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Leukoencephalomalacia in horses associated with immature corn consumption/Surto de leucoencefalomalacia em equinos associado ao consumo de milho verde.

Horse leukoencephalomalacia (ELEM) is a disease caused by the ingestion of mycotoxins (fumonisins) produced by fungi of the genus Fusarium, including F. proliferatum and F. verticillioides (Fusarium moliniforme). The disease has been described in equine fed corn grains, its byproducts (corn roll, broken corn and ground corn), corn-based pelleted or non-pelleted feed, and bran (WILSON et al., 1990, MEIRELES et al., 1994). ELEM was also described in horses fed hay, oats and mixtures of cereal screenings (WILSON et al., 1990, VENDRUSCOLO et al., 2016). Temperatures below 20[degrees]C and environmental humidity of 15% or higher predisposes to fumonisin production by Fusarium spp., associating the disease with cold seasons (RIET & MENDEZ 2007; ALBERTS et al., 1990). In the southernmost region of Brazil, ELEM occurs in the months of June to September (MEIRELES et al., 1994), but there are records of ELEM between March and December in the northern area of the country (CAMARA et al., 2008, GIANNITTI et al., 2011).

Fungal proliferation, including Fusarium spp. in corn, also depends on the grain's water activity (wa). Values ranging from zero to 0.20 indicate that water is strongly bound, while values ranging from 0.70 to 1.00 indicate that the majority of water molecules are unattached and can be used in chemical and enzymatic reactions, in addition to the development of microorganisms (CUNHA, 2018). Grain corn in the milky or pasty phase has a water activity of approximately 0.98, which can influence and enhance fungal growth.

Horses are especially sensitive to fumonisin poisoning and there are no safe levels for this species. Cases may occur with concentrations less than 1ppm, but most poisonings occur with greater than 10ppm (ROSS et al., 1991). Clinical signs are drowsiness, depression, ataxia, tremors, compulsive walking and head pressing against objects and death occurs within 24-48 hours. Lesions are localized in the white matter of the cerebral cortex and brain stem and are characterized by liquefactive necrosis, edema and hemorrhage. Histologically, there is necrosis of the white matter, edema, multifocal hemorrhages and inflammatory infiltrates of neutrophils, eosinophils and foamy macrophages. Vascular alterations can also occur, such as vascular endothelial edema, perivascular edema, hemorrhage and occasionally perivascular cuffing (MEIRELES et al., 1994, GIANNITTI et al., 2011). Lesions may not be present in discrete cases.

The aim of the present paper was to describe the epidemiological characteristics of an ELEM outbreak associated with the consumption of immature corn.

Two out of three horses belonging to a farm located at the municipality of Cangucu, RS, Brazil displayed neurological signs and death within 24 hours. On visiting the farm, a necropsy of one of the horses was performed. Epidemiological data and clinical signs were investigated. During the necropsy, samples of all organs and the central nervous system (CNS) were obtained, fixed in 10% buffered formalin, routinely processed, and stained with hematoxylin and eosin (HE). Corn samples containing all parts were collected and sent to the Laboratorio de Pesquisas Micologicas/Universidade Federal de Santa Maria for the determination of the presence and quantification of fumonisins.

According to the owner, horses exhibited hind limb rigidity, unawareness of their surroundings, proprioceptive deficits, loss of balance, aimless circling, recumbency with paddling and death within 24 hours after the onset of clinical signs. The animals were eating corn that was still immature in the beginning reproductive stage, which was harvested directly from the field with tassels, stalks, leaves, tillers, cob and kernels (Figure 1A). This corn was administered to the horses within one day after harvest. Horses ingested the corn approximately seven days before the onset of clinical signs. After the corn withdrawal, there were no more cases in the farm.

Grossly, brain exhibited dark red to brown multifocal rounded areas 2-3cm in diameter on the cut surface of the white matter of the telencephalon (Figure 1B). Additionally, areas of hippocampus e thalamus exhibited yellowish extents around the lesions (edema) adjacent to the dark red to brown areas. Histologically, there was neuropil edema and hemorrhage in the white matter scattered in several areas of the telencephalon (Figure 1C) and near the hemorrhagic areas foci of malacia, reactive astrocytes and some inflammatory cells were observed (Figure 1C). Along these lesions, degeneration and swelling of the endothelial cells of the capillaries was also observed (Figure 1D). These lesions corresponded to the dark red to brown areas observed grossly.

The diagnosis of ELEM was based on the epidemiology, clinical signs, gross and histologic lesions and the presence of 2ppm of fumonisin in the immature corn administered to the horses. This mycotoxicosis is familiar to veterinarians and horse owners and has decreased in frequency in RS due to the avoidance of corn in the horse diet, especially in winter. It has been demonstrated that the disease can occur by the ingestion of corn without the presence of visible mold and in corn with less than 15% moisture (RIET-CORREA et al., 1998). In the present case, the corn was harvested still immature and administered directly to the animals without any storage. The water activity in this cereal, when the grain is still milky, is 0.98, which can predispose it to mycotoxin-producing fungal growth (HERMANNS et al., 2006). In a fumonisin contamination study, different corn development stages were positive for the toxin presence. The toxin has been reported mainly in the farinaceous grain (R4), hard farinaceous (R5) and physiological maturation (R6) stages (HERMANNS et al., 2006). In the present case; however, fumonisin was reported in grain at the beginning of its reproductive phase (R2), suggesting that even immature corn can be contaminated with Fusarium spp. Fumonisin toxicity is cumulative and safe levels for horses have not yet been determined. Several studies have attempted to relate the mycotoxin concentration with ELEM, but outbreaks have been described with only 1 ppm in the diet that was offered to the horses (ROSS et al., 1991). Outbreaks reported in the literature have been associated with mature corn and its byproducts; however, corn in the milky grain phase has not yet been described as a cause of ELEM. The lesions observed grossly were discrete, but histologically, the lesions were characteristic of ELEM (RIET-CORREA et al. 1998). This finding suggested that the severity of the lesions is directly linked with the fumonisin concentration. Leukoencephalomalacia should be differentiated from other neurological disorders in horses, such as rabies, horse viral encephalomyelitis, horse herpesvirus type 1 infection, neoplasms and Trema micrantha poisoning. It should be taken into consideration that those diseases have acute onsets similar to that of ELEM. Similar gross lesions can be observed in the neurological form of horse trypanosomiasis (RODRIGUES et al. 2005); therefore, it also should be considered as a differential diagnosis. The history of corn feeding is a key epidemiological factor for the presumptive diagnosis; although, other foods, such as hay, oats, sweet feed and cereal mixtures from surplus crop, can also cause the disease (WILSON et al., 1990, VENDRUSCOLO et al., 2016). This case highlighted that immature corn, without evidence of mold, is an unusual source of fumonisins and can be associate with ELEM. Moreover, in the present report, the fumonisin concentration was linked with the severity of gross and microscopic lesions.

http://dx.doi.org/ 10.1590/0103-8478cr20180925

Received 11.12.18

Approved 02.01.19

Returned by the author 03.01.19

CR-2018-0925.R2

ACKNOWLEDGEMENTS

The authors are grateful to Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq: 305283/20154, 431659/2016-8), Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul and Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) (finance code 001) for scientific, financial support and student's scholarships.

DECLARATION OF CONFLICTING INTERESTS

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS' CONTRIBUTIONS

JVZE, PES, DIBP and ALS contributed equally for the conception and writing of the manuscript. LSM and LSR prepared literature revision an carried out the lab histological preparations and lesions description.

REFERENCES

ALBERTS, J.F. et al. Effects of temperature and incubation period on production of fumonisin B1 by Fusarium moniliforme. Applicated Environmental Microbiology v.56, p. 1729, 1990. Available from: <https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC184501/pdf/aem00087-0233.pdf >. Accessed: Oct. 02, 2018.

CAMARA, A.C. et al. Leukoencephalomalacia in equines in the State of Pernambuco Ciencia Animal Brasileira. v. 9, p.470-79, 2008. <https://www.revistas.ufg.br/vet/article/view/1222>. Accessed: Oct. 02, 2018.

CUNHA H.V.F A diferenca entre Atividade de Agua (Aw) e o Teor de Umidade nos alimentos. 2018. Available from: <https:// foodsafetybrazil.org/diferenca-entre-atividade-de-agua-aw-e-oteor-de-umidade-nos-alimentos/>. Accessed: Aug. 13, 2018.

GIANNITTI, F. et al. Horse leukoencephalomalacia (ELEM) due to fumonisins B1 and B2 in Argentina. Pesquisa Veterinaria Brasileira.v.31 n.5, p.407-412, 2011. Available from: <http:// www.scielo.br/pdf/pvb/v31n5/v31n5a07.pdf> Accessed: Aug. 13, 2018. doi: http://dx.doi.org/10.1590/S0100-736X2011000500007.

HERMANNS, G. et al. Fungi and fumonisins in the pre-harvest period of maize. Ciencia e Tecnologia de Alimentos. v. 26, n. 1, p. 7-10, 2006. Available from: <http://www.scielo.br/pdf/cta/ v26n1/28841.pdf>. Accessed: Aug. 16, 2018. doi: http://dx.doi. org/10.1590/S0101-20612006000100002.

MEIRELES M.C. et al. Mycoflora of the toxic feeds associated with horse leukoencephalomalacia (ELEM) outbreaks in Brazil. Mycopathologia. v.127, n.3, p.183-188, 1994. Available from: <https://www.ncbi.nlm.nih.gov/pubmed/7808512>. Accessed: Aug. 16, 2018.

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Joanna Vargas Zillig Echenique (1) Pablo Estima-Silva (1) Daniela Brayer Pereira (1,2) (iD) Lucas Santos Marques (3) Luiza Soares Ribeiro (3) Ana Lucia Schild (4) * (iD)

(1) Programa de Pos-Graduacao em Veterinaria, Faculdade de Veterinaria, Universidade Federal de Pelotas (UFPel), Capao do Leao, RS, Brasil.

(2) Instituto de Biologia, Departamento de Parasitologia e Microbiologia, Universidade Federal de Pelotas (UFPel), Capao do Leao, RS, Brasil.

(3) Laboratorio Regional de Diagnostico (LRD), Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brasil.

(4) Laboratorio Regional de Diagnostico, Universidade Federal de Pelotas (UFPel), 96010-900, Campus Universitario Capao do Leao, Capao do Leao, Pelotas, RS, Brasil. E-mail: alschild@terra.com.br. * Corresponding author.

Caption: Figure 1--A. Immature com harvested that fed the horses. Note the milky grain appearance. B. Brain fixed in buffered 10% formalin, frontal telencephalon, white matter. Discrete multifocal hemorrhage in the white matter surrounded by pale yellow areas. C. Brain, frontal telencephalon, white matter. Hemorrhage, edema and malacia with reactive astrocytes with eosinophilic cytoplasm and some inflammatory cells in white matter. HE. 200x. D. Brain, frontal telencephalon, blood vessel lined by reactive endothelium that are swollen, surrounded by eosinophilic proteinaceous fluid (edema) expanding Virchow-Robin space. HE, 400x.
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Title Annotation:PATHOLOGY
Author:Echenique, Joanna Vargas Zillig; Estima-Silva, Pablo; Pereira, Daniela Brayer; Marques, Lucas Santos
Publication:Ciencia Rural
Date:Mar 1, 2019
Words:1939
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