Printer Friendly

Lasioderma serricorne (Coleoptera: Anobiidae): first record in dehydrated bee pollen in Sergipe state, Brazil.

Coleopterans have global importance in grain storage and industrial food production and can cause damage to the appearance of products as well as weight loss and reduced nutrient levels, rendering products unfit for human consumption (Nansen et al. 2004). Lasioderma serricorne (Fabricius, 1792) (Coleoptera: Anobiidae), variously known as the cigarette beetle or the tobacco beetle, is cosmopolitan and occurs frequently in tropical and subtropical areas. This insect is usually found in perishable food products such as dry fruit, grain, crumbles, cereal flour and animal food (Papadopoulou & Buchelos 2002).

Pollen, which adheres to honey bees (Apis mellifera L.) as they collect nectar from flowers and which they agglutinate into balls with nectar and bee saliva is important for survival of these insects. Beekeepers remove pollen for processing, commercialization and human consumption as a protein source (Mattila et al. 2007; Bastos et al. 2000).

Damage to bee pollen stored at 10% RH was found in samples collected in Sergipe State, Brazil. The purpose of this study was to characterize the damaged pollen and to ascertain its cause.

Pollen samples were obtained from the producers of the municipalities of Pacatuba, Brejo Grande, Neopolis, Estancia and Tobias Barreto in Sergipe State, Brazil in Feb (summer) and Jun (winter) 2007. A bee returning to the hive after collecting nectar from flowers passes through a retention grill at the entrance that allows her passage but removes the pollen ball, which then falls into a box. This material is collected by the beekeeper, stored at 4 [degrees]C for 48 h and is air-dried at 40 [degrees]C for 48 h. Any visible impurities such as insect fragments, fibers, propolis, balls, etc., are removed from the dry material.

The pollen samples were supplied by producers in commercial packaging of plastic or polyethylene terephthalate (PET) bags. The pollen was stored in 50 mL plastic packages at an average temperature of 28 [degrees]C and 80% RH in the Agricultural and Forest Pests Laboratory of the Department of Forest Engineering of the Federal University of Sergipe.

The beetles were removed from contaminated samples and stored in 70% (v/v) alcohol. Adults were pinned in the laboratory and sent to the Taxonomy Laboratory of the Entomology and Acarology Department of ESALQ, University of Sao Paulo, for identification.

Palynological analysis was done in the Agricultural and Forest Pests Laboratory of the Federal University of Sergipe. This analysis compared pollen grains with those of an image bank to identify the botanic plant families that bees have visited to collect nectar, i.e., the images of the pollen grains were compared to those of known plant families. Microscope slides of the pollen samples were made with the acetolysis method (Erdtman 1960; Barth & Luz 1998; Terrab et al. 2003) and photomicrographed by a digital system of photo-documentation connected to an optical microscope with objectives of 40' and 100', and a quantitative assessment was made with 300 pollen grains per slide. The occurrence of each type of pollen (botanical family to which it belonged) was classified as: dominant ([greater than or equal to] 45%), accessorial (16-45%), or occasional ([less than or equal to] 15%). The water content of 2 g samples of pollen was measured gravimetrically with an infrared drying system (Mettler LP16).

Insects found in pollen samples were identified as the tobacco beetle Lasioderma serricorne (Fabricius, 1792) (Coleoptera: Anobiidae) (Fig. 1). The hard plastic packages containing the pollen samples were undamaged (Fig. 2), so their infestation must have occurred in the field or during processing and sample handling. However, this beetle can cut into plastic bags and cause problems for the food industry (Arbogast et al. 2005; Begum et al. 2007). This beetle reproduces and the larvae grow and develop into adults when feeding on stored bee pollen.

Damage to pollen balls was similar to that observed in grains, with the consumption of the internal part, leaving only a thin husk. Lasioderma serricorne larvae also feed on pollen with galleries inside the packages of these products in which they leave excretions and feces. As a result, the pollen grains become lumpy and unfit for human or animal consumption (Fig. 1).

[FIGURE 1 OMITTED]

Insect contamination was observed in dehydrated bee pollen from Neopolis, Pacatuba, Brejo Grande and Estancia municipalities which had a water content > 8% (Table 1).

[FIGURE 2 OMITTED]

The dehydrated bee pollen from Tobias Barreto was the only product not infested with L. serricorne beetles. Bee pollen from this municipality had a moisture level > 8%. Pollen from Areaceae species was < 10% of the total in bee pollen from Tobias Barreto, whereas Areaceae pollen occurred more frequently in samples from the other 4 municipalities, suggesting that L. serricorne might have a preference for this type of pollen (Table 2).

Lasioderma serricorne has been found in packaged foods destined for birds, cats and dogs in the USA (Roesli et al. 2003) and in wheat flour, maize and oat in Portugal, and it is common in factories using these cereals as raw material (Carvalho et al. 2006). This beetle infested tobacco and processed foods in Greece (Papadopoulou & Buchelos 2002). Despite its wide range of damaged products, L. serricorne has not been reported previously to infest stored pollen.

The water content of the dehydrated bee pollen samples was > 8%, which might be important for L. serricorne reproduction; however, this beetle was not found in pollen collected from Tobias Barreto. Dehydrated bee pollen is supposed to have a maximum water content of 4% but it can change during storage (Almeida-Muradian et al. 2005).

Dehydrated bee pollen contains high levels of proteins, amino acids, lipids and secondary nutrients such as vitamins, which makes it a desirable food source for L. serricorne (Shinoda & Fujiasaki 2001; Patt et al. 2003; Lundgren & Wiedenmann 2004); however, its nutrient content varies among main botanic families and species of the same family (Richards & Schmidt 1996). The pollen from Tobias Barreto without L. serricorne had lower percentage from species of the botanic family Arecaceae compared to all other samples.

This is the first report of L. serricorne in dehydrated bee pollen. This beetle damages the dehydrated pollen balls, making them unfit for human or animal consumption.

Caption: Fig. 1. Lasioderma serricorne (Coleoptera: Anobiidae), the cigarette beetle, found in dehydrated pollen. a. side view of the insect; b. dorsal view; c. damage by the cigarette beetle, Lasioderma serricorne (Coleoptera: Anobiidae) in dehydrated pollen; d. appearance of the infested product.

Caption: Fig. 2. Container with dehydrated pollen with a heavy infestation by the cigarette beetle, Lasioderma sericorne (Coleoptera: Anobiidae).

ACKOWLEGMENTS

We express thanks to "Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)", "Coordenagao de Aperfeigoamento de Pessoal de Nivel Superior (CAPES)" and "Fundagao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG)" for financial support.

REFERENCES CITED

ALMEIDA-MUEADIAN, L. B., PAMPLONA, L. C., COIMBEA, S., AND BAETH, 0. M. 2005. Chemical composition and botanical evaluation of dried bee pollen pellets. J. Food Comp. Analysis 18: 105-111.

AEBOGAST, E. T., CHINI, S. E., AND MCGOVEEN, J. E. 2005. Use of contour analysis in monitoring stored-product insects. J. Econ. Entomol. 99: 601-603.

BARTH, 0. M., AND LUZ, C. F. P. 1998. Melissopalynological data obtained from a mangrove area near to Rio de Janeiro, Brazil. J. Apicult. Res. 37: 155-163.

BASTOS, E. M., OLIVEIEA, V. D. C., AND SOAEES, A. E. E. 2000. Microscopic characterization of the green propolis, produced in Minas Gerais State, Brazil. Honeybee Sci. 21: 179-180.

BEGUM, K., EEDDY, P. V., LEELAJA, B. C., EAJASHEKAE, Y., AND EAJENDEAN, S. 2007. Studies on insect infestation in chocolates. J. Stored Prod. Res. 43: 118-122.

CARVALHO, M. 0., PASSOS DE CAEVALHO, J., TORRES, L. M., AND MEXIA, A. 2006. Developing sequential plans for classifying Lasioderma serricorne (F) (Coleoptera, Anobiidae) status in a cigarette factory. J. Stored Prod. Res. 42: 42-50.

ERDTMAN, G. 1960. The acetolysis method, a revised description. Svensk Bot Tidskr 54: 561-564.

LUNDGEEN, J. G., AND WIEDENMANN, E. N. 2004. Nutritional suitability of corn pollen for the predator Coleomegilla maculata (Coleoptera: Coccinellidae). J. Insect Physiol. 50: 567-575.

MATTILA, H. E., AND OTIS, G. W. 2007. Dwindling pollen resources trigger the transition to broodless populations of long-lived honeybees each autumn. Ecol. Entomol. 32: 496-505.

NANSEN, C., SUBEAMANYAM, B., AND EOESLI, E. 2004. Characterizing spatial distribution of trap captures of beetles in retail pet stores using SADIE[R] software. J. Stored Prod. Res. 40: 471-483.

PAPADOPOULOU, S. C., AND BUCHELOS, C. T. 2002. Identification of female adult Lasioderma serricorne (F.) by simple external observation of the abdomen. J. Stored Prod. Res. 38: 315-318.

PATT, J. M., WAINEIGHT S. C., HAMILTON, G. C., WHITTINGHILL, D., BOSLEY, K., DIETEICK, J., AND LASHOMB, J. H. 2003. Assimilation of carbon and nitrogen from pollen and nectar by a predaceous larva and its effects on growth and development. Ecol. Entomol. 28: 717-728.

RICHARDS, P. C., AND SCHMIDT, J. M. 1996. The effect of selected dietary supplements on survival and reproduction of Orius insidiosus (Say) (Hemiptera: An thocoridae). Can. Entomol. 128: 171-176.

ROESLI, E. SUBEAMANYAM, J. F. C., AND KEMP, K. 2003. Stored-product insects associated with a retail pet stored chain in Kansas. J. Econ. Entomol. 96: 19581966.

SHINODA, K., AND FUJISAKI, K. 2001. Effect of adult feeding on longevity and fecundity of the cigarette beetle, Lasioderma serricorne F. (Coleoptera: Anobiidae). Appl. Entomol. Zool. 36: 219-223.

TERRAB, A., DIEZ, M. J., AND HEEEDIA, F. J. 2003. Palynological, physicochemical and colour characterisation of Moroccan honeys: I. River red gum (Eucalyptus camaldulensis Dehnh) honey. Int. J. Food Sci. Tech. 38: 379-386.

JULIO CESAR M. PODEROSO (1), MARIA E. CORREIA-OLIVEIRA (2), JACI MENDES VIEIRA (2), GENESIO T. RIBEIRO (3) RAFAEL C. RIBEIRO (1) AND JOSE C. ZANUNCIO (1), *

(1) Departamento de Entomologia, Universidade Federal de Vigosa. Vigosa, Minas Gerais State, Brazil

(2) Departamento de Entomologia e Acarologia, Escola Superior de Agricultura Luiz de Queiroz/Universidade de Sao Paulo, Piracicaba, Sao Paulo State, Brazil

(3) Departamento de Engenharia Florestal, Universidade Federal de Sergipe, Sao Cristovao. Sergipe State, Brazil

* Corresponding author; E-mail: zanuncio@ufv.br
TABLE 1. RESULTS OF THE PERCENTAGE OF MOISTURE
IN DEHYDRATED SAMPLES OF POLLEN DURING THE
SUMMER (FEB) AND WINTER (JUN) 2007 PRODUCED
IN VARIOUS MUNICIPALITIES OF SERGIPE, BRAZIL.

Municipalities   February/2007   June/2007

Brejo Grande         10.6           9.9
Estancia             10.5          12.5
Neopolis             10.3          10.93
Pacatuba              9.83          8.96
Tobias Barreto       11.66         11.16

TABLE 2. BOTANICAL FAMILIES FOUND IN SAMPLES OF POLLEN
DEHYDRATED IN SERGIPE. D: DOMINANT POLLEN GRAIN,
[greater than or equal to] 45% OF REPRESENTATIVENESS;
A: ACCESSORIAL, 16 -45%; O: OCCASIONAL,
[less than or equal to] 15

Cities      Botanical Family

            February                    June

Brejo       Compositae (D); Arecaceae   Mimo-Arecaceae (D);
  Grande      (A); Fabaceae, Rusaceae     Compositae(A);
              and Rubiaceae (O)           Mimosaceae, biaceae
                                          and Myrtaceae (O)
Estancia    Arecaceae, Amaranthaceae,   Mi-Arecaceae,
              Compositae (A);             Compositae (A);
              mosaceae, Fabaceae and      Mimosaceae,
              Labiatae (O)                Myrtaceae, Fabaceae
                                          and Rubiaceae (O)
Neopolis    Mimosaceae (D); Arecaceae   An-Mimosaceae,
              (A); Compositae,            Arecaceae, Compositae
              arcadiaceae and             (A); Rubiaceae (O)
              Myrtaceae (O)
Pacatuba    Arecaceae (D); Compositae   Arecaceae (D);
              (A)                         Mimosaceae (A);
                                          Compositae (O)
Tobias      Myrtaceae, Solanaceae       Compositae, Labiatae
  Barreto     (A), Arecaceae,             (A); Amaranthaceae,
              Compositae and              Fabaceae, Mimosaceae
              Mimosaceae (O)              and Arecaceae (O)
COPYRIGHT 2013 Florida Entomological Society
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2013 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Scientific Notes
Author:Poderoso, Julio Cesar M.; Correia-Oliveira, Maria E.; Viera, Jaci Mendes; Ribeiro, Genesio T.; Riber
Publication:Florida Entomologist
Article Type:Report
Geographic Code:3BRAZ
Date:Jun 1, 2013
Words:1841
Previous Article:Note on photographing embryos and hatchlings of Leiobunum sp. harvestmen (Arachnida: Opiliones: Sclerosomatidae: Leiobuninae).
Next Article:June 2013 Florida Entomological Society corporate and sustaining members.
Topics:

Terms of use | Copyright © 2017 Farlex, Inc. | Feedback | For webmasters