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Host cell capture-quantitative sequence detection of potentially infectious viruses.


Host cell capture-quantitative sequence detection of potentially infectious viruses.

Ed. by George D. Di Giovanni et al.

American Water Works Assn.


48 pages




Enteric viruses are routinely detected in raw sewage, surface waters, and groundwater, and have the greatest infectivity among waterborne pathogens and outbreaks of illness due to contaminated water. This report describes research on a new method, referred to as host cell capture quantitative sequence detection (HCC-QSD), for detecting infectious waterborne viruses, and its potential for integration with current virus concentration, cell culture, and PCR-based detection strategies. Material is in sections on evaluation of different host cell lines for the capture of enteric viruses and optimization of capture conditions, HCC-QSD of viruses and evaluation of standard and advanced nucleic acid purification methods, and evaluation of HCC-QSD to distinguish potentially infectious viruses from chlorine- and UV-inactivated viruses. Giovanni is affiliated with Texas AgriLife Research, Texas A&M University.

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Publication:SciTech Book News
Article Type:Book review
Date:Jun 1, 2009
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