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Lab M's VCC integral to E.coli 0157 isolation.

The current UK outbreaks of Escherichia coli 0157 infection in young children have once again highlighted the seriousness of the ensuing illness, the importance of timely diagnosis and the need to track the source of infection. Enrichment of samples is an important part of the E. coli 0157 recovery and isolation process and Lab M's Buffered Peptone Water is used with VCC Selective Supplement to prepare a pre-enrichment broth for organism recovery. It then also serves as a selective enrichment medium for the isolation of E. coli 0157 from food and other samples.

As well as this enrichment broth, Lab M provides a full range of media for E. coli 0157 culture, including modified Tryptone Soy Broth (mTSB), Sorbitol MacConkey Agar (SMAC) and Cefixime Tellurite MacConkey Agar (CT-SMAC), selective differential media for the isolation of E. coli 0157:H7, the primary serovar associated with haemorrhagic colitis and haemolytic uraemic syndrome. Harlequin[TM] SMAC-BCIG is a modification of SMAC agar in which the addition of a specific chromogenic substrate (5-bromo-4-chloro-3-indoxyl-E-D-glucuronide [BCIG]) allows the straightforward differentiation of E. coli O157:H7 from other strains on the basis of colony colour.

Alongside these media is Rhamnose MacConkey (VTEC 026) Agar, selective for verocytotoxin producing E.coli 026, a strain that is also associated with haemorrhagic colitis and haemolytic uraemic syndrome. This medium is based on MacConkey agar, but substitutes rhamnose for the usual lactose as the fermentable carbohydrate. VTEC O26 colonies are unable to ferment the rhamnose present in the medium so remain translucent. All other non-O26 VTEC colonies appear as pink to red.

Contact Lab M on tel 0161 797 5729 or visit
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Comment:Lab M's VCC integral to E.coli 0157 isolation.(PLANT AND EQUIPMENT)
Publication:Food Trade Review
Date:Oct 1, 2009
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