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Genetic parameters in agronomic characters for selection of superior lineages of Ricinus communis L/Parametros geneticos em caracteres agronomicos na selecao de linhagens superiores de Ricinus communis L.

INTRODUCTION

Castor bean plant (Ricinus communis L.) is cultivated in small and medium farms, and in large plantations. It is a non-edible oilseed plant with high oil production capacity, with more than 700 industrial uses, such as the manufacture of paints, varnishes, synthetic fibers, lubricants, solvents, nylon, hydraulic fluids, special greases, cosmetics, and prostheses, and is the only vegetable oil soluble in alcohol (COSTA et al., 2005; BAJAY et al., 2009; JEONG AND PARK, 2009; FALASCA et al., 2012).

To meet the growing need for seeds, genetic improvement of the crop and the creation of new genotypes is the most economical way to achieve the required increase in production of this oilseed (OLIVEIRA JUNIOR & ZANOTTO, 2008; DAPKE et al., 2016; SALIHU et al., 2017). The great variability among castor bean genotypes means there is a large genetic base for the creation of new strains with favorable genetic combinations. Therefore, a substantial challenge for breeders is to aggregate the highest possible frequency of desirable alleles in a single genotype (SEVERINO et al., 2006; PASSOS et al., 2010; PATEL & PATEL, 2014; SILVA et al., 2017).

Genetic improvement depends on the right choice of the best individuals to be the progenitors of the next generations. Thus, the evaluation of genetic parameters, such as coefficient of genetic variance and heritability, are important tools for genetic improvement, since they provide information on genetic variability, the degree of expression of a character between generations, and the possibility of gains through direct or indirect selection (ROCHA et al., 2003; PASSOS et al., 2010). Although, some studies investigating genetic parameters have been carried out on castor bean plants (OLIVEIRA & ZANOTTO, 2008; PASSOS et al., 2010; DA SILVA et al., 2011; OLIVEIRA et al., 2013;), further studies are needed to obtain improved materials.

Estimation of genetic parameters through restricted maximum likelihood/best linear unbiased prediction (REML/BLUP) ensures greater precision and allows inferences to be made about the genetic effects of treatments, allowing a more accurate estimation of the genetic value and a more adequate ranking of the superior individuals, resulting in better selection. However, other models can be applied, especially those that aim for the joint analysis of several experiments (MARTINEZ et al., 2011).

Knowledge on the correlations between agronomic traits is important for genetic improvement studies, since they guide the selection of superior lineages, especially when the characters are favorably correlated, which makes it possible to obtain gains for one character indirectly by selection of an associated character. This is advantageous, especially when a character of high economic value has low heritability or is difficult to evaluate compared with another associated character (CRUZ et al., 1997; RODRIGUES et al., 2010). However, if a character is positively correlated with other characters and negatively correlated with others, it is important to take care to avoid selecting for a character that may cause some undesirable change.

In view of the above, the objective of this study was to evaluate the genetic parameters of agronomic characters in castor bean plant lineages and to quantify the genetic correlations between them.

MATERIALS AND METHODS

The experiment was carried out in two agricultural years, 2010 and 2011, in the experimental area of the Genetic Improvement and Biotechnology Nucleus (NBIO) of the Center for Agrarian, Environmental, and Biological Sciences of the Universidade Federal do Reconcavo da Bahia (CCAAB/UFRB), located in the municipality of Cruz das Almas-Bahia, in a randomized complete block design with four replicates.

The following characteristics were evaluated: seed oil content; vegetative cycle; plant height; and number and weight of seeds per plant in 47 lineages. The study population included individuals derived from selection at 21% selection pressure in a generation with high homozygosity, which was conducted by the single seed descent (SSD) method. The segregating population F2 was obtained by self-fertilization of the F1 population resulting from hybridization of the parents: BRS 149 Nordestina, BRS 188 Paraguacu, EBDA MPA-17, Mirante 10, and Sipeal 28.

The area in which the experiment was installed was properly prepared by plowing and harrowing. After soil analysis, acidity was corrected and the planting hole was fertilized with a dose of 20kg [ha.sup.-1] of N, 80kg [ha.sup.-1] of P, and 40kg [ha.sup.-1] of K. Commercial spacings of m between rows and 1m between plants were used. Planting was performed by direct seeding in the field, using three seeds per hole. Approximately 30 days after planting, manual thinning was performed, leaving only the most vigorous plant in each hole.

To avoid seed loss with the dehiscence of some fruits, the racemes were covered using TNT bags, and harvested when 70% of fruits were dried. In order to complete fruit drying, the racemes were placed in a well-ventilated spot under cover. Seeds that were not removed from the fruits by dehiscence were manually removed with pruning shears.

Vegetative cycle was calculated by determining the difference between the date of germination and the date of flowering The first, third, and fourth racemes of each plant were used to evaluate the number and weight of seeds per plant,. A precision digital scale was used to determine the weight of seeds per plant.

Seed oil content was determined by the low field nuclear magnetic resonance (NMR) system, MQA Oxford 7005 (Oxford Instruments, Abingdon/ Oxfordshire, England), using an electromagnet of 0.47T.

Genetic parameters were estimated based on mixed models of the individual REML/BLUP type using the software R following the model: y=Xr+Zg+Wb+e, where y is the vector of the data, r is the vector of the effects of repetition (assumed as fixed) summed to the general average, g is the vector of genotype effects (assumed as random), b is the vector of block effects (assumed as random), and e is the vector of errors or residuals (assumed as random). Uppercase letters represent the incidence matrixes for these effects. The mixed models were analyzed by the software R (R DEVELOPMENT CORE TEAM, 2011) using the Imer function of the lme4 package (BATES et al., 2015).

The data were submitted to analysis of variance, Pearson's linear correlation analysis, and the average values were grouped by the Scott-Knott test at the 5% level in the software R.

Variance components were estimated using the formulas provided by REZENDE & DUARTE (2007).

RESULTS AND DISCUSSION

Results of the genetic parameters are shown in table 1. The values obtained for the coefficient of genotype variation varied from 2.90 to 14.66. According to RESENDE (2002), the coefficient of genotype variation, as a percentage of the general average, represent the amount of genetic variation that exists. However, these variations may be a consequence of several factors, such as the methods used to estimate the parameters, species studied, and environmental conditions.

Using the coefficient of genotypic variation (CVg), it is possible to compare the genetic variability of the different characteristics analyzed (COSTA et al., 2006; RIBEIRO et al., 2009). The highest estimates for coefficient of genotypic variation were presented by WSP (14.66%) and NSP (12.33%); these values can be considered of moderate magnitude and indicated that among all the characters studied, these showed greater variability, allowing the achievement of gain by selection.

Coefficient of genotypic variation for oil content in the seed was 2.90, demonstrating low variability for this character. This can be explained by the strong selection pressure, since only 21% of the original population possessed oil content higher than 50%. The very close values, with a lower coefficient of genotypic variation, obtained for vegetative cycle and plant height (6.45 and 6.53) may indicate that these characters are highly correlated; therefore, selecting for one character will indirectly select for the other.

Estimates of coefficients of experimental variation (CVe) ranged from 1.22, for oil content in the seed, to 40.75, for number of seeds. Therefore, there was good precision in obtaining and analyzing the data, considering the number of repetitions used.

The CVg/CVe ratio determined the coefficient of relative variation (CVr). This parameter allowed inferences to be made about the possibilities of achieving improvement in the evaluated population. Exception for OCS (2.37), all characters presented values below 1, VEGC (0.55), WSP (0.45), PHE (0.34), and NSP (0.30). VENCOVSKY (1987) suggested that the closer to 1 the CVg/CVe ratio, the better the prospects for character improvement. RIBEIRO et al. (2009) stated that the higher the CVr, the greater the chance of selecting genotypes with superior performance.

Coefficient of heritability expresses the amount of genetic variation in relation to the total phenotypic variation (FALCONER, 1987; VENCOVSKY, 1987). Broad-sense heritability of individual plots varied widely, with a higher value (0.75) for seed oil content and a lower value for number of seeds (0.08). PATEL & PATEL (2014) evaluated the oil content in a hybrid population of castor oil plants and obtained a broad-sense heritability value of 0.53, a value below that obtained in the present study.

Low heritability observed for seed number indicates that selection for this character should be performed with caution, since the environment strongly influences phenotypic expression. In addition, it is not convenient to carry out the selection in early generations, with the need of advancement in the generations, followed by self-fecundation to obtain greater genetic gain.

Vegetative cycle presented a value of 0.23 for broad-sense heritability. This value is considered low compared to the results obtained by PASSOS et al. (2010). Those authors evaluated the vegetative cycle for crosses that originated the population under study, and reported the following values: 0.64 ('BRS 149 Northeast' x 'EBDA'); 0.38 ('BRS 149 Nordestina' x 'Sipeal 28'), 0.86 ('BRS 149 Nordestina' x 'Mirante 10') and 0.65 ('BRS 188 Paraguacu' x 'EBDA MPA 17'). Similarly, OLIVEIRA (2011) reported a value of 0.66 in a segregating F3 population of castor bean plants.

Accuracy is associated with precision in selection, and is the main element of genetic progress that can be altered to maximize the genetic gain. Accuracy is obtained by the correlation between the genotypic value of the genetic treatment and that estimated or predicted from available information from the experiments (COSTA et al., 2005). Values obtained for this parameter can vary from 0 to 1 and can be classified as: very high (rgg [greater than or equal to] 0.90), high (0.70 [less than or equal to] rgg<0.90), moderate (0.50 [less than or equal to] rgg<0.70), and low (rgg<0.50) (RESENDE, 2002; RESENDE & DUARTE, 2007).

Results obtained for accuracy varied from 0.52 for the number of seeds per plant to 0.97 for oil content, and were classified as: moderate for PHE (rgg=0.56), NSP (rgg=0.52) and WSP (rgg=0.67), high for VEGC (rgg=0.74), and very high for oil content (rgg=0.97). For REZENDE (2002), accuracy values exceeding 0.70 are sufficient to ensure good precision on the genetic value, which can be observed for the vegetative cycle and oil content in the present study. However, an experiment with at least seven replicates is required to obtain experiments with accuracy above 0.90 (RESENDE & DUARTE 2007).

The experiment included four replicates; therefore, the number of replicates may have contributed to the low proportion of ideal selective accuracy in most characters. Thus, it is necessary to evaluate experimental techniques that have not previously been used for castor bean plants in order to improve test efficiency. In the present study, the estimates of genetic parameters obtained for the advanced population will serve as a basis for selection of elite lineages of castor bean plant, in order to obtain genetic gains in crop improvement.

Table 2 shows the correlation between the characters evaluated in castor bean plant genotypes in the 2 years of cultivation. Except for the correlations between PHE x NSP and PHE x OCS, there was a significant correlation at the 5% level among all evaluated characters indicating a high degree of association, but with a low proportion.

Knowledge on the magnitude and direction of genotype correlations between characters is extremely important for the breeder to formulate simultaneous selection strategies in multiple characters. This is advantageous, especially when a character of high economic value has low heritability, or is difficult to evaluate compared to another associated character (RODRIGUES et al., 2010; RODRIGUEZ et al., 1998).

There were significant correlations between vegetative cycle and all characters, which were positive with the PHE and negative with the other characters. Similar results were obtained in studies by OLIVEIRA (2011). These correlations indicated that reducing the vegetative cycle yields genotypes with lower height, higher number and weight of seeds per plant, and higher seed oil content. A positive correlation was also observed between vegetative cycle and plant height in a study by ARIF et al. (2017) who evaluated 63 castor bean accessions in Pakistan.

For plant height there was a significant and positive correlation between the vegetative cycle and weight of seeds. These results indicated that, it is possible to obtain productive lineages in the evaluated population, which are early and of low size, attending to the main objective of the genetic improvement program of the crop.

For seed oil content, there was no significant correlation for plant height only. Moreover, the non-significance of the correlation between two characters indicates that they can be manipulated independently during the selection process; thus, when selecting for one of the characters, the average of the other will not be changed (CRUZ & CARNEIRO, 2003).

CONCLUSION

Genetic progress can be made by selecting the character seed oil content due to its considerable heritability and high accuracy in the studied population.

The characteristic of seed number and weight per plant presented greater variability, which allows us to anticipate considerable genetic gains through selection, maintaining the distinction between elite lineages.

Positive correlations between the vegetative cycle and plant height, associated with negative correlations for number and weight of seeds, and oil content, allow significant gains in crop productivity.

http://dx.doi.org/ 10.1590/0103-8478cr20180547

Received 07.11.18

Approved 12.04.18

Returned by the author 01.16.19

CR-2018-0547.R1

ACKNOWLEDGEMENTS

The authors would like to thank Petrobras and the National Petroleum Agency, the Universidade Federal do Reconcavo da Bahia, the Coordination for the Improvement of Higher Education Personnel, and the Advanced Laboratory of Chemical Technology of Embrapa Cotton.

DECLARATION OF CONFLICT OF INTEREST

The authors declare no conflict of interest. The founding sponsors had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, and in the decision to publish the results.

AUTHORS' CONTRIBUTIONS

The authors contributed equally to the manuscript.

REFERENCES

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Laurenice Araujo dos Santos (1) * (iD) Simone Alves Silva (1) Deoclides Ricardo de Souza (2) Gilmara de Melo Araujo (2) Vanessa de Oliveira Almeida (3) (iD) Claudia Garcia Neves (4)

(1) * Center for Agrarian, Environmental and Biological Sciences, Universidade Federal do Reconcavo da Bahia (UFRB), Av. Rui Barbosa, 710, 44380-000, Cruz das Almas, BA, Brasil. E-mail: lasagro@hotmail.com. 'Corresponding author.

(2) Universidade Federal do Reconcavo da Bahia (UFRB), Cruz das Almas, BA, Brasil.

(3) Faculdade Maria Milza (FAMAM), Governador Mangabeira, BA, Brasil.

(4) Universidade Estadual de Santa Cruz (UESC), Rodovia Ilheus, Itabuna, BA, Brasil.
Table 1--Estimation of genetic parameters obtained via restricted
maximum likelihood and best linear unbiased predictor (REML-BLUP) in
castor bean plant lineages in the Reconcavo da Bahia, UFRB, Cruz das
Almas--BA, 2011.

                                             Evaluated characters

Genetic Parameters                         VEGC     PHE     NSP

Genotypic variance                        24.34    0.01    67.48
Environmental variance between blocks      0.34    0.01    52.52
Residual variance                         78.05    0.13    703.55
Individual phenotypic variation           102.73   0.17    823.56
Broad-sense heritability                   0.23    0.09     0.08
Selection accuracy                         0.74    0.56     0.52
Coefficient of genotypic variation/%       6.45    6.53    12.33
Coefficient of residual variation/%       11.55    19.15   40.75
Coefficient of experimental accuracy/%     5.77    9.57    20.37
Coefficient of relative variation          0.55    0.34     0.30
Variance of the prediction error          10.83    0.01    48.77
Standard deviation of genotypic value      3.29    0.10     6.98
Minimum                                     63     1.25      33
Maximum                                     98     2.96     105
Average                                     76     1.90      65

                                          Evaluated characters

Genetic Parameters                         WSP      OCS

Genotypic variance                        43.11    2.42
Environmental variance between blocks     37.63    0.33
Residual variance                         204.60   0.43
Individual phenotypic variation           285.34   3.19
Broad-sense heritability                   0.15    0.75
Selection accuracy                         0.67    0.97
Coefficient of genotypic variation/%      14.66    2.90
Coefficient of residual variation/%       31.94    1.22
Coefficient of experimental accuracy/%    15.97    0.61
Coefficient of relative variation          0.45    2.37
Variance of the prediction error          23.39    0.01
Standard deviation of genotypic value      4.83    0.10
Minimum                                   24.00    50.33
Maximum                                   69.00    57.66
Average                                   44.77    53.63

VEGC--flowering of primary raceme; PHE--plant height; NSP--number of
seeds per plant; WSP--weight of seeds per plant; OCS--oil content in
the seed.

Table 2--Genetic correlations among five characters evaluated
in castor bean plant lineages in the Reconcavo da
Bahia (UFRB), Cruz das Almas--BA, 2011.

        VEGC      PHE      NSP      WSP     OCS

VEGC
PHE    0.08 *
NSP    -0.16 *   0.02m
WSP    -0.15 *   0.08 *   0.76 *
OCS    -0.17 *   -0.02m   0.22 *   0.15 *

Significant at the 5% level (*) and not significant at the 5%
level (NS), by the t-test. Vegetative cycle in days (VEGC);
plant height in m (PHE); number of seeds per plant (NSP);
weight of seeds per plant in g (WSP); percentage of oil content
in the seeds (OCS).
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Title Annotation:BIOLOGY
Author:dos Santos, Laurenice Araujo; Silva, Simone Alves; de Souza, Deoclides Ricardo; Araujo, Gilmara de M
Publication:Ciencia Rural
Date:Jan 1, 2019
Words:3920
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