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Hermaphroditic freshwater clams in the genus Corbicula produce non-reductional spermatozoa with somatic DNA content.

Introduction

The freshwater clam Corbicula leana has a special mode of reproduction: it is hermaphroditic and broods its larvae in the inner demibranchs (Miyazaki, 1936; Ikematsu and Yamane, 1977). A recent study of the chromosomes of C. leana showed that it has 54 somatic chromosomes, which, judging from its karyotype, are triploids (Okamoto and Arimoto, 1986). Okamoto and Arimoto (1986) suggested the possibility that C. leana reproduces by gynogenesis, which would account for the odd chromosome number: i.e., gynogenetic eggs are usually meiotically unreduced, and spermatozoa activate the development of eggs, but the paternal genome does not contribute to the offspring. Analysis of allozyme variation in C. leana also revealed that the species has much lower genetic variability than C. japonica and C. sandai (Sakai et al., 1994). In this study, we compare chromosome numbers and DNA content of somatic cells and spermatozoa in the hermaphroditic Corbicula species to test the gynogenesis hypothesis proposed by Okamoto and Arimoto (1986).

Materials and Methods

The Corbicula species were identified according to Habe (1977). C. leana and C. aff. fluminea were collected in Japan from an irrigation canal in Meiwa, Mie Prefecture, and from the Tade River, Saga Prefecture, respectively. In this study we use the name C. aff. fluminea for clams collected from Saga Prefecture because the morphology of the lateral teeth in these specimens was very similar to that of C. fluminea (Komaru et al., 1998). C. fluminea was also obtained from a cultured population in Shin Wu, Taiwan. For comparison using microfluorometry, specimens of C. sandai from Lake Biwa, Shiga Prefecture, were obtained at the market in Ootsu city.

Chromosomes

The clams were treated with 0.002% colchicine for 4-5 h. Gills and gonads were dissected out with scissors, treated with hypotonic solution (8 mM KCl), and fixed with Carnoy's fixative according to the procedure of Okamoto and Arimoto (1986). Cells were isolated from either gill or gonads, placed on slides, and stained with 2% Giemsa in phosphate buffer (pH 7.2). At least 4 clams were examined for each case (i.e., each combination of species and tissue type).

DNA microfluorometry

To compare the relative DNA content of spermatozoa and somatic tissue, cells were isolated on a glass slide by cutting a small piece of gonad or mantle in distilled water with a scalpel and air-drying it before fixing it with 70% ethanol. Spermatozoa and somatic cells from one individual were placed on the same slide. The cells were stained with the DNA-specific dye DAPI, and the relative DNA content (fluorescence intensity) per cell was estimated by microfluorometry as in Komaru et al. (1988). Spermatozoa could be easily distinguished from other spermatogenic cells because of their elongate and curved morphology.

Results

Mitotic and meiotic chromosomes

In four specimens of C. leana, we counted 54 chromosomes in the mitotic metaphase plates of somatic tissue [ILLUSTRATION FOR FIGURE 1A OMITTED] and about 18 chromosomes in the gonad [ILLUSTRATION FOR FIGURE 1B OMITTED]. In C. aft. fluminea, we observed 36 chromosomes in the mitotic metaphase in 16 cells, but the haploid number of 18 in 8 gonadal cells. Pachytene [ILLUSTRATION FOR FIGURE 1D OMITTED], diakinesis or metaphase I [ILLUSTRATION FOR FIGURE 1E OMITTED] figures were observed in the meiotic cells.

Relative DNA content of spermatozoa and somatic cells

In C. leana, C. aff. fluminea, and C. fluminea, the relative content of DNA in the spermatozoa was also identical to that in the somatic cells ([ILLUSTRATION FOR FIGURE 2 OMITTED]. and Table I). In C. sandai, however, the DNA content of the spermatozoa was half that of the somatic cells.

Discussion

Okamoto and Arimoto (1986) reported chromosome numbers of 3n = 54 for C. leana, 2n = 38 for C. japonica, [TABULAR DATA FOR TABLE I OMITTED] and 2n = 36 for C. sandai. In the present study, the chromosome number of C. aff. fluminea was determined to be 2n = 36 in gill tissue and 18 bivalents in gonads, indicating that C. fluminea is a diploid species. In contrast, C. leana has a somatic chromosome number of 54, but in the gonads, three homologous chromosomes form 18 trivalents. The synapsis of three homologous chromosomes should be incomplete. These results suggest that C. leana is a triploid species.

The microfluorometric measurements of DNA revealed that the hermaphroditic species C. leana and C. fluminea produced non-reductional spermatozoa; i.e., sperm and somatic cells had the same DNA content. On the other hand, the dioecious C. sandai produced reductional spermatozoa; the DNA content of sperm was half that of somatic cells. These results suggest that the cytokinesis of first or second meiosis in the spermatocytes of both C. leana and C. fluminea is abortive, and only one equal division occurs. Consequently, meiosis during spermatogenesis may be non-reductional, resulting in spermatozoa with somatic ploidy levels.

It is possible that triploid C. leana and diploid C. fluminea reproduce by gynogenesis, as O'Foighil and Thiriot-Quievreux (1991) observed in the bivalve Lasaea. In gynogenetic development, spermatozoa stimulate the development of eggs but do not contribute paternal genome to the offspring. In this case the somatic chromosome number may be restored to the eggs by premeiotic endomitosis or abortive meiosis (Cuellar, 1987). How did the triploid C. leana evolve from a diploid ancestral species? We assume that the diploid gynogenetic C. fluminea arose first. Later, the triploid C. leana may have evolved from the diploid gynogen by fusion of diploid and haploid gametes. The processes of meiosis and fertilization of eggs should be observed in C. leana and C. fluminea to understand the reproductive processes in these species with non-reductional spermatozoa.

Species of Corbicula show polymorphic shell color and morphology (Kuroda, 1938; Morton, 1986). The taxonomy of the Corbiculidae is in disarray as a result of intraspecific variation in shell morphology and insufficient biological information. Corbicula taxonomy cannot be clarified on the basis of shell morphology alone; as Morton (1986) suggested, ecological, genetic, and physiological studies will also be necessary. If the genus Corbicula includes polyploid species that reproduce by gynogenesis, as our results indicate, it will be difficult to apply the biological species concept to these clams (Mayr and Ashlock, 1991). The definition of species in Corbicula should be addressed after sufficient data on reproductive biology have been collected.

Acknowledgments

We especially thank Dr. W. L. Wu of the Institute of Zoology, Academica Sinica, Taipei, Taiwan, R.O.C., for providing valuable materials. We also thank Mr. T. Kawagishi of Mie University for technical assistance. This work was supported by a grant from the Science Technology Agency, Japan.

Literature Cited

Cuellar, O. 1987. The evolution of parthenogenesis: a historical perspective. Pp. 43-104 in Meiosis, P. B. Moens, ed. Academic Press, Orlando, FL.

Habe, T. 1977. Systematics of Mollusca in Japan - Bivalvia and Scaphopoda. Hokuryukan, 372 pp. (In Japanese.)

Ikematsu, W., and S. Yamane. 1977. Ecological studies of Corbicula leana PRIME-III. Bull. Jpn. Soc. Fish. 43: 1139-1146.

Komaru, A., Y. Uchimura, H. Ieyama, and K. T. Wada. 1988. Detection of induced triploid scallop, Chlamys nobilis, by DNA microfluorometry with DAPI staining. Aquaculture 69: 201-209.

Komaru, A, K. Konishi, K. Kawamura, and H. Sakai. 1998. Morphological remarks of a Corbicula species collected in Saga Prefecture, Japan. Bull. Nat. Res. Inst. Aquacult. 27 (in press).

Kuroda, T., 1938. Studies on Japanese Corbicula. Venus 8: 21-36.

Mayr, E., and P. D. Ashlock. 1991. The species category. Pp. 23-38 in Principles of Systematic Zoology, 2nd edition. McGraw-Hill, New York.

Morton, B., 1986. Corbicula in Asia - an updated synthesis. Am. Malacol. Bull. 2: 113-124.

Miyazaki, I. 1936. On the development of bivalves belonging to the genus Corbicula. Bull. Jpn. Soc. Sci. Fish. 5: 249-254. (In Japanese with English abstract.)

O'Foighil, D., and C. Thiriot-Quievreux. 1991. Ploidy and pronuclear interaction in northern Pacific Lasaea clones (Mollusca: Bivalvia) Biol. Bull. 181: 222-231.

Okamoto, A., and B. Arimoto. 1986. Chromosomes of Corbicula japonica, C. sandai and C. (Corbicula) leana (Bivalvia: Corbiculidae). Venus 45: 194-202.

Sakai, H., K. Kamiyama, S. R. Jeon, and M. Amio. 1994. Genetic relationship among three species of freshwater bivalves genus Corbicula (Corbiculidae) in Japan. Nippon Suisan Gakkaishi 60: 605-610.
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Article Details
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Title Annotation:deoxyribonucleic acid
Author:Komaru, Akira; Konishi, Kooichi; Nakayama, Ichiro; Kobayashi, Takanori; Sakai, Harumi; Kawamura, Kou
Publication:The Biological Bulletin
Date:Dec 1, 1997
Words:1342
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