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Laser, electrospray combo samples live cells.

Mass spectrometry technologies that effectively analyze biological samples in water exist, but often the analysis must be performed under vacuum, or--in the case of spatial characterization--requires sample preparation or the addition of a matrix.

The LAESI DP-1000 Direct Ionization System, from Protea Biosciences, Morgantown, W.V., combines laser resolution capabilities with the benefits of electrospray ionization to allow researchers to profile the distribution of biomolecules in water without the need for vacuum. This direct sample analysis of living cells results in better results and increased sensitivity without the artifacts commonly seen with sample preparation.

The LAESI DP-1000 directly ionizes samples in a two-step process: laser ablation followed by electrospray ionization. The process begins when the sample is ablated by a short, mid-infrared laser pulse that is tuned to the strong absorption line of liquid water (2,940 nm). This laser pulse creates a small plume of non-ionized material. As the plume collapses, a secondary jet of material is ejected from the sample surface and into the ESI stream where it is ionized. The ionized material is then swept into the mass spectrometer for analysis. The system can also directly profile tissue samples in three dimensions, using the same system.

Protea Bioscience Group Inc.,

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Title Annotation:Analytical Instruments; LAESI DP-1000 Direct Ionization System from Protea Biosciences
Comment:Laser, electrospray combo samples live cells.(Analytical Instruments)(LAESI DP-1000 Direct Ionization System from Protea Biosciences)
Publication:R & D
Article Type:Brief article
Geographic Code:1USA
Date:Sep 1, 2012
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