SERS Analysis of Furazolidone in Spiked Fish Feed Samples. The SERS-optimized spectra (acquired with FA-3 hybrid NPs) of extracts from spiked fish feeds consist of main characteristic peaks of furazolidone consistent with those of the standards, although some extra bands (such as the ones at around 1050 and 1447 [cm.sup.-1]) are observed in the spectra of extracts (Figure 7(b)). These extra bands may be due to some residual compounds, such as amino acids and pigments naturally present in fish feeds. Due to the high sensitivity of SERS method, some trace amounts of nontargeted compounds from fish feed may produce SERS signals. In addition, nontargeted compounds in the extracts may interfere with effective adsorption of furazolidone molecules onto the NPs and reduce the sensitivity of SERS method. As shown in Figure 7(b), the intensity of furazolidone characteristic peaks for extracts was weaker than that for standards. The lowest concentration with discernible characteristic peaks of furazolidone was 500 ng[g.sup.-1] for extracts, which is much higher than that of 40 ng m[L.sup.-1] for standards. However, the sensitivity of the SERS method for furazolidone in fish feed is comparable to a reported HPLC-UV method (detection limit, 1 [micro]g [g.sup.-1]) [25] and a LC-MS method for animal feeds (detection limit, 100 ng [g.sup.-1])[26]. In addition, the sensitivity of the SERS method could meet the requirement for analysis of furazolidone in animal and fish feeds, which are often added with the antibiotics ranging from 8 [micro]g[g.sup.-1] to 400 [micro]g[g.sup.-1] [27].

4. Conclusions

Superparamagnetic [Fe.sub.3][O.sub.4] NPs were synthesized and modified using APTMS to add N[H.sub.2] groups on their surfaces and then aggregated with Ag particles to form [Fe.sub.3][O.sub.4]/Ag hybrid NPs. The [Fe.sub.3][O.sub.4]/Ag hybrid NPs exhibited tunable plasmonic properties of SERS by adjusting the amount of Ag particles aggregated with [Fe.sub.3][O.sub.4] NPs through modulating molar ratios of the [Ag.sup.+] and [Fe.sub.3][O.sub.4]. The use of 73 [+ or -] 9 nm [Fe.sub.3][O.sub.4]/Ag hybrid NPs assembled onto a glass slide under an external magnetic field as SERS substrate could detect furazolidone standards and furazolidone in fish feeds at the levels as low as 40ngm[L.sup.-1] and 500ng[g.sup.-1], respectively. To improve the applicability of the SERS analysis method, simplifying sample preparation and development of selective SERS substrates are much needed for future research.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.


This research was supported by Innovation Program of Shanghai Municipal Education Commission (14YZ123), funding program for outstanding dissertations (B1-9600-120001-5), and the National Natural Science Foundation of China (61250002 and 31250006).


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Wansong Yu, Yiqun Huang, Lu Pei, Yuxia Fan, Xiaohui Wang, and Keqiang Lai

College of Food Science and Technology, Shanghai Ocean University, 999 HuchengHuan Road, Lingang New City, Shanghai 201306, China

Correspondence should be addressed to Keqiang Lai;

Received 7 May 2014; Revised 6 July 2014; Accepted 8 July 2014; Published 23 July 2014

Academic Editor: Bin Zhang
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Title Annotation:Research Article
Author:Yu, Wansong; Huang, Yiqun; Pei, Lu; Fan, Yuxia; Wang, Xiaohui; Lai, Keqiang
Publication:Journal of Nanomaterials
Date:Jan 1, 2014
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