Genomic Analysis of Chino del Tomate Virus Using Agroinfection. (Botany & Plant Ecology).
The new world bipartite Chino del Tomate virus (CdTV) has proven to be a great agricultural problem throughout the southern region of the United States affecting many dicoryledonous plants. CdTV requires an A and a B genome for replication and viral transport, respectively. We have cloned different genomic constructs of the aforementioned genomes within pBS-BS plasmid and infected Arabidopsis thaliana, Nicotiana benthamiana, and Lycopersicon esculentum (Rutgers) plants. By utilizing an A Agrobacterium urn vector (C58 or LBA4404), we are capable of super-infecting the plants in vitro by exposing their leaves to the transformed vector. Our analysis has focused on two different A genomes: the 5L:4 and DBE. Both A genomes were recovered from tomatoes infected with and demonstrating viral symptoms of CdTV. The 5L:4 contains 1.5 genome equivalents and one common region loop, whereas the DBE contains two genome equivalents and two common region loops. DBE contains a single base pair change in the DNA replicase gene compared to 5L:4. Our hypothesis predicts that the 5L:4 will autonomously replicate in plant cells, while the DBE will replicate poorly, if at all. By studying both the A and eventually the B genomes of this virus, we hope to create an RNA antisense system against the viral transport proteins to prevent virus proliferation throughout the plant.
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|Article Type:||Brief Article|
|Date:||Mar 22, 2002|
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