Influence of the Blue Coral Heliopora coerulea on Scleractinian Coral Larval Recruitment.
It is widely recognized that space is limited in reef ecosystems. Hence, reef-associated organisms, including scleractinian corals, developed competitive mechanisms that enable them to occupy, expand, and protect their territories in pursuit of food and to ensure reproductive success . Mechanisms for competition in reef ecosystems occur in various forms, including overgrowth and direct consumption of neighboring organisms, or through allelopathy [1, 2].
Although the role of allelopathy in shaping the structure of a reef community is well-established only a few studies have examined its effect on the settlement of juvenile corals, a mechanism that could effectively exclude late entrants from conquering the occupied space. Allelopathy is likely the means by which the soft coral Xenia puertogalerae  and the scleractinian coral Porites cylindrica  established dominance and developed monospecific stands in some Philippine reefs. Because allelopathic compounds are released in the water column, hydrographic processes play a role in dispersing them. Therefore, the prevailing current direction helps shape the resulting distribution of subordinate organisms or the general pattern of the underwater landscape. For example, a study on the Great Barrier Reef revealed that allelopathic compounds emitted by Sinularia flexibilis can inhibit the recruitment of juvenile scleractinian corals located downstream of exuding colonies .
Although allelopathy is common in octocorals, larval recruitment of scleractinian corals could also be influenced by other mechanisms, such as the extrusion of mesenterial filaments  and deployment of sweeper tentacles . For example, the Caribbean octocoral Erythropodium extends long sweeper tentacles to sweep and clear all nearby substrate of any potential competitors .
The blue coral, Heliopora coerulea (order Helioporacea), is one of the most dominant reef-building corals in the Bolinao Reef Complex, northern Philippines. It constituted about 1% of coral cover on the reef in the 1990s  and increased to more than 50% a couple of decades later . The factors contributing to this increase remain to be determined. The present study thus sought to explore whether H. coerulea, like other octocorals, exhibits inhibitory effects on the larvae of other corals. A field experiment was conducted to test this hypothesis.
2. Materials and Methods
2.1. Recruitment Assay. The study was conducted within a 10 x 10 m area at a depth of 5 to 6 m on the reef crest of Malilnep channel in the Bolinao Reef Complex in northwestern Philippines (N16.44011, E119.93533). This site was chosen because of high larval entrainment demonstrated in past studies [9, 11] compared to other areas in the reef complex, hence, ensuring the availability of larval recruits during the experiment. Fiber-reinforced cement tiles  each measuring 100 x 100 x 7 mm (total surface area 228 [cm.sup.2]) were placed either inside H. coerulea aggregations ranging from half meter to two meters in diameter or in open spaces with dead coral or rocky substrate located about 2-3 meters away from H. coerulea colonies, scleractinian corals, and other reef biota (Figure 1). Four replicates consisting of four tiles per set were used for each treatment or a total of 16 tiles per treatment. The settlement tiles were fixed horizontally to the substrate by hammering two-inch concrete nails through a hole bored at the center of each tile. Settlement tiles were deployed for three months from April to June 2012, which corresponds to the reported peak spawning period of scleractinian corals, as well as H. coerulea, in Bolinao [13,14]. After the exposure period, tiles were retrieved and newly settled corals were identified to genus level by microscopic examination of all the sides of the tiles. Coral recruits were identified to the lowest taxonomic classification based on an available identification guide .
2.2. Statistical Analyses. To determine if H. coerulea significantly inhibited scleractinian coral recruitment, nonparametric statistical analysis, specifically the chi-square test, was conducted. The null hypothesis is that there is no difference in the number of recruits that settle on tiles placed adjacent to H. coerulea versus those placed at a distance away from the coral.
3. Results and Discussion
Successful recruitment is an important contributor to reef resilience in the face of environmental perturbation [16-18]. Interestingly, although recruitment tiles were deployed during the predicted period of mass spawning of scleractinian corals and H. coerulea in Bolinao [13,14], only 1 out of 16 tiles placed inside H. coerulea aggregations contained any visible recruits. The single recruit found on these settlement tiles was a blue coral juvenile (Figure 2). On the other hand, coral recruits belonging to various scleractinian genera, as well as the blue coral, were observed on 13 out of 16 tiles placed on rocky substrate away from H. coerulea aggregations. Most of the recruits were observed on the sides and bottom of the tiles with none settling on the top surface, which is consistent with reports from other studies .
Tiles placed adjacent to H. coerulea colonies showed a very low density of recruitment (0.06 [+ or -] 0.13 recruits per tile) (Table 1). In contrast, more larval recruits settled on tiles that were placed away from H. coerulea (1.44 [+ or -] 0.31 recruits per tile). The chi-square test showed a significant difference between treatment and control tiles (p = 0.0001). These findings suggest that the presence of H. coerulea can inhibit larval coral settlement. However, the exact mechanism involved is still unknown. While allelopathy is a common mechanism by which cnidarians can inhibit competition [3, 19-21], we cannot yet rule out other possible mechanisms, including the consumption of settling larvae by H. coerulea or the deployment of sweeper tentacles .
Juvenile corals belonging to Pocillopora comprised 43% of recruits identified on tiles placed away from H. coerulea (Figure 2 and Table 1), followed by Acropora (13%), Heliopora (9%), Porites (9%), and other species (26%). Interestingly, only a single H. coerulea juvenile was found on the tiles placed directly on H. coerulea colonies. This suggests that H. coerulea can inhibit the settlement of larvae of scleractinian corals, but in addition it can also exert a negative influence on conspecifics. The negative effect of corals on conspecifics has previously been documented for corals, such as Platygyra daedalea and Fungia fungites, which have been found to produce chemicals with toxic effects on their own larvae .
The composition of recruits observed in this study is consistent with observations from other coral recruitment studies that have been conducted in the Bolinao Reef Complex [11, 22]. Most corals in the area, including Acropora, Heliopora, Porites, and Favia, spawn gametes or release larvae during the months of April and May . The annual coral spawning event in Bolinao is extended, with some corals starting to spawn as early as March. However, similar to this study, the most common and abundant recruits reported in studies from the Bolinao Reef Complex belong to Pocillopora, which is known to release planulae every month . A study of coral recruitment in the Malilnep channel using the same recruitment substrate used in the present study yielded a higher number of Acropora and Pocillopora recruits compared to other species .
H. coerulea larvae have a short larval duration and their range of dispersal is determined largely by prevailing water currents on the reef . In this study, however, we did not determine the influence of water turbulence or local currents in the area where the tiles were placed. Further studies that look at recruitment in other areas on the reef and other time points need to be conducted. Further research is also needed to elucidate the exact mechanisms behind the inhibitory effect of H. coerulea on larval recruitment. We lack evidence concerning the competitive mechanisms involved, such as the possible deployment of mesenterial filaments or sweeper tentacles, because we did not conduct nighttime observations to detect these types of behaviors, which often are exhibited nocturnally by corals. Allelopathy also is a commonly used competitive mechanism in octocorals and has been observed in S. flexibilis , X. puertogalerae , Lobophytum pauciflorum, Sinularia pavida, Xenia sp. aff. danae , and Sarcophyton glaucum . It would be of interest to determine whether H. coerulea exudes allelopathic chemicals or uses other competitive mechanisms.
The inhibitory effect exhibited by H. coerulea can reinforce ecological feedback , which could lead to more persistent H. coerulea dominance. Competition with H. coerulea can adversely affect scleractinian coral growth, fecundity, and survival , resulting in further reduction of larval supply that may already be low due to the decreased number of scleractinian adults in the area. These factors bring about the Allee effect, whereby coral recruitment declines due to reduced fertilization success exacerbated by declining coral density . This indicates that a reef with a high percent cover of H. coerulea is unlikely to be a hospitable environment for the establishment of new scleractinian coral recruits. The ability of H. coerulea to inhibit larval settlement may thus have far-reaching implications on future reef community structure.
Conflicts of Interest
The authors declare that there are no conflicts of interest regarding the publication of this paper.
This study was conducted under the auspices of the Department of Science of Technology through the RESILIENTSEAS project. The authors received support from the University of the Philippines System Emerging Interdisciplinary Research Grant (OVPAA-EIDR-C04-005). R. Martinez, F. Panga, and M. Deocadez assisted in the field surveys. This is contribution 450 of the University of the Philippines Marine Science Institute.
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Michael Atrigenio, Porfirio Alino, and Cecilia Conaco
Marine Science Institute, College of Science, University of the Philippines
Diliman, Quezon City, Philippines
Correspondence should be addressed to Michael Atrigenio; email@example.com
Received 16 November 2016; Revised 10 March 2017; Accepted 2 April 2017; Published 12 April 2017
Academic Editor: Yehuda Benayahu
Caption: FIGURE 1: Four sets of four tiles each were placed either directly inside H. coerulea aggregations (a) or on rocky substrate away from the coral (b). Replicate sets of tiles were placed at least 2-3 m away from each other within a reef area of about 10 x 10 m at 5- 6 m depth in the Malilnep channel, Bolinao Reef Complex, northwestern Philippines.
Caption: FIGURE 2: Composition of recruits on settlement tiles placed either inside H. coerulea aggregations (blue bars) or in open space away from H. coerulea (red bars).
TABLE 1: Counts of larval recruits on settlement tiles placed within H. coerulea aggregations or away from H. coerulea. Four tiles were deployed per replicate (n = 4). Total surface area of each tile is 228 [cm.sup.2]. Acropora (Acr), Heliopora (Hel), Pocillopora (Poc), and Porites (Por). Larval recruits were found on the underside of the settlement tiles except for two recruits belonging to Pocillopora, which settled on the side of the tile. Recruits per tile on H. coerulea aggregation Acr Hel Poc Por Other Total Replicate 1 0.00 0.00 0.00 0.00 0.00 0.00 Replicate 2 0.00 0.25 0.00 0.00 0.00 0.25 Replicate 3 0.00 0.00 0.00 0.00 0.00 0.00 Replicate 4 0.00 0.00 0.00 0.00 0.00 0.00 Recruits per tile 0.00 0.25 0.00 0.00 0.00 0.25 % recruits 0% 100% 0% 0% 0% Mean 0.00 0.06 0.00 0.00 0.00 0.06 SD 0.00 0.13 0.00 0.00 0.00 0.13 Recruits per tile away from H. coerulea Acr Hel Poc Por Other Total Replicate 1 0.25 0.25 0.50 0.00 0.00 1.00 Replicate 2 0.00 0.00 0.75 0.25 0.50 1.50 Replicate 3 0.25 0.00 0.75 0.00 0.75 1.75 Replicate 4 0.25 0.25 0.50 0.25 0.25 1.50 Recruits per tile 0.75 0.50 2.50 0.50 1.50 5.75 % recruits 13% 9% 43% 9% 26% Mean 0.19 0.13 0.63 0.13 0.38 1.44 SD 0.13 0.14 0.14 0.14 0.32 0.31
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|Title Annotation:||Research Article|
|Author:||Atrigenio, Michael; Alino, Porfirio; Conaco, Cecilia|
|Publication:||Journal of Marine Biology|
|Date:||Jan 1, 2017|
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