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Indian craniometric variability and affinities.

1. Introduction

This paper analyses the metrical variability of human crania within the Indian subcontinent and uses the results to inform a univariate, bivariate, and multivariate comparison of Indian and other crania. India's importance for understanding anatomically modern human origins is widely recognised: India has the highest genetic diversity of any continental region after Africa [1] and is generally regarded as the major dispersal centre for Homo sapiens following our exodus from Africa [2]. Yet India has been comparatively neglected in human craniometric studies, for instance, in being excluded from the global survey of modern human crania undertaken by Howells [3]. Studies that have included Indian crania have been restricted to specimens held in overseas collections [4-10]. In addition, most of these studies have been based on a limited set of measurements, and none of them combine a presentation of descriptive statistics with a large-scale multivariate analysis of the data. The motivation of our paper is to explain Indians' craniometric affinities in the context of a thoroughgoing statistical description of Indian crania (see Supplementary Material available online at

Previously undertaken multivariate studies are consistent in pointing to a similarity between crania from India and from surrounding locations. Stock et al. found that both northern and southern Indian crania cluster tightly together. Closest to Indians are crania from Afghanistan and Iran, the Andaman Islands, Sri Lanka (Veddas and to a lesser degree Sinhalese), and at a greater remove southwest Asia [9]. Similarly, Wright found that his Indian sample clusters with Andaman Islanders, the latter being otherwise close to southwest Asians and Egyptians [10]. Brace et al. found that northern and southern Indians constitute a discrete cluster, along with Sri Lanka crania. These South Asians cluster with Europeans if Andamanese are excluded from analysis, or with Andamanese if Europeans are excluded from analysis [6]. The impression these studies give is that northern and southern Indians are very similar in their craniometrics, with secondary affinities to Sri Lanka, Andamanese, and southwest Asian crania, regardless of whether differences between populations in cranial size are controlled for [6, 9] or not [10].

Until the mid-2000s, theories on the population history of India distinguished between indigenous and immigrant strata [7, 11-14]. The indigenous stratum supposedly consisted of foragers of "Australoid" or "proto-Australoid" racial affinity, with the "Veddoids" (represented by the Veddas) sometimes recognised as a distinct component. The foragers either survived into recent times as enclaves or else were absorbed during the Holocene expansion of farming populations into India from Central Asia and/or the Mediterranean. Two separate demographic expansions were recognised, one leading to Dravidian speakers in the south and the other to Indo-European (Indo-Aryan) speakers in the north. An additional incursion of farming populations, restricted to northeast India, involved Munda (Austro-Asiatic) speakers with Southeast Asian ("Mongoloid") affinities.

The recent accumulation of genetic evidence for the Late Pleistocene origin of Homo sapiens in Africa has had two main implications for understanding India's population history. The first is to reinterpret India's indigenous stratum as the first wave of Homo sapiens colonists en route from Africa to Eurasia and Australia. The second main implication is to cast doubt on the concept of a Dravidian migration and to interpret any Central Asian genetic affinities in southern India as a knock-on effect following the Indo-European immigration into northern India [1, 15]. In the same vein, Reich et al. recognise a distinction between "Ancestral North Indian" and "Ancestral South Indian" complexes. The former is closely related to Middle East, Central Asian, and European populations whereas the latter has no demonstrable similarities with other Eurasians. Within the Indian subcontinent, Indo-Aryan speakers have predominantly "Ancestral North Indian" ancestry and Dravidian speakers predominantly "Ancestral South Indian" ancestry, while the Onge of the Andaman Islands have retained undiluted "Ancestral South Indian" ancestry [16].

Our paper compares Indian crania with the series recorded by Howells [17] on a large suite of craniometric measurements, to test three hypotheses on Indian affinities arising from recent genetic studies. The first hypothesis is that northern and southern India crania can be more clearly distinguished from each other than earlier craniometric studies have indicated. The second hypothesis is that northern Indian crania will show affinities to Howells' Egyptian and European crania. The third hypothesis is that southern Indian crania will show affinities with Andaman Islander rather than Egyptian and European crania.

2. Materials and Methods

Between 2001 and 2005, the first author measured over 1,300 adult crania held in anatomical institutions across India, along with a small number in Adelaide, Australia. Twelve ethnic groups are represented amongst the cranial collections but two of them, the Coorg and Bengalis, are unfortunately available only as very small sample sizes. Also, the first author did not come across crania from many of the "tribal groups" that would be of potential interest for our study, groups such as the Munda, Santhal, Yanadi, and Irula. Table 1 and Figure 1 present the language, language distribution [18,19], geographic location, and holding institutions of the ten series that could be included in this study.

Most of the measured crania were obtained from medical dissections of adults of known language affiliation, with smaller numbers donated by collectors or recovered from historical gravesite excavations. Over 90 percent are of known adult status and sex, as recorded in the mortuary registers, and some come from named individuals. In addition the first author, often accompanied by senior curatorial staff, inspected the crania for their degree of dental development and cranial suture closure to confirm their adult status, as well as their general size and the robustness of their mastoid process, supraorbital region, and nuchal musculature to confirm their recorded sex. This familiarisation with the morphological variation shown by adult males and females of each Indian series allowed the first author to sex the adult crania of unrecorded sex, assisted by documentation of the pelvis and other postcranial bones where these were available.

Crania whose measurements appeared to be affected by recorded signs of pathology were excluded.

The first author took all 47 craniometric variables defined by Howells [3] excluding his radii (Table 2). He transcribed his measurements and specimen documentation to an Excel spreadsheet, against which the second author checked the original records. The second author also performed logical checks on the data, including calculation of 17 indices (Table 2) that reflect main aspects of cranial shape (wherever the numerator and denominator measurements were both available). Any noted inconsistencies were resolved through mutual agreement. The Supplementary Material presents the sample sizes, means, standard deviations, and ranges for the measurements and indices of the ten series included in this study.

Six analyses are presented in this paper, making use of our large craniometric database, which allows us to exclude any specimens missing the analysed variable or variables. The first deals with intraseries variability, considering both the samples' standard deviation and their range (the difference between the largest and smallest value within the sample). The second analysis focuses on sexual dimorphism and the third investigates craniometric variation within India. Determination of statistical significance in these analyses is based on the weighted Simes test [20]. The fourth analysis compares male average measurements and indices of South Asians, including the Veddas of Sri Lanka based on previously published data [5, 7], with those from other parts of the world. Males rather than females are chosen here because they are better sampled. The fifth and sixth analyses employ multivariate techniques to compare the six best sampled Indian series with the Howells series (males and females). The first of these analyses is a principal components analysis [21], and the second is based on Mahalanobis-D distances calculated from Mosimann indices [22] which are widely used in osteometric studies where the focus of attention is shape [23-26]. All multivariate analyses were undertaken using XLSTAT.

A note of caution arises from the decision by Howells [3] to substitute missing variables in the crania he measured with the average measurement from the series concerned. One obvious effect is to artificially increase the sample size for at least some variables and artificially decrease these variables' standard deviations. These points render Howells' data inappropriate for intraseries variability analysis. As for Howells' estimates of his series' means, included in our fourth analysis, the mean values themselves will not be affected, and therefore indices calculated from his series' means are also not affected. The effects on the fifth and sixth analyses are unknown but probably slight because Howells focused on crania with the great majority or all of their measurements intact.

3. Results

3.1. Analysis of Variability. Variability, as measured both with the standard deviation and the range, tends to increase as the mean increases, for all variables and across all series. However, the standard deviation and range increase at a far slower rate than that of the mean. While the linear relationship between the mean and the standard deviation is moderate (Pearson's r = 0.74), the slope of the best-fit line at 0.03 is flat. Similarly, Pearson's r for the linear relationship between the mean and the range is 0.70, while the slope of the best-fit line is merely 0.14 (Tables 3 and 4).

The association between the mean and variability measures improves markedly by the following two steps, although the slope of the best-fit lines remains flat. The first step is to exclude Howells' parietal, frontal, and occipital fractions on the basis of their excessive variability. Even though these cranial fractions (by definition) are smaller than their respective cranial chords, it is the cranial fractions that generally have the larger standard deviation (Supplementary Tables S3 and S4). When fractions are excluded, Pearson's r correlation coefficient increases to 0.81 comparing means and standard deviations and to 0.75 comparing means and ranges (Tables 3 and 4). The second step is to exclude sample sizes less than 30, based on the rule of thumb that 30 is a sufficiently large sample size to reliably estimate the main parameters of a population [27]. When the smaller samples are excluded, Pearson's r correlation coefficient increases to 0.89 comparing means and standard deviations and to 0.85 comparing means and ranges.

The coefficient of variation, or mean divided by the standard deviation, widely used in biostatistical analyses [28] has been critiqued as not applicable in comparing one variable with another [29]. As the present analysis shows, in the case of craniometric variables, the standard deviation increases with the mean albeit at a far slower rate. In fact, the relationship of the standard deviation and the range to the mean is log-linear rather than linear, as shown by expressing these variables as logarithmic values. Following this transformation to the variables, Pearson's r following this transformation to the variables, is always higher than was the case with the untransformed variables. Also, the slope of the best-fit line is always above 0.4, close to the 0.5 value that would reflect equal rates of increase between the variables being compared (Tables 3 and 4).

The preceding analysis suggests that the range is just as useful as the standard deviation in systematically charting variabilitywithin a cranial series. While the range has a poor reputation for being affected by extreme cases, the point being made here is that extreme cases can be expected for any well-sampled series. For instance, if we consider vault length (GOL) for males and females with a sample size of at least 30, the smallest male value is always less than 162 mm and the largest male value is always greater than 190 mm, while the smallest female value is always less than 159 mm and the largest female value is always greater than 182 mm (Supplementary Table S1). Thus, any temptation to "cleanse" a series by trimming it of specimens with extreme measurements--for instance, measurements more than two standard deviations from the mean--should be avoided, as it would impose an artificial homogeneity on the series. However, where particular variables depart from the general pattern shown by the other variables, as observed here with cranial fractions, these should be removed from analysis as their heightened variability is likely to be an artefact of measurement uncertainty.

Does the intraseries variability noted here primarily reflect differences in size, distinguishing crania with consistently large measurements from crania with consistently smaller measurements? If so, we may expect only a weak correlation between the means of the main indices and their variability. The relationship between the index mean and its variability is difficult to discern when indices are considered as a single set. For instance, Pearson's r for all indices for all series, comparing the index means and standard deviations, is low, at 0.17. However, this lack of a clear positive correlation is entirely due to extreme variability of the two indices that reflect protrusion of the nasal bones (DKB:NDS and WNB:SIS in Supplementary Table S12). (While intraseries variability of the nasodacryal index (DKB:NDS) has not previously been investigated, the high variability of the simotic index (WNB:SIS) has already been noted [5, 8].) When these two indices are excluded, index means show a moderate positive correlation with standard deviations and ranges (Table 5), as high as 0.78 (log-transformed index and standard deviation for all series with a sample size of at least 30). Generally speaking, indices resemble measurements in the degree to which variability scales with mean values, as would be consistent with considerable intraseries shape variability.

The extent and ubiquity of shape variation within Indian cranial series can be shown by considering index ranges (Supplementary Tables S1 to S10) in terms of the standard index categories used in physical anthropology [30]. All series except the Konkani include both hyperdolichocephalic crania with a cranial index less than 65 and brachycephalic crania with a cranial index above 80. All series other than the Konkani include chamaecranic individuals with a vault length-height index less than 70 and hyperhypsicranic individuals with a vault length-height index above 80. Similarly, the upper facial index ranges from hypereuryenic individuals (index less than 45) to lepten individuals (index above 55) in every series other than the Urdu. In every series, the orbital index ranges between chaemoconchic (less than 76) and hypsiconchic (above 85), and the nasal index ranges between leptorrhine (less than 47) and hyperchamaerrhine (above 58). Similarly, the frontal curvature index breakpoints proposed by Larnach and Macintosh [31] do not begin to capture the variability recorded for Indians. Every series includes individuals with very receding frontals as shown by an FRC:FRS index less than 21 and individuals with very bulging frontals as shown by an index above 27.

Although many of the following comparisons in this paper focus on series means, the results should not be interpreted in a typological sense, given the demonstration of how variable crania are within any Indian series.

3.2. Sexual Dimorphism. The generally larger size of male compared to female crania, well established for populations worldwide, applies to Indians too. One way to illustrate this pattern is to divide the male average by the female average for the ten recorded Indian series, for each measurement, and present the resulting ratios as percentages (Figure 2). There are a few measurements where the male average is proportionately much larger than the female average, notably glabella subtense (GLS, around 150-200%, depending on series), supraorbital subtense (SOS, around 120-170%), and the dimensions of the mastoid process (MDB and MDH, around 110-150%). At the other end of the scale, some measurements show minimal sexual dimorphism, notably foramen magnum length (FOL), orbital height (OBH), and frontal subtense (FRS). By and large, however, there is a tendency for male averages to cluster at around 110% of the corresponding female averages.

Accordingly, the size of the difference between male and female averages largely reflects whether the measurement is big or small. If we subtract the female from the male average and correlate the result with the male average, excluding fractions and series with a sample size of less than 30 for both males and females, we find a Pearson's r correlation coefficient of 0.773. (Investigation of the correlation between these variables after log transformation is not possible because, as shown in Figure 2, the female average subtracted from the male average occasionally yields zero or a negative number, neither of which can be log-transformed.)

Another observation to be inferred is that the measurements with the greatest sexual dimorphism, as reflected by the average female: male ratio, also tend to be small (GLS to NLH in Figure 2). Small measurements are also distinguished from large measurements by a greater standard deviation in relation to the mean (Table 3), which makes the proportionate relationship between the male and female means an unreliable predictor of whether or not there is a statistically significant difference between the male and female means. This is demonstrated in Table 6, which presents an analysis in terms of the series for which males are significantly larger than females (one-tailed Student's t-test, P set at 0.05 or a smaller number as required by the weighted Simes test).

At one extreme are six measurements significantly larger for males than females in all ten Indian series. At the other extreme are four measurements that are not significantly larger for males than females in any of the series (including FRS, which is actually larger for females than males in all Indian series with a male sample size of at least 30). In between are 16 measurements significantly larger for males than females as long as the male sample size is at least 30; 11 measurements significantly larger for the clear majority of series with a male sample size of at least 30; and eight measurements with weak sexual dimorphism. These intermediate cases include all four measurements (GLS, SOS, MDB and SIS) with the most pronounced sexual dimorphism based on the proportionate comparison of the male and female means.

The ordering of series in Table 6 shows the importance of adequate sample size to identify sexual dimorphism in human cranial measurements. The Tulu, Haryanavi, Telugu, Punjabis, Kannada, Tamils, and Hindis, with a minimum sample size of at least 30 for males (and 18 for either sex), all show males to be significantly larger than females for around 60-80% of measurements. In contrast, the Malayalam, Konkani, and Urdu, represented by smaller sample sizes, can be shown to be sexually dimorphic for just 20-30% of measurements.

For most indices, males and females from the same Indian series show very similar average values (Supplementary Tables S1 to S12), especially where sample size is large enough to be reliable (as for the bottom seven series in Table 6). However, there are several indices where males and females consistently differ. Average frontal curvature index (FRC:FRS) is larger for females than males in the same series, to a degree that is statistically significant as long as male sample size is at least 30. This accords with the recognition that male frontals tend to slant back more strongly compared to females' more rounded frontals [32]. Females exceed males from the same series in their average orbital index (OBB:OBH), to a statistically significant degree in the case of Hindis, Kannada, Tamils, Telugu, Tulu, and Konkani. This is consistent with the recognition that females tend to have a more rounded upper orbital margin than males [33]. On the other hand, males' XCB:ZYB index consistently exceeds that of females in the same series, significantly so for Hindis, Kannada, Tamils, Tulu, and Urdu, related to the presence of a wider zygomatic arch as a male marker for the human skull [32]. Finally, males tend to have a more prominent nasal skeleton than females from the same series. This is reflected by males' significantly larger nasodacryal or DKB:NDS index for Hindis, Haryanavis, and Telugu and larger simotic (WNB:SIS) index for Hindis, Tamils, and Telugu. These instances of sexual dimorphism in cranial shape suggest a potential shortcoming in multivariate statistical studies [6,9] that pool males and females in the same analysis, attempting to accommodate sexual dimorphism by simply compensating for cranial size.

3.3. Craniometric Variability within India. For most measurements and indices, when the means are considered, consistent differences between northern and southern Indians, or between Indo-Aryan and Dravidian speakers, are difficult to discern. For instance, the range of means for cranial length is 176.5-181.9 mm for northern Indian males and 176.1-181.9 mm for Indo-Aryan males, which overlap extensively with the range of means of 173.0-178.6 mm for southern Indian males and 173.0-178.5 mm for Dravidian males (Supplementary Table S1). Similarly, looking at cranial index we find little if any difference between northern Indian males (70.7-72.3) and southern Indian males (71.3-73.0) or between Indo-Aryan males (70.7-72.2) and Dravidian males (71.3-73.0).

There are, however, some differences between northern and southern Indians in their craniometrics, comparing males with males and females with females, to a degree that is statistically significant, generally speaking (weighted Simes test). Average supraorbital projection (SOS) is larger for northern Indians than southern Indians (Table 7 and Supplementary Table S8). The orbits are on average narrower amongst northern Indians than southern Indians (Table 7 and Supplementary Table S9), whether expressed in terms of their smaller orbital breadth (OBB) or higher orbital index (OBB:OBH). On the other side of the ledger, northern Indian interorbital breadth (DKB) tends to be larger than southern Indian interorbital breadth (Table 7 and Supplementary Table S10). As for facial flatness, northern Indians' dacryon subtense (DKS) is on average smaller than southern Indians', whereas their zygomaxillary subtense (SSS) is relatively large (Table 7 and Supplementary Table S11).

In the preceding comparisons, statistically significant differences between northern and southern Indians are clearer for male than female comparisons. This point applies with even greater force to the two identifiable north-south differences in vault metrics. Male Hindis and Haryanavis have a lower vault length-height index than all southern Indians males, but this difference is less clear when females are compared (Table 7 and Supplementary Table S1). (The average vault length-height index of Punjabi males is also lower than that of any southern Indian male series, but the difference is statistically significant only in comparison with Konkani males; and with the females, Punjabis actually have a significantly higher index than the Malayalam.) Also, northern Indian male frontals tend to be narrower than southern Indian male frontals, as shown by the smaller bistephanic breadth (STB) and maximum frontal breadth (XFB) of northern Indian males (Table 7 and Supplementary Table S3). However, the only reflection of this difference in the female comparisons is female Hindis' significantly smaller XFB compared with the XFB of Telugu, Kannada and Tamil females.

3.4. Male Indian Averages Compared to Other Series. To place the craniometric differences between northern and southern Indians in context, this section compares the averages for male Indians with the averages recorded for other series, notably by Howells [17] but also byWarusawithana-Kutilake [7] for Veddas, supplemented by Veddas' simotic index from Woo and Morant [5].

Figure 3 focuses on six main cranial measurements in showing that Indian crania are small by general standards. Indians' breadth measurements are amongst the smallest in the world, and their length measurements and nasionprosthion height are below average, although their basionbregma height is moderate. The small size of Indian crania can be related to Indians' generally small body size [34]. Two pygmy populations, the Andamanese and Kalahari Bushmen, have crania that are usually smaller than Indians' except on breadth measurements. The Veddas, who are also smallbodied, have average cranial measurements that either fall within the Indian range or, in the case of nasion-prosthion height, below it.

Figure 4 compares Indians with other series on six indices that have cranial chords as the denominator. Indian cranial vaults are on average narrow (dolichocranic) but relatively tall, at least in the case of southern Indians, and have welldeveloped frontal curvature but variable parietal and occipital curvature. Indian crania also tend to be orthognathic, with basion-nasion length greater than basion-prosthion length. Veddas consistently fall within the Indian range, but no other series shows the suite of features displayed by Indians. For instance, Southwest Pacific ("Australoid") series resemble Indians in their narrow vault but differ in their generally lower vault, prognathism, more receding frontal, and more bulging occipital bone.

Figure 5 compares Indians with other series on six indices that involve facial chords. Indian crania on average have a moderately wide biasterionic breadth in relation to bizygomatic breadth. Stock et al. [9] also found this to be a feature of Indian crania, but in their analysis it was a feature otherwise shared with Andamanese and Veddas, whereas here we instead find a European/Egyptian ("Caucasoid") similarity for Indians. Indian crania also have a moderate transverse craniofacial index, narrow face, wide bimaxillary breadth compared to bizygomatic breadth, quite narrow nasal aperture, and variable orbit shape. Indians' variable orbit shape reflects the difference between southern Indians with broad orbits and northern Indians with narrower orbits, noted above. None of the comparative series show the suite of features displayed by Indians. For instance, Caucasoids resemble Indians in their moderately wide biasterionic breadth and narrow faces, but differ in their lower transverse craniofacial index and narrower bimaxillary breadth and nasal aperture.

Figure 6 compares Indians with other series on their facial flatness indices. A low index reflects a flat face, as shown particularly by the Buriats, other East Asians, and Kalahari Bushmen, whereas a high index reflects a medially protrusive face. Indians are shown to have faces that are medially very protrusive across the frontal, orbital, and nasal regions and moderately protrusive across the maxilla (lower face). Veddas fall within the Indian range on the three available comparisons. Comparable results were obtained by Woo and Morant [5] and Hanihara [8], who found that South Asians tend to have the highest frontal flatness index in the world, high simotic index, and moderate (zygo/pre) maxillary index. The larger dacryon subtense of southern Indians compared to northern Indians, noted above, is underlined by southern Indians' particularly high orbital flatness index.

Table 8 summarises the index comparisons in Figures 4 to 6.WhenIndians arecomparedtoother groups representedby more than one series, a similarity is recognised when Indians either fall within the range of the other group or else encompass the range of the other group. Dissimilarity is recognised when the ranges are mutually exclusive. However, when Indians are compared to single series, such as Andamanese or Veddas, similarity is recognised when that series falls near the centre of the Indian range, and dissimilarity is recognised when it falls well away from the Indian range.

Except for Buriats, every group or single series is similar to Indians on at least one index. Europe, Veddas, and Egypt register the largest number of similarities (resp., seven, six, and five). Veddas are dissimilar from Indians on just one index, but every other group or single series is dissimilar on between four and six (Ainu, Egypt, Europe) to 13 indices (Kalahari Bushmen). Veddas would appear to be the nonIndian series most similar to Indians, followed by Caucasoids.

3.5. Principal Components Analysis (PCA). PCA provides a multivariate perspective on the univariate and bivariate comparisons detailed above. The comparisons include all of the better sampled series measured by Howells [17] but exclude the Veddas, for whom we lack access to the original measurements. The six Indian series with the largest sample size--three from northern Indian and three from southern India--are included for analysis. As noted above, northern Indian cranial series are very similar to each other, as are southern Indian cranial series. Therefore, including the less well sampled Indian series would just add noise to the analysis. Further, males and females are analysed separately in view of their shape distinctions, as described previously. In addition, cranial chord fractions are excluded in view of their unreliability as reflected by their excessive variability.

Application of PCA produces very similar results for both males and females. The first component (PC1) accounts for 30% of variability (approximately), the second and third components (PC2 and PC3) for 8% each, and the fourth and fifth components for 5% each (Table 9), with decreasingly smaller amounts for the remaining components. As is common when PCA is applied to biological data [21], PC1 is a size component, with positive weightings on most variables (Table 10). In the present analysis, however, the PC1 weightings for the upper and middle facial subtenses are either negative (NAS, DKS, and SIS) or weakly positive (NDS). That is, large overall cranial size tends to be associated with upper and middle facial flatness. These subtenses also have the highest positive loadings on PC2, followed by cranial lengths, while minimum cranial breadth (WCB), maximum cranial breadth (XCB), and malar subtense (MLS) have the strongest negative loadings. In the case of PC3, which is the second most important of the shape components, cranial breadths and especially frontal breadths have the highest positive loadings (STB, XFB, XCB and AUB), while basion-prosthion length (BPL) has a strong negative loading (Table 10).

Figure 7 illustrates how the different series score on PC1, arranged in approximate order from the series with the largest crania (Buriats, Guam, Polynesians, Eskimos, and the Ainu of Japan) to the series with the smallest crania (Indians, Kalahari Bushmen and Andamanese). Figure 7 presents the range of PC1 scores for each series as well as the series averages. Inspection of the ranges shows that the smallest specimens in the series with the largest crania are of approximately the same size as the largest specimens in the series with the smallest crania. In the case of the series with intermediatesized crania, their ranges overlap extensively with the ranges of both the series with large and small crania. Also of interest, there is a difference of around 10 between the series with the largest and the smallest crania in terms of their average PC1 score (Buriats scoring just over 5 compared with Kannada scoring just over -5), and this is similar to the difference of around 10 between the largest and smallest crania within any series (for instance, Buriats ranging between about 10 and 0, and Kannada ranging very approximately between 0 and -10). These observations apply equally to males and females.

Figures 8 and 9 illustrate how the series compare the two main shape components, PC2 and PC3. The series are represented both by their centroids and their ranges of variation. For most series, these ranges overlap extensively and so it would be very difficult, and arguably unnecessary, to distinguish them from each other. The only ranges that can be individually labelled are those that relate to series that fall towards the extremes of modern human craniometric variation. Both the centroids and the ranges carry the same message for understanding differences between series in cranial shape. For instance, Buriats are distinguished by a strongly negative score on PC2 and a strongly positive score on PC3. This is shown by the position of the Buriat centroid and also by the Buriat range, with approximately half of the Buriat range of variation falling outside the range of variation documented for any of the other series. Also, while most series overlap with Buriats on their range, some do not, notably Southwest Pacific groups (Australians, Tasmanians, and Tolai), Easter Islanders, and (in the female analysis) the Teita and Zulu from Sub-Saharan Africa.

In accord with the index analysis described above, Indians and Caucasoids align on the two main shape indices. Their centroids are neutral to weakly positive on both PC2 and PC3, and their ranges include the only analysed crania that are strongly positive on both of these PCs (Figures 8 and 9). Their centroids are distant from the Southwest Pacific centroids and, to a lesser degree, the Teita and Easter Island centroids, which are strongly negative on PC3. The Indian and Caucasoid centroids are also quite distant from the Andamanese and Bushman centroids, which are weakly negative on both PC2 and PC3. However, a point of interest is that the Ainu centroid is close to the Indian and Caucasoid centroids on both the male and female analyses.

The graphical representation of the PC2 and PC3 results is of value in reiterating certain observations that emerged from the index analysis, notably the general similarity between Indians and Caucasoids, especially in sharing a medially protrusive face. It is also of value in indicating a central range of human craniometric shape variation, where most of the series comprehensively overlap with each other and where most of the centroids lie. It is however limited in its value in representing only some 16% of craniometric variability. To obtain a more complete picture of the circa 70% of human craniometric variability that is shape rather than size related, we turn to Mahalanobis D-distance comparisons of Mosimann indices.

3.6. Mahalanobis-D Distances Comparisons of Mosimann Indices. Mosimann indices control for size by dividing a specimen's measurements by the geometric mean of all of its measurements [22]. Supraorbital and glabella projection need to be excluded from analysis, as they can measure zero on crania from India (and elsewhere), which prevents calculation of the measurements' geometric mean. Once the measurements were transformed into Mosimann indices, the Mahalanobis [D.sup.2] distances between series were calculated. These distances were then converted into Euclidean distances by calculating their square roots (Mahalanobis-D distances). The series were then clustered using average-linkage hierarchical clustering.

Both the male and female dendrograms were seriated, according to a procedure described elsewhere [35]. This procedure involves placing the series and/or clusters most different from each other at opposite poles of the seriation, and, to the extent permitted by the structure of the dendrogram, ordering the other series and/or clusters according to the degree to which they approach one or the other pole. In addition to emphasising extreme differences, the seriation exercise allows for the representation of secondary affinities that may not be captured by the clusters themselves. The success of the seriated order in producing a perfect seriation is measured by the coefficient of variation between the ordered matrix of interseries distances and the closest matrix that could be found with all of the distances perfectly seriated. (The results obtained here, which fall between 70 and 75%, can be described as "fair.")

Based on the preceding analyses, the following predictions can be made of how Indians should compare with the series measured by Howells. Indians should form a distinct cluster, albeit with northern Indians distinguishable from southern Indians. Indians should be approached by Caucasoids, whereas Buriats and Bushmen should be far removed.

The seriated dendrograms (Figures 10 and 11) agree in certain fundamental respects. The three northern Indian series cluster together, as do the three southern Indian clusters, before joining together into a distinct Indian cluster. Also, southern Indians lie at one pole, whereas northern Indians are intermediate between southern Indians and other series (see below). In addition, Egyptians join the three European series to make a Caucasoid cluster, while the Andamanese join the Dogon, Teita, and Zulu of Sub-Saharan Africa to form a distinct cluster. Another similarity between the male and female analyses is that Buriats, Eskimos, Easter Islanders, and Southwest Pacific series (Australians, Tasmanians, and Tolai) are the series most distant from Indians.

Where Figures 10 and 11 disagree is in the secondary affinity suggested for Indians. In the male analysis, Indians are flanked by Sub-Saharan African series (here counting Bushmen), whereas in the female analysis, Indians plot adjacently to Caucasoids. Inspection of the original distances (Tables 11 and 12) shows that the female analysis is the more informative from the perspective of revealing Indians' wider affinities. Both male and female Indians are closer to Europeans and Egyptians than to any other analysed series, notably Bushmen. The reason why this fact does not emerge from the male analysis (Figure 10) is because the craniometric distances between Indians and Sub-Saharan Africans are smaller for males than females (Tables 11 and 12). Accordingly, the male seriated dendrogram emphasises the craniometric distance between Buriats/Eskimos and SubSaharan Africans, overriding the craniometric difference between Indians and Sub-Saharan Africans, whereas in the female analysis these emphases are reversed.

The similarity between Indian and Andamanese crania proposed by previous multivariate studies [6, 9, 10] cannot be confirmed by our analysis. Instead, Andamanese cluster with Sub-Saharan Africans, as originally observed by Howells [3], whereas Indians are more similar to Caucasoids than to any other populations outside of South Asia. The reason for the difference between our findings and those of previous studies on Indian craniometrics may be the larger number of variables in our analysis presented here, 42 compared with 20 to 30. Further, there is no evidence for the southern IndianAndamanese affinity that would have been expected from the genetic comparisons of Reich et al. [16]. With barely an exception, southern Indians register a greater craniometric distance from Andamanese than northern Indians do, just as southern Indians are more distant than northern Indians

4. Discussion

The literature review in our Introduction generated three hypotheses for our craniometric analysis. The first hypothesis, the distinction between northern and southern Indians within a discrete Indian cluster, was unequivocally confirmed. The second hypothesis was confirmed as a secondary "Caucasoid" affinity emerged for northern Indians. However, the expectation from the third hypothesis of a secondary Andamanese affinity for southern Indians was falsified.

The craniometric differences found here between northern and southern Indians are not in terms that might have been expected from the comparative literature. For instance, based on Bharati et al. [36], narrower crania might have been expected in the south than the north, but instead all of the sampled Indian series were found to have similarly narrow crania. The explanation for this result maybe that all of these Indian populations inhabit hot climates [37], even if the torrid heat of northern India's lowlands is a seasonal phenomenon. If there is an adaptive basis for the differences that southern Indians show from northern Indians, such as broader orbits that are medially more protrusive, this basis remains to be explored.

The distinctiveness of Andamanese and southern Indian crania need not challenge the finding by Reich et al. [16] for an "Ancestral South Indian" ancestry shared by southern Indians and Andamanese. The point is that some populations are craniometrically specialised while others are not. The specialised nature of Buriat craniometrics, which is very clear both from index and multivariate analysis (Figures 4 to 11), has been noted previously [6]. What the present analysis adds is that southern Indians also have specialised craniometrics. Andamanese on the other hand have unspecialised craniometrics, as shown by how they cluster with geographically distant Sub-Saharan Africans, and seriate adjacently to the central bloc of recent human crania (consisting of Caucasoids, Amerindians, and populations from Japan and China to Taiwan and parts of the Pacific). Therefore, southern Indians' craniometric distinctiveness from Andamanese should be interpreted as a result of their craniometric specialisation rather than as evidence against a shared, ancient ancestry with Andamanese.

Populations with medially protrusive upper and middle faces are distributed from Scandinavia to the circum-Mediterranean, India, and Sri Lanka [5, 8]. This indicates the existence of a population complex extending from Scandinavia south-south-east to Sri Lanka. Gene flow across this continuously populated region would have been relatively uninterrupted in comparison to the formidable barriers to gene flow presented by the Atlantic Ocean to the northwest, the Sahara Desert to the southwest, and the Himalayas and Eurasian Steppe to the northeast. Upper and middle facial protrusion are developed particularly strongly in southern India (Figure 6). This observation is not explicable in terms of a contribution to the southern Indian gene pool from Central Asia and/or the Mediterranean. On the other hand, the intermediate position of northern Indians between southern Indians, and Caucasoids northwest of India, could be explicable in terms of the incursion of Indo-European (IndoAryan) speakers into northern India from the northwest, or alternatively it could simply reflect clinal variation.

If there were an Australoid "substratum" component to Indians' ancestry, we would expect some degree of craniometric similarity between Howells' Southwest Pacific series and Indians. But in fact, the Southwest Pacific and Indian are craniometrically very distinct, falsifying any claim for an Australoid substratum in India. Only the "Veddoid" substratum component invoked by some theories would be potentially supported, based on the index similarities between Veddas and Indians. The problem with this proposal is that, craniometrically, the Veddas should be viewed as just another South Asian population. The basis for invoking the Veddas as representative of a substratum component, rather than the Kannada and Tamils (for instance), appears difficult to justify. Noteworthy in this context is the accumulating evidence that the Dravidian languages have a preagricultural origin in southern India and dispersed with the expansion of the southern Indian Neolithic [38].

We recognise that craniometric data are not as powerful as genetic data in unmasking populations' biological relatedness. Indeed, where our results could not duplicate the affinity between southern Indians and Andaman Islanders suggested by genetic data, we attributed the discrepancy to southern Indians' craniometric specialisation. However, craniometric data have particular value for the analysis of ancient South Asian burials [13] which may not support genetic analysis if none of their ancient DNA has survived. As documented here, South Asian crania are characterised by a distinctive suite of features which include tall, narrow vaults, and medially protrusive upper and middle faces. The hypothesis of predominantly local genetic origins for South Asian populations predicts that these features will also characterise prehistoric burials. This point provides direction on where to focus attention in future studies on India's prehistoric burials. At the same time, we should be mindful of how variable crania within any Indian series can be in their shape, an aspect that presumably also applied in the past. Therefore, when analysing a single specimen, we should be duly cautious in how much weight to place on the outcome, and when analysing a series we should expect some healthy variability in the results.

5. Conclusions

Craniometric variability within any Indian series is considerable, but between Indian series it is slight for most measurement and indices. Craniometric differences within India boil down to a primary distinction between northern and southern Indians. When crania from outside India are considered, the Veddas are similar enough to be grouped with Indians as "South Asians" Otherwise, Caucasoid series from Egypt and Europe are closest to Indians, especially northern Indians. The similarity between these Caucasoids and northern Indians would be expected from geographical considerations, but it may also reflect some degree of population incursion into northern India associated with the introduction of Indo-Aryan languages. Southern Indians have specialised craniometrics otherwise revealed only by other South Asians. Craniometric analysis thus accords with recent genetic studies that point to a predominantly indigenous component in Indians' biological ancestry.


Funding for the first author's fieldwork was provided by two sources. One was a large Australian Research Council grant to the second author and Colin Groves ("The Contribution of South Asia to the Peopling of Australasia") and the second was a grant to the first author from the Australia-India Council in Canberra. The authors would also like to thank the many postgraduate medical students and researchers from various institutions who assisted the first author with access to the collections, especially the curators and heads of the collections under their care: Professors Balbitr Singh of the Government Medical College, Chandigarh; Professors Subash Kaushal of the Government Medical College, Patiala; Professors Moly Paul of Christian Medical College, Ludhiana; Professors Reva Chaudhary of Lady Harding Medical College, New Delhi; Professors Kaul of Maulana Azad Medical College, New Delhi; Professors Navneet Chauhan of King George Medical College, Lucknow; Professors Sahai of the Department of Anthropology, University of Allahabad; Professors D. K. Satpathy, Director of the Medico-Legal Institute, Bhopal; Professors Usha Dhall of the Post Graduate Institute of Medical Sciences, Rothak; Professors Ram Narayanan of Manipal Medical College, Karnataka; Professors Nageswara Rao of Kasturba Medical College, Mangalore; Prof Sarada Devi and Dr. Teegala M. Reddy of Osmania Medical College, Hyderabad; Professors Subhadra Devi and Professors Papa Rao of Sri Venkateshvara University, Tirupathi; Dr. Kishore Kumar of Government Veterinary College, Vijayawada; Professors Balasubramaniam of St. John's Medical College, Bangalore; Dr. Manoj Kumar Mohanty of Dr. PNS Institute of Medical Sciences & Research Foundation, Krishna; Professors Padmanaban of the Post Graduate Institute of Medical Sciences, Pondicherry; Professors James Pandiyan and I. Jeya Raj of Madras Medical College, Chennai; Professors Geeta Ramesh of the Madras Veterinary College, Chennai; and Dr. Barry Craig at the Anthropology Department of the Museum of South Australia, Adelaide. Finally, the authors thank two anonymous referees for their remarks on an earlier version of this paper.


[1] G. ArunKumar, D. F. Soria-Henanz, V. J. Kavitha et al., "Population differentiation of southern Indian male lineages correlates with agricultural expansions predating the caste system," PLoS ONE, vol. 7, no. 11, Article ID e50269, 2012.

[2] S. Oppenheimer, Out of Eden: The Peopling of the World, Robinson, London, UK, 2004.

[3] W. W. Howells, Cranial Variation in Man: A Study by Multivariate Analysis of Patterns of Difference among Recent Human Populations, vol. 67 of Papers of the Peabody Museum, Harvard University, Cambridge, Mass, USA, 1973.

[4] G. Harrower, "A study of the Hokien and the Tamil skull," Transactions of the Royal Society of Edinburgh, vol. 54, no. 3, pp. 573-599, 1926.

[5] T. L. Woo and G. M. Morant, "A biometric study of the "flatness" of the facial skeleton in man," Biometrika, vol. 26, pp. 196-250, 1934.

[6] C. L. Brace, D. P. Tracer, and K. D. Hunt, "Human craniofacial form and the evidence for the peopling of the Pacific," Bulletin of the Indo-Pacific Prehistory Association, vol. 11, pp. 247-269, 1991.

[7] S. Warusawithana-Kutilake, Cranial Variation and the Dispersal of Modern Humans in South Asia, Tharansee Prints, Colombo, Sri Lanka, 1996.

[8] T. Hanihara, "Frontal and facial flatness of major human populations," American Journal of Physical Anthropology, vol. 111, pp. 105-134, 2000.

[9] J. Stock, M. M. Lahr, and S. Kulatilake, "Cranial diversity in South Asia relative to modern human dispersals and global patterns of human variation," in The Evolution and History of Human Populations in South Asia, M. D. Petraglia and B. Allchin, Eds., pp. 245-268, Springer, New York, NY, USA, 2007.

[10] R. Wright, "Detection of likely ancestry using CRANID," in Forensic Approaches to Death, Disaster and Abuse, M. Oxenham, Ed., pp. 111-122, Australian Academic Press, Bowen Hills, Australia, 2008.

[11] C. S. Coon, The Origin of Races, A. Knopf, NewYork, NY, USA, 1962.

[12] M. K. Bhasin, H. Walter, and H. Danker-Kopfe, People of India: An Investigation of Biological Variability in Ecological, Ethno-Economic and Linguistic Groups, Kamila-Raj Enterprises, Delhi, India, 1994.

[13] K. A. R. Kennedy, God-Apes and Fossil Men: Paleoanthropology of South Asia, The University of Michigan Press, Ann Arbor, Mich, USA, 2000.

[14] P. Bellwood, First Farmers: The Origins of Agricultural Societies, Blackwell Publishing, Malden, Mass, USA, 2005.

[15] P. P. Majunder, "The human genetic history of South Asia," Current Biology, vol. 20, pp. R184-R187, 2010.

[16] D. Reich, K. Thangaraj, N. Patterson, A. L. Price, and L. Singh, "Reconstructing Indian population history," Nature, vol. 461, no. 7263, pp. 489-494, 2009.

[17] The William W. Howells craniometric data set, 2009, http ://

[18] S. K. Chatterji, Languages and Literatures of Modern India, Prakash Bhavan, Kolkata, India, 1963.

[19] A. Sarker, Handbook of Languages and Dialects of India, Firma K. L. Mukhopadhyay, Kolkata, India, 1964.

[20] F. Bretz, M. Posch, E. Glimm, F. Klinglmueller, W. Maurer, and K. Rohmeyer, "Graphical approaches for multiple comparison procedures using weighted Bonferroni, Simes, or parametric tests," Biometrical Journal, vol. 53, no. 6, pp. 894-913, 2011.

[21] I. T. Joliffe, Principal Components Analysis, Springer, New York, NY, USA, 2nd edition, 2002.

[22] J. N. Darroch and J. E. Mosimann, "Canonical and principal components of shape," Biometrika, vol. 72, no. 2, pp. 241-252, 1985.

[23] A. B. Falsetti, W. L. Jungers, and T. M. Colle III, "Morphometrics of the callitrichid forelimb: a case study in size and shape," International Journal of Primatology, vol. 14, no. 4, pp. 551-572, 1993.

[24] W. L. Jungers, A. B. Falsetti, and C. E. Wall, "Shape, relative size, and size-adjustments in morphometrics," American Journal of Physical Anthropology, vol. 38, supplement 2, pp. 137-161, 1995.

[25] P. J. Obendorf, C. E. Oxnard, and B. J. Kefford, "Are the small human-like fossils found on Flores human endemic cretins?" Proceedings of the Royal Society B, vol. 275, no. 1640, pp. 1287-1296, 2008.

[26] N. von Cramon-Taubadel and R. Pinhasi, "Craniometric data support a mosaic model of demic and cultural neolithic diffusion to outlying regions of Europe," Proceedings of the Royal Society B, vol. 278, no. 1720, pp. 2874-2880, 2011.

[27] T. S. Constandse-Westermann, Coefficients of Biological Distance, Humanities Press, Oosterhout, The Netherlands, 1972.

[28] L. Tian, "Inferences on the common coefficient of variation," Statistics in Medicine, vol. 24, no. 14, pp. 2213-2220, 2005.

[29] W. W. Howells, "Some uses of the standard deviation in anthropometry," Human Biology, vol. 8, pp. 592-600, 1936.

[30] G. Brauer, "Osteometrie," in Wesen und Methoden der Anthropologie, Band I. 1. Teil: Wissenschaftstheorie, Geschichte, morphologische Methoden, R. Knufiman, Ed., pp. 160-192, Gustav Fischer, Stuttgart, Germany, 1988.

[31] S. L. Larnach and N. W. G. Macintosh, The Craniology of the Aborigines of Coastal New South Wales, Oceania Monographs no. 13, The University of Sydney, Sydney, Australia, 1966.

[32] D. Shier, J. Butler, and R. Lewis, Hole's Human Anatomy and Physiology, chapter 7, McGraw-Hill, New York, NY, USA, 11th edition, 2009.

[33] D. R. Brothwell, Digging up Bones, British Museum (Natural History), London, UK, 3rd edition, 1981.

[34] H. Field, Contributions to the Physical Anthropology of India, Field Research Projects, Coconut Grove, Fla, USA, 1970.

[35] D. Bulbeck, "Biological and cultural evolution in the population and culture history of Malaya's anatomically modern inhabitants," in Dynamics of Human Diversity: The Case of Mainland SoutheastAsia, N. Enfield, Ed., Pacific Linguistics no. 627, pp. 207-255, The Australian National University, Canberra, Australia, 2011.

[36] S. Bharati, S. Som, P. Bharati, and T. S. Vasulu, "Climate and head form in India," American Journal of Human Biology, vol. 13, no. 5, pp. 626-634, 2001.

[37] K. L. Beals, "Head form and climatic stress," American Journal of Physical Anthropology, vol. 37, no. 1, pp. 85-92, 1972.

[38] D. Q. Fuller, "Non-human genetics, agricultural origins and historical linguistics in South Asia," in The Evolution and History of Human Populations in South Asia, M. D. Petraglia and B. Allchin, Eds., pp. 393-443, Springer, NewYork, NY, USA, 2007.

Pathmanathan Raghavan, (1) David Bulbeck, (2) Gayathiri Pathmanathan, (3) and Suresh Kanta Rathee (4)

(1) School of Archaeology and Anthropology, College of Arts and Social Sciences, The Australian National University, Canberra, ACT 0200, Australia

(2) Department of Archaeology and Natural History, College of Asia and the Pacific, The Australian National University, Canberra, ACT 0200, Australia

(3) Department of Anthropology, Panjab University, Chandigarh IN-CH160001, India

(4) Department of Anatomy, Post Graduate Institute of Medical Sciences, Rohtak IIM-R124001, India

Correspondence should be addressed to David Bulbeck;

Received 11 July 2013; Revised 13 October 2013; Accepted 15 October 2013

Academic Editor: Shozo Yokoyama

TABLE 1: Indian series included in the present study.

Series         Location        Language    Holding institutions

Punjabi     Northwest India   Indo-Aryan   Panjab: Government
                                           Medical College,
                                           Patiala; Christian
                                           Medical College,
                                           Government Medical
                                           College. New Delhi:
                                           Mulana Azad Medical

Haryanavi   Northwest India   Indo-Aryan   Haryana: Post
                                           Graduate Institute
                                           of Medical Sciences,
                                           Rohtak. New Delhi:
                                           Maulana Azad Medical
                                           College, Lady
                                           Harding Medical
                                           College. Uttar

Hindi         North India     Indo-Aryan   Pradesh: King George
                                           Medical College,
                                           Lucknow; University
                                           of Allahabad,
                                           Allahabad; Moti Lal
                                           Nehru Medical
                                           College, Allahabad.
                                           Madhya Pradesh:
                                           Gandhi Medical
                                           College, Bhopal.

Urdu          South India     Indo-Aryan   Andhra Pradesh:
                                           Osmania Medical
                                           College, Hyderabad;
                                           Gandhi Medical

Konkani       South India     Indo-Aryan   Karnataka: Kasturba
                                           Medical College,
                                           Manipal; Kasturba
                                           Medical College,

Telugu        South India     Dravidian    Andhra Pradesh:
                                           Osmania Medical
                                           College, Hyderabad;
                                           Gandhi Medical
                                           Secunderabad; Sri
                                           Tirupathi; Siddarth
                                           Medical College,
                                           Vijayawada, Andhra
                                           Pradesh; NRI Medical
                                           Chinakakani; DR.
                                           PSIMS & Rf, Krishna.
                                           Adelaide: South
                                           Australian Museum.

Kannada       South India     Dravidian    Karnataka: St John's
                                           Medical College,
                                           Bangalore; Kasturba
                                           Medical College,

Tulu          South India     Dravidian    Karnataka: Kasturba
                                           Medical College,
                                           Manipal; Kasturba
                                           Medical College,

Tamil         South India     Dravidian    Pondicherry:
                                           Jawaharlal Nehru
                                           Institute of Medical
                                           Education and
                                           Research. Tamil
                                           Nadu: Madras Medical
                                           College, Chennai;
                                           Christian Medical
                                           College, Vellore.
                                           Adelaide: South
                                           Australian Museum.

Malayalam     South India     Dravidian    Kerala: Government
                                           Medical College,

TABLE 2: Measurements and indices included in this study.

Measurement/index                           Acronym

Maximum glabello-occipital cranial            GOL

Maximum nasio-occipital cranial length        NOL

Basion-nasion (cranial base) length           BNL

Basion-bregma cranial height                  BBH

Maximum transverse cranial breadth            XCB
(above the supramastoid crests)

Maximum transverse frontal breadth            XFB

Bistephanic breadth (frontal breadth          STB
across the inferior temporal lines)

Bizygomatic facial breadth                    ZYB

Biauricular breadth (across the roots of      AUB
the zygomatic processes)

Minimum cranial breadth (across the           WCB
infratemporal crests)

Biasterionic (maximum occipital) breadth      ASB

Basion-prosthion (facial) length              BPL

Nasion-prosthion (upper facial) height        NPH

Nasal height                                  NLH

Orbital height (left)                         OBH

Orbital breadth from dacryon (left)           OBB

Bijugal breadth (breadth across the           JUB
middle malars)

Nasal breadth                                 NLB

External palate breadth                       MAB

Mastoid process height                        MDH

Mastoid process breadth                       MDB

Bimaxillary (inferior malar) breadth          ZMB

Zygomaxillary subtense (subspinale            SSS
projection from bimaxillary breadth)

Bifrontal (upper facial) breadth              FMB

Nasion-frontal subtense (nasion               NAS
projection from binfrontal breadth)

Biorbital breadth (breadth from dacryon       EKB
to ectoconchion)

Dacryon subtense (dacryon projection          DKS
from biorbital breadth)

Interorbital breadth (across the dacrya)      DKB

Nasodacryal subtense (least projection        NDS
of nasal bones from interorbital

Simotic chord (least breadth across the       WNB
nasal bones)

Simotic subtense (projection of the           SIS
nasal bridge from simotic chord)

Inferior malar length (left)                  IML

Maximum malar length (left)                   XML

Malar subtense (greatest projection of        MLS
malar from maximum malar length)

Cheek height (left)                           WMH

Supraorbital projection (projection of        SOS
left superciliary ridge)

Glabella projection (greatest projection      GLS
from nasion-supraglabellare chord)

Foramen magnum (basion to opisthion)          FOL

Frontal (nasion to bregma) chord              FRC

Frontal subtense (greatest projection         FRS
from frontal chord)

Frontal fraction (distance from nasion        FRF
where greatest frontal projection falls)

Parietal (bregma to lambda) chord             PAC

Parietal subtense (greatest projection        PAS
from parietal chord)

Parietal fraction (distance from bregma       PAF
where greatest parietal projection

Occipital (lambda to opisthion) chord         OCC

Occipital subtense (greatest projection       OCS
from occipital chord)

Occipital fraction (distance from lambda      OCF
where greatest occipital projection

Cranial index (100 * XCB/GOL)               GOL:XCB

Vault length/height index (100 * BBH/       GOL:BBH

Frontal curvature index (100 * FRS/FRC)     FRC:FRS

Parietal curvature index (100 * PAS/PAC)    PAC:PAS

Occipital curvature index (100 * OCS/       OCC:OCS

Gnathic index (100 * BPL/BNL)               BNL:BPL

Posterior craniofacial index (100 * ASB/    ZYB:ASB

Transverse craniofacial index (100 *        XCB:ZYB

Upper facial index (100 * NPH/ZYB)          ZYB:NPH

Bizygomatic/bimaxillary index (100 *        ZYB:ZMB

Nasal index (100 * NLB/NLH)                 NLH:NLB

Orbital index (100 * OBH/OBB)               OBB:OBH

Frontal flatness index (100 * NAS/FMB)      FMB:NAS

Orbital flatness index (100 * DKS/EKB)      EKB:DKS

Maxillary flatness index (100 * SSS/ZMB)    ZMB:SSS

Nasodacryal index (100 * NDS/DKB)           DKB:NDS

Simotic index (100 * SIS/WNB)               WNB:SIS

TABLE 3: Relationship between measurements' mean and standard
deviation for Indian series.

Measurements        Untransformed variables  Variables transformed to
included                                        base 10 logarithms

                    Pearson's r   Slope of   Pearson's r   Slope of
                                  best-fit                 best-fit
                                    line                     line

All measurements,      0.743       0.033        0.839        0.429
all series

All measurements       0.812       0.034        0.861        0.424
except fractions,
all series

All measurements       0.887       0.032      0.907 (a)    0.411 (b)
except fractions,
all series with
[greater than or
equal to] 30
values per

(a) Pearson's r value is 0.908 for northern Indians and 0.907
for southern Indians.

(b) Slope of best-fit line is 0.408 for northern Indians and 0.413
for southern Indians.

TABLE 4: Relationship between measurements' mean and range for
Indian series.

Linear            Untransformed variables  Variables transformed to
measurements                                  base 10 logarithms
                  Pearson's r   Slope of   Pearson's r   Slope of
                                  best                     best
                                fit line                 fit line

All                  0.699       0.142        0.752        0.438
all series

All                  0.752       0.146        0.767        0.433
fractions, all

All                  0.852       0.166      0.878 (a)    0.420 (b)
fractions, all
series with
[greater than
or equal to] 30
values per

(a) Pears on's r value is 0.883 for northern Indians and 0.876
for southern Indians.

(b) Slope ofbest fit line is 0.431 for northern Indians and 0.411
for southern Indians.

TABLE 5: Relationship between indices' mean and variability
(other than nasal flatness indices) for Indian series.

Series included        Untransformed variables  Variables transformed to
and measure of                                     base 10 logarithms
compared               Pearson's r   Slope of   Pearson's r   Slope of
                                       best                   best fit
                                     fit line                   line

All series (standard      0.644       0.028        0.684        0.369

All series with           0.740       0.028      0.781 (a)    0.367 (b)
[greater than or
equal to] 30 values
per index (standard

All series (ranges)       0.509       0.116        0.493        0.351

All series with           0.637       0.126      0.684 (c)    0.327 (d)
[greater than or
equal to] 30 values
per index (ranges)

(a) Pearson's r value is 0.797 for northern Indians and 0.768 for
southern Indians.

(b) Slope of best fit line is 0.371 for northern Indians and
0.364 for southern Indians.

(c) Pearson's r value is 0.725 for northern Indians and 0.660 for
southern Indians.

(d) Slope of best fit line is 0.345 for northern Indians and
0.316 for southern Indians.

TABLE 6: Count of measurements for which males are statistically
significantly larger than females in Indian series.

                          Sexual dimorphism

Series      Universal (a)    Universal    Typical if
                              if well        well
                            sampled (b)   sampled (c)

Konkani           6              2             5
Urdu              6              2             3
Malayalam         6              7             1
Tulu              6             16             9
Telugu            6             16            12
Haryanavi         6             16             5
Punjabi           6             16             7
Kannada           6             16            10
Tamil             6             16            11
Hindi             6             16            12

                       Sexual dimorphism

Series      Weak (d)   Unclear (e)   Total for 47

Konkani        0            0        13/47 (27.7%)
Urdu           0            0        11/47 (23.4%)
Malayalam      0            0        14/47 (29.8%)
Tulu           1            0        32/47 (68.1%)
Telugu         3            0        37/47 (78.7%)
Haryanavi      1            0        28/47 (59.6%)
Punjabi        2            0        31/47 (66.0%)
Kannada        3            0        35/47 (74.5%)
Tamil          3            0        36/47 (76.6%)
Hindi          5            0        39/47 (83.0%)

(a) BBH, AUB, MDH, ZYB, JUB, and OBB (males significantly larger
in every series).

NLB, WCB, XML, and WMH (males significantly larger in every
series with at least 30 males).

and IML (males significantly larger in 5 to 6 series with at
least 30 males).

(d) PAS, PAF, OCC, OCS, NAS, DKB, DKS, and SSS (males
significantly larger in just 1 to 4 series with at least 30

(e) FRS, FOL, OBH, and WNB (males not significantly larger in any

TABLE 7: Significant craniometric differences between northern
and southern Indians.

Measurement   Males (in bold if      Females (in bold if
or index      northern Indians       northern Indians
              values higher)         values higher)

SOS           All comparisons        All comparisons
              except Haryanavis      except Hindis and
              cf. Konkanis and       Haryanavis cf.
              Telugu                 Konkanis and Urdu

OBB           All comparisons        Punjabis cf. Telugu;
                                     Haryanavis cf.
                                     Telugu and
                                     Malayalam; Hindis
                                     cf. Konkanis and

OBB:OBH       All comparisons        All comparisons
                                     involving Telugu,
                                     Tulu, Tamils and
                                     Malayalam, plus
                                     Punjabis and
                                     Haryanavis cf.

DKB           All comparisons        All comparisons
              involving Telugu,      involving Telugu,
              Konkanis, Tamils,      Tulu and Tamils,
              and Malayalam          plus Punjabis and
                                     Haryanavis cf.
                                     Kannada, and
                                     Haryanavis cf.

DKS           All comparisons        All comparisons
              except Haryanavis      involving Hindis,
              cf. Telugu and         plus Haryanavis cf.
              Malayalam              Malayalam

SSS           All comparisons        All comparisons
              involving Tulu and     involving
              Kannada, plus          Haryanavis, plus
              Haryanavis cf.         Punjabis cf. Tulu
              Tamils and Malayalam   and Malayalam, and
                                     Hindis cf. Tulu

BBH:GOL       All comparisons        Punjabis cf. Kannada
              involving Hindis and   and Malayalam;
              Haryanavis, plus       Haryanavis cf.
              Punjabis cf.           Konkanis, Kannada,
              Konkanis               Tulu and Tamils;
                                     Hindis cf. Konkanis,
                                     Kannada and Tamils

STB           All comparisons        No comparisons
              involving Punjabis
              and Hindis, plus
              Haryanavis cf. Tulu

XFB           All comparisons        Hindis cf. Telugu,
              involving Hindis,      Kannada and Tamils
              plus Punjabis and
              Haryanavis cf.
              Kannada, Tulu and
              Tamils, and Punjabis
              cf. Konkanis and

TABLE 8: Comparisons between Indians and Worldwide Series on
Average Indices (Males) (a).

Index            Indians'     Similarities

Cranial           Narrow      Africa, Eskimos,
                              Southwest Pacific,

Vault length-      Wide       Polynesia/
height                        Micronesia, Veddas

Frontal           Narrow      Ainu

Parietal           Wide       Egypt, Europe,
curvature                     Veddas

Occipital          Wide       Ainu, East Asia

Gnathic          Moderate     Egypt, Europe,
                              Micronesia, Veddas

Posterior         Narrow      Europe

Transverse        Narrow      Africa, Ainu

Upper facial     Moderate     Amerindians, East
                              Asia, Europe

Bizygomatic-     Moderate     Bushmen, Veddas

Nasal             Narrow      East Asia,

Orbital            Wide       Ainu, Bushmen,
                              Europe, Southwest

Frontal            Wide       None

Orbital            Wide       None (Vedda
flatness                      comparison

Maxillary        Moderate     Andamans, Egypt,
flatness                      Veddas

Nasodacryal        Wide       Amerindians, Egypt,
                              Eskimos, Europe
                              (Vedda comparison

Simotic            Wide       Amerindians, Europe

Index           Dissimilarities

Cranial         Amerindians,
                Andamans, Buriats,
                East Asia, and

Vault length-   Bushmen

Frontal         Amerindians,
curvature       Bushmen, Polynesia/
                Micronesia, and
                Southwest Pacific

Parietal        Bushmen

Occipital       Buriats, Bushmen

Gnathic         Africa, Southwest

Posterior       Africa, Amerindians,
craniofacial    Buriats, Bushmen,
                Eskimos, Polynesia/
                Micronesia, and
                Southwest Pacific

Transverse      Andamans, Buriats,
craniofacial    Bushmen, Egypt,
                Eskimos, and Europe

Upper facial    Bushmen, Egypt,
                Southwest Pacific,
                and Veddas

Bizygomatic-    Ainu, Europe,
bimaxillary     Southwest Pacific,
                and Polynesia/

Nasal           Africa, Bushmen,
                Egypt, Eskimos,
                Europe, and
                Southwest Pacific

Orbital         Amerindians,
                Andamans, and

Frontal         All except Veddas
flatness        and Egypt

Orbital         All except Veddas
flatness        (comparison

Maxillary       Ainu, Buriats,
flatness        Bushmen, East Asia,
                and Eskimos

Nasodacryal     Africa, Buriats,
                Bushmen, and East

Simotic         Africa, Andamans,
                Buriats, Bushmen,
                East Asia, and
                Southwest Pacific

(a) "Africa" and "East Asia" in this table respectively correspond
to "other Africa" and "other East Asia" in Figures 3-5.

TABLE 9: Variability (per cent) explained by the first five
principal components.

Sex       PC1    PC2   PC3   PC4   PC5

Males     30.1   8.0   6.8   5.1   4.8
Females   275    8.3   7.7   5.4   5.2

TABLE 10: Factor loadings of the measurements (a) on the first
three principal components.

Measurement   PC 1 Males   PC 2 Males   PC 3 Males   PC 1 Females

NAS             -0.126       0.766        0.030         -0.079
DKS             -0.391       0.652        0.123         -0.275
SIS             -0.134       0.588        0.218         -0.084
NDS             0.092        0.483        0.023         0.171
GOL             0.691        0.485        -0.107        0.650
NOL             0.697        0.468        -0.073        0.677
WNB             -0.020       0.427        0.063         -0.033
SSS             0.126        0.431        -0.189        0.084
BNL             0.627        0.429        -0.148        0.642
FRC             0.509        0.328        0.317         0.493
PAC             0.364        0.301        -0.080        0.271
OBB             0.437        0.323        0.061         0.509
FRS             -0.085       0.184        0.366         -0.044
BBH             0.523        0.205        0.135         0.462
STB             0.232        0.040        0.770         0.294
OCS             0.359        0.191        -0.055        0.301
XFB             0.392        -0.031       0.765         0.424
NLH             0.659        0.044        0.251         0.671
PAS             0.044        0.057        0.023         0.027
BPL             0.626        0.177        -0.473        0.618
FMB             0.762        0.112        -0.118        0.744
MDH             0.442        0.051        -0.007        0.393
OCC             0.493        0.108        0.085         0.426
NPH             0.671        0.008        0.199         0.678
GLS             0.356        0.085        -0.295        0.302
EKB             0.818        0.009        -0.140        0.800
FOL             0.427        -0.019       0.096         0.379
OBH             0.469        -0.059       0.219         0.467
DKB             0.467        -0.060       -0.184        0.348
ASB             0.654        -0.066       0.248         0.607
NLB             0.478        -0.032       -0.254        0.449
IML             0.575        0.005        -0.386        0.545
MDB             0.569        -0.029       -0.104        0.542
XML             0.713        -0.030       -0.218        0.681
MAB             0.705        -0.122       -0.098        0.640
ZMB             0.697        -0.158       -0.088        0.642
WMH             0.630        -0.140       0.092         0.609
JUB             0.892        -0.180       -0.086        0.860
AUB             0.803        -0.191       0.281         0.785
ZYB             0.892        -0.183       0.037         0.869
SOS             0.508        -0.124       -0.304        0.456
WCB             0.583        -0.287       0.280         0.587
XCB             0.687        -0.307       0.424         0.667
MLS             0.517        -0.296       -0.271        0.472

Measurement   PC 2 Females   PC 3 Females

NAS              0.769          -0.212
DKS              0.716          -0.101
SIS              0.642          0.127
NDS              0.473          -0.071
GOL              0.411          -0.259
NOL              0.400          -0.214
WNB              0.414          -0.119
SSS              0.351          -0.272
BNL              0.346          -0.226
FRC              0.399          0.202
PAC              0.313          -0.282
OBB              0.257          -0.093
FRS              0.345          0.217
BBH              0.273          0.049
STB              0.269          0.664
OCS              0.050          -0.002
XFB              0.235          0.670
NLH              0.137          0.293
PAS              0.122          -0.160
BPL              -0.043         -0.524
FMB              0.072          -0.263
MDH              0.084          0.097
OCC              0.004          0.134
NPH              0.082          0.236
GLS              -0.094         -0.341
EKB              -0.042         -0.244
FOL              0.084          0.128
OBH              0.004          0.269
DKB              -0.030         -0.280
ASB              -0.006         0.306
NLB              -0.117         -0.299
IML              -0.173         -0.423
MDB              -0.144         -0.030
XML              -0.161         -0.209
MAB              -0.146         -0.059
ZMB              -0.114         -0.026
WMH              -0.193         0.218
JUB              -0.153         -0.096
AUB              -0.118         0.346
ZYB              -0.160         0.099
SOS              -0.219         -0.324
WCB              -0.101         0.370
XCB              -0.194         0.449
MLS              -0.351         -0.219

(a) Measurements in approximate order (weighted across males and
females) from a large positive loading to a large negative
loading on the second principal component.

TABLE 11: Mahalanobis-D distances after seriation, Mosimann indices,
males, Indians, and Kalahari Bushmen compared with each other and
with other series.

Series                 Punjabis   Hindis   Haryanavis   Telugu

Buriats                  7054     7.079      7.389      7.791
Eskimos                 6.232     6.148      6.717      6.655
Australians             5.615     5.685      5.939      6.433
Tasmanians              6.077     6.169      6.345      6.664
New Britain Tolai       5.758     5.770      6.299      6.320
Moriori                 6.411     6.377      6.292      7.240
Easter Island           5.573     5.477      5.460      6.186
Hawaiians               5.563     5.650      5.850      6.210
Guam                    5.157     5.207      5.625      5.868
Anyang Chinese          5.886     5.999      6.498      6.723
Hainan Chinese          5.067     5.220      5.832      6.270
Philippines             4.935     5.092      5.718      6.053
North Japan             5.157     5.414      6.059      6.602
South Japan             4.743     4.911      5.566      5.957
Taiwan Atayal           4.912     5.024      5.702      6.083
Ainu                    4.547     4.679      5.044      5.401
Arikara (America)       5.438     5.273      5.550      6.272
Santa Cruz (America)    5.166     5.241      5.630      6.500
Peru (America)          4.871     4.802      5.038      6.013
Zalavar (Europe)        3.795     3.648      4.061      4.693
Norse (Europe)          4.425     4.278      4.067      5.317
Egypt                   4.214     4.257      4.201      5.125
Berg (Europe)           4.986     4.983      5.049      5.829
Andaman Islanders       5.125     5.801      6.275      6.417
Zulu (Africa)           4.555     4.971      5.494      5.948
Dogon (Africa)          5.745     6.290      6.580      6.901
Teita (Africa)          5.084     5.216      5.381      6.240
Punjabis                          1.615      2.624      3.353
Hindis                                       1.972      2.867
Haryanavis                                              3.583

Series                 Tamils   Kannada   Bushmen

Buriats                8.067     8.099     7.154
Eskimos                7.037     7.340     7.815
Australians            6.821     7.153     6.603
Tasmanians             7.070     7.637     6.440
New Britain Tolai      6.753     7.178     7.162
Moriori                7.717     7.819     8.128
Easter Island          6.798     7.019     7.381
Hawaiians              6.743     6.746     7.363
Guam                   6.511     6.589     7.246
Anyang Chinese         7.214     7.035     6.748
Hainan Chinese         6.716     6.537     6.761
Philippines            6.501     6.466     6.226
North Japan            7.067     6.906     6.449
South Japan            6.440     6.349     6.438
Taiwan Atayal          6.651     6.682     6.134
Ainu                   6.065     6.217     5.912
Arikara (America)      6.610     6.882     7.799
Santa Cruz (America)   6.754     7.015     6.906
Peru (America)         6.371     6.501     7.224
Zalavar (Europe)       5.349     5.766     6.022
Norse (Europe)         5.943     6.227     6.210
Egypt                  5.628     5.836     6.619
Berg (Europe)          6.335     6.619     6.208
Andaman Islanders      6.993     6.908     6.153
Zulu (Africa)          6.399     6.421     5.060
Dogon (Africa)         7.420     7.216     5.670
Teita (Africa)         6.479     6.421     5.366
Punjabis               3.618     3.392     6.631
Hindis                 3.074     3.397     7.053
Haryanavis             3.594     3.819     7.234
Telugu                 2.190     3.484     7.520
Tamils                           2.430     7.705
Kannada                                    7.785

TABLE 12: Mahalanobis D-distances after seriation, Mossiman indices,
females, and Indians compared with each other and with other series.

Series                 Hindis   Punjabis   Haryanavis

Buriats                7.768     7.937       8.365
Eskimos                6.452     6.710       7.128
Easter Island          6.477     6.587       6.456
Tasmanians             6.601     6.706       6.836
Australians            5.687     5.959       6.270
New Britain Tolai      6.031     6.330       6.579
Kalahari Bushmen       6.730     6.968       7.607
Teita (Africa)         6.170     6.558       6.765
Dogon (Africa)         6.230     6.410       6.975
Zulu (Africa)          5.244     5.607       6.089
Andaman Islanders      6.572     6.513       7.319
Mokapu Hawaiians       6.471     6.374       6.744
Moriori                6.387     6.329       6.179
Guam                   5.632     5.678       6.075
Hainan Chinese         5.697     5.820       6.410
North Japan            5.741     5.634       6.484
South Japan            5.578     5.694       6.341
Taiwan Atayal          5.244     5.268       6.022
Ainu                   5.024     5.117       5.759
Santa Cruz (America)   6.135     6.300       6.685
Arikara (America)      5.415     5.732       5.716
Peru (America)         5.656     6.027       6.038
Norse (Europe)         4.625     4.944       4.855
Zalavar (Europe)       4.482     4.785       5.115
Egypt                  4.823     5.039       5.217
Berg (Europe)          5.352     5.655       5.796
Hindis                           1.887       2.238
Punjabis                                     2.743

Series                 Telugu   Tamils   Kannada

Buriats                8.071    8.842     8.558
Eskimos                6.650    7.298     7.452
Easter Island          7.204    7.591     7.758
Tasmanians             6.892    7.683     8.139
Australians            6.299    7.163     7.195
New Britain Tolai      6.822    7.497     7.363
Kalahari Bushmen       7.225    7.971     7.968
Teita (Africa)         6.922    7.419     7.231
Dogon (Africa)         6.882    7.530     7.411
Zulu (Africa)          6.258    6.814     6.751
Andaman Islanders      6.833    7.660     7.539
Mokapu Hawaiians       6.718    7.359     7.269
Moriori                6.678    7.502     7.394
Guam                   6.181    6.909     6.802
Hainan Chinese         6.360    6.941     6.869
North Japan            6.469    7.301     7.147
South Japan            6.268    7.082     6.981
Taiwan Atayal          5.987    6.686     6.758
Ainu                   5.367    6.293     6.432
Santa Cruz (America)   6.785    7.765     7.603
Arikara (America)      5.933    6.809     6.510
Peru (America)         6.394    7.244     6.882
Norse (Europe)         5.177    6.175     6.127
Zalavar (Europe)       5.225    6.192     6.111
Egypt                  5.339    6.314     6.211
Berg (Europe)          6.049    6.958     6.826
Hindis                 2.892    3.196     3.123
Punjabis               2.725    3.207     3.137
Haryanavis             3.620    3.862     3.681
Telugu                          2.099     3.134
Tamils                                    2.751
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Title Annotation:Research Article
Author:Raghavan, Pathmanathan; Bulbeck, David; Pathmanathan, Gayathiri; Rathee, Suresh Kanta
Publication:International Journal of Evolutionary Biology
Article Type:Report
Date:Jan 1, 2014
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