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Hantaviruses in the Czech Republic. (Letters).

To the Editor: Infections caused by hantaviruses have been known for a long time, but their causative agent was not detected until 1976 (1). These viruses of the genus Hantavirus, family Bunyaviridae, have >20 genotypes. Hantaviruses circulate in wild rodents within natural foci over Euroasia and North and South America. They cause asymptomatic persistent infections in these small mammals. Humans may acquire infection accidentally from inhalation of virus-contaminated aerosols of rodent excreta. Hantavirus genotypes may be nonpathogenic for humans or cause serious diseases with high death rates. In Eurasia, these pathogens involve primarily the kidney and cause hemorrhagic fever with renal syndrome; in North and South America, these pathogens involve primarily the lung and cause hantavirus cardiopulmonary syndrome.

First reports on the occurrence of hantaviruses in central Europe originated from former Czechoslovakia (2,3) and Germany (4) and date back to 1984 and 1985, respectively. The first cases of hantavirus disease in humans in the Czech Republic were reported in 1992 (5). This flulike disease accompanied by microhematuria was recorded in southern Moravia. Another severe imported case was described in a soldier on active military duty in the Balkans (6). The first isolation of nonpathogenic hantavirus Tula was reported in the Czech Republic (7). Currently, several hantavirus infections have been recorded in humans, manifesting mainly as interstitial nephritis. One fatal case was also reported in a patient who had never travelled outside the Czech Republic.

We conducted studies of hantavirus ecology in the Czech Republic and hantavirus seroprevalence in the Czech population. As in neighboring Slovakia (8), hantaviruses of three genotypes, i.e., Dobrava, Puumala, and Tula, were identified in the Czech Republic. Most serious infections are caused by the Dobrava genotype; Tula genotype remains nonpathogenic for humans, although a case of human infection without clinical signs has been described in the Czech Republic (9).

Blood serum samples from 710 randomly selected persons >20 years of age from the Czech Republic were screened for antibodies against Puumala and Hantaan antigens with commercial enzyme-linked immuno-sorbent assay (ELISA) sets manufactured by PROGEN (Biotechnik, Heidelberg, Germany). The Hantaan antigen was used because of its antigenic relatedness with Dobrava virus, which is not included in available commercial ELISA sets.

Five participants showed immuno-globulin (Ig) G reactivity to Hantaan virus (cross-reactive with Dobrava antigen), and two participants tested positive for both IgG and IgM antibodies. Two other persons showed IgM reactivity alone. These findings indicate that as many as seven (1.0%) study participants showed reactivity to Hantaan antigen. Eight persons showed IgG reactivity to Puumala antigen, none of them IgM positive. Altogether, 10 (1.4%) study participants were reactive to Puumala antigen. Three persons showed reactivity to both antigens tested.

A total of 1,494 small mammals of different Czech regions were screened with ELISA for hantavirus antigen in the lungs. The antigen was detected in the lungs of 101 animals; the highest positivity rate was in Common Voles (Microtus arvalis). The difference in positivity between male and female voles was not statistically significant. The positivity rate was markedly associated with rodent size. With the use of molecular genetic methods (polymerase chain reaction), genotype Tula was identified as the causative agent of infection in rodents. Genotype Puumala was identified in bank voles (Clethrionomys glareolus) in Moravia. Nucleotide sequences of Dobrava genotype were identified in southern Bohemian rodents (K. Krivanec, pers. comm.).

In the Czech Republic, Tula virus is the most frequent hantavirus circulating in Common Voles. This agent is not pathogenic for humans. The hantavirus seroprevalence rate in the adult population of the Czech Republic is close to 1%. Dobrava and Puumala viruses are causative agents of these infections in humans.

This research was supported by grants no NI 5896-3/2000 of the Grant Agency of the Ministry of Health of the Czech Republic and no. EVK-CT-2000-00070 of the EC cCASHh.


(1.) Lee HW, Lee PW, Johnson KM. Isolation of the etiologic agent of Korean hemorrhagic fever. J Infect Dis 1978;137: 298-308.

(2.) Gresikova M, Rajcani J, Sekeyova M, Brummer-Korvenkontio M, Kozuch O, Labuda M, et al. Haemorrhagic fever virus with renal syndrome in small rodents in Czechoslovakia. Acta Virol 1984;28 :416-21.

(3.) Danes L. [Hemorrhagic fever with renal syndrome (HFRS)] (in Czech). Acta Hygienica, Epidemiologica et Microbiologica 1985;15:52-7.

(4.) Pilaski J, Zoller L, Blenk H. Hamorrhagisches Fieber mit renalem Syndrm (HFRS): eine durch Nagetiere ubertragene Nephropathie des Menschen. Wehrmedizinische Monatschrift 1986; 10:435-44.

(5.) Kobzik J, Danes L. [Laboratory-confirmed cases of hemorrhagic fever with renal syndrome which occurred in Breclav 1989-1990] (in Czech). Ceskoslovenska Epidemiologie, Mikrobiologie a Imunologie 1992;41:65-8.

(6.) Petru K, Pejcoch M, Monhart V, Matyasov aI. [Hemorrhagic fever with renal syndrome] (in Czech) Cas Lek Cesk 1997;136:739-40.

(7.) Plyusnin A, Cheng Y, Vapalahti O, Pejcoch M, Unar J, Jelinkova Z, et al. Genetic variation in Tula hantvariuses: sequence analysis of the S and M segments of strains from central Europe. Virus Res 1995;39:237-50.

(8.) Sibold C, Meisel H, Lundkvist, Schulz A, Cifire F, Ulrich R, et al. Short report: simultaneous occurrence of Dobrava, Puumala, and Tula hantaviruses in Slovakia. Am J Trop Med Hyg 1999;61:409-11.

(9.) Vapalahti O, Lundkvist A, Kukkonen SK, Cheng Y, Gilljam M, Kanerva M, et al. Isolation and characterization of Tula virus, a distinct serotype in the genus Hantavirus, family Bunyaviridae. J Gen Virol 1996;77:3063-7.

Address for correspondence: Milan Pejcoch, Health Institute, Gorkeho 6, 602 00 Brno, Czech Republic; fax: +420 541 213 548; email:

Milan Pejcoch * and Bohumir Kriz *

* National Institute of Public Health, Prague, Czech Republic
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Author:Kriz, Bohumir
Publication:Emerging Infectious Diseases
Article Type:Letter to the Editor
Geographic Code:4EXCZ
Date:Jun 1, 2003
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