Printer Friendly

Glowing evidence of gene-altered arteries.

One of summer's delights is the pulsing glow of hundreds of fireflies dotting the dusky sky. New research suggests that this same power source can help light the way to a gene therapy for coronary artery disease.

A 1989 report of the first successful transfer of a bacterial gene into the leg arteries of live animals (SN: 6/17/89, p.373) took molecular biologists one step closer to the prospect of a gene therapy for humans who suffer from clogged arteries. Researchers have now added a luminous twist to such experiments, this time inserting dog arteries with the gene coding for luciferase -- the enzyme that gives the firefly its "fire."

In one experiment, Judith L. Swain and her colleagues at Duke University Medical Center in Durham, N.C., anesthetized seven dogs and surgically exposed the femoral arteries of the legs. They then inserted catheters and flushed the arteries with a solution containing luciferase genes. The enzyme's glow, they reasoned, would signify gene activation. After three days, they removed the tissue and exposed it to a light-measuring device.

In six of the animals, the glow-meter readings indicated that the altered femoral tissue contained 1 to 77 picograms of luciferase, averaging about 20 picograms. This showed that the gene had traveled through the cell membrane and "turned on," directing the canine cell to secrete the firefly enzyme, Swain's group reports in the June CIRCULATION. In a separate experiment, the team transferred the luciferase gene into the coronary arteries of two dogs, later detecting 28 and 32 picograms of the enzyme.

While no one proposes injecting firefly genes into people, the study does brighten hopes that scientists may someday inject human coronary arteries with genes that code for powerful clot-dissolving proteins such as tissue plasminogen activator (TPA), Swain says. The inserted genes might turn artery cells into miniature TPA factories that churn out enough of the drug to prevent the blood clots that can trigger a heart attack, she adds.

People undergoing angioplasty, in which doctors use a tiny balloon to open clogged arteries, might be prime candidates for such gene therapy, Swain suggests. Although physicians currently give angioplasty patients intravenous injections of TPA, this doesn't always prevent the arteries from later reclogging or developing clots. Swain cautions, however, that a gene therapy to keep human arteries open will take many more years to develop.
COPYRIGHT 1991 Science Service, Inc.
No portion of this article can be reproduced without the express written permission from the copyright holder.
Copyright 1991, Gale Group. All rights reserved. Gale Group is a Thomson Corporation Company.

Article Details
Printer friendly Cite/link Email Feedback
Title Annotation:using luciferase enzyme from fireflies to in gene therapy for coronary artery disease
Author:Fackelmann, Kathy A.
Publication:Science News
Date:Jun 22, 1991
Previous Article:Camouflaged grafts elude immune detection.
Next Article:Pigs make human pigment.

Related Articles
Young hearts: researchers try new ways to prevent tomorrow's heart attacks.
Beetlejuice genes now in biotechnicolor.
Now in vivo: altering endothelial cells.
First human gene-therapy test begun.
Human gene therapy wins crucial victory.
A heartening finding for women on aspirin.
Stress puts squeeze on clogged vessels.
'Good' lipoprotein shows its bad side.
Gene therapy takes on cholesterol defect.

Terms of use | Copyright © 2016 Farlex, Inc. | Feedback | For webmasters