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Liquid chromatography tandem mass spectrometry identification of proanthocyanidins in rat plasma after oral administration of grape seed extract.


Proanthocyanidin rich plant extracts derived from grape seed extract (GSE), hawthorn and cranberry are on markets for their preventive effects against cardiovascular diseases and uroinfections in woman. However, the importance of these health beneficial effects of these botanicals remains elusive due to incomplete understanding of uptake, metabolism and bioavailability of proanthocyanidins in vivo. In the present study rats were given GSE orally (300 mg/kg, twice a day) and blood and urine were collected over a 24 h period. Monomeric catechins and their methylated metabolites, and proanthocyanidins up to trimers were detected in blood samples treated with GSE using LC-MS/MS operating in the multiple reaction monitoring (MRM) mode. A new tetramethylated metabolite of dimeric proanthocyanidin (m/z 633) in GSE-treated urine was tentatively identified. Using LC-MS/MS, ( + )-catechin and (--)-epicatechin were identified in the brain conclusively. These data suggested that GSE catechins cross the blood brain barrier and may be responsible for the neuroprotective effects of GSE.

[C] 2008 Elsevier GmbH. All rights reserved.

Keywords: Grape seed proanthocyanidins; Metabolism; LC-MS/MS; Brain


Grape seeds, one of the richest sources of polyphenols, have potent antioxidant activity. Extracts prepared from the grape seeds contain a rich mixture of monomeric flavan-3-ols, phenolic acids and oligomeric proanthocyanidins. There has been a great surge in commercialization of botanical extracts containing proanthocyanidins. In Europe some standardized plant extracts (e.g. cranberry- or hawthorn-extracts) are on the market as conventional drugs which are used because of their content of oligomeric proanthocyanidins for the treatment of recurrent uroinfections in woman and cardiovascular diseases grade I and II, respectively.

Proanthocyanidins are mixtures of dimers and higher oligomers of monomeric flavan-3-ol units linked mainly by a C4-C8 bond and, to a lesser extent, a C4-C6 bond (both are called B-type proanthocyanidins) (Fig. 1) (da Silva et al. 1991; Boukharat 1988; Prieur et al. 1994). The proanthocyanidins (PAC) of hawthorn-extracts (Crataegus spez.) belong to the B-type with only C-C-links between their (epi)catechin monomer units, whereas the major proanthocyanidins of cranberry (Vaccinium macrocarpum) as typical A-type PACs possess also C--O--C-linkages between their monomer units. The latter type only seems to be predestinated for its antihesion and antiuroinfection activity (Howell et al. 1998; Nowack and Schmitt 2008). Emerging evidence has indicated that grape seed proanthocyanidins have cardioprotective effects against reperfusion-induced injury via their ability to decrease, directly or indirectly, free radicals in myocardium (Pataki et al. 2002). It has also been demonstrated that consumption of GSE decreases the incidence of cataracts in the eyes of hereditary cataractous (ICR/f) rats (Yamakoshi et al. 2002). In addition, studies involving grape seed extract (GSE) found that chronic exposure to GSE modulates expression of specific mouse brain proteins that are either linked to Alzheimer's Disease or neuron-degeneration (Deshane et al. 2004).


Given the increasing significance of a potential health beneficial role of GSE proanthocyanidins in human health, there is a need for a fuller understanding of their absorption, metabolism and excretion. (Epi)catechins are absorbed from the intestines in humans and animals, appearing in plasma and urine primarily as glucuroni-dated, methylated and sulphated metabolites following the ingestion of diet containing monomeric catechins and proanthocyanidis such as chocolate, black and green tea, and red wine (Yang et al. 1998; Rein et al. 2000; Piskula and Terao 1998; Donovan et al. 1999). However, the metabolic fate of proanthocyanidins is still elusive as there is conflicting evidence on the absorption and metabolism of the oligomeric and polymeric flavan-3-ols in humans and animals. For example, Koga et al. (1999) reported the presence of ( + )-catechin and (--)-epicatechin and an absence of dimers in the plasma of rats following ingestion of a GSE. However, studies by Holt et al. (2002) demonstrated the presence of dimeric procyanidins in human plasma as early as 30 min after the consumption of a flavanol-rich food such as cocoa. There is evidence in support of absorption of monomeric catechins and proanthocyanidins through the human intestinal caco-2 epithelial cells (Deprez et al. 2001; Faria et al. 2006). Another study by Tsang et al. (2005) reported the absorption and metabolism of catechin and proanthocyanidins up to trimers in urine following the oral intake of GSE. However, this study was inconclusive in establishing the accessibility of catechin in the brain. It is, therefore, critical to understand the metabolic fate of proanthocyanidins in an experimental animal model. In this study, we describe identification of major metabolites of monomeric flavan-3-ols and proanthocyanidins after oral administration of GSE and their accessibility to the brain by LC-MS/MS methods.

Materials and methods

Chemicals and reagents

( + )-Catechin (98% purity), (-)-epicatechin (98% purity) and apigenin were purchased from Indofine chemical company Inc. (Hillsborough, NJ, USA). All other HPLC solvents and reagents were purchased from Fisher (Norcross, GA) and were of HPLC grade. A powdered GSE preparation was provided by Kikkoman Corp. (Chiba, Japan) and stored at 4 [degrees]C in light-tight containers until used. The composition of GSE was previously described to consist of > 95% flavanols, of which 86% were proanthocyanidins (Yamaguchi et al. 1999).

GSE administration and sample collection

Male Sprague-Dawley rats (SD, n = 8, body weight = 298-340 g, 12 weeks of age) were obtained from Harlan Sprague-Dawley Inc., Indianapolis, IN. They were kept in a controlled environment at 23 [degrees]C and 55% relative humidity under a 12 h dark-light cycle, with free access to soy-free custom diet TD86369 (Harlan Teklad, Wisconsin, MD) and tap water for 1 week. All experimental procedures were conducted in accordance with Institutional Animal Care and Use Committee of the University of Alabama, Birmingham, and National Institutes of Health guidelines. After overnight fasting, GSE in aqueous solution was gavaged (300mg/kg, twice a day) to the rats. Blood and brain were collected after 4h of GSE administration on the 3rd day. Urine samples were collected using metabolic cages over 16h after each GSE treatment. Rats were euthanized by cervical dislocation following isoflurane anesthesia and blood was collected via heart puncture using heparin as anticoagulant. Plasma was isolated by centrifugation of the blood at 3000g for 5 min and stored at -20 [degrees]C. The brain was dissected after perfusion with ice-cold normal saline for 10 min and immediately frozen in liquid nitrogen.

For the analysis of plasma and urinary metabolites, samples were hydrolyzed enzymatically with sulfatase extracted from Helix pomatia (Sigma, St, Louis, MO) to hydrolyze glucuronide and sulfate conjugates. In brief, phenolphthalein glucuronide, 4-methylumbelliferyl sulfate, and apigenin were added as internal standards to each plasma and urine sample before hydrolysis with 400 U of [beta]-glucuronidase and aryl sulfatase in 150 mmol/l ammonium acetate buffer, pH 5, for 16 h at 37 [degrees]C. The samples were extracted with ethyl acetate, then evaporated to dryness, and finally reconstituted in 80% aqueous methanol.

Two brain samples were combined into one (3.4-3.7 g), minced and added to methanol (6.5 ml) containing 0.5% acetic acid. The tissue was homogenized with a Tissue Tearor homogenizer (Biospace Product Inc., Racine, WI). The samples were vortex mixed and tumbled for 2 h at room temperature. The homogenate was centrifuged at 3000g for 10 min and the supernatant was removed and dried under air and reconstituted in 80% aqueous methanol (200 [micro]l). The reconstituted solutions were transferred to HPLC auto sampler vials and injected into the mass spectrometer to analyze the samples.

MALDI-TOF MS analysis

Reflectron mode MALDI-TOF mass spectral data were acquired on an Applied Biosystems Inc. (Foster City, CA) Voyager DE-Pro instrument equipped with delayed extraction and a nitrogen laser at 337 nm. The positive reflectron mode spectra collected using an accelerating voltage of 20 kV and a delay time of 100 ns; 300 laser shots were accumulated and averaged. The spectra were calibrated using an external calibration of a bradykinin peptide at m/z 1060.22. The laser intensity was set at 2500 with mass range of 500-3000 Da.

Liquid chromatography-mass spectrometry

LC-MS/MS analyses of rat urine, plasma and brain samples were performed using a system consisting of a model SIL-HT refrigerated Shimadzu auto sampler, an HPLC instrument (Shimadzu Scientific Instruments, Inc. Columbia, MD), and an API 4000 mass spectrometer (Applied Biosystems/MDS Sciex, Concord, On tario, Canada). Chromatography was carried out on a reversed-phase Phenomenex Fusion C18 column (150 x 2.0 mm i.d.) with the mobile phase consisted solvent A (0.1% formic acid) and solvent B (methanol containing 0.1% formic acid). A 10 min gradient was established running from 10% to 100% B over the first 5 min and the system was returned to the initial 10% B at 6 min.

The column effluent was introduced into the mass spectrometer using electrospray ionization (ESI) in the negative and positive ion modes. Nitrogen was used as nebulizer, and curtain gas. The nebulizer current and temperature were 5 A and 250 [degrees]C, respectively. The collision gas ([N.sub.2]) was set at high and collision energy was 30 eV with electron multiplier voltage (1900 V). The following mass transitions were used for MRM analysis of (epi)catechin m/z 291/139 (positive ion mode) and 289/137 (negative ion mode), methyl (epi)catechin m/z 305/139 and 305/137, proanthocyanidin dimers m/z 579/ 289 and 579/291, proanthocyanidin trimers m/z 867/579 in positive ion mode. The LC-MS-MS system was controlled by BioAnalyst 1.4.1 software.

Chromatographic separation of catechins and proanthocyanidins in the methanolic extract of grape seed was performed using a 250 x 2.0 mm i.d. (4 [micro]m) Synergy Hydro-RP 80R (Phenomenex, Torrance, CA) with a 15min gradient running from 10% to 60% B in the first 7min and increased from 60% to 100% over the next 2min and then returned to 10% from 9 to 10min. LC-MS/MS conditions were similar as explained above. For quantification of (+)-catechin and (-)-epicatechin in GSE, a methanolic solution of GSE (164 [micro]g/ml) was prepared in 80% methanol in water, filtered, and transferred to auto samplers for LC-MS/MS analysis. GSE solution, (+ )-catechin and (-)-epicatechin standards were injected (10 [micro]1) onto the LC-MS/MS system. The quantification of (+)-catechin and (-)-epicatechin in GSE was based on a calibration curve over the concentration range 0.01-10 [micro]M. The assay method for quantification was demonstrated to be very sensitive and specific, with a correlation coefficient of 0.99.


Analysis of grape seed extract

ESI-MS analysis of GSE in the negative ion mode indicated the extract was a complex mixture containing monomeric catechin and epicatechin (m/z 289.1), and proanthocyanidin dimers (m/z 577.5), trimers (m/z 865.5), tetramers (m/z 1153.8) and their gallate esters such as (epi)catechin gallate (m/z 441.2) and gallate of trimeric proanthocyanidins (m/z 1017.1) (Fig. 2A). A number of reversed-phase LC columns were screened in order to obtain the best separation of monomeric catechins and proanthocyanidis. A synergi Hydro-RP column (C18 with polar end capping) provided excellent chromatographic separation and peak shape of monom-ric catechins and proanthocyanidins. Fig. 2B illustrates typical chromatograms for GSE that demonstrate the base line separation of (+)-catechin ([t.sub.R] 9.45 min) and (-)-epicatechin ([t.sub.R] 10.39 min). The mean amounts of (+)-catechin and (-)-epicatechin in GSE were 65.95 and 27.40 [micro]g/mg, respectively.


Since the proanthocyanidins are formed from the condensation of monomeric units of catechin or epicatechin, they differ in their position and configuration of their monomeric linkage. As can be seen in Fig. 2C, a MRM ion chromatogram of m/z 577/289 in negative ion mode showed at least five well resolved peaks corresponding to different dimers. They could be dimers B1-B5, as previously reported for GSE (Sun et al. 1999). In order to determine the composition of oligomeric and polymeric proanthocyanidins, analysis using MALDI-TOF/MS was also carried out.

MALDI-TOF MS analysis

Matrix-assisted-laser-desorption-ionization-time-of-flight (MALDI-TOF) analysis is a facile way to determine the presence of molecules of intermediate molecular weight with high accuracy. Reflectron mode MALDI-TOF MS analysis was performed to identify oligomeric proanthocyanidins present in GSE. 2,4,6-Trihydroxyacetophenone (THAP) matrix was used as the MALDI matrix. MALDI-TOF MS spectra of GSE contained peaks with m/z values corresponding to an oligomeric series of catechin/epicatechin/gallo(epi)cate-chin units up to nonamers in the reflectron mode (Table 1). Ions were detected in the positive ion mode as sodium adducts [[M + Na].sup.+] . Galloylated proanthocyanidins were identified with the mass shifts [[M + 152x + Na].sup.+] where x represents the number of gallic acid esters (Table 1).
Table 1. Proanthocyanidins (PA) detected in GSE by MALDI-TOFMS in
reflection mode

PA        Number of galloyl unit  Observed [[M + Na].sup.+]

Dimer               0                       601
                    1                       753

Trimer              0                       889
                    1                       1041
                    2                       1193
                    3                       1345

Tetramer            0                       1177
                    1                       1329
                    2                       1481

Pentamer            0                       1465.5 (1466 calculated)
                    1                       1618

Hexamer             0                       1754
                    1                       1907

Heptamer            0                       2041.7 (2042 calculated)
Octamer             0                       2330
Nonamer             0                       2619

Identification of flavan-3-ol and proanthocyanidin metabolites

Urinary metabolites

LC-MS/MS with ESI turbo ionspray analysis of rat urine after enzymatic hydrolysis was performed using multiple MS/MS experiments (MRM and MS/MS) in negative and positive ion modes to identify the flavan-3-ol and proanthocyanidin metabolites. MRM analysis with the mass transitions m/z 291/139 (catechin or epicatechin), 305/139 (methyl catechin or epicatechin), 579/291 (dimers), 579/289 (dimers) and 867/579 (tri-mers) indicated the presence of monomeric catechin or epicatechin, methylated catechin or epicatechin, and proanthocyanidin dimers and proanthocyanidin trimers in urine collected over 16 h after GSE treatment. Identity of monomeric catechins (catechin and epicatechin) was further confirmed by comparison with product ions and retention time ([t.sub.R]) of standards in LC-MS/MS experiments (data not shown).

Proanthocyanidins were only tentatively identified based on extensive interpretation of their product ion spectrum and previously published references (Tsang et al. 2005; Yamaguchi et al. 1999). For the identification of proanthocyanidins, we compared the product ion profile of metabolites with the proanthocyanidins of GSE administered to the rats. As can be seen in Fig. 3A, there are at least four major peaks with a mass transition m/z 579/289 at [t.sub.R] 2.2, 6.0, 6.1 and 6.6 min in the MRM analysis, indicating the presence of proanthocyanidin dimers. Similarly, a MRM ion chromatogram with mass transition m/z 867/577 showed three peaks at [t.sub.R] 2.2, 5.0 and 6.1min (Fig. 3B), corresponding to isomers of proanthocyanidin trimers in the GSE-treated urine sample. Further identification of proanthocyanidin dimers was obtained from LC-MS/MS analysis of m/z 577 [[M-H].sup.-]. As anticipated, a number of peaks were observed in the LC-MS/MS analysis of m/z 577 (Fig. 4). The mass spectra resulting from MS/MS experiments of m/z 577 at [t.sub.R] 2.2 and 6.1 min in the negative ion mode show product ions at m/z 451 [(M-H-126).sup.-], 425 [(M-H-152).sup.-], 407 [(M-H-170).sup.-], 289, and 287 which are consistent with those of proanthocyanidin dimers previously reported in grape seed (Sun et al. 1999). MS/MS spectra again demonstrated that the relative abundance of major product ions (e.g. m/z 289 and 407) varies indicating that they represent different isomeric forms and that arise from variation in the positioning of monomeric flavan-3-ol units. At this point, chemical structures of individual proanthocyanidins could not be characterized precisely due to unavailability of authentic standards.



We also detected a m/z 633 ion which on fragmentation yielded the following product ions, m/z 329, 303, 289 and 163 (Fig. 5). The most obvious fragmentation pathway is the breakage of the inter-flavan bond of m/z 633 leading to the formation of m/z 317 which corresponds to dimethylated (epi)catechin (Fig. 6). This ion on further fragmentation generates m/z 303 (mono-methylated (epi)catechin) and (epi)catechin m/z 289. The product ion m/z 329 could be the result of heterocyclic ring fission followed by further cleavage of the C3-4 position of upper unit of (epi)catechin. A neutral loss of water (-18 Da) and methyl group from the product ion m/z 329 is suggested for the generation of product ion m/z 297. These pieces of information led us to propose that the ion m/z 633 corresponds to a dimeric structure made up of two dimethylated (epi)ca-techins as shown in Fig. 6.



Plasma metabolites

Flavan-3-ol, monomelic catechins (catechin and epica-techin), their monomethylated metabolites, and proantho-cyanidin dimers and pronathocyanidin trimers were detected using LC-MS/MS in the MRM mode. The plasma concentration of (+)-catechin was below the limit of quantification. Interestingly, proanthocyanidins up to trimers were detected in 4 h after the GSE-treated plasma samples. As can been seen in Fig. 7, two well separated peaks with mass transitions m/z 579/291, 579/289 and m/z 867/579, indicated the occurrence of proanthocyanidin dimers and proanthocyanidin trimers, respectively.


Identification and quantification of GSE metabolites in rat brain

Two brain samples were pooled, extracted (as described in the experimental section) and analyzed for GSE metabolites using LC-MS/MS. Interestingly, the MRM analysis of brain samples indicated the presence of catechin and epicatechin (Fig. 8A). The structures of catechin and epicatechin were further confirmed by comparing their product ion spectra with those of standards (Fig. 8B). ( + )-Catechin concentration was found to be approximately 53.16 ng/g wet tissue, in the final brain extract.



A previous study has shown that GSE is a rich source of monomeric (epi)catechins and oligomeric proantho-cyanidins. (Reed et al. 2005). In the present study, proanthocyanidins up to nonamers and substantial but undetermined high molecular weight polymeric proanthocyanidins were detected. One of the major questions has been whether the high molecular weight proanthocyanidins are bioavailable in detectable amounts (Donovan et al. 2002). It has been suggested that the effects of dietary proanthocyanidins are unlikely to be due to proanthocyanidins themselves or monomeric metabolites with the intact flavonoid-ring structure, as they do not exist at detectable concentrations in vivo. However, emerging evidence has shown many health benefits of GSE including neuroprotection (Feng et al. 2007; Deshane et al. 2004).

In the present investigation, the mean amounts of ( + )-catechin and (-)-epicatechin fed to each rat by gavage were 10.2 and 4.25 mg, respectively. In addition, the GSE contained a substantial amount of proanthocyanidins. The plasma concentration of catechin 4 h after GSE administration was below the limit of quantification. This observation is in accord with the previous report, which indicated that catechins level in human plasma reach maximum 2 h after ingestion and decrease substantially after 3 h (Masukawa et al. 2006). The concentration of methyl (epi)catechin could not be estimated due to lack of standards. Proanthocyanidins up to trimers were detected in the blood with high specificity using the LC-MS/MS method. Previous studies by Shoji et al. (2006) indicated absorption of proanthocyanidins from the digestive tract and their presence in the rat plasma after the treatment with the proanthocyanidin oligomeric fraction of apple. Similarly, Baba et al. (2002) and Sano et al. (2003) reported the detection of proanthocyanidin dimers in rat and human plasma using LC-MS. Although Tsang et al. (2005) reported proanthocyanidin dimer and trimer in GSE-treated urine, this is the first report identifying proanthocyanidins up to trimers in blood and urine after GSE administration in rats. Unavailability of pure proanthocyanidins, complexity caused by different polymerization pattern and the isomers for each oligomer represent some of the problems in quantification of grape proanthocyanidin metabolites.

The low plasma concentrations of catechins and detection of proanthocyanidins indicates that proanthocyanidins were not necessarily degraded into only monomeric metabolites 4 h after administration. The absorption of higher oligomeric proanthocyanidins cannot be ignored, since the LC-MS/MS method is not sensitive enough to detect proanthocyanidins larger than trimers when the concentration is very low in biological samples. A previous report that proanthocyanidins are degraded to compounds with lower molecular weights by the colonic microflora in vitro warrants further confirmation in vivo (Deprez et al. 2000). Studies by Kahle et al. (2007) have indicated that 90% of the consumed proanthocyanidins were recovered in the ileostomy effluent and therefore would reach the colon under physiologic circumstances.

LC-MS/MS analysis of the urine from rats treated with GSE indicated the presence of monomeric catechins, proanthocyanidin dimers and proanthocyanidin trimers. As can be seen in Fig. 3A, there were four major peaks with retention times of 2.2, 6.0, 6.1 and 6.7 min, indicating four isomers of proanthocyanidin dimers. These results are in agreement with previous reports, in which proanthocyanidin Bl, B2, B3 and B4 dimers were detected in urine after GSE treatment (Tsang et al. 2005).

A number of flavonoid metabolites containing ortho-dihydroxy (catechol) groups are reported to be substrates for catechol-O-methyltransferase (COMT) in vitro (Nagai et al. 2004). Several reports have shown the methylated metabolites of catechin, epicatechin and quercetin (all having catechol-like structure) (Donovan et al. 1999; Hong and Mitchell 2004). To the best of our knowledge, this is the first report identifying a new tetramethylated metabolite of proanthocyanidin dimer (m/z 633) by LC-MS/MS, although Garcia-Ramirez et al. (2006) identified a dimeric proanthocyanidin consisting of two dimethylated epicatechins linked by an ethyl bridge in plasma after oral administration of synthetic oligomeric proanthocyanidins.

Recent studies have suggested that GSE might exert neuroprotective properties, so it was necessary to investigate whether GSE components are accessible to the brain to exert these effects (Feng et al. 2007). In this study, monomeric catechin and epicatechin were shown to have crossed the blood brain barrier and gained entry to the brain in GSE-treated rats. Although El Mohsen et al. (2002) have claimed the presence of the epicatechin glucuronide and 3'-O-methyl epicatechin glucuronide in the rat brain following oral ingestion of epicatechin, more definitive analytical data were needed, particularly MS/MS product ions that truly correspond to glucur-onidated metabolites of epicatechin and methyl catechin. Other flavonoids such as naringenin and puerarin have been detected in rat brain samples (Peng et al. 1998; Prasain et al. 2004).

In our study, to ensure that the detected metabolites had actually crossed the brain endothelial cell barrier and passed to the brain, brain tissues from animals that were perfused through the heart using ice-cold saline were used. The results show the presence of unconjugated catechin and epicatechin in the brain tissue. Thus the catechins found in the brain were located intracellularly or interstitially and not intravascularly.

In conclusion, monomeric catechins and proantho-cyanidin up to trimers are absorbed from the digestive tract and present in blood and urine. The present data also demonstrate that unconjugated catechin and epicatechin are bioavailable to the brain in rats. More quantitative studies are needed to investigate absorption, metabolism and excretion of oligomeric proantho-cyanidins.


These studies were supported in part by grants from the National Center for Complementary and Alternative Medicine and the National Institutes of Health Office of Dietary Supplements (5P50 AT-00477--to the Purdue University-UAB Botanicals Center for Age-Related Disease, Connie Weaver, PI), UAB Alzheimer Disease Research Center (1P50AG16582-08) and UAB Memory Disorder Research center. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the National Center for Complementary and Alternative Medicine, the Office of Dietary Supplements, or the National Institutes of Health.

The mass spectrometer was purchased by funds from a NIH/NCRR Shared Instrumentation Grant (S10 RR19231) and from this institution. Operation of the UAB Comprehensive Cancer Center Mass Spectrometry Shared Facility has been supported in part by a NCI Core Research Support Grant to the UAB Comprehensive Cancer (P30 CA13148). We also wish to thank Kikkoman, Inc., for providing the grape seed extract for these studies.


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Jeevan K. Prasain (a), (c), *, Ning Peng (b), Yanying Dai (b), Ray Moore (d), Alireza Arabshahi (a), Landon Wilson (d), Stephen Barnes (a), (c), (d), J. Michael Wyss (b), (c), Helen Kim (a), (c), Ray L. Watts (e)

(a) Departments of Pharmacology & Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294, USA

(b) Department of Cell Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA

(c) Purdue-UAB Botanicals Center for Age-Related Disease, University of Alabama at Birmingham, Birmingham, AL 35294, USA

(d) Comprehensive Cancer Center Proteomics/Mass Spectrometry Shared Facility, University of Alabama at Birmingham, Birmingham, AL 35294, USA

(e) Department of Neurology, University of Alabama at Birmingham, Birmingham, AL 35294, USA

* Corresponding author at: Department of Pharmacology & Toxicology, MCLM 456, University of Alabama at Birmingham, 1918 University Boulevard, Birmingham, AL 35294, USA.

Tel.: + 1205 996 2612; fax: + 1205 934 6544.

E-mail address: (J.K. Prasain).
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Author:Prasain, Jeevan K.; Peng, Ning; Dai, Yanying; Moore, Ray; Arabshahi, Alireza; Wilson, Landon; Barnes
Publication:Phytomedicine: International Journal of Phytotherapy & Phytopharmacology
Article Type:Report
Date:Mar 1, 2009
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