Ilheus virus infection in human, Bolivia.
In November 2005, a 15-year-old boy (farmer) sought medical attention in a health clinic in Magdalena, Bolivia, after having fever for 5 days. The patient's symptoms included malaise, asthenia, conjunctival injection, vesicular rash, facial edema, arthralgia, myalgias, bone pain, abdominal pain, headache, and earache. Signs of cardiac, neurologic, or renal damage were not detected. A blood specimen was obtained during the clinic visit, and a convalescent-phase sample was obtained 24 days after onset of symptoms. At that follow-up visit, the patient reported a full recovery from his symptoms. Both samples were sent to the Naval Medical Research Unit No. 6 in Lima, Peru, for processing as part of a clinic-based study to determine the etiology of febrile illnesses in Bolivia (7). The study was approved by the Naval Medical Research Unit No. 6 Institutional Review Board (Navy Medical Research Center Detachment 2000.0008) and conducted in collaboration with the Bolivia Ministry of Health.
Serologic analyses showed a 64-fold IgM seroconversion between the acute-phase (<100) and convalescent-phase samples (6,400) by using an IgM ELISA as described (8). Samples were also tested by ELISA for the following arboviruses: West Nile virus, dengue virus, Oropouche virus, Guaroa virus, Rocio virus, St. Louis encephalitis virus, yellow fever virus, Venezuelan equine encephalitis virus, and Mayaro virus. All test results were negative for these viruses. Virus isolation was attempted on the acute-phase serum sample by using Vero and C6/36 cells, but the culture did not yield any virus. Attempts to isolate virus by intracranial inoculation in suckling mice were also unsuccessful (University of Texas Medical Branch, Institutional Animal Care and Use Committee protocol 9505045).
Viral RNA was extracted from the acute-phase sample and reverse transcription PCR specific for a portion of the nonstructural protein 5 gene was performed by using a described method (9). A 189-bp PCR product was obtained, purified, and sequenced by using flavivirus primers FU1 and cFD2 (9) and further analyzed by using BLAST (www.ncbi. nlm.nih.gov/blast), resulting in -95% homology to ILHV. Phylogenetic analysis with neighbor-joining and parsimony methods grouped the nucleotide sequence of the ILHV virus from Bolivia with ILHV strains from Ecuador and Peru (Figure).
Magdalena is a tropical city in northern Bolivia that borders Brazil. The city is surrounded by rivers and chestnut fields, and agriculture and fishing are the main sources of employment. Despite having ecoepidemiologic conditions similar to those in other locations with a history of ILHV transmission, the virus had not been detected in the area. The patient had no travel history in the 30 days preceding his illness, indicating that the virus is probably endemic to the area.
Mild unspecific symptoms, a short viremic period, and lack of advanced confirmatory laboratory techniques in situ are some of the barriers impeding the diagnosis of ILHV in disease-endemic areas. High levels of antibody cross-reactivity among flaviviruses, which are also endemic to the area, might render diagnosis even more difficult. The presence of the main ILHV vector, Psorophora sp. mosquitoes, in the city suggests that much of the population that labors outdoors may be at risk for ILHV infection.
We thank Pedro Palermo and Vidal Felices for laboratory technical support.
This study was supported by the US Department of Defense Global Emerging Infections Systems Research Program, Work Unit No. 800000.82000.25GB. B0016.
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Erika A. Venegas, Patricia V. Aguilar,  Cristhopher Cruz, Carolina Guevara, Tadeusz J. Kochel, Jorge Vargas, and Eric S. Halsey
Author affiliations: Naval Medical Research Unit 6, Lima, Peru (E.A. Venegas, P.V. Aguilar, C. Cruz, C. Guevara, T.J. Kochel, E.S. Halsey); New York University, New York, New York, USA (E.A. Venegas); and Centro Nacional de Enfermedades Tropicales, Santa Cruz, Bolivia (J. Vargas)
Address for correspondence: Eric S. Halsey, Naval Medical Research Unit 6, 3230 Lima Pl, Washington, DC 20521-3230, USA; email: eric. firstname.lastname@example.org
 Current affiliation: University of Texas Medical Branch, Galveston, Texas, USA.
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|Author:||Venegas, Erika A.; Aguilar, Patricia V.; Cruz, Cristhopher; Guevara, Carolina; Kochel, Tadeusz J.; V|
|Publication:||Emerging Infectious Diseases|
|Date:||Mar 1, 2012|
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