Get a grip. (Junior Division).
The purpose of this project is to culture a variety of bacteria on
a number of different door handles. The quantity and kinds of bacteria
found on them will be determined using nutrient agar and Mac Conkey
Agar, and stained using gram positive and safranin stains. I hypothesize that there will be more bacteria on the toilet handles then on any other
handles and that the door handles going into the bathrooms will have
more bacteria then the door handles going out of the bathrooms. The
handles from the twelve locations were swabbed using nutrient agar and
Mac Conkey agar. The petri dishes were placed in the incubator. The
bacteria were observed. The bacteria off the nutrient agar were
transferred onto slides to be observed. The slides were stained with
crystal violet, safranin, and gram iodine, and the bacteria were
observed under a composite light microscope. When analyzing the nutrient
agar test and Mac Conkey media combined, the toilet handles' from
all of the locations had the largest number of colonies for that
location. The inside door handle's came in second, having the
second largest number of bacteria colonies overall.
Darrick R. Ohr, Woodlin School.