Printer Friendly

Hepatitis E virus infection in sheltered homeless persons, France.

Initially considered a leading cause of acute hepatitis in tropical and subtropical countries, hepatitis E virus (HEV) is endemic to industrialized countries (1). Although substantial data indicate that HEV infection is a porcine zoonosis, more information is needed about the epidemiology and transmission of this virus in industrialized countries (1-3). Homeless persons are at higher risk than other persons for viral hepatitis (A, B, and C) because their lifestyle might include injection drug use (IDU) and poor hygiene (4), but data on HEV prevalence among them are scarce (5,6). In Marseille in southeastern France, [approximately equal to] 1,500 persons are homeless (4). Since 2000, shelter-based surveys have been conducted yearly to monitor infectious diseases in homeless persons (4). This work determined the prevalence of HEV infection in this population.

The Study

The surveys were reviewed and approved by the Institutional Review Board (CCPPCRB99/76) (Comite de Protection des Personnes Sud-Mediterranee II; www.cppsudmed2.fr/) and the Ethics Committee of the Medical School, University of the Mediterranean, Marseille). Participating homeless persons were examined by a physician and interviewed by using a standardized questionnaire, and serum samples were collected from each participant for laboratory testing. Epidemiologic, clinical, and biologic data that were collected varied from 1 year to another.

Serum samples collected from 490 homeless persons in 2003, 2005, and 2006 in 2 shelters in Marseille (online Appendix Table, www.cdc.gov/EID/content/16/11/1761-appT. htm) were tested retrospectively for immunoglobulin (Ig) G and IgM (EIAgen HEV kits; Adaltis Italia SpA, Rome, Italy) against HEV and for HEV RNA by using an in-house real-time reverse transcription-PCR specific for open reading frame 2 (7). HEV RNA sequencing was performed when HEV RNA was detected, and genotype was assigned through phylogenetic analysis of open reading frame 2 partial sequences (7). Serologic testing for hepatitis A, B, and C and for HIV were performed by using Axsym Abbott assays (Abbott Diagnostics Division, Wiesbaden, Germany). Statistical analysis was performed by using STATA version 10.1 software (StataCorp, College Station, TX, USA). Pearson x2 test, Fisher exact test, Kruskal-Wallis test, or logistic regression model were used when appropriate.

Mean [+ or -] SD age of homeless persons was 43 [+ or -] 14 years, and their mean [+ or -] SD duration of homelessness was 49 [+ or -] 84 months. Most (96.3%) were men and were born in North Africa (40.2%) or in France (33.3%) (online Appendix Table). Previous or ongoing IDU was reported for 4/176 (2.3%). Overall prevalence of anti-HEV IgG and IgM was 11.6% (95% confidence interval [CI] 8.9%-14.8%) (57/490) and 2.5% (95% CI 1.3%-4.2%) (12/490), respectively. Mean optical density ratio (optical density/cutoff value) was 3.0 (range 1.1-6.9) and 2.0 (range 1.1-4.6) for IgG and IgM, respectively. Three (0.6%; 95% CI 0.1%-1.8%) homeless persons were concurrently positive for HEV IgM and IgG, whereas 9 (1.8%; 95% CI 0.8%-3.5%) were positive only for IgM and 54 (11%; 95% CI 8.4%-14.1%) were positive only for IgG.

HEV RNA was detected in 1 homeless person, a 50year-old man from Romania concurrently seronegative for HEV IgM and IgG and for hepatitis B and C viruses. He reported excessive alcohol intake but no IDU. HEV genotype was 3f (Figure), and sequence analysis showed 98% nt identity with sequences previously recovered from persons in Spain and France. Alanine aminotransferase (ALT) level had been assessed in only 2/12 HEV IgM-positive homeless persons and was elevated in 1 person (177 IU/L), in association with an increased [gamma]-glutamyl transferase level (788 IU/L). Among the 19 homeless persons sampled in 2 different years, 1 seroconverted; he was seronegative for HEV IgM and IgG in 2005 then positive in 2006 (optical density ratio 1.14 and 4.3, respectively). Results of HEV RNA testing were negative in both serum samples, and ALT level had not been tested.

The prevalence of HEV IgG or IgM in homeless persons did not differ by year of survey; sex; place of birth; or serologic status for hepatitis A, B, or C viruses (online Ap pendix Table). In addition, mean age, duration of homelessness, and ALT, aspartate aminotransferase, and y-glutamyl transferase levels did not differ among homeless persons who were positive or negative for HEV antibodies (Table). In the univariate analysis, previous or ongoing IDU (3/4 vs. 19/172; p = 0.006), HIV seropositivity (2/3 vs. 16/183; p = 0.03), and having scabies (6/20 vs. 48/462; p = 0.02) were significantly associated with HEV IgG. In multivariate analysis that used variables that were statistically significant in the univariate analysis as covariates, only IDU was independently associated with anti-HEV IgG (adjusted odds ratio 26.3, 95% CI 2.5-267.1; p = 0.006).

[FIGURE OMITTED]

Conclusions

We found that 11.6% (95% CI 8.9%-14.8%) of homeless persons in Marseille were positive for HEV IgG, whereas 2.6% (95% CI 1.3%-4.2%) had HEV IgM or HEV RNA, indicating recent or ongoing HEV infection. The HEV IgG prevalence is similar to that previously found (6) in homeless persons in Los Angeles, California, USA (13.5%), and much lower than that found in 98 homeless children in Cochabamba, Bolivia (66.3%) (5). This prevalence falls between seroprevalences recently assessed among blood donors in northern (3.2%) and southwestern (16.6%) France (8,9).

These comparisons should take into account the use of different serologic assays, in addition to differences in epidemiologic settings. Indeed, substantial differences have been observed regarding the performances of some HEV IgG tests (10). Moreover, the sensitivity and specificity of the HEV IgG assay used in our study have not been previously evaluated, which warrants a cautious interpretation of the results. A preliminary study conducted in 2008 of 194 blood donors in Marseille with the same assay as that used in the present study found that the prevalence of HEV IgG was 9% (P. Gallian, unpub. data), which is similar to the seroprevalence we found in homeless persons. The HEV IgM assay we used showed good performance in patients with HEV genotype 3 infections in reference to PCR testing (11). Thus, sensitivity, specificity, and negative predictive value were 90%, 100%, and 98.8%, respectively. In addition, sensitivities and specificities of this test and of the HEV IgM ELISA 3.0 (MP Diagnostics, Singapore) did not differ significantly.

Although based on a small subset of homeless persons, our finding of the association of IDU with serologic results indicating past HEV infection is intriguing. This result is surprising because the proportion of IDUs in our homeless population was low (2.3%) and much lower than proportions previously reported (9%-83% (12-15). HEV IgG prevalence of 2.2%-62% has been described in IDUs, but a significant difference with the control group was found in only 2 of these studies (12-15). HEV IgG prevalence in Sweden was 62% (21/34) in patients who acquired acute hepatitis B through IDU compared with 25% (9/36) in patients with sexually acquired hepatitis B (p<0.005) (13). Moreover, in Italy, the prevalence was 5.4% (16/179) in IDUs compared with 2.6% (49/1,889) in the general population (p<0.00001) (12). In summary, our data indicate that HEV infection occurs in homeless persons, and further studies are needed to determine whether IDU is responsible for HEV transmission.

This study was supported by the Conseil General des Bouches-du-Rhone (Conseil General 13), France, and the Programme Hospitalier de Recherche Clinique, Marseille, France.

References

(1.) Dalton HR, Bendall R, Ijaz S, Banks M. Hepatitis E: an emerging infection in developed countries. Lancet Infect Dis. 2008;8:698-709. DOI: 10.1016/S1473-3099(08)70255-X

(2.) Lu L, Li C, Hagedorn CH. Phylogenetic analysis of global hepatitis E virus sequences: genetic diversity, subtypes and zoonosis. Rev Med Virol. 2006;16:5-36. DOI: 10.1002/rmv.482

(3.) Teo CG. Hepatitis E indigenous to economically developed countries: to what extent a zoonosis? Curr Opin Infect Dis. 2006;19:460-6. DOI: 10.1097/01.qco.0000244052.61629.49

(4.) Badiaga S, Raoult D, Brouqui P. Preventing and controlling emerging and reemerging transmissible diseases in the homeless. Emerg Infect Dis. 2008;14:1353-9. DOI: 10.3201/eid1409.080204

(5.) Leon P, Venegas E, Bengoechea L, Rojas E, Lopez JA, Elola C, et al. Prevalence of infections by hepatitis B, C, D and E viruses in Bolivia. Rev Panam Salud Publica. 1999;5:144-51. DOI: 10.1590/ S1020-49891999000300002

(6.) Smith HM, Reporter R, Rood MP, Linscott AJ, Mascola LM, Hogrefe W, et al. Prevalence study of antibody to ratborne pathogens and other agents among patients using a free clinic in downtown Los Angeles. J Infect Dis. 2002;186:1673-6. DOI: 10.1086/345377

(7.) Kaba M, Davoust B, Marie JL, Barthet M, Henry M, Tamalet C, et al. Frequent transmission of hepatitis E virus among piglets in farms in Southern France. J Med Virol. 2009;81:1750-9. DOI: 10.1002/ jmv.21553

(8.) Boutrouille A, Bakkali-Kassimi L, Cruciere C, Pavio N. Prevalence of anti-hepatitis E virus antibodies in French blood donors. J Clin Microbiol. 2007;45:2009-10. DOI: 10.1128/JCM.00235-07

(9.) Mansuy JM, Legrand-Abravanel F, Calot JP, Peron JM, Alric L, Agudo S, et al. High prevalence of anti-hepatitis E virus antibodies in blood donors from South West France. J Med Virol. 2008;80:28993. DOI: 10.1002/jmv.21056

(10.) Bendall R, Ellis V, Ijaz S, Ali R, Dalton H. A comparison of two commercially available anti-HEV IgG kits and a re-evaluation of anti-HEV IgG seroprevalence data in developed countries. J Med Virol. 2010;82:799-805. DOI: 10.1002/jmv.21656

(11.) Legrand-Abravanel F, Thevenet I, Mansuy JM, Saune K, Vischi F, Peron JM, et al. Good performance of immunoglobulin M assays in diagnosing genotype 3 hepatitis E virus infections. Clin Vaccine Immunol. 2009;16:772-4. DOI: 10.1128/CVI.00438-08

(12.) Gessoni G, Manoni F. Hepatitis E virus infection in north-east Italy: serological study in the open population and groups at risk. J Viral Hepat. 1996;3:197-202. DOI: 10.1111/j.1365-2893.1996.tb00095.x

(13.) Sylvan SP. The high rate of antibodies to hepatitis E virus in young, intravenous drug-abusers with acute hepatitis B-virus infection in a Swedish community: a study of hepatitis markers in individuals with intravenously or sexually acquired hepatitis B virus infection. Scand J Infect Dis. 1998;30:429-30.

(14.) Christensen PB, Engle RE, Jacobsen SE, Krarup HB, Georgsen J, Purcell RH. High prevalence of hepatitis E antibodies among Danish prisoners and drug users. J Med Virol. 2002;66:49-55. DOI: 10.1002/jmv.2110

(15.) Thomas DL, Yarbough PO, Vlahov D, Tsarev SA, Nelson KE, Saah AJ, et al. Seroreactivity to hepatitis E virus in areas where the disease is not endemic. J Clin Microbiol. 1997;35:1244-7.

Author affiliations: Centre Hospitalo-Universitaire Timone, Marseille, France (M. Kaba, P. Colson); Universite de la Mediterranee, Marseille (M. Kaba, P. Brouqui, H. Richet, S. Badiaga, D. Raoult, P. Colson); Hopital Nord, Marseille (P. Brouqui, S. Badiaga); and Etablissement Francais du Sang Alpes-Mediterranee, Marseille (P. Gallian)

DOI: 10.3201/eid1611.091890

Address for correspondence: Philippe Colson, Pole des Maladies Infectieuses et Tropicales Cliniques et Biologiques, Federation de Bacteriologie-Hygiene-Virologie, Centre Hospitalo-Universitaire Timone, 264 rue Saint-Pierre, 13385 Marseille CEDEX 05, France; email: philippe.colson@ap-hm.fr

Dr Kaba works at Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Facultes de Medecine et de Pharmacie, Universite de la Mediterranee, and at the Pole des Maladies Infectieuses et Tropicales Clinique et Biologique, Federation de Bacteriologie-Hygiene-Virologie, Centre HospitaloUniversitaire Timone, in Marseille, France. His primary field of interest is viral hepatitis.
Table. Age, duration of homelessness, and liver biochemical
test results for 490 homeless persons, Marseilles,
France, 2003, 2005, and 2006 *

                                             HEV IgM

Variable                  Positive          Negative       p value

Age, y, mean           37 [+ or -] 12    43 [+ or -] 14     0.13
[+ or -] SD

Duration of            39 [+ or -] 38    49 [+ or -] 85     0.36
homelessness,
mo, mean
[+ or -] SD

Liver
biochemical
tests, ([dagger])
mean [+ or -] SD,
IU/L

  Alanine
  aminotransferase
  levels
  ([double
  dagger])            93 [+ or -] 119    32 [+ or -] 31     0.99

  Aspartate
  aminotransferase
  levels
  ([section])          49 [+ or -] 54    34 [+ or -] 43     0.99

  [gamma]-glutamyl
  transferase
  ([paragraph])       401 [+ or -] 547   65 [+ or -] 137    0.92

                                             HEV IgG

Variable                  Positive          Negative       p value

Age, y, mean           45 [+ or -] 15    43 [+ or -] 14     0.24
[+ or -] SD

Duration of            48 [+ or -] 78    49 [+ or -] 85     0.28
homelessness,
mo, mean
[+ or -] SD

Liver
biochemical
tests, ([dagger])
mean [+ or -] SD,
IU/L

  Alanine
  aminotransferase
  levels
  ([double
  dagger])             36 [+ or -] 32    32 [+ or -] 33     0.49

  Aspartate
  aminotransferase
  levels
  ([section])          34 [+ or -] 32    34 [+ or -] 44     0.79

  [gamma]-glutamyl
  transferase
  ([paragraph])       76 [+ or -] 136    68 [+ or -] 147    0.44

* HEV, hepatitis E virus; Ig, immunoglobulin.

([dagger]) Assessed only in 2005 for 209 homeless persons.

([double dagger]) Data missing for 11 persons.

([section]) Data missing for 10 persons.

([paragraph]) Data missing for 9 persons.
COPYRIGHT 2010 U.S. National Center for Infectious Diseases
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 2010 Gale, Cengage Learning. All rights reserved.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:DISPATCHES
Author:Kaba, Mamadou; Brouqui, Philippe; Richet, Herve; Badiaga, Sekene; Gallian, Pierre; Raoult, Didier; C
Publication:Emerging Infectious Diseases
Article Type:Report
Geographic Code:4EUFR
Date:Nov 1, 2010
Words:2171
Previous Article:Extended-spectrum [beta]-lactamase--producing Escherichia coli in neonatal care unit.
Next Article:Enterovirus 71 infection with central nervous system involvement, South Korea.
Topics:

Terms of use | Privacy policy | Copyright © 2020 Farlex, Inc. | Feedback | For webmasters