GENETIC RESISTANCE IN CHICKPEA AGAINST ASCOCHYTA BLIGHT: HISTORICAL EFFORTS AND RECENT ACCOMPLISHMENTS.
Chickpea Blight is a devastating disease of chickpea (Cicer areitinum L.) worldwide caused by Ascochyta rabiei (Pass.) Lab. The disease is more disastrous particularly in long cool and humid environmental spells. It results in huge losses by wiping off all the crop in the desert areas whenever hit its epidemics. To manage this disease, different management strategies are practiced. However, breeding resistance to the host is the best and environmentally safe strategy. During 1970s, the loss of host resistance against the pathogen was reported, so extra ordinary efforts were started by the scientists to enhance the host tolerance towards the pathogen. In this way, relatively simple field screening techniques were followed for breeding and identification of new resistant genotypes.
The review sums up the efforts regarding host breeding against chickpea blight involving large scale field screening experiments as well as recent marker assisted breeding using the molecular mapping and QTLs against the different strains of pathogen. Moreover, the important aspects covering the related knowledge of the pathogen, its biology, variability, perpetuation, characteristics and factor affecting the disease establishment have also been summarized.
Key Words: Breeding, Disease, Fungus, Gram, Molecular marker, Screening
Ascochyta blight (AB) of chickpea caused by Ascochyta rabiei (Pass) Lab. is devastating biotic factor contributing in yield reductions worldwide (Singh et al., 1994; Singh and Sharma 1998; Siddique et al., 2000, Kaiser et al., 2000; Chongo et al., 2003; Millan et al., 2003; Sarwar et al., 2012; Sharma and Ghosh, 2016). Globally, chickpea is cultivated in the rain fed areas where the disease can vipe out all the crop under favorable conditions (Singh, 1997; Udupa and Baum, 2003; Pande et al., 2005; Wise et al., 2008). In 1911, AB was detected for the first time ever in Punjab province around the Attock region (Butler, 1918). Moving to the other regions of the globe, Morall and Mckenzie (1974) reported about AB in America from Saskatoon area and considered the import of the pathogen through seed. Till the eighties, the disease had prevailed around twenty six countries of the world (Nene, 1980).
Now it is assumed that disease exists in more than 40 countries (Bhardwaj et al., 2010; Sharma and Ghosh, 2016).
All aerial parts of chickpea can come under symptomatic stress of AB (Nene, 1984; Shtienberg et al., 2000) (Figure 1). Emerging seedlings show brown lesions at their base which actually happens due to infected seeds. Progressively, these lesions start increasing in size thus covering and girdling around the stem ultimately resulting in death of the plants. Leaflets bear irregular round to elongated shaped brown lesions showing dark red or dark brown margins. Usually circular lesions with darker margins become visible upon the green pods having concentrically and circularly arranged pycnidia. Involving the serious infections, the lesions prevail to the seeds inside the pods which become shriveled due to fungal fruiting bodies (Nene, 1982; Singh and Sharma, 1998; Akem, 1999). Distribution of AB is dependent upon the primary infections from diseased debris or infected seeds.
The mechanism of distribution is accelerated by the highly humid and rain splashed resulting in epidemic breakdown in rain fed areas leading to complete loss of the crop (Chauhan and Sinha, 1973; Alam et al., 1987; Armstrong et al., 2001). Even the resistant cultivars cannot be regarded as symptom free as they exhibit small dark spots as well but due to the genetic resistance the spots cannot progress further (Chongo and Gossen, 2003).
AB can be managed effectively via utilization of chemicals (Rehman et al., 2013) but chemicals are harmful, have residual effects and are expensive as well (Zhang et al., 2017). So the most efficient and eco-friendly way to manage the disease involves breeding host resistance against any disease (Li et al., 2015). Breeding host resistance against AB includes conventional field screening experiments (Pande et al., 2005) as well has recent molecular breeding techniques (Labdi et al., 2013). But production of resistant genotypes has become a supreme challenge due to continuous evolving new pathotypes of A. rabiei (Singh and Reddy, 1991; Sharma and Ghosh, 2016).
The review aims to sum up all the research information about the genetic resistance in chickpea against AB which may include the valuable information about symptomology, biology, variability, life cycle and different factors affecting the AB fungus during its different life stages. Furthermore, the review summarizes almost all the field screening efforts against AB and molecular breeding techniques.
Biology and variability of Aschochyta rabiei: The existence of A. rabiei in nature is categorized by two stages i.e. anamorph and teleomorph. In anamorph, pear-shaped pycnidia contain unicellular or bicellular pycnidiospores (6-23 um) produced upon conidiophores (Punithalingam and Holliday, 1972; Nene, 1982). The fungus can grow upon different nutrient media producing a cream colored mycelium having immersed brown to black pycnidia or unicellular conidia inside. The teleomorph, Didymella rabiei (Kovacheski) var. Arx (Syn. Mycosphaerella rabiei Kovacheski), belongs to bipolar heterothallic ascomycete which is involved in production of pseudothecia upon over wintered filed crop residues.
The particular stage came into the reports of Kovachevski (1936) in Bulgaria firstly, secondly in Russia (Kaiser and Okhovat, 1996), then Greece (Wilson and Kaiser, 1995), Hungary (Armstrong et al., 2001), Spain (Barve et al., 2003), Syria (Rhaiem et al., 2008), Iran (Taleei et al., 2008) and United States (Vail and Banniza, 2009). A. rabiei involves single sexual reproduction per season followed by several asexual reproductions during the parasitic phase (Trapero-Casas et al., 1996) (Figure 2). A single regulatory locus (mating-type locus) controls all the process of sexual reproduction as it acquires alternating different sequences at the mating-type loci (Barve et al., 2003; Peever et al., 2004; Rhaiem et al., 2008; Taleei et al., 2008; Vail and Banniza 2009; Atik et al., 2011). The fungus penetration occurs within 24 hours of adhesion to host surface through natural openings by forming appressoria (Pandey et al., 1987; Illarslan and Dolar 2002).
The penetration process completes within seven days when all the non lignified cells are destroyed (Kohler et al., 1995; Pandey et al., 1987; Illarslan and Dolar, 2002).
A. rabiei is highly diversified fungus having different physiological races that are becoming challenging for the breeding host resistance (Sharma and Ghosh, 2016). The difference in various isolates from different countries e.g. Pakistan, Iran, Turkey and India was recorded regarding growth, pathogenicity and colony appearance attributes (Kaiser, 1973). The various isolates of the fungus were varying in different infecting processes that may involve germ tube formation, changes in epidermal or sub epidermal cells, differing cellulose degrading enzymes etc. (Pandey et al., 1987). The genes involved in symptoms induction or infection initiation can also be variable among different isolates of the fungus as their expressions in infection generation can be slower or faster that may also be dependent upon susceptibility or resistance of the cultivar (Hanselle et al., 2001).
Three different isolates were identified from Algeria (Ag1, Ag2 and Ag3) which proved much aggressive in infection creation and expression of the genes involved in quick disease establishment (Zerrougl et al., 2007). Higher genetic variability was observed when simple sequence repeat (SSR) and mating type (MAT) markers were involved in determination of genetic structure. So management of the AB is becoming difficult due to genetic diversity of the pathogen (Nourollahi et al., 2010).
Factor affecting survival and growth rate of the fungus: Environmental conditions are very crucial in the establishment and spread of any pathogen in nature. Similarly, the environment has weighty effects upon A. rabiei as because temperature around 20AdegC positively correlates with infection establishment (Trapero-Casas and Kaiser, 1992). Moist conditions are considered very favorable for conidia oozing out and rain splashing help in dispersal to neighboring plants (Armstrong et al., 2001). Long cool and moist spells result in disease epidemics (Rehman et al., 2013; Sharma and Ghosh, 2016). Similar findings were reported by Bedi and Aujla (1969, 1970) as they mentioned that development of fruiting bodies and fungal growth occurs better at 20AdegC and when pH is in between 7.6 to 8.6.
Correspondingly, Chaube (1986) reported reduced survival of the fungus in infected seeds and germination of infected ones at high temperature in may up to 70% but survival rates and germination percentage recorded in lowering temperature of December was much low i.e. 30%. Dry heat treatment is also a responding factor which is helpful in pathogen eradication when applied at 55AdegC-65AdegC for 6-12 hours (Tripathi et al., 1987). The life span of the plant is also a significant aspect that may play a vital role in establishment of the disease as the fifteen days old plant are most susceptible to AB disease even if the pathogen stress is lower (Serrone et al., 1987).
Breeding host resistance against Ascochyta blight: Breeding host resistance is the most significant, efficient, eco friendly and relatively economic pathway to manage any disease (Li et al., 2015). So using resistant varieties is of paramount significance to obtain high yield (Sarwar et al., 2003; Hassan et al., 2012). But production of resistant genotypes has become a supreme challenge due to continuous evolving new pathotypes of A. rabiei (Singh et al., 1991; Sharma and Ghosh 2016). New strains emerge continuously in past and resulted in loss of resistance in available germplasm such as F8 (1940-1941), C 1234 (1950-1951) and C 235 (1968) lost their resistance and were banned for cultivation previously (Bedi and Agarwal, 1962; Nene, 1982). Comparing the past with the present, durable resistance in chickpea against AB had not achieved (Lichtenzveig et al., 2002). Several structural and chemical components play vital role in building up genetic resistance in chickpea to AB.
These include hydrogen-peroxide-mediated cell wall linkages, chitinase, [beta]-1,3-glucanase and thaumatin proteins, phytoalexin accumulation and detoxification and glutathione S-transferase (Cho et al., 2004; Jayakumar et al., 2005). Variation in aggression are approximately similar in A. rabiei populations as well as chickpea cultivars (Cho et al., 2004; Udupa and Baum, 2003; Chen et al., 2004; Chongo et al., 2004). The resistance reduces when cultivars enter their flowering and podding stage (Singh and Reddy, 1993; Chongo and Gossen, 2003). It is because the pathogen is not seemed to be interested in attacking upon the lignified tissues and pith parenchyma (Angelini et al., 1993; Illarslan and Dolar, 2002). Further studies revealed that the phenomena is due to the release of hydrogen peroxide in the apoplast, oxidases in Plasma membrane, oxalate oxidases and peroxidases in cell wall (Rea et al., 2002).
Two important methods are followed for breading the host resistance to AB which has been explained extensively.
1. Field Screening Accomplishments: Exploiting host plant resistance against AB by use of various filed screening strategies which may involve controlled conditions or natural conditions was explained by Pande et al. (2005). Under field conditions, environmental factors play critical role for the disease development i.e. relative humidity (RH) and temperature. Under controlled conditions, RH can be created artificially after inoculation by use of artificial mist by irrigation foggers which leads to successful disease development (Chen et al., 2005). Other important factor for disease establishment is the use of appropriate artificial inoculums concentration. Under controlled conditions, Cut-Twig screening in metal trays and seedling screening methods are commonly used. In USA, a mini dome technique invented by Chen and Muehlbauer (2003) is also proved successful regarding AB screening.
The field screening method that is adopted worldwide for identification of resistant genetic sources against the blight at large scale was developed by ICARDA (Singh et al., 1981). In this, susceptible chickpea lines are planted at frequent intervals or all around the nursery. Nursery is inoculated with diseased debris and sprinkler irrigation is provided to create humid conditions (Udupa and Baum, 2003; Chen et al., 2005). Re-inoculation of the nursery is done with artificially prepared spore suspension (Ilyas and Khan, 1986). After the disease establishment, two methods are used to assess disease severity from which first method includes comparing with 1-9 disease rating scale (Reddy and Singh, 1984). The second method includes determination of percentage of infected leaves for each plant (Kanouni et al., 2010). This second method is thought to be more objective oriented and considered superior for disease estimation (Chen et al., 2004).
These filed screening techniques have been sturdily adopted in Pakistan, India, Syria, Canada, USA, Australia and turkey for large scale experimentation.
In the early 1980s, the researchers focused upon finding genetically resistant germplasm against AB. Thousands of accessions were screened against different Ascochyta strains. Verma et al. (1981) evaluated a germplasm collection of 1258 Desi and 174 Kabuli types of chickpeas. Singh et al. (1981) conducted field screening tests of 3200 Kabuli accessions at ICARDA. Similarly, Shukla (1984) tested 1000 genotypes for resistance to AB. Furthermore, Basher and Haware (1986) screened 3360 lines while Kinaki and Dalkiran (1987) evaluated 1100 germplasm lines against AB. We reviewed that researchers from Pakistan and India conducted dozens of field screening experiments and have provided huge information regarding resistant to moderately resistant AB genotypes of chickpea. Similarly, several other scientists around the world have recorded their efforts by identification of resistant germplasm source for host breeding against Ascochyta rabiei using field screening methods.
We have tried to populate the list of almost all the documented field experimentation since 1980 to date is populated (Table 1).
2. Marker assisted breeding: Marker assisted selection (MAS) is the best way regarding single-gene traits but it can be utilized for breeding the multiple and complex traits as well but still it seemed quite difficult as fewer success has been attained in this regard (Ribaut and Hoisington, 1998; Young, 1999; Bouchez et al., 2002; Lecomte et al., 2004)(Figure 3). Disease resistance program can be accelerated by MAS and their application by DNA markers linked to resistance genes (Michelmore, 1995; Hammond-Kosack and Jones, 1997). Historical studies revealed that AB resistance is controlled by a single dominant gene (Hafiz and Ashraf, 1953; Vir et al., 1975; Eser, 1976). But Tekeoglu et al., (2000) mentioned that two quantitatively inherited recessive genes and several minor genes play vital role against AB resistance. It is not known that these two genes exhibit the same loci or different.
Further details of host pathogen interactions and resistance inheritance mechanism have been explained by Pande et al. (2005, 2010). Interaction between multiple recessive genes and a couple of dominant complementary genes were confirmed regarding inherited resistance in race 4 of Ascochyta rabiei upon fifteen chickpea genotypes (Labdi et al., 2013).
Molecular mapping of AB resistance conferring genes is an important but complex aspect to create resistant varieties. Regarding this, identification of several QTLs have been confirmed with respect to multiple linkage groups against the AB resistance. Among them, linkage group 2 bears two most important QTLs at loci GA16 and TA37 which are thought to be managing the resistance against AB pathotype 1. Linkage group 4 inhibits a couple of QTLs which are linked to TA72, CaETR. Regarding pathotype II, upon linkage group 2, a specific TA46 marker was identified for resistance in FLIP 84-92c under field conditions (Cho et al., 2004). Under controlled conditions, association of different loci i.e.TS12b and STMS28 was found upon linkage group 1(Flandez-Galvez et al., 2003a).
Further studies were conducted to understand the position of different QTLs upon the chromosomes and found that QTL1 was located upon chromosome-C, QTL2,3 upon chromosome-H and QTL4,5,6 upon chromosome-B (Bian et al., 2007). All these QTLs acquire resistance against AB Pthotype I and II and are responsible for phenotypic variation. A few years ago, fourteen micro markers linking to QTLs Ar2a,c ;Ar3c ; Ar4a,b ; Ar6b and Ar8a upon LG2,3,4,6 and LG were identified (Hamwieh et al., 2013).
Allele specific markers (CaETR, TA72, SCY17, GAA47, SCAR17590) have been proven successful for AB resistance (Castro et al., 2015; Bouhadida et al., 2013). In the same infection lesion or same crop field a lot of pathotypes of AB can be coexisting which complicate the overall picture (Peever et al., 2004). This may lead to the random mating of these pathotypes thus enhancing the genetic diversity (Barve et al., 2003). AB resistance is a quantitative trait so two disease scoring systems have been evolved for different QTLs, measuring disease reaction (Flandez-Galvez et al., 2003a). QTL1 and QTL 2 were reported for involvement in Ascochyta resistance showing 50 and 45% of blight variation within two successive years while their association with genomic regions on LG1, LG2, LG3 and LG4 is also conferred (Santra et al., 2000; Flandez-Galvez et al., 2003b; Udupa and Baum, 2003; Taran et al., 2007; Aryamanesh et al., 2010).
Among all, the significance of LG4 regarding resistance against the pathogen was reported by many scientists (Kanouni et al., 2009). All the documented findings regarding QTLs, representative linkage groups and their phenotypic variations have been described (Table 2).
3. Marker assisted back cross breeding:Marker assisted back cross breeding (MABCB) involves the selection of genotypic progeny is done by monitoring the genetic restoration along with the recombining events upon chromosomal positions (Sharma and Gosh, 2016) (Figure 4). The technique involves the modification of only the target genes without any disruption towards the neighboring genetic traits (Varshney et al., 2009). For example, the AB resistance has been introgressed into FLIP98-135C, CDC Leader and Xena cultivars by using this technique (Taran et al., 2013). Similarly in India, root traits have been incorporated for tolerance against abiotic factors in cultivar JG11 (Collard et al., 2003). Furthermore, the technique was used by Varshney et al. (2016) for the development of AB resistant genotypes by incorporation of QTL-1 and QTL-II.
A sum of eight molecular marker linked to QTLs were involved as foreground selection for desired alleles while background selection was based upon usage of high genomic recovering parents along with forty diverse SSR markers. Fourteen MAB genotypes which were resistant to AB were evolved after three selfing and backcrossing rounds successively.
Table 1. Resistant germplasm source reported via field screening efforts against Ascochyta blight.
####Resistant Germplasm Reported###References
1###CM-72 and CM- 86###Haq et al., 1981
2###2380,ICC-4475,ICC-6981,ICC-6988,ILC-202,ILC-2469,ILC-4421, NEC 138 and###Verma et al., 1981
3###ICC-1257, ICC-1069 and ICC-2160###Kalia, 1984
###ICCX830408, ICCX830602, ICCX830611, ICCX830445, ICCX830455,
4###ICCX811174, ICCX811176, ICCX811177, ICCX811184, ICCX811186 and###Khirbat et al.,1984
5###ILC-3996###Reddy and Kabbabeh, 1985
6###ILC-72, ILC-3279 and ILC-3856###Singh et al., 1984
7###ILC- 191###Puglia et al., 1985
8###ILC-3279###Crinoet al., 1985
9###EC26446, P1252-1, BRG8, PG82-1, P919 and NEC2451###Tiwari and Pandey, 1986
10###ILC-183 and 82-11###Kinaki and Dalkiran, 1987
###HPG-25, HPG-40, IIPG-41, HPG-69, HPG-89, HPG-85, H-75-35, H-86-100 and
11###Anand and Sood, 1990
12###ILC-236, ILC-484 and ILC- 484###Reddy and Singh, 1990
13###ILC-3864, ILC-3870 and ILC-4221###Pal and Singh, 1990
14###ILC-482 and ILC-3279###Reddy and Singh, 1993
15###FLIP 90-95C, ICC-4475, ICC-12004,ICC-13269, ICC-13508 and ICC-13555###Iqbal et al., 1994
16###ILC191 and CM72###Sarwar et al., 1996
###FLIP97-132C, FLIP97-227C, FLIP98-224C FLIP94-90C, FLIP95-68C, FLIP95-
17###Iqbalet al., 2002
###47C and FLIP98-231C
###F16-90 C,NCS950088,NCS950038, CMC228S,SEL96TH11488,FLIP-75C and
18###Hussain et al., 2002
19###FLIP 95-68C, FLIP 95-53C, FLIP 97-74C, FLIP 95-53C and FLIP 98-177C###Toker and Seyin, 2003
###CM1966193, CMC77S, CM843198, CM1441198, CM1223198, CC104199,
20###Alam et al., 2003
###CC106199 and CC 124100
21###Dasht###Iqbal et al., 2002
22###HOO-108 and GL92024###Dubey and Singh, 2003
23###PI 559361, PI 559363 and W6 22589###Chenet al., 2004
24###ILC-482 and Hashem###Younessi et al., 2004
25###ATC 46934, ATC46892 and ATC 46935###Nguyen et al., 2005
26###Punjab-91, Bital-98, Punjab-2000, Balkassar-2000 and Vanhar###Chaudry et al., 2005
27###FLIP97-132-C, FLIP98-226C and FLIP98-231C###Iqbal et al.,2005
###FLIP98-229C, FLIP82-150C, NCS 950204, NCS 950219, NSC 9903,
28###Paidar-x, Parbat, FLIP 00-20C, FLIP 02-18C, FLIP 02-44C, FLIP 97-120C,FLIP###Malik et al., 2005
###02-39C and FLIP 97-102C
###CMC44, FLIP90144C, FLIP91150C, CMC204S, CM149S, 950035, , FLIP9393C,
29###Atta et al., 2006
###950072, 950248, FLIP157C, CH7/99,NCS2001,CH6/99, CM218/01 and CH30/99
30###PB-91, E-19, F-81-312, CM-491-80 and NO. 215###Basher et al., 2006
###FLIP 03-42C, ICC 12004,ICC 3932, ICC 4033, ICC 6373, ICC6945, NCS 0507 and
31###Malik et al.,2006
###NCS ,AZRI-7130 and AZRI-17115
32###H97-93, 1100-256, HO1-67 and HO1-79###Waldia et al., 2006
###MCC 54, MCC 523, MCC 496, MCC 133, MCC 299, MCC528, MCC 3.11and
33###Shokouhifar et al., 2006
34###RIL58-ILC72/Cr5###Rubio et al., 2006
###03039, 03041, 03053, 03115, 03131, 03133, 03143, 03159, 93A-086, 93A-111 and
35###Ilyas et al., 2007
36###Himachal Channa 1, Himachal Channa 2, GPF 2, HPG-17, PBG1 and PBG2###Basandrai et al., 2009
37###FLIP 98-133C and FLIP 98-136C###Chandirasekaran et al., 2009
38###PI 17256 and CA0090B347C###Harveson et al., 2009
39###06025, 06026, 06027, 06031, 06035, 06040, 06041,06056, Vinhar, Bitter-98, Pb-###Ghazanfar et al., 2010
###2000 and Paidar-91
###53628, 53225, 53227, 53230, 53231, 53233, 53235 ,53244, 53380,53436,53643,
40###Iqbal et al., 2010
###54247, 53045, 53217, 53218, 53323, 53651 and 53398
41###ICCV 98813, Flipper, ICCV 05111, ICCV 98801 and Jimbour #1###Duet al., 2012
42###FLIP 97-121C###Kaur et al., 2012
###08006, 08016, 08017, 08024, 08026, 08030, 08050, 08051, 08052, 08053, 08054,
###07006, 07008, 07009, 07010, 07025, 07045, 07057, 07058, 06001, 06004, 06024,
###06040, 06052, 05007, 05030, 09001, 09030, 09005, 09006, 09007, 09009, 09011,
44###09013, 09015, 09016, 09019, 09020, 09021, 09022, 09023, 09025, 09027, 09028,###Hasan et al., 2012
###09029, 09031, 09032, 09033, 09034, 09035, 09036, 09038, 09039, 09041, 09042,
###09047, 09048, 06A003, 06A004, 05A020, 07A005, 09AG005, 93A111, 09AG009,
###09AG012, 07A003 and 07A008
45###04A09, 06A083 and 07A006###Sahi et al., 2012
###ILC182, ILC200, ILC3279, ILC3912, ILC3919, ILC3274,ILC3856, ILC7374,
46###Kaur et al.,2013
###ILC7795, PHC 15,ICC12004 and ICC12004
###CH32/02 and CH9/02, Pb2008,CM2008, CH87/02(B8/02), CH7/02, CH31/02,
47###Sarwar et al., 2012
###CH34/03, CH4/02 and CH88/03
48###EC 516934, ICCV 04537, ICCV 98818, EC 516850 and EC 516971###Pande et al., 2013
49###FLIP 4107, FLIP 1025 and FLIP 10511###Benzohra et al., 2013
50###PB-101 and PB-620###Ali et al., 2013
51###Thal-2006, Dasht and Vanher-2000###Rehman et al.,2013
52###ICC7052, ICC4463, ICC4363, ICC2884, ICC7150, ICC15294 and ICC11627###Kimurto et al., 2013
###K-60013, K-98008, D-97092, K-96001, K-96022, D-91055, D90272, D-96050,D-
53###Ahmad et al., 2013
###Pb2008 and D-Pu502-362
###ILC 200, ILC 5921, ILC 6043, ILC6090, ILC 202, ILC 2956, ILC 5586, ILC 2506,
54###ILC3279, ILC 3856 ,ILC 4421, ILC 72, ILC 182 , ILC 187, ILC72, ILC182,###Labdi et al., 2013
###ILC200, ILC442 and ILC6090
55###8032, Thal-2006, 06001 and 5CC-109###Rashid et al., 2014
56###CM-98, 1848, 6003 and 7050###Aslam et al., 2014
57###10A and 28B###Duzdemir et al.,2014
58###k01208,K-01209, K-01212 and K-01213###Jabbar et al., 2014
59###ILC72, ILC182, ILC187, ILC200 and ILC202###Benzohra et al., 2015
60###ILC 8068, ICC 4475, ILC 200, ILC 7374 and ILC 7795.###Labdi et al.,2015
61###Alef. PI 383626###Armstrong-Cho et al.,2015
###K0010-09, K0021-09, K0025-09, K0030-09, K0051-09, K0054-09, K0057-09,
###K0058-09,K0062-09, K0066-09, BKK17124, BKK07151, D080-09, D084-09,
62###Shah et al., 2015
###D089-09, D090-09, D094- 09, D095-09, BK07A005, BK96A2055, BK05A015,
###BK04A013 and FG-0908
###Moore et al., 2016,
63###CICA1007, CICA0912 and GenesisTM 425
Table (2) Markers/QTLs identified for genetic resistance of chickpea against Ascochyta blight.
####Markers/QTLs Identified###Linkage Group###References
1###UBC733b, UBC181a and Dia4###LG1 and LG6###Santra et al., 2000
2###GAA47###LG4###Tekeoglu et al., 2002
3###STMS11, GA2 and TR20###LG4###Collard et al., 2003
4###TS45, TA146 and TA130###LG1, LG2 and LG3###Flandez-Galvez et al., 2003
5###SC/OPK13-003###LG4###Millan et al., 2003
6###STMS11, GA2, GAA47 and TR20###LG4###Rakshit et al., 2003
7###Ta20,TA72 and ar1###LG2 and LG4###Udapa et al., 2003
8###GA16,GA24,GAA47 and Ta46###LG2, LG4 and LG6###Cho et al., 2004
9###H3C041, TA2, H1A12/H1H13, H1G20, H1C092###LG4 and LG8###Lichtenzveig et al., 2006
10###OPAI09746 and UBC881621###LG2###Cobos et al., 2006
11###TA194###LG4###Iruela et al., 2006
12###TA34 and TA142###LG3###Aryamanesh et al.,2007
13###TA64, TS54 and TA176###LG3, LG4 and LG6###Tar'an et al., 2007
14###TR19, TS54, TA132, TS45 and TA64###LG2, LG3, LG4 and LG8###Anbessa et al., 2009
15###TA125, TA72 and GA26###LG3, LG4 and LG6###Kanouni et al., 2009
16###TA34, TA142, STMS11, TAA170, H3D09 and###LG3 and LG4###Aryamanesh et al., 2010
17###STMS11, Ta106 and CaM0244###LG4, LG5 and LG6###Sabbavarapu et al., 2013
18###SNP_40000185, TA146 and TA72###LG4###Stephens et al., 2014
19###CaETR and GAA47###LG4###Castro et al.,2015
20###CAV1SC21.1P149511,scaffold905p1129574,CAV1###Daba et al., 2016
###494014,CAV1sc445.1p9288, CAV1SC102.1P54882###LG6, LG7 and LG8
Conclusions and Future Prospects: Managing the genetic resistance in chickpea against AB is a challenge because of high level of diversity in primary gene pool of host, complexity in molecular bases in QTLs and variable pathogen population with continuously emerging new pathotypes. Extraordinary efforts of scientist since 1980's to date has enabled us understanding the pathogen, its genetics, variability, diversity, lifecycle, perpetuation, biology, factors contributing in its survival and host screening techniques regarding resistance. Dozens of experiments were conducted in which thousands of inbread lines and cultivars were tested in different countries of the world by the scientists to keep a close eye upon the resistant germplasm sources so that the resistant genes may be identified and incorporated for durable resistance against the pathogen.
Recently, some new molecular techniques have got highlighted such as RNA interference (RNAi), next generation sequencing (NGS) and CRISPR-Cas9. These techniques are relatively simple, fast and efficient to quickly perform genetic mutations (Islam et al., 2017). Utilization of these techniques can lead towards the development of AB resistant varieties through incorporation of resistant genes from the wild types. Integration of molecular tools along with the conventional breeding approaches can boost up the gene introgression into new genotypes. Upon the linkage maps, resistance related molecular markers assisted with major QTLs have been identified which are beneficial for pyramiding the unique traits. But still we are far behind in achieving a complete AB prone environment so efforts are needed to be continued in the very way to defeat the pathogen and acquiring the desirable results.
Acknowledgements: We acknowledge the anonymous reviewers for their critical suggestions to improve the review. Furthermore, we tried to include almost all the available data regarding breeding resistance to chickpea against AB via field screening efforts and recent molecular breeding techniques however we apologize to all the other authors whose contributions have not been included in this review.
Author Contributions: WI and MQ collected, surveyed the research data and compiled the MS. AN helped in compiling tables, AI suggested about the figures while LW reviewed the article and approved for publication.
Ahmad, S., M. A. Khan, S. T. Sahi and R. Ahmad (2013). Evaluation of chickpea germplasm against Ascochyta rabiei (pass) lab. The J. Animal Plant Sci. 23: 440-443.
Akem, C.N. (1999). Ascochyta blight of chickpea: Present status and future priorities. Int. J. Pest Manage. 45:131-137.
Alam, S. S., R.N. Strange and S.H. Qureshi (1987). Isolation of Ascochyta rabiei and a convenient method for copious inoculum production. The Mycologist. 21: 20.
Alam, S. S., M. Hassan, M.A. Haq, T.M. Shah, B.M. Atta and H. Syed (2003). Screening for Ascochyta blight resistance in chickpea. Mycopath. 1(2):129-130.
Ali, Q., M. Iqbal, A. Ahmad, M.H.N. Tahir, M. Ahsan, N. Javed and J. Farooq (2013). Screening of chickpea (Cicer arietinum L.) germpalsm against Ascochyta blight [Ascochyta rabiei (Pass.) Lab.] correlation and combining ability analysis for various quantitative traits. J. Plant Breed. Crop Sci. 5(6):103-110. DOI: 10.5897/JPBCS10.041.
Anbessa, Y., B. Taran, T.D. Warkentin, A. Tullu and A. Vandenberg (2009). Genetic analyses and conservation of QTL for Ascochyta blight resistance in chickpea (Cicer arietinum L.). Theor. Appl. Genet. 4: 757-765.
Angelini, R., M. Bragaloni, R. Federico, A. Infantino and A. Porta-Poglia (1993). Involvement of polyamines, diamine oxidase and preoxidase in resistance of chickpea to Ascochyta rabiei. J. Plant Pathol. 142: 704-709.
Anand, S and B.C. Sood (1990). Screening of chickpea cultivars against Ascochyta blight in Himachal Pardesh, Ind. Chickpea newsletter. 1: 23-24.
Armstrong, C.L., G. Chongo, B.D. Gossen and L.J. Duczek (2001). Mating type distribution and incidence of the teleomorph of Ascochyta rabiei (Didymella rabiei) in Canada. Can. J. Plant Pathol. 23: 110-113.
Armstrong-Cho, C., M. Monika, Lulsdorf, P. Hashemi and S. Banniza (2015). Characterization of resistance to Ascochyta blight of selected wild Cicer germplasm. Bot. 93(11): 723-734. 10.1139/cjb-2015-0072.
Aryamanesh, N. (2007). Chickpea improvement through genetic analysis and Quantitative Trait Locus (QTL) mapping of Ascochyta blight resistance using wild Cicer species. Ph.D. Thesis, Faculty of Natural and Agricultural Sciences, University of Western Australia, Australia.
Aryamanesh, N., M.N. Nelson, G.Yan, H.J. Clarke and K.H.M. Siddique. (2010). Mapping a major gene for growth habit and QTLs for Ascochyta blight resistance and flowering time in a population between chickpea and Cicer reticulatum. Euphytica. 173: 307-319.
Aslam, M., A. Khalil, M. A. Maqbool, S. Bano, Q. Zaman and G. M. Talha (2014). Assessment of adaptability in genetically diverse chickpea genotypes (Cicer arietinum L.) based on different physio-morphological standards under ascochyta blight inoculation. Int. J. Adv. Res. 2: 245-255.
Atta, B.M., M.A. Haq, T.M. Shah, S.S. Alam, H. Ali and K.P. Akhtar (2006). Chickpea germplasm screening for resistance against Ascochyta blight. J. Bio. 18(2):137-150.
Atik, O., M. Baum., A. El-Ahmed, S. Ahmed, M.M. Abang, M.M. Yabrak, S. Murad, S. Kabbabeh and A. Hamwieh. (2011). Chickpea Ascochyta blight: Disease status and pathogen mating type distribution in Syria. J. Phytopathol. 159: 443-449.
Barve, M.P., T. Arie, S. Salimath, F.J. Muehlbauer and T.L. Peever. (2003). Cloning and characterization of the mating type (MAT) locus from Ascochyta rabiei (teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp. Fungal Gen. Biol. 39: 151-167.
Basandrai, A.K., D. Basandrai, S. Pande, P.M. Gaur, S.K. Thakur, H.L. Thakur and M. Sharma (2009). Pathotype specific seedling and adult plant resistance sources to Ascochyta rabiei in chickpea. Proceed. The 2nd International Ascochyta Workshop, June 28 - July 2, Pullman, Washington, USA.
Basher, M and M.P. Haware (1986). Screening of chickpea genotypes for the resistance to six races of blight. Chickpea newsletter. 14:27-29.
Basher, A., M. Yaqoob, M. Raheem, R. Khalid, and Naheebullah (2006). Source of resistance against disease complex in chickpea. Indus J. Biol. Sci. 3: 660-663.
Bedi, K.S. and D.S. Agarwal (1962). C 235 is the answer to blight. Indian Fmg. 12: 20-22.
Bedi, P.S and S.S. Ajula (1969). Variability in Phylosticta rabiei (pass) Trott.; The indicant of blight disease of gram. Punjab. J. Res. 6:103-106.
Bedi, P.S and S.S. Ajula (1970). Factors affecting the mycelial growth and size of pycnidia produced by the Phylosticta rabiei (pass) Trott.; The indicant of gram blight. Punjab. J. Res. 4:606-609.
Benzohra, I.E., B.S. Bendahmane, M. Labdi and M.Y. Benkada (2013). Sources of Resistance in Chickpea Germplasm to Three Pathotypes of Ascochyta rabiei (Pass.) Labr. In Algeria. World Appl. Sci. J. 21: 873-878.
Benzohra, I.E., B.S. Bendahmane, M.Y. Benkada and M. Labdi (2015). Screening of 15 chickpea germplasm accessions for resistance to Ascochyta rabiei in NorthWest of Algeria. American-Eurasian J. Agric. Environ. Sci. 15:109-114.
Bhardwaj, R., J.S. Sandhu, L. Kaur, S.K. Gupta, P.M. Gaur and R.K Varshney (2010). Genetics of Ascochyta blight resistance in chickpea. Euphytica. 171: 337-343.
Bian, X.Y., R. Ford, T.R. Han, T.E. Coram, E.C.K. Pang and P.W.J. Taylor (2007). Approaching chickpea quantitative trait loci conditioning resistance to Ascochyta rabiei via comparative genomics. Australas. Plant Pathol. 36, 419-423.
Bouchez, A., F. Hospital, M. Causse, A. Gallais and A. Charcosset (2002). Marker-assisted introgression of favorable alleles at quantitative trait loci between maize elite lines. Genetics. 162: 1945-1959.
Bouhadida, M., R. Benjannet, E. Madrid, M. Amri and M. Kharrat (2013). Efficiency of marker-assisted selection in detection of ascochyta blight resistance in Tunisian chickpea breeding lines. Phytopathol. Mediterr. 52:202-211.
Butler, E.J. (1918). Fungi and disease in plants, Bishen Singh and Mahendra Pal Singh, New Conaught Place, Dehradun Periodical Experts, 42-D, Vivek Vihar, Dehli-32. 547 pp. (reprinted 1973).
Castro, P., J. Rubio, E. Madrid, M.D. Fernandez-Romero, T. Millan and J. Gil (2015). Efficiency of marker-assisted selection for ascochyta blight in chickpea. J. Agric. Sci. 153: 56-67.
Chandirasekaran, R., T.D. Warkentin, Y. Gan, S. Shirtliffe, B.D. Gossen, B. Taran and S. Banniza. (2009). Improved sources of resistance to ascochyta blight in chickpea. Can. J. Plant Sci. 89: 107-118.
Chaube. (1986). Survival of Ascochyta rabiei in gram seed. J. Mycol. Plant Pathol. 16(2):183-184.
Chaudry, M.A., M. Faqir and A. Muhammad. (2005). Screening of chickpea germplasm for resistance of Ascochyta blight. J. Agric. Res. 43(3):229-233.
Chauhan, R.K.S. and S. Sinha (1973). Effect of varying temperature, Humidity and light during incubation in relation to disease development in blight of gram (Cicer arietinum L.) caused by Ascochyta rabiei. Proceed. National Science Academy, India. B, 37: 473-482.
Chen, W. and F.J. Muehlbauer (2003). An improved technique for virulence assay of Ascochyta rabiei on chickpea. Int. Chickpea Pigeonpea Newsletter. 10: 31-33.
Chen, W., C.J. Coyne, T.L. Peever and F.J. Muehlbauer (2004). Charactrization of Chickpea differentials for pathogenicity assay of ascochyta blight and identification of chickpea accessions resistant to Didymella rabiei. Plant Pathol. 40: 401-407.
Chen, W., K.E. Mcphee and F.J. Muehlbauer (2005). Use of a mini-dome bioassay and grafting to study resistance of chickpea to ascochyta blight. J. Phytopathol. 153: 579-587.
Cho, S., W. Chen and F.J. Muehlbauer (2004). Pathotype-specific genetic factors in chickpea (Cicer arietinum L.) for quantitative resistance to ascochyta blight. Theor. Appl. Gen. 109: 733-739.
Chongo, G. and B.D. Gossen (2003). Diseases of Chickpea. In: Diseases of Field Crops in Canada, Bailey, K.L., B.D. Gossen, R. Gugel and R.A.A. Morrall (Eds.). Canadian Phytopathological Society, Saskatoon, SK, pp: 185-190.
Chongo, G., L. Buchwaldt, B.D. Gossen, G.P. Lafond, W.E. May, E.N. Johnson and T. Hogg (2003). Foliar fungicides to manage ascochyta blight (Ascochyta rabiei) of chickpea in Canada. Can. J. Plant Pathol. 25: 135-142.
Chongo, G., B.D. Gossen, L. Buchwaldt, T. Adhikari and S.R. Rimmer (2004). Genetic diversity of Ascochyta rabiei in Canada. Plant Dis. 88: 4-10.
Cobos, M.J., J. Rubio, R.N. Strange, M.T. Moreno, J. Gil and T. Millan (2006). A new QTL for Ascochyta blight resistance in an RIL population derived from an interspecific cross in chickpea. Euphytica. 149: 105-111.
Collard, B.C.Y., E.C.K. Pang, P.K. Ades and P.W.J. Taylor (2003). Preliminary investigations of QTL associated with seedlings resistance to Ascochyta blight from Cicer echinospermum, a wild relative of chick pea. Theor. Appl. Genet. 107: 719-729.
Crino, P., A.P. Puglia and F. Saccardo (1985). Reaction of chickpea lines to Ascochyta rabiei winter sowing of Italy. Chickpea newsletter. 12: 27-29.
Daba, K., A. Deokar, S. Banniza, T. D. Warkentin and B. Tar'an (2016). QTL mapping of early flowering and resistance to ascochyta blight in chickpea. Genome. 59(6): 413-425. doi: 10.1139/gen-2016-0036
Du, W., X. Zhao, T. Raju, P. Davies and R. Trethowan (2012). Identification of Ascochyta rabiei disease resistance in chickpea genotypes. Euphytica. 186: 697-704. DOI 10.1007/s10681-011-0554-3
Dubey, S.C and B. Singh (2003). Evaluation of chickpea genotypes against Ascochyta blight. Ind. Phytopath. 56: 505.
Duzdemir, O., B. Selvi, Y. Yanar and A. Yildirimi (2014). Sources of resistance in chickpea (Cicer arietinum L.) land races against Ascochyta rabiei causal agent of ascochyta blight disease. Pakistan J. Bot. 46: 1479-1483.
Eser, D. (1976). Heritability of some important plant characters, their relationships with plant yield and inheritance of ascochyta blight resistance in chickpea (Cicer arietinum L.); Ankara University, Faculty of Agriculture Publications No. 620: Ankara, Turkey.
Flandez-Galvez, H., P.K. Ades, R. Ford, E.C.K. Pang and P.W.J. Taylor (2003a). QTL analysis for ascochyta blight resistance in an intraspecific population of chickpea. Theor. Appl. Genet. 107: 1257-1265.
Flandez-Galvez, H., R. Ford, E.C.K. Pang and P.W.J. Taylor (2003b). An intraspecific linkage map of the chickpea (Cicer arietinum L.) genome based on sequence-tagged microsatellite site and resistance gene analog markers. Theor. Applied Genet. 106: 1447-1456.
Hafiz, A and M. Ashraf (1953). Studies on inheritance of resistance to Mycosphaerella blight in gram. Phytopathol. 43: 580-581.
Hammond-Kosack, K.E. and D.J.G. Jones (1997). Plant disease resistance genes. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48: 575-607.
Hamwieh, A., M. Imtiaz, K. Hobson and S.A. Kemal (2013). Genetic diversity of microsatellite alleles located at quantitative resistance loci for Ascochyta blight resistance in a global collection of chickpea germplasm. Phytopathol. Mediterr. 52: 191-199.
Ghazanfar, M.U., S.T. Sahi, N. Javed and W. Wakil (2010). Response of advanced lines of chickpea against chickpea blight disease. Pakistan J. Bot. 42: 3423-3430.
Hanselle, T.,Y. Ichinosebb and W. Barz (2001). Biochemical and molecular biological studies on infection (Ascochyta rabiei)-induced thaumatin-like proteins from chickpea plants (Cicer arietinum L.). 1096-1107. ZNC-2001-56c-1095.pdf.
Haq, M.A., A. Shakoor, M. Sadiq and M. Hussain (1981). Induction of Ascochyta blight resistant mutant in chickpea. Mutation breeding newsletter. 17:5-6.
Harveson, R.M., C.A. Urrea and D.D. Baltensperger (2009). Evaluating chickpea genotypes for resistance to Ascochyta blight in Nebraska. Online. Plant Health Progress doi:10.1094/PHP-2009-0720-01-RS.
Hassan, S.I., A. Hameed, M.Tariq and M. Arshad (2012). Pearl: A high yielding and full season white maiz variety. J. Agric. Res. 50 (3): 329-334.
Hussain, S., M. Basir, M. Irshad and A. Bakhsh (2002). Evaluation of chickpea germplasm against Ascochyta blight. Pakistan J. Bot. 34 (4): 429-431.
Illarslan, H. and F.S. Dolar (2002). Histological and ultrastractural changes in leaves and stems of resistant and susceptible chickpea cultivars to Ascochyta rabiei. J. Phytopathol. 150: 340-348.
Ilyas, M.B. and I.H. Khan (1986). A low cost easy technique for culturing of Ascochyta rabiei fungus. Pakistan J. Agri. Sci. 23(1): 60.
Ilyas, M.B., M.A. Chaudhry, N. Javed, M.U. Ghazanfar and M.A. Khan (2007). Sources of resistance in chickpea germplasm against ascochyta blight. Pakistan J. Bot. 39:1843-1847.
Iqbal, S.M., S. Hussain and B.A. Malik (1994). Screening of chickpea lines against Ascochyta blight. ICPN. 1:21.
Iqbal, S.M., S. Hussain, A. Bakhsh and M. Bashir (2002). Sources of resistance in chickpea against ascochyta blight disease. Int. J. Agri. Biol. 4: 488-490.
Iqbal, S.M., A. Bakhsh, S.R. Malik and A.M. Haqqani (2005). Screening of chickpea lines for resistance to Ascochyta blight. Chickpea and pigeonpea newsletter. 12:34-35.
Iqbal, S. M., A. Ghafoor, A. Bakhsh, I. Ahmad and A. Sher (2010). Identification of resistant sources for multiple disease resistance in chickpea. Pakistan J. Phytopathol. 22(2): 89-94.
Islam, W., S.U. Islam. M. Qasim and L. Wang (2017). Host-Pathogen interactions modulated by small RNAs, RNA Biol. 14 (7): 891-904. http://dx.doi.org/10.1080/15476286.2017.1318009
Iruela, M., J. Rubio, F. Barro, J.I. Cubero, T. Millan and J. Gil (2006). Detection of two QTLs for resistance to ascochyta blight in an intraspecific cross of chickpea (Cicer arietinum L.): Development of SCAR markers associated to resistance. Theor. Applied Genet. 112: 278-287.
Jabbar, A., S.A. Khan, M. Iqbal, N. Javed, M.U. Shahbaz and M. Iqbal (2014). Determination of resistance in chickpea germplasm against Ascochyta rabiei (pass l.) And its chemical management. Pakistan J. Phytopathol. 26 (01): 75-83.
Jayakumar, P., B.D. Gossen, Y.T. Gan, T.D. Warkentin and S. Banniza (2005). Ascochyta blight of chickpea: Infection and host resistance mechanisms. Can. J. Plant Pathol. 27: 499-509.
Kaiser, W.J. (1973). Factors affecting growth, Sporulation, Pathogenesity and survival of Ascochyta rabiei. Mycologist. 65: 444-457.
Kaiser, W.J and M. Okhovat (1996). Distribution of Didymella rabiei, the teleomorph of Ascochyta rabiei in Iran. Iran. J. Plant Pathol. 32: 158-162.
Kaiser, W.J., M.D. Ramsey, K.M. Makkouk, T.W. Bretag, N. Acikgoz, J. Kumar and F.W. Nutter (2000). Foliar diseases of cool season food legumes and their control. In Linking Research and Marketing Opportunities for Pulses in the 21st Century; Knight, R., Ed.; Kluwer Academic Publishers: Dordrecht, The Netherlands., pp. 437-455.
Kalia, N.B. (1984). Chickpea improvement in Himachal Pardesh. Chickpea newsletter. 11:9.
Kanouni, H., A. Taleei, S.A. Peyghambari, S.M. Okhovat, M. Baum and M. Abang (2009). QTL analysis for ascochyta blight resistance in chickpea (Cicer arietinum L.) using microsatellite markers. Seed Plant J. Agric. Res. 25: 109-127.
Kanouni, H., A. Taleei, S.A. Peyghambari, S M. Okhovat and M. Abang (2010). Impact of different pathotypes and spore concentrations on Ascochyta blight incidence in two chickpea (Cicer arietinum L.) genotypes. Iranian J. Crop Sci. 12: 56-68.
Kaur, l., J.S. Sandhu, R.S. Malhotra, M. Imtiaz and A. Sirari (2012). Sources of stable resistance to Ascochyta blight inexotic kabuli chickpea. J. Food Legumes. 25: 79-80.
Kaur, L., A. Sirari, D. Kumar, J. S. Sandhu, S. Singh, K. Kapoor, I. Singh, C.L. Laxmipa, T. Gowda, S. Pande, P. Gaur, M. Sharma, M. Imtiaz and K.H.M. Siddique (2013). Combining Ascochyta blight and Botrytis grey mould resistance in chickpea through interspecific hybridization. Phytopathologia Mediterranea . 52(1): 157-165.
Kimurto, P.K., B.K. Towett, R.S. Mulwa, N. Njogu, L.J. Jeptanui, G.N.V.P.R. Rao, S. Silim, P. Kaloki, P. Korir and J.K. Macharia (2011). Evaluation of chickpea genotypes for resistance to Ascochyta blight (Ascochyta rabiei)disease in the dry highlands of Kenya. Phytopathol. Mediterr. 52: 212-221.
Kinaki, E and H. Dalkiran (1987). Resistant source indication against Ascochyta blight of chickpea in central Anatolian region. Turk. J. Phytopathol. 16(1):9-15.
Khirbat, S.K., H. Chand, B.L. Jalali, J. Kumar And Y.L. Nene (1984). Further screening of chickpea genotypes for ascochyta blight resistance. 7 chapter ii review of literature. The research conducted on Blight. Available at : https.prr.hec.gov.pk/Chapters/811S-2.pdf (Accessed: March, 2016)
Kohler, G., C. Linkert and W. Barz (1995). Infection studies of Cicer arietinum (L.) with gus (E. coli beta-glucuronidase) transformed Ascochyta rabiei strains. J. Phytopathol. 143: 589-595.
Kovachevski, I.C. (1936). The blight of chickpea (Cicer aritinum). Mycosphaerella rabiei f.sp. Cicri. Ministry of Agriculture and National Domains, Plant Protection Institute, Sofia, Bulgaria.
Labdi, M., R.S. Malhotra, I.E. Benzohra and M. Imtiaz (2013). Inheritance of resistance to Ascochyta rabiei in 15 chickpea germplasm accessions. Plant Breed. 132: 197-199.
Labdi, M., S. Ghomari and S. Hamdi (2015). Combining ability and gene action estimates of eight parent diallel crosses of chickpea for Ascochyta Blight. Adv. Agriculture. http://dx.doi.org/10.1155/2015/832597
Lecomte, L., P. Duffe, M. Buret, B. Servin, F. Hospital and M. Causse (2004). Marker-assisted introgression of five QTLs controlling fruit quality traits into three tomato lines revealed interactions between QTLs and genetic backgrounds. Theor. Appl. Genet. 109:658-668.
Li, H., M. Rodda, A. Gnanasambandam, M. Aftab, R. Redden, K. Hobson, G. Rosewarne, M.Materne, S.Kaur and A.T. Slater (2015). Breeding for biotic stress resistance in chickpea: Progress and prospects. Euphytica. 204: 257-288.
Lichtenzveig, J., D. Shtienberg, H.B. Zhang, D.J. Bonfil and S. Abbo (2002). Biometric analyses of the inheritance of resistance to Didymella rabiei in chickpea. Phytopathology. 92: 417-423.
Lichtenzveig, J., D.G. Bonfil, H.B. Zhang, D. Shtienberg and S. Abbo (2006). Mapping quantitative trait loci in chickpea associated with time to flowering and resistance to Didymella rabiei the causal agent of Ascochyta blight. Theor. Appl. Genet. 113: 1357-1369
Malik, S.R.,S.M. Iqbal, U. Iqbal, I. Ahmad and A.M. Haqqani (2005). Response of chickpea lines to ascochyta rabiei at two growing stages. Caspian J. Env. Sci. 3: 173-177.
Malik, S.R., S.M. Iqbal and A.M. Haqqani 2006. Resistance screening to Ascochyta blight disease of chickpea in Pakistan. Chickpea and pigeon pea newsletter. 13: 30-31.
Michelmore, R. (1995). Molecular approaches to manipulation of disease resistance genes. Annu. Rev. Phytopathol. 33: 393-427.
Millan, T., J. Rubio, M. Iruela, K. Daly, J.I. Cubero and J. Gil (2003). Markers associated with Ascochyta blight resistance in chickpea and their potential in marker-assisted selection. Field Crops Res. 84: 373-384.
Moore, K., K. Hobson, S. Harden, P. Nash, G. Chiplin and S Bithell (2015). Effect of chickpea ascochyta on yield of current varieties and advanced breeding lines. Department of Primary Industries, NSW, Tamworth. Available at: https://grdc.com.au/Research-and-Development/GRDC-Update-Papers/2015/02/Effect-of-chickpea-ascochyta-on-yield-of-current-varieties-and-advanced-breeding-lines
Morrall, R.A.A and D.L. Mackenzie (1974). A note on the inadvertent introduction of Ascochyta rabiei to North America, A destructive chickpea disease, Plant Disease Reporter. 58(4): 342-345.
Nguyen, T.T., P.W.J. Tylor, R.J. Redden and R. Ford (2005). Resistance to Ascochyta rabiei in a wild Cicer germplasm under field conditions. Aus. J. Exp. Agric. 45(10):1291-1296.
Nene, Y.L. (1980). Diseases of chickpea. Proceed. International workshop on chickpea improvement. ICRISAT, 28 Feb to 1st Mar. India., 171-178.
Nene, Y.L. (1982). A review of Ascochyta blight of chickpea. Trop. Pest Manag. 28: 61-70.
Nene, Y.L. (1984). A review of ascochyta blight of chickpea (Cicer arietinum L.). In: ascochyta blight and winter sowing of chickpeas, Sexena, M.C. and K.B. Singh (Eds.). Martinus Nijhoff/ DR W. Junk Publishers, The Netherlands. 17-33.
Nourollahi, K., M. Javannikhah, M.R. Naghavi, J.L. Lichtenzveig, S.M. Okhovat, R.P. Oliver and S.R. Ellwood. (2010). Genetic diversity and population structure of Ascochyta rabiei from the western Iranian Ilam and Kermanshah provinces using MAT and SSR markers. Mycological Progress. 10 (1): 1-7.
Pal, M and B. Singh (1990). Screening of gram germplasm against Ascochyta rabiei. Ind. J. Mycol. Plant pathol. 20(1): 60-61.
Pandey, B.K., U.S. Singh and H.S. Chaube (1987). Mode of infection of ascochyta blight as caused by Ascochyta rabiei. J. Phytopathol. 119: 88-93.
Pande, S., K.H.M. Siddique, G.K. Kishore, B. Bayaa, P.M. Gaur, C.L.L. Gowda, T.W. Bretag, and J.H. Crouch (2005). Ascochyta blight of chickpea (Cicer arietinum L.): A review of biology, pathogenicity and disease management. Aust. J. Agric. Res. 56: 317-332.
Pande, S., M. Sharma, P.M. Gaur and C.L.L. Gowda (2010). Host plant resistance to ascochyta blight of chickpea; information bulletin no 82; international crops research institute for the semi-arid tropics: Patancheru, India. p40.
Pande, S., M. Sharma, P.M. Gaur, A.K. Basandrai, L. Kaur., K.S. Hooda, D. Basandrai, T. Kiran Babu, S.K. Jain and S.K. Rathore (2013). A. Biplot analysis of genotype _ environment interactions and identification of stable sources of resistance to Ascochyta blight in chickpea (Cicer arietinum L.). Australas. Plant Pathol. 42: 561-571.
Peever, T.L., S.S. Salimath, G. Su, W.J. Kaiser and F.J. Muehlbauer (2004). Historical and contemporary multilocus population structure of Ascochyta rabiei (teleomorph: Didymella rabiei) in the Pacific Northwest of the United States. Mol. Ecol. 13: 291-309.
Puglia, A.P., P. Crino, F. Saccardo and G. Cechini (1985). Differential behavior of chickpea lines towards some isolates of Ascochyta rabiei. Chickpea newsletter. 12:22-23.
Punithalingam, E and P. Holliday (1972). Ascochyta rabiei. In descriptions of pathogenic fungi and bacteria; commonwealth mycological institute: Kew, UK., 34: p337.
Rehman, A., S. Mehboob, W. Islam and N.A. Khan (2013). Reaction of gram (Cicer Arietinum L.) Varieties against gram blight disease (Didymella Rabiei (Kovatsch.) Arx) and its management through foliar fungicides in rain fed areas of Pakistan. Pakistan J. Phytopathol. 25 (01): 07-14.
Rakshit, S., P. Winter, M. Tekeoglu, J.J. Munoz and T. Pfaff (2003). DAF Marker tightly linked to a major locus for Ascochyta blight resistance in chickpea (Cicer arietinum L.). Euphytica. 132: 23-30.
Rashid, A., M. U. Younas., M. Ehetisham-Ul-Haq, M. Farooq, I.H. Waris, E. Perveez., F. Ahmad, M. Latif and M. Ahmad (2014). Screening of chickpea (Cicer arietinum l.) varieties against ascochyta blight and its management through biopesticides. Arch. Phytopathol. Pl. Protect. 47: (8) 938-943. DOI: 10.1080/03235408.2013.826540.
Rea, G., O. Metoui, A. Infantino, R. Federico and R. Angelini (2002). Copper amine oxidase expression in defense responses to wounding and Ascochyta rabiei invasion. Plant Physiol. 128: 865-875.
Reddy, M.V. and K.B. Singh (1984). Evaluation of a world collection of chickpea germplasm lines for resistance to ascochyta blight. Plant Dis. 68: 900-901.
Reddy, M.V. and S. Kabbabeh (1985). Pathogenic variability in Ascochyta rabiei (Pass.) Lab. in Syria and Lebanon. Phytopathol. Mediterrenean. 24: 265-266.
Reddy, M.V and K.B. Singh (1990). Relationship between temperature, relative humidity and Ascochyta blight development in winter sown chickpea in Syria. J. Phytopathol. 29(3):159-162.
Reddy, M.V and K.B. Singh (1993). Reducing resistance to Ascochyta blight in chickpea. Plant Dis. 77:231-233.
Ribaut, J.M and D. Hoisington (1998). Marker-assisted selection: new tools and strategies. Trends Plant Sci. 3: 236-239.
Rhaiem, A.,M. Cherif, T.L. Peever and P.S. Dyer (2008). Population structure and mating system of Ascochyta rabiei inTunisia: Evidence for the recent introduction of mating type 2. Plant Pathol. 57: 540-555.
Rubio, J., M.T. Moreno, A. Moral, D. Rubiales and J. Gil (2006). Registration of RIL58-ILC72/Cr5, a chickpea germplasm line with rust and Ascochyta blight resistance. Crop Sci. 46: 2331-2332.
Sabbavarapu, M.M., M. Sharma, S.K. Chamarthi, N. Swapna, A. Rathore, M. Thudi, P.M. Gaur, S. Pande, S.Singh and L. Kaur (2013). Molecular mapping of QTLs for resistance to Fusarium wilt (race 1) and Ascochyta blight in chickpea (Cicer arietinum L.). Euphytica. 93: 121-133.
Sahi, M.S.T., M. Burhan, T. Iqbal and N. Sarwar (2012). Screening of chickpea breeding material and varieties/elite lines against blight (Ascochyta Rabiei) J. Agric. Res. 50(4): 517-523.
Santra, D.K., M. Tekeoglu, M. Ratnaparkhe, W.J. Kaiser and F.J. Muehlbauer (2000). Identification and mapping of QTLs conferring resistance to ascochyta blight in chickpea. Crop Sci. 40: 1606-1612.
Sarwar, M., F. Farhat, J. Nasir and A. Baig (1996). Response of four chickpea varieties against Ascochyta rabiei and their histological studies. Pakistan J. Bot. 28(2): 213-217.
Sarwar, G. and M. Ahmad (2003). Development of a new high mung bean variety "AEM-96" through induced mutations. SAARC. J. Agric. 1:173-180.
Sarwar, N., K.P. Akhtar, T.M. Shah and B.M. Atta (2012). Evaluation of chickpea advance genotypes against blight and wilt diseases under field conditions. Int. J. Agric. Biol. 14: 993-996.
Serrone, P.D., A. Porta-Puglia and P. Crino (1987). Behavior of Cicer arietinum towards Ascochyta rabiei; Effect of age and concentration of the inoculum. Informatory phytopathol. 37(6): 59-61.
Sharma, M and R. Ghosh (2016). An update on genetic resistance of chickpea to ascochyta blight. Agronomy. 6: 18; doi:10.3390/agronomy6010018
Shah, T.M., M. Imran, B. M. Atta, M. Shafiq, M. Aslam and K. Hussain (2015). Screening of chickpea advanced lines for sources of resistance against blight and wilt two major diseases of chickpea. Pakistan J. Bot. 47(6): 2443-2448.
Shokouhifar, F., A.A.R. Bagheri and F.M. Rastgar (2006). Identification of resistant chickpea lines against pathotypes causing ascochyta blight disease in Iran. Iranian J. Biol. 19: 29-42.
Shtienberg, D., H. Vintal, S. Brener and B. Retig (2000). Rational management of Didymella rabiei in chickpea by integration of genotype resistance and postinfection application of fungicides. Phytopathology. 90: 834-842.
Siddique, K.H.M., R.B. Brindsmead, R. Knight, E.J. Knights, J.G. Paull and I.A. Rose (2000). Adaptation of chickpea (Cicer arietinum L.) and Faba bean (Vicia faba L.) to Australia. In: 'Linking Research and Marketing Opportunities for Pulses in the 21st Century'. Ed. R knight, Kluwer, Academic Publishers, Dordrecht, The Netherlands, 289303.
Singh, K.B., G.C. Hawtin, Y.L. Nene and M.V. Reddy (1981). Resistance in chickpeas to Ascochyta blight. Plant Dis. 65: 586-587.
Singh, G., M.M. Verma., A.S. Gill, T.S. Sandhu, H.S. Brar, S.S. Sra and S. Kapoor (1984). Screening of gram varieties against Ascochyta blight. Crop Imp. 11: 153-154.
Singh, K.B and M.V. Reddy (1991). Advances in disease resistance breeding in chickpea. Adv. Agron. 45: 191-222.
Singh, K.B. and M.V. Reddy (1993). Resistance to six races of Ascochyta rabiei in the world germplasm collection of chickpea. Crop Sci. 33: 186-189.
Singh, K.B., R.S. Malhotra, M.H. Halila, E.J. Knights and M.M. Verma (1994). Current status and future strategy in breeding chickpea for resistance to biotic and abiotic stresses. Euphytica. 73: 137-149.
Singh, K.B. (1997). Chickpea Breeding. In: The Chickpea, Saxena, M.C. and K.B. Singh (Eds.). CAB International, Wallingford, UK, pp: 127-162.
Singh, G. and Y.R. Sharma (1998). Ascochyta blight of chickpea. In IPM System in Agriculture: Pulses; Upadhyay, R.K., Mukherji, K.G., Eds.; Aditya Books Pvt. Ltd.: New Delhi, India, pp. 163-195.
Shukla, A., B.P. Pandy and Y.P.S. Rathi (1984). Screening of chickpea germplasm against Ascochyta blight. Chickpea newsletter. 11:28-29.
Stephens, A., M. Lombardi, N.O.I. Cogan, J.W. Forster, K. Hobson, M. Materne and S. Kaur (2014). Genetic marker discovery, interspecific linkage map construction and quantitative trait locus analysis of ascochyta blight resistance in chickpea (Cicer arietinum L.). Mol. Breed. 33: 297-313.
Taleei, A., H. Kanouni, M. Baum, S.A. Peyghambari, S.M. Okhovat and M. Abang (2008). Identification and mapping of QTLs for resistance to ascochyta blight (Pathotype III) in chickpea. Proceedings of the 2nd International Conference on Future Generation Communication and Networking, (ICFGCN'08), Hainan Island, FGCN, China. 51-54.
Taran, B., T.D. Warkentin, A. Tullu and A. Vandenberg (2007). Genetic mapping of ascochyta blight resistance in chickpea (Cicer arietinumL.) using a simple sequence repeat linkage map. Genome. 50: 26-34.
Taran, B., T.D. Warkentin and A. Vandenberg (2013). Fast track genetic improvement of Ascochyta blight resistance and double podding in chickpea by marker-assisted backcrossing. Theor Appl Genet. 126: 1639-1647.
Tekeoglu, M., D.K. Santra, W.J. Kaiser and F.J. Muehlbauer (2000). Ascochyta blight resistance inheritance in three chickpea recombinant inbred line populations. Crop Sci. 40: 1251-1256.
Tekeoglu, M., P.N. Rajesh and F.J. Muehlbauer (2002). Integration of sequence tagged microsatellite sites to the chickpea genetic map. Theor. Appl. Genet. 105: 847-854.
Tiwari, S.K and M.P. Pandey (1985). Genetics of resistance to ascochyta blight in chickpea (Cicer arietinum L.). Euphytica. 35 (1): 211-215.
Toker, C and H. Seyin (2003). Selection of chickpea genotypes for resistance to ascochyta blight, yield and yield criteria. Turk. J. Agric. 27:277-283.
Tripathi, H.S., R.S. Singh and H.S. Chaube (1987). Effect of sun drying on the recovery of Ascochyta rabiei from infected chickpea seeds. Chickpea newsletter. 16:13-14.
Trapero-Casas, A. and W.J. Kaiser (1992). Development of Didymella rabiei, the teleomorph of Ascochyta rabiei, on chickpea straw. Phytopathology. 82: 1261-1266.
Trapero-Casas, A., J.A. Navas-Cortes and R.M. Jimenez-Diaz (1996). Airborne ascospores of Didymella rabiei as a major primary inoculum for Ascochyta blight epidemics in chickpea crops in southern Spain. Eur. J. Plant Pathol. 102: 237-245.
Udupa, S.M. and M. Baum (2003). Genetic dissection of pathotype-specific resistance to ascochyta blight disease in chickpea (Cicer arietinum L.) using microsatellite markers. Theor. Applied Genet. 106: 1196-1202.
Vail, S and S. Banniza (2009). Molecular variability and mating-type of Ascochyta rabiei of chickpea from Saskatchewan,Canada. Aust. J. Plant Pathol. 38: 392-398.
Varshney, R.K., S.N. Nayak, G.D. May and S.A. Jackson (2009). Next generation sequencing technologies and their implications for crop genetics and breeding. Trends Biotechnol. 27: 522-530.
Varshney, R.K. (2016). Exciting journey of 10 years from genomes to fields and markets: Some success stories of genomics-assisted breeding in chickpea, pigeonpea and groundnut. Plant Sci. 242: 98-107.
Verma, M.M., G. Singh, T.S. Sandhu, H. Singh, S.S. Sandhu, K. Singh and F. Bhullar (1981). Sources of resistance to gram blight and grey mold. Chickpea newsletter. 4:14-16
Vir, S., J.S. Grewal, J.S and V.P. Gupta (1975). Inheritance of resistance to Ascochyta blight in chickpea. Euphytica. 24:209-211.
Waldia, R.S., B.P.S. Malik, C. Hairi., V.S. Lather, I.S. Solanki and R.K. Yadav (2005). Identification of chickpea genotypes with combined resistance to Ascochyta blight and Fusarium wilt. Chickpea newsletter. 12: 11-12.
Wilson, A.D. and W.J. Kaiser (1995). Cytology and sexual compatibility in didymella rabiei. Mycologia. 87: 795-804.
Wise, K.A., C.A. Bradley, J.S. Pasche and N.C. Gudmestad (2008). Baseline sensitivity of Ascochyta rabiei to Azoxystrobin, Pyraclostrobin and Bosacalid. Plant Dis. 92: 295-300.
Younessi H., S.M. Okhovvat, G.A. Hadjaroud, S.J. ZAD and A.R. Talei (2004). Virulence variability of Ascochyta rabiei isolates on chickpea cultivars in Kermanshah province. Iran. J. Pl. Pathol. 39: 213-230.
Young, N.D. (1999). A cautiously optimistic vision for marker-assisted breeding. Mol. Breeding. 5: 505-510.
Zerroug1, M.M., Z. Bouznad, L. Larous and R.N. Strange (2007). Production of solanapyrone a by Algerian isolates of Ascochyta rabiei as the cause of the toxicity of culture filtrates to chickpea (Cicer arietinum) seeds and seedlings. J. Plant Pathol. 89(2): 227-232.
Zhang, H., T. Breeze, A. Bailey, D. Garthwaite, R. Harrington and S.G. Potts (2017). Arthropod Pest Control for UK Oilseed Rape - Comparing Insecticide Efficacies, Side Effects and Alternatives. PLoS ONE. 12(1): e0169475. https://doi.org/10.1371/journal.pone.0169475
|Printer friendly Cite/link Email Feedback|
|Publication:||Journal of Animal and Plant Sciences|
|Date:||Dec 31, 2017|
|Previous Article:||IMPACT OF GRAZING ON PLANT BIODIVERSITY OF DESERT AREA OF DISTRICT KHAIRPUR, SINDH, PAKISTAN.|
|Next Article:||IMPROVEMENT LEVEL OF NEMATODE RESISTANCE IN POTATO FACING CHANGES IN POPULATION OF GLOBODERA SPP.|