Helicobacter pylori Infection and Gastric Dysbiosis: Can Probiotics Administration Be Useful to Treat This Condition?
Helicobacter pylori (Hp) is a Gram-negative, spiral-shaped, flagellated bacterium belonging to Proteobacteria phylum with a strong capacity of surviving in the harsh acid milieu of the stomach. Hp is responsible for one of the most widespread infections in the world [1, 2], and the number of infected subjects is still very high worldwide. The prevalence exceeds 50% of the population in some areas of the world, like South and East Europe, South America, and Asia. Low socioeconomic status, bad level of education, and poor hygienic conditions are the major related risk factors. Notably, about one-third of the adults are colonized in North Europe and North America .
Several recent evidence have highlighted that Hp can modify the composition of gastric microbiota and the resulting changes can play a role in the development of Hp-related diseases. Still, the interaction between the host, microbiota, and Hp in the pathogenesis of these conditions has to be fully elucidated .
Hp colonization can cause chronic gastritis, peptic ulcer, atrophic gastritis, gastric adenocarcinoma, and mucosa-associated lymphoid tissue lymphoma (MALT) [5, 6]. Hp gastritis is considered an infectious disease regardless of symptoms and disease stage, and eradication therapy is strongly recommended . However, a rapid emergence of antibiotic-resistant bacteria is becoming one of the world's most critical public health problems, and thus, the choice of therapeutic options for the treatment of Hp infection faces this dilemma .
In this context, the use of probiotics, defined as "live microorganisms which when administrated in adequate amounts confer a health benefit on the host"  can be helpful for their antibacterial activity against Hp and for the interaction with the complex ecosystem of the host .
The beneficial properties of probiotics on the host microbiological environment can be associated with their potential effects on digestive microflora and gut immune system that include their ability to compete with gut pathogens, to increase IgA secretion, to modulate cytokine mRNA expression and secretion, to stimulate mucin, bacteriocin, and lactic acid production, and to modulate microbiota growth [11-13].
The aim of this review is to provide an overview of the changes in gastric microbiota composition during Hp infection and then to assess the potential role of probiotics in Hp-induced dysbiosis and eradication. A critical appraisal of the clinical research evidence on the data regarding Hp and gastric microbiota composition and on the probiotics effectiveness to treat Hp infection and to prevent antimicrobial therapy side effects was made. The search was limited to full manuscripts in English language.
2. Helicobacter pylori and Gastric Microbiota Composition
The stomach has always been considered a sterile organ. It is not surprising that for long, it was believed that low pH of the gastric lumen and peristalsis contributed to create an adverse environment for bacterial survival and stable microbial colonization of this organ. However, in 1983, the discovery of Hp by Marshall and Warren  gave the input to a period of progressive discoveries in the field of gastric infection and ensured a breakthrough in understanding gastric microecological environment. The improvement in microbial detection techniques has been crucial. The initial analysis was performed using culture-based methods, which harbor various limitations; in particular, they regard a large amount of bacteria that are still considered "unculturable" due to growth resistance in conventional culture media, need for particular environment conditions, low bacterial growth rate, and interaction with other bacteria or their secreted substrates . Taken together or individually, these factors can determine an incomplete and limited representation of the complex gastric bacterial community improperly showing a similar gastric microbiota composition in Hp patients compared with healthy subjects [16, 17]. Conversely, the most recent molecular techniques which allow an in-depth study of the gastric microbiota have highlighted a significant difference in microbiota composition between Hp-positive and healthy subjects. The technique consists in sequencing the ribosomal gene 16S rRNA that contains 9 variable regions, which are present in all bacteria and are similar in the microorganisms of the same phyla. Technically, this type of analysis allows the recognition of different bacterial species through the analysis of the genoma, which makes it difficult to evaluate the vitality of microorganisms. To avoid this drawback, it is preferable to use bacterial RNA instead of DNA .
Currently, we know that, in healthy subjects, gastric microbiota is composed mainly of Firmicutes, Bacteroidetes, Proteobacteria, and Actinobacteria at phyla level. The most present genera are Streptococcus, followed by Veillonella, Prevotella, Fusobacterium, and Rothia [17, 19]. The analysis performed on gastric juice and biopsies has suggested that the density of gastric microbiota is lower than in other parts of the gastrointestinal (GI) tract, counting about [10.sup.1]-[10.sup.3] CFU/ml .
The presence of bacteria in the stomach is possible due to the progressive shift of pH from the gastric lumen (pH 1-2) to the mucosal surface (pH 6-7) coated with mucus which is actively secreted by gastric glands. This pH gradient permits the development of different environments that allow the growth of microorganisms, being the mucosal surface the more hospitable gastric area . Hp colonization of gastric mucosa alters this gastric habitat by deconstructing the mucous layer and by alkalinization of gastric juice .
In Hp-positive subjects, molecular analysis showed an overall decrease in bacterial diversity with the absolute prevalence at phyla level of Proteobacteria, followed by Firmicutes, Bacteroidetes, and Actinobacteria. In the genus subanalysis, excluding Hp which turns out to be the most represented species, Streptococcus are commonly the second detected bacteria [23, 24]. An interesting study by Andersson et al., evaluating samples from both, Hp-positive and healthy subjects, showed that the presence of Hp causes a reduction of microbial diversity. Only 33 phylotypes were found in Hp subjects compared to a rich and diversified assortment of gastric microorganism of about 262 phylotypes in healthy people, highlighting this difference .
In order to establish whether gastric microbiota alterations found in concomitant with Hp infection were induced by hypochlorhydria, Parsons et al. analyzed gastric microbiota composition in various hypochlorhydric conditions and in healthy subjects. As previously described, also in this study the stomach of healthy subjects contains the widest bacterial diversity compared with other groups. Interestingly, PPI group shows a microbiota characterization which is similar in a healthy stomach, despite the hypochlorhydric status due to the drug use. A significant difference in microbial diversity was found comparing healthy subjects with Hp-related conditions and an interesting data emerge from the comparison between Hp gastritis and Hp-related atrophic gastritis. In fact, no significant differences were found between these two conditions, suggesting that, in the Hp subgroup patients, the role of bacteria may be superior in inducing changes in the composition of gastric microbiota compared with hypochlorhydria .
The changes in gastric microbiota due to Hp infection can be related to intrinsic properties of bacteria. In fact, Hp is provided with oxidase, catalase, and urease activities. The urease is a metalloenzyme able to convert urea into ammonia and bicarbonate that is responsible for the local increase in gastric pH and the safe passage into the gastric lumen. Hp flagella enable the pathogen to pass through the mucus layer driven by the pH gradient, thus permitting gastric mucosal invasion. Once established, Hp exposes adhesins (HopQ, HopP, and HopS) that permit a receptor-specific close adhesion to gastric epithelial cells and consequently the expression of CagA and VacA virulence factors which mediate the cytotoxic activity [26-28]. Mucosal damage attracts polymorphonuclear and other immune cells that likely contribute to gastric damage by producing cytokines and other proinflammatory substances . Probably, these factors creating a hostile environment make difficult the survival of the other bacteria, allowing the establishment of gastric dysbiosis.
3. Helicobacter pylori Eradication and Probiotics Administration
According to the Maastricht V Consensus Report, the standard triple therapy, proton pump inhibitor (PPI) + amoxicillin (AMX) + clarithromycin (CLR), is considered the first-line empirical treatment in low CLR resistance areas. When the regional resistance to CLR is high, considering 15% of population as the verge, nonbismuth quadruple therapy with PPI + AMC + metronidazole (MTZ) + CLR is recommended. In all these cases, a bismuth quadruple therapy can be used as an alternative and it is recommended as first-line treatment in areas with high dual CLR-MTZ resistance. The benefit of Hp eradication has been demonstrated in conditions like peptic ulcer disease, MALT lymphoma, iron deficiency anemia, idiopathic thrombocytopenic purpura, and vitamin B12 deficiency . Antibiotic resistance is leading to increasing estimates of treatment failures, as demonstrated by the raising rate of CLR-resistant strains that has reached 40-50% in some areas of the world .
Based on the literature data (Table 1), it emerges that some probiotics associated with antibiotic therapy can improve Hp eradication rate and moreover, can reduce deleterious side effects due to antimicrobial therapy such as nausea, vomiting, diarrhea, abdominal pain, bloating, and taste disturbance that occasionally are responsible for the withdrawal of the treatment [31, 32].
The role of Lactobacillus strain administration in the treatment of Hp infection and in preventing antimicrobial therapy side effects has been well documented. The supplementation of these probiotics can directly reduce Hp growth rate and Hp colonization. In a prospective, randomized, controlled trial by Ojetti et al., adding a probiotic (L. reuteri 1 x [10.sup.8] CFU tid for 14 days) to levofloxacin-based second-line therapy showed an additional 20% eradication rate compared with antibiotic treatment alone, followed by a consistent reduction of symptoms such as diarrhea and nausea . Armuzzi et al. enrolled 120 asymptomatic Hp-positive subjects which were randomly assigned to two treatment groups: one receiving anti-Hp triple therapy (PPI 40 mg bid, CLR 500 mg bid, and tinidazole 500 mg bid) and the other receiving the same antibiotic therapy associated with Lactobacillus GG-containing probiotic (6 x 109 of viable bacteria) bid for 14 days. The analysis documented an improvement in gastrointestinal symptoms in the probiotic supplementation group with a significant reduction in taste disturbances (p = 0.007), bloating (p = 0.01), and diarrhea (p = 0.02). On the other hand, no significant differences in eradication rates were reported (group 1 vs. group 2; PP: 80.7% vs. 80%, p = 0.9) . Excellent results can be obtained also by providing drinkable food supplements containing Lactobacilli or its culture supernatant [35-38].
The beneficial effects of Bifidobacterium administration were analyzed by Chitapanarux et al. in a double-blind, placebo-controlled trial using B. longum in addition to the standard triple therapy. The results showed significant beneficial effect on Hp eradication rate (PP: 28/30, 93.33% vs. PP: 22/30, 73.33%, p = 0.04) and on diarrhea frequency reduction (25% vs. 3.23%, p = 0.027) with no significant outcomes on nausea (18.75 vs. 12.90%), taste disturbance (15.63% vs. 12.90%), and epigastric pain (6.25 vs. 3.23%) . However, in a previous study by Yasar et al. that included 76 histopathologically proven Hp-positive patients, the addition of Bifidobacterium DN-173-containing yogurt to the standard triple therapy for 14 days resulted in the eradication rate of 66% compared to 53% with antibiotic therapy alone. Anyhow, the increase of Hp eradication rate was not statistically significant .
Saccharomyces boulardii, a yeast probiotic, was particularly effective in reducing side effects of the eradication therapy. Song et al. reported that adding this probiotic to CLR and AMX-based triple therapy for 4 weeks reached 85.4% eradication rate compared to 80% in the absence of probiotic. Gastrointestinal side effects, in particular diarrhea, were more common in the latter group (p < 0.05) . A meta-analysis by Szajewska et al. including eleven RCTs (2200 participants, among them 330 children) showed that S. boulardii significantly increased Hp eradication rate but below the aimed level. However, this probiotic significantly reduced side effects, in particular diarrhea and nausea .
Based on the properties of probiotics, it is plausible that a mixture of strains can improve Hp eradication rate. In a trial of Du et al., a multistrain probiotic containing Lactobacillus acidophilus, Streptococcus faecalis, and Bacillus subtilis was administered to the patients for two weeks before antibiotic treatment or in another group for two weeks after the eradication therapy. Both schedules were more effective in eradicating Hp infection than the triple therapy alone (81.6% and 82.4% vs. 61.5%), but there was no statistical significance regarding the incidence of side effects . A meta-analysis by Wang et al. which included ten trials (1469 subjects) showed that probiotic addition using compounds containing Lactobacillus and Bifidobacterium strains significantly improves both Hp eradication rates and reduces the incidence of antimicrobial therapy side effects. Moreover, the advantage of probiotics supplementation was demonstrated independently of the type of eradication therapy used in the trial, despite the bismuth-containing therapy which could represent a potential failure due to the inhibitory activity of bismuth against the probiotics . Furthermore, a successive meta-analysis by McFarland et al. confirmed that multistrain probiotics can be helpful as an adjunct therapy for Hp eradication and in preventing eradication side effects, but they concluded that not all mixtures were equally effective . Finally, Lau et al. confirmed the utility of Lactobacillus, Bifidobacterium, Saccharomyces, and probiotics mixtures in the treatment of Hp infection in both adults and children, in Asian and non-Asian population .
At present, the use of probiotics in children to treat Hp infection is not clear. In the meta-analysis previously mentioned by Wang et al., the children subgroup analysis showed no probiotic efficacy on eradication rates and reduction of undesirable side effects . Pacifico et al. pointed out the controversial efficacy of probiotics use in these patients. Seven trials comprising heterogeneous antibiotic and probiotic therapies showed a general benefit in eradication rates due to probiotic supplementation, but in two only, the results were statistically significant, thus concluding that no convincing evidence was present to support the use of probiotics with triple therapy in children . Moreover, in 2015, the Latin American Expert group consensus stated that at that time, there was a lack of sufficient evidence to recommend the administration of probiotics in this area . However, other evidences suggest that some probiotics like S. boulardii or L. casei alone or multistrains (L. acidophilus + B. bifidum, B. mesentericus + C. butyricum + S. faecalis) can be helpful to treat Hp and prevent side effects due to the eradication therapy in pediatric patients, recommending their use [49-51].
The beneficial effects of these probiotics against Hp infection can be related to their intrinsic properties that can depend on probiotic species (Figure 1). Lactobacilli have been shown to possess the following properties:
(1) Production of antimicrobial substances, as bacteriocins that are synthesized by ribosomes and secreted by several bacteria including Lactobacilli. These substances are provided with antimicrobial activity and represent an important and successful weapon against other microbial species like Hp [13, 52]. Some bacteriocins, such as nisin, pediocin, acidocin, and lacticin, contribute to homeostasis of the complex GI tract ecosystem [53-55].
(2) Capacity to survive in the gastric acidic environment by inducing lactic and volatile acids' production which has an inhibitory effect on Hp growth. The effects of supplementation with L. salivarius have been documented in a mouse model study, and its efficacy has been related to the high amount of lactic acid produced by the bacterium that interferes with the urease activity of the pathogen . Furthermore, in vitro, L. salivarius is able to reduce gastric inflammation by modulating local cytokine secretion, in particular of IL-8 directly related to neutrophil recruitment and mucosal inflammation, probably as a response to the suppressed secretion of CagA virulence factor .
(3) Ability to adhere at gastric and duodenal cells and thus to perform a competitive action against pathogens. Mukai et al. found that two L. reuteri strains, JCM 1081 and TM'05, were able to bind specific membrane glycolipids and therefore occupy the potential site of Hp adhesion, inhibiting this pathogen gastric colonization .
Similar mechanisms of action were also described for other probiotics. Bifidobacterium strains are able to produce antimicrobial substances that can inhibit Hp growth, thus improving eradication rates [10, 44, 59]. Interestingly, a study in mice by Yu et al. suggested that a probiotic mixture containing B. longum, L. acidophilus, and E. faecalis can ameliorate Hp gastritis reducing the inflammatory response by inhibiting cytokine secretion such as IL-8, TNF-[alpha], G-CSF, and GM-CSF . Conversely, S. boulardii can reduce Hp adhesion at gastric and duodenal cells through its neuraminidase activity able to selectively reduce the expression of [alpha] (2-3)-linked sialic acid on cell surface, a target of Hp adhesions .
Our review highlights how some probiotic strains can improve Hp eradication rates and prevent antimicrobial therapy side effects, likely due to the capabilities of these microorganisms to directly act against Hp, restoring a healthy microbiota. In Italy, the mean cost of probiotics in addition to antimicrobial therapy based on the studies present in this review is about 2 [euro] per day of treatment per patient. In our opinion, this cost is widely justified by the reduction of side effects due to antibiotic therapy and the increased eradication rate which reduces the need for a second-line treatment. Therefore, the administration of probiotics may be considered an adjunctive treatment especially when Hp eradication fails. However, not all probiotics are alike useful, and probably, the curative effects are strain dependent. Additional studies are necessary to better understand their role in Hp infection, particularly when using the updated eradication schedules.
At present, few studies or meta-analysis are available on a direct comparison between beneficial effects of different probiotics species during antimicrobial therapy. However, according to the available data and to the Maastricht V Consensus Report statement "Certain probiotics may have a beneficial effect on Hp eradication" , we recommend the use of Lactobacillus, Bifidobacterium, and Saccharomyces strains. Nevertheless, in our opinion, more trials with probiotics should be tailored to the state of gastric microbiota composition before and after Hp eradication therapy, inasmuch as it could explain the role of various bacteria in the development of gastric diseases.
Conflicts of Interest
The authors declare that they have no conflicts of interest.
Giovanni Bruno and Giulia Rocco drafted the manuscript with input from all authors. Piera Zaccari critically revised the manuscript. Barbara Porowska, Maria Teresa Mascellino, and Carola Severi provided scientific guidance.
 J. G. Kusters, A. H. van Vliet, and E. J. Kuipers, "Pathogenesis of Helicobacter pylori infection," Clinical Microbiology Reviews, vol. 19, no. 3, pp. 449-490, 2006.
 W. D. Chey, G. I. Leontiadis, C. W. Howden, and S. F. Moss, "ACG clinical guideline: treatment of Helicobacter pylori infection," American Journal of Gastroenterology, vol. 112, no. 2, pp. 212-239, 2017.
 L. H. Eusebi, R. M. Zagari, and F. Bazzoli, "Epidemiology of Helicobacter pylori infection," Helicobacter, vol. 19, no. 1, pp. 1-5, 2014.
 C. Schulz, N. Koch, K. Schutte, D. H. Pieper, and P. Malfertheiner, "H. pylori and its modulation of gastrointestinal microbiota," Journal of Digestive Diseases, vol. 16, no. 3, pp. 109-117, 2015.
 M. K. Sanders and D. A. Peura, "Helicobacterpylori-associated diseases," Current Gastroenterology Reports, vol. 4, no. 6, pp. 448-454, 2002.
 L. E. Wroblewski, R. M. Peek, and K. T. Wilson, "Helicobacter pylori and gastric cancer: factors that modulate disease risk," Clinical Microbiology Reviews, vol. 23, no. 4, pp. 713-739, 2010.
 K. Sugano, J. Tack, E. J. Kuipers et al., "Kyoto global consensus report on Helicobacter pylori gastritis," Gut, vol. 64, no. 9, pp. 1353-1367, 2015.
 I. Thung, H. Aramin, V. Vavinskaya et al., "Review article: the global emergence of Helicobacter pylori antibiotic resistance," Alimentary Pharmacology and Therapeutics, vol. 43, no. 4, pp. 514-533, 2016.
 Food and Agricultural Organization of the United Nations and World Health Organization, Health and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria, World Health Organization, Geneva, Switzerland, 2001.
 M. Gotteland, O. Brunser, and S. Cruchet, "Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori?," Alimentary Pharmacology and Therapeutics, vol. 23, no. 8, pp. 1077-1086, 2006.
 K. L. Erickson and N. E. Hubbard, "Probiotic immunomodulation in health and disease," Journal of Nutrition, vol. 130, no. 2, pp. 403S-409S, 2000.
 G. T. Rijkers, S. Bengmark, P. Enck et al., "Guidance for substantiating the evidence for beneficial effects of probiotics: current status and recommendations for future research," Journal of Nutrition, vol. 140, no. 3, pp. 671S-676S, 2010.
 L. Boyanova, G. Gergova, R. Markovska, D. Yordanov, and I. Mitov, "Bacteriocin-like inhibitory activities of seven Lactobacillus delbrueckii subsp. bulgaricus strains against antibiotic susceptible and resistant Helicobacter pylori strains," Letters in Applied Microbiology, vol. 65, no. 6, pp. 469-474, 2017.
 J. R. Warren and B. Marshall, "Unidentified curved bacilli on gastric epithelium in active chronic gastritis," The Lancet, vol. 1, no. 8336, pp. 1273-1275, 1983.
 S. R. Vartoukian, R. M. Palmer, and W. G. Wade, "Strategies for culture of 'unculturable' bacteria," FEMS Microbiology Letters, vol. 309, no. 1, pp. 1-7, 2010.
 L. Engstrand and M. Lindberg, "Helicobacter pylori and the gastric microbiota," Best Practice and Research Clinical Gastroenterology, vol. 27, no. 1, pp. 39-45, 2013.
 A. Sheh and J. G. Fox, "The role of the gastrointestinal microbiome in Helicobacter pylori pathogenesis," Gut Microbes, vol. 4, no. 6, pp. 505-531, 2013.
 C. Schulz, K. Schutte, N. Koch et al., "The active bacterial assemblages of the upper GI tract in individuals with and without Helicobacter infection," Gut, vol. 67, no. 2, pp. 216-225, 2018.
 G. Nardone and D. Compare, "The human gastric microbiota: is it time to rethink the pathogenesis of stomach diseases?," United European Gastroenterology Journal, vol. 3, no. 3, pp. 255-260, 2015.
 A. M. O'Hara and F. Shanahan, "The gut flora as a forgotten organ," EMBO Reports, vol. 7, no. 7, pp. 688-693, 2006.
 R. H. Hunt, M. Camilleri, S. E. Crowe et al., "The stomach in health and disease," Gut, vol. 64, no. 10, pp. 1650-1668, 2015.
 S. M. Kelly, J. R. Crampton, and J. O. Hunter, "Helicobacter pylori increases gastric antral juxtamucosal pH," Digestive Diseases and Sciences, vol. 38, no. 1, pp. 129-131, 1993.
 I. Klymiuk, C. Bilgilier, A. Stadlmann et al., "The human gastric microbiome is predicated upon infection with Helicobacter pylori," Frontiers in Microbiology, vol. 8, p. 2508, 2017.
 B. N. Parsons, U. Z. Ijaz, R. D'Amore et al., "Comparison of the human gastric microbiota in hypochlorhydric states arising as a result of Helicobacter pylori-induced atrophic gastritis, autoimmune atrophic gastritis and proton pump inhibitor use," PLoS Pathogens, vol. 13, no. 11, article e1006653, 2017.
 A. F. Andersson, M. Lindberg, H. Jakobsson, F. Backhed, P. Nyren, and L. Engstrand, "Comparative analysis of human gut microbiota by barcoded pyrosequencing," PLoS One, vol. 3, no. 7, Article ID e2836, 2008.
 A. Javaheri, T. Kruse, K. Moonens et al., "Helicobacter pylori adhesin HopQ engages in a virulence-enhancing interaction with human CEACAMs," Nature Microbiology, vol. 2, article 16189, 2016.
 S. Backert, M. Neddermann, G. Maubach, and M. Naumann, "Pathogenesis of Helicobacter pylori infection," Helicobacter, vol. 21, no. 1, pp. 19-25, 2016.
 C. Y. Kao, B. S. Sheu, and J. J. Wu, "Helicobacter pylori infection: an overview of bacterial virulence factors and pathogenesis," Biomedical Journal, vol. 39, no. 1, pp. 14-23, 2016.
 R. G. Zhang, G. C. Duan, Q. T. Fan, and S.-Y. Chen, "Role of Helicobacter pylori infection in pathogenesis of gastric carcinoma," World Journal of Gastrointestinal Pathophysiology, vol. 7, no. 1, pp. 97-107, 2016.
 P. Malfertheiner, F. Megraud, C. A. O'Morain et al., "European Helicobacter and Microbiota Study Group and consensus panel. Management of Helicobacter pylori infection-the maastricht v/florence consensus report," Gut, vol. 66, no. 1, pp. 6-30, 2017.
 C. Felley and P. Michetti, "Probiotics and Helicobacter pylori," Best Practice and Research Clinical Gastroenterology, vol. 17, no. 5, pp. 785-791, 2003.
 J. M. Vltor and F. F. Vale, "Alternative therapies for Helicobacter pylori: probiotics and phytomedicine," FEMS Immunology and Medical Microbiology, vol. 63, no. 2, pp. 153-164, 2011.
 V. Ojetti, G. Bruno, M. E. Ainora et al., "Impact of Lactobacillus reuteri supplementation on anti-Helicobacter pylori levofloxacin-based second-line therapy," Gastroenterology Research and Practice, vol. 2012, Article ID 740381, 2012.
 A. Armuzzi, F. Cremonini, V. Ojetti et al., "Effect of Lactobacillus GG supplementation on antibiotic-associated gastrointestinal side effects during Helicobacter pylori eradication therapy: a pilot study," Digestion, vol. 63, no. 1, pp. 1-7, 2001.
 P. Michetti, G. Dorta, P. H. Wiesel et al., "Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans," Digestion, vol. 60, no. 3, pp. 203-209, 1999.
 M. Gotteland and S. Cruchet, "Suppressive effect of frequent ingestion of Lactobacillus johnsonii La1 on Helicobacter pylori colonization in asymptomatic volunteers," Journal of Antimicrobial Chemotherapy, vol. 51, no. 5, pp. 1317-1319, 2003.
 I. Sakamoto, M. Igarashi, K. Kimura, A. Takagi, T. Miwa, and Y. Koga, "Suppressive effect of Lactobacillus gasseri OLL 2716 (LG21) on Helicobacter pylori infection in humans," Journal of Antimicrobial Chemotherapy, vol. 47, no. 5, pp. 709-710, 2001.
 F. Canducci, A. Armuzzi, F. Cremonini et al., "A lyophilized and inactivated culture of Lactobacillus acidophilus increases Helicobacter pylori eradication rates," Alimentary Pharmacology and Therapeutics, vol. 14, no. 12, pp. 1625-1629, 2000.
 T. Chitapanarux, S. Thongsawat, P. Pisespongsa, A. Leerapun, and P. Kijdamrongthum, "Effect of Bifidobacterium longum on PPI-based triple therapy for eradication of Helicobacter pylori: a randomized, double-blind placebo-controlled study," Journal of Functional Foods, vol. 13, pp. 289-294, 2015.
 B. Yasar, E. Abut, H. Kayadibi et al., "Efficacy of probiotics in Helicobacter pylori eradication therapy," Turkish Journal of Gastroenterology, vol. 21, no. 3, pp. 212-217, 2010.
 M. J. Song, D. I. Park, J. H. Park et al., "The effect of probiotics and mucoprotective agents on PPI- based triple therapy for eradication of Helicobacter pylori," Helicobacter, vol. 15, no. 3, pp. 206-213, 2010.
 H. Szajewska, A. Horvath, and M. Kolodziej, "Systematic review with meta-analysis: Saccharomyces boulardii supplementation and eradication of Helicobacter pylori infection," Alimentary Pharmacology and Therapeutics, vol. 41, no. 12, pp. 1237-1245, 2015.
 Y. Q. Du, T. Su, J. G. Fan et al., "Adjuvant probiotics improve the eradication effect of triple therapy for Helicobacter pylori infection," World Journal of Gastroenterology, vol. 18, no. 43, pp. 6302-6307, 2012.
 Z. H. Wang, Q. Y. Gao, and J. Y. Fang, "Meta-analysis of the efficacy and safety of Lactobacillus-containing and Bifidobacterium-containing probiotic compound preparation in Helicobacter pylori eradication therapy," Journal of Clinical Gastroenterology, vol. 47, no. 1, pp. 25-32, 2013.
 L. V. McFarland, Y. Huang, L. Wang et al., "Systematic review and meta-analysis: multi-strain probiotics as adjunct therapy for Helicobacter pylori eradication and prevention of adverse events," United European Gastroenterology Journal, vol. 4, no. 4, pp. 546-561, 2016.
 C. S. Lau, A. Ward, and R. S. Chamberlain, "Probiotics improve the efficacy of standard triple therapy in the eradication of Helicobacter pylori: a meta-analysis," Infection and Drug Resistance, vol. 9, pp. 275-289, 2016.
 L. Pacifico, J. F. Osborn, E. Bonci, S. Romaggioli, R. Baldini, and C. Chiesa, "Probiotics for the treatment of Helicobacter pylori infection in children," World Journal of Gastroenterology, vol. 20, no. 3, pp. 673-683, 2014.
 S. Cruchet, R. Furnes, A. Maruy et al., "The use of probiotics in pediatric gastroenterology: a review of the literature and recommendations by Latin-American experts," Paediatr Drugs, vol. 17, no. 3, pp. 199-216, 2015.
 D. Cameron, Q. S. Hock, M. Kadim et al., "Probiotics for gastrointestinal disorders: proposed recommendations for children of the Asia-Pacific region," World Journal of Gastroenterology, vol. 23, no. 45, pp. 7952-7964, 2017.
 J. R. Feng, F. Wang, X. Qiu et al., "Efficacy and safety of probiotic-supplemented triple therapy for eradication of Helicobacter pylori in children: a systematic review and network meta-analysis," European Journal of Clinical Pharmacology, vol. 73, no. 10, pp. 1199-1208, 2017.
 Y. H. Wang and Y. Huang, "Effect of Lactobacillus acidophilus and Bifidobacterium bifidum supplementation to standard triple therapy on Helicobacter pylori eradication and dynamic changes in intestinal flora," World Journal of Microbiology and Biotechnology, vol. 30, no. 3, pp. 847-53, 2014.
 T. S. Kim, J. W. Hur, M. A. Yu et al., "Antagonism of Helicobacter pylori by bacteriocins of lactic acid bacteria," Journal of Food Protection, vol. 66, no. 1, pp. 3-12, 2003.
 S. C. Yang, C. H. Lin, C. T. Sung et al., "Antibacterial activities of bacteriocins: application in foods and pharmaceuticals," Frontiers in Microbiology, vol. 5, p. 241, 2014.
 K. Selle and T. R. Klaenhammer, "Genomic and phenotypic evidence for probiotic influences of Lactobacillus gasseri on human health," FEMS Microbiology Reviews, vol. 37, no. 6, pp. 915-935, 2013.
 M. Dalmau, E. Maier, N. Mulet et al., "Bacterial membrane injuries induced by lactacin F and nisin," International Microbiology, vol. 5, no. 2, pp. 73-80, 2002.
 Y. Aiba, N. Suzuki, A. M. Kabir et al., "Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model," American Journal of Gastroenterology, vol. 93, no. 11, pp. 2097-2101, 1998.
 K. A. Ryan, A. M. O'Hara, J. P. van Pijkeren, F. P. Douillard, and P. W. O'Toole, "Lactobacillus salivarius modulates cytokine induction and virulence factor gene expression in Helicobacter pylori," Journal of Medical Microbiology, vol. 58, no. 8, pp. 996-1005, 2009.
 T. Mukai, T. Asasaka, E. Sato et al., "Inhibition of binding of Helicobacter pylori to the glycolipid receptors by probiotic Lactobacillus reuteri," FEMS Immunology and Medical Microbiology, vol. 32, no. 2, pp. 105-110, 2002.
 M. C. Collado, A. Gonzalez, R. Gonzalezet al., "Antimicrobial peptides are among the antagonistic metabolites produced by Bifidobacterium against Helicobacter pylori," International Journal of Antimicrobial Agents, vol. 25, no. 5, pp. 385-391, 2005.
 H. J. Yu, W. Liu, Z. Chang et al., "Probiotic BIFICO cocktail ameliorates Helicobacter pylori induced gastritis," World Journal of Gastroenterology, vol. 21, no. 21, pp. 6561-6571, 2015.
 S. Sakarya and N. Gunay, "Saccharomyces boulardii expresses neuraminidase activity selective for a2,3-linked sialic acid that decreases Helicobacter pylori adhesion to host cells," APMIS, vol. 122, no. 10, pp. 941-950, 2014.
Giovanni Bruno, (1) Giulia Rocco, (1) Piera Zaccari, (1) Barbara Porowska, (2) Maria Teresa Mascellino, (3) and Carola Severi (1)
(1) Department of Internal Medicine and Medical Specialties, Gastroenterology Unit, Sapienza University, Rome, Italy
(2) Department of Cardio-Thoracic, Vascular Surgery and Transplants, Sapienza University, Rome, Italy
(3) Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy
Correspondence should be addressed to Giovanni Bruno; firstname.lastname@example.org
Received 13 June 2018; Accepted 30 July 2018; Published 10 September 2018
Academic Editor: Jose A. Oteo
Caption: Figure 1: Mechanisms of action of probiotics.
Table 1: Efficacy of probiotics administration in addiction to antimicrobial therapy compared to antimicrobial therapy alone in clinical trials present in our review. Study Patients Antimicrobial Probiotic therapy Ojetti et Adults E: 20 mg bid, Lactobacillus reuteri (1 x al.  L: 500 mg bid, [10.sup.8] CFU tid) A: 1 g bid Armuzzi et Adults P: 40 mg bid, Lactobacillus GG (6 x al.  C: 500 mg bid, [10.sup.9] CFU bid) T: 500 mg bid Chitapanarux et al.  Adults E: 40 mg bid, Bifidobacterium longum (not C: 500 mg bid, specified) A: 1 g bid Bifidobacterium DN/173 010/ Yasar et Adults P: 40 mg bid, 1010 CFU/g yogurt 125 ml al.  C: 500 mg bid, A: 1 g bid Song et Adults O: 20 mg bid, Saccharomyces boulardii (3 al.  C: 500 mg bid, x [10.sup.10] CFU/g) A: 1 g bid Du et al. Adults O: 20 mg bid, Lactobacillus acidophilus  C: 500 mg bid, (5 x [10.sup.6]), A: 1 g bid Streptococcus faecalis (2.5 x [10.sup.6]), and Bacillus subtilis (5 x [10.sup.3]) tid Study Duration Eradication Side-effects improvement reduction Ojetti et 14 days Yes Yes al.  Armuzzi et 14 days No Yes al.  Chitapanarux et al.  4 weeks Yes Yes Yasar et 14 days No Yes al.  Song et 4 weeks Yes Yes al.  Du et al. 2 weeks Yes No  O: omeprazole; P: pantoprazole; E: esomeprazole; C: clarithromycin; A: amoxicillin; L: levofloxacin; T: tinidazole.