Printer Friendly

Histological study of the prostate and vesicular glands in Eastern European hedgehog (Erinaceous concolor).

Introduction

The male accessory sex glands of mammals include the prostate gland, the vesicular glands, the glands of the ampulla and the bulbourethral glands. These glands depend on androgen and play an important role in the reproductive function [1,2]. Macroscopic and microscopic anatomy of these glands varies widely among different species of mammalian vertebrates [3]. Histology of Prostate and vesicular glands have studied in some mammals with almost different findings [1]. Hedgehogs are minute mammals of Erinaceidae family. These exotic pets are nocturnal and their body is coated with spiny like needles. Recently they are receiving a great attention among pet owners. These animals have become increasingly popular as an exotic household pet due to being unique, cute, and low and ways maintenance pets [4]. The aim of the present study was to histological evaluate the prostate and vesicular gland in Eastern European hedgehog (Erinaceous concolor).

Materials and Methods

This investigation was performed on 5 healthy and adult male hedgehogs. Animals were captured from suburbs of Tabriz city of East Azerbaijan, Iran and euthanized by intra-peritoneal injection of sodium pentobarbital (250 mg/kg). The research protocol was approved by the ethical committee of the University of Tabriz. The prostate and vesicular glands were dissected out and tissue samples fixed by immersion in %10 formalin. Then samples dehydrated in graded ethanol, cleared in xylen, impregnated and embedded in paraffin wax. Sections of 6-7[micro] thick were obtained with Leitz microtome model 1512 and stained with hematoxylin/eosin (H & E) [5]. All the slides were examined and photographed with an Olympus BH-2 light microscope to evaluate the histological features.

Results

The findings showed that the wall of prostate gland was consisted of a connective tissue capsule. From the capsule, many trabeculae divided the gland into fluid filled lobes (Figure 1). The mucosa (epithelium and lamina propria) was thrown into branching folds. The epithelium of the prostate was simple columnar supported by basement membrane (Figures 2 and 3). Almost round nuclei and prominent central nucleolus were observed in epithelium. The vesicular gland was observed to be surrounded by connective tissue capsule with trabeculae originating from it (Figure 4). The gland was lined with simple columnar cells of varying heights with oval Nuclei (Figure 5). These secretory epithelium had surface blebs (large flaccid vesicles) giving a ragged appearance to the cells (Figure 6).

Discussion

Accessory sex glands are important in reproductive functions of mammals. Some researchers have showed that secretions of accessory sex glands influence sperm physiology and fertilization. From another prospective, secretions of the accessory sex glands are able to enhance the fertilizing capacity of some domestic animals sperm [6,7]. So, the histological and physiological study of these glands can help in understanding of reproductive biology of mammals. It has been revealed that in the stallion and dog, the epithelium of the prostate gland is consisted of tall columnar secretory cells that are similar to findings of the present study.

Adebayo et al. [1] have reported that in greater cane rat (Thryonomys swinderianus, Temminck) the prostate is surrounded by connective tissue capsule and compartments are lined with simple columnar cells containing nuclei with eccentrically located nucleoluses. In Caspian miniature horse, the secretory epithelium of prostate is cuboidal to columnar and/or pseudostratified [8].

Vesicular glands of Eastern European hedgehog is almost similar to other mammals is having simple columnar epithelium [1]. In carnivores these glands are absent but are true vesicles in the stallion. In bull the secretory epithelium of vesicular glands is tall columnar with apical blebs that is similar with the results of present study [2].

doi: 10.4172/0974-8369.1000205

References

[1.] Adebayo AO, Oke BO, Akinloye AK (2009) The gross morphometry and histology of the male accessory glands in the Greater Cane rat (Thryonomys swinderianus, Temminck). Journal of Veterinary Anatomy 2: 41-51.

[2.] Aughey E, Frye FL (2001) Comparative Veterinary Histology. Manson Publishing 174-175.

[3.] Thomson AA1, Marker PC (2006) Branching morphogenesis in the prostate gland and seminal vesicles. Differentiation 74: 382-392.

[4.] Youssefi MR, Rahimi MT, Halajan A, Moosapour AA, Nikzad R, et al. (2013) Helminth parasites of Eastern European hedgehog (Erinaceous concolor) in Northern. Iran. Iranian Journal of Parasitology 8: 645-650.

[5.] Bancroft JD, Stevens A (1977) Theory and Practice of Histological Techniques. Churchill Livingstone, 10-45.

[6.] Henault MA1, Killian GJ, Kavanaugh JF, Griel LC Jr (1995) Effect of accessory sex gland fluid from bulls of differing fertilities on the ability of cauda epididymal sperm to penetrate zona-free bovine oocytes. Biol Reprod 52: 390-397.

[7.] Moura AA1, Chapman DA, Koc H, Killian GJ (2007) A comprehensive proteomic analysis of the accessory sex gland fluid from mature Holstein bulls. Anim Reprod Sci 98: 169-188.

[8.] Sodagar Amiri A, Rezaian M, Gilanpour H, Adib Moradi M, Rostami M, et al. (2010) Anatomical and histological study of accessory sex glands in Caspian miniature horse. Veterinary Journal of Islamic Azad University 7: 11-16.

Banan Khojasteh SM (1) *, Rodbari. F (1), Halimi Tabrizi. F (1) and Delashoub. M (2)

(1) Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

(2) Department of Basic Sciences, Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran

* Corresponding author: Banan Khojasteh SM, Associate Professor of Animal Biology, Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran, Tel: +984113392728; Fax: +984113356027; E-mail: smbanan@tabrizu.ac.ir

Received date: April 9, 2014, Accepted date: June 28, 2014, Published date: July 5, 2014
COPYRIGHT 2014 HATASO Enterprises, LLC
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2014 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:Research Article
Author:Banan Khojasteh, S.M.; Rodbari, F.; Halimi Tabrizi, F.; Delashoub, M.
Publication:Biology and Medicine
Article Type:Report
Geographic Code:7IRAN
Date:Apr 1, 2014
Words:906
Previous Article:Impacted maxillary central incisors associated with bilateral mesiodentes: a case report.
Next Article:Two faces of Cathepsin D: physiological guardian angel and pathological demon.
Topics:

Terms of use | Privacy policy | Copyright © 2019 Farlex, Inc. | Feedback | For webmasters