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Inhibition of colon cancer with Azadirachta indica.

Roy M, Kobori M, Takenaka M, Nakahara K, Shinmoto H, Tsushida T. 2006. Inhibition of colon cancer (HT-2) cell proliferation by a triterpenoid isolated from Azadirachta indica is accompanied by cell cycle arrest and upregulation of P21. PlantaMed 72:917-23. (CB)

HT-29 human colorectal adenocarinoma cells were treated with nimbolide from Azadirachta indica (neem) for 12, 24, 36 and 48h. Nimbolide was found to cause extensive morphological changes to HT-29 cells. More than 50% of cells became detached from the dishes and displayed shrunken morphology when the cells were exposed to 5[micro]M of nimbolide for 12h. These changes were not observed in control cells.

Nimbolide also inhibited HT-29 cell growth by 3568% after 12h and 57-89% after 48h.

Cells were stained with annexin V and PI to determine the nimbolide-induced apoptotic death of HT-29 cells. Nimbolide caused an increase in apoptosis of 12.2% at treatment doses of 5[micro]M and 14.9% and doses of 10[micro]M after 48h.

This study indicates that nimbolide inhibits the growth of colon cancer cells in vitro.

Jerome Sarris

3 Widgee Place, Chapel Hill Qld 4069

Cara Barnes

20 Brendon Street, Tarragindi Qld 4121
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Publication:Australian Journal of Medical Herbalism
Article Type:Brief article
Geographic Code:8AUST
Date:Dec 22, 2006
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