Increasing the sensitivity of advanced stage of prostate cancer to chemotherapeutic drugs-induced apoptosis by targeting miR-205 and miR-31.
METHODOLOGY: We used microRNA expression analysis to find the microRNAs which are differentially expressed in the two cell lines and the Apoptotic assay to determine their response to various chemotherapeutic drugs. We also did Western blot analysis to see the difference in the protein expression of anti-apoptotic genes in these cell lines.
RESULTS: We have found that WPE-1 NB26 cells express less miR-205 and miR-31 than WPE-1 NA22 cells, and hence have higher levels of anti-apoptotic proteins like Bcl-w and E2F6, respectively. This makes them resistant to the treatment with various apoptotic agents. Also, the Bcl-w and E2F6 stable expressing WPE-1 NA22 cell lines showed high resistance to various chemotherapeutic drugs.
CONCLUSIONS: miR-205 and miR-31 play an important role in apoptosis induced by chemotherapeutic drugs and can be a useful strategy to treat the prostate cancer at an advanced stage.
Namrata Bhatnagar *, Xia Li and Bin Guo
Department of Pharmaceutical Sciences, North Dakota State University, Fargo, ND-58105
(1.) Scardino P.T., et al, 1992, Human Pathology, 23, 211-222.
(2.) Webber M.M., et al, 2001, The Prostate, 47:1-13.
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|Author:||Bhatnagar, Namrata; Li, Xia; Guo, Bin|
|Publication:||Proceedings of the North Dakota Academy of Science|
|Date:||Apr 1, 2009|
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