Ed. by Eric J. Murphy and Thad A. Rosenberger.
Methods in signal transduction series
Biochemists, molecular biologists, and other biomedical researchers describe an array of commonly used techniques for assessing lipid-mediated signal transduction. The overall themes are enzyme systems involved, mass spectrometry for lipid analysis, the lipid-mediated regulation of gene expression, and methods for assessing fatty acid and phospholipid metabolism. Specific topics include methods for measuring endocannabinoid production and expression and activity of enzymes involved in the endocannabinoid system, a high-performance liquid chromatography/tandem mass spectrometry approach to analyzing bioactive sphingolipids, fluorescence methods to assess the impact of lipid binding proteins on the ligand-mediated activity of gene expression, and a steady-state kinetic radiotracer approach to analyzing fatty acid incorporation and turnover in vivo. The anthology represents a considerable update and expansion of the 1999 Lipid Second Messengers.
([c]2010 Book News, Inc., Portland, OR)
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|Publication:||SciTech Book News|
|Article Type:||Book review|
|Date:||Jun 1, 2010|
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