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In vitro culture and antibacterial activity of Lantana.

Lantana (Lantana camera) is considered an invasive plant which grows in disturbed areas. It also is a medicinal plant used to treat a number of aliments including skin irritation, as a sedative, and to treat coughs, bronchitis, rheumatism and stomach problems. The objective of this project was to establish an in vitro culture protocol and to determine the antibacterial activity of Lantana extracts. Explants used were leaf sections and apical meristems, which were disinfected in 30% Clorox for 15 to 20 minutes followed by rinses in sterile distilled water. Explants were cultured on: MS salts with sucrose, inositol, NAA, kinetin and thiamine HCl; MS Minimal Organics with pyridoxine, nicotinic acid, sucrose, IAA and BA; or MS Medium with inositol, NAA and kinetin. Pure cultures of Echeria coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus epidermidis, and Staphylococcus aureus were tested with aqueous extracts of leaf tissue. Leaf explants formed callus on all media tested; meristems developed and the explant turned green by 21 days incubation. Growth of Echeria coli and Pseudomonas aeruginosa was not inhibited by the plant extracts whereas the growth of Klebsiella pneumoniae, Streptococcus pyogenes, Staphylococcus epidermidis, and Staphylococcus aureus were. Reported effectiveness of Lantana on those organisms causing respiratory infections was confirmed in this study.
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Author:Camper, N.D.; Clark, Kimberly
Publication:Bulletin of the South Carolina Academy of Science
Date:Jan 1, 2005
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