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Inoculation methods and aggressiveness of five Fusarium species against peach palm/Metodos de inoculacao e agressividade de cinco especies de Fusarium a pupunheira.

INTRODUCTION

Peach palm (Bactris gasipaes var. gasipaes Henderson) plantations for heart of palm production have expanded in recent years in several Brazilian states including Bahia, Goias, Sao Paulo, Mato Grosso, and Santa Catarina. Its importance is mainly attributed to the substitution of the "Jucara" heart-of-palm (Euterpe edulis Martius), exploited in an extractive manner in the Atlantic Forest, by a cultivated palm allowing successive cuts for several years. Peach palm exhibits differentiated characteristics, such as tillering, precocity, and no oxidation of the heart of palm after cutting, enabling its in natura commercialization (SANTOS et al., 2008).

Fusariosis, caused by Fusarium spp., is a major disease which affects the peach palm. Fusariosis causes leaf yellowing, which progresses until the plant death, and occasionally, of clump, with reduction of stands in nurseries and adult plantations (PIZZINATTO et al., 2001). Fusarium can be disseminated by seeds, and it is present in the main production areas in Brazil (SANTOS et al., 2008; COSTA JUNIOR et al., 2016). According to COSTA JUNIOR et al. (2016), fusariosis may be limiting factor for the expansion of peach palm plantations.

Several Fusarium species (F. proliferatum, F. verticillioides, F. anthophilum, F. oxysporum, F. solani, and F. subglutinans) are linked to peach palm fusariosis (PIZZINATTO et al., 2001; ALMEIDA et al., 2005). However, information on the aggressiveness of these species against the peach palm and appropriate methods of pathogen inoculation is lacking.

One difficulty in studying fusariosis is related to the inoculation methods (COSTA et al., 2012; KLINGELFUSS et al., 2007). Studies have experienced difficulty in reproducing the same results (PIZZINATO et al., 2001; COSTA JUNIOR, 2013). The symptoms observed in the field are not reproduced in inoculation tests, taking up to eight weeks for typical symptoms appearance (ALVES et al., 2006, PIZZINATTO et al., 2001). Furthermore, extensive injury to inoculated plants has been reported, resulting in plant death due to injuries caused during inoculation (COSTA JUNIOR, 2013). Additionally, some studies produced non-reproducible results in successive experiments using the same strain (PIZZINATO et al., 2001; COSTA JUNIOR, 2013). Thus, the objective of this research was to evaluate inoculation methods and aggressiveness of isolates of five Fusarium species against peach palm.

MATERIALS AND METHODS

Origin of isolates of Fusarium spp.

Thirty-one isolates of Fusarium spp. were collected from five Brazilian states (Table 1) from the Fungi collection of the Forest Pathology Laboratory (Embrapa Florestas, Colombo-PR). They included F proliferatum, F. verticillioides, F. oxysporum species complex (FOSC), Fusarium solani species complex (FSSC), and Gibberella fujikuroi species complex (GFSC). The Fusarium spp. isolates were obtained from peach palm plants with fusariosis symptoms from plantations located in the states of Sao Paulo, Rondonia, Parana, and Santa Catarina.

Preparation of inoculums

The Fusarium spp. isolates were grown on potato dextrose agar (PDA) medium for 15 days in the dark at 24[degrees]C. Conidial suspensions were each added to 10mL of sterile distilled water in a Petri dish and calibrated using a Neubauer chamber to reach 1 x [10.sup.6] conidia x [mL.sup.-1]. To prepare ground corn (broken corn passed through a 1.6-mm sieve) for culturing Fusarium spp. isolates, the material was autoclaved for 30 minutes at 121[degrees]C and one atmosphere. Subsequently, five discs (4mm in diameter) of PDA medium with Fusarium mycelia were added to flasks containing ground corn (200g of ground corn and 40mL of water) as described by COSTA et al. (2012). Flasks were incubated for 15 days.

Experiment 1. Inoculation of F. proliferatum onto leaves, stems, and roots

The abaxial sides of leaves of fourmonth-old seedlings with an average height of 10cm were inoculated with a conidial suspension of F. proliferatum [SP-3 isolate, analyzed in a previous study (COSTA JUNIOR, 2013)] using a fine needle syringe with a diameter of 0.4mm. Ten replicates were used, with each replicate being one plant. Two treatments were used: pathogen and control. Conidial suspension was infiltered through the mesophyll leaf until it was possible to visualize a wet region. In the control treatment, the same procedure was performed using only infiltered saline solution (0.85% NaCl) on the leaves.

Stems of 10 peach palm seedlings were inoculated using the wounding method in the basal region, and a micro-drop of 5[mu]L of the suspension (1 x [10.sup.6] conidiax [mL.sup.-1]) was deposited on the wounded region. This wound was created using a needle 1.6mm in diameter. F. Proliferatum isolate SP-3 was used. For the third inoculation, roots were washed and 20[mu]L of the conidial suspension (1 x [10.sup.6] conidia x [mL.sup.-1]) was injected into three young roots using a syringe equipped with a fine needle. The control group received only one injection with water. Evaluations were conducted weekly using a diagrammatic scale (COSTA JUNIOR, 2013) to determine the severity of the disease.

Experiment 2. Evaluation of aggressiveness of 31 isolates of five Fusarium species

Injured stems were infected with the conidial suspension, and the conduction of 10 plants per isolate was analyzed in tubes (adapted from DIHAZI et al. (2012)). Assessments were performed weekly to determine the incidence and severity of the disease using a diagrammatic scale (COSTA JUNIOR, 2013). When plants reached grade four, the pathogen was reisolated. The injured tissue fragments were disinfected with 70% ethanol for 30s and 1% sodium hypochlorite for 1min, and added to PDA medium. At the end of the experiment, a longitudinal cut was made in the stem and internal lesions were evaluated. Plants were distributed in a completely randomized design with 10 replicates. Each plant was an experimental unit, and the Scott-Knott test at 5% significance was conducted to determine the means of the five largest internal lesions.

Experiment 3. Comparison of methods of Fusarium inoculation onto roots

In this experiment, four methods of Fusarium sp. inoculation were evaluated. The first method was root inoculation with conidial suspension. Roots were inoculated via injection as described above. However, plants were maintained in sterile commercial substrate. Plants of the control group were injected only with sterile water. The second method was root immersion in conidial suspension. In this treatment, approximately 150mL of the conidial suspension (1 x [10.sup.6] conidia x [mL.sup.-1]) was used, in which the previously cut roots were immersed for 5 minutes. Immediately after this, the seedlings were planted in pots containing sterile substrate. The control was immersed in sterile water for the same period. The third method was root wounding and inoculation of conidia without seedling transplant. In this procedure, plants were maintained in containers and inoculation involved root wounding and subsequent addition of 10mL of conidial suspension (1 x [10.sup.6] conidia [mL.sup.-1]) to this region. In the control treatment, 10 plants received only sterile water. The fourth method was inoculation with colonized ground corn. Ground corn fusarium-colonized was added to the substrate in the proportion of 40g x [dm.sup.-3]. After mixing the inoculum to the substrate, seedlings two months of age were planted, previously cut in the apical region of roots, with previously flamed scissors. The control plants had the roots cut and were planted in pots containing 40 g.dm-3 of ground corn without fungus. The design was completely randomized with 10 replicates.

Plants were kept in a greenhouse, and after 120 days plants were oven dried at 60[degrees]C and the total dry weight, dry weight of the roots, and dry weight of leaves and stem were determined. Number of leaves, plants height, and the severity of the disease were also evaluated. Severity was calculated by dividing the total sum of the lesions by roots dry weight, in order to homogenize the results, since plants with a small amount of roots have relatively less area to be colonized by the fungus.

RESULTS AND DISCUSSION

This study demonstrated that F proliferatum can infect the leaves, stem, and roots of the peach palm. F. proliferatum, FOSC, F verticillioides, FSSC, and GFSC were verified to be pathogenic to the peach palm. The use of Fusarium-colonized ground corn for root inoculation was effective and reduced damage to plants.

At 7 days after inoculation (DAI), leaves inoculated with F. Proliferatum exhibited symptoms, characterized by blackening of leaf blade and veins (Figure 1A-C). In the re-isolation, the presence of F proliferatum in all the leaf fragments was verified. Plants inoculated with saline alone showed no symptoms. At 15 DAI, the plants whose stems were inoculated with F. proliferatum showed characteristic external symptoms, including flag leaf yellowing, similar to that of fusariosis observed in the field. At 90 DAI, cut stems displayed tissues that were darkened internally (Figure 1G-I). Certain plants of the control group showed flag leaf yellowing. On cutting the stem, the tissues of the inoculated region were darkened internally (Figure 1H), and no symptoms appeared at the injured region. Plants inoculated with F. proliferatum in the roots exhibited lesions reaching 15.2mm in length (Figure 1F). There were no reflex symptoms in the upper parts of the inoculated plants. Isolates SP-3 and SP-5 resulted in the development of lesions up to 15.21mm and 12.3mm in length, respectively. In the re-isolation, 80% recovery of F proliferatum was verified in the inoculated plants. Plants of the control group showed only dimming at the region of the inoculation wound.

Leaves, stems, and roots of peach palm were susceptible to F. proliferatum. Although, Fusarium is a soil pathogen (LESLIE & SUMMERELL, 2006), its occurrence as a leaf pathogen has been reported for other palms, such as the date palm (HODEL, 2009). However, this is the first observation of susceptibility of peach palm leaves. Owing to its practicality and speed in obtaining results, the foliar inoculation method proved to be feasible for demonstrating the pathogenicity of Fusarium. However, it has the drawback of not reproducing the typical fusariosis symptoms.

Inoculation of the wounded stems reproduced the typical symptoms of fusariosis, which were characterized by flag leaf yellowing, followed by the yellowing of the other leaves (Table 1). However, wounds created during inoculation resulted in the death of up to 50% of the plants. In the longitudinal cuts on the stem, the inoculated regions were observed to occasionally be located near the apical meristem, resulting in irreversible damage to the plants (Figure 1H). Another aspect of this method that merits attention was that plants inoculated with Fusarium were externally asymptomatic. However, in the longitudinal cuts on these plant stems, active lesions reaching up to 95mm of length were observed. This finding is important regarding the dissemination of the pathogen by asymptomatic seedlings in nurseries. In the re-isolation, the presence of Fusarium was verified in the stem fragments of plants of the control group. The endophytic origin of Fusarium in the cauline apices of peach palm has been previously reported by ALMEIDA et al. (2005). Similarly, other authors reported the presence of phytopathogenic fungi including Deightoniella torulosa and Colletotrichum spp. as endophytes in other hosts (PHOTITA et al., 2004; VIEIRA et al., 2014).

Plants inoculated with Fusarium exhibited typical symptoms of the disease, characterized by leaf yellowing that progressed until plant death. In this method (Experiment 2), disease incidence ranged from 10-80% upon inoculation with the most aggressive Fusarium isolates BG-14 and CA-1. In this study, in the control treatment, certain plants showed flag leaf yellowing, which caused the death of 50% of the plants. This phenomenon has already been observed in other studies, while inoculating peach palm stems, with injury (COSTA JUNIOR, 2013). This situation is more common while using methods causing injury, and death results from damage to the meristematic region of the plant (Figure 1G-I) as discussed previously.

In the longitudinal sections of the stems of the seedlings inoculated with Fusarium, asymptomatic plants displayed characteristic internal symptoms that included tissue darkening (Figure 1-I). Moreover, evaluation of F. proliferatum in Phoenix dactylifera L. was conducted three months after inoculation, demonstrating that diseases caused byFusarium. in palm trees may slowly progress to demonstrate external symptoms (ABDALLA et al., 2000). The CA-1A isolate of Fusarium that was initially one of the most aggressive did not maintain the same behavior in the second experiment. The same was observed for the SAF-7 isolate, while the BG-14 isolate maintained the same behavior in both experiments. This non-reproducible behavior for some Fusarium isolates following inoculation for stem wounds has already been reported by other authors in peach palm (PIZZINATO et al., 2001; COSTA JUNIOR, 2013).

In the comparison of the four inoculation methods (Figure 2), principal component analysis (PCA) revealed the influence of the treatments on the response variables, in which the severity vectors were positioned in the opposite direction to the vectors that indicated plant growth (height, dry weight of roots, and total dry weight). A location closer to the origin indicated a reduced contribution of the variable to the analysis, and the vectors of relative severity to control (S-Test) were always closer to the origin than the vectors of severity to the isolates (S-SP-3, S-SP-5), except for the injection method (Figure 2). These results indicated that this method causes greater injury in the control compared to the symptoms of the inoculated plants.

In all treatments, the principal components 1 and 2 explained most of the total variance with proportions of 67% up to 79%, which were similar to those obtained by KUHNEM JUNIOR et al. (2013), and which explained 74.5% of the total variance between isolated from Fusarium spp. in corn. In this study, F. Proliferatum isolate SP-3 inoculated using the Fusarium-colonized ground corn method resulted in a lower plant height of 72mm, compared to the control height of 76mm. The other variables analyzed, total dry weight and dry weight of roots, also gave the same indication that inoculation with Fusarium-colonized ground corn reduced plant growth without affecting the control, since the self-value of severity in this treatment for the control was very low (0.05 in the main component 1). The root immersion treatment performed satisfactorily; however, the control group values were much higher (0.27). The reduction of growth after inoculation with F. verticillioides via soil is known for the maize pathosystem, mainly at high levels of the inoculum (GLENN et al., 2008; COSTA et al., 2012).

The root wound method was the least aggressive because it did not transplant the seedlings during the inoculation. As a result, fewer roots injuries resulted in larger plants with higher dry weight. However, in this treatment less disease severity was found, which is not desirable for an inoculation method. Dry weight of roots reduced in the treatment with Fusarium-colonized ground corn, and the plants had on average 0.13g of roots for the SP-3 isolate. This likely was a result of the colonization of the roots by F. proliferatum (Figure 1D-F), which requires a substrate for its establishment and survival until the time of colonization (COSTA et al., 2012).

CONCLUSION

F. proliferatum caused lesions on leaves, stem, and roots of the peach palm. F. proliferatum, F. oxysporum species complex, F. verticillioides, F solani species complex, and Gibberella fujikuroi species complex were pathogenic to the peach palm. Root inoculation with Fusarium-colonized ground corn was the most suitable method for fusariosis.

http://dx.doi.org/10.1590/0103-8478cr20170462

ACKNOWLEDGEMENTS

We thank Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for financial support.

REFERENCES

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ALVES, S.A.R. et al. Patogenicidade de isolados de Fusarium spp. e Phytophthora palmivora associados com a Podridao do Estipe da Pupunheira no Parana. Boletim Pesquisa Florestal, v.52, p.133-140, 2006. Available from: <https://pfb.cnpf.embrapa.br/pfb/ index.php/pfb/article/view/218>. Accessed: Jun. 07, 2017.

COSTA JUNIOR, J.C. Qualidade sanitaria e fisiologica de sementes de pupunheira submetidas a tratamento quimico e caracterizacao dos sintomas da podridao da base do estipe causada por Fusarium spp. 2013. Curitiba: UFPR. 127p (Dissertacao Mestrado).

COSTA JUNIOR, J.C.; et al. Sanitary and physiological quality, methods of detection and treatment of seeds of peach palm. Ciencia Florestal, v. 26, n. 4, p.1119-1131, 2016. Available from: <http://www.redalyc.org/html/534/53449110008/>. Accessed: Jul. 05, 2017.

COSTA, R.V. et al. Metodologia para avaliacao da reacao de genotipos de milho a Fusarium verticillioides em casa de vegetacao. Circular tecnica Embrapa Milho e Sorgo, v.179, p.1-7, 2012. Available from: <https://www.researchgate.net/profile/Dagma_ Da_Silva2/publication/263785369_Metodologia_inoculacao_ fusarium_casa_de_vegetacao/links/0c96053be74e148a85000000/ Metodologia-inoculacao-fusarium-casa-de-vegetacao.pdf>. Accessed: May 26, 2017.

DIHAZI, A. et al. Use of two bacteria for biological control of bayoud disease caused by Fusarium oxysporum in date palm (Phoenix dactylifera L) seedlings. Plant Physiology Biochemistry, v.55, p.7-15, 2012. Available from: <https://www.sciencedirect. com/science/article/pii/S0981942812000605>. Accessed: Jul. 05, 2017. doi: 10.1016/j.plaphy.2012.03.003.

GLENN, A.E. et al. Transformation-mediated complementation of a FUM gene cluster deletion in Fusarium verticillioides restores both fumonisin production and pathogenicity on maize seedlings. Molecular Plant-Microbe Interactions, v.21, n.1, p.87-97, 2008. Available from: <https://apsjournals.apsnet.org/doi/abs/10.1094/ MPMI-21-1-0087>. Accessed: May 22, 2017. doi: 10.1094/ MPMI-21-1-0087.

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VIEIRA, W.A.S. et al. Endophytic species of Colletotrichum associated with mango in northeastern Brazil. Fungal diversity, v. 67, p. 181-202, 2014. Available from: <https://link.springer.com/article/10.1007/s13225014-0293- 6>. Accessed: Sept. 15, 2016. doi: 10.1007/s13225-014-0293-6.

Tiago Miguel Jarek (1) * (iO) Alvaro Figueredo dos Santos (2) Dauri Jose Tessmann (3) Elisa Serra Negra Vieira (2)

(1) Universidade Federal do Parana (UFPR), 80035-050, Curitiba, PR, Brasil. E- mail: tiagomiguel87@yahoo.com.br. * * Corresponding author.

(2) Empresa Brasileira de Pesquisa Agropecuaria (Embrapa Florestas), Colombo, PR, Brasil.

(3) Universidade Estadual de Maringa (UEM), Maringa, PR, Brasil.

Caption: Figure 1--A) Control treatment, leaf of peach palm inoculated with water. B) Symptoms on leaf inoculated with Fusarium proliferatum 7 days after inoculation (DAI). C) Symptoms on leaf 15 DAI. D) Longitudinal section root of noninoculated control treatment (7.5 x magnification). E) Control root inoculated with water (7.5x magnification). F) Symptoms in roots inoculated with F. proliferatum (7.5xmagnification). G) Non- inoculated control in cross-sectional view. H) Control stem inoculated with water. I) Stem inoculated with Fusarium proliferatum. J) Roots of control for test maintained on substrate. K) Roots of the plant treated with ground corn without fungus (control). L) Roots of plant inoculated with F. proliferatum-colonized ground corn.

Caption: Figure 2--Biplot of response height (H), total weight (PT), root weight (PR), and severity (S) for analysis of main components using correlation matrix with SP-5 and SP-3 treatments and control (Test) for inoculation methods: A) injection, B) immersion, C) injury, and D) ground corn.
Table 1--Aggressiveness against peach palm and origin of isolates of
Fusarium proliferatum, F. oxysporum species complex (FOSC), F.
verticillioides; F. solani species complex (FSSC), and Gibberella
fujikuroi species complex (GFSC) via inoculation of stem after
wounding.

Species               Strain    Origin   External symptoms

                                         INC(%)   SEV (1)

                      Control             A *      B **

F. proliferatum        SP--5      RO       30       1.2
                      Fus--13     PR       0         0
                       SP--3      RO       0         0
                      Fus--12     PR       0         0
                      Fus--2      PR       0         0
                       GA--3      SC       20       0.8
                      SAF--2      PR       10       0.4
                      CA--1 A     SP       50        2
                      SP--3HR     RO       10       0.1
                      SAF--4      PR       30       1.2
                      SAF--7      PR       0         0
                      SAF--8      PR       0         0

FOSC                    1 A       RO       20       0.6
                       1A02E      RO       30       0.6
                        A7        RO       10       0.4
                      BG--11      PR       10       0.1
                       GA--1      SC       20       0.3
                      BG--14      MG       80       2.4
                      CA--1B      SP       0         0
                       CA--2      SP       10       0.4

F. verticillioides      3 A       RO       10       0.2

FSSC                  BG--13      PR       0         0
                       SP--2      RO       20       0.6

GFSC                  BG--21      PR       20       0.8
                       BG--2      PR       0         0
                      SAF--5      PR       10       0.2
GFSC                  BG--12      PR       10       0.4
                       CA--3      SP       0         0
                      BG--15      PR       20       0.6
                      SAF--6      PR       0         0
                      SAF--3      PR       0         0

Coefficient of Variation %

Species               Strain    Internal symptoms

                                INC(%)   SEV (2) *

                      Control    A *        4.2      b (B) *

F. proliferatum        SP--5      50       17.6         a
                      Fus--13     30        6.6         b
                       SP--3     100       15.5         a
                      Fus--12     70       12.3         a
                      Fus--2      60       11.6         a
                       GA--3      60       11.4         b
                      SAF--2      30        4.1         b
                      CA--1 A     60        9.6         b
                      SP--3HR     50        6.2         b
                      SAF--4      80       11.4         a
                      SAF--7      80       13.4         a
                      SAF--8      80        7.6         b

FOSC                    1 A       50       19.6         a
                       1A02E     100       30.3         a
                        A7        40        4.3         b
                      BG--11      80       11.1         a
                       GA--1      60       19.3         a
                      BG--14      60        4.3         b
                      CA--1B      40        6.3         b
                       CA--2      90       14.2         a

F. verticillioides      3 A       50       13.2         a

FSSC                  BG--13      50       13.0         a
                       SP--2      50        4.6         b

GFSC                  BG--21      80        7.3         b
                       BG--2      50        6.5         b
                      SAF--5      70       15.3         a
GFSC                  BG--12      50        4.2         b
                       CA--3      60       13.5         a
                      BG--15      60       10.2         a
                      SAF--6      50        9.0         b
                      SAF--3     100       21.1         a

Coefficient of Variation %                 43,4

INC, Incidence (percentage of plants with symptoms); SEV (1),
Severity--Scale with grades from 0 to 4; [SEV.sup.2], Severity--Stem
lesion size (mm); * Numbers followed by the same letter within the
column do not differ statistically, Scott-Knott test, p <0.05; A *
Plants of the control group suffered injuries during inoculation,
resulting in the death of certain plants. B * Owing to injuries, the
control plants exhibited leaf
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Title Annotation:CROP PRODUCTION
Author:Jarek, Tiago Miguel; dos Santos, Alvaro Figueredo; Tessmann, Dauri Jose; Vieira, Elisa Serra Negra
Publication:Ciencia Rural
Date:Apr 1, 2018
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