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Immunolocalization of FAK in NGF-treated PC12 cells. (Molecular Biology and Genetics 02:00 PM, Saturday, April 5, 2003 Brewer/Frost Science 141 Dr. Beth Berger Pritts-Presiding).

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Focal Adhesion Kinase (FAK), a 125 kDa non-receptor tyrosine kinase, is known to be Important in intracellular signaling cascades and cell adhesion. Although FAK has been studied in short-term focal adhesion turnover, research into FAK's role in adhesion during long-term neurogenesis has been limited. PC12 cells, a rat pheochromocytoma cell line, provide an ideal system to study FAK, focal adhesions and neurogenesis. PC12 cells can be induced to differentiate into a neuron-like cell and project neurite extensions from the cell body within 24 hours of stimulation with Nerve Growth Factor (NGF). Previous studies from this lab indicate that the levels of FAK protein in PC12 cells al(er five day stimulation with NGF did not change; however, the amount of activated (phosphorylated) FAK within the cells did increase (Smith and Selickman, unpublished data). In this study, location of both FAK and activated FAK within PC12 cells al(er a five day stimulation with NGF will be examined by immunocytochemistry and confocal microscopy. Because activated FAK is important in cell adhesion, we hope to observe active FAK located within the neurite extensions, primarily in the growth cones, where it would be important in regulating neurite adhesion and projection.

SARAH E. MEYERS MEYERS_S@DENISON.EDU (DR. CATHERINE N. SMITH SMITHC@DENISON.EDU), DENISON UNIVERSITY, SLAYTER BOX 1717, GRANVILLE OH 43023
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Author:Meyers, Sarah E.; Smith, Catherine N.
Publication:The Ohio Journal of Science
Article Type:Abstract
Date:Mar 1, 2003
Words:221
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