Geographical color pattern of Argia apicalis (Odonata: Coenagrionidae) in the absence of molecular variation.
Argia apicalis is an ecologically vagile species inhabiting both pond and stream environments. Say (1839) diagnosed A. apicalis as having a pearlaceous blue thoracic region and a hairline humeral and thin dorsal stripe (Fig. 1A). Other characters used to identify A. apicalis are the distinctive pointed and tooth-like cercus in males (Fig. 1B) and a distribution east of the Rocky Mountains and into Arizona (Fig. 1C) (Garrison 1994).
Bick & Bick (1965), Dunkle (1990), Johnson & Westfall (1970), and Johnson (1972) noted variation of the humeral stripe in southeastern populations of A. apicalis. The subpopulation of A. apicalis in the southeastern range (Suwannee County and Columbia County, Florida) have a broad humeral stripe across the length of the pterothorax. In his analysis of the geographical variation within A. apicalis in Florida, Johnson (1972) categorized both males and females into groupings based on the amount of black markings on the head, thorax, and abdomen, and defined "typical" A. apicalis as having humeral stripe values of 1 or 2, whereas "atypical" specimens had values of 4 or 5. Specimens with a value of 3 were deemed intermediates. Johnson (1972) then discussed the pattern variability in the southeastern distribution and noted that in this region specimens with the typical pattern were less than 5% and specimens with atypical thoracic patterns represented more than 95% of the population. In the summer of 2013, 2 authors herein (Smith-Herron & T. Cook) collected A. apicalis near the Suwannee River and noted that 100% of their specimens (122 specimens) fit Johnson's definition of atypical. This study is the first to combine color pattern and cytochrome-b gene sequences to document variation within an extensive portion of the distribution range of A. apicalis.
Materials and Methods
Argia apicalis adults were collected using aerial nets in May through Sep 2013 and 2014 from 10 localities in Texas, Louisiana, Florida, and Oklahoma (Table 1). Individuals were field preserved in 70 to 90% ethanol and subsequently dried and curated upon arrival at the laboratory. Specimens were photographed with a Canon EOS 70D mounted on an Olympus SZX12 microscope.
We also obtained about 200 specimens on loan from the following museum collections: National Museum of Natural History (NMNH), International Odonata Research Institute (IORI), Georgia Museum of Natural History (GMNH), and Sam Houston State Entomology Collection (SHSUEC), which allowed us to augment our coverage of the distributional range of A. apicalis. The combination of our field-collected samples with existing museum specimens represent about 59% of the reported distribution of A. apicalis in the United States (Fig. 2) (Westfall & May 2006).
Since its description in 1839, researchers have used hairline humeral stripe found on the thorax to identify and distinguish A. apicalis from its congeners (Johnson 1972). We examined the width of the humeral stripe of the thorax and the pattern and coloration of the head and thorax. For the humeral stripe, the width of the stripe, along the entire length of the thorax, was evaluated and whether or how much it narrowed along its length (Fig. 3). Johnson (1972) reported that individuals with large black patterning on the head usually had hairline humeral stripes whereas individuals with small patterning had wider humeral stripes. To test this observation, we examined head and abdomen of specimens for distinct patterns between the southeastern and the northwestern populations. We also evaluated variation in the morphology of the male caudal appendages and female mesostigmal plates (Garrison 1994; Westfall & May 2006).
We extracted genomic DNA from legs for a subset of 29 individuals (Table 2) following the standard protocol instructions of Zymo Research's Quick-gDNA[TM] Mini Prep extraction kit (Zymo Research, Irvine, California). Individuals selected for DNA extraction were taken from field-collected specimens as most museum specimens included in the morphological analyses were collected over 20 yr ago. Special care was taken to select individuals from populations throughout the geographic distribution of A. apicalis (i.e., Florida, Kansas, Louisiana, Oklahoma, and Texas). Although the mitochondrial cytochrome oxidase I (COI) gene has been used widely to detect cryptic diversity and inter-population differentiation in odonates (Brown et al. 2000) and other hexapods (Folmer et al. 1994; Marcus et al. 2009), repeated attempts to amplify this gene fragment in our samples using various previously published primers [e.g. HCO/LCO (Folmer et al. 1994), EVA/ JERRY (Blum et al. 2003)] failed to produce positive amplicons. We thus decided to PCR amplify a 361 bp fragment of the mitochondrial gene cytochrome-b (cyt-b) using primers (151F: 5'-TGTGGRGCNACYGTW-3'; 270R: 5'-AANAGGAARTAYCAYTCNGGYTG-3') and conditions published by Merritt et al. (1998). The usefulness of this gene fragment in detecting cryptic diversity and population structure in insects and other arthropods is well established (Simmons & Weller 2001; Santamaria et al. 2013). Positive amplicons were cleaned and sequenced at Genewiz (South Plainfield, New Jersey), with resulting sequences assembled and edited (e.g., removal of primer regions) using Geneious R 8.0.2 (Biomatters Ltd.). Dried specimens from which DNA was extracted were labeled as DNA vouchers and deposited into the SHSUEC. DNA template vouchers are stored at -40 [degrees]C in the Sam Houston State Natural History Collections (SHSNHC) housed at the Texas Research Institute for Environmental Studies (TRIES) facility, Sam Houston State University.
ANALYSIS OF COLOR PATTERN
Specimens from the northwestern distribution of A. apicalis (north of the Florida Panhandle) displayed a "typical" hair-line humeral stripe. The humeral stripe in this distribution range varied from almost absent in some specimens to very narrow at most, and rarely extended more than a third of the length of the pterothorax. Although the thickness of the humeral stripe varied in the northwestern populations, the variation was not as pronounced as that between northwestern and southeastern populations (Figs. 4-6). Southeastern populations had an "atypical" humeral stripe that was much wider than the "typical" variation and generally extended beyond the anterior third of the pterothorax and might extend the entire length of the pterothorax. Patterns on the head and abdomen were variable among all individuals and were not correlated with geography. After examining all specimens, we noted that the black patterns on the head and abdomen were variable across all specimens and all geographic regions.
All sequences produced in this study were deposited in GenBank under accession numbers KP770147 to KP770165. We successfully sequenced the target cyt-b gene fragment for 19 A. apicalis individuals from throughout its range in the United States: 8 from 5 populations in Texas, 7 from a single population in Florida, 3 from 2 populations in Oklahoma, and 1 from a single population in Kansas. All 19 individuals harbored a single haplotype for the cyt-b gene, indicating no divergence between individuals, populations, or regions. The remaining 11 DNA extractions (4 from Texas, 1 from Florida, 1 from Oklahoma, 2 from Louisiana, 2 from Kansas, and 1 from Nebraska) failed to produce positive amplicons despite repeated attempts at PCR amplification and re-extraction of DNA. Given our findings of non-existent genetic divergence in this gene, we decided against further efforts to produce sequences for these samples.
The 2 color forms of A. apicalis were described briefly by Johnson (1972) and Dunkle (1990). Descriptions of A. apicalis have characterized it as having a pterothorax with a narrow dorsal stripe and either no or at most thin humeral stripe, except for populations from Florida in which the humeral stripe is wider and extends more than half the length of the pterothorax. The geographical divide of the 2 color forms is the Suwannee River and reflects the population disjunctions of the sub-species of A. fumipennis in which A. f. atra occurs east of the Suwannee River, A. f. fumipennis west of the Suwannee River, and A. f. violacea north/northwest of Florida. Where the color morphs overlap, they are intermediate in the color pattern and cannot be identified further.
The 2 color forms of A. apicalis were once documented to coexist near the Suwannee River but their co-existence may no longer be true. As we found no differences in cyt-b gene sequences between the 2 color morphs throughout their distribution range, their recognition as separate species is not justified. Although no mechanism or cause is known for the variation seen in both the A. fumipennis and A. apicalis complexes, Johnson (1972) suggested allopatric processes associated with sea level changes during the Pleistocene (e.g., fragmentation of Florida into islands and formation of the Suwannee Straits) may be responsible for the observed differences.
The authors would like to thank Karen Pruitt and Cowboy Joe Herron for aiding in the collection of specimens from west and south Texas and Lenora Reid for granting permission to collect on her property. We would also like to extend our appreciation to Daniel Haarmann, Sibyl R. Bucheli, Brent C. Rahlwes, Soheyla Bayat, and Ashley R. Morgan for their assistance in the identification of specimens and discussion of molecular techniques. We are truly grateful to Christopher P. Randle, who allowed the use of his research lab to conduct the molecular portion of this research. The authors would like to acknowledge the Georgia Museum of Natural History, the International Odonata Research Institute, the National Museum of Natural History, and the Sam Houston State University Natural History Collections for providing loan specimens that aided in the overall research. Collection permits were granted by the Florida Department of Environmental Protection Division of Recreation and Parks (#05291310) and the Louisiana Department of Wildlife and Fisheries. This research was made possible by funding provided by a Sam Houston State University Enhancement Research Grant (#290041). Lastly, we would like to thank the several anonymous reviewers for their careful edits and helpful comments that greatly improved the manuscript.
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Melissa S. Sisson (1,2) *, Carlos A. Santamaria (3,4), Autumn J. Smith-Herron (1), Tamara J. Cook (3), and Jerry L. Cook (5)
(1) Texas Invasive Species Institute, Sam Houston State University, Huntsville, Texas 77341, USA; E-mail: firstname.lastname@example.org (M. S. S.), email@example.com (A. J. S.-H.)
(2) University of North Dakota, Department of Biology, Grand Forks, North Dakota 58203, USA
(3) Sam Houston State University, Department of Biological Sciences, Huntsville, Texas 77341, USA; E-mail: firstname.lastname@example.org (C. A. S.), email@example.com (T. J. C.)
(4) Biology Faculty, College of Arts and Sciences, University of South Florida Sarasota-Manatee, Sarasota, Florida 34243, USA
(5) Sam Houston State University, Office of Research and Sponsored Programs, Huntsville, Texas 77341, USA; E-mail: firstname.lastname@example.org (J. L. C.)
* Corresponding author; Email: email@example.com (M. S. S.)
Caption: Fig. 1. The 4 defining characters of Argia apicalis; (A) a pearlaceous blue pterothorax and a hairline humeral stripe; (B) a thin dorsal stripe; (C) males have a distinctive pointed and tooth-like cercus; (D) and their distribution east of the Rocky Mountains (shaded areas on map are the recorded distribution of A. apicalis).
Caption: Fig. 2. (A) Distribution map of specimens examined (this accounts for about 59% of the reported distribution of Argia apicalis). Dark gray states with black dots represent collected specimen localities, and light gray states with no dots represent areas where no specimens were collected. (B) Distribution of color morphs of A. apicalis in Florida; dark gray represents counties with typical A. apicalis, light gray represents counties with atypical A. apicalis, and the striped area represents the county in which both typical and atypical A. apicalis morphs are present.
Caption: Fig. 3. Geographical color variation: individuals from the (A) northwestern range of the distribution have a typical (= narrow) humeral stripe, whereas individuals from the (B) southeastern part of the range have an atypical (= wide) humeral stripe.
Caption: Fig. 4. Variation in width and extension of humeral stripes in the northwest region of the distribution: (A) Dallas County, Texas; (B) Fairfax County, Virginia; (C) Hunterdon County, New Jersey; (D) Iowa; (E) Missouri County, Oregon; (F) Washington Parish, Louisiana; (G) Holmes County, Florida; (H) Wharton County, Texas; and (I) Washington D.C.
Caption: Fig. 5. Identifying characters of Argia apicalis in the southeast: (A) humeral stripe wide extending at least three-quarters of the pterothorax length; (B) middorsal line slightly wider than in northwestern A. apicalis; and (C) paler caudal appendage (whiter) than in individuals from the north.
Caption: Fig. 6. Variation of humeral stripes in the southeast region of the distribution: (A) Clay County, Florida; (B) Columbia County; Florida; (C) Wakulla County, Florida; and (D) Suwannee County, Florida.
Table 1. List of Argia apicalis specimens examined for color pattern analysis. Museum Date Sex State accession numbers USMN 00354813 1 VIII 1914 F DC USMN 00354821 22 VIII 1974 M FL USMN 00354822 22 VIII 1974 F FL IORI 00038262 10 VI 1954 -- FL IORI 15 VIII 1969 M FL IORI 12 IV 1975 F FL IORI 25 VIII 1950 M FL IORI 12 VII 1972 M FL IORI 2 VIII 1969 M FL IORI 11 VIII 1969 M FL IORI 28 V1973 M FL IORI 28 V1973 F FL IORI 22 VIII 1984 F FL IORI 12 VIII 1969 M FL IORI 7 V 1981 -- FL IORI 33 VIII 1984 M FL IORI 12 VII 1972 F FL IORI 29 IV 1973 M FL IORI 18 IX 1971 F FL USMN 00354826 20 VIII 1946 M/F GA GMNH 10 VII 1955 -- GA GMNH 7 VII 1971 -- GA GMNH 23 VI 1950 -- GA GMNH 20 V1984 -- GA GMNH 13 VI 1950 -- GA GMNH 22-27 VII 1970 -- GA GMNH 20 VI 1938 -- GA GMNH 20 VI 1937 -- GA GMNH 26V1953 -- GA GMNH 9-13 V 1994 -- GA GMNH VI 1937 -- GA GMNH 2 VII 1944 -- GA GMNH 27 VII 1944 -- GA GMNH 2 VII 1944 -- GA GMNH 25 VII 1941 -- GA GMNH 24 VII 1931 -- GA GMNH 16 VI 1946 -- GA USMN 00354841 24 VII 1929 M IL USMN 00354842 24 VII 1929 F IL USMN 00355162 26 VII 1953 M IN USMN 00355164 26 VII 1953 M IN USMN 00354873 8 VII 1927 M IA USMN 00354875 15 VII 1927 F IA USMN 00354900 10 VII 1943 M KS USMN 00354901 10 VII 1943 F KS USMN 00354908 18 VI 1947 F KY USMN 00354906 29 VII 1948 M KY USMN 00354909 12 VIII 1925 F LA USMN 00354910 12 VIII 1925 M LA USMN 00354930 21-28 VII 1903 M MD USMN 00354928 21-28 VII 1903 F MD USMN 00354969 16 VII 1907 M MN USMN 00354970 16 VII 1907 F MN USMN 00354981 28 VIII 1949 F MO USMN 00354979 28 VIII 1949 M MO GMNH 16 IX 1972 -- MO USMN 00355192 20 VII 1998 M NB USMN 00355192 20 VII 1998 M NB USMN 00355200 3 VIII 1985 F NJ USMN 00355204 3 VIII 1985 M NJ USMN 00355209 19 IX 1988 M NM USMN 00355213 18 VIII 1939 F NC USMN 00355214 18 VIII 1939 M NC USMN 00355218 20 VII 1926 F OH USMN 00355222 20 VII 1926 M OH USMN 00355247 1962 F OK USMN 00355248 1960 M Ok USMN 00355254 13-14 VI 1970 M PA USMN 00355257 18 VII 1939 M SC USMN 00355256 18 VII 1939 F SC USMN 00355275 14 VIII 1939 F TN USMN 00355272 31 V 1953 M TN USMN 00355297 7 IX 1949 M TX USMN 00355303 15-17 VI 1965 M TX USMN 00355304 8 VI 1965 F TX USMN 00355309 26 VI 1904 M/F TX USMN 00355306 15-17 VI 1965 M TX USMN 00391416 12-26 VII 2007 F VA USMN 00391417 12-26 VII 2007 M VA USMN 00713680 13 VI 2007 F WV USMN 00713676 24 VIII 2004 M WV SHSUE 026337 20 VII 2013 M FL SHSUE 026336 20 VII 2013 M FL SHSUE 026339 20 VII 2013 F FL SHSUE 026338 20 VII 2013 M FL SHSUE 026335 20 VII 2013 F FL SHSUE 026344 20 VII 2013 F FL SHSUE 026345 20 VII 2013 M FL SHSUE 026349 31 VIII 2013 F TX SHSUE 026348 31 VIII 2013 F TX SHSUE 026346 31 VIII 2013 M TX SHSUE 000185 1 IX 2007 -- OK SHSUE 000161 23 VII 2007 -- TX SHSUE 000189 10 VIII 2007 -- TX SHSUE 001293 25 VII 2012 -- TX SHSUE 000173 1 VII 2007 -- NE SHSUE 026343 20 VII 2013 M FL SHSUE 026340 19 IX 2013 M OK SHSUE 000195 2 IX 2007 -- KS SHSUE 000162 2 VII 2007 -- KS SHSUE 008488 12 VI 2013 -- LA SHSUE 008519 17 VI 2013 -- LA SHSUE 000692 1 VII 2011 -- TX SHSUE 026347 31 VIII 2013 F TX SHSUE 026341 19 IX 2013 F OK SHSUE 026342 19 IX 2013 F OK SHSUE 008592 20 VII 2013 -- FL SHSUE 008599 20 VII 2013 -- FL SHSUE 008601 20 VII 2013 -- FL SHSUE 008600 20 VII 2013 -- FL SHSUE 008602 20 VII 2013 -- FL SHSUE 008603 20 VII 2013 -- FL SHSUE 008604 20 VII 2013 -- FL SHSUE 008579 20 VII 2013 -- FL SHSUE 008580 20 VII 2013 -- FL SHSUE 008581 20 VII 2013 -- FL SHSUE 008582 20 VII 2013 -- FL SHSUE 008583 20 VII 2013 -- FL SHSUE 008585 20 VII 2013 -- FL SHSUE 008586 20 VII 2013 -- FL SHSUE 014225 24 VII 2013 -- TX SHSUE 014226 24 VII 2013 -- TX SHSUE 026437 20 V 2014 -- TX SHSUE 026438 20 V 2014 -- TX SHSUE 007459 20 V 2014 -- TX SHSUE 007460 20 V 2014 -- TX SHSUE 007461 20 V 2014 -- TX SHSUE 007462 20 V 2014 -- TX Museum County/Parish Locality accession numbers USMN 00354813 N/A C&O canal, Chain Bridge USMN 00354821 Jackson N/A USMN 00354822 Jackson N/A IORI 00038262 Liberty Torreya State Park IORI Holmes Choctowhatchee River at US 90 IORI n/a Escambia River IORI Columbia Santa Fe River IORI Clay Black Creek, N. Prong, Hwy 209 IORI Suwannee Suwannee River, Suwannee State Park IORI Wakulla St. Marks River at Newport on US 98 IORI Gadsden Near Apalachicola River, Hwy 90, Chattahoochee IORI Gadsden Near Apalachicola River, Hwy 90, Chattahoochee IORI Jackson Three Rivers State Park IORI Liberty Ochlockonee River at Hwy 20 IORI Washington Choctowhatchee River at US 90 IORI Jackson Three Rivers State Park IORI Clay Black Creek, N. Prong , Hwy 209 IORI Columbia Pond near Santa Fe River, Hwy 441 IORI Alachua Santa Fe River, near Hwy 235 USMN 00354826 Decatur Spring Creek (mating pair) GMNH Morgan N/A GMNH Clarke N/A GMNH Sumter N/A GMNH Clarke N/A GMNH Sumter N/A GMNH Clarke N/A GMNH Clarke N/A GMNH Clarke N/A GMNH Clarke N/A GMNH Clarke 1.1 mile SW of Winterville GMNH Clarke N/A GMNH Fulton Bolton GMNH Fulton/Dekalb Atlanta GMNH Fulton Bolton GMNH Putnam Eatonton GMNH Whitfield Dalton GMNH Decatur Spring Creek USMN 00354841 Lake 4-5 mi. N. of Olney USMN 00354842 Lake 4-5 mi. N. of Olney USMN 00355162 Tippecanoe Wabash River, N. of Lafayette USMN 00355164 Tippecanoe Wabash River, N. of Lafayette USMN 00354873 N/A Iowa River; SUI Campus USMN 00354875 N/A Iowa River; SUI Campus USMN 00354900 Sumner Caldwell USMN 00354901 Sumner Caldwell USMN 00354908 Green Crailhope; Little Barren River USMN 00354906 Green Crailhope; Little Barren River USMN 00354909 Madison Eagle Lake USMN 00354910 Madison Eagle Lake USMN 00354930 Montgomery Barnesville; C&O canal USMN 00354928 Montgomery Barnesville; C&O canal USMN 00354969 Washington Stillwater USMN 00354970 Washington Stillwater USMN 00354981 Oregon N/A USMN 00354979 Oregon N/A GMNH Boone N/A USMN 00355192 Cherry Along Niobrara R; Allen Bridge; 4 mi. S of Sparks USMN 00355192 Cherry Along Niobrara R; Allen Bridge; 4 mi. S of Sparks USMN 00355200 Hunterdon Lockatong Cr. On Hwy 29 N; Stockton USMN 00355204 Hunterdon Lockatong Cr. On Hwy 29 N; Stockton USMN 00355209 Guadalupe Santa Rosa USMN 00355213 Swain/Jackson Cherokee; Hiwassee River; Murphy USMN 00355214 Swain/Jackson Cherokee; Hiwassee River; Murphy USMN 00355218 Erie Huron R. ; 5 mi. S. of Huron USMN 00355222 Erie Huron R. ; 5 mi. S. of Huron USMN 00355247 Paine N/A USMN 00355248 Garfield N/A USMN 00355254 York Conewago Cr.; 5 mi. NW of Davidsburg USMN 00355257 Greenville Lakeside; a lake approx. 7 mi. S. of Greenville USMN 00355256 Greenville Lakeside; a lake approx. 7 mi. S. of Greenville USMN 00355275 Campbell Lafollette; Cove Lake USMN 00355272 Davidson Nashville; pool in Centennial Park USMN 00355297 Cameron Brownsville USMN 00355303 San Jacinto Near Coldspring USMN 00355304 Wharton El Campo USMN 00355309 n/a Dallas (mating pair) USMN 00355306 San Jacinto Near Coldspring USMN 00391416 Fairfax Quarry; Great Falls; 39.984722 -77.250278 USMN 00391417 Fairfax Swamp Trail; Great Falls; 38.984444 -77..250556 USMN 00713680 Marshall Dunkard; Fork Lake; 1.2 mi. S. of Majorsville off CR 15 USMN 00713676 Harrison Good Hope; West Fork River SHSUE 026337 Suwannee Suwannee State Park along the river SHSUE 026336 Suwannee Suwannee State Park along the river SHSUE 026339 Suwannee Suwannee State Park along the river SHSUE 026338 Suwannee Suwannee State Park along the river SHSUE 026335 Suwannee Suwannee State Park along the river SHSUE 026344 Suwannee Suwannee State Park along the river SHSUE 026345 Suwannee Suwannee State Park along the river SHSUE 026349 Taylor Jim Ned Creek SHSUE 026348 Taylor Jim Ned Creek SHSUE 026346 Taylor Jim Ned Creek SHSUE 000185 Tulsa Mohawk State Park SHSUE 000161 Brown Yegua Creek SHSUE 000189 Parker Mineral Wells SHSUE 001293 Walker Raspberry Pond, Phelps, TX SHSUE 000173 Omaha Easley Creek SHSUE 026343 Suwannee Jim Ned Creek SHSUE 026340 Waynoka Waynoka Stream SHSUE 000195 Sedewick Pawnee Prairie SHSUE 000162 Brown Delaware River SHSUE 008488 Calcacien Sam Houston Jones State Park SHSUE 008519 Washington Bogue Chitto Stream SHSUE 000692 Taylor Jim Ned Creek SHSUE 026347 Taylor Jim Ned Creek SHSUE 026341 Waynoka Waynoka Stream SHSUE 026342 Waynoka Waynoka Stream SHSUE 008592 Suwannee Suwannee State River Park SHSUE 008599 Suwannee Suwannee State River Park SHSUE 008601 Suwannee Suwannee State River Park SHSUE 008600 Suwannee Suwannee State River Park SHSUE 008602 Suwannee Suwannee State River Park SHSUE 008603 Suwannee Suwannee State River Park SHSUE 008604 Suwannee Suwannee State River Park SHSUE 008579 Suwannee Suwannee State River Park SHSUE 008580 Suwannee Suwannee State River Park SHSUE 008581 Suwannee Suwannee State River Park SHSUE 008582 Suwannee Suwannee State River Park SHSUE 008583 Suwannee Suwannee State River Park SHSUE 008585 Suwannee Suwannee State River Park SHSUE 008586 Suwannee Suwannee State River Park SHSUE 014225 Liberty Big Thicket: Birdwatcher's Trail SHSUE 014226 Liberty Big Thicket: Birdwatcher's Trail SHSUE 026437 Brewster Rio Grande River SHSUE 026438 Brewster Rio Grande River SHSUE 007459 Brewster Rio Grande River SHSUE 007460 Brewster Rio Grande River SHSUE 007461 Brewster Rio Grande River SHSUE 007462 Brewster Rio Grande River Table 2. List of Argia apicalis specimens processed for molecular analysis. Museum Date Sex State accession numbers SHSUE 026337 20 VII 2013 M FL SHSUE 026336 20 VII 2013 M FL SHSUE 026339 20 VII 2013 F FL SHSUE 026338 20 VII 2013 M FL SHSUE 026335 20 VII 2013 F FL SHSUE 026344 20 VII 2013 F FL SHSUE 026345 20 VII 2013 M FL SHSUE 026349 31 VIII 2013 F TX SHSUE 026348 31 VIII 2013 F TX SHSUE 026346 31 VIII 2013 M TX SHSUE 000185 1 IX 2007 -- OK SHSUE 000161 23 VII 2007 -- TX SHSUE 000189 10 VIII 2007 -- TX SHSUE 001293 25 VII 2012 -- TX SHSUE 000173 1 VII 2007 -- NE SHSUE 026343 20 VII 2013 M FL SHSUE 026340 19 IX 2013 M OK SHSUE 000195 2 IX 2007 -- KS SHSUE 000162 2 VII 2007 -- KS SHSUE 008488 12 VI 2013 -- LA SHSUE 008519 17 VI 2013 -- LA SHSUE 000692 1 VII 2011 -- TX SHSUE 026347 31 VIII 2013 F TX SHSUE 026341 19 IX 2013 F OK SHSUE 026342 19 IX 2013 F OK SHSUE 007459 20V2014 -- TX SHSUE 007460 20V2014 -- TX SHSUE 007461 20V2014 -- TX SHSUE 007462 20V2014 -- TX Museum County/Parish Locality accession numbers SHSUE 026337 Suwannee Suwannee State River Park SHSUE 026336 Suwannee Suwannee State River Park SHSUE 026339 Suwannee Suwannee State River Park SHSUE 026338 Suwannee Suwannee State River Park SHSUE 026335 Suwannee Suwannee State River Park SHSUE 026344 Suwannee Suwannee State River Park SHSUE 026345 Suwannee Suwannee State River Park SHSUE 026349 Taylor Jim Ned Creek SHSUE 026348 Taylor Jim Ned Creek SHSUE 026346 Taylor Jim Ned Creek SHSUE 000185 Tulsa Mohawk State Park SHSUE 000161 Brown Yegua Creek SHSUE 000189 Parker Mineral Wells SHSUE 001293 Walker Raspberry Pond, Phelps, TX SHSUE 000173 Omaha Easley Creek SHSUE 026343 Suwannee Jim Ned Creek SHSUE 026340 Waynoka Waynoka Stream SHSUE 000195 Sedewick Pawnee Prairie SHSUE 000162 Brown Delaware River SHSUE 008488 Calcacien Sam Houston Jones State Park SHSUE 008519 Washington Bogue Chitto Stream SHSUE 000692 Taylor Jim Ned Creek SHSUE 026347 Taylor Jim Ned Creek SHSUE 026341 Waynoka Waynoka Stream SHSUE 026342 Waynoka Waynoka Stream SHSUE 007459 Brewster Rio Grande River SHSUE 007460 Brewster Rio Grande River SHSUE 007461 Brewster Rio Grande River SHSUE 007462 Brewster Rio Grande River
Please note: Some tables or figures were omitted from this article.
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|Author:||Sisson, Melissa S.; Santamaria, Carlos A.; Smith-Herron, Autumn J.; Cook, Tamara J.; Cook, Jerry L.|
|Date:||Sep 1, 2016|
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